TY  - JOUR
AU  - Mićović, Žarko
AU  - Kostić, Sanja
AU  - Mutavdžin, Slavica
AU  - Andrejević, Aleksa
AU  - Stamenković, Aleksandra
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
AU  - Đurić, Marko
AU  - Hrnčić, Dragan
AU  - Živković, Vladimir
AU  - Jakovljević, Vladimir
AU  - Đurić, Dragan
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12970
AB  - Background/Aim. Chronically induced hypermethioninemia leads to hyperhomocysteinemia which causes oxidative stress, atherogenesis, neurodegeneration and cancer. However, little is known about the acute and subchronic effects of DL-methionine (Met). The aim of study was to assess the effects of acutely and subchronically applied Met on oxidative stress parameters in rat plasma [enzymes: catalase (CAT), glutathione peroxidise (GPx), superoxide dismutase (SOD) and index of lipid peroxidation, malondialdehyde (MDA)], and acetylcholinesterase (AChE) activity in rat cardiac tissue. Methods. The enzymes activities, as well as MDA concentration were evaluated following acute (n = 8) and subchronic (n = 10) application of Met [i.p. 0.8 mmoL/kg body weight (b.w.) in a single dose in the acute overload or daily during three weeks in the subchronic overload]. The same was done in the control groups following application of physiological solution [i.p. 1 mL 0.9% NaCl (n = 8) in the acute overload and 0.1–0.2 mL 0.9% NaCl, daily during three weeks (n =10) in the subchronic overload]. Tested parameters were evaluated 60 minutes after application in acute experiments and after three weeks of treatment in subchronic experiments. Results. There were no difference in homocysteine values between the groups treated with Met for three weeks and the control group. Met administration significantly increased the activity of CAT and GPx after 1 h compared to the control group (p = 0.008 for both enzymes), whereas the activity of SOD and MDA concentrations were unchanged. Subchronically applied Met did not affect activity of antioxidant enzymes and MDA level. AChE activity did not show any change in rat cardiac tissue after 1 h, but it was significantly decreased after the subchronic treatment (p = 0.041). Conclusion. Results of present research indicate that Met differently affects estimated parameters during acute and subchronic application. In the acute treatment Met mobilizes the most part of antioxidant enzymes while during the subchronic treatment these changes seems to be lost. On the contrary, the acute Met overload was not sufficient to influence on the AChE activity, while longer duration of Met loading diminished function of the enzyme. These findings point out that methionine can interfere with antioxidant defense system and cholinergic control of the heart function.
T2  - Vojnosanitetski pregled
T1  - The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue
VL  - 77
IS  - 2
SP  - 165
EP  - 173
DO  - 10.2298/VSP171213055M
ER  - 

@article{
author = "Mićović, Žarko and Kostić, Sanja and Mutavdžin, Slavica and Andrejević, Aleksa and Stamenković, Aleksandra and Čolović, Mirjana and Krstić, Danijela and Đurić, Marko and Hrnčić, Dragan and Živković, Vladimir and Jakovljević, Vladimir and Đurić, Dragan",
year = "2020",
abstract = "Background/Aim. Chronically induced hypermethioninemia leads to hyperhomocysteinemia which causes oxidative stress, atherogenesis, neurodegeneration and cancer. However, little is known about the acute and subchronic effects of DL-methionine (Met). The aim of study was to assess the effects of acutely and subchronically applied Met on oxidative stress parameters in rat plasma [enzymes: catalase (CAT), glutathione peroxidise (GPx), superoxide dismutase (SOD) and index of lipid peroxidation, malondialdehyde (MDA)], and acetylcholinesterase (AChE) activity in rat cardiac tissue. Methods. The enzymes activities, as well as MDA concentration were evaluated following acute (n = 8) and subchronic (n = 10) application of Met [i.p. 0.8 mmoL/kg body weight (b.w.) in a single dose in the acute overload or daily during three weeks in the subchronic overload]. The same was done in the control groups following application of physiological solution [i.p. 1 mL 0.9% NaCl (n = 8) in the acute overload and 0.1–0.2 mL 0.9% NaCl, daily during three weeks (n =10) in the subchronic overload]. Tested parameters were evaluated 60 minutes after application in acute experiments and after three weeks of treatment in subchronic experiments. Results. There were no difference in homocysteine values between the groups treated with Met for three weeks and the control group. Met administration significantly increased the activity of CAT and GPx after 1 h compared to the control group (p = 0.008 for both enzymes), whereas the activity of SOD and MDA concentrations were unchanged. Subchronically applied Met did not affect activity of antioxidant enzymes and MDA level. AChE activity did not show any change in rat cardiac tissue after 1 h, but it was significantly decreased after the subchronic treatment (p = 0.041). Conclusion. Results of present research indicate that Met differently affects estimated parameters during acute and subchronic application. In the acute treatment Met mobilizes the most part of antioxidant enzymes while during the subchronic treatment these changes seems to be lost. On the contrary, the acute Met overload was not sufficient to influence on the AChE activity, while longer duration of Met loading diminished function of the enzyme. These findings point out that methionine can interfere with antioxidant defense system and cholinergic control of the heart function.",
journal = "Vojnosanitetski pregled",
title = "The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue",
volume = "77",
number = "2",
pages = "165-173",
doi = "10.2298/VSP171213055M"
}

