Korićanac, Lela

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Authority KeyName Variants
orcid::0000-0001-5497-284X
  • Korićanac, Lela (33)
Projects
Radiosensitivity of human genome High Energy Physics with the CMS Detector
Mechanistic studies of the reactions of transition metal ion complexes with biologically relevant molecules Ministry of Science and Technological Development of Serbia [143044, 141038], Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, Italy
Size-, shape- and structure- dependent properties of nanoparticles and nanocomposites Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, Italy
[1956] ARDITI [M1420-01-0145-FEDER-000005]
Bilateral project Republic of Serbia-People's Republic of China [451-00-478/2018-09/16] Danube Region Strategies multilateral project -Danube meets Omics [DS 052]
European Commission [2019093770] European Union REGIONAL COMPETITIVENESS ApliMetaFarma [RC.2.2.08-0046]
ENSAR - European Nuclear Science and Applications Research Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) Biomarkers in neurodegenerative and malignant processes
Preclinical investigation of bioactive substances Cellular and molecular basis of malignant and cardiovascular diseases-clinical implications
Photonics of micro and nano structured materials Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, Catania, Italy
Junta de Andalucia [UMA18-FEDERJA-126] Ministerio de Ciencia, Innovacion y Universidades [RTI2018-099668-BC22]
Ministry of Science of Serbia [143044], INFN Laboratori Nazionali del Sud, Italy National Laboratories of the South, National Institute for Nuclear Physics, Catania, Italy
Portuguese Foundation for Science and Technology [PEstOE/QUI/UI0674/2019, UID/MAT/00006/2019, INNOINDIGO/0001/2015, RAM-M1420-01-0145-FEDER-000008] Serbian-Chinese bilateral project [451-00-478/2018-09/16]
Serbian-Chinese bilateral project [SINO-SER-BIA2018002]

Author's Bibliography

SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives

Nešić, Maja D.; Dučić, Tanja; Liang, Xinyue; Algarra, Manuel; Mi, Lan; Korićanac, Lela; Žakula, Jelena; Kop, Tatjana J.; Bjelaković, Mira S.; Mitrović, Aleksandra; Gojgić Cvijović, Gordana D.; Stepić, Milutin; Petković, Marijana

(2020)

TY  - JOUR
AU  - Nešić, Maja D.
AU  - Dučić, Tanja
AU  - Liang, Xinyue
AU  - Algarra, Manuel
AU  - Mi, Lan
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Kop, Tatjana J.
AU  - Bjelaković, Mira S.
AU  - Mitrović, Aleksandra
AU  - Gojgić Cvijović, Gordana D.
AU  - Stepić, Milutin
AU  - Petković, Marijana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9712
AB  - Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide I band, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60 potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.
T2  - International Journal of Biological Macromolecules
T1  - SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives
VL  - 165
SP  - 2541
EP  - 2549
DO  - 10.1016/j.ijbiomac.2020.10.141
ER  - 
@article{
author = "Nešić, Maja D. and Dučić, Tanja and Liang, Xinyue and Algarra, Manuel and Mi, Lan and Korićanac, Lela and Žakula, Jelena and Kop, Tatjana J. and Bjelaković, Mira S. and Mitrović, Aleksandra and Gojgić Cvijović, Gordana D. and Stepić, Milutin and Petković, Marijana",
year = "2020",
abstract = "Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide I band, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60 potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.",
journal = "International Journal of Biological Macromolecules",
title = "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives",
volume = "165",
pages = "2541-2549",
doi = "10.1016/j.ijbiomac.2020.10.141"
}
Nešić, M. D., Dučić, T., Liang, X., Algarra, M., Mi, L., Korićanac, L., Žakula, J., Kop, T. J., Bjelaković, M. S., Mitrović, A., Gojgić Cvijović, G. D., Stepić, M.,& Petković, M.. (2020). SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules, 165, 2541-2549.
https://doi.org/10.1016/j.ijbiomac.2020.10.141
Nešić MD, Dučić T, Liang X, Algarra M, Mi L, Korićanac L, Žakula J, Kop TJ, Bjelaković MS, Mitrović A, Gojgić Cvijović GD, Stepić M, Petković M. SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules. 2020;165:2541-2549.
doi:10.1016/j.ijbiomac.2020.10.141 .
Nešić, Maja D., Dučić, Tanja, Liang, Xinyue, Algarra, Manuel, Mi, Lan, Korićanac, Lela, Žakula, Jelena, Kop, Tatjana J., Bjelaković, Mira S., Mitrović, Aleksandra, Gojgić Cvijović, Gordana D., Stepić, Milutin, Petković, Marijana, "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives" in International Journal of Biological Macromolecules, 165 (2020):2541-2549,
https://doi.org/10.1016/j.ijbiomac.2020.10.141 . .
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The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro

