TY  - JOUR
AU  - Pantelić, Marija
AU  - Jovanović, Ljubomir J.
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Ćulafić, Tijana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jpn.12655
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7804
AB  - Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods.
T2  - Journal of Animal Physiology and Animal Nutrition
T1  - The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows
VL  - 102
IS  - 1
SP  - 41
EP  - 55
DO  - 10.1111/jpn.12655
ER  - 

@article{
author = "Pantelić, Marija and Jovanović, Ljubomir J. and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Ćulafić, Tijana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2018",
abstract = "Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods.",
journal = "Journal of Animal Physiology and Animal Nutrition",
title = "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows",
volume = "102",
number = "1",
pages = "41-55",
doi = "10.1111/jpn.12655"
}

Pantelić, M., Jovanović, L. J., Prodanović, R., Vujanac, I., Đurić, M., Ćulafić, T., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D.. (2018). The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition, 102(1), 41-55.
https://doi.org/10.1111/jpn.12655

Pantelić M, Jovanović LJ, Prodanović R, Vujanac I, Đurić M, Ćulafić T, Vranješ-Đurić S, Korićanac G, Kirovski D. The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition. 2018;102(1):41-55.
doi:10.1111/jpn.12655 .

Pantelić, Marija, Jovanović, Ljubomir J., Prodanović, Radiša, Vujanac, Ivan, Đurić, Miloje, Ćulafić, Tijana, Vranješ-Đurić, Sanja, Korićanac, Goran, Kirovski, Danijela, "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows" in Journal of Animal Physiology and Animal Nutrition, 102, no. 1 (2018):41-55,
https://doi.org/10.1111/jpn.12655 . .

TY  - JOUR
AU  - Pantelić, Marija
AU  - Jovanović, Ljubomir J.
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Ćulafić, Tijana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jpn.12655
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7835
AB  - Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods. © 2017 Blackwell Verlag GmbH
T2  - Journal of Animal Physiology and Animal Nutrition
T1  - The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows
VL  - 102
IS  - 1
SP  - 41
EP  - 55
DO  - 10.1111/jpn.12655
ER  - 

@article{
author = "Pantelić, Marija and Jovanović, Ljubomir J. and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Ćulafić, Tijana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2018",
abstract = "Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods. © 2017 Blackwell Verlag GmbH",
journal = "Journal of Animal Physiology and Animal Nutrition",
title = "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows",
volume = "102",
number = "1",
pages = "41-55",
doi = "10.1111/jpn.12655"
}

Pantelić, M., Jovanović, L. J., Prodanović, R., Vujanac, I., Đurić, M., Ćulafić, T., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D.. (2018). The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition, 102(1), 41-55.
https://doi.org/10.1111/jpn.12655

Pantelić M, Jovanović LJ, Prodanović R, Vujanac I, Đurić M, Ćulafić T, Vranješ-Đurić S, Korićanac G, Kirovski D. The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition. 2018;102(1):41-55.
doi:10.1111/jpn.12655 .

Pantelić, Marija, Jovanović, Ljubomir J., Prodanović, Radiša, Vujanac, Ivan, Đurić, Miloje, Ćulafić, Tijana, Vranješ-Đurić, Sanja, Korićanac, Goran, Kirovski, Danijela, "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows" in Journal of Animal Physiology and Animal Nutrition, 102, no. 1 (2018):41-55,
https://doi.org/10.1111/jpn.12655 . .

