Markovic, Ljiljana

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  • Markovic, Ljiljana (3)
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Author's Bibliography

A Bioinformatics Approach to Identify Natural Autoantibodies from Healthy Blood Donors Sera Reactive with the HCV NS5A-Derived Peptide by Immunoassay

Vasiljevic, Nada; Veljković, Nevena V.; Kosec, Tatjana; Ma, Xue-Zhong; Glišić, Sanja; Prljić, Jelena; Vujicic, Ana Djordjevic; Markovic, Ljiljana; Branch, Donald R.

(2011)

TY  - JOUR
AU  - Vasiljevic, Nada
AU  - Veljković, Nevena V.
AU  - Kosec, Tatjana
AU  - Ma, Xue-Zhong
AU  - Glišić, Sanja
AU  - Prljić, Jelena
AU  - Vujicic, Ana Djordjevic
AU  - Markovic, Ljiljana
AU  - Branch, Donald R.
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4259
AB  - Natural autoantibodies (NAbs) are continually produced throughout life and have an ability to recognize self and altered self, as well as foreign antigens, by recognizing cellular pattern recognition receptors. Sometimes NAb specificity demonstrates overlap between human and pathologic proteomes. This information can be useful in selecting target sequences for screening purposes. In this study we undertook a multi-step bioinformatics search to predict a virus-derived peptide that can be recognized by NAbs in sera of uninfected individuals. We selected protein hepatitis C virus (HCV) NS5A as a target sequence, motivated by the fact that the HCV proteome is characterized by extensive sequence similarities to the human proteome, and because screening for anti-HCV antibodies, including anti-NS5A, is important clinically, particularly in screening of potential blood donors. The virus-specific peptide P1, and the homologous human peptide derived from enzyme-inducible nitric oxide synthase (iNOS), P2, exhibiting not only simple homology, but also complementarities of physicochemical patterns, were synthesized and 80 HCV-negative and 50 HCV-positive blood donor sera were tested by ELISA. These peptides reacted similarly (p LT 0.001) with HCV-negative sera, and in several cases the measured reactivity was significantly above the cut-off value of commercial anti-HCV screening assays. In HCV-positive sera, the titers of antibodies reactive with analyzed HCV NS5A peptide were not significantly increased (p LT 0.001) compared to host peptide, the implications of which are unclear, but may be consistent with these antibodies being naturally produced. Finally, we extended our bioinformatics analyses to the dataset of human self-binding sequences, and propose a general approach for the selection of specific diagnostic and screening antigens for use in immunoassays.
T2  - Viral Immunology
T1  - A Bioinformatics Approach to Identify Natural Autoantibodies from Healthy Blood Donors Sera Reactive with the HCV NS5A-Derived Peptide by Immunoassay
VL  - 24
IS  - 2
SP  - 69
EP  - 76
DO  - 10.1089/vim.2010.0107
ER  - 
@article{
author = "Vasiljevic, Nada and Veljković, Nevena V. and Kosec, Tatjana and Ma, Xue-Zhong and Glišić, Sanja and Prljić, Jelena and Vujicic, Ana Djordjevic and Markovic, Ljiljana and Branch, Donald R.",
year = "2011",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4259",
abstract = "Natural autoantibodies (NAbs) are continually produced throughout life and have an ability to recognize self and altered self, as well as foreign antigens, by recognizing cellular pattern recognition receptors. Sometimes NAb specificity demonstrates overlap between human and pathologic proteomes. This information can be useful in selecting target sequences for screening purposes. In this study we undertook a multi-step bioinformatics search to predict a virus-derived peptide that can be recognized by NAbs in sera of uninfected individuals. We selected protein hepatitis C virus (HCV) NS5A as a target sequence, motivated by the fact that the HCV proteome is characterized by extensive sequence similarities to the human proteome, and because screening for anti-HCV antibodies, including anti-NS5A, is important clinically, particularly in screening of potential blood donors. The virus-specific peptide P1, and the homologous human peptide derived from enzyme-inducible nitric oxide synthase (iNOS), P2, exhibiting not only simple homology, but also complementarities of physicochemical patterns, were synthesized and 80 HCV-negative and 50 HCV-positive blood donor sera were tested by ELISA. These peptides reacted similarly (p LT 0.001) with HCV-negative sera, and in several cases the measured reactivity was significantly above the cut-off value of commercial anti-HCV screening assays. In HCV-positive sera, the titers of antibodies reactive with analyzed HCV NS5A peptide were not significantly increased (p LT 0.001) compared to host peptide, the implications of which are unclear, but may be consistent with these antibodies being naturally produced. Finally, we extended our bioinformatics analyses to the dataset of human self-binding sequences, and propose a general approach for the selection of specific diagnostic and screening antigens for use in immunoassays.",
journal = "Viral Immunology",
title = "A Bioinformatics Approach to Identify Natural Autoantibodies from Healthy Blood Donors Sera Reactive with the HCV NS5A-Derived Peptide by Immunoassay",
volume = "24",
number = "2",
pages = "69-76",
doi = "10.1089/vim.2010.0107"
}
Vasiljevic, N., Veljković, N. V., Kosec, T., Ma, X., Glišić, S., Prljić, J., Vujicic, A. D., Markovic, L.,& Branch, D. R. (2011). A Bioinformatics Approach to Identify Natural Autoantibodies from Healthy Blood Donors Sera Reactive with the HCV NS5A-Derived Peptide by Immunoassay.
Viral Immunology, 24(2), 69-76.
https://doi.org/10.1089/vim.2010.0107
Vasiljevic N, Veljković NV, Kosec T, Ma X, Glišić S, Prljić J, Vujicic AD, Markovic L, Branch DR. A Bioinformatics Approach to Identify Natural Autoantibodies from Healthy Blood Donors Sera Reactive with the HCV NS5A-Derived Peptide by Immunoassay. Viral Immunology. 2011;24(2):69-76
Vasiljevic Nada, Veljković Nevena V., Kosec Tatjana, Ma Xue-Zhong, Glišić Sanja, Prljić Jelena, Vujicic Ana Djordjevic, Markovic Ljiljana, Branch Donald R., "A Bioinformatics Approach to Identify Natural Autoantibodies from Healthy Blood Donors Sera Reactive with the HCV NS5A-Derived Peptide by Immunoassay" Viral Immunology, 24, no. 2 (2011):69-76,
https://doi.org/10.1089/vim.2010.0107 .
1

