TY  - JOUR
AU  - Ajdžanovic, Vladimir
AU  - Medigovic, Ivana
AU  - Živanović, Jasmina
AU  - Šošić-Jurjević, Branka
AU  - Trifunovic, Svetlana
AU  - Tanić, Nasta
AU  - Milošević, Verica
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/97
AB  - Somatopause, the complex aspect of andropause, is recognizable by reduced growth hormone - GH/insulin-like growth factor 1 axis function in the ageing male. Soy isoflavones (usually genistein and daidzein), which are known for their beneficial effects in the treatment of ageing symptoms, are active in the pituitary, as well. The iromuno-histomorphometric and fluorescent characteristics of pituitary growth hormone secreting cells, in an animal model of andropause, were examined after a treatment with genistein or daidzein. Andropausal Wistar rats were divided into sham operated, orchidectomized and genistein or daidzein treated orchidectomized groups. Genistein or daidzein (30 mg/kg/day) were administered subcutaneously for three weeks, while sham operated and orchidectomized groups received the vehicle alone. Growth hormone secreting cells were identified by the percoxidase-antiperoxidase immuno-histochemical, and inmuno-fluorescent procedure. The main characteristic of growth hormone secreting cells in soy isoflavones treated groups is a weaker immuno-histochemical staining and immuno fluorescent signal compared to sham operated and orchidectomized groups. The growth hormone secreting cell volume in orchidectomized +genistein or +daidzein groups is by 13.8% and 11.9% (p LT 0.05) smaller respectively, in comparison with the orchidectomized group. In orchidectomized +genistein or +daidzein groups, the growth hormone secreting cells relative volume density is by 62.5% and 61.0% lower (p LT 0.05) respectively than for the sham operated group, and decreased by 65.4% and 64.0% (p LT 0.05) respectively, compared to the orchidectomized group. It can be concluded that chronic genistein or daidzein treatment, in an animal model of andropause, attenuates immuno-histomorphometric and fluorescent characteristics of growth hormone secreting cells.
T2  - Acta Veterinaria, Beograd
T1  - Immuno-Histomorphometric and -Fluorescent Characteristics of Gh Cells After Treatment with Genistein Or Daidzein in An Animal Model of Andropause
VL  - 64
IS  - 1
SP  - 93
EP  - 104
DO  - 10.2478/acve-2014-0010
ER  - 

@article{
author = "Ajdžanovic, Vladimir and Medigovic, Ivana and Živanović, Jasmina and Šošić-Jurjević, Branka and Trifunovic, Svetlana and Tanić, Nasta and Milošević, Verica",
year = "2014",
abstract = "Somatopause, the complex aspect of andropause, is recognizable by reduced growth hormone - GH/insulin-like growth factor 1 axis function in the ageing male. Soy isoflavones (usually genistein and daidzein), which are known for their beneficial effects in the treatment of ageing symptoms, are active in the pituitary, as well. The iromuno-histomorphometric and fluorescent characteristics of pituitary growth hormone secreting cells, in an animal model of andropause, were examined after a treatment with genistein or daidzein. Andropausal Wistar rats were divided into sham operated, orchidectomized and genistein or daidzein treated orchidectomized groups. Genistein or daidzein (30 mg/kg/day) were administered subcutaneously for three weeks, while sham operated and orchidectomized groups received the vehicle alone. Growth hormone secreting cells were identified by the percoxidase-antiperoxidase immuno-histochemical, and inmuno-fluorescent procedure. The main characteristic of growth hormone secreting cells in soy isoflavones treated groups is a weaker immuno-histochemical staining and immuno fluorescent signal compared to sham operated and orchidectomized groups. The growth hormone secreting cell volume in orchidectomized +genistein or +daidzein groups is by 13.8% and 11.9% (p LT 0.05) smaller respectively, in comparison with the orchidectomized group. In orchidectomized +genistein or +daidzein groups, the growth hormone secreting cells relative volume density is by 62.5% and 61.0% lower (p LT 0.05) respectively than for the sham operated group, and decreased by 65.4% and 64.0% (p LT 0.05) respectively, compared to the orchidectomized group. It can be concluded that chronic genistein or daidzein treatment, in an animal model of andropause, attenuates immuno-histomorphometric and fluorescent characteristics of growth hormone secreting cells.",
journal = "Acta Veterinaria, Beograd",
title = "Immuno-Histomorphometric and -Fluorescent Characteristics of Gh Cells After Treatment with Genistein Or Daidzein in An Animal Model of Andropause",
volume = "64",
number = "1",
pages = "93-104",
doi = "10.2478/acve-2014-0010"
}

Ajdžanovic, V., Medigovic, I., Živanović, J., Šošić-Jurjević, B., Trifunovic, S., Tanić, N.,& Milošević, V.. (2014). Immuno-Histomorphometric and -Fluorescent Characteristics of Gh Cells After Treatment with Genistein Or Daidzein in An Animal Model of Andropause. in Acta Veterinaria, Beograd, 64(1), 93-104.
https://doi.org/10.2478/acve-2014-0010

Ajdžanovic V, Medigovic I, Živanović J, Šošić-Jurjević B, Trifunovic S, Tanić N, Milošević V. Immuno-Histomorphometric and -Fluorescent Characteristics of Gh Cells After Treatment with Genistein Or Daidzein in An Animal Model of Andropause. in Acta Veterinaria, Beograd. 2014;64(1):93-104.
doi:10.2478/acve-2014-0010 .

Ajdžanovic, Vladimir, Medigovic, Ivana, Živanović, Jasmina, Šošić-Jurjević, Branka, Trifunovic, Svetlana, Tanić, Nasta, Milošević, Verica, "Immuno-Histomorphometric and -Fluorescent Characteristics of Gh Cells After Treatment with Genistein Or Daidzein in An Animal Model of Andropause" in Acta Veterinaria, Beograd, 64, no. 1 (2014):93-104,
https://doi.org/10.2478/acve-2014-0010 . .