TY  - CONF
AU  - Parac-Vogt, Tatjana
AU  - Savić, Nada
AU  - Kerckhofs, Greet
AU  - Stojanović, Marko
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12940
AB  - Due to the high complexity and heterogeneous structure of biological tissues, imaging techniques that allow for precise and quantitative structural analyses of such materials are of high importance. These techniques could also advance clinical translation of regenerative medicine by providing better insights in tissue development and disease. The standard techniques for evaluating biological tissues such as histological sectioning and staining, have a high discriminative power, but allow assessment of the tissue distribution only in two dimensions. Due to restricted sectioning orientation and limited depth resolution, this leads to loss of information in three dimensions (3D), and therefore, more precise imaging of the 3D microstructure and spatial interrelationships of the different tissues within organs is crucial. MicroCT can provide full 3D structural information of mineralized tissues and dense biomaterials. However, the intrinsic low X-ray absorption of soft tissues requires contrast-enhancing staining agents (CESAs) to be used. We have shown that a range of polyoxometalate clusters (POMs) can be excellent non-destructive staining agents for high-resolution contrast-enhanced microCT (CE-CT) visualization of various tissues, of bone and its marrow vascularization and adiposity. A range of Wells-Dawson POMs, differing in structure and overall charge, has been synthesized and evaluated for their potential as soft tissue CESAs. We have shown that hafnium-substituted POM (Hf-POM) allows for simultaneous contrast-enhanced microCT (CE-CT) visualization of bone and its marrow vascularization and adiposity. Monolacunary Wells-Dawson POM (Mono-WD POM) showed similar soft tissue enhancement as Hf-WD POM and phosphotungstic acid (PTA), a frequently used but destructive CESA. However, compared to PTA, the POMs are much less destructive and show a better diffusion. The solubility of Mono-WD POM can be improved by simple addition of lithium chloride to the staining solution, leading to further enhancement of the soft tissue contrast. In vivo toxicity of Wells-Dawson POM has been also evaluated according to standard toxicological protocols using Wistar albino rats, which is the first and important step in evaluating side effects of these polyoxometalate nanoclusters that show large potential as therapeutics and contrast agents.
C3  - Medical Research : Medicinska istraživanja
T1  - Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography
VL  - 56
IS  - 4
SP  - 129
EP  - 129
DO  - 10.5937/medi56-47579
ER  - 

@conference{
author = "Parac-Vogt, Tatjana and Savić, Nada and Kerckhofs, Greet and Stojanović, Marko and Čolović, Mirjana and Krstić, Danijela",
year = "2023",
abstract = "Due to the high complexity and heterogeneous structure of biological tissues, imaging techniques that allow for precise and quantitative structural analyses of such materials are of high importance. These techniques could also advance clinical translation of regenerative medicine by providing better insights in tissue development and disease. The standard techniques for evaluating biological tissues such as histological sectioning and staining, have a high discriminative power, but allow assessment of the tissue distribution only in two dimensions. Due to restricted sectioning orientation and limited depth resolution, this leads to loss of information in three dimensions (3D), and therefore, more precise imaging of the 3D microstructure and spatial interrelationships of the different tissues within organs is crucial. MicroCT can provide full 3D structural information of mineralized tissues and dense biomaterials. However, the intrinsic low X-ray absorption of soft tissues requires contrast-enhancing staining agents (CESAs) to be used. We have shown that a range of polyoxometalate clusters (POMs) can be excellent non-destructive staining agents for high-resolution contrast-enhanced microCT (CE-CT) visualization of various tissues, of bone and its marrow vascularization and adiposity. A range of Wells-Dawson POMs, differing in structure and overall charge, has been synthesized and evaluated for their potential as soft tissue CESAs. We have shown that hafnium-substituted POM (Hf-POM) allows for simultaneous contrast-enhanced microCT (CE-CT) visualization of bone and its marrow vascularization and adiposity. Monolacunary Wells-Dawson POM (Mono-WD POM) showed similar soft tissue enhancement as Hf-WD POM and phosphotungstic acid (PTA), a frequently used but destructive CESA. However, compared to PTA, the POMs are much less destructive and show a better diffusion. The solubility of Mono-WD POM can be improved by simple addition of lithium chloride to the staining solution, leading to further enhancement of the soft tissue contrast. In vivo toxicity of Wells-Dawson POM has been also evaluated according to standard toxicological protocols using Wistar albino rats, which is the first and important step in evaluating side effects of these polyoxometalate nanoclusters that show large potential as therapeutics and contrast agents.",
journal = "Medical Research : Medicinska istraživanja",
title = "Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography",
volume = "56",
number = "4",
pages = "129-129",
doi = "10.5937/medi56-47579"
}

Parac-Vogt, T., Savić, N., Kerckhofs, G., Stojanović, M., Čolović, M.,& Krstić, D.. (2023). Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography. in Medical Research : Medicinska istraživanja, 56(4), 129-129.
https://doi.org/10.5937/medi56-47579

Parac-Vogt T, Savić N, Kerckhofs G, Stojanović M, Čolović M, Krstić D. Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography. in Medical Research : Medicinska istraživanja. 2023;56(4):129-129.
doi:10.5937/medi56-47579 .

Parac-Vogt, Tatjana, Savić, Nada, Kerckhofs, Greet, Stojanović, Marko, Čolović, Mirjana, Krstić, Danijela, "Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography" in Medical Research : Medicinska istraživanja, 56, no. 4 (2023):129-129,
https://doi.org/10.5937/medi56-47579 . .