TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Vasić, Vesna M.
AU  - Bondžić, Aleksandra M.
AU  - Uscumlic, Gordana S.
AU  - Petrovic, Slobodan D.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5599
AB  - Organophosphorus insecticides have been the most applied group of insectcides for the last two decades. Their main toxic effects are related to irreversible inactivation of acetylcholinesterase (AChE). Actually, they covalently bind to serine OH group in the enzyme active site forming phosphorylated enzyme that cannot hydrolyze acetylcholine. Organophosphorus insecticides in the environment undergo the natural degradation pathway including mainly homogeneous and heterogeneous hydrolysis (especially at high pH) generating non-inhibiting products. Additionally, thio organophosphates are easily oxidized by naturally present oxidants and UV light, forming more toxic and stable oxons. Thus, oxidative degradation procedures, generally referred as advanced oxidation processes (AOP), have been applied for their efficient removal from contaminated waters. The most applied bioassays to monitor the organophosphate toxicity, i.e., the detoxification degree during AOP are Vibrio fischeri and AChE bioassays. Vibrio fischeri toxicity test exploits bioluminescence as the measure of luciferase activity of this marine bacterium, whereas AChE bioassay is based on AChE activity inhibition. Both bioanalytical techniques are rapid (several minutes), simple, sensitive and reproducible. Vibrio fischeri test seems to be a versatile indicator of toxic compounds generated in AOP for organophosphorus insecticides degradation. However, detection of neurotoxic AChE inhibitors, which can be formed in AOP of some organophosphates, requires AChE bioassays. Therefore, AChE toxicity test is more appropriate for monitoring the degradation processes of thio organophosphates, because more toxic oxo organophosphates might be formed and overlooked by Vibrio fischeri bioluminescence inhibition. In addition, during organophosphates removal by AOP, compounds with strong genotoxic potential may be formed, which cannot be detected by standard toxicity tests. For this reason, determination of incidence of micronuclei and cell proliferation index in cultivated human lymphocytes and fibroblasts is suitable for evaluation of organophosphorus insecticides and their break down products inducing cytogenetic damage.
T2  - Hemijska industrija
T1  - Organophosphorus Insecticides: Toxic Effects and Bioanalytical Tests for Evaluating Toxicity During Degradation Processes
VL  - 67
IS  - 2
SP  - 217
EP  - 230
DO  - 10.2298/HEMIND120323060C
ER  - 

@article{
author = "Čolović, Mirjana B. and Krstić, Danijela Z. and Vasić, Vesna M. and Bondžić, Aleksandra M. and Uscumlic, Gordana S. and Petrovic, Slobodan D.",
year = "2013",
abstract = "Organophosphorus insecticides have been the most applied group of insectcides for the last two decades. Their main toxic effects are related to irreversible inactivation of acetylcholinesterase (AChE). Actually, they covalently bind to serine OH group in the enzyme active site forming phosphorylated enzyme that cannot hydrolyze acetylcholine. Organophosphorus insecticides in the environment undergo the natural degradation pathway including mainly homogeneous and heterogeneous hydrolysis (especially at high pH) generating non-inhibiting products. Additionally, thio organophosphates are easily oxidized by naturally present oxidants and UV light, forming more toxic and stable oxons. Thus, oxidative degradation procedures, generally referred as advanced oxidation processes (AOP), have been applied for their efficient removal from contaminated waters. The most applied bioassays to monitor the organophosphate toxicity, i.e., the detoxification degree during AOP are Vibrio fischeri and AChE bioassays. Vibrio fischeri toxicity test exploits bioluminescence as the measure of luciferase activity of this marine bacterium, whereas AChE bioassay is based on AChE activity inhibition. Both bioanalytical techniques are rapid (several minutes), simple, sensitive and reproducible. Vibrio fischeri test seems to be a versatile indicator of toxic compounds generated in AOP for organophosphorus insecticides degradation. However, detection of neurotoxic AChE inhibitors, which can be formed in AOP of some organophosphates, requires AChE bioassays. Therefore, AChE toxicity test is more appropriate for monitoring the degradation processes of thio organophosphates, because more toxic oxo organophosphates might be formed and overlooked by Vibrio fischeri bioluminescence inhibition. In addition, during organophosphates removal by AOP, compounds with strong genotoxic potential may be formed, which cannot be detected by standard toxicity tests. For this reason, determination of incidence of micronuclei and cell proliferation index in cultivated human lymphocytes and fibroblasts is suitable for evaluation of organophosphorus insecticides and their break down products inducing cytogenetic damage.",
journal = "Hemijska industrija",
title = "Organophosphorus Insecticides: Toxic Effects and Bioanalytical Tests for Evaluating Toxicity During Degradation Processes",
volume = "67",
number = "2",
pages = "217-230",
doi = "10.2298/HEMIND120323060C"
}