TY  - JOUR
AU  - Nedić, Sreten
AU  - Pantelić, Marija
AU  - Vranješ-Đurić, Sanja
AU  - Nedić, Drago
AU  - Jovanović, Ljubomir J.
AU  - Cebulj-Kadunc, Nina
AU  - Kobal, Silvestra
AU  - Snoj, Tomaž
AU  - Kirovski, Danijela
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1894
AB  - Cortisol levels were measured in hair, blood and milk in two different cattle breeds, kept under different breeding conditions and with different genetic merit for milk production. Cows and heifers of Holstein and Busha breeds were selected for the study. Cortisol concentration was determined by immunoassays. Cortisol accumulation was determined in proximal (close to the skin) and distal (far from the skin) segments of the hair shaft. The influence of hair colour and washing prior to extraction and analysis was also examined in order to establish additional factors that may have an impact on hair cortisol concentrations. Concentrations of cortisol determined in the proximal and distal segments of the shaft were significantly higher in Holstein than Busha cows and heifers (P LT 0.05 and P LT 0.01, respectively). In Holstein cows, no significant difference was found between concentrations in black and white hair. In hair washed with isopropanol, cortisol concentration was significantly lower compared to unwashed hair (P LT 0.01). Thus, cortisol concentration in hair varies with the technique of hair processing (washing), but not with colour in Holstein cows. Blood serum cortisol concentrations in Holstein cows and heifers were significantly higher than in Busha cows and heifers, (P LT 0.01 and P LT 0.05, respectively). Milk cortisol in Holstein cows was significantly higher than in Busha cows (P LT 0.05). The higher cortisol concentrations in Holstein cows are assumed to be the result of intensive breeding and physiological adaptation to high milk production.
T2  - Slovenian Veterinary Research
T1  - Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle
VL  - 54
IS  - 4
SP  - 163
EP  - 172
DO  - 10.26873/SVR-398-2017
ER  - 

@article{
author = "Nedić, Sreten and Pantelić, Marija and Vranješ-Đurić, Sanja and Nedić, Drago and Jovanović, Ljubomir J. and Cebulj-Kadunc, Nina and Kobal, Silvestra and Snoj, Tomaž and Kirovski, Danijela",
year = "2017",
abstract = "Cortisol levels were measured in hair, blood and milk in two different cattle breeds, kept under different breeding conditions and with different genetic merit for milk production. Cows and heifers of Holstein and Busha breeds were selected for the study. Cortisol concentration was determined by immunoassays. Cortisol accumulation was determined in proximal (close to the skin) and distal (far from the skin) segments of the hair shaft. The influence of hair colour and washing prior to extraction and analysis was also examined in order to establish additional factors that may have an impact on hair cortisol concentrations. Concentrations of cortisol determined in the proximal and distal segments of the shaft were significantly higher in Holstein than Busha cows and heifers (P LT 0.05 and P LT 0.01, respectively). In Holstein cows, no significant difference was found between concentrations in black and white hair. In hair washed with isopropanol, cortisol concentration was significantly lower compared to unwashed hair (P LT 0.01). Thus, cortisol concentration in hair varies with the technique of hair processing (washing), but not with colour in Holstein cows. Blood serum cortisol concentrations in Holstein cows and heifers were significantly higher than in Busha cows and heifers, (P LT 0.01 and P LT 0.05, respectively). Milk cortisol in Holstein cows was significantly higher than in Busha cows (P LT 0.05). The higher cortisol concentrations in Holstein cows are assumed to be the result of intensive breeding and physiological adaptation to high milk production.",
journal = "Slovenian Veterinary Research",
title = "Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle",
volume = "54",
number = "4",
pages = "163-172",
doi = "10.26873/SVR-398-2017"
}

Nedić, S., Pantelić, M., Vranješ-Đurić, S., Nedić, D., Jovanović, L. J., Cebulj-Kadunc, N., Kobal, S., Snoj, T.,& Kirovski, D.. (2017). Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle. in Slovenian Veterinary Research, 54(4), 163-172.
https://doi.org/10.26873/SVR-398-2017

Nedić S, Pantelić M, Vranješ-Đurić S, Nedić D, Jovanović LJ, Cebulj-Kadunc N, Kobal S, Snoj T, Kirovski D. Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle. in Slovenian Veterinary Research. 2017;54(4):163-172.
doi:10.26873/SVR-398-2017 .

Nedić, Sreten, Pantelić, Marija, Vranješ-Đurić, Sanja, Nedić, Drago, Jovanović, Ljubomir J., Cebulj-Kadunc, Nina, Kobal, Silvestra, Snoj, Tomaž, Kirovski, Danijela, "Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle" in Slovenian Veterinary Research, 54, no. 4 (2017):163-172,
https://doi.org/10.26873/SVR-398-2017 . .