Mićović, Ž., Kostić, S., Mutavdžin, S., Andrejević, A., Stamenković, A., Čolović, M., Krstić, D., Đurić, M., Hrnčić, D., Živković, V., Jakovljević, V.,& Đurić, D.. (2020). The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue. in Vojnosanitetski pregled, 77(2), 165-173.
https://doi.org/10.2298/VSP171213055M

Mićović Ž, Kostić S, Mutavdžin S, Andrejević A, Stamenković A, Čolović M, Krstić D, Đurić M, Hrnčić D, Živković V, Jakovljević V, Đurić D. The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue. in Vojnosanitetski pregled. 2020;77(2):165-173.
doi:10.2298/VSP171213055M .

Mićović, Žarko, Kostić, Sanja, Mutavdžin, Slavica, Andrejević, Aleksa, Stamenković, Aleksandra, Čolović, Mirjana, Krstić, Danijela, Đurić, Marko, Hrnčić, Dragan, Živković, Vladimir, Jakovljević, Vladimir, Đurić, Dragan, "The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue" in Vojnosanitetski pregled, 77, no. 2 (2020):165-173,
https://doi.org/10.2298/VSP171213055M . .

TY  - JOUR
AU  - Đurić, Marko
AU  - Mutavdžin, Slavica
AU  - Lončar-Stojiljković, Dragana
AU  - Kostić, Sanja
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
AU  - Živković, Vladimir
AU  - Jakovljević, Vladimir
AU  - Đurić, Dragan
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12972
AB  - Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group – saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ sacrifice. AChE activity was measured in the rats’ cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+LNAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters.
T2  - Scripta Medica
T1  - The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity
VL  - 50
IS  - 3
SP  - 112
EP  - 116
DO  - 10.5937/scriptamed50-22658
ER  - 

@article{
author = "Đurić, Marko and Mutavdžin, Slavica and Lončar-Stojiljković, Dragana and Kostić, Sanja and Čolović, Mirjana and Krstić, Danijela and Živković, Vladimir and Jakovljević, Vladimir and Đurić, Dragan",
year = "2019",
abstract = "Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group – saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ sacrifice. AChE activity was measured in the rats’ cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+LNAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters.",
journal = "Scripta Medica",
title = "The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity",
volume = "50",
number = "3",
pages = "112-116",
doi = "10.5937/scriptamed50-22658"
}

Đurić, M., Mutavdžin, S., Lončar-Stojiljković, D., Kostić, S., Čolović, M., Krstić, D., Živković, V., Jakovljević, V.,& Đurić, D.. (2019). The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity. in Scripta Medica, 50(3), 112-116.
https://doi.org/10.5937/scriptamed50-22658

Đurić M, Mutavdžin S, Lončar-Stojiljković D, Kostić S, Čolović M, Krstić D, Živković V, Jakovljević V, Đurić D. The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity. in Scripta Medica. 2019;50(3):112-116.
doi:10.5937/scriptamed50-22658 .

Đurić, Marko, Mutavdžin, Slavica, Lončar-Stojiljković, Dragana, Kostić, Sanja, Čolović, Mirjana, Krstić, Danijela, Živković, Vladimir, Jakovljević, Vladimir, Đurić, Dragan, "The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity" in Scripta Medica, 50, no. 3 (2019):112-116,
https://doi.org/10.5937/scriptamed50-22658 . .

