Žakula, Jelena

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Authority KeyName Variants
orcid::0000-0001-7280-3273
  • Žakula, Jelena (15)
Projects
Radiosensitivity of human genome High Energy Physics with the CMS Detector
Mechanistic studies of the reactions of transition metal ion complexes with biologically relevant molecules Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, Italy
Ministry of Science and Technological Development of Serbia [143044, 141038], Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, Italy ARDITI [M1420-01-0145-FEDER-000005]
European Commission [2019093770] ENSAR - European Nuclear Science and Applications Research
Size-, shape- and structure- dependent properties of nanoparticles and nanocomposites Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, Catania, Italy
Junta de Andalucia [UMA18-FEDERJA-126] Ministerio de Ciencia, Innovacion y Universidades [RTI2018-099668-BC22]
National Laboratories of the South, National Institute for Nuclear Physics, Catania, Italy Portuguese Foundation for Science and Technology [PEstOE/QUI/UI0674/2019, UID/MAT/00006/2019, INNOINDIGO/0001/2015, RAM-M1420-01-0145-FEDER-000008]
Serbian-Chinese bilateral project [451-00-478/2018-09/16] Serbian-Chinese bilateral project [SINO-SER-BIA2018002]

Author's Bibliography

SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives

Nešić, Maja D.; Dučić, Tanja; Liang, Xinyue; Algarra, Manuel; Mi, Lan; Korićanac, Lela; Žakula, Jelena; Kop, Tatjana J.; Bjelaković, Mira S.; Mitrović, Aleksandra; Gojgić Cvijović, Gordana D.; Stepić, Milutin; Petković, Marijana

(2020)

TY  - JOUR
AU  - Nešić, Maja D.
AU  - Dučić, Tanja
AU  - Liang, Xinyue
AU  - Algarra, Manuel
AU  - Mi, Lan
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Kop, Tatjana J.
AU  - Bjelaković, Mira S.
AU  - Mitrović, Aleksandra
AU  - Gojgić Cvijović, Gordana D.
AU  - Stepić, Milutin
AU  - Petković, Marijana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9712
AB  - Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide I band, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60 potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.
T2  - International Journal of Biological Macromolecules
T1  - SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives
VL  - 165
SP  - 2541
EP  - 2549
DO  - 10.1016/j.ijbiomac.2020.10.141
ER  - 
@article{
author = "Nešić, Maja D. and Dučić, Tanja and Liang, Xinyue and Algarra, Manuel and Mi, Lan and Korićanac, Lela and Žakula, Jelena and Kop, Tatjana J. and Bjelaković, Mira S. and Mitrović, Aleksandra and Gojgić Cvijović, Gordana D. and Stepić, Milutin and Petković, Marijana",
year = "2020",
abstract = "Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide I band, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60 potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.",
journal = "International Journal of Biological Macromolecules",
title = "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives",
volume = "165",
pages = "2541-2549",
doi = "10.1016/j.ijbiomac.2020.10.141"
}
Nešić, M. D., Dučić, T., Liang, X., Algarra, M., Mi, L., Korićanac, L., Žakula, J., Kop, T. J., Bjelaković, M. S., Mitrović, A., Gojgić Cvijović, G. D., Stepić, M.,& Petković, M.. (2020). SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules, 165, 2541-2549.
https://doi.org/10.1016/j.ijbiomac.2020.10.141
Nešić MD, Dučić T, Liang X, Algarra M, Mi L, Korićanac L, Žakula J, Kop TJ, Bjelaković MS, Mitrović A, Gojgić Cvijović GD, Stepić M, Petković M. SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules. 2020;165:2541-2549.
doi:10.1016/j.ijbiomac.2020.10.141 .
Nešić, Maja D., Dučić, Tanja, Liang, Xinyue, Algarra, Manuel, Mi, Lan, Korićanac, Lela, Žakula, Jelena, Kop, Tatjana J., Bjelaković, Mira S., Mitrović, Aleksandra, Gojgić Cvijović, Gordana D., Stepić, Milutin, Petković, Marijana, "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives" in International Journal of Biological Macromolecules, 165 (2020):2541-2549,
https://doi.org/10.1016/j.ijbiomac.2020.10.141 . .
1
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1

Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex

Nešić, Maja A.; Žakula, Jelena; Korićanac, Lela; Stepić, Milutin; Radoičić, Marija B.; Popović, Iva A.; Šaponjić, Zoran; Petković, Marijana

(2017)

TY  - JOUR
AU  - Nešić, Maja A.
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Stepić, Milutin
AU  - Radoičić, Marija B.
AU  - Popović, Iva A.
AU  - Šaponjić, Zoran
AU  - Petković, Marijana
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1747
AB  - We studied the colloidal TiO2 nanoparticles as a carrier for controlled delivery of the ruthenium complex to the melanoma cell line. The system demonstrated slower complex release upon visible and increased release rate upon UV light illumination. Accordingly, the light-dependent cytotoxicity of the system was demonstrated on amelanotic melanoma cancer line. The cell death is enhanced by UV and reduced by red light in the presence of investigated nanocomposite system. Both components of the system may act as photosensitizers, by generating reactive oxygen species, which promote cell death. Thus, the system might act dually, as photodynamic therapeutic agent and as the light tunable system for metallo-drug delivery and it might be of interest for development of new more efficient drug delivery approaches by using a light as external stimulus. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Journal of Photochemistry and Photobiology. A: Chemistry
T1  - Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex
VL  - 347
SP  - 55
EP  - 66
DO  - 10.1016/jjphotochem.2017.06.045
ER  - 
@article{
author = "Nešić, Maja A. and Žakula, Jelena and Korićanac, Lela and Stepić, Milutin and Radoičić, Marija B. and Popović, Iva A. and Šaponjić, Zoran and Petković, Marijana",
year = "2017",
abstract = "We studied the colloidal TiO2 nanoparticles as a carrier for controlled delivery of the ruthenium complex to the melanoma cell line. The system demonstrated slower complex release upon visible and increased release rate upon UV light illumination. Accordingly, the light-dependent cytotoxicity of the system was demonstrated on amelanotic melanoma cancer line. The cell death is enhanced by UV and reduced by red light in the presence of investigated nanocomposite system. Both components of the system may act as photosensitizers, by generating reactive oxygen species, which promote cell death. Thus, the system might act dually, as photodynamic therapeutic agent and as the light tunable system for metallo-drug delivery and it might be of interest for development of new more efficient drug delivery approaches by using a light as external stimulus. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Journal of Photochemistry and Photobiology. A: Chemistry",
title = "Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex",
volume = "347",
pages = "55-66",
doi = "10.1016/jjphotochem.2017.06.045"
}
Nešić, M. A., Žakula, J., Korićanac, L., Stepić, M., Radoičić, M. B., Popović, I. A., Šaponjić, Z.,& Petković, M.. (2017). Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex. in Journal of Photochemistry and Photobiology. A: Chemistry, 347, 55-66.
https://doi.org/10.1016/jjphotochem.2017.06.045
Nešić MA, Žakula J, Korićanac L, Stepić M, Radoičić MB, Popović IA, Šaponjić Z, Petković M. Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex. in Journal of Photochemistry and Photobiology. A: Chemistry. 2017;347:55-66.
doi:10.1016/jjphotochem.2017.06.045 .
Nešić, Maja A., Žakula, Jelena, Korićanac, Lela, Stepić, Milutin, Radoičić, Marija B., Popović, Iva A., Šaponjić, Zoran, Petković, Marijana, "Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex" in Journal of Photochemistry and Photobiology. A: Chemistry, 347 (2017):55-66,
https://doi.org/10.1016/jjphotochem.2017.06.045 . .
9

Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells

Žakula, Jelena; Korićanac, Lela; Keta, Otilija D.; Todorović, Danijela V.; Cirrone, Giuseppe Antonio Pablo; Romano, Francesco; Cuttone, Giacomo; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(2016)

TY  - JOUR
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1290
AB  - Background and objectives: The main goal when treating malignancies with radiation is to deprive tumour cells of their reproductive potential. One approach is to induce tumour cell apoptosis. This study was conducted to evaluate the ability of carbon ions (C-12) to induce apoptosis and cell cycle arrest in human HTB140 melanoma cells. Methods: In this in vitro study, human melanoma HTB140 cells were irradiated with the 62 MeV/n carbon (C-12) ion beam, having two different linear energy transfer (LET) values: 197 and 382 keV/mu m. The dose range was 2 to 16 Gy. Cell viability was estimated by the sulforhodamine B assay seven days after irradiation. The cell cycle and apoptosis were evaluated 48 h after irradiation using flow cytometry. At the same time point, protein and gene expression of apoptotic regulators were estimated using the Western blot and q-PCR methods, respectively. Results: Cell viability experiments indicated strong anti-tumour effects of C-12 ions. The analysis of cell cycle showed that C-12 ions blocked HTB140 cells in G2 phase and induced the dose dependent increase of apoptosis. The maximum value of 21.8 per cent was attained after irradiation with LET of 197 keV/mu m at the dose level of 16 Gy. Pro-apoptotic effects of C-12 ions were confirmed by changes of key apoptotic molecules: the p53, Bax, Bcl-2, poly ADP ribose polymerase (PARP) as well as nuclear factor kappa B (NF kappa B). At the level of protein expression, the results indicated significant increases of p53, NF kappa B and Bax/Bcl-2 ratio and PARP cleavage. The Bax/Bcl-2 mRNA ratio was also increased, while no change was detected in the level of NF kappa B mRNA. Interpretation and conclusions: The present results indicated that anti-tumour effects of C-12 ions in human melanoma HTB140 cells were accomplished through induction of the mitochondrial apoptotic pathway as well as G2 arrest.
T2  - Indian Journal of Medical Research
T1  - Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells
VL  - 143
SP  - 120
EP  - 128
DO  - 10.4103/0971-5916.191811
ER  - 
@article{
author = "Žakula, Jelena and Korićanac, Lela and Keta, Otilija D. and Todorović, Danijela V. and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2016",
abstract = "Background and objectives: The main goal when treating malignancies with radiation is to deprive tumour cells of their reproductive potential. One approach is to induce tumour cell apoptosis. This study was conducted to evaluate the ability of carbon ions (C-12) to induce apoptosis and cell cycle arrest in human HTB140 melanoma cells. Methods: In this in vitro study, human melanoma HTB140 cells were irradiated with the 62 MeV/n carbon (C-12) ion beam, having two different linear energy transfer (LET) values: 197 and 382 keV/mu m. The dose range was 2 to 16 Gy. Cell viability was estimated by the sulforhodamine B assay seven days after irradiation. The cell cycle and apoptosis were evaluated 48 h after irradiation using flow cytometry. At the same time point, protein and gene expression of apoptotic regulators were estimated using the Western blot and q-PCR methods, respectively. Results: Cell viability experiments indicated strong anti-tumour effects of C-12 ions. The analysis of cell cycle showed that C-12 ions blocked HTB140 cells in G2 phase and induced the dose dependent increase of apoptosis. The maximum value of 21.8 per cent was attained after irradiation with LET of 197 keV/mu m at the dose level of 16 Gy. Pro-apoptotic effects of C-12 ions were confirmed by changes of key apoptotic molecules: the p53, Bax, Bcl-2, poly ADP ribose polymerase (PARP) as well as nuclear factor kappa B (NF kappa B). At the level of protein expression, the results indicated significant increases of p53, NF kappa B and Bax/Bcl-2 ratio and PARP cleavage. The Bax/Bcl-2 mRNA ratio was also increased, while no change was detected in the level of NF kappa B mRNA. Interpretation and conclusions: The present results indicated that anti-tumour effects of C-12 ions in human melanoma HTB140 cells were accomplished through induction of the mitochondrial apoptotic pathway as well as G2 arrest.",
journal = "Indian Journal of Medical Research",
title = "Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells",
volume = "143",
pages = "120-128",
doi = "10.4103/0971-5916.191811"
}
Žakula, J., Korićanac, L., Keta, O. D., Todorović, D. V., Cirrone, G. A. P., Romano, F., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2016). Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells. in Indian Journal of Medical Research, 143, 120-128.
https://doi.org/10.4103/0971-5916.191811
Žakula J, Korićanac L, Keta OD, Todorović DV, Cirrone GAP, Romano F, Cuttone G, Petrović IM, Ristić-Fira A. Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells. in Indian Journal of Medical Research. 2016;143:120-128.
doi:10.4103/0971-5916.191811 .
Žakula, Jelena, Korićanac, Lela, Keta, Otilija D., Todorović, Danijela V., Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells" in Indian Journal of Medical Research, 143 (2016):120-128,
https://doi.org/10.4103/0971-5916.191811 . .
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Radiation dose determines the method for quantification of DNA double strand breaks

Bulat, Tanja M.; Keta, Otilija D.; Korićanac, Lela; Žakula, Jelena; Petrović, Ivan M.; Ristić-Fira, Aleksandra; Todorović, Danijela V.

(2016)

