TY  - JOUR
AU  - Petrović, V.
AU  - Opacic-Galic, V.
AU  - Zivkovic, S.
AU  - Nikolić, Biljana
AU  - Danilovic, V.
AU  - Miletic, V.
AU  - Jokanović, Vukoman R.
AU  - Mitić-Ćulafić, Dragana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/722
AB  - AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukeys post hoc test as well as Kruskall-Wallis test with Dunns post hoc test at =0.05. ResultsAll materials significantly reduced cell viability; especially when undiluted eluates were used (P LT 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P LT 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P GT 0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).
T2  - International Endodontic Journal
T1  - Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study
VL  - 48
IS  - 10
SP  - 966
EP  - 975
DO  - 10.1111/iej.12391
ER  - 

@article{
author = "Petrović, V. and Opacic-Galic, V. and Zivkovic, S. and Nikolić, Biljana and Danilovic, V. and Miletic, V. and Jokanović, Vukoman R. and Mitić-Ćulafić, Dragana",
year = "2015",
abstract = "AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukeys post hoc test as well as Kruskall-Wallis test with Dunns post hoc test at =0.05. ResultsAll materials significantly reduced cell viability; especially when undiluted eluates were used (P LT 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P LT 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P GT 0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).",
journal = "International Endodontic Journal",
title = "Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study",
volume = "48",
number = "10",
pages = "966-975",
doi = "10.1111/iej.12391"
}

Petrović, V., Opacic-Galic, V., Zivkovic, S., Nikolić, B., Danilovic, V., Miletic, V., Jokanović, V. R.,& Mitić-Ćulafić, D.. (2015). Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. in International Endodontic Journal, 48(10), 966-975.
https://doi.org/10.1111/iej.12391

Petrović V, Opacic-Galic V, Zivkovic S, Nikolić B, Danilovic V, Miletic V, Jokanović VR, Mitić-Ćulafić D. Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. in International Endodontic Journal. 2015;48(10):966-975.
doi:10.1111/iej.12391 .

Petrović, V., Opacic-Galic, V., Zivkovic, S., Nikolić, Biljana, Danilovic, V., Miletic, V., Jokanović, Vukoman R., Mitić-Ćulafić, Dragana, "Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study" in International Endodontic Journal, 48, no. 10 (2015):966-975,
https://doi.org/10.1111/iej.12391 . .

TY  - JOUR
AU  - Opacic-Galic, V.
AU  - Petrovic, V.
AU  - Zivkovic, S.
AU  - Jokanović, Vukoman R.
AU  - Nikolić, Biljana
AU  - Knežević-Vukčević, Jelena
AU  - Mitić-Ćulafić, Dragana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5501
AB  - Aim To characterize and investigate the genotoxic effect of a new endodontic cement based on dicalcium- and tricalcium-silicate (CS) with hydroxyapatite (HA) on human lymphocytes. Methodology Hydrothermal treatment was applied for synthesis of CS and HA. The final mixture HA-CS, with potential to be used in endodontic practice, is composed of CS (34%) and HA (66%). Human lymphocytes were incubated with HA, HA-CS and CS for 1h, at 37 degrees C and 5% CO2. Cell viability was determined using the trypan blue exclusion assay. To evaluate the level of DNA damage comet assay (single cell gel electrophoresis) was performed. For the statistical analysis anova and Duncans Post Hoc Test were used. Results The SEM analysis indicated that CS consisted mostly of agglomerates of several micrometers in size, built up from smaller particles, with dimensions between 117 and 477nm. This is promising because dimensions of agglomerates are not comparable with channels inside the cell membranes, whereas their nano-elements provide evident activity, important for faster setting of these mixtures compared to MTA. Values of DNA damage obtained in the comet assay indicated low genotoxic risk of the new endodontic materials. Conclusions The significantly improved setting characteristics and low genotoxic risk of the new material support further research.
T2  - International Endodontic Journal
T1  - New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes
VL  - 46
IS  - 6
SP  - 506
EP  - 516
DO  - 10.1111/iej.12017
ER  - 

@article{
author = "Opacic-Galic, V. and Petrovic, V. and Zivkovic, S. and Jokanović, Vukoman R. and Nikolić, Biljana and Knežević-Vukčević, Jelena and Mitić-Ćulafić, Dragana",
year = "2013",
abstract = "Aim To characterize and investigate the genotoxic effect of a new endodontic cement based on dicalcium- and tricalcium-silicate (CS) with hydroxyapatite (HA) on human lymphocytes. Methodology Hydrothermal treatment was applied for synthesis of CS and HA. The final mixture HA-CS, with potential to be used in endodontic practice, is composed of CS (34%) and HA (66%). Human lymphocytes were incubated with HA, HA-CS and CS for 1h, at 37 degrees C and 5% CO2. Cell viability was determined using the trypan blue exclusion assay. To evaluate the level of DNA damage comet assay (single cell gel electrophoresis) was performed. For the statistical analysis anova and Duncans Post Hoc Test were used. Results The SEM analysis indicated that CS consisted mostly of agglomerates of several micrometers in size, built up from smaller particles, with dimensions between 117 and 477nm. This is promising because dimensions of agglomerates are not comparable with channels inside the cell membranes, whereas their nano-elements provide evident activity, important for faster setting of these mixtures compared to MTA. Values of DNA damage obtained in the comet assay indicated low genotoxic risk of the new endodontic materials. Conclusions The significantly improved setting characteristics and low genotoxic risk of the new material support further research.",
journal = "International Endodontic Journal",
title = "New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes",
volume = "46",
number = "6",
pages = "506-516",
doi = "10.1111/iej.12017"
}

