TY  - CONF
AU  - Butulija, Svetlana
AU  - Filipović Tričković, Jelena
AU  - Valenta Šobot, Ana
AU  - Todorović, Bratislav
AU  - Petrović, Sanja
AU  - Ilić, Bojana
AU  - Zmejkoski, Danica
AU  - Matović, Branko
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13167
PB  - Belgrade : Vinča Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade
PB  - Belgrade : Serbian Society for Innovative Materials in Extreme Conditions (SIM-EXTREME)
C3  - IMEC2024 - 2nd International Conference on Innovative Materials in Extreme Conditions : Book of abstracts
T1  - Bacterial Cellulose-Cerium Oxide Hydrogel for Tailored Redox Balance in Biomedical Extremes
SP  - 73
EP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13167
ER  - 

@conference{
author = "Butulija, Svetlana and Filipović Tričković, Jelena and Valenta Šobot, Ana and Todorović, Bratislav and Petrović, Sanja and Ilić, Bojana and Zmejkoski, Danica and Matović, Branko",
year = "2024",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade, Belgrade : Serbian Society for Innovative Materials in Extreme Conditions (SIM-EXTREME)",
journal = "IMEC2024 - 2nd International Conference on Innovative Materials in Extreme Conditions : Book of abstracts",
title = "Bacterial Cellulose-Cerium Oxide Hydrogel for Tailored Redox Balance in Biomedical Extremes",
pages = "73-73",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13167"
}

Butulija, S., Filipović Tričković, J., Valenta Šobot, A., Todorović, B., Petrović, S., Ilić, B., Zmejkoski, D.,& Matović, B.. (2024). Bacterial Cellulose-Cerium Oxide Hydrogel for Tailored Redox Balance in Biomedical Extremes. in IMEC2024 - 2nd International Conference on Innovative Materials in Extreme Conditions : Book of abstracts
Belgrade : Vinča Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade., 73-73.
https://hdl.handle.net/21.15107/rcub_vinar_13167

Butulija S, Filipović Tričković J, Valenta Šobot A, Todorović B, Petrović S, Ilić B, Zmejkoski D, Matović B. Bacterial Cellulose-Cerium Oxide Hydrogel for Tailored Redox Balance in Biomedical Extremes. in IMEC2024 - 2nd International Conference on Innovative Materials in Extreme Conditions : Book of abstracts. 2024;:73-73.
https://hdl.handle.net/21.15107/rcub_vinar_13167 .

Butulija, Svetlana, Filipović Tričković, Jelena, Valenta Šobot, Ana, Todorović, Bratislav, Petrović, Sanja, Ilić, Bojana, Zmejkoski, Danica, Matović, Branko, "Bacterial Cellulose-Cerium Oxide Hydrogel for Tailored Redox Balance in Biomedical Extremes" in IMEC2024 - 2nd International Conference on Innovative Materials in Extreme Conditions : Book of abstracts (2024):73-73,
https://hdl.handle.net/21.15107/rcub_vinar_13167 .

TY  - JOUR
AU  - Algarra, Manuel
AU  - Carrillo, Celia
AU  - Nešić, Maja D.
AU  - Filipović Tričković, Jelena
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Jiménez-Jiménez, José
AU  - Rodriguez-Castellón, Enrique
AU  - Bandosz, Teresa J.
AU  - Petković, Marijana
AU  - Soto, Juan
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12247
AB  - This paper explains the basis for the excitation energy-independent fluorescence emission of biomass-derived carbon dots (CDs) and shows that these CDs have excellent anti-melanoma and anti-metastatic potential. Additionally, we demonstrate that the black carrots´-derived CDs can be exploited as cell cycle-sensing agents, because of the interaction with chromatin material. Besides their optical properties, fluorescent CDs have gained increased attention for image-guided cancer treatment due to their water solubility, environmental friendliness, affordability, ease of synthesis, and primary biocompatibility. CDs have excellent photostability, determined by their precursors and synthesis pathways. In this study, CDs with chemically homogenous surface functional groups were made using a hydrothermal technique from black carrot extract, an anthocyanin-rich substance derived from biomass. The anti-cancer and anti-metastatic properties of black carrot-derived CDs can be attributed to flavylium cations on the surface, spherical forms, and high water dispersibility. Most importantly, these CDs demonstrate a stable emission at a single wavelength, 612 nm, independent of the excitation energy, which we have explained theoretically for the first time.
T2  - Journal of Molecular Structure
T1  - Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential
VL  - 1300
SP  - 137245
DO  - 10.1016/j.molstruc.2023.137245
ER  - 

@article{
author = "Algarra, Manuel and Carrillo, Celia and Nešić, Maja D. and Filipović Tričković, Jelena and Žakula, Jelena and Korićanac, Lela and Jiménez-Jiménez, José and Rodriguez-Castellón, Enrique and Bandosz, Teresa J. and Petković, Marijana and Soto, Juan",
year = "2024",
abstract = "This paper explains the basis for the excitation energy-independent fluorescence emission of biomass-derived carbon dots (CDs) and shows that these CDs have excellent anti-melanoma and anti-metastatic potential. Additionally, we demonstrate that the black carrots´-derived CDs can be exploited as cell cycle-sensing agents, because of the interaction with chromatin material. Besides their optical properties, fluorescent CDs have gained increased attention for image-guided cancer treatment due to their water solubility, environmental friendliness, affordability, ease of synthesis, and primary biocompatibility. CDs have excellent photostability, determined by their precursors and synthesis pathways. In this study, CDs with chemically homogenous surface functional groups were made using a hydrothermal technique from black carrot extract, an anthocyanin-rich substance derived from biomass. The anti-cancer and anti-metastatic properties of black carrot-derived CDs can be attributed to flavylium cations on the surface, spherical forms, and high water dispersibility. Most importantly, these CDs demonstrate a stable emission at a single wavelength, 612 nm, independent of the excitation energy, which we have explained theoretically for the first time.",
journal = "Journal of Molecular Structure",
title = "Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential",
volume = "1300",
pages = "137245",
doi = "10.1016/j.molstruc.2023.137245"
}

Algarra, M., Carrillo, C., Nešić, M. D., Filipović Tričković, J., Žakula, J., Korićanac, L., Jiménez-Jiménez, J., Rodriguez-Castellón, E., Bandosz, T. J., Petković, M.,& Soto, J.. (2024). Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential. in Journal of Molecular Structure, 1300, 137245.
https://doi.org/10.1016/j.molstruc.2023.137245

Algarra M, Carrillo C, Nešić MD, Filipović Tričković J, Žakula J, Korićanac L, Jiménez-Jiménez J, Rodriguez-Castellón E, Bandosz TJ, Petković M, Soto J. Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential. in Journal of Molecular Structure. 2024;1300:137245.
doi:10.1016/j.molstruc.2023.137245 .

Algarra, Manuel, Carrillo, Celia, Nešić, Maja D., Filipović Tričković, Jelena, Žakula, Jelena, Korićanac, Lela, Jiménez-Jiménez, José, Rodriguez-Castellón, Enrique, Bandosz, Teresa J., Petković, Marijana, Soto, Juan, "Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential" in Journal of Molecular Structure, 1300 (2024):137245,
https://doi.org/10.1016/j.molstruc.2023.137245 . .

TY  - JOUR
AU  - Filipović Tričković, Jelena G.
AU  - Momčilović, Miloš
AU  - Joksić, Gordana
AU  - Živković, Sanja
AU  - Ilić, Bojana
AU  - Ognjanović, Miloš
AU  - Novaković, Mirjana
AU  - Valenta-Šobot, Ana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11376
AB  - Silver nanoparticles (AgNPs) have been recognized for widespread biological applications due to their antimicrobial and anti-inflammatory effect, especially in dentistry and for wound healing. Many features determine their beneficial or toxic potential, such as their synthesis type, size, morphology, coating, and concentration. Most synthesis types rely on the use of synthetic chemicals, which contributes to their toxicity. We present an environmentally friendly method for “green” synthesis of AgNPs from the silver target by pulsed laser ablation in liquid (PLAL) using citrate as the stabilizing agent. Since AgNPs already have many dental applications, we examined their antibacterial effect against supragingival biofilm-forming bacteria and bacterial strains known to cause resistant dental infections. Their impact on human fibroblast cells’ cytotoxicity, proliferation (measured by XTT and Ki-67 immunofluorescence), pro/antioxidant balance, and lipid peroxidation (measured by PAB and LPP) was evaluated. AgNPs1 (21 nm) and AgNPs2 (15 nm) spherical nanoparticles with good overall stability were obtained. The highest tested dose of smaller nanoparticles (AgNPs2) displays not only an effective antibacterial effect against the tested oral bacteria strains but also a pro-oxidant and cytotoxic effect on fibroblast cells. Lower doses do not affect bacterial survival but increase the cell proliferation and metabolic activity and show an antioxidative effect, suggesting that different concentrations display a substantially opposite effect. Compared to larger AgNPs1, smaller AgNPs2 possess more potent biological effects, indicating that size plays a pivotal role in their activity. Such opposite outcomes could be important for their medical application, and high concentrations could be used for the inhibition of dental biofilm formation and resistant dental infections as well as proliferative conditions, while low doses could be beneficial in the treatment of atrophic and inflammatory disorders. Finally, we showed that silver-targeted PLAL, using citrate as a stabilizing agent, produces biologically potent nanoparticles that could have many applications depending on their size and concentration.
T2  - Nanomaterials and Nanotechnology
T1  - Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects
VL  - 2023
SP  - e9854356
DO  - 10.1155/2023/9854356
ER  - 

@article{
author = "Filipović Tričković, Jelena G. and Momčilović, Miloš and Joksić, Gordana and Živković, Sanja and Ilić, Bojana and Ognjanović, Miloš and Novaković, Mirjana and Valenta-Šobot, Ana",
year = "2023",
abstract = "Silver nanoparticles (AgNPs) have been recognized for widespread biological applications due to their antimicrobial and anti-inflammatory effect, especially in dentistry and for wound healing. Many features determine their beneficial or toxic potential, such as their synthesis type, size, morphology, coating, and concentration. Most synthesis types rely on the use of synthetic chemicals, which contributes to their toxicity. We present an environmentally friendly method for “green” synthesis of AgNPs from the silver target by pulsed laser ablation in liquid (PLAL) using citrate as the stabilizing agent. Since AgNPs already have many dental applications, we examined their antibacterial effect against supragingival biofilm-forming bacteria and bacterial strains known to cause resistant dental infections. Their impact on human fibroblast cells’ cytotoxicity, proliferation (measured by XTT and Ki-67 immunofluorescence), pro/antioxidant balance, and lipid peroxidation (measured by PAB and LPP) was evaluated. AgNPs1 (21 nm) and AgNPs2 (15 nm) spherical nanoparticles with good overall stability were obtained. The highest tested dose of smaller nanoparticles (AgNPs2) displays not only an effective antibacterial effect against the tested oral bacteria strains but also a pro-oxidant and cytotoxic effect on fibroblast cells. Lower doses do not affect bacterial survival but increase the cell proliferation and metabolic activity and show an antioxidative effect, suggesting that different concentrations display a substantially opposite effect. Compared to larger AgNPs1, smaller AgNPs2 possess more potent biological effects, indicating that size plays a pivotal role in their activity. Such opposite outcomes could be important for their medical application, and high concentrations could be used for the inhibition of dental biofilm formation and resistant dental infections as well as proliferative conditions, while low doses could be beneficial in the treatment of atrophic and inflammatory disorders. Finally, we showed that silver-targeted PLAL, using citrate as a stabilizing agent, produces biologically potent nanoparticles that could have many applications depending on their size and concentration.",
journal = "Nanomaterials and Nanotechnology",
title = "Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects",
volume = "2023",
pages = "e9854356",
doi = "10.1155/2023/9854356"
}

Filipović Tričković, J. G., Momčilović, M., Joksić, G., Živković, S., Ilić, B., Ognjanović, M., Novaković, M.,& Valenta-Šobot, A.. (2023). Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects. in Nanomaterials and Nanotechnology, 2023, e9854356.
https://doi.org/10.1155/2023/9854356

Filipović Tričković JG, Momčilović M, Joksić G, Živković S, Ilić B, Ognjanović M, Novaković M, Valenta-Šobot A. Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects. in Nanomaterials and Nanotechnology. 2023;2023:e9854356.
doi:10.1155/2023/9854356 .

Filipović Tričković, Jelena G., Momčilović, Miloš, Joksić, Gordana, Živković, Sanja, Ilić, Bojana, Ognjanović, Miloš, Novaković, Mirjana, Valenta-Šobot, Ana, "Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects" in Nanomaterials and Nanotechnology, 2023 (2023):e9854356,
https://doi.org/10.1155/2023/9854356 . .

