TY  - JOUR
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Guć-Šćekić, Marija
AU  - Vujić, Dragana
AU  - Joksić, Gordana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5986
AB  - Purpose: As the Fanconi anemia (FA) pathway is required for appropriate cell cycle progression through mitosis and the completion of cell division, the aim of the present study was to determine the destiny of FA cells after irradiation in vitro and to elucidate any difference in radiosensitivity between FA and control cells. Materials and methods: Analyses of phosphorylated histone H2AX (gamma-H2AX) foci, micronuclei formation and cell cycle analysis were performed in unirradiated (0 min) and irradiated primary FA fibroblasts and in a control group at different post-irradiation times (30 min, 2 h, 5 h and 24 h). Results: The accumulation of gamma-H2AX foci in irradiated FA fibroblasts was observed. At 24 h post-irradiation, 57% of FA cells were gamma-H2AX foci-positive, significantly higher than in the control (p LT 0.01). The cell cycle analysis has shown the transient G2/M arrest in irradiated FA fibroblasts. The portion of cells in the G2/M phase showed initial increase at 30 min post-irradiation and afterwards decreased over time reaching the pretreatment level 24 h after irradiation. Irradiated FA fibroblasts progressed to abnormal mitosis, as is shown by the production of cells with different nuclear morphologies from binucleated to multinucleated surrounded with micronuclei, and also by a high percentage of foci-positive micronuclei. The majority of radiation-induced micronuclei were gamma-H2AX foci-positive, indicating that radiation-induced micronuclei contain fragments of damaged chromosomes. In contrast, in the control group, most of the micronuclei were classified as gamma-H2AX foci-negative, which indicates that cells with unrepaired damage were blocked before entering mitosis. Conclusion: The results clearly indicate that mitotic catastrophe might be an important cell-death mechanism involved in the response of FA fibroblasts to ionizing radiation.
T2  - International Journal of Radiation Biology
T1  - Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts
VL  - 90
IS  - 5
SP  - 373
EP  - 381
DO  - 10.3109/09553002.2014.892224
ER  - 

@article{
author = "Leskovac, Andreja and Petrović, Sandra and Guć-Šćekić, Marija and Vujić, Dragana and Joksić, Gordana",
year = "2014",
abstract = "Purpose: As the Fanconi anemia (FA) pathway is required for appropriate cell cycle progression through mitosis and the completion of cell division, the aim of the present study was to determine the destiny of FA cells after irradiation in vitro and to elucidate any difference in radiosensitivity between FA and control cells. Materials and methods: Analyses of phosphorylated histone H2AX (gamma-H2AX) foci, micronuclei formation and cell cycle analysis were performed in unirradiated (0 min) and irradiated primary FA fibroblasts and in a control group at different post-irradiation times (30 min, 2 h, 5 h and 24 h). Results: The accumulation of gamma-H2AX foci in irradiated FA fibroblasts was observed. At 24 h post-irradiation, 57% of FA cells were gamma-H2AX foci-positive, significantly higher than in the control (p LT 0.01). The cell cycle analysis has shown the transient G2/M arrest in irradiated FA fibroblasts. The portion of cells in the G2/M phase showed initial increase at 30 min post-irradiation and afterwards decreased over time reaching the pretreatment level 24 h after irradiation. Irradiated FA fibroblasts progressed to abnormal mitosis, as is shown by the production of cells with different nuclear morphologies from binucleated to multinucleated surrounded with micronuclei, and also by a high percentage of foci-positive micronuclei. The majority of radiation-induced micronuclei were gamma-H2AX foci-positive, indicating that radiation-induced micronuclei contain fragments of damaged chromosomes. In contrast, in the control group, most of the micronuclei were classified as gamma-H2AX foci-negative, which indicates that cells with unrepaired damage were blocked before entering mitosis. Conclusion: The results clearly indicate that mitotic catastrophe might be an important cell-death mechanism involved in the response of FA fibroblasts to ionizing radiation.",
journal = "International Journal of Radiation Biology",
title = "Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts",
volume = "90",
number = "5",
pages = "373-381",
doi = "10.3109/09553002.2014.892224"
}

Leskovac, A., Petrović, S., Guć-Šćekić, M., Vujić, D.,& Joksić, G.. (2014). Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts. in International Journal of Radiation Biology, 90(5), 373-381.
https://doi.org/10.3109/09553002.2014.892224

Leskovac A, Petrović S, Guć-Šćekić M, Vujić D, Joksić G. Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts. in International Journal of Radiation Biology. 2014;90(5):373-381.
doi:10.3109/09553002.2014.892224 .

Leskovac, Andreja, Petrović, Sandra, Guć-Šćekić, Marija, Vujić, Dragana, Joksić, Gordana, "Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts" in International Journal of Radiation Biology, 90, no. 5 (2014):373-381,
https://doi.org/10.3109/09553002.2014.892224 . .

TY  - JOUR
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Joksić, Ivana
AU  - Filipović, Jelena G.
AU  - Vujić, Dragana
AU  - Guć-Šćekić, Marija
AU  - Joksić, Gordana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5606
AB  - Fanconi anemia (FA) is a rare cancer-prone genetic disease characterized by impaired oxygen metabolism and defects in DNA damage repair. Response of FA cells to ionizing radiation has been an issue intensively debated in the literature. To study in vitro radiosensitivity in patients suffering from FA and their parents (heterozygous carriers), we determined radiation-induced leukocyte apoptosis using flow cytometry. As TP53 gene is involved in the control of apoptosis, we studied its status in FA lymphocytes using dual colour fluorescence in situ hybridization (FISH). FA patients and female heterozygous carriers display radiosensitive response to ionizing radiation seen as abnormal elimination of cells via apoptosis. By employment of FISH, the TP53 allele loss in FA lymphocytes was not observed. In diseases related to oxidative stress, determination of radiation-induced apoptosis is the method of choice for testing the radiosensitivity.
T2  - Current Science
T1  - Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia
VL  - 105
IS  - 1
SP  - 56
EP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5606
ER  - 