Opacic-Galic, V., Petrovic, V., Zivkovic, S., Jokanović, V. R., Nikolić, B., Knežević-Vukčević, J.,& Mitić-Ćulafić, D.. (2013). New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes. in International Endodontic Journal, 46(6), 506-516.
https://doi.org/10.1111/iej.12017

Opacic-Galic V, Petrovic V, Zivkovic S, Jokanović VR, Nikolić B, Knežević-Vukčević J, Mitić-Ćulafić D. New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes. in International Endodontic Journal. 2013;46(6):506-516.
doi:10.1111/iej.12017 .

Opacic-Galic, V., Petrovic, V., Zivkovic, S., Jokanović, Vukoman R., Nikolić, Biljana, Knežević-Vukčević, Jelena, Mitić-Ćulafić, Dragana, "New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes" in International Endodontic Journal, 46, no. 6 (2013):506-516,
https://doi.org/10.1111/iej.12017 . .

TY  - JOUR
AU  - Filipović, Suzana
AU  - Obradović, Nina
AU  - Pavlović, Vladimir B.
AU  - Petrović, V.
AU  - Mitrić, Miodrag
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3257
AB  - The aim of this work was to analyze the influence of mechanical activation on the MgCO3-TiO2 system. Mixtures of MgCO3-TiO2 were mechanically activated for 15, 30, 60 and 120 minutes in a planetary ball mill and after that sintered at 1100 degrees C for 1h. XRD analyses were performed in order to give information about the phase composition and to determine a variety of microstructure parameters using Scherrers method. Also, the effect of tribophysical activation and sintering process on microstructure was investigated by scanning electron microscopy. Electrical measurements were performed in order to determine electrical properties of sintered samples. Our conclusions are that the sample activated for 120 min showed the best electrical properties (epsilon(r)=23.86, Q=233, rho=0.38) and exhibited the best sinterability.
T2  - Science of Sintering
T1  - Influence of Mechanical Activation on Structural and Electrical Properties of Sintered MgTiO3 Ceramics
VL  - 41
IS  - 2
SP  - 117
EP  - 123
DO  - 10.2298/SOS0902117F
ER  - 

@article{
author = "Filipović, Suzana and Obradović, Nina and Pavlović, Vladimir B. and Petrović, V. and Mitrić, Miodrag",
year = "2009",
abstract = "The aim of this work was to analyze the influence of mechanical activation on the MgCO3-TiO2 system. Mixtures of MgCO3-TiO2 were mechanically activated for 15, 30, 60 and 120 minutes in a planetary ball mill and after that sintered at 1100 degrees C for 1h. XRD analyses were performed in order to give information about the phase composition and to determine a variety of microstructure parameters using Scherrers method. Also, the effect of tribophysical activation and sintering process on microstructure was investigated by scanning electron microscopy. Electrical measurements were performed in order to determine electrical properties of sintered samples. Our conclusions are that the sample activated for 120 min showed the best electrical properties (epsilon(r)=23.86, Q=233, rho=0.38) and exhibited the best sinterability.",
journal = "Science of Sintering",
title = "Influence of Mechanical Activation on Structural and Electrical Properties of Sintered MgTiO3 Ceramics",
volume = "41",
number = "2",
pages = "117-123",
doi = "10.2298/SOS0902117F"
}

Filipović, S., Obradović, N., Pavlović, V. B., Petrović, V.,& Mitrić, M.. (2009). Influence of Mechanical Activation on Structural and Electrical Properties of Sintered MgTiO3 Ceramics. in Science of Sintering, 41(2), 117-123.
https://doi.org/10.2298/SOS0902117F

Filipović S, Obradović N, Pavlović VB, Petrović V, Mitrić M. Influence of Mechanical Activation on Structural and Electrical Properties of Sintered MgTiO3 Ceramics. in Science of Sintering. 2009;41(2):117-123.
doi:10.2298/SOS0902117F .

Filipović, Suzana, Obradović, Nina, Pavlović, Vladimir B., Petrović, V., Mitrić, Miodrag, "Influence of Mechanical Activation on Structural and Electrical Properties of Sintered MgTiO3 Ceramics" in Science of Sintering, 41, no. 2 (2009):117-123,
https://doi.org/10.2298/SOS0902117F . .