TY  - JOUR
AU  - Bulat, Tanja M.
AU  - Keta, Otilija D.
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/970
AB  - Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (gamma H2AX). Immunofluorescent staining visualizes formation of gamma H2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position and intensity of fluorescent signal in each single cell. In practice there are problems in quantification of gamma H2AX. This paper is based on two issues: the determination of which technique should be applied concerning the radiation dose, and how to analyze fluorescent microscopy images obtained by different microscopes. HTB140 melanoma cells were exposed to gamma-rays, in the dose range from 1 to 16 Gy. Radiation effects on the DNA level were analyzed at different time intervals after irradiation by Western blot analysis and immunofluorescence microscopy. Immunochemically stained cells were visualized with two types of microscopes: AxioVision (Zeiss, Germany) microscope, comprising an ApoTome software, and AxioImagerA1 microscope (Zeiss, Germany). Obtained results show that the level of gamma H2AX is time and dose dependent. Immunofluorescence microscopy provided better detection of DSBs for lower irradiation doses, while Western blot analysis was more reliable for higher irradiation doses. AxioVision microscope containing ApoTome software was more suitable for the detection of gamma H2AX foci.
T2  - Anais de Academia Brasileira de Ciencias
T1  - Radiation dose determines the method for quantification of DNA double strand breaks
VL  - 88
IS  - 1
SP  - 127
EP  - 136
DO  - 10.1590/0001-3765201620140553
ER  - 
@article{
author = "Bulat, Tanja M. and Keta, Otilija D. and Korićanac, Lela and Žakula, Jelena and Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V.",
year = "2016",
abstract = "Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (gamma H2AX). Immunofluorescent staining visualizes formation of gamma H2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position and intensity of fluorescent signal in each single cell. In practice there are problems in quantification of gamma H2AX. This paper is based on two issues: the determination of which technique should be applied concerning the radiation dose, and how to analyze fluorescent microscopy images obtained by different microscopes. HTB140 melanoma cells were exposed to gamma-rays, in the dose range from 1 to 16 Gy. Radiation effects on the DNA level were analyzed at different time intervals after irradiation by Western blot analysis and immunofluorescence microscopy. Immunochemically stained cells were visualized with two types of microscopes: AxioVision (Zeiss, Germany) microscope, comprising an ApoTome software, and AxioImagerA1 microscope (Zeiss, Germany). Obtained results show that the level of gamma H2AX is time and dose dependent. Immunofluorescence microscopy provided better detection of DSBs for lower irradiation doses, while Western blot analysis was more reliable for higher irradiation doses. AxioVision microscope containing ApoTome software was more suitable for the detection of gamma H2AX foci.",
journal = "Anais de Academia Brasileira de Ciencias",
title = "Radiation dose determines the method for quantification of DNA double strand breaks",
volume = "88",
number = "1",
pages = "127-136",
doi = "10.1590/0001-3765201620140553"
}
Bulat, T. M., Keta, O. D., Korićanac, L., Žakula, J., Petrović, I. M., Ristić-Fira, A.,& Todorović, D. V.. (2016). Radiation dose determines the method for quantification of DNA double strand breaks. in Anais de Academia Brasileira de Ciencias, 88(1), 127-136.
https://doi.org/10.1590/0001-3765201620140553
Bulat TM, Keta OD, Korićanac L, Žakula J, Petrović IM, Ristić-Fira A, Todorović DV. Radiation dose determines the method for quantification of DNA double strand breaks. in Anais de Academia Brasileira de Ciencias. 2016;88(1):127-136.
doi:10.1590/0001-3765201620140553 .
Bulat, Tanja M., Keta, Otilija D., Korićanac, Lela, Žakula, Jelena, Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., "Radiation dose determines the method for quantification of DNA double strand breaks" in Anais de Academia Brasileira de Ciencias, 88, no. 1 (2016):127-136,
https://doi.org/10.1590/0001-3765201620140553 . .
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New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity

Jovanović, Snežana; Obrencevic, Katarina; Bugarčić, Živadin D.; Popović, Iva A.; Žakula, Jelena; Petrović, Biljana

(2016)

TY  - JOUR
AU  - Jovanović, Snežana
AU  - Obrencevic, Katarina
AU  - Bugarčić, Živadin D.
AU  - Popović, Iva A.
AU  - Žakula, Jelena
AU  - Petrović, Biljana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1208
AB  - Two new dinuclear bimetallic complexes, [{PdCl(bipy)}{mu-(pyrazine)}{PtCl(bipy)}]Cl(ClO4) (1) (bipy is 2,2-=bipyridine) and [{PdCl(en)}{mu-(pyrazine)}{PtCl(en)}]Cl(ClO4) (2) (en is ethylenediamine), have been synthesized and characterized by elemental microanalysis, IR, H-1 NMR spectroscopy and MALDI-TOF mass spectrometry. The pK(a) values of the coordinated water molecules of the diaqua species were determined as well. Substitution reactions of complexes (1) and (2) with thiourea (Tu), L-methionine (L-Met), L-cysteine (L-Cys), L-histidine (L-His) and guanosine-5-monophosphate (5-GMP) were studied under the pseudo-first order conditions as a function of nucleophile concentration and temperature. The order of reactivity of nucleophiles was: Tu GT L-Met GT L-Cys GT L-His GT 5-GMP. Substitution reactions with Tu, L-Cys and L-His were followed by decomposition of bimetallic complexes to the corresponding substituted mononuclear complexes [Pd(N-N)(Nu)(2)] and [Pt(N-N)(Nu)(2)] (N-N = bipy, en), releasing the bridging ligand. However, the structures of starting bimetallic complexes were preserved during the reactions with L-Met and 5-GMP. The absorption spectroscopic study of interactions of calf-thymus DNA (CT-DNA) with complexes (1), (2) and [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)} {PtCl(bipy)}] Cl(ClO4) (3), has shown that all the complexes exhibit high intrinsic binding constants (K-b = 10(4)-10(5) M-1). DNA-ethidium bromide (DNA-EB) fluorescence was quenched after addition of complexes (1), (2) or (3), indicating displacement of intercalating EB by complexes. All complexes have shown good binding affinity to bovine serum albumin protein (BSA). Chemosensitivity of A375 (human melanoma) and HeLa (human cervical cancer) cell lines toward complexes (1), (2) and (3) was analyzed by SRB assay. Complex (1) displayed significant inhibitory effect on the growth of both cell lines.
T2  - Dalton Transactions
T1  - New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity
VL  - 45
IS  - 31
SP  - 12444
EP  - 12457
DO  - 10.1039/c6dt02226j
ER  - 
@article{
author = "Jovanović, Snežana and Obrencevic, Katarina and Bugarčić, Živadin D. and Popović, Iva A. and Žakula, Jelena and Petrović, Biljana",
year = "2016",
abstract = "Two new dinuclear bimetallic complexes, [{PdCl(bipy)}{mu-(pyrazine)}{PtCl(bipy)}]Cl(ClO4) (1) (bipy is 2,2-=bipyridine) and [{PdCl(en)}{mu-(pyrazine)}{PtCl(en)}]Cl(ClO4) (2) (en is ethylenediamine), have been synthesized and characterized by elemental microanalysis, IR, H-1 NMR spectroscopy and MALDI-TOF mass spectrometry. The pK(a) values of the coordinated water molecules of the diaqua species were determined as well. Substitution reactions of complexes (1) and (2) with thiourea (Tu), L-methionine (L-Met), L-cysteine (L-Cys), L-histidine (L-His) and guanosine-5-monophosphate (5-GMP) were studied under the pseudo-first order conditions as a function of nucleophile concentration and temperature. The order of reactivity of nucleophiles was: Tu GT L-Met GT L-Cys GT L-His GT 5-GMP. Substitution reactions with Tu, L-Cys and L-His were followed by decomposition of bimetallic complexes to the corresponding substituted mononuclear complexes [Pd(N-N)(Nu)(2)] and [Pt(N-N)(Nu)(2)] (N-N = bipy, en), releasing the bridging ligand. However, the structures of starting bimetallic complexes were preserved during the reactions with L-Met and 5-GMP. The absorption spectroscopic study of interactions of calf-thymus DNA (CT-DNA) with complexes (1), (2) and [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)} {PtCl(bipy)}] Cl(ClO4) (3), has shown that all the complexes exhibit high intrinsic binding constants (K-b = 10(4)-10(5) M-1). DNA-ethidium bromide (DNA-EB) fluorescence was quenched after addition of complexes (1), (2) or (3), indicating displacement of intercalating EB by complexes. All complexes have shown good binding affinity to bovine serum albumin protein (BSA). Chemosensitivity of A375 (human melanoma) and HeLa (human cervical cancer) cell lines toward complexes (1), (2) and (3) was analyzed by SRB assay. Complex (1) displayed significant inhibitory effect on the growth of both cell lines.",
journal = "Dalton Transactions",
title = "New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity",
volume = "45",
number = "31",
pages = "12444-12457",
doi = "10.1039/c6dt02226j"
}
Jovanović, S., Obrencevic, K., Bugarčić, Ž. D., Popović, I. A., Žakula, J.,& Petrović, B.. (2016). New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity. in Dalton Transactions, 45(31), 12444-12457.
https://doi.org/10.1039/c6dt02226j
Jovanović S, Obrencevic K, Bugarčić ŽD, Popović IA, Žakula J, Petrović B. New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity. in Dalton Transactions. 2016;45(31):12444-12457.
doi:10.1039/c6dt02226j .
Jovanović, Snežana, Obrencevic, Katarina, Bugarčić, Živadin D., Popović, Iva A., Žakula, Jelena, Petrović, Biljana, "New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity" in Dalton Transactions, 45, no. 31 (2016):12444-12457,
https://doi.org/10.1039/c6dt02226j . .
1
26
31
31

Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition

Keta, Otilija D.; Bulat, Tanja M.; Korićanac, Lela; Žakula, Jelena; Cuttone, Giacomo; Privitera, Giuseppe; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(2014)

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Bulat, Tanja M.
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Cuttone, Giacomo
AU  - Privitera, Giuseppe
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/167
AB  - Molecular targeted cancer therapy is a promising treatment strategy. Considering the central role of the epidermal growth factor receptor in cell proliferation and survival, there are indications that targeted agents like tyrosine kinase inhibitors, i. e., erlotinib, may enhance the antitumor treatment by radiation. The aim of this study is to analyze the inactivation effects of gamma-rays and to test the radiosensitizing potential of erlotinib on human lung adenocarcinoma cells in vitro. Irradiations were performed with doses ranging from 1 Gy to 8 Gy. In order to increase the radiosensitivity of CRL-5876 lung adenocarcinoma cells, the cells were treated with a clinically relevant concentration of 2 mu M erlotinib. The effects of single and combined treatments were monitored using clonogenic survival, cell viability and proliferation assays at different time points. For the detection and visualization of the phosphorylated histone H2AX (gamma-H2AX), an important biological marker of DNA double-strand break formation, fluorescence inununocytochemistry, was performed. The response to the treatment was monitored at four time points: 30 min, 2, 6, and 24 h. Irradiations with gamma-rays resulted in significant cell inactivation regarding all analyzed biological endpoints. Combined treatments revealed consistent cell inactivation. Moreover, compared to gamma-rays alone, elevated levels of gamma-H2AX foci were observed after pretreatment with erlotinib, indicating radiosensitization through impaired DNA repair.
T2  - Nuclear technology and radiation protection
T1  - Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition
VL  - 29
IS  - 3
SP  - 233
EP  - 241
DO  - 10.2298/NTRP1403233K
ER  - 
@article{
author = "Keta, Otilija D. and Bulat, Tanja M. and Korićanac, Lela and Žakula, Jelena and Cuttone, Giacomo and Privitera, Giuseppe and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2014",
abstract = "Molecular targeted cancer therapy is a promising treatment strategy. Considering the central role of the epidermal growth factor receptor in cell proliferation and survival, there are indications that targeted agents like tyrosine kinase inhibitors, i. e., erlotinib, may enhance the antitumor treatment by radiation. The aim of this study is to analyze the inactivation effects of gamma-rays and to test the radiosensitizing potential of erlotinib on human lung adenocarcinoma cells in vitro. Irradiations were performed with doses ranging from 1 Gy to 8 Gy. In order to increase the radiosensitivity of CRL-5876 lung adenocarcinoma cells, the cells were treated with a clinically relevant concentration of 2 mu M erlotinib. The effects of single and combined treatments were monitored using clonogenic survival, cell viability and proliferation assays at different time points. For the detection and visualization of the phosphorylated histone H2AX (gamma-H2AX), an important biological marker of DNA double-strand break formation, fluorescence inununocytochemistry, was performed. The response to the treatment was monitored at four time points: 30 min, 2, 6, and 24 h. Irradiations with gamma-rays resulted in significant cell inactivation regarding all analyzed biological endpoints. Combined treatments revealed consistent cell inactivation. Moreover, compared to gamma-rays alone, elevated levels of gamma-H2AX foci were observed after pretreatment with erlotinib, indicating radiosensitization through impaired DNA repair.",
journal = "Nuclear technology and radiation protection",
title = "Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition",
volume = "29",
number = "3",
pages = "233-241",
doi = "10.2298/NTRP1403233K"
}
Keta, O. D., Bulat, T. M., Korićanac, L., Žakula, J., Cuttone, G., Privitera, G., Petrović, I. M.,& Ristić-Fira, A.. (2014). Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition. in Nuclear technology and radiation protection, 29(3), 233-241.
https://doi.org/10.2298/NTRP1403233K
Keta OD, Bulat TM, Korićanac L, Žakula J, Cuttone G, Privitera G, Petrović IM, Ristić-Fira A. Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition. in Nuclear technology and radiation protection. 2014;29(3):233-241.
doi:10.2298/NTRP1403233K .
Keta, Otilija D., Bulat, Tanja M., Korićanac, Lela, Žakula, Jelena, Cuttone, Giacomo, Privitera, Giuseppe, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition" in Nuclear technology and radiation protection, 29, no. 3 (2014):233-241,
https://doi.org/10.2298/NTRP1403233K . .
2
2
2

Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells

Korićanac, Lela; Žakula, Jelena; Keta, Otilija D.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Ristić-Fira, Aleksandra; Petrović, Ivan M.