Petrović, Marija; Popović, Suzana; Baskić, Dejan; Todorović, Miloš; Đurđević, Predrag; Ristić-Fira, Aleksandra; Keta, Otilija; Petković, Vladana; Korićanac, Lela; Stojković, Danijela; Jevtić, Verica; Trifunović, Srećko; Todorović, Danijela

(2020)

TY  - JOUR
AU  - Petrović, Marija
AU  - Popović, Suzana
AU  - Baskić, Dejan
AU  - Todorović, Miloš
AU  - Đurđević, Predrag
AU  - Ristić-Fira, Aleksandra
AU  - Keta, Otilija
AU  - Petković, Vladana
AU  - Korićanac, Lela
AU  - Stojković, Danijela
AU  - Jevtić, Verica
AU  - Trifunović, Srećko
AU  - Todorović, Danijela
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9631
AB  - Aim: Newly synthesized platinum(IV) complexes with ethylenediamine-N,N’-diacetate ligands (EDDA-type) (butyl-Pt and pentyl-Pt) were investigated against two cancer (A549 lung, and HTB 140 melanoma) and one non-cancerous (MRC-5 embryonic lung fibroblast) human cell lines. Materials and Methods: The effects of these agents were compared with those of cisplatin after 6-, 24- and 48-h treatment. Sulforhodamine-B (SRB) assay was performed to estimate the cytotoxic effect, while the inhibitory effect on cell proliferation was measured using 5-bromo-2,-deoxyuridine (BrdU) incorporation assay. Cell cycle analysis was performed by flow cytometry. Type of cell death induced by these agents was determined by electrophoretic analysis of DNA, flow cytometry and by western blot analysis of proteins involved in induction of apoptosis. The effects of gamma irradiation, alone and in combination with platinum-based compounds, were examined by clonogenic and SRB assays. Results: All examined platinum-based compounds had inhibitory and antiproliferative effects on A549 cells, but not on HTB140 and MRC-5 cells. Butyl-Pt, pentyl-Pt and cisplatin arrested the cell cycle in the S-phase and induced apoptotic cell death via regulation of expression of B-cell lymphoma 2 (BCL2) and BCL2-associated X (BAX) proteins. Platinum-based compounds increased the sensitivity of A549 cells to gamma irradiation. Butyl-Pt and pentyl-Pt showed better antitumour effects against A549 cells than did cisplatin, by interfering in cell proliferation and the cell cycle, and by triggering apoptosis. Conclusion: The effects of gamma irradiation on tumour cells may be amplified by pre-treatment of cells with platinum-based compounds.
T2  - Anticancer Research
T1  - The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro
VL  - 40
IS  - 9
SP  - 5001
EP  - 5013
DO  - 10.21873/anticanres.14503
ER  - 
@article{
author = "Petrović, Marija and Popović, Suzana and Baskić, Dejan and Todorović, Miloš and Đurđević, Predrag and Ristić-Fira, Aleksandra and Keta, Otilija and Petković, Vladana and Korićanac, Lela and Stojković, Danijela and Jevtić, Verica and Trifunović, Srećko and Todorović, Danijela",
year = "2020",