TY  - JOUR
AU  - Kornjača, Duško
AU  - Živković, Vladimir I.
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Marko
AU  - Stanković, Sanja
AU  - Mutavdžin, Slavica
AU  - Jakovljević, Vladimir Lj.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641700041K
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7714
AB  - The aim of this study was to assess the effects of DL-homocysteine (DL-Hcy) and DL-homocysteine thiolactone (DL-Hcy TLHC) on selected serum biochemical parameters, markers of oxidative stress and the activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) in the plasma, as well as on acetylcholinesterase (AChE) activity in the cardiac tissue homogenate in the rat. Male Wistar rats were divided into three groups as follows: control group (1 mL 0.9% NaCl, intraperitoneal (i.p.) injection), DL-Hcy group (8 mmol/kg body mass (b.m.), i.p.) or DL-Hcy TLHC group (8 mmol/kg b.m., i.p.). One hour after administration, the rats were euthanized, whole blood was collected for biochemical analysis, and the heart was excised. Following the i.p. administration of DL-Hcy and DL-Hcy TLHC, the activities of antioxidant enzymes were mostly significantly increased, while plasma malondialdehyde (MDA) was decreased. Administration of DL-Hcy and DL-Hcy TLHC significantly inhibited AChE activity in rat cardiac tissue. Our findings suggest that DL-Hcy and DL-Hcy TLHC exerted prooxidant effects; however, the decrease in MDA points to an inverse response to the increase in antioxidant enzyme activities. While both substances inhibited AChE activity in rat cardiac tissue, DL-Hcy TLHC induced stronger effects than DL-Hcy.
T2  - Archives of Biological Sciences
T1  - The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat
VL  - 70
IS  - 2
SP  - 241
EP  - 248
DO  - 10.2298/ABS170731041K
ER  - 

@article{
author = "Kornjača, Duško and Živković, Vladimir I. and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Marko and Stanković, Sanja and Mutavdžin, Slavica and Jakovljević, Vladimir Lj. and Đurić, Dragan M.",
year = "2018",
abstract = "The aim of this study was to assess the effects of DL-homocysteine (DL-Hcy) and DL-homocysteine thiolactone (DL-Hcy TLHC) on selected serum biochemical parameters, markers of oxidative stress and the activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) in the plasma, as well as on acetylcholinesterase (AChE) activity in the cardiac tissue homogenate in the rat. Male Wistar rats were divided into three groups as follows: control group (1 mL 0.9% NaCl, intraperitoneal (i.p.) injection), DL-Hcy group (8 mmol/kg body mass (b.m.), i.p.) or DL-Hcy TLHC group (8 mmol/kg b.m., i.p.). One hour after administration, the rats were euthanized, whole blood was collected for biochemical analysis, and the heart was excised. Following the i.p. administration of DL-Hcy and DL-Hcy TLHC, the activities of antioxidant enzymes were mostly significantly increased, while plasma malondialdehyde (MDA) was decreased. Administration of DL-Hcy and DL-Hcy TLHC significantly inhibited AChE activity in rat cardiac tissue. Our findings suggest that DL-Hcy and DL-Hcy TLHC exerted prooxidant effects; however, the decrease in MDA points to an inverse response to the increase in antioxidant enzyme activities. While both substances inhibited AChE activity in rat cardiac tissue, DL-Hcy TLHC induced stronger effects than DL-Hcy.",
journal = "Archives of Biological Sciences",
title = "The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat",
volume = "70",
number = "2",
pages = "241-248",
doi = "10.2298/ABS170731041K"
}

Kornjača, D., Živković, V. I., Krstić, D. Z., Čolović, M. B., Đurić, M., Stanković, S., Mutavdžin, S., Jakovljević, V. Lj.,& Đurić, D. M.. (2018). The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat. in Archives of Biological Sciences, 70(2), 241-248.
https://doi.org/10.2298/ABS170731041K

Kornjača D, Živković VI, Krstić DZ, Čolović MB, Đurić M, Stanković S, Mutavdžin S, Jakovljević VL, Đurić DM. The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat. in Archives of Biological Sciences. 2018;70(2):241-248.
doi:10.2298/ABS170731041K .