TY  - CONF
AU  - Kokunešoski, Maja
AU  - Šaponjić, Aleksandra
AU  - Baščarević, Zvezdana
AU  - Jovanović, Ivan
AU  - Filipović Tričković, Jelena G.
AU  - Valenta-Šobot, Ana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11280
AB  - Material SBA-15 with all of its particles in the form of regular spheres was synthesised by template method using
Pluronic P123 (non-ionic triblock copolymer) as a surfactant and tetraethoxysilane (TEOS, 98%) as a source of silica.
The synthesis of spherical SBA-15 was carried out in acidic conditions according to method proposed by Zhao et al.
Instead of expensive commercial cetyltrimethylammonium bromide as a co-surfactant, a spent HCl solution obtained
after the chemical treatment of clay in an aqueous solution of HCl was used. The mixture was aged at a higher
temperature. After that, the spherical SBA-15 was made by drying at room temperature after washing with distilled
water and then calcining in flowing air to decompose the triblock copolymer. The continual presence of spheres
of diameters around 2 μm in the entire SBA-15 was confirmed by scanning electron microscopy. The Energy-dispersive
X-ray spectroscopy confirmed that the spheres consisted only of SiO2 in composition. In addition, X-ray diffraction and
Fourier-transform infrared spectroscopy methods were used to characterise SBA-15 material. To assess the cytotoxic
effects of newly synthesised SBA-15 material, HEK 293 cells were treated with particles and extracts obtained from
particles incubated 24 hours at 37 °C in serum-free DMEM medium and DMEM with 10% serum. Cells were treated
with concentrations of 100, 250, and 500 µg/mL for 72 hours. All treatments reduced cell viability in a concentrationdependent manner (p<0.001). Cytotoxicity was more pronounced in particle treatment, indicating that mechanical
damage was induced in cells, whereas the extraction process influences the level of toxicity since the sera-free medium
had the mildest effect. To acquire safe particles for human use, additional synthesis optimisation is required.
PB  - Association of Metallurgical Engineers of Serbia (AMES)
C3  - MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina
T1  - Preliminary cytotoxicity testing of newly synthesised SBA-15 material
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11280
ER  - 

@conference{
author = "Kokunešoski, Maja and Šaponjić, Aleksandra and Baščarević, Zvezdana and Jovanović, Ivan and Filipović Tričković, Jelena G. and Valenta-Šobot, Ana",
year = "2023",
abstract = "Material SBA-15 with all of its particles in the form of regular spheres was synthesised by template method using
Pluronic P123 (non-ionic triblock copolymer) as a surfactant and tetraethoxysilane (TEOS, 98%) as a source of silica.
The synthesis of spherical SBA-15 was carried out in acidic conditions according to method proposed by Zhao et al.
Instead of expensive commercial cetyltrimethylammonium bromide as a co-surfactant, a spent HCl solution obtained
after the chemical treatment of clay in an aqueous solution of HCl was used. The mixture was aged at a higher
temperature. After that, the spherical SBA-15 was made by drying at room temperature after washing with distilled
water and then calcining in flowing air to decompose the triblock copolymer. The continual presence of spheres
of diameters around 2 μm in the entire SBA-15 was confirmed by scanning electron microscopy. The Energy-dispersive
X-ray spectroscopy confirmed that the spheres consisted only of SiO2 in composition. In addition, X-ray diffraction and
Fourier-transform infrared spectroscopy methods were used to characterise SBA-15 material. To assess the cytotoxic
effects of newly synthesised SBA-15 material, HEK 293 cells were treated with particles and extracts obtained from
particles incubated 24 hours at 37 °C in serum-free DMEM medium and DMEM with 10% serum. Cells were treated
with concentrations of 100, 250, and 500 µg/mL for 72 hours. All treatments reduced cell viability in a concentrationdependent manner (p<0.001). Cytotoxicity was more pronounced in particle treatment, indicating that mechanical
damage was induced in cells, whereas the extraction process influences the level of toxicity since the sera-free medium
had the mildest effect. To acquire safe particles for human use, additional synthesis optimisation is required.",
publisher = "Association of Metallurgical Engineers of Serbia (AMES)",
journal = "MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina",
title = "Preliminary cytotoxicity testing of newly synthesised SBA-15 material",
pages = "60",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11280"
}

Kokunešoski, M., Šaponjić, A., Baščarević, Z., Jovanović, I., Filipović Tričković, J. G.,& Valenta-Šobot, A.. (2023). Preliminary cytotoxicity testing of newly synthesised SBA-15 material. in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina
Association of Metallurgical Engineers of Serbia (AMES)., 60.
https://hdl.handle.net/21.15107/rcub_vinar_11280

Kokunešoski M, Šaponjić A, Baščarević Z, Jovanović I, Filipović Tričković JG, Valenta-Šobot A. Preliminary cytotoxicity testing of newly synthesised SBA-15 material. in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina. 2023;:60.
https://hdl.handle.net/21.15107/rcub_vinar_11280 .

Kokunešoski, Maja, Šaponjić, Aleksandra, Baščarević, Zvezdana, Jovanović, Ivan, Filipović Tričković, Jelena G., Valenta-Šobot, Ana, "Preliminary cytotoxicity testing of newly synthesised SBA-15 material" in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina (2023):60,
https://hdl.handle.net/21.15107/rcub_vinar_11280 .

TY  - CONF
AU  - Filipović Tričković, Jelena G.
AU  - Kokunešoski, Maja
AU  - Momić, Tatjana
AU  - Jovanović, Ivan G.
AU  - Anastasov, Marina
AU  - Stojanović, Dragana
AU  - Valenta Šobot, Ana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11409
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book
T1  - Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures
T1  - Procena toksičnosti mezoporoznih nanočestica silicijum dioksida pri različitim postupcima ekstrakcije
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11409
ER  - 

@conference{
author = "Filipović Tričković, Jelena G. and Kokunešoski, Maja and Momić, Tatjana and Jovanović, Ivan G. and Anastasov, Marina and Stojanović, Dragana and Valenta Šobot, Ana",
year = "2023",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book",
title = "Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures, Procena toksičnosti mezoporoznih nanočestica silicijum dioksida pri različitim postupcima ekstrakcije",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11409"
}

Filipović Tričković, J. G., Kokunešoski, M., Momić, T., Jovanović, I. G., Anastasov, M., Stojanović, D.,& Valenta Šobot, A.. (2023). Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book.
https://hdl.handle.net/21.15107/rcub_vinar_11409

Filipović Tričković JG, Kokunešoski M, Momić T, Jovanović IG, Anastasov M, Stojanović D, Valenta Šobot A. Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_11409 .

Filipović Tričković, Jelena G., Kokunešoski, Maja, Momić, Tatjana, Jovanović, Ivan G., Anastasov, Marina, Stojanović, Dragana, Valenta Šobot, Ana, "Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book (2023),
https://hdl.handle.net/21.15107/rcub_vinar_11409 .

TY  - CONF
AU  - Ilić, Jana
AU  - Trišić, Dijana
AU  - Čolović, Božana
AU  - Ilić, Bojana
AU  - Filipović Tričković, Jelena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12812
AB  - Aim: The use of nitinol archwires (NiTi) in orthodontic treatment has expanded significantly due to unique
mechanical properties such as shape memory effect and superelasticity. The greatest barrier to safe
application of NiTi archwires is microbiologically or electrochemically induced corrosion. Corrosion of NiTi
archwires result in Ni2+ release, leading to different health implications. The purpose of this investigation is
the improvement of NiTi archwires properties by synthesis of multilayerd, firmly bonded nanocoatings with
antibacterial properties using the magnetron sputtering (MS). These nanocoatings will enhance corrosion
resistance and decrease cytotoxic properties by preventing Ni2+ release, without compromising mechanical
characteristics of the conventional NiTi archwires. Materials and Method: The nanocoatings of TiO2, TiN,
TiN+Cu were acquired by pulsed magnetron sputtering using the commercially available nickel-titanium
archwires. The samples were assessed using atomic force microscopy and X-ray diffraction. Inductively
coupled plasma - optical emission spectrometry was used to estimate the release of Ni, Ti, Cu, Fe, Cr, Zn
ions into DMEM and acidic solution. Biocompatibility of samples' eluates was investigated using Neutral red
staining. Disk Diffusion Method was used for assessing the antibacterial properties against bacterial
suspensions of Streptococcus mutans. Results: Compared to NiTi and stainless steel, the nonocoated
archwires showed statistically lower release of nickel both in DMEM and acidic environment (p<0.05).
Regarding the relative cell viability, the 7-day eluates of nanocoated archwires was the highest (p<0.05).
Antibacterial activity of nanocoated archwires was statistically significant in comparison to the conventional
NiTi and stainless steel archwires (p<0.05). Conclusions: As nanocoated archwires presented desired
antibacterial properties with better stability in both acidic and non-acidic conditions, as well as satisfactory biocompatibility, they may be a good candidate for further clinical investigations.
PB  - European Orthodontic Society
C3  - European Journal of Orthodontics
T1  - Novel Nanocoated Nitinol Archwires With Antibacterial Properties
VL  - 45
IS  - 6 - EOS2023 Abstracts
SP  - e162
EP  - e162
DO  - 10.1093/ejo/cjad072
ER  - 

@conference{
author = "Ilić, Jana and Trišić, Dijana and Čolović, Božana and Ilić, Bojana and Filipović Tričković, Jelena",
year = "2023",
abstract = "Aim: The use of nitinol archwires (NiTi) in orthodontic treatment has expanded significantly due to unique
mechanical properties such as shape memory effect and superelasticity. The greatest barrier to safe
application of NiTi archwires is microbiologically or electrochemically induced corrosion. Corrosion of NiTi
archwires result in Ni2+ release, leading to different health implications. The purpose of this investigation is
the improvement of NiTi archwires properties by synthesis of multilayerd, firmly bonded nanocoatings with
antibacterial properties using the magnetron sputtering (MS). These nanocoatings will enhance corrosion
resistance and decrease cytotoxic properties by preventing Ni2+ release, without compromising mechanical
characteristics of the conventional NiTi archwires. Materials and Method: The nanocoatings of TiO2, TiN,
TiN+Cu were acquired by pulsed magnetron sputtering using the commercially available nickel-titanium
archwires. The samples were assessed using atomic force microscopy and X-ray diffraction. Inductively
coupled plasma - optical emission spectrometry was used to estimate the release of Ni, Ti, Cu, Fe, Cr, Zn
ions into DMEM and acidic solution. Biocompatibility of samples' eluates was investigated using Neutral red
staining. Disk Diffusion Method was used for assessing the antibacterial properties against bacterial
suspensions of Streptococcus mutans. Results: Compared to NiTi and stainless steel, the nonocoated
archwires showed statistically lower release of nickel both in DMEM and acidic environment (p<0.05).
Regarding the relative cell viability, the 7-day eluates of nanocoated archwires was the highest (p<0.05).
Antibacterial activity of nanocoated archwires was statistically significant in comparison to the conventional
NiTi and stainless steel archwires (p<0.05). Conclusions: As nanocoated archwires presented desired
antibacterial properties with better stability in both acidic and non-acidic conditions, as well as satisfactory biocompatibility, they may be a good candidate for further clinical investigations.",
publisher = "European Orthodontic Society",
journal = "European Journal of Orthodontics",
title = "Novel Nanocoated Nitinol Archwires With Antibacterial Properties",
volume = "45",
number = "6 - EOS2023 Abstracts",
pages = "e162-e162",
doi = "10.1093/ejo/cjad072"
}

Ilić, J., Trišić, D., Čolović, B., Ilić, B.,& Filipović Tričković, J.. (2023). Novel Nanocoated Nitinol Archwires With Antibacterial Properties. in European Journal of Orthodontics
European Orthodontic Society., 45(6 - EOS2023 Abstracts), e162-e162.
https://doi.org/10.1093/ejo/cjad072

Ilić J, Trišić D, Čolović B, Ilić B, Filipović Tričković J. Novel Nanocoated Nitinol Archwires With Antibacterial Properties. in European Journal of Orthodontics. 2023;45(6 - EOS2023 Abstracts):e162-e162.
doi:10.1093/ejo/cjad072 .

Ilić, Jana, Trišić, Dijana, Čolović, Božana, Ilić, Bojana, Filipović Tričković, Jelena, "Novel Nanocoated Nitinol Archwires With Antibacterial Properties" in European Journal of Orthodontics, 45, no. 6 - EOS2023 Abstracts (2023):e162-e162,
https://doi.org/10.1093/ejo/cjad072 . .