@article{
author = "Petrović, Sandra and Leskovac, Andreja and Joksić, Ivana and Filipović, Jelena G. and Vujić, Dragana and Guć-Šćekić, Marija and Joksić, Gordana",
year = "2013",
abstract = "Fanconi anemia (FA) is a rare cancer-prone genetic disease characterized by impaired oxygen metabolism and defects in DNA damage repair. Response of FA cells to ionizing radiation has been an issue intensively debated in the literature. To study in vitro radiosensitivity in patients suffering from FA and their parents (heterozygous carriers), we determined radiation-induced leukocyte apoptosis using flow cytometry. As TP53 gene is involved in the control of apoptosis, we studied its status in FA lymphocytes using dual colour fluorescence in situ hybridization (FISH). FA patients and female heterozygous carriers display radiosensitive response to ionizing radiation seen as abnormal elimination of cells via apoptosis. By employment of FISH, the TP53 allele loss in FA lymphocytes was not observed. In diseases related to oxidative stress, determination of radiation-induced apoptosis is the method of choice for testing the radiosensitivity.",
journal = "Current Science",
title = "Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia",
volume = "105",
number = "1",
pages = "56-60",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5606"
}

Petrović, S., Leskovac, A., Joksić, I., Filipović, J. G., Vujić, D., Guć-Šćekić, M.,& Joksić, G.. (2013). Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia. in Current Science, 105(1), 56-60.
https://hdl.handle.net/21.15107/rcub_vinar_5606

Petrović S, Leskovac A, Joksić I, Filipović JG, Vujić D, Guć-Šćekić M, Joksić G. Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia. in Current Science. 2013;105(1):56-60.
https://hdl.handle.net/21.15107/rcub_vinar_5606 .

Petrović, Sandra, Leskovac, Andreja, Joksić, Ivana, Filipović, Jelena G., Vujić, Dragana, Guć-Šćekić, Marija, Joksić, Gordana, "Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia" in Current Science, 105, no. 1 (2013):56-60,
https://hdl.handle.net/21.15107/rcub_vinar_5606 .

TY  - JOUR
AU  - Joksić, Ivana
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Filipović, Jelena G.
AU  - Guć-Šćekić, Marija
AU  - Vujić, Dragana
AU  - Joksić, Gordana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5798
AB  - Fanconi anemia (FA) is a rare genetically heterogeneous disease characterized by developmental abnormalities, progressive bone marrow failure, and cancer susceptibility. We examined spontaneous, diepoxybutane (DEB)-induced and radiation-induced sister chromatid exchanges (SCEs) in wholeblood lymphocyte cultures of bone marrow failure (BMF) patients including Fanconi anemia, mothers of affected individuals, and healthy controls. The baseline frequency of SCE in FA cells was similar to that observed in controls. However, in response to DEB SCE frequencies in FA patients and their mothers were significantly increased compared to both non-FA BMF families and healthy controls. In response to ionizing radiation, cells displayed increased frequency of SCE, but no differences between FA patients and non-FA BMF patients were seen. Our data confirm and expand previous findings by showing that SCE induced by DEB can be used as an adjunct diagnostic test not only for FA patients, but also for female heterozygous carriers, at least for complementation groups FANCA and FANCD2.
T2  - Genetika
T1  - Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype
VL  - 45
IS  - 2
SP  - 393
EP  - 403
DO  - 10.2298/GENSR1302393J
ER  - 

@article{
author = "Joksić, Ivana and Petrović, Sandra and Leskovac, Andreja and Filipović, Jelena G. and Guć-Šćekić, Marija and Vujić, Dragana and Joksić, Gordana",
year = "2013",
abstract = "Fanconi anemia (FA) is a rare genetically heterogeneous disease characterized by developmental abnormalities, progressive bone marrow failure, and cancer susceptibility. We examined spontaneous, diepoxybutane (DEB)-induced and radiation-induced sister chromatid exchanges (SCEs) in wholeblood lymphocyte cultures of bone marrow failure (BMF) patients including Fanconi anemia, mothers of affected individuals, and healthy controls. The baseline frequency of SCE in FA cells was similar to that observed in controls. However, in response to DEB SCE frequencies in FA patients and their mothers were significantly increased compared to both non-FA BMF families and healthy controls. In response to ionizing radiation, cells displayed increased frequency of SCE, but no differences between FA patients and non-FA BMF patients were seen. Our data confirm and expand previous findings by showing that SCE induced by DEB can be used as an adjunct diagnostic test not only for FA patients, but also for female heterozygous carriers, at least for complementation groups FANCA and FANCD2.",
journal = "Genetika",
title = "Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype",
volume = "45",
number = "2",
pages = "393-403",
doi = "10.2298/GENSR1302393J"
}

Joksić, I., Petrović, S., Leskovac, A., Filipović, J. G., Guć-Šćekić, M., Vujić, D.,& Joksić, G.. (2013). Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype. in Genetika, 45(2), 393-403.
https://doi.org/10.2298/GENSR1302393J

Joksić I, Petrović S, Leskovac A, Filipović JG, Guć-Šćekić M, Vujić D, Joksić G. Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype. in Genetika. 2013;45(2):393-403.
doi:10.2298/GENSR1302393J .

Joksić, Ivana, Petrović, Sandra, Leskovac, Andreja, Filipović, Jelena G., Guć-Šćekić, Marija, Vujić, Dragana, Joksić, Gordana, "Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype" in Genetika, 45, no. 2 (2013):393-403,
https://doi.org/10.2298/GENSR1302393J . .