(2013)

TY  - JOUR
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Keta, Otilija D.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5593
AB  - This study was conducted in order to evaluate the ability of carbon ions to induce DNA double-strand breaks and apoptosis in the radio-resistant human HTB140 melanoma cells. The cells were irradiated with C-12 ions having the linear energy transfer of 258 keV/mu m. Irradiations were performed in the dose range from 2 to 16 Gy. Induction of DNA double-strand breaks was evaluated 2 hour after irradiation through expression of gamma H2AX protein. Increased level of gamma H2AX detected in irradiated samples was especially high after irradiation with 12 and 16 Gy. Dose dependent increase of apoptosis was detected 48 hour after irradiation by flow-cytometry, with the maximum value of 20.4% after irradiation with 16 Gy, and the apoptotic index of 9.3. Pro-apoptotic effects of carbon ion beams were confirmed by changes of key molecules of the mitochondrial apoptotic pathway, p53 protein expression, Bax/Bcl-2 ratio and caspase-3 activation.
T2  - Nuclear technology and radiation protection
T1  - Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells
VL  - 28
IS  - 2
SP  - 195
EP  - 203
DO  - 10.2298/NTRP1302195K
ER  - 
@article{
author = "Korićanac, Lela and Žakula, Jelena and Keta, Otilija D. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra and Petrović, Ivan M.",
year = "2013",
abstract = "This study was conducted in order to evaluate the ability of carbon ions to induce DNA double-strand breaks and apoptosis in the radio-resistant human HTB140 melanoma cells. The cells were irradiated with C-12 ions having the linear energy transfer of 258 keV/mu m. Irradiations were performed in the dose range from 2 to 16 Gy. Induction of DNA double-strand breaks was evaluated 2 hour after irradiation through expression of gamma H2AX protein. Increased level of gamma H2AX detected in irradiated samples was especially high after irradiation with 12 and 16 Gy. Dose dependent increase of apoptosis was detected 48 hour after irradiation by flow-cytometry, with the maximum value of 20.4% after irradiation with 16 Gy, and the apoptotic index of 9.3. Pro-apoptotic effects of carbon ion beams were confirmed by changes of key molecules of the mitochondrial apoptotic pathway, p53 protein expression, Bax/Bcl-2 ratio and caspase-3 activation.",
journal = "Nuclear technology and radiation protection",
title = "Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells",
volume = "28",
number = "2",
pages = "195-203",
doi = "10.2298/NTRP1302195K"
}
Korićanac, L., Žakula, J., Keta, O. D., Cirrone, G. A. P., Cuttone, G., Ristić-Fira, A.,& Petrović, I. M.. (2013). Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells. in Nuclear technology and radiation protection, 28(2), 195-203.
https://doi.org/10.2298/NTRP1302195K
Korićanac L, Žakula J, Keta OD, Cirrone GAP, Cuttone G, Ristić-Fira A, Petrović IM. Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells. in Nuclear technology and radiation protection. 2013;28(2):195-203.
doi:10.2298/NTRP1302195K .
Korićanac, Lela, Žakula, Jelena, Keta, Otilija D., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells" in Nuclear technology and radiation protection, 28, no. 2 (2013):195-203,
https://doi.org/10.2298/NTRP1302195K . .
2
2
2

Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET

Petrović, Ivan M.; Ristić-Fira, Aleksandra; Todorović, Dragana; Korićanac, Lela; Žakula, Jelena; Cirrone, Giuseppe Antonio Pablo; Romano, Francesco; Cuttone, Giacomo

(2012)

TY  - CONF
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Dragana
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8643
C3  - Radiotherapy and Oncology
T1  - Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET
VL  - 102
SP  - S108
EP  - S109
DO  - 10.1016/S0167-8140(12)70185-8
ER  - 
@conference{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Dragana and Korićanac, Lela and Žakula, Jelena and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo",
year = "2012",
journal = "Radiotherapy and Oncology",
title = "Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET",
volume = "102",
pages = "S108-S109",
doi = "10.1016/S0167-8140(12)70185-8"
}
Petrović, I. M., Ristić-Fira, A., Todorović, D., Korićanac, L., Žakula, J., Cirrone, G. A. P., Romano, F.,& Cuttone, G.. (2012). Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET. in Radiotherapy and Oncology, 102, S108-S109.
https://doi.org/10.1016/S0167-8140(12)70185-8
Petrović IM, Ristić-Fira A, Todorović D, Korićanac L, Žakula J, Cirrone GAP, Romano F, Cuttone G. Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET. in Radiotherapy and Oncology. 2012;102:S108-S109.
doi:10.1016/S0167-8140(12)70185-8 .
Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Dragana, Korićanac, Lela, Žakula, Jelena, Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, "Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET" in Radiotherapy and Oncology, 102 (2012):S108-S109,
https://doi.org/10.1016/S0167-8140(12)70185-8 . .
1

Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line

Korićanac, Lela; Žakula, Jelena; Cirrone, Giuseppe Antonio Pablo; Privitera, Giuseppe; Cuttone, Giacomo; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(2012)

TY  - JOUR
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Privitera, Giuseppe
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8644
AB  - The effects of combined treatments with fotemustine and proton radiation on cell proliferation and induction of apoptosis have been analyzed in this study. HTB140 human melanoma cells were treated with fotemustine (100, 250 M) 24 h prior to irradiation (12, 16 Gy). The cells were irradiated in the middle of a therapeutic 62 MeV proton spread-out Bragg peak. An efficiency of applied treatments was observed throughout the evaluation of the cell proliferation 7 days after proton irradiation. The combined treatments with fotemustine and protons resulted in a greater antiproliferative response than each treatment alone. The number of apoptotic cells was estimated after 6 or 48 h using flow cytometry. The highest percentage of apoptotic cells was obtained 48 h after treatment with 250 M fotemustine and protons. Western blot analysis showed that induction of apoptosis was associated with p53 and Bax up regulation, and Bcl-2 down regulation. The induction of a caspase-3 activity and cleavage of PARP were clearly observed. These data indicate that a combined application of FM and proton irradiation is more effective in reducing melanoma cell proliferation and the induction of apoptosis, suggesting that FM can increase the radio-sensitivity of HTB140 melanoma cells.
T2  - Advanced Science Letters
T1  - Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line
VL  - 5
IS  - 2
SP  - 552
EP  - 559
DO  - 10.1166/asl.2012.2150
ER  - 
@article{
author = "Korićanac, Lela and Žakula, Jelena and Cirrone, Giuseppe Antonio Pablo and Privitera, Giuseppe and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2012",
abstract = "The effects of combined treatments with fotemustine and proton radiation on cell proliferation and induction of apoptosis have been analyzed in this study. HTB140 human melanoma cells were treated with fotemustine (100, 250 M) 24 h prior to irradiation (12, 16 Gy). The cells were irradiated in the middle of a therapeutic 62 MeV proton spread-out Bragg peak. An efficiency of applied treatments was observed throughout the evaluation of the cell proliferation 7 days after proton irradiation. The combined treatments with fotemustine and protons resulted in a greater antiproliferative response than each treatment alone. The number of apoptotic cells was estimated after 6 or 48 h using flow cytometry. The highest percentage of apoptotic cells was obtained 48 h after treatment with 250 M fotemustine and protons. Western blot analysis showed that induction of apoptosis was associated with p53 and Bax up regulation, and Bcl-2 down regulation. The induction of a caspase-3 activity and cleavage of PARP were clearly observed. These data indicate that a combined application of FM and proton irradiation is more effective in reducing melanoma cell proliferation and the induction of apoptosis, suggesting that FM can increase the radio-sensitivity of HTB140 melanoma cells.",
journal = "Advanced Science Letters",
title = "Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line",
volume = "5",
number = "2",
pages = "552-559",
doi = "10.1166/asl.2012.2150"
}
Korićanac, L., Žakula, J., Cirrone, G. A. P., Privitera, G., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2012). Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line. in Advanced Science Letters, 5(2), 552-559.
https://doi.org/10.1166/asl.2012.2150
Korićanac L, Žakula J, Cirrone GAP, Privitera G, Cuttone G, Petrović IM, Ristić-Fira A. Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line. in Advanced Science Letters. 2012;5(2):552-559.
doi:10.1166/asl.2012.2150 .
Korićanac, Lela, Žakula, Jelena, Cirrone, Giuseppe Antonio Pablo, Privitera, Giuseppe, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line" in Advanced Science Letters, 5, no. 2 (2012):552-559,
https://doi.org/10.1166/asl.2012.2150 . .
1
1

Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons

Ristić-Fira, Aleksandra; Todorović, Danijela V.; Žakula, Jelena; Keta, Otilija D.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Petrović, Ivan M.