Heavy Metal and Bacterial Pollution of the Sava River in Serbia

Vuković, Živorad; Markovic, Ljiljana; Radenković, Mirjana; Vukovic, Dubravka; Stanković, Srboljub J.

(2011)

TY  - JOUR
AU  - Vuković, Živorad
AU  - Markovic, Ljiljana
AU  - Radenković, Mirjana
AU  - Vukovic, Dubravka
AU  - Stanković, Srboljub J.
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4256
AB  - The aim of this study was to establish microbial and heavy metal pollution of the Sava River at three locations close to industry and urban areas (Sabac, Obrenovac, Beograd) in Serbia. Heavy metal analysis included Cu, Zn, Pb, and Cd in the river water and sediment samples. Using the microbiological analysis we tried to establish the effectiveness of total coliforms, faecal conforms and Escherichia coli in detecting pollution of surface waters. We found that E. coli levels steadily increased downstream from Sabac (location 1; 2100 MPN per 100 mL) to Belgrade (location 3; 10000 MPN per 100 mL). To prevent bacterial contamination, it is necessary to reduce the discharge of wastewater with faecal matters near highly populated towns. Heavy metal levels in sediments correlated with those in the river water. Fluctuations attributed mainly to anthropogenic sources were not high. These results point to acceptable anthropogenic contribution to heavy metal content in the Sava River and to low environmental risk.
T2  - Arhiv za higijenu rada i toksikologiju
T1  - Heavy Metal and Bacterial Pollution of the Sava River in Serbia
VL  - 62
IS  - 1
SP  - 11
EP  - 16
DO  - 10.2478/10004-1254-62-2011-2051
ER  - 
@article{
author = "Vuković, Živorad and Markovic, Ljiljana and Radenković, Mirjana and Vukovic, Dubravka and Stanković, Srboljub J.",
year = "2011",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4256",
abstract = "The aim of this study was to establish microbial and heavy metal pollution of the Sava River at three locations close to industry and urban areas (Sabac, Obrenovac, Beograd) in Serbia. Heavy metal analysis included Cu, Zn, Pb, and Cd in the river water and sediment samples. Using the microbiological analysis we tried to establish the effectiveness of total coliforms, faecal conforms and Escherichia coli in detecting pollution of surface waters. We found that E. coli levels steadily increased downstream from Sabac (location 1; 2100 MPN per 100 mL) to Belgrade (location 3; 10000 MPN per 100 mL). To prevent bacterial contamination, it is necessary to reduce the discharge of wastewater with faecal matters near highly populated towns. Heavy metal levels in sediments correlated with those in the river water. Fluctuations attributed mainly to anthropogenic sources were not high. These results point to acceptable anthropogenic contribution to heavy metal content in the Sava River and to low environmental risk.",
journal = "Arhiv za higijenu rada i toksikologiju",
title = "Heavy Metal and Bacterial Pollution of the Sava River in Serbia",
volume = "62",
number = "1",
pages = "11-16",
doi = "10.2478/10004-1254-62-2011-2051"
}
Vuković, Ž., Markovic, L., Radenković, M., Vukovic, D.,& Stanković, S. J. (2011). Heavy Metal and Bacterial Pollution of the Sava River in Serbia.
Arhiv za higijenu rada i toksikologiju, 62(1), 11-16.
https://doi.org/10.2478/10004-1254-62-2011-2051
Vuković Ž, Markovic L, Radenković M, Vukovic D, Stanković SJ. Heavy Metal and Bacterial Pollution of the Sava River in Serbia. Arhiv za higijenu rada i toksikologiju. 2011;62(1):11-16
Vuković Živorad, Markovic Ljiljana, Radenković Mirjana, Vukovic Dubravka, Stanković Srboljub J., "Heavy Metal and Bacterial Pollution of the Sava River in Serbia" Arhiv za higijenu rada i toksikologiju, 62, no. 1 (2011):11-16,
https://doi.org/10.2478/10004-1254-62-2011-2051 .
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Regulation of the inducible nitric oxide synthase and sodium pump in type 1 diabetes

Žakula, Zorica; Korićanac, Goran; Putnikovic, Biljana; Markovic, Ljiljana; Isenović, Esma R.