Kornjača, Duško, Živković, Vladimir I., Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Marko, Stanković, Sanja, Mutavdžin, Slavica, Jakovljević, Vladimir Lj., Đurić, Dragan M., "The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat" in Archives of Biological Sciences, 70, no. 2 (2018):241-248,
https://doi.org/10.2298/ABS170731041K . .

TY  - JOUR
AU  - Milutinović, Milan M.
AU  - Čanović, Petar P.
AU  - Stevanović, Dragana D.
AU  - Masnikosa, Romana
AU  - Vraneš, Milan
AU  - Tot, Aleksandar
AU  - Zarić, Milan M.
AU  - Marković-Simović, Bojana
AU  - Misirkić-Marjanović, Maja
AU  - Vučićević, Ljubica
AU  - Savić, Maja
AU  - Jakovljević, Vladimir Lj.
AU  - Trajković, Vladimir S.
AU  - Volarević, Vladislav
AU  - Kanjevac, Tatjana
AU  - Rilak Simović, Ana
PY  - 2018
UR  - http://pubs.acs.org/doi/10.1021/acs.organomet.8b00604
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7967
AB  - The two new heterometallic Ru(II)-tpy/ferrocene complexes [Ru(tpy)Cl2(mtefc)] (1) and [Ru(tpy)Cl2(mtpfc)] (2) (where tpy = 2,2′:6′,2′′-terpyridine, mtefc = (2-(methylthio)ethyl)ferrocene, and mtpfc = (3-(methylthio)propyl)ferrocene) have been synthesized and then characterized through elemental analysis, followed by various spectroscopic (IR, UV-vis, 1D and 2D NMR) and mass spectrometric techniques (MALDI TOF and ESI Q-TOF MS). UV-vis and fluorescence spectroscopy and viscometry were employed to study the interactions of the complexes 1 and 2 with calf thymus DNA. Both 1 and 2 expelled ethidium bromide (EB) from the EB/DNA complex (Ksv = (1.5-1.8) × 104 M-1), which suggested that the complexes intercalated into the double helix of DNA. Both complexes strongly quenched the fluorescence of tryptophan residues in serum albumin through both static and dynamic quenching. Molecular docking confirmed the intercalative mode of complex interaction with DNA. The docking results implied that 1 and 2 interacted with hydrophobic residues of albumin, particularly with those lying in the proximity of Tyr 160. We here demonstrate the high cytotoxic potential of complexes 1 and 2 against the breast cancer cells that originated either from humans (MDA-MB-231) or from mice (4T1), with apoptosis being the main mechanism of complex-induced cell death. It is worth noting that both complexes promoted activation of innate and acquired antitumor immunity, which contributed to the reduced growth and progression of mammary carcinoma in vivo. Copyright © 2018 American Chemical Society.
T2  - Organometallics
T1  - Newly Synthesized Heteronuclear Ruthenium(II)/Ferrocene Complexes Suppress the Growth of Mammary Carcinoma in 4T1-Treated BALB/c Mice by Promoting Activation of Antitumor Immunity
VL  - 37
IS  - 22
SP  - 4250
EP  - 4266
DO  - 10.1021/acs.organomet.8b00604
ER  - 