TY  - JOUR
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Kotur-Stevuljevic, Jelena
AU  - Joksić, Jelena
AU  - Guć-Šćekić, Marija
AU  - Vujić, Dragana
AU  - Joksić, Gordana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4379
AB  - Fanconi anemia (FA) is a rare cancer-prone genetic disorder characterized by progressive bone marrow failure, chromosomal instability and redox abnormalities. There is much biochemical and genetic data, which strongly suggest that FA cells experience increased oxidative stress. The present study was designed to elucidate if differences in oxidant state exist between control, idiopathic bone marrow failure (idBMF) and FA cells, and to analyze oxidant state of cells in FA heterozygous carriers as well. The results of the present study confirm an in vivo prooxidant state of FA cells and clearly indicate that FA patients can be distinguished from idBMF patients based on the oxidant state of cells. Female carriers of FA mutation also exhibited hallmarks of an in vivo prooxidant state behaving in a similar manner as FA patients. On the other hand, the oxidant state of cells in FA male carriers and idBMF families failed to show any significant difference vs. controls. We demonstrate that the altered oxidant state influences susceptibility of cells to apoptosis in both FA patients and female carriers. The results highlight the need for further research of the possible role of mitochondrial inheritance in the pathogenesis of FA.
T2  - Biological Chemistry
T1  - Gender-related differences in the oxidant state of cells in Fanconi anemia heterozygotes
VL  - 392
IS  - 7
SP  - 625
EP  - 632
DO  - 10.1515/BC.2011.064
ER  - 

@article{
author = "Petrović, Sandra and Leskovac, Andreja and Kotur-Stevuljevic, Jelena and Joksić, Jelena and Guć-Šćekić, Marija and Vujić, Dragana and Joksić, Gordana",
year = "2011",
abstract = "Fanconi anemia (FA) is a rare cancer-prone genetic disorder characterized by progressive bone marrow failure, chromosomal instability and redox abnormalities. There is much biochemical and genetic data, which strongly suggest that FA cells experience increased oxidative stress. The present study was designed to elucidate if differences in oxidant state exist between control, idiopathic bone marrow failure (idBMF) and FA cells, and to analyze oxidant state of cells in FA heterozygous carriers as well. The results of the present study confirm an in vivo prooxidant state of FA cells and clearly indicate that FA patients can be distinguished from idBMF patients based on the oxidant state of cells. Female carriers of FA mutation also exhibited hallmarks of an in vivo prooxidant state behaving in a similar manner as FA patients. On the other hand, the oxidant state of cells in FA male carriers and idBMF families failed to show any significant difference vs. controls. We demonstrate that the altered oxidant state influences susceptibility of cells to apoptosis in both FA patients and female carriers. The results highlight the need for further research of the possible role of mitochondrial inheritance in the pathogenesis of FA.",
journal = "Biological Chemistry",
title = "Gender-related differences in the oxidant state of cells in Fanconi anemia heterozygotes",
volume = "392",
number = "7",
pages = "625-632",
doi = "10.1515/BC.2011.064"
}

Petrović, S., Leskovac, A., Kotur-Stevuljevic, J., Joksić, J., Guć-Šćekić, M., Vujić, D.,& Joksić, G.. (2011). Gender-related differences in the oxidant state of cells in Fanconi anemia heterozygotes. in Biological Chemistry, 392(7), 625-632.
https://doi.org/10.1515/BC.2011.064

Petrović S, Leskovac A, Kotur-Stevuljevic J, Joksić J, Guć-Šćekić M, Vujić D, Joksić G. Gender-related differences in the oxidant state of cells in Fanconi anemia heterozygotes. in Biological Chemistry. 2011;392(7):625-632.
doi:10.1515/BC.2011.064 .

Petrović, Sandra, Leskovac, Andreja, Kotur-Stevuljevic, Jelena, Joksić, Jelena, Guć-Šćekić, Marija, Vujić, Dragana, Joksić, Gordana, "Gender-related differences in the oxidant state of cells in Fanconi anemia heterozygotes" in Biological Chemistry, 392, no. 7 (2011):625-632,
https://doi.org/10.1515/BC.2011.064 . .

TY  - CONF
AU  - Guć-Šćekić, Marija
AU  - Milenkovic, Tanja
AU  - Zdravkovic, Dragan
AU  - Topic, Vesna
AU  - Liehr, Thomas
AU  - Joksić, Gordana
AU  - Radivojević, Danijela
AU  - Lakić, Nina
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1756
C3  - Chromosome Research
T1  - Classic and molecular cytogenetic findings in a 10-year-old boy with ring Y chromosome mosaicism: a case report
VL  - 19
SP  - S38
EP  - S39
DO  - 10.1007/s10577-011-9215-6
ER  - 

@conference{
author = "Guć-Šćekić, Marija and Milenkovic, Tanja and Zdravkovic, Dragan and Topic, Vesna and Liehr, Thomas and Joksić, Gordana and Radivojević, Danijela and Lakić, Nina",
year = "2011",
journal = "Chromosome Research",
title = "Classic and molecular cytogenetic findings in a 10-year-old boy with ring Y chromosome mosaicism: a case report",
volume = "19",
pages = "S38-S39",
doi = "10.1007/s10577-011-9215-6"
}

Guć-Šćekić, M., Milenkovic, T., Zdravkovic, D., Topic, V., Liehr, T., Joksić, G., Radivojević, D.,& Lakić, N.. (2011). Classic and molecular cytogenetic findings in a 10-year-old boy with ring Y chromosome mosaicism: a case report. in Chromosome Research, 19, S38-S39.
https://doi.org/10.1007/s10577-011-9215-6

Guć-Šćekić M, Milenkovic T, Zdravkovic D, Topic V, Liehr T, Joksić G, Radivojević D, Lakić N. Classic and molecular cytogenetic findings in a 10-year-old boy with ring Y chromosome mosaicism: a case report. in Chromosome Research. 2011;19:S38-S39.
doi:10.1007/s10577-011-9215-6 .

Guć-Šćekić, Marija, Milenkovic, Tanja, Zdravkovic, Dragan, Topic, Vesna, Liehr, Thomas, Joksić, Gordana, Radivojević, Danijela, Lakić, Nina, "Classic and molecular cytogenetic findings in a 10-year-old boy with ring Y chromosome mosaicism: a case report" in Chromosome Research, 19 (2011):S38-S39,
https://doi.org/10.1007/s10577-011-9215-6 . .