TY  - CONF
AU  - Ilić, Bojana
AU  - Petrović, Božana
AU  - Marinković, Jelena
AU  - Filipović Tričković, Jelena
AU  - Miletić Vukajlović, Jadranka
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12728
AB  - Objectives: The main purpose of orthodontic treatment (OT) is to accomplish an optimal occlusal relationship in order to obtain adequate oral function and aesthetic appearance. Under optimal economic conditions, demand for OT reaches at least 35%, but in higher socioeconomic areas in US more than 50% of children are receiving orthodontic care. Acceptance of OT in Europe, like in western population and Scandinavian countries, is at similar levels. Very desirable mechanical characteristics, such as shape memory effect and superelasticity, expanded the use of nitinol (NiTi) archwires in orthodontics significantly. The complex conditions present in the oral cavity, including biofilm formation on the exposed surfaces, substantially alter the surface and structural properties of the NiTi archwires compromising the safety of OT. The necessity for developing novel material coating that would decrease Ni release and improve biological properties is of a great importance. Materials-Methods: Copper doped titanium nitride films (TiN-Cu) on the surface of NiTi archwires were obtained by combination of the cathodic arc evaporation and DC magnetron sputtering. The physicochemical characterization was performed using energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). Ion release was assessed using inductively coupled plasma optical emission spectrometry (ICP-OES). The cytotoxicity of NiTi archwires, stainless steel (SS) archwires and TiN-Cu coated archwires was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) test. In order to evaluate bacterial adhesion and biofilm formation, the following strains were used: Streptococcus mutans and Streptococcus mitis. Results: Physicochemical characterization revealed well-designed coatings with the presence of TiN phase with incorporated Cu. The release of Ni was the lowest regarding TiN-Cunanocoated archwires (p < 0.05) and increased in acidic conditions, while the release of Ti was constant. Contrary, the release of Cu was higher in neutral conditions and decreased during the observation time (p < 0.05). The cytotoxicity was the lowest in 28-day eluates of TiN-Cu-nanocoated archwires (p < 0.05). The coating inhibited the adhesion and growth of bacteria such as Streptococcus mitis and Streptococcus mutans (p < 0.05). Conclusion: Taking into account the results of cytotoxicity test and biofilm formation, TiN-Cunanocoated archwires may be considered as a good candidate for further clinical investigations.
C3  - BaSS : 27th  International Balkan Congress Congress - “Past, Present and Future of Dental implants and Gerodontology“ : Book of abstracts
T1  - Biological properties of nitinol archwires coated with titanium nitride- copper films
SP  - 87
EP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12728
ER  - 

@conference{
author = "Ilić, Bojana and Petrović, Božana and Marinković, Jelena and Filipović Tričković, Jelena and Miletić Vukajlović, Jadranka",
year = "2023",
abstract = "Objectives: The main purpose of orthodontic treatment (OT) is to accomplish an optimal occlusal relationship in order to obtain adequate oral function and aesthetic appearance. Under optimal economic conditions, demand for OT reaches at least 35%, but in higher socioeconomic areas in US more than 50% of children are receiving orthodontic care. Acceptance of OT in Europe, like in western population and Scandinavian countries, is at similar levels. Very desirable mechanical characteristics, such as shape memory effect and superelasticity, expanded the use of nitinol (NiTi) archwires in orthodontics significantly. The complex conditions present in the oral cavity, including biofilm formation on the exposed surfaces, substantially alter the surface and structural properties of the NiTi archwires compromising the safety of OT. The necessity for developing novel material coating that would decrease Ni release and improve biological properties is of a great importance. Materials-Methods: Copper doped titanium nitride films (TiN-Cu) on the surface of NiTi archwires were obtained by combination of the cathodic arc evaporation and DC magnetron sputtering. The physicochemical characterization was performed using energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). Ion release was assessed using inductively coupled plasma optical emission spectrometry (ICP-OES). The cytotoxicity of NiTi archwires, stainless steel (SS) archwires and TiN-Cu coated archwires was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) test. In order to evaluate bacterial adhesion and biofilm formation, the following strains were used: Streptococcus mutans and Streptococcus mitis. Results: Physicochemical characterization revealed well-designed coatings with the presence of TiN phase with incorporated Cu. The release of Ni was the lowest regarding TiN-Cunanocoated archwires (p < 0.05) and increased in acidic conditions, while the release of Ti was constant. Contrary, the release of Cu was higher in neutral conditions and decreased during the observation time (p < 0.05). The cytotoxicity was the lowest in 28-day eluates of TiN-Cu-nanocoated archwires (p < 0.05). The coating inhibited the adhesion and growth of bacteria such as Streptococcus mitis and Streptococcus mutans (p < 0.05). Conclusion: Taking into account the results of cytotoxicity test and biofilm formation, TiN-Cunanocoated archwires may be considered as a good candidate for further clinical investigations.",
journal = "BaSS : 27th  International Balkan Congress Congress - “Past, Present and Future of Dental implants and Gerodontology“ : Book of abstracts",
title = "Biological properties of nitinol archwires coated with titanium nitride- copper films",
pages = "87-87",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12728"
}

Ilić, B., Petrović, B., Marinković, J., Filipović Tričković, J.,& Miletić Vukajlović, J.. (2023). Biological properties of nitinol archwires coated with titanium nitride- copper films. in BaSS : 27th  International Balkan Congress Congress - “Past, Present and Future of Dental implants and Gerodontology“ : Book of abstracts, 87-87.
https://hdl.handle.net/21.15107/rcub_vinar_12728

Ilić B, Petrović B, Marinković J, Filipović Tričković J, Miletić Vukajlović J. Biological properties of nitinol archwires coated with titanium nitride- copper films. in BaSS : 27th  International Balkan Congress Congress - “Past, Present and Future of Dental implants and Gerodontology“ : Book of abstracts. 2023;:87-87.
https://hdl.handle.net/21.15107/rcub_vinar_12728 .

Ilić, Bojana, Petrović, Božana, Marinković, Jelena, Filipović Tričković, Jelena, Miletić Vukajlović, Jadranka, "Biological properties of nitinol archwires coated with titanium nitride- copper films" in BaSS : 27th  International Balkan Congress Congress - “Past, Present and Future of Dental implants and Gerodontology“ : Book of abstracts (2023):87-87,
https://hdl.handle.net/21.15107/rcub_vinar_12728 .

TY  - CONF
AU  - Popović, I.
AU  - Valenta Šobot, Ana
AU  - Filipović Tričković, Jelena
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Ralić, V.
AU  - Abu el Rub, Anamarija
AU  - Algarra, M.
AU  - Petković, M.
AU  - Stepić, Milutin
AU  - Nešić, M.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11808
AB  - In recent years researchers have developed new strategies to enhance the effectiveness of wound healing by combining nanoparticles and infra red (IR) light. For example, some studies have shown that nanoparticles can be used to enhance the absorption of near-infrared laser (NIR) light by tissues, leading to increased healing rates [1]. The influence of NIR light on proliferation, collagen production, and wound healing was tested on: keratocytes (HaCaT) and fibroblasts (MRC-5) cells that are used as model systems of human skin equivalents that comprise an epidermal and a dermal compartment of skin. Also, these cells were treated with carbon quantum dots/silver-based metal-organic framework composites (Ag-MoFs-NCDs and Ag-MoFs-SCDs), which previously showed high antibacterial activity [2], without and with laser light. Firstly, we have found the most convenient and effective CW laser intensity (16 mW/cm2) and illumination time (3 minutes), which is not too high and short enough to influence human cells' proliferation and metabolism positively. Additional chemical treatment with Ag-MoFs-NCDs and Ag-MoFs-SCDs results in a further increase in human cell viability. Our measurements showed that the proliferation index in laser-illuminated cells and cells treated with Ag-MoFs-SCDs was at the level of the untreated control. Furthermore, Ag-MoFs-SCDs treatment and laser illumination induced a mild, insignificant increase in cellular proliferation. On the other hand, Ag-MoFs-NCDs treatment led to a more pronounced, albeit not significant increase, in cellular proliferation, while Ag-MoFs-NCDs treatment combined with laser illumination significantly increased proliferation. Also, we have detected a mild change in collagen level estimated by hydroxyproline assay, which may indicate a positive outcome of combined laser illumination and treatment, taking into account that after 48 hours, a change in cell's response to the treatment could be noticed. Finally, based on migration assay, we observe a complete wound closure after 48 hours in fibroblast cells treated with Ag-MoFs-NCDs and near-infrared laser light, Fig. 1.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
T1  - Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing
SP  - 78
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11808
ER  - 

@conference{
author = "Popović, I. and Valenta Šobot, Ana and Filipović Tričković, Jelena and Korićanac, Lela and Žakula, Jelena and Ralić, V. and Abu el Rub, Anamarija and Algarra, M. and Petković, M. and Stepić, Milutin and Nešić, M.",
year = "2023",
abstract = "In recent years researchers have developed new strategies to enhance the effectiveness of wound healing by combining nanoparticles and infra red (IR) light. For example, some studies have shown that nanoparticles can be used to enhance the absorption of near-infrared laser (NIR) light by tissues, leading to increased healing rates [1]. The influence of NIR light on proliferation, collagen production, and wound healing was tested on: keratocytes (HaCaT) and fibroblasts (MRC-5) cells that are used as model systems of human skin equivalents that comprise an epidermal and a dermal compartment of skin. Also, these cells were treated with carbon quantum dots/silver-based metal-organic framework composites (Ag-MoFs-NCDs and Ag-MoFs-SCDs), which previously showed high antibacterial activity [2], without and with laser light. Firstly, we have found the most convenient and effective CW laser intensity (16 mW/cm2) and illumination time (3 minutes), which is not too high and short enough to influence human cells' proliferation and metabolism positively. Additional chemical treatment with Ag-MoFs-NCDs and Ag-MoFs-SCDs results in a further increase in human cell viability. Our measurements showed that the proliferation index in laser-illuminated cells and cells treated with Ag-MoFs-SCDs was at the level of the untreated control. Furthermore, Ag-MoFs-SCDs treatment and laser illumination induced a mild, insignificant increase in cellular proliferation. On the other hand, Ag-MoFs-NCDs treatment led to a more pronounced, albeit not significant increase, in cellular proliferation, while Ag-MoFs-NCDs treatment combined with laser illumination significantly increased proliferation. Also, we have detected a mild change in collagen level estimated by hydroxyproline assay, which may indicate a positive outcome of combined laser illumination and treatment, taking into account that after 48 hours, a change in cell's response to the treatment could be noticed. Finally, based on migration assay, we observe a complete wound closure after 48 hours in fibroblast cells treated with Ag-MoFs-NCDs and near-infrared laser light, Fig. 1.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade",
title = "Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing",
pages = "78-78",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11808"
}

Popović, I., Valenta Šobot, A., Filipović Tričković, J., Korićanac, L., Žakula, J., Ralić, V., Abu el Rub, A., Algarra, M., Petković, M., Stepić, M.,& Nešić, M.. (2023). Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
Belgrade : Vinča Institute of Nuclear Sciences., 78-78.
https://hdl.handle.net/21.15107/rcub_vinar_11808

Popović I, Valenta Šobot A, Filipović Tričković J, Korićanac L, Žakula J, Ralić V, Abu el Rub A, Algarra M, Petković M, Stepić M, Nešić M. Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade. 2023;:78-78.
https://hdl.handle.net/21.15107/rcub_vinar_11808 .

Popović, I., Valenta Šobot, Ana, Filipović Tričković, Jelena, Korićanac, Lela, Žakula, Jelena, Ralić, V., Abu el Rub, Anamarija, Algarra, M., Petković, M., Stepić, Milutin, Nešić, M., "Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing" in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade (2023):78-78,
https://hdl.handle.net/21.15107/rcub_vinar_11808 .

TY  - CONF
AU  - Nešić, M. D.
AU  - Filipović Tričković, Jelena
AU  - Valenta Šobot, Ana
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Popović, I.
AU  - Soto, J.
AU  - Algarra, M.
AU  - Ralić, V.
AU  - Abu el Rub, Anamarija
AU  - Matijević, M.
AU  - Stepić, Milutin
AU  - Petković, M.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11809
AB  - Carbon Dots (CDs) are biocompatible, fluorescent, water-soluble, and stable nanoparticles with a high potential to be used for vast biomedical applications [1,2]. We explore the application of CDs produced from natural sources, black carrots, as anti-cancer and imaging agents. These nanoparticles suppress cell growth of three different cancer cell lines, cervical (HeLa), pancreatic (PANC-1), and melanoma (A375) cell lines in vitro. However, the cytotoxic effect against A375 cells stands out, with only 20% of viable cells left after treatment (Fig.1(a)), antimetastatic potential, and a selectivity index higher than two, which indicates that the efficacy against melanoma cells is significantly greater than the toxicity against non-malignant cells (MRC-5). Furthermore, after the cellular uptake, green fluorescence was visible in the cytosol of A375 cells (Fig. 1 (b)). On the other hand, the DAPI stain for DNA was visible as a blue light in the cell nucleus. Moreover, cells with a higher intensity of green fluorescence in the nucleus, Fig. 1 (c) indicated with arrows, were the cells with condensed chromatin in the mitotic phase of the cell cycle (Fig. 1 (d) and (e)), which indicates that CDs interact with chromatin and that they could be used as a marker of cells mitosis and proliferation. In summary, we have demonstrated the great anti-cancer potential of black carrot CDs, for image-guided anti-cancer therapy of melanoma that can be used to recognize cell proliferation.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
T1  - Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles
SP  - 79
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11809
ER  - 