@article{
author = "Milutinović, Milan M. and Čanović, Petar P. and Stevanović, Dragana D. and Masnikosa, Romana and Vraneš, Milan and Tot, Aleksandar and Zarić, Milan M. and Marković-Simović, Bojana and Misirkić-Marjanović, Maja and Vučićević, Ljubica and Savić, Maja and Jakovljević, Vladimir Lj. and Trajković, Vladimir S. and Volarević, Vladislav and Kanjevac, Tatjana and Rilak Simović, Ana",
year = "2018",
abstract = "The two new heterometallic Ru(II)-tpy/ferrocene complexes [Ru(tpy)Cl2(mtefc)] (1) and [Ru(tpy)Cl2(mtpfc)] (2) (where tpy = 2,2′:6′,2′′-terpyridine, mtefc = (2-(methylthio)ethyl)ferrocene, and mtpfc = (3-(methylthio)propyl)ferrocene) have been synthesized and then characterized through elemental analysis, followed by various spectroscopic (IR, UV-vis, 1D and 2D NMR) and mass spectrometric techniques (MALDI TOF and ESI Q-TOF MS). UV-vis and fluorescence spectroscopy and viscometry were employed to study the interactions of the complexes 1 and 2 with calf thymus DNA. Both 1 and 2 expelled ethidium bromide (EB) from the EB/DNA complex (Ksv = (1.5-1.8) × 104 M-1), which suggested that the complexes intercalated into the double helix of DNA. Both complexes strongly quenched the fluorescence of tryptophan residues in serum albumin through both static and dynamic quenching. Molecular docking confirmed the intercalative mode of complex interaction with DNA. The docking results implied that 1 and 2 interacted with hydrophobic residues of albumin, particularly with those lying in the proximity of Tyr 160. We here demonstrate the high cytotoxic potential of complexes 1 and 2 against the breast cancer cells that originated either from humans (MDA-MB-231) or from mice (4T1), with apoptosis being the main mechanism of complex-induced cell death. It is worth noting that both complexes promoted activation of innate and acquired antitumor immunity, which contributed to the reduced growth and progression of mammary carcinoma in vivo. Copyright © 2018 American Chemical Society.",
journal = "Organometallics",
title = "Newly Synthesized Heteronuclear Ruthenium(II)/Ferrocene Complexes Suppress the Growth of Mammary Carcinoma in 4T1-Treated BALB/c Mice by Promoting Activation of Antitumor Immunity",
volume = "37",
number = "22",
pages = "4250-4266",
doi = "10.1021/acs.organomet.8b00604"
}

Milutinović, M. M., Čanović, P. P., Stevanović, D. D., Masnikosa, R., Vraneš, M., Tot, A., Zarić, M. M., Marković-Simović, B., Misirkić-Marjanović, M., Vučićević, L., Savić, M., Jakovljević, V. Lj., Trajković, V. S., Volarević, V., Kanjevac, T.,& Rilak Simović, A.. (2018). Newly Synthesized Heteronuclear Ruthenium(II)/Ferrocene Complexes Suppress the Growth of Mammary Carcinoma in 4T1-Treated BALB/c Mice by Promoting Activation of Antitumor Immunity. in Organometallics, 37(22), 4250-4266.
https://doi.org/10.1021/acs.organomet.8b00604

Milutinović MM, Čanović PP, Stevanović DD, Masnikosa R, Vraneš M, Tot A, Zarić MM, Marković-Simović B, Misirkić-Marjanović M, Vučićević L, Savić M, Jakovljević VL, Trajković VS, Volarević V, Kanjevac T, Rilak Simović A. Newly Synthesized Heteronuclear Ruthenium(II)/Ferrocene Complexes Suppress the Growth of Mammary Carcinoma in 4T1-Treated BALB/c Mice by Promoting Activation of Antitumor Immunity. in Organometallics. 2018;37(22):4250-4266.
doi:10.1021/acs.organomet.8b00604 .

Milutinović, Milan M., Čanović, Petar P., Stevanović, Dragana D., Masnikosa, Romana, Vraneš, Milan, Tot, Aleksandar, Zarić, Milan M., Marković-Simović, Bojana, Misirkić-Marjanović, Maja, Vučićević, Ljubica, Savić, Maja, Jakovljević, Vladimir Lj., Trajković, Vladimir S., Volarević, Vladislav, Kanjevac, Tatjana, Rilak Simović, Ana, "Newly Synthesized Heteronuclear Ruthenium(II)/Ferrocene Complexes Suppress the Growth of Mammary Carcinoma in 4T1-Treated BALB/c Mice by Promoting Activation of Antitumor Immunity" in Organometallics, 37, no. 22 (2018):4250-4266,
https://doi.org/10.1021/acs.organomet.8b00604 . .

TY  - CONF
AU  - Đurić, Dragan
AU  - Mićović, Z.
AU  - Stamenković, A.
AU  - Jakovljević Uzelac, J.
AU  - Stojanović, M.
AU  - Šćepanović, Lj.
AU  - Mitrović, D.
AU  - Labudović Borović. M.
AU  - Krstić, Danijela
AU  - Čolović, Mirjana
AU  - Obrenović, R.
AU  - Hadžibegović, A.
AU  - Kostić, S.
AU  - Šobot, T.
AU  - Jakovljević, Vladimir
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12985
C3  - Joint meeting of national physiological societies "New perspectives in physiological research – young investigator forum"
T1  - Comparison of the effects of different sulfur amino acids on cardiovascular system in male wistar rats
SP  - 36
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12985
ER  - 