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Vujić, Dragana
AU  - Guć-Šćekić, Marija
AU  - Petrović, Sandra
AU  - Joksić, Ivana
AU  - Slijepcevic, Predrag
AU  - Joksić, Gordana
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4000
AB  - Among patients with bone marrow failure (BMF) syndrome, some are happened to have underlying Fanconi anemia (FA), a genetically heterogeneous disease, which is characterized by progressive pancytopenia and cancer susceptibility. Due to heterogeneous nature of the disease, a single genetic test, as in vitro response to DNA cross-linking agents, usually is not enough to make correct diagnosis. The aim of this study was to evaluate whether measuring repair kinetics of radiation-induced DNA double-strand breaks (DSBs) can distinguish Fanconi anemia from other BMF patients. An early step in repair of DSBs is phosphorylation of the histone H2AX, generating gamma-H2AX histone, which extends over mega base-pair regions of DNA from the break site and is visualised as foci (gamma-H2AX foci) with specific antibodies. The primary fibroblasts, established from FA patients, were exposed to gamma-rays, a dose of 2 Gy (Co-60), incubated for up to 24 hours under repair-permissive conditions, and assayed for the level of gamma-H2AX foci and apoptosis at different recovery times after the treatment. Cell lines originating from FA patients displayed a significant delay in the repair of radiation-induced DNA DSBs relative to non-FA bone marrow failure (non-FA BMF) and control cell lines. The delay is especially evident at recovery time of 24 hours, and is seen as about 8-fold increase of residual gamma-H2AX foci compared to self-state before irradiation. The delay in repair kinetics of FA cells represents the unique feature of FA cellular phenotype, which should be exploited to distinguish FA cellular phenotype.
T2  - Tohoku Journal of Experimental Medicine
T1  - Fanconi Anemia Is Characterized by Delayed Repair Kinetics of DNA Double-Strand Breaks
VL  - 221
IS  - 1
SP  - 69
EP  - 76
DO  - 10.1620/tjem.221.69
ER  - 

@article{
author = "Leskovac, Andreja and Vujić, Dragana and Guć-Šćekić, Marija and Petrović, Sandra and Joksić, Ivana and Slijepcevic, Predrag and Joksić, Gordana",
year = "2010",
abstract = "Among patients with bone marrow failure (BMF) syndrome, some are happened to have underlying Fanconi anemia (FA), a genetically heterogeneous disease, which is characterized by progressive pancytopenia and cancer susceptibility. Due to heterogeneous nature of the disease, a single genetic test, as in vitro response to DNA cross-linking agents, usually is not enough to make correct diagnosis. The aim of this study was to evaluate whether measuring repair kinetics of radiation-induced DNA double-strand breaks (DSBs) can distinguish Fanconi anemia from other BMF patients. An early step in repair of DSBs is phosphorylation of the histone H2AX, generating gamma-H2AX histone, which extends over mega base-pair regions of DNA from the break site and is visualised as foci (gamma-H2AX foci) with specific antibodies. The primary fibroblasts, established from FA patients, were exposed to gamma-rays, a dose of 2 Gy (Co-60), incubated for up to 24 hours under repair-permissive conditions, and assayed for the level of gamma-H2AX foci and apoptosis at different recovery times after the treatment. Cell lines originating from FA patients displayed a significant delay in the repair of radiation-induced DNA DSBs relative to non-FA bone marrow failure (non-FA BMF) and control cell lines. The delay is especially evident at recovery time of 24 hours, and is seen as about 8-fold increase of residual gamma-H2AX foci compared to self-state before irradiation. The delay in repair kinetics of FA cells represents the unique feature of FA cellular phenotype, which should be exploited to distinguish FA cellular phenotype.",
journal = "Tohoku Journal of Experimental Medicine",
title = "Fanconi Anemia Is Characterized by Delayed Repair Kinetics of DNA Double-Strand Breaks",
volume = "221",
number = "1",
pages = "69-76",
doi = "10.1620/tjem.221.69"
}

Leskovac, A., Vujić, D., Guć-Šćekić, M., Petrović, S., Joksić, I., Slijepcevic, P.,& Joksić, G.. (2010). Fanconi Anemia Is Characterized by Delayed Repair Kinetics of DNA Double-Strand Breaks. in Tohoku Journal of Experimental Medicine, 221(1), 69-76.
https://doi.org/10.1620/tjem.221.69

Leskovac A, Vujić D, Guć-Šćekić M, Petrović S, Joksić I, Slijepcevic P, Joksić G. Fanconi Anemia Is Characterized by Delayed Repair Kinetics of DNA Double-Strand Breaks. in Tohoku Journal of Experimental Medicine. 2010;221(1):69-76.
doi:10.1620/tjem.221.69 .

Leskovac, Andreja, Vujić, Dragana, Guć-Šćekić, Marija, Petrović, Sandra, Joksić, Ivana, Slijepcevic, Predrag, Joksić, Gordana, "Fanconi Anemia Is Characterized by Delayed Repair Kinetics of DNA Double-Strand Breaks" in Tohoku Journal of Experimental Medicine, 221, no. 1 (2010):69-76,
https://doi.org/10.1620/tjem.221.69 . .

TY  - CONF
AU  - Petrović, Sandra
AU  - Vujić, Dragana
AU  - Guć-Šćekić, Marija
AU  - Joksić, Ivana
AU  - Leskovac, Andreja
AU  - Joksić, Gordana
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11708
AB  - Fankonijeva anemija (FA) je genetičko oboljenje karakterisano progresivnom pancitopenijom i predispozicijom ka malignitetima. Asocirano je sa poremećajima u redoks procesima u ćeliji što ga čini i bolešću oksidativnog stresa. FA ćelije ispoljavaju veliku osetljivost na DNK klastogene agense, dok su podaci o njihovoj osetljivosti na jonizujuće zračenje kontradiktorni. Cilj ove studije je ispitivanje in vitro radioosetljivosti FA homozigota i heterozigota i utvrđivanje uticaja antioksidativnih enzima na ukupan radiobiološki odgovor ćelija. FA pacijenti ispoljavaju značajno sniženje aktivnosti katalaza, sniženu vrednost superoksid dismutaza i povećani bazalni nivo mikronukleusa u odnosu na kontrolu. Radiorezistentni odgovor na jonizujuće zračenje utvrđen je kod većine pacijenata i praćen je značajno većim procentom zračenjem indukovane apoptoze. Utvrđena je negativna korelacija između aktivnosti katalaza i procenta apoptotskih ćelija. Heterozigoti-roditelji imaju visok procenat zračenjem indukovane apoptoze koja je kod nosioca-majki praćena značajnim sniženjem aktivnosti katalaza. Rezultati pokazuju da snižena vrednost katalaza značajno doprinosi ukupnom radiobiološkom odgovoru ćelija.
PB  - Beograd : Društvo genetičara Srbije
C3  - IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara
T1  - Uticaj antioksidativniih enzima na ukupni radiobiološki odgovor limfocita pacienata obolelih od Fankonijeve anemije
SP  - 52
EP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11708
ER  - 