@conference{
author = "Nešić, M. D. and Filipović Tričković, Jelena and Valenta Šobot, Ana and Žakula, Jelena and Korićanac, Lela and Popović, I. and Soto, J. and Algarra, M. and Ralić, V. and Abu el Rub, Anamarija and Matijević, M. and Stepić, Milutin and Petković, M.",
year = "2023",
abstract = "Carbon Dots (CDs) are biocompatible, fluorescent, water-soluble, and stable nanoparticles with a high potential to be used for vast biomedical applications [1,2]. We explore the application of CDs produced from natural sources, black carrots, as anti-cancer and imaging agents. These nanoparticles suppress cell growth of three different cancer cell lines, cervical (HeLa), pancreatic (PANC-1), and melanoma (A375) cell lines in vitro. However, the cytotoxic effect against A375 cells stands out, with only 20% of viable cells left after treatment (Fig.1(a)), antimetastatic potential, and a selectivity index higher than two, which indicates that the efficacy against melanoma cells is significantly greater than the toxicity against non-malignant cells (MRC-5). Furthermore, after the cellular uptake, green fluorescence was visible in the cytosol of A375 cells (Fig. 1 (b)). On the other hand, the DAPI stain for DNA was visible as a blue light in the cell nucleus. Moreover, cells with a higher intensity of green fluorescence in the nucleus, Fig. 1 (c) indicated with arrows, were the cells with condensed chromatin in the mitotic phase of the cell cycle (Fig. 1 (d) and (e)), which indicates that CDs interact with chromatin and that they could be used as a marker of cells mitosis and proliferation. In summary, we have demonstrated the great anti-cancer potential of black carrot CDs, for image-guided anti-cancer therapy of melanoma that can be used to recognize cell proliferation.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade",
title = "Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles",
pages = "79-79",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11809"
}

Nešić, M. D., Filipović Tričković, J., Valenta Šobot, A., Žakula, J., Korićanac, L., Popović, I., Soto, J., Algarra, M., Ralić, V., Abu el Rub, A., Matijević, M., Stepić, M.,& Petković, M.. (2023). Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
Belgrade : Vinča Institute of Nuclear Sciences., 79-79.
https://hdl.handle.net/21.15107/rcub_vinar_11809

Nešić MD, Filipović Tričković J, Valenta Šobot A, Žakula J, Korićanac L, Popović I, Soto J, Algarra M, Ralić V, Abu el Rub A, Matijević M, Stepić M, Petković M. Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade. 2023;:79-79.
https://hdl.handle.net/21.15107/rcub_vinar_11809 .

Nešić, M. D., Filipović Tričković, Jelena, Valenta Šobot, Ana, Žakula, Jelena, Korićanac, Lela, Popović, I., Soto, J., Algarra, M., Ralić, V., Abu el Rub, Anamarija, Matijević, M., Stepić, Milutin, Petković, M., "Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles" in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade (2023):79-79,
https://hdl.handle.net/21.15107/rcub_vinar_11809 .

TY  - CONF
AU  - Valenta Šobot, Ana
AU  - Drakulić, Dunja
AU  - Kokunešoski, Maja
AU  - Momić, Tatjana
AU  - Filipović Tričković, Jelena G.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11410
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book
T1  - Loganic acid induces apoptosis in human peripheral blood mononuclear cells
T1  - Loganska kiselina indukuje apoptozu u mononuklearnim ćelijama periferne krvi čoveka
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11410
ER  - 

@conference{
author = "Valenta Šobot, Ana and Drakulić, Dunja and Kokunešoski, Maja and Momić, Tatjana and Filipović Tričković, Jelena G.",
year = "2023",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book",
title = "Loganic acid induces apoptosis in human peripheral blood mononuclear cells, Loganska kiselina indukuje apoptozu u mononuklearnim ćelijama periferne krvi čoveka",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11410"
}

Valenta Šobot, A., Drakulić, D., Kokunešoski, M., Momić, T.,& Filipović Tričković, J. G.. (2023). Loganic acid induces apoptosis in human peripheral blood mononuclear cells. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book.
https://hdl.handle.net/21.15107/rcub_vinar_11410

Valenta Šobot A, Drakulić D, Kokunešoski M, Momić T, Filipović Tričković JG. Loganic acid induces apoptosis in human peripheral blood mononuclear cells. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_11410 .

Valenta Šobot, Ana, Drakulić, Dunja, Kokunešoski, Maja, Momić, Tatjana, Filipović Tričković, Jelena G., "Loganic acid induces apoptosis in human peripheral blood mononuclear cells" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book (2023),
https://hdl.handle.net/21.15107/rcub_vinar_11410 .

TY  - CONF
AU  - Životić, Ivan
AU  - Kolić, Ivana
AU  - Popić, Kristina
AU  - Filipović Tričković, Jelena G.
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan G.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10831
AB  - Introduction: Genetic studies of congenital anomalies of the kidney and urinary tract (CAKUT) have demonstrated variable penetrability and expressivity of the associated genetic defects. Previously, it was shown that deletions of 17q12 and 22q11.2 regions were specific for kidney anomalies (KA) while 16p11.2 and 1q21.1 loci showed extensive pleiotropy in CAKUT phenotypes. CNVs affecting miRNA gene dosage have been described to have functional influence on gene expression. We aimed to conduct comprehensive in silico analysis using publicly available databases to analyze miRNA content of CAKUT-associated CNVs in quoted chromosomal loci with regard to pleiotropy.  Methods: Extensive literature review was conducted to collect data about pathogenic rCNVs associated with CAKUT. UCSC genome browser tool was employed for mapping miRNAs onto collected rCNV regions.  Results: Analysis of CNVs in CAKUT included four studies counting more than 2500 patients. In further analysis we included 191 patients harboring pathogenic CNVs. Surprisingly, CAKUT pleiotropic regions (16p11.2, 1q21.2) did not contain any miRNA. 22q11.2 showed the densest miRNAs content (n = 21).  Conclusions: Absence of miRNAs may potentially pronounce the pleiotropy of the CAKUT genetic defects, thus leading to the variety of phenotypes. Contrary, abundancy of miRNAs in 22q11.2 might be associated with reproducible phenotype, such as KA, producing the functional effect when deleted. This assumption agrees with recent results of miRNA expression variability in 22q11.2 deletion syndrome.
C3  - European Journal of Human Genetics
T1  - miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes
VL  - 30
IS  - Suppl. 1
SP  - 331
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10831
ER  - 

@conference{
author = "Životić, Ivan and Kolić, Ivana and Popić, Kristina and Filipović Tričković, Jelena G. and Đorđević, Ana and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan G.",
year = "2022",
abstract = "Introduction: Genetic studies of congenital anomalies of the kidney and urinary tract (CAKUT) have demonstrated variable penetrability and expressivity of the associated genetic defects. Previously, it was shown that deletions of 17q12 and 22q11.2 regions were specific for kidney anomalies (KA) while 16p11.2 and 1q21.1 loci showed extensive pleiotropy in CAKUT phenotypes. CNVs affecting miRNA gene dosage have been described to have functional influence on gene expression. We aimed to conduct comprehensive in silico analysis using publicly available databases to analyze miRNA content of CAKUT-associated CNVs in quoted chromosomal loci with regard to pleiotropy.  Methods: Extensive literature review was conducted to collect data about pathogenic rCNVs associated with CAKUT. UCSC genome browser tool was employed for mapping miRNAs onto collected rCNV regions.  Results: Analysis of CNVs in CAKUT included four studies counting more than 2500 patients. In further analysis we included 191 patients harboring pathogenic CNVs. Surprisingly, CAKUT pleiotropic regions (16p11.2, 1q21.2) did not contain any miRNA. 22q11.2 showed the densest miRNAs content (n = 21).  Conclusions: Absence of miRNAs may potentially pronounce the pleiotropy of the CAKUT genetic defects, thus leading to the variety of phenotypes. Contrary, abundancy of miRNAs in 22q11.2 might be associated with reproducible phenotype, such as KA, producing the functional effect when deleted. This assumption agrees with recent results of miRNA expression variability in 22q11.2 deletion syndrome.",
journal = "European Journal of Human Genetics",
title = "miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes",
volume = "30",
number = "Suppl. 1",
pages = "331",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10831"
}

Životić, I., Kolić, I., Popić, K., Filipović Tričković, J. G., Đorđević, A., Živković, M., Stanković, A.,& Jovanović, I. G.. (2022). miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes. in European Journal of Human Genetics, 30(Suppl. 1), 331.
https://hdl.handle.net/21.15107/rcub_vinar_10831

Životić I, Kolić I, Popić K, Filipović Tričković JG, Đorđević A, Živković M, Stanković A, Jovanović IG. miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes. in European Journal of Human Genetics. 2022;30(Suppl. 1):331.
https://hdl.handle.net/21.15107/rcub_vinar_10831 .

Životić, Ivan, Kolić, Ivana, Popić, Kristina, Filipović Tričković, Jelena G., Đorđević, Ana, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan G., "miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes" in European Journal of Human Genetics, 30, no. Suppl. 1 (2022):331,
https://hdl.handle.net/21.15107/rcub_vinar_10831 .

TY  - CONF
AU  - Mitrović, Kristina
AU  - Kolić, Ivana
AU  - Životić, Ivan
AU  - Filipović Tričković, Jelena G.
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan G.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10830
AB  - Introduction: miR-548 family members, located on all human chromosomes except chr19 and chrY, regulate podocyte differentiation in vitro, important for kidney development. Rare copy number variants (rCNVs) are the common genetic cause of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT) and could harbour miRNAs. The aim of this study was to investigate to which extent rCNVs associated with CAKUT harbour miR-548 members.
Materials and Methods: Extensive literature review was conducted to collect data of pathogenic and likely pathogenic rCNVs in CAKUT patients. UCSC genome browser tool was employed for mapping of miR-548 members onto collected rCNV regions and gnomAD SV controls database. Bioinformatic analysis was conducted using miRPathDB2 tool.
Results: We generated CAKUT database of pathogenic CNVs in 79 chromosome regions from 191 patient and likely pathogenic CNVs in 74 regions from 87 patients. Pathogenic rCNVs of seventeen patients, located on 7 chromosomes, contained at least one miR-548 member. Likely pathogenic rCNVs of 4 patients, located on 3 chromosomes, contained one of miR-548 members. Bioinformatic analysis implied the role of mapped miRNAs in the regulation of processes associated with CAKUT. In controls, only hsa-mir-548i-3 (out of 73 precursors) was mapped on polymorphic CNVs (af>1%) and wasn’t identified in patients.
Conclusions: miR-548 members located in rCNVs should be investigated in future studies as potential genetic drivers of CAKUT development, beyond protein coding genes.
C3  - European Journal of Human Genetics
T1  - Are miR-548 family members potential genetic drivers of CAKUT
VL  - 30
IS  - Suppl. 1
SP  - 331
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10830
ER  - 

@conference{
author = "Mitrović, Kristina and Kolić, Ivana and Životić, Ivan and Filipović Tričković, Jelena G. and Đorđević, Ana and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan G.",
year = "2022",
abstract = "Introduction: miR-548 family members, located on all human chromosomes except chr19 and chrY, regulate podocyte differentiation in vitro, important for kidney development. Rare copy number variants (rCNVs) are the common genetic cause of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT) and could harbour miRNAs. The aim of this study was to investigate to which extent rCNVs associated with CAKUT harbour miR-548 members.
Materials and Methods: Extensive literature review was conducted to collect data of pathogenic and likely pathogenic rCNVs in CAKUT patients. UCSC genome browser tool was employed for mapping of miR-548 members onto collected rCNV regions and gnomAD SV controls database. Bioinformatic analysis was conducted using miRPathDB2 tool.
Results: We generated CAKUT database of pathogenic CNVs in 79 chromosome regions from 191 patient and likely pathogenic CNVs in 74 regions from 87 patients. Pathogenic rCNVs of seventeen patients, located on 7 chromosomes, contained at least one miR-548 member. Likely pathogenic rCNVs of 4 patients, located on 3 chromosomes, contained one of miR-548 members. Bioinformatic analysis implied the role of mapped miRNAs in the regulation of processes associated with CAKUT. In controls, only hsa-mir-548i-3 (out of 73 precursors) was mapped on polymorphic CNVs (af>1%) and wasn’t identified in patients.
Conclusions: miR-548 members located in rCNVs should be investigated in future studies as potential genetic drivers of CAKUT development, beyond protein coding genes.",
journal = "European Journal of Human Genetics",
title = "Are miR-548 family members potential genetic drivers of CAKUT",
volume = "30",
number = "Suppl. 1",
pages = "331",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10830"
}

Mitrović, K., Kolić, I., Životić, I., Filipović Tričković, J. G., Đorđević, A., Živković, M., Stanković, A.,& Jovanović, I. G.. (2022). Are miR-548 family members potential genetic drivers of CAKUT. in European Journal of Human Genetics, 30(Suppl. 1), 331.
https://hdl.handle.net/21.15107/rcub_vinar_10830

Mitrović K, Kolić I, Životić I, Filipović Tričković JG, Đorđević A, Živković M, Stanković A, Jovanović IG. Are miR-548 family members potential genetic drivers of CAKUT. in European Journal of Human Genetics. 2022;30(Suppl. 1):331.
https://hdl.handle.net/21.15107/rcub_vinar_10830 .