abstract = "Aim: Newly synthesized platinum(IV) complexes with ethylenediamine-N,N’-diacetate ligands (EDDA-type) (butyl-Pt and pentyl-Pt) were investigated against two cancer (A549 lung, and HTB 140 melanoma) and one non-cancerous (MRC-5 embryonic lung fibroblast) human cell lines. Materials and Methods: The effects of these agents were compared with those of cisplatin after 6-, 24- and 48-h treatment. Sulforhodamine-B (SRB) assay was performed to estimate the cytotoxic effect, while the inhibitory effect on cell proliferation was measured using 5-bromo-2,-deoxyuridine (BrdU) incorporation assay. Cell cycle analysis was performed by flow cytometry. Type of cell death induced by these agents was determined by electrophoretic analysis of DNA, flow cytometry and by western blot analysis of proteins involved in induction of apoptosis. The effects of gamma irradiation, alone and in combination with platinum-based compounds, were examined by clonogenic and SRB assays. Results: All examined platinum-based compounds had inhibitory and antiproliferative effects on A549 cells, but not on HTB140 and MRC-5 cells. Butyl-Pt, pentyl-Pt and cisplatin arrested the cell cycle in the S-phase and induced apoptotic cell death via regulation of expression of B-cell lymphoma 2 (BCL2) and BCL2-associated X (BAX) proteins. Platinum-based compounds increased the sensitivity of A549 cells to gamma irradiation. Butyl-Pt and pentyl-Pt showed better antitumour effects against A549 cells than did cisplatin, by interfering in cell proliferation and the cell cycle, and by triggering apoptosis. Conclusion: The effects of gamma irradiation on tumour cells may be amplified by pre-treatment of cells with platinum-based compounds.",
journal = "Anticancer Research",
title = "The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro",
volume = "40",
number = "9",
pages = "5001-5013",
doi = "10.21873/anticanres.14503"
}
Petrović, M., Popović, S., Baskić, D., Todorović, M., Đurđević, P., Ristić-Fira, A., Keta, O., Petković, V., Korićanac, L., Stojković, D., Jevtić, V., Trifunović, S.,& Todorović, D.. (2020). The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro. in Anticancer Research, 40(9), 5001-5013.
https://doi.org/10.21873/anticanres.14503
Petrović M, Popović S, Baskić D, Todorović M, Đurđević P, Ristić-Fira A, Keta O, Petković V, Korićanac L, Stojković D, Jevtić V, Trifunović S, Todorović D. The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro. in Anticancer Research. 2020;40(9):5001-5013.
doi:10.21873/anticanres.14503 .
Petrović, Marija, Popović, Suzana, Baskić, Dejan, Todorović, Miloš, Đurđević, Predrag, Ristić-Fira, Aleksandra, Keta, Otilija, Petković, Vladana, Korićanac, Lela, Stojković, Danijela, Jevtić, Verica, Trifunović, Srećko, Todorović, Danijela, "The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro" in Anticancer Research, 40, no. 9 (2020):5001-5013,
https://doi.org/10.21873/anticanres.14503 . .
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1

Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer

Matijević, Milica; Nakarada, Đura; Liang, Xinyue; Korićanac, Lela; Rajsiglova, Lenka; Vannucci, Luca; Nešić, Maja D.; Vranješ, Mila; Mojović, Miloš D.; Mi, Lan; Estrela-Lopis, Irina; Böttner, Julia; Šaponjić, Zoran; Petković, Marijana; Stepić, Milutin

(2020)

TY  - JOUR
AU  - Matijević, Milica
AU  - Nakarada, Đura
AU  - Liang, Xinyue
AU  - Korićanac, Lela
AU  - Rajsiglova, Lenka
AU  - Vannucci, Luca
AU  - Nešić, Maja D.
AU  - Vranješ, Mila
AU  - Mojović, Miloš D.
AU  - Mi, Lan
AU  - Estrela-Lopis, Irina
AU  - Böttner, Julia
AU  - Šaponjić, Zoran
AU  - Petković, Marijana
AU  - Stepić, Milutin
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9058
AB  - TiO2 prolatenanospheroids (PNSs) may be photosensitizers (PSs), which act by catalyzation of hydroxyl radical (∙OH) formation upon light illumination. ∙OH might, in turn, contribute to killing of cancer cells. On the other hand, there is great concern about toxicity in the dark of TiO2 nanoparticles in general. In this work, we have investigated the biocompatibility of TiO2 PNSs of the anatase crystal form (length between 100 and 300 nm and width 50 nm) in the dark with immune cells and light-induced cytotoxicity on several cancer cell lines. The effects of the treatment of different cell lines with several concentrations of TiO2 PNSs suspensions showed the specifics of cells’ viability and the intracellular localization. The results of in vitro studies obtained by cytotoxicity assays adjusted to individual cell lines’ metabolism point towards the biocompatibility of TiO2 PNSs at low and moderate concentrations in the dark, which neither kill the cells, nor induce activation of the immune system cells. Laser scanning confocal microscopy revealed that PNSs are taken up by cells, and insight into the intracellular distribution was obtained in this study.
T2  - Journal of Nanoparticle Research
T1  - Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer
VL  - 22
IS  - 7
SP  - 175
DO  - 10.1007/s11051-020-04899-3
ER  - 
@article{
author = "Matijević, Milica and Nakarada, Đura and Liang, Xinyue and Korićanac, Lela and Rajsiglova, Lenka and Vannucci, Luca and Nešić, Maja D. and Vranješ, Mila and Mojović, Miloš D. and Mi, Lan and Estrela-Lopis, Irina and Böttner, Julia and Šaponjić, Zoran and Petković, Marijana and Stepić, Milutin",
year = "2020",
abstract = "TiO2 prolatenanospheroids (PNSs) may be photosensitizers (PSs), which act by catalyzation of hydroxyl radical (∙OH) formation upon light illumination. ∙OH might, in turn, contribute to killing of cancer cells. On the other hand, there is great concern about toxicity in the dark of TiO2 nanoparticles in general. In this work, we have investigated the biocompatibility of TiO2 PNSs of the anatase crystal form (length between 100 and 300 nm and width 50 nm) in the dark with immune cells and light-induced cytotoxicity on several cancer cell lines. The effects of the treatment of different cell lines with several concentrations of TiO2 PNSs suspensions showed the specifics of cells’ viability and the intracellular localization. The results of in vitro studies obtained by cytotoxicity assays adjusted to individual cell lines’ metabolism point towards the biocompatibility of TiO2 PNSs at low and moderate concentrations in the dark, which neither kill the cells, nor induce activation of the immune system cells. Laser scanning confocal microscopy revealed that PNSs are taken up by cells, and insight into the intracellular distribution was obtained in this study.",
journal = "Journal of Nanoparticle Research",
title = "Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer",
volume = "22",
number = "7",
pages = "175",
doi = "10.1007/s11051-020-04899-3"
}
Matijević, M., Nakarada, Đ., Liang, X., Korićanac, L., Rajsiglova, L., Vannucci, L., Nešić, M. D., Vranješ, M., Mojović, M. D., Mi, L., Estrela-Lopis, I., Böttner, J., Šaponjić, Z., Petković, M.,& Stepić, M.. (2020). Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer. in Journal of Nanoparticle Research, 22(7), 175.
https://doi.org/10.1007/s11051-020-04899-3
Matijević M, Nakarada Đ, Liang X, Korićanac L, Rajsiglova L, Vannucci L, Nešić MD, Vranješ M, Mojović MD, Mi L, Estrela-Lopis I, Böttner J, Šaponjić Z, Petković M, Stepić M. Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer. in Journal of Nanoparticle Research. 2020;22(7):175.
doi:10.1007/s11051-020-04899-3 .
Matijević, Milica, Nakarada, Đura, Liang, Xinyue, Korićanac, Lela, Rajsiglova, Lenka, Vannucci, Luca, Nešić, Maja D., Vranješ, Mila, Mojović, Miloš D., Mi, Lan, Estrela-Lopis, Irina, Böttner, Julia, Šaponjić, Zoran, Petković, Marijana, Stepić, Milutin, "Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer" in Journal of Nanoparticle Research, 22, no. 7 (2020):175,
https://doi.org/10.1007/s11051-020-04899-3 . .
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1

Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex

Nešić, Maja A.; Žakula, Jelena; Korićanac, Lela; Stepić, Milutin; Radoičić, Marija B.; Popović, Iva A.; Šaponjić, Zoran; Petković, Marijana

(2017)