TY  - JOUR
AU  - Jovanović, Ljubomir J.
AU  - Pantelić, Marija
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Tepavčević, Snežana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1808
AB  - The objective of this study was to investigate the effects of peroral administration of chromium-enriched yeast on glucose tolerance in Holstein calves, assessed by insulin signaling pathway molecule determination and intravenous glucose tolerance test (IVGTT). Twenty-four Holstein calves, aged 1 month, were chosen for the study and divided into two groups: the PoCr group (n = 12) that perorally received 0.04 mg of Cr/kg of body mass daily, for 70 days, and the NCr group (n = 12) that received no chromium supplementation. Skeletal tissue samples from each calf were obtained on day 0 and day 70 of the experiment. Chromium supplementation increased protein content of the insulin beta-subunit receptor, phosphorylation of insulin receptor substrate 1 at Tyrosine 632, phosphorylation of Akt at Serine 473, glucose transporter-4, and AMP-activated protein kinase in skeletal muscle tissue, while phosphorylation of insulin receptor substrate 1 at Serine 307 was not affected by chromium treatment. Results obtained during IVGTT, which was conducted on days 0, 30, 50, and 70, suggested an increased insulin sensitivity and, consequently, a better utilization of glucose in the PoCr group. Lower basal concentrations of glucose and insulin in the PoCr group on days 30 and 70 were also obtained. Our results indicate that chromium supplementation improves glucose utilization in calves by enhancing insulin intracellular signaling in the skeletal muscle tissue.
T2  - Biological Trace Element Research
T1  - Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves
VL  - 180
IS  - 2
SP  - 223
EP  - 232
DO  - 10.1007/s12011-017-1007-1
ER  - 

@article{
author = "Jovanović, Ljubomir J. and Pantelić, Marija and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Tepavčević, Snežana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2017",
abstract = "The objective of this study was to investigate the effects of peroral administration of chromium-enriched yeast on glucose tolerance in Holstein calves, assessed by insulin signaling pathway molecule determination and intravenous glucose tolerance test (IVGTT). Twenty-four Holstein calves, aged 1 month, were chosen for the study and divided into two groups: the PoCr group (n = 12) that perorally received 0.04 mg of Cr/kg of body mass daily, for 70 days, and the NCr group (n = 12) that received no chromium supplementation. Skeletal tissue samples from each calf were obtained on day 0 and day 70 of the experiment. Chromium supplementation increased protein content of the insulin beta-subunit receptor, phosphorylation of insulin receptor substrate 1 at Tyrosine 632, phosphorylation of Akt at Serine 473, glucose transporter-4, and AMP-activated protein kinase in skeletal muscle tissue, while phosphorylation of insulin receptor substrate 1 at Serine 307 was not affected by chromium treatment. Results obtained during IVGTT, which was conducted on days 0, 30, 50, and 70, suggested an increased insulin sensitivity and, consequently, a better utilization of glucose in the PoCr group. Lower basal concentrations of glucose and insulin in the PoCr group on days 30 and 70 were also obtained. Our results indicate that chromium supplementation improves glucose utilization in calves by enhancing insulin intracellular signaling in the skeletal muscle tissue.",
journal = "Biological Trace Element Research",
title = "Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves",
volume = "180",
number = "2",
pages = "223-232",
doi = "10.1007/s12011-017-1007-1"
}

Jovanović, L. J., Pantelić, M., Prodanović, R., Vujanac, I., Đurić, M., Tepavčević, S., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D.. (2017). Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves. in Biological Trace Element Research, 180(2), 223-232.
https://doi.org/10.1007/s12011-017-1007-1

Jovanović LJ, Pantelić M, Prodanović R, Vujanac I, Đurić M, Tepavčević S, Vranješ-Đurić S, Korićanac G, Kirovski D. Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves. in Biological Trace Element Research. 2017;180(2):223-232.
doi:10.1007/s12011-017-1007-1 .

Jovanović, Ljubomir J., Pantelić, Marija, Prodanović, Radiša, Vujanac, Ivan, Đurić, Miloje, Tepavčević, Snežana, Vranješ-Đurić, Sanja, Korićanac, Goran, Kirovski, Danijela, "Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves" in Biological Trace Element Research, 180, no. 2 (2017):223-232,
https://doi.org/10.1007/s12011-017-1007-1 . .