@conference{
author = "Petrović, Sandra and Vujić, Dragana and Guć-Šćekić, Marija and Joksić, Ivana and Leskovac, Andreja and Joksić, Gordana",
year = "2009",
abstract = "Fankonijeva anemija (FA) je genetičko oboljenje karakterisano progresivnom pancitopenijom i predispozicijom ka malignitetima. Asocirano je sa poremećajima u redoks procesima u ćeliji što ga čini i bolešću oksidativnog stresa. FA ćelije ispoljavaju veliku osetljivost na DNK klastogene agense, dok su podaci o njihovoj osetljivosti na jonizujuće zračenje kontradiktorni. Cilj ove studije je ispitivanje in vitro radioosetljivosti FA homozigota i heterozigota i utvrđivanje uticaja antioksidativnih enzima na ukupan radiobiološki odgovor ćelija. FA pacijenti ispoljavaju značajno sniženje aktivnosti katalaza, sniženu vrednost superoksid dismutaza i povećani bazalni nivo mikronukleusa u odnosu na kontrolu. Radiorezistentni odgovor na jonizujuće zračenje utvrđen je kod većine pacijenata i praćen je značajno većim procentom zračenjem indukovane apoptoze. Utvrđena je negativna korelacija između aktivnosti katalaza i procenta apoptotskih ćelija. Heterozigoti-roditelji imaju visok procenat zračenjem indukovane apoptoze koja je kod nosioca-majki praćena značajnim sniženjem aktivnosti katalaza. Rezultati pokazuju da snižena vrednost katalaza značajno doprinosi ukupnom radiobiološkom odgovoru ćelija.",
publisher = "Beograd : Društvo genetičara Srbije",
journal = "IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara",
title = "Uticaj antioksidativniih enzima na ukupni radiobiološki odgovor limfocita pacienata obolelih od Fankonijeve anemije",
pages = "52-52",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11708"
}

Petrović, S., Vujić, D., Guć-Šćekić, M., Joksić, I., Leskovac, A.,& Joksić, G.. (2009). Uticaj antioksidativniih enzima na ukupni radiobiološki odgovor limfocita pacienata obolelih od Fankonijeve anemije. in IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara
Beograd : Društvo genetičara Srbije., 52-52.
https://hdl.handle.net/21.15107/rcub_vinar_11708

Petrović S, Vujić D, Guć-Šćekić M, Joksić I, Leskovac A, Joksić G. Uticaj antioksidativniih enzima na ukupni radiobiološki odgovor limfocita pacienata obolelih od Fankonijeve anemije. in IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara. 2009;:52-52.
https://hdl.handle.net/21.15107/rcub_vinar_11708 .

Petrović, Sandra, Vujić, Dragana, Guć-Šćekić, Marija, Joksić, Ivana, Leskovac, Andreja, Joksić, Gordana, "Uticaj antioksidativniih enzima na ukupni radiobiološki odgovor limfocita pacienata obolelih od Fankonijeve anemije" in IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara (2009):52-52,
https://hdl.handle.net/21.15107/rcub_vinar_11708 .

TY  - CONF
AU  - Joksić, Ivana
AU  - Guć-Šćekić, Marija
AU  - Vujić, Dragana
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11709
AB  - Telomere su nukleoproteinski kompleksi smešteni na krajevima hromozoma koji imaju ključnu funkciju u očuvanju integriteta hromozomskih sekvenci, sprečavajući degradaciju DNK egzonukleazama i neadekvatnu aktivaciju kontrolnih tačaka ćelijskog ciklusa i puteva popravke DNK. TIF metoda (fokusi indukovani telomernom disfunkcijom), predstvalja ko-lokalizaciju faktora DNK repera (53BP1 i γH2AX) i telomera i omogućava kvantifikaciju nefunkcionalnih telomera u različitim tkivima. U primarnim ćelijskim linijama fibroblasta kože devet pacijenata sa sindromom aplazije kostne srži i fenotipom Fankonijeve anemije određivana je bazalna incidenca SCE, T-SCE, γH2AX i TIF. Prosečna učestalost SCE iznoslia je 4.42±0.96 (3.38 do 6.5) po ćeliji, dok je incidenca T-SCE iznoslia 1.91±0.81 (1 do 3.33) po ćeliji. Bazalne vrednosti γH2AX iznosile su 2.27±1.55 (0.8 do 4.2), dok je prosečna učestalosta TIF iznoslia 2.97±1.47 (od 1.44 do 5) po ćeliji. Statistički značajna pozitivna korelacija uočena je između incidence SCE i TIF (0.77 p<0.05), ukazujući da je disfunkcija telomera verovatno nastala mehanizmom homologe rekombinacije.
PB  - Beograd : Društvo genetičara Srbije
C3  - IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara
T1  - Disfunkcija telomera pozitivno koreliše sa incidencom izmena sestrinskih hromatida kod pacijenata obolelih of Fankonijeve anemije
SP  - 103
EP  - 103
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11709
ER  - 