Mitrović, Kristina, Kolić, Ivana, Životić, Ivan, Filipović Tričković, Jelena G., Đorđević, Ana, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan G., "Are miR-548 family members potential genetic drivers of CAKUT" in European Journal of Human Genetics, 30, no. Suppl. 1 (2022):331,
https://hdl.handle.net/21.15107/rcub_vinar_10830 .

TY  - JOUR
AU  - Mitrović, Kristina
AU  - Životić, Ivan
AU  - Kolić, Ivana
AU  - Đorđević, Ana
AU  - Žakula, Jelena
AU  - Filipović Tričković, Jelena G.
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan G.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10469
AB  - Rare copy number variants (CNVs) are among the most common genomic disorders underlying CAKUT. miRNAs located in rare CNVs represent well-founded functional variants for human CAKUT research. The study aimed to identify and functionally interpret miRNAs most frequently affected by rare CNVs in CAKUT and to estimate the overall burden of rare CNVs on miRNA genes in CAKUT. The additional aim of this study was to experimentally confirm the effect of a rare CNV in CAKUT on candidate miRNA’s expression and the subsequent change in mRNA levels of selected target genes. A database of CAKUT-associated rare CNV regions, created by literature mining, was used for mapping of the miRNA precursors. miRNAs and miRNA families, most frequently affected by rare CAKUT-associated CNVs, have been subjected to bioinformatic analysis. CNV burden analysis was performed to identify chromosomes with over/underrepresentation of miRNA genes in rare CNVs associated with CAKUT. A functional study was performed on HEK293 MIR484+/- KO and HEK293 WT cell lines, followed by the analysis of relative miRNA and mRNA target gene levels. 80% of CAKUT patients with underlying rare CNV had at least one miRNA gene overlapping the identified CNV. Network analysis of the most frequently affected miRNAs has revealed the dominant regulation of the two miRNAs, hsa-miR-484 and hsa-miR-185-5p. Additionally, miR-548 family members have shown substantial enrichment in rare CNVs in CAKUT. An over/underrepresentation of miRNA genes in rare CNVs associated with CAKUT was observed in multiple chromosomes, such as chr16, chr20, and chr21. A significant 0.37 fold downregulation of hsa-miR-484, followed by a notable upregulation of MDM2 and APAF1 and downregulation of NOTCH3 was detected in HEK293 MIR484+/- KO compared to HEK293 WT cell lines, supporting the study hypothesis. miRNA genes are frequently affected by rare CNVs in CAKUT patients. Understanding the potential of CNV-affected miRNAs to participate in CAKUT as genetic drivers represent a crucial implication for the development of novel therapeutic approaches.
T2  - Scientific Reports
T1  - Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT
VL  - 12
IS  - 1
SP  - 17746
DO  - 10.1038/s41598-022-22749-1
ER  - 

@article{
author = "Mitrović, Kristina and Životić, Ivan and Kolić, Ivana and Đorđević, Ana and Žakula, Jelena and Filipović Tričković, Jelena G. and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan G.",
year = "2022",
abstract = "Rare copy number variants (CNVs) are among the most common genomic disorders underlying CAKUT. miRNAs located in rare CNVs represent well-founded functional variants for human CAKUT research. The study aimed to identify and functionally interpret miRNAs most frequently affected by rare CNVs in CAKUT and to estimate the overall burden of rare CNVs on miRNA genes in CAKUT. The additional aim of this study was to experimentally confirm the effect of a rare CNV in CAKUT on candidate miRNA’s expression and the subsequent change in mRNA levels of selected target genes. A database of CAKUT-associated rare CNV regions, created by literature mining, was used for mapping of the miRNA precursors. miRNAs and miRNA families, most frequently affected by rare CAKUT-associated CNVs, have been subjected to bioinformatic analysis. CNV burden analysis was performed to identify chromosomes with over/underrepresentation of miRNA genes in rare CNVs associated with CAKUT. A functional study was performed on HEK293 MIR484+/- KO and HEK293 WT cell lines, followed by the analysis of relative miRNA and mRNA target gene levels. 80% of CAKUT patients with underlying rare CNV had at least one miRNA gene overlapping the identified CNV. Network analysis of the most frequently affected miRNAs has revealed the dominant regulation of the two miRNAs, hsa-miR-484 and hsa-miR-185-5p. Additionally, miR-548 family members have shown substantial enrichment in rare CNVs in CAKUT. An over/underrepresentation of miRNA genes in rare CNVs associated with CAKUT was observed in multiple chromosomes, such as chr16, chr20, and chr21. A significant 0.37 fold downregulation of hsa-miR-484, followed by a notable upregulation of MDM2 and APAF1 and downregulation of NOTCH3 was detected in HEK293 MIR484+/- KO compared to HEK293 WT cell lines, supporting the study hypothesis. miRNA genes are frequently affected by rare CNVs in CAKUT patients. Understanding the potential of CNV-affected miRNAs to participate in CAKUT as genetic drivers represent a crucial implication for the development of novel therapeutic approaches.",
journal = "Scientific Reports",
title = "Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT",
volume = "12",
number = "1",
pages = "17746",
doi = "10.1038/s41598-022-22749-1"
}

Mitrović, K., Životić, I., Kolić, I., Đorđević, A., Žakula, J., Filipović Tričković, J. G., Živković, M., Stanković, A.,& Jovanović, I. G.. (2022). Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT. in Scientific Reports, 12(1), 17746.
https://doi.org/10.1038/s41598-022-22749-1

Mitrović K, Životić I, Kolić I, Đorđević A, Žakula J, Filipović Tričković JG, Živković M, Stanković A, Jovanović IG. Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT. in Scientific Reports. 2022;12(1):17746.
doi:10.1038/s41598-022-22749-1 .

Mitrović, Kristina, Životić, Ivan, Kolić, Ivana, Đorđević, Ana, Žakula, Jelena, Filipović Tričković, Jelena G., Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan G., "Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT" in Scientific Reports, 12, no. 1 (2022):17746,
https://doi.org/10.1038/s41598-022-22749-1 . .

TY  - CONF
AU  - Kolić, Ivana
AU  - Mitrović, Kristina
AU  - Životić, Ivan
AU  - Đorđević, Ana
AU  - Filipović-Tričković, Jelena
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12684
AB  - Background/Objectives: Rare copy number variants (rCNVs) are the common genetic cause of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT).1 miRNAs located in rCNVs represent well-founded functional variants for human CAKUT research. However, the impact of rCNVs on miRNA genes in CAKUT is unknown. Thus, burden assessment was performed to identify chromosomes with non-random representation of miRNA genes in rCNVs associated with CAKUT. Methods: A comprehensive literature mining of rCNV regions associated with CAKUT was performed. The total cumulative length of rCNVs per chromosome was the sum of corresponding CNV-DNA regions, taking into account overlapping. Mapping of miRNAs onto cumulative rCNV regions gave counts of affected miRNA loci. The correlation analysis was performed between the number of miRNA genes overlapping rCNVs, and the fractional lengths of cumulative rCNVs regions in relation to the chromosome size. Results: A statistically significant positive correlation was observed for duplications and deletions respectively (Spearman correlation p<0.0001, r=0.9, r=0.8). However, a deviation from the best fit line for chromosome 16, for both rare duplications and deletions, was observed due to the high overrepresentation of miRNA genes in identified rCNVs. Conclusion: The current finding of the high overall burden of rCNVs on miRNA genes in chromosome 16 suggests that miRNAs located on this chromosome could serve as candidates for the investigation of miRNA role in CAKUT development.
C3  - 54th European Society of Human Genetics (ESHG) : Book of abstracts
T1  - Assessing the burden of rare CNVs on miRNA genes in CAKUT
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12684
ER  - 

@conference{
author = "Kolić, Ivana and Mitrović, Kristina and Životić, Ivan and Đorđević, Ana and Filipović-Tričković, Jelena and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan",
year = "2022",
abstract = "Background/Objectives: Rare copy number variants (rCNVs) are the common genetic cause of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT).1 miRNAs located in rCNVs represent well-founded functional variants for human CAKUT research. However, the impact of rCNVs on miRNA genes in CAKUT is unknown. Thus, burden assessment was performed to identify chromosomes with non-random representation of miRNA genes in rCNVs associated with CAKUT. Methods: A comprehensive literature mining of rCNV regions associated with CAKUT was performed. The total cumulative length of rCNVs per chromosome was the sum of corresponding CNV-DNA regions, taking into account overlapping. Mapping of miRNAs onto cumulative rCNV regions gave counts of affected miRNA loci. The correlation analysis was performed between the number of miRNA genes overlapping rCNVs, and the fractional lengths of cumulative rCNVs regions in relation to the chromosome size. Results: A statistically significant positive correlation was observed for duplications and deletions respectively (Spearman correlation p<0.0001, r=0.9, r=0.8). However, a deviation from the best fit line for chromosome 16, for both rare duplications and deletions, was observed due to the high overrepresentation of miRNA genes in identified rCNVs. Conclusion: The current finding of the high overall burden of rCNVs on miRNA genes in chromosome 16 suggests that miRNAs located on this chromosome could serve as candidates for the investigation of miRNA role in CAKUT development.",
journal = "54th European Society of Human Genetics (ESHG) : Book of abstracts",
title = "Assessing the burden of rare CNVs on miRNA genes in CAKUT",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12684"
}

Kolić, I., Mitrović, K., Životić, I., Đorđević, A., Filipović-Tričković, J., Živković, M., Stanković, A.,& Jovanović, I.. (2022). Assessing the burden of rare CNVs on miRNA genes in CAKUT. in 54th European Society of Human Genetics (ESHG) : Book of abstracts.
https://hdl.handle.net/21.15107/rcub_vinar_12684

Kolić I, Mitrović K, Životić I, Đorđević A, Filipović-Tričković J, Živković M, Stanković A, Jovanović I. Assessing the burden of rare CNVs on miRNA genes in CAKUT. in 54th European Society of Human Genetics (ESHG) : Book of abstracts. 2022;.
https://hdl.handle.net/21.15107/rcub_vinar_12684 .

Kolić, Ivana, Mitrović, Kristina, Životić, Ivan, Đorđević, Ana, Filipović-Tričković, Jelena, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan, "Assessing the burden of rare CNVs on miRNA genes in CAKUT" in 54th European Society of Human Genetics (ESHG) : Book of abstracts (2022),
https://hdl.handle.net/21.15107/rcub_vinar_12684 .

TY  - CONF
AU  - Valenta Šobot, Ana
AU  - Filipović Tričković, Jelena
AU  - Drakulić, Dunja
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Momić, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11582
AB  - Sweroside (Sw) is a secondary metabolite commonly found in plants belonging to the Gentianaceae family [1]. This iridoid compound is well-known for its anti-inflammatory [2], antidiabetic [3] and antitumor properties [4], which have been studied in pathological model systems. In transformed cell lines, Sw displays an antitumor effect by apoptosis activation [5]. Since healthy cells are also exposed to cancer therapy applied in vivo, our goal was to determine Sw’s cytotoxic concentration in primary human peripheral blood mononuclear cells (PBMCs) after 48 h of treatment with a range of concentration from 20 μM to 130 μM Sw in vitro, and to analyze whether the cytotoxic effect was due to activated apoptosis. According to the obtained results of the trypan blue dye exclusion test, 48 h of treatment with 50 μM Sw and higher concentrations led to a significant decrease in cell number. The DNA fragmentation assay indicated that following 50 μM Sw treatment, cells are dying in an apoptosis-like manner since the level of DNA fragments was 3.5 times higher than in the untreated control. The type of cell death was confirmed by immunoblot analysis of apoptosis- specific protein markers, which revealed the elevation of cleaved caspase-3 and PARP1 89 kDa fragments. Our findings showed that like in transformed cell lines, Sw in healthy cells can also activate apoptosis. A potential difference in sensitivity to Sw treatment between healthy and transformed cells could justify Sw treatment in anticancer therapy.
PB  - Belgrade : University of Belgrade, Faculty of Agriculture
C3  - 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
T1  - Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells
SP  - 47
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11582
ER  - 

@conference{
author = "Valenta Šobot, Ana and Filipović Tričković, Jelena and Drakulić, Dunja and Leskovac, Andreja and Petrović, Sandra and Momić, Tatjana",
year = "2022",
abstract = "Sweroside (Sw) is a secondary metabolite commonly found in plants belonging to the Gentianaceae family [1]. This iridoid compound is well-known for its anti-inflammatory [2], antidiabetic [3] and antitumor properties [4], which have been studied in pathological model systems. In transformed cell lines, Sw displays an antitumor effect by apoptosis activation [5]. Since healthy cells are also exposed to cancer therapy applied in vivo, our goal was to determine Sw’s cytotoxic concentration in primary human peripheral blood mononuclear cells (PBMCs) after 48 h of treatment with a range of concentration from 20 μM to 130 μM Sw in vitro, and to analyze whether the cytotoxic effect was due to activated apoptosis. According to the obtained results of the trypan blue dye exclusion test, 48 h of treatment with 50 μM Sw and higher concentrations led to a significant decrease in cell number. The DNA fragmentation assay indicated that following 50 μM Sw treatment, cells are dying in an apoptosis-like manner since the level of DNA fragments was 3.5 times higher than in the untreated control. The type of cell death was confirmed by immunoblot analysis of apoptosis- specific protein markers, which revealed the elevation of cleaved caspase-3 and PARP1 89 kDa fragments. Our findings showed that like in transformed cell lines, Sw in healthy cells can also activate apoptosis. A potential difference in sensitivity to Sw treatment between healthy and transformed cells could justify Sw treatment in anticancer therapy.",
publisher = "Belgrade : University of Belgrade, Faculty of Agriculture",
journal = "1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts",
title = "Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells",
pages = "47-47",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11582"
}

Valenta Šobot, A., Filipović Tričković, J., Drakulić, D., Leskovac, A., Petrović, S.,& Momić, T.. (2022). Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
Belgrade : University of Belgrade, Faculty of Agriculture., 47-47.
https://hdl.handle.net/21.15107/rcub_vinar_11582

Valenta Šobot A, Filipović Tričković J, Drakulić D, Leskovac A, Petrović S, Momić T. Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts. 2022;:47-47.
https://hdl.handle.net/21.15107/rcub_vinar_11582 .

