TY  - JOUR
AU  - Milanović, Zorana
AU  - Ilić Božović, Anja
AU  - Bojić-Trbojević, Žanka
AU  - Hajduković, Ljiljana
AU  - Francuski Andrić, Jelena
AU  - Kovačević Filipović, Milica
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12998
AB  - Acute B. canis infection can lead to an acute phase reaction (APR) in dogs. The parasite invades red blood cells causinganemia through immune-mediated hemolysis and possible erythropoietic suppression. A regenerative response of theerythroid lineage during the babesiosis has not been described in extension. This research examines hematologic parametersfocusing on the absolute reticulocyte count and apolipoprotein A I (ApoA I) level on the day of admission and 14 days aftertreatment with imidocarb-dipropionate in young (n=11) and adult (n=11) dogs naturally infected with B. canis. Metabolicand inflammatory processes were characterized by analyzing protein and lipid profiles, as well as ApoA I at specified timepoints. Automated analyzers were used to determine complete blood count and biochemical parameters, while ApoA I wasassessed using radioimmunoassay. The reticulocyte count was determined using a manual method by means of supravitalstaining. Both young and adult dogs with acute B. canis infection showed non-regenerative anemia without difference.Fourteen days after successful treatment with imidocarb-dipropionate, the anemia was corrected and a high reticulocytecount was observed (p<0.05). This indicates that the erythroid regenerative response was efficient in young and adult dogs,although vital signs, leukocyte count and triglyceride concentration suggest a more intense APR in young dogs. A decreasein ApoA I in both groups 14 days after treatment (p<0.01) confirmed that this lipoprotein acts as a positive acute-phaseprotein in acute B. canis infection in dogs, but further studies are needed to connect its role in erythroid lineage regeneration.
T2  - Macedonian Veterinary Review
T1  - Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs
VL  - 47
IS  - 1
SP  - i
EP  - ix
DO  - 10.2478/macvetrev-2024-0011
ER  - 

@article{
author = "Milanović, Zorana and Ilić Božović, Anja and Bojić-Trbojević, Žanka and Hajduković, Ljiljana and Francuski Andrić, Jelena and Kovačević Filipović, Milica",
year = "2024",
abstract = "Acute B. canis infection can lead to an acute phase reaction (APR) in dogs. The parasite invades red blood cells causinganemia through immune-mediated hemolysis and possible erythropoietic suppression. A regenerative response of theerythroid lineage during the babesiosis has not been described in extension. This research examines hematologic parametersfocusing on the absolute reticulocyte count and apolipoprotein A I (ApoA I) level on the day of admission and 14 days aftertreatment with imidocarb-dipropionate in young (n=11) and adult (n=11) dogs naturally infected with B. canis. Metabolicand inflammatory processes were characterized by analyzing protein and lipid profiles, as well as ApoA I at specified timepoints. Automated analyzers were used to determine complete blood count and biochemical parameters, while ApoA I wasassessed using radioimmunoassay. The reticulocyte count was determined using a manual method by means of supravitalstaining. Both young and adult dogs with acute B. canis infection showed non-regenerative anemia without difference.Fourteen days after successful treatment with imidocarb-dipropionate, the anemia was corrected and a high reticulocytecount was observed (p<0.05). This indicates that the erythroid regenerative response was efficient in young and adult dogs,although vital signs, leukocyte count and triglyceride concentration suggest a more intense APR in young dogs. A decreasein ApoA I in both groups 14 days after treatment (p<0.01) confirmed that this lipoprotein acts as a positive acute-phaseprotein in acute B. canis infection in dogs, but further studies are needed to connect its role in erythroid lineage regeneration.",
journal = "Macedonian Veterinary Review",
title = "Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs",
volume = "47",
number = "1",
pages = "i-ix",
doi = "10.2478/macvetrev-2024-0011"
}

Milanović, Z., Ilić Božović, A., Bojić-Trbojević, Ž., Hajduković, L., Francuski Andrić, J.,& Kovačević Filipović, M.. (2024). Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs. in Macedonian Veterinary Review, 47(1), i-ix.
https://doi.org/10.2478/macvetrev-2024-0011

Milanović Z, Ilić Božović A, Bojić-Trbojević Ž, Hajduković L, Francuski Andrić J, Kovačević Filipović M. Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs. in Macedonian Veterinary Review. 2024;47(1):i-ix.
doi:10.2478/macvetrev-2024-0011 .

Milanović, Zorana, Ilić Božović, Anja, Bojić-Trbojević, Žanka, Hajduković, Ljiljana, Francuski Andrić, Jelena, Kovačević Filipović, Milica, "Reticulocyte response in paired blood samples of Babesia canis infected young and adult dogs" in Macedonian Veterinary Review, 47, no. 1 (2024):i-ix,
https://doi.org/10.2478/macvetrev-2024-0011 . .