Čolović, M. B., Krstić, D. Z., Vasić, V. M., Bondžić, A. M., Uscumlic, G. S.,& Petrovic, S. D.. (2013). Organophosphorus Insecticides: Toxic Effects and Bioanalytical Tests for Evaluating Toxicity During Degradation Processes. in Hemijska industrija, 67(2), 217-230.
https://doi.org/10.2298/HEMIND120323060C

Čolović MB, Krstić DZ, Vasić VM, Bondžić AM, Uscumlic GS, Petrovic SD. Organophosphorus Insecticides: Toxic Effects and Bioanalytical Tests for Evaluating Toxicity During Degradation Processes. in Hemijska industrija. 2013;67(2):217-230.
doi:10.2298/HEMIND120323060C .

Čolović, Mirjana B., Krstić, Danijela Z., Vasić, Vesna M., Bondžić, Aleksandra M., Uscumlic, Gordana S., Petrovic, Slobodan D., "Organophosphorus Insecticides: Toxic Effects and Bioanalytical Tests for Evaluating Toxicity During Degradation Processes" in Hemijska industrija, 67, no. 2 (2013):217-230,
https://doi.org/10.2298/HEMIND120323060C . .

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Uscumlic, Gordana S.
AU  - Vasić, Vesna M.
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4275
AB  - In vitro inhibition of electric eel acetylcholinesterase (AChE) by single and simultaneous exposure to organophosphorus insecticides diazinon and chlorpyrifos, and their transformation products, formed due to photoinduced degradation, was investigated. Increasing concentrations of diazinon, chlorpyrifos and their oxidation products, diazoxon and chlorpyrifos-oxon, inhibited AChE in a concentration-dependent manner. IC50 (20 min) values, obtained from the inhibition curves, were (in mol/l): (5.1 +/- 0.3) x 10(-8) (4.3 +/- 0.2) x 10(-6) and (3.0 +/- 0.1) x 10(-8) for diazoxon, chlorpyrifos and chlorpyrifos-oxon, respectively, while maximal diazinon concentration was lower than its IC50 (20 min). Calculated K-I values, in mol/l, of 7.9 x 10(-7), 9.6 x 10(-6) and 4.3 x 10(-7) were obtained for diazoxon, chlorpyrifos and chlorpyrifos-oxon, respectively. However, 2-isopropyl-4-methyl-6-pyrimidinol (IMP) and 3,5,6-trichloro-2-pyridinol, diazinon and chlorpyrifos hydrolysis products, did not noticeably affect the enzyme activity at all investigated concentrations. Additive inhibition effect was achieved for lower concentrations of the inhibitors (diazinon/diazoxon LT = 1 x 10(-4)/1 x 10(-8) mol/l i.e., chlorpyrifos/chlorpyrifos-oxon LT = 2 x 10(-6)/3 x 10(-8) mol/l), while an antagonistic effect was obtained for all higher concentrations of the organophosphates. Inhibitory power of 1 x 10(-4) mol/l diazinon irradiated samples can be attributed mostly to the formation of diazoxon, while the presence of non-inhibiting photodegradation product IMP did not affect diazinon and diazoxon inhibitory efficiencies. (C) 2011 Elsevier Inc. All rights reserved.
T2  - Pesticide Biochemistry and Physiology
T1  - Single and simultaneous exposure of acetylcholinesterase to diazinon, chlorpyrifos and their photodegradation products
VL  - 100
IS  - 1
SP  - 16
EP  - 22
DO  - 10.1016/j.pestbp.2011.01.010
ER  - 