(2011)

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Žakula, Jelena
AU  - Keta, Otilija D.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4537
AB  - Conventional radiotherapy with X-and gamma-rays is one of the common and effective treatments of cancer. High energy hadrons, i.e., charged particles like protons and (12)C ions, due to their specific physics and radiobiological advantages are increasingly used. In this study, effectiveness of different radiation types is evaluated on the radio-resistant human HTB140 melanoma cells. The cells were irradiated with gamma-rays, the 62 MeV protons at the Bragg peak and in the middle of the spread-out Bragg peak (SOBP), as well as with the 62 MeV/u (12)C ions. The doses ranged from 2 to 24 Gy. Cell survival and proliferation were assessed 7 days after irradiation, whereas apoptosis was evaluated after 48 h. The acquired results confirmed the high radio-resistance of cells, showing better effectiveness of protons than gamma-rays. The best efficiency was obtained with (12)C ions due to higher linear energy transfer. All analyzed radiation qualities reduced cell proliferation. The highest proliferation was detected for (12)C ions because of their large killing capacity followed by small induction of reparable lesions. This enabled unharmed cells to preserve proliferative activity. Irradiations with protons and (12)C ions revealed similar moderate pro-apoptotic ability that is in agreement with the level of cellular radio-resistance.
T2  - Physiological Research
T1  - Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons
VL  - 60
SP  - S129
EP  - S135
ER  - 
@article{
author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Žakula, Jelena and Keta, Otilija D. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2011",
abstract = "Conventional radiotherapy with X-and gamma-rays is one of the common and effective treatments of cancer. High energy hadrons, i.e., charged particles like protons and (12)C ions, due to their specific physics and radiobiological advantages are increasingly used. In this study, effectiveness of different radiation types is evaluated on the radio-resistant human HTB140 melanoma cells. The cells were irradiated with gamma-rays, the 62 MeV protons at the Bragg peak and in the middle of the spread-out Bragg peak (SOBP), as well as with the 62 MeV/u (12)C ions. The doses ranged from 2 to 24 Gy. Cell survival and proliferation were assessed 7 days after irradiation, whereas apoptosis was evaluated after 48 h. The acquired results confirmed the high radio-resistance of cells, showing better effectiveness of protons than gamma-rays. The best efficiency was obtained with (12)C ions due to higher linear energy transfer. All analyzed radiation qualities reduced cell proliferation. The highest proliferation was detected for (12)C ions because of their large killing capacity followed by small induction of reparable lesions. This enabled unharmed cells to preserve proliferative activity. Irradiations with protons and (12)C ions revealed similar moderate pro-apoptotic ability that is in agreement with the level of cellular radio-resistance.",
journal = "Physiological Research",
title = "Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons",
volume = "60",
pages = "S129-S135"
}
Ristić-Fira, A., Todorović, D. V., Žakula, J., Keta, O. D., Cirrone, G. A. P., Cuttone, G.,& Petrović, I. M.. (2011). Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons. in Physiological Research, 60, S129-S135.
Ristić-Fira A, Todorović DV, Žakula J, Keta OD, Cirrone GAP, Cuttone G, Petrović IM. Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons. in Physiological Research. 2011;60:S129-S135..
Ristić-Fira, Aleksandra, Todorović, Danijela V., Žakula, Jelena, Keta, Otilija D., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Petrović, Ivan M., "Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons" in Physiological Research, 60 (2011):S129-S135.
6

Proton Inactivation of Melanomacells Enhanced By Fotemustine

Ristić-Fira, Aleksandra; Korićanac, Lela; Žakula, Jelena; Keta, Otilija D.; Iannolo, Gioacchin; Cuttone, Giacomo; Petrović, Ivan M.

(2011)

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Keta, Otilija D.
AU  - Iannolo, Gioacchin
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6905
AB  - Response of human HTB140 melanoma cells to proton irradiation in combination with fotemustine (FM) was investigated. Effects of these agents were analysed on cell proliferation and induction of apoptosis. Cells pretreated with 100- or 250-mu M of FM were irradiated in the middle of the therapeutic 62-MeV proton spread-out Bragg peak, with a dose of 16 Gy. All treatments reduced proliferation and survival of melanoma cells. The most pronounced effects of the combined treatment were obtained for cell survivals. The level of apoptosis increased after all applied treatments. Particularly good pro-apoptotic effect was achieved when proton irradiation was combined with 250 mu M of FM. This was followed by the increased expression of p53 gene. The obtained results have shown that combined application of FM and protons significantly reduced growth of this resistant melanoma cell line.
T2  - Radiation Protection Dosimetry
T1  - Proton Inactivation of Melanomacells Enhanced By Fotemustine
VL  - 143
IS  - 2-4
SP  - 503
EP  - 507
DO  - 10.1093/rpd/ncq527
ER  - 
@article{
author = "Ristić-Fira, Aleksandra and Korićanac, Lela and Žakula, Jelena and Keta, Otilija D. and Iannolo, Gioacchin and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2011",
abstract = "Response of human HTB140 melanoma cells to proton irradiation in combination with fotemustine (FM) was investigated. Effects of these agents were analysed on cell proliferation and induction of apoptosis. Cells pretreated with 100- or 250-mu M of FM were irradiated in the middle of the therapeutic 62-MeV proton spread-out Bragg peak, with a dose of 16 Gy. All treatments reduced proliferation and survival of melanoma cells. The most pronounced effects of the combined treatment were obtained for cell survivals. The level of apoptosis increased after all applied treatments. Particularly good pro-apoptotic effect was achieved when proton irradiation was combined with 250 mu M of FM. This was followed by the increased expression of p53 gene. The obtained results have shown that combined application of FM and protons significantly reduced growth of this resistant melanoma cell line.",
journal = "Radiation Protection Dosimetry",
title = "Proton Inactivation of Melanomacells Enhanced By Fotemustine",
volume = "143",
number = "2-4",
pages = "503-507",
doi = "10.1093/rpd/ncq527"
}
Ristić-Fira, A., Korićanac, L., Žakula, J., Keta, O. D., Iannolo, G., Cuttone, G.,& Petrović, I. M.. (2011). Proton Inactivation of Melanomacells Enhanced By Fotemustine. in Radiation Protection Dosimetry, 143(2-4), 503-507.
https://doi.org/10.1093/rpd/ncq527
Ristić-Fira A, Korićanac L, Žakula J, Keta OD, Iannolo G, Cuttone G, Petrović IM. Proton Inactivation of Melanomacells Enhanced By Fotemustine. in Radiation Protection Dosimetry. 2011;143(2-4):503-507.
doi:10.1093/rpd/ncq527 .
Ristić-Fira, Aleksandra, Korićanac, Lela, Žakula, Jelena, Keta, Otilija D., Iannolo, Gioacchin, Cuttone, Giacomo, Petrović, Ivan M., "Proton Inactivation of Melanomacells Enhanced By Fotemustine" in Radiation Protection Dosimetry, 143, no. 2-4 (2011):503-507,
https://doi.org/10.1093/rpd/ncq527 . .
1
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2