@conference{
author = "Joksić, Ivana and Guć-Šćekić, Marija and Vujić, Dragana and Petrović, Sandra and Leskovac, Andreja",
year = "2009",
abstract = "Telomere su nukleoproteinski kompleksi smešteni na krajevima hromozoma koji imaju ključnu funkciju u očuvanju integriteta hromozomskih sekvenci, sprečavajući degradaciju DNK egzonukleazama i neadekvatnu aktivaciju kontrolnih tačaka ćelijskog ciklusa i puteva popravke DNK. TIF metoda (fokusi indukovani telomernom disfunkcijom), predstvalja ko-lokalizaciju faktora DNK repera (53BP1 i γH2AX) i telomera i omogućava kvantifikaciju nefunkcionalnih telomera u različitim tkivima. U primarnim ćelijskim linijama fibroblasta kože devet pacijenata sa sindromom aplazije kostne srži i fenotipom Fankonijeve anemije određivana je bazalna incidenca SCE, T-SCE, γH2AX i TIF. Prosečna učestalost SCE iznoslia je 4.42±0.96 (3.38 do 6.5) po ćeliji, dok je incidenca T-SCE iznoslia 1.91±0.81 (1 do 3.33) po ćeliji. Bazalne vrednosti γH2AX iznosile su 2.27±1.55 (0.8 do 4.2), dok je prosečna učestalosta TIF iznoslia 2.97±1.47 (od 1.44 do 5) po ćeliji. Statistički značajna pozitivna korelacija uočena je između incidence SCE i TIF (0.77 p<0.05), ukazujući da je disfunkcija telomera verovatno nastala mehanizmom homologe rekombinacije.",
publisher = "Beograd : Društvo genetičara Srbije",
journal = "IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara",
title = "Disfunkcija telomera pozitivno koreliše sa incidencom izmena sestrinskih hromatida kod pacijenata obolelih of Fankonijeve anemije",
pages = "103-103",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11709"
}

Joksić, I., Guć-Šćekić, M., Vujić, D., Petrović, S.,& Leskovac, A.. (2009). Disfunkcija telomera pozitivno koreliše sa incidencom izmena sestrinskih hromatida kod pacijenata obolelih of Fankonijeve anemije. in IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara
Beograd : Društvo genetičara Srbije., 103-103.
https://hdl.handle.net/21.15107/rcub_vinar_11709

Joksić I, Guć-Šćekić M, Vujić D, Petrović S, Leskovac A. Disfunkcija telomera pozitivno koreliše sa incidencom izmena sestrinskih hromatida kod pacijenata obolelih of Fankonijeve anemije. in IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara. 2009;:103-103.
https://hdl.handle.net/21.15107/rcub_vinar_11709 .

Joksić, Ivana, Guć-Šćekić, Marija, Vujić, Dragana, Petrović, Sandra, Leskovac, Andreja, "Disfunkcija telomera pozitivno koreliše sa incidencom izmena sestrinskih hromatida kod pacijenata obolelih of Fankonijeve anemije" in IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara (2009):103-103,
https://hdl.handle.net/21.15107/rcub_vinar_11709 .

TY  - CONF
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Joksić, Ivana
AU  - Vujić, Dragana
AU  - Guć-Šćekić, Marija
AU  - Slijepčević, Predrag
AU  - Joksić, Gordana
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11710
AB  - Ćelije obolelih od Fankonijeve anemije pokazuju preosetljivost na DNK-unakrsno vezujuće agense, ali je njihov odgovor na jonizujuće zračenje još uvek nedovoljno proučen. Cilj ove studije bio je ispitati radioosetljivost fibroblasta obolelih od aplastične (AA) i fankonijeve (FA) anemije in vitro, praćenjem fosforilisanog histona γH2AX i procenta ćelija u apoptozi. Imunofluorescentnom metodom određeni γH2AX fokusi korišćeni su za kvantifikaciju dvolančanih prekida. Najveći broj γH2AX fokusa u FA ćelijama utvrđen je 30 min, a u AA ćelijama 24 h nakon ozračivanja. U kontrolnim ćelijama najveći broj apoptotičnih ćelija utvrđen je 30 min i 24 h, a kod obolelih od FA i AA, 2 i 24 h nakon ozračivanja. Odogođena apoptoza i zastoj u ćelijskom ciklusu u FA i AA ćelijama nakon ozračivanja ukazuje na poremećaj u radiobiološkom odgovoru, dok merenje dvolančanih prekida preko γH2AX 30 min nakon ozračivanja može biti značajan parametar za diferencijalnu dijagnostiku FA i AA ćelijskog fenotipa. Ovo istraživanje je pokazalo da praćenje γH2AX fokusa predstavlja veoma osetljivu metodu značajnu za procenu genomske radioosetljivosti, kao i da postoji jasna razlika u ponašanju FA, AA i normalnih fibroblasta u odgovoru na jonizujuće zračenje.
PB  - Beograd : Društvo genetičara Srbije
C3  - IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara
T1  - Radioosetljivost fibroblasta pacijenata obolelih of Fankonijeve anemije
SP  - 53
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11710
ER  - 

@conference{
author = "Leskovac, Andreja and Petrović, Sandra and Joksić, Ivana and Vujić, Dragana and Guć-Šćekić, Marija and Slijepčević, Predrag and Joksić, Gordana",
year = "2009",
abstract = "Ćelije obolelih od Fankonijeve anemije pokazuju preosetljivost na DNK-unakrsno vezujuće agense, ali je njihov odgovor na jonizujuće zračenje još uvek nedovoljno proučen. Cilj ove studije bio je ispitati radioosetljivost fibroblasta obolelih od aplastične (AA) i fankonijeve (FA) anemije in vitro, praćenjem fosforilisanog histona γH2AX i procenta ćelija u apoptozi. Imunofluorescentnom metodom određeni γH2AX fokusi korišćeni su za kvantifikaciju dvolančanih prekida. Najveći broj γH2AX fokusa u FA ćelijama utvrđen je 30 min, a u AA ćelijama 24 h nakon ozračivanja. U kontrolnim ćelijama najveći broj apoptotičnih ćelija utvrđen je 30 min i 24 h, a kod obolelih od FA i AA, 2 i 24 h nakon ozračivanja. Odogođena apoptoza i zastoj u ćelijskom ciklusu u FA i AA ćelijama nakon ozračivanja ukazuje na poremećaj u radiobiološkom odgovoru, dok merenje dvolančanih prekida preko γH2AX 30 min nakon ozračivanja može biti značajan parametar za diferencijalnu dijagnostiku FA i AA ćelijskog fenotipa. Ovo istraživanje je pokazalo da praćenje γH2AX fokusa predstavlja veoma osetljivu metodu značajnu za procenu genomske radioosetljivosti, kao i da postoji jasna razlika u ponašanju FA, AA i normalnih fibroblasta u odgovoru na jonizujuće zračenje.",
publisher = "Beograd : Društvo genetičara Srbije",
journal = "IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara",
title = "Radioosetljivost fibroblasta pacijenata obolelih of Fankonijeve anemije",
pages = "53-53",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11710"
}