Valenta Šobot, Ana, Filipović Tričković, Jelena, Drakulić, Dunja, Leskovac, Andreja, Petrović, Sandra, Momić, Tatjana, "Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells" in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts (2022):47-47,
https://hdl.handle.net/21.15107/rcub_vinar_11582 .

TY  - CONF
AU  - Valenta Šobot, Ana
AU  - Filipović Tričković, Jelena
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Momić, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11583
AB  - Quercetin (Q) is one of the most common and well researched antioxidant flavonoids, which usually occurs in plant-based foods, and medicinal plants. It was shown that quercetin exerts many beneficial effects on human health, including prevention of cancer and heart diseases. Quercetin was found to be toxic toward various types of cancer cell, still, at higher concentrations it was also shown toxic toward normal human cells [1,2]. One of the approaches to overcome this shortcoming offers nanotechnology which enables the novel perspective of phytochemical usage in contemporary medicine [3]. The strategy of binding quercetin to the gold nanoparticles during their synthesis was used, which resulted in quercetin capped gold nanoparticles (NPQ) [4]. Trypan blue exclusion test [5] was used to evaluate peripheral blood mononuclear cells (PBMC) viability after their exposure to either NPQ or free Q during 24, 48 and 72 h, at 37 °C, in the range of quercetin concentrations from 5 to 50 μg/mL. A significant reduction in the cell count was observed in PBMC cultures treated with 10, 20, and 50 μg/mL of free Q, for all exposure times. The treatments of increasing concentrations and exposure times lowered the cells viability, resulting in 63% of the viable cells, following 72 h of the treatment with 50 μg/mL of free Q. Although NPQ treatments affected the cells viability in a concentration- and time-dependent manner the treatment with 50 μg/mL of NPQ for 72 h, had a milder effect on PBMC cultures than free Q, resulting in 81% of the viable cells (Figure 1). According to the obtained results, NPQ were shown less toxic toward PBMC than free Q.
PB  - Belgrade : University of Belgrade, Faculty of Agriculture
C3  - 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
T1  - Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells
SP  - 64
EP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11583
ER  - 

@conference{
author = "Valenta Šobot, Ana and Filipović Tričković, Jelena and Leskovac, Andreja and Petrović, Sandra and Momić, Tatjana",
year = "2022",
abstract = "Quercetin (Q) is one of the most common and well researched antioxidant flavonoids, which usually occurs in plant-based foods, and medicinal plants. It was shown that quercetin exerts many beneficial effects on human health, including prevention of cancer and heart diseases. Quercetin was found to be toxic toward various types of cancer cell, still, at higher concentrations it was also shown toxic toward normal human cells [1,2]. One of the approaches to overcome this shortcoming offers nanotechnology which enables the novel perspective of phytochemical usage in contemporary medicine [3]. The strategy of binding quercetin to the gold nanoparticles during their synthesis was used, which resulted in quercetin capped gold nanoparticles (NPQ) [4]. Trypan blue exclusion test [5] was used to evaluate peripheral blood mononuclear cells (PBMC) viability after their exposure to either NPQ or free Q during 24, 48 and 72 h, at 37 °C, in the range of quercetin concentrations from 5 to 50 μg/mL. A significant reduction in the cell count was observed in PBMC cultures treated with 10, 20, and 50 μg/mL of free Q, for all exposure times. The treatments of increasing concentrations and exposure times lowered the cells viability, resulting in 63% of the viable cells, following 72 h of the treatment with 50 μg/mL of free Q. Although NPQ treatments affected the cells viability in a concentration- and time-dependent manner the treatment with 50 μg/mL of NPQ for 72 h, had a milder effect on PBMC cultures than free Q, resulting in 81% of the viable cells (Figure 1). According to the obtained results, NPQ were shown less toxic toward PBMC than free Q.",
publisher = "Belgrade : University of Belgrade, Faculty of Agriculture",
journal = "1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts",
title = "Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells",
pages = "64-64",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11583"
}

Valenta Šobot, A., Filipović Tričković, J., Leskovac, A., Petrović, S.,& Momić, T.. (2022). Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
Belgrade : University of Belgrade, Faculty of Agriculture., 64-64.
https://hdl.handle.net/21.15107/rcub_vinar_11583

Valenta Šobot A, Filipović Tričković J, Leskovac A, Petrović S, Momić T. Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts. 2022;:64-64.
https://hdl.handle.net/21.15107/rcub_vinar_11583 .

Valenta Šobot, Ana, Filipović Tričković, Jelena, Leskovac, Andreja, Petrović, Sandra, Momić, Tatjana, "Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells" in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts (2022):64-64,
https://hdl.handle.net/21.15107/rcub_vinar_11583 .

TY  - JOUR
AU  - Filipović Tričković, Jelena G.
AU  - Valenta-Šobot, Ana
AU  - Joksić, Ivana
AU  - Joksić, Gordana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10258
AB  - AbstractBiološki učinci ionizirajućega zračenja (IZ) pripisuju se oštećenjima DNA i indirektnim učincima kroz povećanu proizvodnju reaktivnih vrsta kisika. Iako se telomere rabe kao pokazatelji radioosjetljivosti, o njihovu ponašanju kao odgovoru na ionizirajuće zračenje u uvjetima profesionalne izloženosti i dalje se raspravlja. U ovom radu željeli smo istražiti duljinu i strukturu telomera u bolničkih radnika koji su profesionalno izloženi ionizirajućem zračenju te povezati te nalaze s oksidacijskim biomolekulama i kromosomskim aberacijama. Uzorci krvi izloženih ispitanika i zdravih kontrola uzeti su za analizu tijekom rutinskoga godišnjeg zdravstvenog pregleda. Osim kromosomskih aberacija, u uzorcima plazme izmjereni su i parametri oksidacijskoga stresa [prooksidacijska/antioksidacijska ravnoteža (PAB), lipidna peroksidacija i 8-okso-dG], a procjena duljine i strukture telomera provedena je metodom Q-FISH na metafaznim kromosomima. Analiza kromosomskih aberacija pokazala je da od 34 ispitanika njih 14 ima kromosomske aberacije (skupina 1), a 20 nije imalo aberacije (skupina 2). Nije bilo značajne razlike u spolu ili dobi ni u duljini telomera između skupina. Međutim, incidencija lomljivih telomera bila je značajno veća u objema skupinama ispitanika izloženih IZ-u u usporedbi s kontrolnim ispitanicima. Produkti peroksidacije lipida i 8-okso-dG također su bili značajno viši u objema skupinama. Učestalost lomljivih telomera u pozitivnoj je korelaciji (statistički značajna) s razinama 8-okso-dG u
T2  - Archives of Industrial Hygiene and Toxicology
T1  - Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation
VL  - 73
IS  - 1
SP  - 23
EP  - 30
DO  - 10.2478/aiht-2022-73-3609
ER  - 

@article{
author = "Filipović Tričković, Jelena G. and Valenta-Šobot, Ana and Joksić, Ivana and Joksić, Gordana",
year = "2022",
abstract = "AbstractBiološki učinci ionizirajućega zračenja (IZ) pripisuju se oštećenjima DNA i indirektnim učincima kroz povećanu proizvodnju reaktivnih vrsta kisika. Iako se telomere rabe kao pokazatelji radioosjetljivosti, o njihovu ponašanju kao odgovoru na ionizirajuće zračenje u uvjetima profesionalne izloženosti i dalje se raspravlja. U ovom radu željeli smo istražiti duljinu i strukturu telomera u bolničkih radnika koji su profesionalno izloženi ionizirajućem zračenju te povezati te nalaze s oksidacijskim biomolekulama i kromosomskim aberacijama. Uzorci krvi izloženih ispitanika i zdravih kontrola uzeti su za analizu tijekom rutinskoga godišnjeg zdravstvenog pregleda. Osim kromosomskih aberacija, u uzorcima plazme izmjereni su i parametri oksidacijskoga stresa [prooksidacijska/antioksidacijska ravnoteža (PAB), lipidna peroksidacija i 8-okso-dG], a procjena duljine i strukture telomera provedena je metodom Q-FISH na metafaznim kromosomima. Analiza kromosomskih aberacija pokazala je da od 34 ispitanika njih 14 ima kromosomske aberacije (skupina 1), a 20 nije imalo aberacije (skupina 2). Nije bilo značajne razlike u spolu ili dobi ni u duljini telomera između skupina. Međutim, incidencija lomljivih telomera bila je značajno veća u objema skupinama ispitanika izloženih IZ-u u usporedbi s kontrolnim ispitanicima. Produkti peroksidacije lipida i 8-okso-dG također su bili značajno viši u objema skupinama. Učestalost lomljivih telomera u pozitivnoj je korelaciji (statistički značajna) s razinama 8-okso-dG u",
journal = "Archives of Industrial Hygiene and Toxicology",
title = "Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation",
volume = "73",
number = "1",
pages = "23-30",
doi = "10.2478/aiht-2022-73-3609"
}

Filipović Tričković, J. G., Valenta-Šobot, A., Joksić, I.,& Joksić, G.. (2022). Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation. in Archives of Industrial Hygiene and Toxicology, 73(1), 23-30.
https://doi.org/10.2478/aiht-2022-73-3609

Filipović Tričković JG, Valenta-Šobot A, Joksić I, Joksić G. Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation. in Archives of Industrial Hygiene and Toxicology. 2022;73(1):23-30.
doi:10.2478/aiht-2022-73-3609 .

Filipović Tričković, Jelena G., Valenta-Šobot, Ana, Joksić, Ivana, Joksić, Gordana, "Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation" in Archives of Industrial Hygiene and Toxicology, 73, no. 1 (2022):23-30,
https://doi.org/10.2478/aiht-2022-73-3609 . .

TY  - JOUR
AU  - Matijević, Milica
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Radoičić, Marija B.
AU  - Liang, Xinyue
AU  - Mi, Lan
AU  - Filipović Tričković, Jelena G.
AU  - Valenta-Šobot, Ana
AU  - Stanković, Maja N.
AU  - Nakarada, Đura
AU  - Mojović, Miloš
AU  - Petković, Marijana
AU  - Stepić, Milutin
AU  - Nešić, Maja D.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9908
AB  - In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy.
T2  - Photochemical and Photobiological Sciences
T1  - Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles
VL  - 20
IS  - 8
SP  - 1087
EP  - 1098
DO  - 10.1007/s43630-021-00082-2
ER  - 

@article{
author = "Matijević, Milica and Žakula, Jelena and Korićanac, Lela and Radoičić, Marija B. and Liang, Xinyue and Mi, Lan and Filipović Tričković, Jelena G. and Valenta-Šobot, Ana and Stanković, Maja N. and Nakarada, Đura and Mojović, Miloš and Petković, Marijana and Stepić, Milutin and Nešić, Maja D.",
year = "2021",
abstract = "In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy.",
journal = "Photochemical and Photobiological Sciences",
title = "Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles",
volume = "20",
number = "8",
pages = "1087-1098",
doi = "10.1007/s43630-021-00082-2"
}

Matijević, M., Žakula, J., Korićanac, L., Radoičić, M. B., Liang, X., Mi, L., Filipović Tričković, J. G., Valenta-Šobot, A., Stanković, M. N., Nakarada, Đ., Mojović, M., Petković, M., Stepić, M.,& Nešić, M. D.. (2021). Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles. in Photochemical and Photobiological Sciences, 20(8), 1087-1098.
https://doi.org/10.1007/s43630-021-00082-2

Matijević M, Žakula J, Korićanac L, Radoičić MB, Liang X, Mi L, Filipović Tričković JG, Valenta-Šobot A, Stanković MN, Nakarada Đ, Mojović M, Petković M, Stepić M, Nešić MD. Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles. in Photochemical and Photobiological Sciences. 2021;20(8):1087-1098.
doi:10.1007/s43630-021-00082-2 .