@conference{
author = "Đurić, Dragan and Mićović, Z. and Stamenković, A. and Jakovljević Uzelac, J. and Stojanović, M. and Šćepanović, Lj. and Mitrović, D. and Labudović Borović. M. and Krstić, Danijela and Čolović, Mirjana and Obrenović, R. and Hadžibegović, A. and Kostić, S. and Šobot, T. and Jakovljević, Vladimir",
year = "2017",
journal = "Joint meeting of national physiological societies "New perspectives in physiological research – young investigator forum"",
title = "Comparison of the effects of different sulfur amino acids on cardiovascular system in male wistar rats",
pages = "36-36",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12985"
}

Đurić, D., Mićović, Z., Stamenković, A., Jakovljević Uzelac, J., Stojanović, M., Šćepanović, Lj., Mitrović, D., Labudović Borović. M., Krstić, D., Čolović, M., Obrenović, R., Hadžibegović, A., Kostić, S., Šobot, T.,& Jakovljević, V.. (2017). Comparison of the effects of different sulfur amino acids on cardiovascular system in male wistar rats. in Joint meeting of national physiological societies "New perspectives in physiological research – young investigator forum", 36-36.
https://hdl.handle.net/21.15107/rcub_vinar_12985

Đurić D, Mićović Z, Stamenković A, Jakovljević Uzelac J, Stojanović M, Šćepanović L, Mitrović D, Labudović Borović. M., Krstić D, Čolović M, Obrenović R, Hadžibegović A, Kostić S, Šobot T, Jakovljević V. Comparison of the effects of different sulfur amino acids on cardiovascular system in male wistar rats. in Joint meeting of national physiological societies "New perspectives in physiological research – young investigator forum". 2017;:36-36.
https://hdl.handle.net/21.15107/rcub_vinar_12985 .

Đurić, Dragan, Mićović, Z., Stamenković, A., Jakovljević Uzelac, J., Stojanović, M., Šćepanović, Lj., Mitrović, D., Labudović Borović. M., Krstić, Danijela, Čolović, Mirjana, Obrenović, R., Hadžibegović, A., Kostić, S., Šobot, T., Jakovljević, Vladimir, "Comparison of the effects of different sulfur amino acids on cardiovascular system in male wistar rats" in Joint meeting of national physiological societies "New perspectives in physiological research – young investigator forum" (2017):36-36,
https://hdl.handle.net/21.15107/rcub_vinar_12985 .

TY  - JOUR
AU  - Spasojević-Tišma, Vera D.
AU  - Matović, Milovan
AU  - Mihaljević, Olgica B.
AU  - Živančević-Simonović, Snežana T.
AU  - Jeremic, Marija Z.
AU  - Jakovljević, Vladimir Lj.
AU  - Todorović, Vera N.
AU  - Pavlović, Ivan
AU  - Pejić, Snežana
AU  - Todorović, Ana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1935
AB  - The radioactive iodine (I-131) ablation is a well-accepted treatment modality for differentiated thyroid cancer patients. Unfortunately, the radiation induces the oxidative stress and damages cells and tissues, simultaneously activating the mechanisms of antioxidative defense. Since the mechanisms of those processes are not completely known, we wanted to examine the changes in the most important reactive oxygen species and antioxidative components, as well as their correlation and significance for lipid peroxidation. Our results showed that the level of thiobarbituric acid reactive substances was increased during the first 30 days after the radiotherapy. Among antioxidant components, superoxide dismutase was increased in the 3rd and 30th day; catalase in 7th and reduced glutathione in 3rd and 7th day after the radiotherapy. As regards the prooxidants, the reduction of hydrogen peroxide (H2O2) was recorded in 7th and 30th day, and superoxide anion radical (O-2(center dot-)) was unchanged after the exposure to I-131. These results indicate that differentiated thyroid cancer patients are under constant oxidative stress despite the observed increase in antioxidative and reduction in prooxidative parameters. The understanding of these early processes is important since their progress determines the latter effects of I-131 therapy.
T2  - Nuclear technology and radiation protection
T1  - Redox Parameters in Blood of Thyroid Cancer Patients After the Radioiodine Ablation
VL  - 32
IS  - 4
SP  - 358
EP  - 365
DO  - 10.2298/NTRP1704358S
ER  - 