Leskovac, A., Petrović, S., Joksić, I., Vujić, D., Guć-Šćekić, M., Slijepčević, P.,& Joksić, G.. (2009). Radioosetljivost fibroblasta pacijenata obolelih of Fankonijeve anemije. in IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara
Beograd : Društvo genetičara Srbije., 53-53.
https://hdl.handle.net/21.15107/rcub_vinar_11710

Leskovac A, Petrović S, Joksić I, Vujić D, Guć-Šćekić M, Slijepčević P, Joksić G. Radioosetljivost fibroblasta pacijenata obolelih of Fankonijeve anemije. in IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara. 2009;:53-53.
https://hdl.handle.net/21.15107/rcub_vinar_11710 .

Leskovac, Andreja, Petrović, Sandra, Joksić, Ivana, Vujić, Dragana, Guć-Šćekić, Marija, Slijepčević, Predrag, Joksić, Gordana, "Radioosetljivost fibroblasta pacijenata obolelih of Fankonijeve anemije" in IV Kongres genetičara Srbije : zbornik abstrakata; Jun 1-5, Tara (2009):53-53,
https://hdl.handle.net/21.15107/rcub_vinar_11710 .

TY  - CONF
AU  - Joksić, I.
AU  - Vujić, Dragana
AU  - Guć-Šćekić, Marija
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Miković, Z.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11711
AB  - Telomeres consist of repeated DNA sequences and associated proteins that lie at the termini of linear chromosomes and are critical to the protection and stability of internal chromosome sequences, a function known as telomere “capping”. In capping chromosomes ends, telomeres restrict chromosome end resection by exonucleases and prevent the improper activation of checkpoint response factors and DNA damage response pathways such as homologous recombination (HR) and non-homologous end joining (NHEJ). Telomeres shorten as cell divide, but also due to action of restriction exonucleases, oxidative damage and inappropriate recombination. When telomere shorten to a critical length, they become uncapped, which can lead to permanent cell cycle arrest. The development of the “TIF” (telomere dysfunction induced foci) assay that is based on colocalization of the DNA repair factors 53BP1 and γH2AX with uncapped telomeres enables measurement of telomere uncapping in different tissues. Our aim was to investigate correlation between homologous DNA recombination processes and function of telomeres. In nine primary fibroblast cell lines, obtained from patients presenting with bone marrow failure syndrome and Fanconi anemia clinical and cellular (diepoxybutane- DEB positive) phenotype, baseline incidence of SCE, T-SCE, γH2AX and TIF were examined. The average incidence of SCE was 4.42+-0.96, ranging from 3.38 to 6.5 per cell, whereas incidence of T-SCE was 1.91+-0.81 ranging from 1 to 3.33. Baseline level of γH2AX foci was 2.27+-1.55 ranging from 0.8 to 4.2, whereas average incidence of TIF was 2.97+-1.47, ranging from 1.44 to 5 per cell. Statistically significant positive correlation between incidence of SCE and TIF was observed (0.77 p<0.05) indicating a connection between uncapped telomeres in these cells and homologous recombination, a link which should be further investigated.
C3  - ICEM 2009 : 10th 10th International Conference on Enviromental Mutagens : programme and the book of abstracts; Aug 20-25, 2009; Firenze, Italy
T1  - Telomere dysfunction induced foci correlates positively with incidence of sister chromatid exchanges in cells of Fanconi anemia patients
SP  - 135
SP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11711
ER  - 

@conference{
author = "Joksić, I. and Vujić, Dragana and Guć-Šćekić, Marija and Leskovac, Andreja and Petrović, Sandra and Miković, Z.",
year = "2009",
abstract = "Telomeres consist of repeated DNA sequences and associated proteins that lie at the termini of linear chromosomes and are critical to the protection and stability of internal chromosome sequences, a function known as telomere “capping”. In capping chromosomes ends, telomeres restrict chromosome end resection by exonucleases and prevent the improper activation of checkpoint response factors and DNA damage response pathways such as homologous recombination (HR) and non-homologous end joining (NHEJ). Telomeres shorten as cell divide, but also due to action of restriction exonucleases, oxidative damage and inappropriate recombination. When telomere shorten to a critical length, they become uncapped, which can lead to permanent cell cycle arrest. The development of the “TIF” (telomere dysfunction induced foci) assay that is based on colocalization of the DNA repair factors 53BP1 and γH2AX with uncapped telomeres enables measurement of telomere uncapping in different tissues. Our aim was to investigate correlation between homologous DNA recombination processes and function of telomeres. In nine primary fibroblast cell lines, obtained from patients presenting with bone marrow failure syndrome and Fanconi anemia clinical and cellular (diepoxybutane- DEB positive) phenotype, baseline incidence of SCE, T-SCE, γH2AX and TIF were examined. The average incidence of SCE was 4.42+-0.96, ranging from 3.38 to 6.5 per cell, whereas incidence of T-SCE was 1.91+-0.81 ranging from 1 to 3.33. Baseline level of γH2AX foci was 2.27+-1.55 ranging from 0.8 to 4.2, whereas average incidence of TIF was 2.97+-1.47, ranging from 1.44 to 5 per cell. Statistically significant positive correlation between incidence of SCE and TIF was observed (0.77 p<0.05) indicating a connection between uncapped telomeres in these cells and homologous recombination, a link which should be further investigated.",
journal = "ICEM 2009 : 10th 10th International Conference on Enviromental Mutagens : programme and the book of abstracts; Aug 20-25, 2009; Firenze, Italy",
title = "Telomere dysfunction induced foci correlates positively with incidence of sister chromatid exchanges in cells of Fanconi anemia patients",
pages = "135-135",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11711"
}