Matijević, Milica, Žakula, Jelena, Korićanac, Lela, Radoičić, Marija B., Liang, Xinyue, Mi, Lan, Filipović Tričković, Jelena G., Valenta-Šobot, Ana, Stanković, Maja N., Nakarada, Đura, Mojović, Miloš, Petković, Marijana, Stepić, Milutin, Nešić, Maja D., "Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles" in Photochemical and Photobiological Sciences, 20, no. 8 (2021):1087-1098,
https://doi.org/10.1007/s43630-021-00082-2 . .

TY  - CONF
AU  - Valenta-Šobot, Ana
AU  - Filipović Tričković, Jelena G.
AU  - Šaponjić, Aleksandra
AU  - Ilić, Svetlana
AU  - Grce, Ana
AU  - Katnić, Đurica
AU  - Kokunešoski, Maja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11293
AB  - Mesoporous silica renders chemical and mechanical stability under biological conditions, but due to its physicochemical properties, could be potentially harmful to exposed cells. The aim of the current study was to estimate SBA-15 (concentration of 50, 100 and 250 µg/mL) impact on human peripheral blood mononuclear cells to induce cyto- and genotoxicity in vitro after 72 h treatment, as well as lipid peroxidation in serum samples ex vivo. SBA-15 mesoporous silica treatment impact on cell viability was performed by XTT assay, lipid peroxidation was estimated by determining malondialdehyde and 4-hydroxyalkenals levels and genotoxicity by micronucleus assay. SBA-15 treatments displayed genotoxic potential at the lowest concentration, while highest tested concentration led to decrease of cell viability and increase of lipid peroxidation. Chemical modification of SBA-15 material that could influence its physicochemical properties could be useful way to lower its toxicity.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry
T1  - Mesoporous silica decrease cell viability in vitro in dose dependent manner
VL  - II
SP  - 442
EP  - 444
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11293
ER  - 

@conference{
author = "Valenta-Šobot, Ana and Filipović Tričković, Jelena G. and Šaponjić, Aleksandra and Ilić, Svetlana and Grce, Ana and Katnić, Đurica and Kokunešoski, Maja",
year = "2021",
abstract = "Mesoporous silica renders chemical and mechanical stability under biological conditions, but due to its physicochemical properties, could be potentially harmful to exposed cells. The aim of the current study was to estimate SBA-15 (concentration of 50, 100 and 250 µg/mL) impact on human peripheral blood mononuclear cells to induce cyto- and genotoxicity in vitro after 72 h treatment, as well as lipid peroxidation in serum samples ex vivo. SBA-15 mesoporous silica treatment impact on cell viability was performed by XTT assay, lipid peroxidation was estimated by determining malondialdehyde and 4-hydroxyalkenals levels and genotoxicity by micronucleus assay. SBA-15 treatments displayed genotoxic potential at the lowest concentration, while highest tested concentration led to decrease of cell viability and increase of lipid peroxidation. Chemical modification of SBA-15 material that could influence its physicochemical properties could be useful way to lower its toxicity.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry",
title = "Mesoporous silica decrease cell viability in vitro in dose dependent manner",
volume = "II",
pages = "442-444",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11293"
}

Valenta-Šobot, A., Filipović Tričković, J. G., Šaponjić, A., Ilić, S., Grce, A., Katnić, Đ.,& Kokunešoski, M.. (2021). Mesoporous silica decrease cell viability in vitro in dose dependent manner. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., II, 442-444.
https://hdl.handle.net/21.15107/rcub_vinar_11293

Valenta-Šobot A, Filipović Tričković JG, Šaponjić A, Ilić S, Grce A, Katnić Đ, Kokunešoski M. Mesoporous silica decrease cell viability in vitro in dose dependent manner. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry. 2021;II:442-444.
https://hdl.handle.net/21.15107/rcub_vinar_11293 .

Valenta-Šobot, Ana, Filipović Tričković, Jelena G., Šaponjić, Aleksandra, Ilić, Svetlana, Grce, Ana, Katnić, Đurica, Kokunešoski, Maja, "Mesoporous silica decrease cell viability in vitro in dose dependent manner" in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry, II (2021):442-444,
https://hdl.handle.net/21.15107/rcub_vinar_11293 .

TY  - CONF
AU  - Valenta-Šobot, Ana
AU  - Filipović Tričković, Jelena G.
AU  - Šaponjić, Aleksandra
AU  - Grce, Ana
AU  - Kokunešoski, Maja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11310
PB  - Academy of Sciences and Arts of the Republic of Srpska
C3  - „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts
T1  - Prolonged exposure to mesoporous silica decrease cell viability in vitro
SP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11310
ER  - 

@conference{
author = "Valenta-Šobot, Ana and Filipović Tričković, Jelena G. and Šaponjić, Aleksandra and Grce, Ana and Kokunešoski, Maja",
year = "2021",
publisher = "Academy of Sciences and Arts of the Republic of Srpska",
journal = "„Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts",
title = "Prolonged exposure to mesoporous silica decrease cell viability in vitro",
pages = "53",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11310"
}

Valenta-Šobot, A., Filipović Tričković, J. G., Šaponjić, A., Grce, A.,& Kokunešoski, M.. (2021). Prolonged exposure to mesoporous silica decrease cell viability in vitro. in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts
Academy of Sciences and Arts of the Republic of Srpska., 53.
https://hdl.handle.net/21.15107/rcub_vinar_11310

Valenta-Šobot A, Filipović Tričković JG, Šaponjić A, Grce A, Kokunešoski M. Prolonged exposure to mesoporous silica decrease cell viability in vitro. in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts. 2021;:53.
https://hdl.handle.net/21.15107/rcub_vinar_11310 .

Valenta-Šobot, Ana, Filipović Tričković, Jelena G., Šaponjić, Aleksandra, Grce, Ana, Kokunešoski, Maja, "Prolonged exposure to mesoporous silica decrease cell viability in vitro" in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts (2021):53,
https://hdl.handle.net/21.15107/rcub_vinar_11310 .

TY  - JOUR
AU  - Valenta-Šobot, Ana
AU  - Savić, Jasmina
AU  - Filipović Tričković, Jelena G.
AU  - Drakulić, Dunja R.
AU  - Joksić, Gordana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10254
AB  - Root of Gentiana lutea commercially available as gentian root, a natural antidote for different types of poisons, possess antioxidative, immunomodulatory, cytoprotective and anti-inflammatory, and adverse, genotoxic and mutagenic effects. It has monoterpenes loganic acid, swertiamarin, gentiopicroside and sweroside as most abundant constituents. In this study, we assessed the toxicity of monoterpenes’ reactive molecular fragments using in silico prediction by VEGA-QSAR platform. Further, we compared the data obtained with in vitro geno- and cyto- toxicity testing of the above monoterpenes and the G. lutea root extract (GE), on human primary unstimulated and mitogen-stimulated peripheral blood mononuclear cells (PBMCs). Viability was assessed by TB and XTT tests after 48 h treatment. DNA damage was evaluated by alkaline comet assay on unstimulated cells, whereas cytokinesis-block micronucleus assay was employed on mitogen-stimulated PBMCs. Stability of compounds throughout treatment was monitored by UPLC. The observed in vitro results had highest compliance with in silico IRFMN/ISSCAN-CGX prediction model. Compounds showed high stability during experiment while treatment with single compounds reduced number of viable cells and increased DNA damage. GE treatment had toxic impact on unstimulated PBMCs but no significant genotoxic influence on mitogen-stimulated PBMCs. In summary, the mild GE effect suggests that the complexity of crude GE extract chemical composition  may attenuate the toxicity of the tested monoterpenes loganic acid, swertiamarin, gentiopicroside and sweroside.
T2  - Indian Journal of Experimental Biology
T1  - Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds
VL  - 58
IS  - 9
SP  - 609
EP  - 616
DO  - 10.56042/ijeb.v58i09.39874
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10254
ER  - 

@article{
author = "Valenta-Šobot, Ana and Savić, Jasmina and Filipović Tričković, Jelena G. and Drakulić, Dunja R. and Joksić, Gordana",
year = "2020",
abstract = "Root of Gentiana lutea commercially available as gentian root, a natural antidote for different types of poisons, possess antioxidative, immunomodulatory, cytoprotective and anti-inflammatory, and adverse, genotoxic and mutagenic effects. It has monoterpenes loganic acid, swertiamarin, gentiopicroside and sweroside as most abundant constituents. In this study, we assessed the toxicity of monoterpenes’ reactive molecular fragments using in silico prediction by VEGA-QSAR platform. Further, we compared the data obtained with in vitro geno- and cyto- toxicity testing of the above monoterpenes and the G. lutea root extract (GE), on human primary unstimulated and mitogen-stimulated peripheral blood mononuclear cells (PBMCs). Viability was assessed by TB and XTT tests after 48 h treatment. DNA damage was evaluated by alkaline comet assay on unstimulated cells, whereas cytokinesis-block micronucleus assay was employed on mitogen-stimulated PBMCs. Stability of compounds throughout treatment was monitored by UPLC. The observed in vitro results had highest compliance with in silico IRFMN/ISSCAN-CGX prediction model. Compounds showed high stability during experiment while treatment with single compounds reduced number of viable cells and increased DNA damage. GE treatment had toxic impact on unstimulated PBMCs but no significant genotoxic influence on mitogen-stimulated PBMCs. In summary, the mild GE effect suggests that the complexity of crude GE extract chemical composition  may attenuate the toxicity of the tested monoterpenes loganic acid, swertiamarin, gentiopicroside and sweroside.",
journal = "Indian Journal of Experimental Biology",
title = "Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds",
volume = "58",
number = "9",
pages = "609-616",
doi = "10.56042/ijeb.v58i09.39874",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10254"
}

Valenta-Šobot, A., Savić, J., Filipović Tričković, J. G., Drakulić, D. R.,& Joksić, G.. (2020). Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds. in Indian Journal of Experimental Biology, 58(9), 609-616.
https://doi.org/10.56042/ijeb.v58i09.39874
https://hdl.handle.net/21.15107/rcub_vinar_10254

Valenta-Šobot A, Savić J, Filipović Tričković JG, Drakulić DR, Joksić G. Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds. in Indian Journal of Experimental Biology. 2020;58(9):609-616.
doi:10.56042/ijeb.v58i09.39874
https://hdl.handle.net/21.15107/rcub_vinar_10254 .

Valenta-Šobot, Ana, Savić, Jasmina, Filipović Tričković, Jelena G., Drakulić, Dunja R., Joksić, Gordana, "Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds" in Indian Journal of Experimental Biology, 58, no. 9 (2020):609-616,
https://doi.org/10.56042/ijeb.v58i09.39874 .,
https://hdl.handle.net/21.15107/rcub_vinar_10254 .

TY  - JOUR
AU  - Valenta-Šobot, Ana
AU  - Drakulić, Dunja R.
AU  - Joksić, Gordana
AU  - Miletić Vukajlović, Jadranka
AU  - Savić, Jasmina
AU  - Potočnik, Jelena
AU  - Filipović Tričković, Jelena G.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9523
AB  - Yellow gentian (Gentiana lutea L.), a medicinal plant widely used in traditional medicine, displays multiple biological effects, ranging from beneficial to toxic. Since many promising applications have been reported so far, our aim was to evaluate its potential concentration- and time- dependent cytotoxic and genotoxic effects in vitro . To that end we exposed human peripheral blood mononuclear cells to 0.5, 1, and 2 mg/mL of yellow gentian root extract (YGRE) to determine its effects on oxidative stress parameters [pro/antioxidant balance (PAB) and lipid peroxidation], DNA damage (alkaline comet assay and chromosome aberrations), and cell viability (trypan blue exclusion test). Cell viability decreased with increasing concentrations and treatment duration. Only the lowest YGRE concentration (0.5 mg/mL) increased oxidative stress but produced minor DNA damage and cytotoxicity. At higher concentrations, redox parameters returned to near control values. The percentage of chromosome aberrations and percentage of DNA in the comet tail increased with increased YGRE concentration after 48 h and declined after 72 h of treatment. This points to the activation of DNA repair mechanism (homologous recombination), evidenced by the formation of chromosomal radial figures after 72 h of treatment with the highest YGRE concentration of 2 mg/mL. Our results suggest that YGRE, despite induction of cytotoxic and genotoxic effects, activates cell repair mechanisms that counter oxidative and DNA lesions and induce cell death in highly damaged cells. Therefore, observed protective effects of yellow gentian after longer exposure could be a result of activated repair and removal of cells with irreparable damage.
T2  - Archives of Industrial Hygiene and Toxicology
T1  - Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells
VL  - 71
IS  - 4
SP  - 320
EP  - 328
DO  - 10.2478/aiht-2020-71-3476
ER  - 