Radio-sensitivity of human melanoma, ovarian and lung carcinoma cells to gamma radiation

Keta, Otilija D.; Korićanac, Lela; Žakula, Jelena; Popović, Nataša; Cuttone, Giacomo; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(Society of Physical Chemists of Serbia, 2010)

TY  - CONF
AU  - Keta, Otilija D.
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Popović, Nataša
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9314
AB  - Radio-sensitivity of human melanoma, ovarian and lung cancer cells after the
exposure to gamma-rays was studied using three different methods. The results
showed that gamma rays reduce the number of viable cells for all analyzed cell
lines. However, these cells display high level of radio-resistance. The highest
radio-sensitivity was attained for the CRL5876 lung cells, while the most sensitive
assay was the clonogenic assay.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Radio-sensitivity of human melanoma, ovarian and lung carcinoma cells to gamma radiation
ER  - 
@conference{
author = "Keta, Otilija D. and Korićanac, Lela and Žakula, Jelena and Popović, Nataša and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2010",
abstract = "Radio-sensitivity of human melanoma, ovarian and lung cancer cells after the
exposure to gamma-rays was studied using three different methods. The results
showed that gamma rays reduce the number of viable cells for all analyzed cell
lines. However, these cells display high level of radio-resistance. The highest
radio-sensitivity was attained for the CRL5876 lung cells, while the most sensitive
assay was the clonogenic assay.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Radio-sensitivity of human melanoma, ovarian and lung carcinoma cells to gamma radiation"
}
Keta, O. D., Korićanac, L., Žakula, J., Popović, N., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2010). Radio-sensitivity of human melanoma, ovarian and lung carcinoma cells to gamma radiation. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
Keta OD, Korićanac L, Žakula J, Popović N, Cuttone G, Petrović IM, Ristić-Fira A. Radio-sensitivity of human melanoma, ovarian and lung carcinoma cells to gamma radiation. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;..
Keta, Otilija D., Korićanac, Lela, Žakula, Jelena, Popović, Nataša, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Radio-sensitivity of human melanoma, ovarian and lung carcinoma cells to gamma radiation" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010).

Carbon ion beam as inducer of melanoma cell apoptosis

Žakula, Jelena; Korićanac, Lela; Keta, Otilija D.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Ristić-Fira, Aleksandra; Petrović, Ivan M.

(Society of Physical Chemists of Serbia, 2010)

TY  - CONF
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9325
AB  - In vitro effect of carbon ions on apoptosis was studied. The human melanoma
HTB140 cells were irradiated with the 62 MeV/u 12C ion beam. Percentage of
apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic
indexes were calculated. The expression of apoptosis-associated proteins, p53, Bax
and Bcl-2 was estimated by Western blot analyses. A dose dependent increase of
apoptosis was revealed, with the maximum value of 17 % after irradiation with 16
Gy, and the apoptotic index of 7.7. Pro-apoptotic effects of carbon ion beams were
confirmed by the detected changes of key regulators of the mitochondrial apoptotic
pathway, the p53 protein expression and the Bax/Bcl-2 ratio.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Carbon ion beam as inducer of melanoma cell apoptosis
ER  - 
@conference{
author = "Žakula, Jelena and Korićanac, Lela and Keta, Otilija D. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra and Petrović, Ivan M.",
year = "2010",
abstract = "In vitro effect of carbon ions on apoptosis was studied. The human melanoma
HTB140 cells were irradiated with the 62 MeV/u 12C ion beam. Percentage of
apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic
indexes were calculated. The expression of apoptosis-associated proteins, p53, Bax
and Bcl-2 was estimated by Western blot analyses. A dose dependent increase of
apoptosis was revealed, with the maximum value of 17 % after irradiation with 16
Gy, and the apoptotic index of 7.7. Pro-apoptotic effects of carbon ion beams were
confirmed by the detected changes of key regulators of the mitochondrial apoptotic
pathway, the p53 protein expression and the Bax/Bcl-2 ratio.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Carbon ion beam as inducer of melanoma cell apoptosis"
}
Žakula, J., Korićanac, L., Keta, O. D., Cirrone, G. A. P., Cuttone, G., Ristić-Fira, A.,& Petrović, I. M.. (2010). Carbon ion beam as inducer of melanoma cell apoptosis. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
Žakula J, Korićanac L, Keta OD, Cirrone GAP, Cuttone G, Ristić-Fira A, Petrović IM. Carbon ion beam as inducer of melanoma cell apoptosis. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;..
Žakula, Jelena, Korićanac, Lela, Keta, Otilija D., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Carbon ion beam as inducer of melanoma cell apoptosis" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010).

Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab

Korićanac, Lela; Žakula, Jelena; Petrović, Ivan M.; Valastro, Lucia M.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Ristić-Fira, Aleksandra

(2010)