TY  - JOUR
AU  - Nešić, Maja A.
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Stepić, Milutin
AU  - Radoičić, Marija B.
AU  - Popović, Iva A.
AU  - Šaponjić, Zoran
AU  - Petković, Marijana
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1747
AB  - We studied the colloidal TiO2 nanoparticles as a carrier for controlled delivery of the ruthenium complex to the melanoma cell line. The system demonstrated slower complex release upon visible and increased release rate upon UV light illumination. Accordingly, the light-dependent cytotoxicity of the system was demonstrated on amelanotic melanoma cancer line. The cell death is enhanced by UV and reduced by red light in the presence of investigated nanocomposite system. Both components of the system may act as photosensitizers, by generating reactive oxygen species, which promote cell death. Thus, the system might act dually, as photodynamic therapeutic agent and as the light tunable system for metallo-drug delivery and it might be of interest for development of new more efficient drug delivery approaches by using a light as external stimulus. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Journal of Photochemistry and Photobiology. A: Chemistry
T1  - Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex
VL  - 347
SP  - 55
EP  - 66
DO  - 10.1016/jjphotochem.2017.06.045
ER  - 
@article{
author = "Nešić, Maja A. and Žakula, Jelena and Korićanac, Lela and Stepić, Milutin and Radoičić, Marija B. and Popović, Iva A. and Šaponjić, Zoran and Petković, Marijana",
year = "2017",
abstract = "We studied the colloidal TiO2 nanoparticles as a carrier for controlled delivery of the ruthenium complex to the melanoma cell line. The system demonstrated slower complex release upon visible and increased release rate upon UV light illumination. Accordingly, the light-dependent cytotoxicity of the system was demonstrated on amelanotic melanoma cancer line. The cell death is enhanced by UV and reduced by red light in the presence of investigated nanocomposite system. Both components of the system may act as photosensitizers, by generating reactive oxygen species, which promote cell death. Thus, the system might act dually, as photodynamic therapeutic agent and as the light tunable system for metallo-drug delivery and it might be of interest for development of new more efficient drug delivery approaches by using a light as external stimulus. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Journal of Photochemistry and Photobiology. A: Chemistry",
title = "Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex",
volume = "347",
pages = "55-66",
doi = "10.1016/jjphotochem.2017.06.045"
}
Nešić, M. A., Žakula, J., Korićanac, L., Stepić, M., Radoičić, M. B., Popović, I. A., Šaponjić, Z.,& Petković, M.. (2017). Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex. in Journal of Photochemistry and Photobiology. A: Chemistry, 347, 55-66.
https://doi.org/10.1016/jjphotochem.2017.06.045
Nešić MA, Žakula J, Korićanac L, Stepić M, Radoičić MB, Popović IA, Šaponjić Z, Petković M. Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex. in Journal of Photochemistry and Photobiology. A: Chemistry. 2017;347:55-66.
doi:10.1016/jjphotochem.2017.06.045 .
Nešić, Maja A., Žakula, Jelena, Korićanac, Lela, Stepić, Milutin, Radoičić, Marija B., Popović, Iva A., Šaponjić, Zoran, Petković, Marijana, "Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex" in Journal of Photochemistry and Photobiology. A: Chemistry, 347 (2017):55-66,
https://doi.org/10.1016/jjphotochem.2017.06.045 . .
9

Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells

Žakula, Jelena; Korićanac, Lela; Keta, Otilija D.; Todorović, Danijela V.; Cirrone, Giuseppe Antonio Pablo; Romano, Francesco; Cuttone, Giacomo; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(2016)