@article{
author = "Čolović, Mirjana B. and Krstić, Danijela Z. and Uscumlic, Gordana S. and Vasić, Vesna M.",
year = "2011",
abstract = "In vitro inhibition of electric eel acetylcholinesterase (AChE) by single and simultaneous exposure to organophosphorus insecticides diazinon and chlorpyrifos, and their transformation products, formed due to photoinduced degradation, was investigated. Increasing concentrations of diazinon, chlorpyrifos and their oxidation products, diazoxon and chlorpyrifos-oxon, inhibited AChE in a concentration-dependent manner. IC50 (20 min) values, obtained from the inhibition curves, were (in mol/l): (5.1 +/- 0.3) x 10(-8) (4.3 +/- 0.2) x 10(-6) and (3.0 +/- 0.1) x 10(-8) for diazoxon, chlorpyrifos and chlorpyrifos-oxon, respectively, while maximal diazinon concentration was lower than its IC50 (20 min). Calculated K-I values, in mol/l, of 7.9 x 10(-7), 9.6 x 10(-6) and 4.3 x 10(-7) were obtained for diazoxon, chlorpyrifos and chlorpyrifos-oxon, respectively. However, 2-isopropyl-4-methyl-6-pyrimidinol (IMP) and 3,5,6-trichloro-2-pyridinol, diazinon and chlorpyrifos hydrolysis products, did not noticeably affect the enzyme activity at all investigated concentrations. Additive inhibition effect was achieved for lower concentrations of the inhibitors (diazinon/diazoxon LT = 1 x 10(-4)/1 x 10(-8) mol/l i.e., chlorpyrifos/chlorpyrifos-oxon LT = 2 x 10(-6)/3 x 10(-8) mol/l), while an antagonistic effect was obtained for all higher concentrations of the organophosphates. Inhibitory power of 1 x 10(-4) mol/l diazinon irradiated samples can be attributed mostly to the formation of diazoxon, while the presence of non-inhibiting photodegradation product IMP did not affect diazinon and diazoxon inhibitory efficiencies. (C) 2011 Elsevier Inc. All rights reserved.",
journal = "Pesticide Biochemistry and Physiology",
title = "Single and simultaneous exposure of acetylcholinesterase to diazinon, chlorpyrifos and their photodegradation products",
volume = "100",
number = "1",
pages = "16-22",
doi = "10.1016/j.pestbp.2011.01.010"
}

Čolović, M. B., Krstić, D. Z., Uscumlic, G. S.,& Vasić, V. M.. (2011). Single and simultaneous exposure of acetylcholinesterase to diazinon, chlorpyrifos and their photodegradation products. in Pesticide Biochemistry and Physiology, 100(1), 16-22.
https://doi.org/10.1016/j.pestbp.2011.01.010

Čolović MB, Krstić DZ, Uscumlic GS, Vasić VM. Single and simultaneous exposure of acetylcholinesterase to diazinon, chlorpyrifos and their photodegradation products. in Pesticide Biochemistry and Physiology. 2011;100(1):16-22.
doi:10.1016/j.pestbp.2011.01.010 .

Čolović, Mirjana B., Krstić, Danijela Z., Uscumlic, Gordana S., Vasić, Vesna M., "Single and simultaneous exposure of acetylcholinesterase to diazinon, chlorpyrifos and their photodegradation products" in Pesticide Biochemistry and Physiology, 100, no. 1 (2011):16-22,
https://doi.org/10.1016/j.pestbp.2011.01.010 . .