@article{
author = "Valenta-Šobot, Ana and Drakulić, Dunja R. and Joksić, Gordana and Miletić Vukajlović, Jadranka and Savić, Jasmina and Potočnik, Jelena and Filipović Tričković, Jelena G.",
year = "2020",
abstract = "Yellow gentian (Gentiana lutea L.), a medicinal plant widely used in traditional medicine, displays multiple biological effects, ranging from beneficial to toxic. Since many promising applications have been reported so far, our aim was to evaluate its potential concentration- and time- dependent cytotoxic and genotoxic effects in vitro . To that end we exposed human peripheral blood mononuclear cells to 0.5, 1, and 2 mg/mL of yellow gentian root extract (YGRE) to determine its effects on oxidative stress parameters [pro/antioxidant balance (PAB) and lipid peroxidation], DNA damage (alkaline comet assay and chromosome aberrations), and cell viability (trypan blue exclusion test). Cell viability decreased with increasing concentrations and treatment duration. Only the lowest YGRE concentration (0.5 mg/mL) increased oxidative stress but produced minor DNA damage and cytotoxicity. At higher concentrations, redox parameters returned to near control values. The percentage of chromosome aberrations and percentage of DNA in the comet tail increased with increased YGRE concentration after 48 h and declined after 72 h of treatment. This points to the activation of DNA repair mechanism (homologous recombination), evidenced by the formation of chromosomal radial figures after 72 h of treatment with the highest YGRE concentration of 2 mg/mL. Our results suggest that YGRE, despite induction of cytotoxic and genotoxic effects, activates cell repair mechanisms that counter oxidative and DNA lesions and induce cell death in highly damaged cells. Therefore, observed protective effects of yellow gentian after longer exposure could be a result of activated repair and removal of cells with irreparable damage.",
journal = "Archives of Industrial Hygiene and Toxicology",
title = "Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells",
volume = "71",
number = "4",
pages = "320-328",
doi = "10.2478/aiht-2020-71-3476"
}

Valenta-Šobot, A., Drakulić, D. R., Joksić, G., Miletić Vukajlović, J., Savić, J., Potočnik, J.,& Filipović Tričković, J. G.. (2020). Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells. in Archives of Industrial Hygiene and Toxicology, 71(4), 320-328.
https://doi.org/10.2478/aiht-2020-71-3476

Valenta-Šobot A, Drakulić DR, Joksić G, Miletić Vukajlović J, Savić J, Potočnik J, Filipović Tričković JG. Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells. in Archives of Industrial Hygiene and Toxicology. 2020;71(4):320-328.
doi:10.2478/aiht-2020-71-3476 .

Valenta-Šobot, Ana, Drakulić, Dunja R., Joksić, Gordana, Miletić Vukajlović, Jadranka, Savić, Jasmina, Potočnik, Jelena, Filipović Tričković, Jelena G., "Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells" in Archives of Industrial Hygiene and Toxicology, 71, no. 4 (2020):320-328,
https://doi.org/10.2478/aiht-2020-71-3476 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Filipović Tričković, Jelena G.
AU  - Mićić, Mileva
AU  - Joksić, Ivana
AU  - Valenta-Šobot, Ana
AU  - Demajo, Miroslav
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9729
AB  - Traditional methods in cell proliferation studies are based on immunohistochemical detection of proliferating cells in the target tissue. Since they are time consuming, optimization of novel, more efficient methods is important for large scale proliferation studies. In this study, we aimed to optimize the isolation of single epithelial rat forestomach cells for flow cytometry. As a marker of cellular proliferation we used the Ki-67 antibody to detect this nuclear protein expressed in proliferating cells. We also performed immunohistochemical detection of Ki-67 positive cells and propidium iodide staining to validate the results. 3-tert-butyl-4-hydroxyanisole was used as the positive control to ensure cellular proliferation. The results showed that isolation of epithelial cells with collagenase, trypsin and cell strainer ensures great cell viability (>95%) and the purity of the samples. Flow cytometry and immunostaining with the Ki-67 antibody indicated that 3-tert-butyl-4-hydroxyanisole treatment leads to a significant increase in proliferation. A significant positive correlation was observed between the results obtained by immunohistochemistry and flow cytometry, but the flow cytometric data had a smaller measurement error, suggesting the equal sensitivity and greater accuracy of this method. Propidium iodide staining showed that the percentage of cells in the G2+S phase of the cell cycle correlated positively with the percentage of Ki-67 positive cells assessed by flow cytometry, indicating that Ki-67 positive cells reflect an active dividing cell pool. We conclude that the isolation of forestomach epithelial cells described is a simple and reliable method for obtaining viable cells for use in flow cytometry. Compared to immunohistochemistry, flow cytometric detection of the Ki-67 antigen is equally sensitive, but much faster and provides more accurate results. © 2020, University of Zagreb, Facultty of Veterinary Medicine. All rights reserved.
T2  - Veterinarski Arhiv
T1  - Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry
VL  - 90
IS  - 5
SP  - 517
EP  - 525
DO  - 10.24099/vet.arhiv.0956
ER  - 

@article{
author = "Joksić, Gordana and Filipović Tričković, Jelena G. and Mićić, Mileva and Joksić, Ivana and Valenta-Šobot, Ana and Demajo, Miroslav",
year = "2020",
abstract = "Traditional methods in cell proliferation studies are based on immunohistochemical detection of proliferating cells in the target tissue. Since they are time consuming, optimization of novel, more efficient methods is important for large scale proliferation studies. In this study, we aimed to optimize the isolation of single epithelial rat forestomach cells for flow cytometry. As a marker of cellular proliferation we used the Ki-67 antibody to detect this nuclear protein expressed in proliferating cells. We also performed immunohistochemical detection of Ki-67 positive cells and propidium iodide staining to validate the results. 3-tert-butyl-4-hydroxyanisole was used as the positive control to ensure cellular proliferation. The results showed that isolation of epithelial cells with collagenase, trypsin and cell strainer ensures great cell viability (>95%) and the purity of the samples. Flow cytometry and immunostaining with the Ki-67 antibody indicated that 3-tert-butyl-4-hydroxyanisole treatment leads to a significant increase in proliferation. A significant positive correlation was observed between the results obtained by immunohistochemistry and flow cytometry, but the flow cytometric data had a smaller measurement error, suggesting the equal sensitivity and greater accuracy of this method. Propidium iodide staining showed that the percentage of cells in the G2+S phase of the cell cycle correlated positively with the percentage of Ki-67 positive cells assessed by flow cytometry, indicating that Ki-67 positive cells reflect an active dividing cell pool. We conclude that the isolation of forestomach epithelial cells described is a simple and reliable method for obtaining viable cells for use in flow cytometry. Compared to immunohistochemistry, flow cytometric detection of the Ki-67 antigen is equally sensitive, but much faster and provides more accurate results. © 2020, University of Zagreb, Facultty of Veterinary Medicine. All rights reserved.",
journal = "Veterinarski Arhiv",
title = "Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry",
volume = "90",
number = "5",
pages = "517-525",
doi = "10.24099/vet.arhiv.0956"
}

Joksić, G., Filipović Tričković, J. G., Mićić, M., Joksić, I., Valenta-Šobot, A.,& Demajo, M.. (2020). Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry. in Veterinarski Arhiv, 90(5), 517-525.
https://doi.org/10.24099/vet.arhiv.0956

Joksić G, Filipović Tričković JG, Mićić M, Joksić I, Valenta-Šobot A, Demajo M. Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry. in Veterinarski Arhiv. 2020;90(5):517-525.
doi:10.24099/vet.arhiv.0956 .

Joksić, Gordana, Filipović Tričković, Jelena G., Mićić, Mileva, Joksić, Ivana, Valenta-Šobot, Ana, Demajo, Miroslav, "Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry" in Veterinarski Arhiv, 90, no. 5 (2020):517-525,
https://doi.org/10.24099/vet.arhiv.0956 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Rutqvist, Lars Erik
AU  - Mićić, Mileva
AU  - Filipović Tričković, Jelena G.
AU  - Nilsson, Robert
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0273230019300601
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8080
AB  - Long term exposure to oral smokeless tobacco may induce lesions in the oral cavity characterized by a hyperplastic epithelium. The possible role of nicotine and the physical properties of oral tobacco for developing these lesions, as well as of dysplasia and neoplasia is unclear. Low nitrosamine Swedish snus as well as non-genotoxic butylated hydroxyanisole induces increased cellular proliferation in the rat forestomach epithelia. Using this model, we report here on the effects of nicotine, pH, and particle size. Snus with different properties had no impact on oxidative stress as determined by 8-oxo-7,8-dihydro-2′-deoxyguanosine, or on interleukin IL-1b. Whereas BHA boosted IL-6, probably due to the presence of nicotine. there was no significant enhancement of cell divisions with increasing particle size, although in individual samples the variations in proliferation rates increased greatly with increasing particle size. Conforming to human experience, the enhanced cell proliferation caused by snus was found to be completely reversible. A cacao bean extract had a protective action similar to that previously found for blueberries. The main cause of the observed tobacco induced cell proliferation could be mechanical irritation, possibly in combination with nicotine, whereas within the studied range, pH did not affect the rate of cell division. © 2019
T2  - Regulatory Toxicology and Pharmacology
T1  - Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus)
VL  - 104
SP  - 21
EP  - 28
DO  - 10.1016/j.yrtph.2019.02.015
ER  - 

@article{
author = "Joksić, Gordana and Rutqvist, Lars Erik and Mićić, Mileva and Filipović Tričković, Jelena G. and Nilsson, Robert",
year = "2019",
abstract = "Long term exposure to oral smokeless tobacco may induce lesions in the oral cavity characterized by a hyperplastic epithelium. The possible role of nicotine and the physical properties of oral tobacco for developing these lesions, as well as of dysplasia and neoplasia is unclear. Low nitrosamine Swedish snus as well as non-genotoxic butylated hydroxyanisole induces increased cellular proliferation in the rat forestomach epithelia. Using this model, we report here on the effects of nicotine, pH, and particle size. Snus with different properties had no impact on oxidative stress as determined by 8-oxo-7,8-dihydro-2′-deoxyguanosine, or on interleukin IL-1b. Whereas BHA boosted IL-6, probably due to the presence of nicotine. there was no significant enhancement of cell divisions with increasing particle size, although in individual samples the variations in proliferation rates increased greatly with increasing particle size. Conforming to human experience, the enhanced cell proliferation caused by snus was found to be completely reversible. A cacao bean extract had a protective action similar to that previously found for blueberries. The main cause of the observed tobacco induced cell proliferation could be mechanical irritation, possibly in combination with nicotine, whereas within the studied range, pH did not affect the rate of cell division. © 2019",
journal = "Regulatory Toxicology and Pharmacology",
title = "Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus)",
volume = "104",
pages = "21-28",
doi = "10.1016/j.yrtph.2019.02.015"
}

Joksić, G., Rutqvist, L. E., Mićić, M., Filipović Tričković, J. G.,& Nilsson, R.. (2019). Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus). in Regulatory Toxicology and Pharmacology, 104, 21-28.
https://doi.org/10.1016/j.yrtph.2019.02.015

Joksić G, Rutqvist LE, Mićić M, Filipović Tričković JG, Nilsson R. Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus). in Regulatory Toxicology and Pharmacology. 2019;104:21-28.
doi:10.1016/j.yrtph.2019.02.015 .

Joksić, Gordana, Rutqvist, Lars Erik, Mićić, Mileva, Filipović Tričković, Jelena G., Nilsson, Robert, "Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus)" in Regulatory Toxicology and Pharmacology, 104 (2019):21-28,
https://doi.org/10.1016/j.yrtph.2019.02.015 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Valenta-Šobot, Ana
AU  - Filipović Tričković, Jelena G.
AU  - Maletić, Dejan
AU  - Puač, Nevena
AU  - Malović, Gordana N.
AU  - Petrović, Zoran Lj.
AU  - Lazović, Saša
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8178
AB  - In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4-1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of 2-3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h. © 2019, Indian Academy of Sciences.
T2  - Current Science
T1  - Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation
VL  - 116
IS  - 7
SP  - 1229
EP  - 1233
DO  - 10.18520/cs/v116/i7/1229-1233
ER  - 

@article{
author = "Joksić, Gordana and Valenta-Šobot, Ana and Filipović Tričković, Jelena G. and Maletić, Dejan and Puač, Nevena and Malović, Gordana N. and Petrović, Zoran Lj. and Lazović, Saša",
year = "2019",
abstract = "In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4-1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of 2-3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h. © 2019, Indian Academy of Sciences.",
journal = "Current Science",
title = "Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation",
volume = "116",
number = "7",
pages = "1229-1233",
doi = "10.18520/cs/v116/i7/1229-1233"
}

Joksić, G., Valenta-Šobot, A., Filipović Tričković, J. G., Maletić, D., Puač, N., Malović, G. N., Petrović, Z. Lj.,& Lazović, S.. (2019). Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation. in Current Science, 116(7), 1229-1233.
https://doi.org/10.18520/cs/v116/i7/1229-1233

Joksić G, Valenta-Šobot A, Filipović Tričković JG, Maletić D, Puač N, Malović GN, Petrović ZL, Lazović S. Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation. in Current Science. 2019;116(7):1229-1233.
doi:10.18520/cs/v116/i7/1229-1233 .

Joksić, Gordana, Valenta-Šobot, Ana, Filipović Tričković, Jelena G., Maletić, Dejan, Puač, Nevena, Malović, Gordana N., Petrović, Zoran Lj., Lazović, Saša, "Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation" in Current Science, 116, no. 7 (2019):1229-1233,
https://doi.org/10.18520/cs/v116/i7/1229-1233 . .