TY  - JOUR
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Petrović, Ivan M.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
PY  - 2010
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4021
AB  - Background: Metastatic melanoma is one of the most aggressive tumours and is also very resistant to current therapeutic approaches. The aim of this investigation was the in vitro study of the anti-proliferative effects of fotemustine (FM; 100 and 250 mu M), bevacizumab (5 mu g/ml) and proton irradiation (12 and 16 Gy) on resistant HTB140 human melanoma cells. Methods: Viability was estimated by sulphorhodamine B assay, while cell proliferation was analyzed by 5-bromo-2-deoxyuridine assay. Cell cycle distribution and apoptosis were examined using flow cytometry. Results: Cell viability and proliferation were reduced after all applied treatments. The level of apoptosis significantly increased after treatment with FM, protons or a combination of all agents, while the apoptotic index ranged from 1.2 to 9.2. Proton irradiation, as well as combined treatment with bevacizumab and protons or 100 mu M FM, bevacizumab and protons, have reduced melanoma cell proliferation through the induction of G1 phase arrest. Single FM (250 mu M) or bevacizumab treatment and their combination, as well as the joint application of these 2 agents with protons, reduced cell proliferation and provoked G2 phase accumulation. Conclusion: The analyzed treatments reduced cell viability and proliferation, triggered G1 or G2 cell cycle phase accumulation and stimulated apoptotic cell death. Copyright (C) 2010 S. Karger AG, Basel
T2  - Chemotherapy
T1  - Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab
VL  - 56
IS  - 3
SP  - 214
EP  - 222
DO  - 10.1159/000316333
ER  - 
@article{
author = "Korićanac, Lela and Žakula, Jelena and Petrović, Ivan M. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra",
year = "2010",
abstract = "Background: Metastatic melanoma is one of the most aggressive tumours and is also very resistant to current therapeutic approaches. The aim of this investigation was the in vitro study of the anti-proliferative effects of fotemustine (FM; 100 and 250 mu M), bevacizumab (5 mu g/ml) and proton irradiation (12 and 16 Gy) on resistant HTB140 human melanoma cells. Methods: Viability was estimated by sulphorhodamine B assay, while cell proliferation was analyzed by 5-bromo-2-deoxyuridine assay. Cell cycle distribution and apoptosis were examined using flow cytometry. Results: Cell viability and proliferation were reduced after all applied treatments. The level of apoptosis significantly increased after treatment with FM, protons or a combination of all agents, while the apoptotic index ranged from 1.2 to 9.2. Proton irradiation, as well as combined treatment with bevacizumab and protons or 100 mu M FM, bevacizumab and protons, have reduced melanoma cell proliferation through the induction of G1 phase arrest. Single FM (250 mu M) or bevacizumab treatment and their combination, as well as the joint application of these 2 agents with protons, reduced cell proliferation and provoked G2 phase accumulation. Conclusion: The analyzed treatments reduced cell viability and proliferation, triggered G1 or G2 cell cycle phase accumulation and stimulated apoptotic cell death. Copyright (C) 2010 S. Karger AG, Basel",
journal = "Chemotherapy",
title = "Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab",
volume = "56",
number = "3",
pages = "214-222",
doi = "10.1159/000316333"
}
Korićanac, L., Žakula, J., Petrović, I. M., Valastro, L. M., Cirrone, G. A. P., Cuttone, G.,& Ristić-Fira, A.. (2010). Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab. in Chemotherapy, 56(3), 214-222.
https://doi.org/10.1159/000316333
Korićanac L, Žakula J, Petrović IM, Valastro LM, Cirrone GAP, Cuttone G, Ristić-Fira A. Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab. in Chemotherapy. 2010;56(3):214-222.
doi:10.1159/000316333 .
Korićanac, Lela, Žakula, Jelena, Petrović, Ivan M., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, "Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab" in Chemotherapy, 56, no. 3 (2010):214-222,
https://doi.org/10.1159/000316333 . .
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3

Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation

Ristić-Fira, Aleksandra; Korićanac, Lela; Žakula, Jelena; Valastro, Lucia M.; Iannolo, Gioacchin; Privitera, Giuseppe; Cuttone, Giacomo; Petrović, Ivan M.

(2009)

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Valastro, Lucia M.
AU  - Iannolo, Gioacchin
AU  - Privitera, Giuseppe
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2009
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3702
AB  - Background: Considering that HTB140 melanoma cells have shown a poor response to either protons or alkylating agents, the effects of a combined use of these agents have been analysed. Methods: Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak (SOBP). Irradiation doses were 12 or 16 Gy and are those frequently used in proton therapy. Four days after irradiation cells were treated with fotemustine (FM) or dacarbazine (DTIC). Drug concentrations were 100 and 250 mu M, values close to those that produce 50% of growth inhibition. Cell viability, proliferation, survival and cell cycle distribution were assessed 7 days after irradiation that corresponds to more than six doubling times of HTB140 cells. In this way incubation periods providing the best single effects of drugs (3 days) and protons (7 days) coincided at the same time. Results: Single proton irradiations have reduced the number of cells to similar to 50%. FM caused stronger cell inactivation due to its high toxicity, while the effectiveness of DTIC, that was important at short term, almost vanished with the incubation of 7 days. Cellular mechanisms triggered by proton irradiation differently influenced the final effects of combined treatments. Combination of protons and FM did not improve cell inactivation level achieved by single treatments. A low efficiency of the single DTIC treatment was overcome when DTIC was introduced following proton irradiation, giving better inhibitory effects with respect to the single treatments. Most of the analysed cells were in G1/S phase, viable, active and able to replicate DNA. Conclusion: The obtained results are the consequence of a high resistance of HTB140 melanoma cells to protons and/or drugs. The inactivation level of the HTB140 human melanoma cells after protons, FM or DTIC treatments was not enhanced by their combined application.
T2  - Journal of Experimental and Clinical Cancer Research
T1  - Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation
VL  - 28
DO  - 10.1186/1756-9966-28-50
ER  - 
@article{
author = "Ristić-Fira, Aleksandra and Korićanac, Lela and Žakula, Jelena and Valastro, Lucia M. and Iannolo, Gioacchin and Privitera, Giuseppe and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2009",
abstract = "Background: Considering that HTB140 melanoma cells have shown a poor response to either protons or alkylating agents, the effects of a combined use of these agents have been analysed. Methods: Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak (SOBP). Irradiation doses were 12 or 16 Gy and are those frequently used in proton therapy. Four days after irradiation cells were treated with fotemustine (FM) or dacarbazine (DTIC). Drug concentrations were 100 and 250 mu M, values close to those that produce 50% of growth inhibition. Cell viability, proliferation, survival and cell cycle distribution were assessed 7 days after irradiation that corresponds to more than six doubling times of HTB140 cells. In this way incubation periods providing the best single effects of drugs (3 days) and protons (7 days) coincided at the same time. Results: Single proton irradiations have reduced the number of cells to similar to 50%. FM caused stronger cell inactivation due to its high toxicity, while the effectiveness of DTIC, that was important at short term, almost vanished with the incubation of 7 days. Cellular mechanisms triggered by proton irradiation differently influenced the final effects of combined treatments. Combination of protons and FM did not improve cell inactivation level achieved by single treatments. A low efficiency of the single DTIC treatment was overcome when DTIC was introduced following proton irradiation, giving better inhibitory effects with respect to the single treatments. Most of the analysed cells were in G1/S phase, viable, active and able to replicate DNA. Conclusion: The obtained results are the consequence of a high resistance of HTB140 melanoma cells to protons and/or drugs. The inactivation level of the HTB140 human melanoma cells after protons, FM or DTIC treatments was not enhanced by their combined application.",
journal = "Journal of Experimental and Clinical Cancer Research",
title = "Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation",
volume = "28",
doi = "10.1186/1756-9966-28-50"
}
Ristić-Fira, A., Korićanac, L., Žakula, J., Valastro, L. M., Iannolo, G., Privitera, G., Cuttone, G.,& Petrović, I. M.. (2009). Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation. in Journal of Experimental and Clinical Cancer Research, 28.
https://doi.org/10.1186/1756-9966-28-50
Ristić-Fira A, Korićanac L, Žakula J, Valastro LM, Iannolo G, Privitera G, Cuttone G, Petrović IM. Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation. in Journal of Experimental and Clinical Cancer Research. 2009;28.
doi:10.1186/1756-9966-28-50 .
Ristić-Fira, Aleksandra, Korićanac, Lela, Žakula, Jelena, Valastro, Lucia M., Iannolo, Gioacchin, Privitera, Giuseppe, Cuttone, Giacomo, Petrović, Ivan M., "Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation" in Journal of Experimental and Clinical Cancer Research, 28 (2009),
https://doi.org/10.1186/1756-9966-28-50 . .
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