Joksić, I., Vujić, D., Guć-Šćekić, M., Leskovac, A., Petrović, S.,& Miković, Z.. (2009). Telomere dysfunction induced foci correlates positively with incidence of sister chromatid exchanges in cells of Fanconi anemia patients. in ICEM 2009 : 10th 10th International Conference on Enviromental Mutagens : programme and the book of abstracts; Aug 20-25, 2009; Firenze, Italy, 135.
https://hdl.handle.net/21.15107/rcub_vinar_11711

Joksić I, Vujić D, Guć-Šćekić M, Leskovac A, Petrović S, Miković Z. Telomere dysfunction induced foci correlates positively with incidence of sister chromatid exchanges in cells of Fanconi anemia patients. in ICEM 2009 : 10th 10th International Conference on Enviromental Mutagens : programme and the book of abstracts; Aug 20-25, 2009; Firenze, Italy. 2009;:135.
https://hdl.handle.net/21.15107/rcub_vinar_11711 .

Joksić, I., Vujić, Dragana, Guć-Šćekić, Marija, Leskovac, Andreja, Petrović, Sandra, Miković, Z., "Telomere dysfunction induced foci correlates positively with incidence of sister chromatid exchanges in cells of Fanconi anemia patients" in ICEM 2009 : 10th 10th International Conference on Enviromental Mutagens : programme and the book of abstracts; Aug 20-25, 2009; Firenze, Italy (2009):135,
https://hdl.handle.net/21.15107/rcub_vinar_11711 .

TY  - JOUR
AU  - Petrović, Sandra
AU  - Vujić, Dragana
AU  - Guć-Šćekić, Marija
AU  - Leskovac, Andreja
AU  - Jevtic, Dragana
AU  - Joksić, Gordana
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3751
AB  - Fanconi anemia (FA) is a genetic disease characterized by progressive pancytopenia and cancer susceptibility. The clinical and cellular phenotypes of Fanconi anemia are associated with a set of redox abnormalities, indicating that FA is an oxidative stress-related disorder. Fanconi anemia cells are highly sensitive to DNA clastogen agents, but their response to ionizing radiation is still unclear. The aim of this study was to evaluate the in vitro radiosensitivity of Fanconi anemia homozygotes and heterozygotes, and to assess the contribution of catalase and superoxide dismutase (SOD) to the overall radiobiological response of the cells. The incidence of radiation-induced lymphocyte micronuclei was used as the indicator of radiation sensitivity in vitro, whereas the activity of antioxidant enzymes was determined in erythrocytes. Patients with FA exhibited a two-fold decrease in catalase activity, accompanied by lowered activity of SOD, and increased incidence of baseline micronuclei. In the entire group of patients (with one exception), a reduced yield of radiation-induced micronuclei in lymphocytes was observed, and this was categorized as a radioresistant response. A mild radioresistant in vitro response was also observed in carrier-mothers, accompanied by reduced activity of catalase. The radiosensitivity of carrier-fathers was normal. The results of this study suggest that reduced activity of catalase is an important contributor to the radiobiological response of cells.
T2  - Archives of Biological Sciences
T1  - Influence of Catalase on the Radiosensitivity of Fanconi Anemia Lymphocytes in Vitro
VL  - 61
IS  - 2
SP  - 195
EP  - 204
DO  - 10.2298/ABS0901195P
ER  - 

@article{
author = "Petrović, Sandra and Vujić, Dragana and Guć-Šćekić, Marija and Leskovac, Andreja and Jevtic, Dragana and Joksić, Gordana",
year = "2009",
abstract = "Fanconi anemia (FA) is a genetic disease characterized by progressive pancytopenia and cancer susceptibility. The clinical and cellular phenotypes of Fanconi anemia are associated with a set of redox abnormalities, indicating that FA is an oxidative stress-related disorder. Fanconi anemia cells are highly sensitive to DNA clastogen agents, but their response to ionizing radiation is still unclear. The aim of this study was to evaluate the in vitro radiosensitivity of Fanconi anemia homozygotes and heterozygotes, and to assess the contribution of catalase and superoxide dismutase (SOD) to the overall radiobiological response of the cells. The incidence of radiation-induced lymphocyte micronuclei was used as the indicator of radiation sensitivity in vitro, whereas the activity of antioxidant enzymes was determined in erythrocytes. Patients with FA exhibited a two-fold decrease in catalase activity, accompanied by lowered activity of SOD, and increased incidence of baseline micronuclei. In the entire group of patients (with one exception), a reduced yield of radiation-induced micronuclei in lymphocytes was observed, and this was categorized as a radioresistant response. A mild radioresistant in vitro response was also observed in carrier-mothers, accompanied by reduced activity of catalase. The radiosensitivity of carrier-fathers was normal. The results of this study suggest that reduced activity of catalase is an important contributor to the radiobiological response of cells.",
journal = "Archives of Biological Sciences",
title = "Influence of Catalase on the Radiosensitivity of Fanconi Anemia Lymphocytes in Vitro",
volume = "61",
number = "2",
pages = "195-204",
doi = "10.2298/ABS0901195P"
}

Petrović, S., Vujić, D., Guć-Šćekić, M., Leskovac, A., Jevtic, D.,& Joksić, G.. (2009). Influence of Catalase on the Radiosensitivity of Fanconi Anemia Lymphocytes in Vitro. in Archives of Biological Sciences, 61(2), 195-204.
https://doi.org/10.2298/ABS0901195P

Petrović S, Vujić D, Guć-Šćekić M, Leskovac A, Jevtic D, Joksić G. Influence of Catalase on the Radiosensitivity of Fanconi Anemia Lymphocytes in Vitro. in Archives of Biological Sciences. 2009;61(2):195-204.
doi:10.2298/ABS0901195P .

Petrović, Sandra, Vujić, Dragana, Guć-Šćekić, Marija, Leskovac, Andreja, Jevtic, Dragana, Joksić, Gordana, "Influence of Catalase on the Radiosensitivity of Fanconi Anemia Lymphocytes in Vitro" in Archives of Biological Sciences, 61, no. 2 (2009):195-204,
https://doi.org/10.2298/ABS0901195P . .