TY  - JOUR
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1290
AB  - Background and objectives: The main goal when treating malignancies with radiation is to deprive tumour cells of their reproductive potential. One approach is to induce tumour cell apoptosis. This study was conducted to evaluate the ability of carbon ions (C-12) to induce apoptosis and cell cycle arrest in human HTB140 melanoma cells. Methods: In this in vitro study, human melanoma HTB140 cells were irradiated with the 62 MeV/n carbon (C-12) ion beam, having two different linear energy transfer (LET) values: 197 and 382 keV/mu m. The dose range was 2 to 16 Gy. Cell viability was estimated by the sulforhodamine B assay seven days after irradiation. The cell cycle and apoptosis were evaluated 48 h after irradiation using flow cytometry. At the same time point, protein and gene expression of apoptotic regulators were estimated using the Western blot and q-PCR methods, respectively. Results: Cell viability experiments indicated strong anti-tumour effects of C-12 ions. The analysis of cell cycle showed that C-12 ions blocked HTB140 cells in G2 phase and induced the dose dependent increase of apoptosis. The maximum value of 21.8 per cent was attained after irradiation with LET of 197 keV/mu m at the dose level of 16 Gy. Pro-apoptotic effects of C-12 ions were confirmed by changes of key apoptotic molecules: the p53, Bax, Bcl-2, poly ADP ribose polymerase (PARP) as well as nuclear factor kappa B (NF kappa B). At the level of protein expression, the results indicated significant increases of p53, NF kappa B and Bax/Bcl-2 ratio and PARP cleavage. The Bax/Bcl-2 mRNA ratio was also increased, while no change was detected in the level of NF kappa B mRNA. Interpretation and conclusions: The present results indicated that anti-tumour effects of C-12 ions in human melanoma HTB140 cells were accomplished through induction of the mitochondrial apoptotic pathway as well as G2 arrest.
T2  - Indian Journal of Medical Research
T1  - Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells
VL  - 143
SP  - 120
EP  - 128
DO  - 10.4103/0971-5916.191811
ER  - 
@article{
author = "Žakula, Jelena and Korićanac, Lela and Keta, Otilija D. and Todorović, Danijela V. and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2016",
abstract = "Background and objectives: The main goal when treating malignancies with radiation is to deprive tumour cells of their reproductive potential. One approach is to induce tumour cell apoptosis. This study was conducted to evaluate the ability of carbon ions (C-12) to induce apoptosis and cell cycle arrest in human HTB140 melanoma cells. Methods: In this in vitro study, human melanoma HTB140 cells were irradiated with the 62 MeV/n carbon (C-12) ion beam, having two different linear energy transfer (LET) values: 197 and 382 keV/mu m. The dose range was 2 to 16 Gy. Cell viability was estimated by the sulforhodamine B assay seven days after irradiation. The cell cycle and apoptosis were evaluated 48 h after irradiation using flow cytometry. At the same time point, protein and gene expression of apoptotic regulators were estimated using the Western blot and q-PCR methods, respectively. Results: Cell viability experiments indicated strong anti-tumour effects of C-12 ions. The analysis of cell cycle showed that C-12 ions blocked HTB140 cells in G2 phase and induced the dose dependent increase of apoptosis. The maximum value of 21.8 per cent was attained after irradiation with LET of 197 keV/mu m at the dose level of 16 Gy. Pro-apoptotic effects of C-12 ions were confirmed by changes of key apoptotic molecules: the p53, Bax, Bcl-2, poly ADP ribose polymerase (PARP) as well as nuclear factor kappa B (NF kappa B). At the level of protein expression, the results indicated significant increases of p53, NF kappa B and Bax/Bcl-2 ratio and PARP cleavage. The Bax/Bcl-2 mRNA ratio was also increased, while no change was detected in the level of NF kappa B mRNA. Interpretation and conclusions: The present results indicated that anti-tumour effects of C-12 ions in human melanoma HTB140 cells were accomplished through induction of the mitochondrial apoptotic pathway as well as G2 arrest.",
journal = "Indian Journal of Medical Research",
title = "Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells",
volume = "143",
pages = "120-128",
doi = "10.4103/0971-5916.191811"
}
Žakula, J., Korićanac, L., Keta, O. D., Todorović, D. V., Cirrone, G. A. P., Romano, F., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2016). Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells. in Indian Journal of Medical Research, 143, 120-128.
https://doi.org/10.4103/0971-5916.191811
Žakula J, Korićanac L, Keta OD, Todorović DV, Cirrone GAP, Romano F, Cuttone G, Petrović IM, Ristić-Fira A. Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells. in Indian Journal of Medical Research. 2016;143:120-128.
doi:10.4103/0971-5916.191811 .
Žakula, Jelena, Korićanac, Lela, Keta, Otilija D., Todorović, Danijela V., Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells" in Indian Journal of Medical Research, 143 (2016):120-128,
https://doi.org/10.4103/0971-5916.191811 . .
3
1
2

Radiation dose determines the method for quantification of DNA double strand breaks

Bulat, Tanja M.; Keta, Otilija D.; Korićanac, Lela; Žakula, Jelena; Petrović, Ivan M.; Ristić-Fira, Aleksandra; Todorović, Danijela V.