@article{
author = "Spasojević-Tišma, Vera D. and Matović, Milovan and Mihaljević, Olgica B. and Živančević-Simonović, Snežana T. and Jeremic, Marija Z. and Jakovljević, Vladimir Lj. and Todorović, Vera N. and Pavlović, Ivan and Pejić, Snežana and Todorović, Ana",
year = "2017",
abstract = "The radioactive iodine (I-131) ablation is a well-accepted treatment modality for differentiated thyroid cancer patients. Unfortunately, the radiation induces the oxidative stress and damages cells and tissues, simultaneously activating the mechanisms of antioxidative defense. Since the mechanisms of those processes are not completely known, we wanted to examine the changes in the most important reactive oxygen species and antioxidative components, as well as their correlation and significance for lipid peroxidation. Our results showed that the level of thiobarbituric acid reactive substances was increased during the first 30 days after the radiotherapy. Among antioxidant components, superoxide dismutase was increased in the 3rd and 30th day; catalase in 7th and reduced glutathione in 3rd and 7th day after the radiotherapy. As regards the prooxidants, the reduction of hydrogen peroxide (H2O2) was recorded in 7th and 30th day, and superoxide anion radical (O-2(center dot-)) was unchanged after the exposure to I-131. These results indicate that differentiated thyroid cancer patients are under constant oxidative stress despite the observed increase in antioxidative and reduction in prooxidative parameters. The understanding of these early processes is important since their progress determines the latter effects of I-131 therapy.",
journal = "Nuclear technology and radiation protection",
title = "Redox Parameters in Blood of Thyroid Cancer Patients After the Radioiodine Ablation",
volume = "32",
number = "4",
pages = "358-365",
doi = "10.2298/NTRP1704358S"
}

Spasojević-Tišma, V. D., Matović, M., Mihaljević, O. B., Živančević-Simonović, S. T., Jeremic, M. Z., Jakovljević, V. Lj., Todorović, V. N., Pavlović, I., Pejić, S.,& Todorović, A.. (2017). Redox Parameters in Blood of Thyroid Cancer Patients After the Radioiodine Ablation. in Nuclear technology and radiation protection, 32(4), 358-365.
https://doi.org/10.2298/NTRP1704358S

Spasojević-Tišma VD, Matović M, Mihaljević OB, Živančević-Simonović ST, Jeremic MZ, Jakovljević VL, Todorović VN, Pavlović I, Pejić S, Todorović A. Redox Parameters in Blood of Thyroid Cancer Patients After the Radioiodine Ablation. in Nuclear technology and radiation protection. 2017;32(4):358-365.
doi:10.2298/NTRP1704358S .

Spasojević-Tišma, Vera D., Matović, Milovan, Mihaljević, Olgica B., Živančević-Simonović, Snežana T., Jeremic, Marija Z., Jakovljević, Vladimir Lj., Todorović, Vera N., Pavlović, Ivan, Pejić, Snežana, Todorović, Ana, "Redox Parameters in Blood of Thyroid Cancer Patients After the Radioiodine Ablation" in Nuclear technology and radiation protection, 32, no. 4 (2017):358-365,
https://doi.org/10.2298/NTRP1704358S . .