(2007)

TY  - JOUR
AU  - Žakula, Zorica
AU  - Korićanac, Goran
AU  - Putnikovic, Biljana
AU  - Markovic, Ljiljana
AU  - Isenović, Esma R.
PY  - 2007
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3205
AB  - Insulin-like growth factor-1 (IGF-1) is a hormone and growth factor closely related to insulin. The autocrine/paracrine actions of IGF-1 involve activation of inducible nitric oxide synthase (iNOS) and the Na(+), K(+)-ATPase sodium pump in cardiovascular tissues. Data from literature indicate that iNOS is expressed in vascular smooth muscle cells (VSMC) and that IGF- 1 -induced release of NO is both rapid and delayed. We hypothesize that impaired IGF-1 -induced sodium pump activity/ expression in rats with type 1 diabetes is related to activation of phosphatidytinositol 3 kinase (PI3K)/cytosolic phospholipase 2 (cPLA(2))/protein kinase B (Akt) signaling, and that IGF-1 prevents acute and chronic dysfunction of iNOS and sodium pump activity in a chemically induced model of type 1 diabetes, the streptozotocin -treated rat heart (STZ). Understanding how iNOS and sodium pump activity are regulated by IGF-1 activation of the PI3K/cPLA(2)/Akt cascade should provide novel and fundamental knowledge regarding the regulatory actions of IGF-1 in promoting vasodilation. Since insulin resistance is currently a major focus of research, the use of IGF-1 to improve insulin resistance and glucose metabolism has opened a new arena for treatment of comorbid conditions. Future investigations should now focus on mechanisms of action of IGF-1 and its clinical applicability. (c) 2007 Elsevier Ltd. All rights reserved.
T2  - Medical Hypotheses
T1  - Regulation of the inducible nitric oxide synthase and sodium pump in type 1 diabetes
VL  - 69
IS  - 2
SP  - 302
EP  - 306
DO  - 10.1016/j.mehy.2006.11.045
ER  - 
@article{
author = "Žakula, Zorica and Korićanac, Goran and Putnikovic, Biljana and Markovic, Ljiljana and Isenović, Esma R.",
year = "2007",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3205",
abstract = "Insulin-like growth factor-1 (IGF-1) is a hormone and growth factor closely related to insulin. The autocrine/paracrine actions of IGF-1 involve activation of inducible nitric oxide synthase (iNOS) and the Na(+), K(+)-ATPase sodium pump in cardiovascular tissues. Data from literature indicate that iNOS is expressed in vascular smooth muscle cells (VSMC) and that IGF- 1 -induced release of NO is both rapid and delayed. We hypothesize that impaired IGF-1 -induced sodium pump activity/ expression in rats with type 1 diabetes is related to activation of phosphatidytinositol 3 kinase (PI3K)/cytosolic phospholipase 2 (cPLA(2))/protein kinase B (Akt) signaling, and that IGF-1 prevents acute and chronic dysfunction of iNOS and sodium pump activity in a chemically induced model of type 1 diabetes, the streptozotocin -treated rat heart (STZ). Understanding how iNOS and sodium pump activity are regulated by IGF-1 activation of the PI3K/cPLA(2)/Akt cascade should provide novel and fundamental knowledge regarding the regulatory actions of IGF-1 in promoting vasodilation. Since insulin resistance is currently a major focus of research, the use of IGF-1 to improve insulin resistance and glucose metabolism has opened a new arena for treatment of comorbid conditions. Future investigations should now focus on mechanisms of action of IGF-1 and its clinical applicability. (c) 2007 Elsevier Ltd. All rights reserved.",
journal = "Medical Hypotheses",
title = "Regulation of the inducible nitric oxide synthase and sodium pump in type 1 diabetes",
volume = "69",
number = "2",
pages = "302-306",
doi = "10.1016/j.mehy.2006.11.045"
}
Žakula, Z., Korićanac, G., Putnikovic, B., Markovic, L.,& Isenović, E. R. (2007). Regulation of the inducible nitric oxide synthase and sodium pump in type 1 diabetes.
Medical Hypotheses, 69(2), 302-306.
https://doi.org/10.1016/j.mehy.2006.11.045
Žakula Z, Korićanac G, Putnikovic B, Markovic L, Isenović ER. Regulation of the inducible nitric oxide synthase and sodium pump in type 1 diabetes. Medical Hypotheses. 2007;69(2):302-306
Žakula Zorica, Korićanac Goran, Putnikovic Biljana, Markovic Ljiljana, Isenović Esma R., "Regulation of the inducible nitric oxide synthase and sodium pump in type 1 diabetes" Medical Hypotheses, 69, no. 2 (2007):302-306,
https://doi.org/10.1016/j.mehy.2006.11.045 .
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