TY  - CONF
AU  - Knežević, Sanja
AU  - Kosanović, Dejana
AU  - Živković, Irena
AU  - Ilić, Vesna
AU  - Dragačević, Luka
AU  - Hajduković, Ljiljana
AU  - Minić, Rajna
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11575
AB  - Streptococcus pneumoniae, the pneumococcus is an important human pathogen. It possesses a capsule which has been thoroughly studied, since it is an important factor in virulence. In this work S. pneumoniae ATCC 6301, belonging to Serogroup 1, was used as a model organism to determine the levels of different IgG isotypes specific to this bacterium in different sex and age groups. For this purpose an in‐house ELISA was used. Although IgG2 is a major antibacterial antibody subclass, acting against polysaccharide antigens, the level of pneumococcus‐specific IgG2 did not differ from the level of IgG2 specific to several tested lactic acid bacteria (LAB). On the other hand pneumococcus specific IgG1 level was significantly higher to the level of LAB specific IgG1. This is most likely a consequence of previous encounters with pathogenic pneumococcal microorganism and a distinguishing characteristic of this encounter. By analyzing different sex and age groups we found significantly lower anti‐pneumococcus IgG1 levels in men, implying lower protection against invasion with pneumococcus, which possibly translates to other encapsulated bacteria. The Covid‐19 pandemic has identified males as being at increased risk of hospital admission and death and lower levels of anti‐pneumococcus antibodies might be a contributing factor. Hence, we advocate the immunization with conjugate pneumococcal vaccines in male individuals, especially over the age of 50.
PB  - Wiley
C3  - European Journal of Immunology, Virtual ECI 2021 : Abstract Book
T1  - Report on lower anti-pneumococcus IgG1 levels in men, especially over the age of fifty
VL  - 51
IS  - Supplement S1
SP  - 337
EP  - 337
DO  - 10.1002/eji.202170200
ER  - 

@conference{
author = "Knežević, Sanja and Kosanović, Dejana and Živković, Irena and Ilić, Vesna and Dragačević, Luka and Hajduković, Ljiljana and Minić, Rajna",
year = "2021",
abstract = "Streptococcus pneumoniae, the pneumococcus is an important human pathogen. It possesses a capsule which has been thoroughly studied, since it is an important factor in virulence. In this work S. pneumoniae ATCC 6301, belonging to Serogroup 1, was used as a model organism to determine the levels of different IgG isotypes specific to this bacterium in different sex and age groups. For this purpose an in‐house ELISA was used. Although IgG2 is a major antibacterial antibody subclass, acting against polysaccharide antigens, the level of pneumococcus‐specific IgG2 did not differ from the level of IgG2 specific to several tested lactic acid bacteria (LAB). On the other hand pneumococcus specific IgG1 level was significantly higher to the level of LAB specific IgG1. This is most likely a consequence of previous encounters with pathogenic pneumococcal microorganism and a distinguishing characteristic of this encounter. By analyzing different sex and age groups we found significantly lower anti‐pneumococcus IgG1 levels in men, implying lower protection against invasion with pneumococcus, which possibly translates to other encapsulated bacteria. The Covid‐19 pandemic has identified males as being at increased risk of hospital admission and death and lower levels of anti‐pneumococcus antibodies might be a contributing factor. Hence, we advocate the immunization with conjugate pneumococcal vaccines in male individuals, especially over the age of 50.",
publisher = "Wiley",
journal = "European Journal of Immunology, Virtual ECI 2021 : Abstract Book",
title = "Report on lower anti-pneumococcus IgG1 levels in men, especially over the age of fifty",
volume = "51",
number = "Supplement S1",
pages = "337-337",
doi = "10.1002/eji.202170200"
}

Knežević, S., Kosanović, D., Živković, I., Ilić, V., Dragačević, L., Hajduković, L.,& Minić, R.. (2021). Report on lower anti-pneumococcus IgG1 levels in men, especially over the age of fifty. in European Journal of Immunology, Virtual ECI 2021 : Abstract Book
Wiley., 51(Supplement S1), 337-337.
https://doi.org/10.1002/eji.202170200

Knežević S, Kosanović D, Živković I, Ilić V, Dragačević L, Hajduković L, Minić R. Report on lower anti-pneumococcus IgG1 levels in men, especially over the age of fifty. in European Journal of Immunology, Virtual ECI 2021 : Abstract Book. 2021;51(Supplement S1):337-337.
doi:10.1002/eji.202170200 .

Knežević, Sanja, Kosanović, Dejana, Živković, Irena, Ilić, Vesna, Dragačević, Luka, Hajduković, Ljiljana, Minić, Rajna, "Report on lower anti-pneumococcus IgG1 levels in men, especially over the age of fifty" in European Journal of Immunology, Virtual ECI 2021 : Abstract Book, 51, no. Supplement S1 (2021):337-337,
https://doi.org/10.1002/eji.202170200 . .