TY  - CONF
AU  - Đurić, Dragan
AU  - Krstić, Danijela
AU  - Čolović, Mirjana
AU  - Jakovljević, Jovana
AU  - Jakovljević, Vladimir
AU  - Tasić, Nebojša
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12983
AB  - L-methionine, the principal sulfur-containing amino acid in proteins, has important roles in cell physiology as an antioxidant, and in the breakdown of fats and heavy metals. Methionine is the precursor of homocysteine, and participates in the methylation and transsulfuration pathways. Elevated total plasma homocysteine (hyperhomocysteinemia) is associated with atherosclerosis, thromboembolic disease and cancer. Whether homocysteine per se or a coincident metabolic abnormality (homocysteine-related compounds, thiolactone metabolites) causes vascular disease is still an open question. Animals with genetic hyperhomocysteinemia have so far not displayed atheromatous lesions. However, when methionine-rich diets are used to induce hyperhomocysteinemia, vascular pathology is often observed. Such studies have not distinguished the effects of excess dietary methionine from those of hyperhomocysteinemia. It is known that high methionine diet, not only red meat for example lamb, beef, pork but also chicken meat can induce cardiovascular dysfunction but the mechanisms are unclear. It has been hypothesized that a diet rich in methionine can malfunction the cardiovascular system in three ways: (1) by augmenting oxidative stress, (2) by inflammatory manifestations, and (3) by matrix/vascular remodeling. However, some evidence indicates that an excess of methionine can be harmful for other systems, and can increase the risk of developing type-2 diabetes, certain types of cancer, brain alterations such as schizophrenia, and memory impairment. However this is still controversial because previous studies suggesting the use of L-methionine as a treatment for depression and other diseases indicate that it might also improve memory (role in brain function). Thus, the direction of our research is to further elucidate mechanisms of cardiovascular and neural effects of homocysteine vs. methionine overload.
PB  - Kragujevac : Medical Faculty
C3  - Serbian Journal of Experimental and Clinical Research
T1  - Methionine versus homocysteine: future directions in cardiovascular research
VL  - 18
IS  - 1
SP  - 39
EP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12983
ER  - 

@conference{
author = "Đurić, Dragan and Krstić, Danijela and Čolović, Mirjana and Jakovljević, Jovana and Jakovljević, Vladimir and Tasić, Nebojša",
year = "2016",
abstract = "L-methionine, the principal sulfur-containing amino acid in proteins, has important roles in cell physiology as an antioxidant, and in the breakdown of fats and heavy metals. Methionine is the precursor of homocysteine, and participates in the methylation and transsulfuration pathways. Elevated total plasma homocysteine (hyperhomocysteinemia) is associated with atherosclerosis, thromboembolic disease and cancer. Whether homocysteine per se or a coincident metabolic abnormality (homocysteine-related compounds, thiolactone metabolites) causes vascular disease is still an open question. Animals with genetic hyperhomocysteinemia have so far not displayed atheromatous lesions. However, when methionine-rich diets are used to induce hyperhomocysteinemia, vascular pathology is often observed. Such studies have not distinguished the effects of excess dietary methionine from those of hyperhomocysteinemia. It is known that high methionine diet, not only red meat for example lamb, beef, pork but also chicken meat can induce cardiovascular dysfunction but the mechanisms are unclear. It has been hypothesized that a diet rich in methionine can malfunction the cardiovascular system in three ways: (1) by augmenting oxidative stress, (2) by inflammatory manifestations, and (3) by matrix/vascular remodeling. However, some evidence indicates that an excess of methionine can be harmful for other systems, and can increase the risk of developing type-2 diabetes, certain types of cancer, brain alterations such as schizophrenia, and memory impairment. However this is still controversial because previous studies suggesting the use of L-methionine as a treatment for depression and other diseases indicate that it might also improve memory (role in brain function). Thus, the direction of our research is to further elucidate mechanisms of cardiovascular and neural effects of homocysteine vs. methionine overload.",
publisher = "Kragujevac : Medical Faculty",
journal = "Serbian Journal of Experimental and Clinical Research",
title = "Methionine versus homocysteine: future directions in cardiovascular research",
volume = "18",
number = "1",
pages = "39-39",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12983"
}

Đurić, D., Krstić, D., Čolović, M., Jakovljević, J., Jakovljević, V.,& Tasić, N.. (2016). Methionine versus homocysteine: future directions in cardiovascular research. in Serbian Journal of Experimental and Clinical Research
Kragujevac : Medical Faculty., 18(1), 39-39.
https://hdl.handle.net/21.15107/rcub_vinar_12983

Đurić D, Krstić D, Čolović M, Jakovljević J, Jakovljević V, Tasić N. Methionine versus homocysteine: future directions in cardiovascular research. in Serbian Journal of Experimental and Clinical Research. 2016;18(1):39-39.
https://hdl.handle.net/21.15107/rcub_vinar_12983 .

Đurić, Dragan, Krstić, Danijela, Čolović, Mirjana, Jakovljević, Jovana, Jakovljević, Vladimir, Tasić, Nebojša, "Methionine versus homocysteine: future directions in cardiovascular research" in Serbian Journal of Experimental and Clinical Research, 18, no. 1 (2016):39-39,
https://hdl.handle.net/21.15107/rcub_vinar_12983 .