(2016)

TY  - JOUR
AU  - Bulat, Tanja M.
AU  - Keta, Otilija D.
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/970
AB  - Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (gamma H2AX). Immunofluorescent staining visualizes formation of gamma H2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position and intensity of fluorescent signal in each single cell. In practice there are problems in quantification of gamma H2AX. This paper is based on two issues: the determination of which technique should be applied concerning the radiation dose, and how to analyze fluorescent microscopy images obtained by different microscopes. HTB140 melanoma cells were exposed to gamma-rays, in the dose range from 1 to 16 Gy. Radiation effects on the DNA level were analyzed at different time intervals after irradiation by Western blot analysis and immunofluorescence microscopy. Immunochemically stained cells were visualized with two types of microscopes: AxioVision (Zeiss, Germany) microscope, comprising an ApoTome software, and AxioImagerA1 microscope (Zeiss, Germany). Obtained results show that the level of gamma H2AX is time and dose dependent. Immunofluorescence microscopy provided better detection of DSBs for lower irradiation doses, while Western blot analysis was more reliable for higher irradiation doses. AxioVision microscope containing ApoTome software was more suitable for the detection of gamma H2AX foci.
T2  - Anais de Academia Brasileira de Ciencias
T1  - Radiation dose determines the method for quantification of DNA double strand breaks
VL  - 88
IS  - 1
SP  - 127
EP  - 136
DO  - 10.1590/0001-3765201620140553
ER  - 
@article{
author = "Bulat, Tanja M. and Keta, Otilija D. and Korićanac, Lela and Žakula, Jelena and Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V.",
year = "2016",
abstract = "Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (gamma H2AX). Immunofluorescent staining visualizes formation of gamma H2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position and intensity of fluorescent signal in each single cell. In practice there are problems in quantification of gamma H2AX. This paper is based on two issues: the determination of which technique should be applied concerning the radiation dose, and how to analyze fluorescent microscopy images obtained by different microscopes. HTB140 melanoma cells were exposed to gamma-rays, in the dose range from 1 to 16 Gy. Radiation effects on the DNA level were analyzed at different time intervals after irradiation by Western blot analysis and immunofluorescence microscopy. Immunochemically stained cells were visualized with two types of microscopes: AxioVision (Zeiss, Germany) microscope, comprising an ApoTome software, and AxioImagerA1 microscope (Zeiss, Germany). Obtained results show that the level of gamma H2AX is time and dose dependent. Immunofluorescence microscopy provided better detection of DSBs for lower irradiation doses, while Western blot analysis was more reliable for higher irradiation doses. AxioVision microscope containing ApoTome software was more suitable for the detection of gamma H2AX foci.",
journal = "Anais de Academia Brasileira de Ciencias",
title = "Radiation dose determines the method for quantification of DNA double strand breaks",
volume = "88",
number = "1",
pages = "127-136",
doi = "10.1590/0001-3765201620140553"
}
Bulat, T. M., Keta, O. D., Korićanac, L., Žakula, J., Petrović, I. M., Ristić-Fira, A.,& Todorović, D. V.. (2016). Radiation dose determines the method for quantification of DNA double strand breaks. in Anais de Academia Brasileira de Ciencias, 88(1), 127-136.
https://doi.org/10.1590/0001-3765201620140553
Bulat TM, Keta OD, Korićanac L, Žakula J, Petrović IM, Ristić-Fira A, Todorović DV. Radiation dose determines the method for quantification of DNA double strand breaks. in Anais de Academia Brasileira de Ciencias. 2016;88(1):127-136.
doi:10.1590/0001-3765201620140553 .
Bulat, Tanja M., Keta, Otilija D., Korićanac, Lela, Žakula, Jelena, Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., "Radiation dose determines the method for quantification of DNA double strand breaks" in Anais de Academia Brasileira de Ciencias, 88, no. 1 (2016):127-136,
https://doi.org/10.1590/0001-3765201620140553 . .
7
4
6

Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin

Nišavić, Marija; Masnikosa, Romana; Butorac, Ana; Perica, Kristina; Rilak, Ana; Korićanac, Lela; Hozic, Amela; Petković, Marijana; Cindrić, Mario

(2016)

TY  - JOUR
AU  - Nišavić, Marija
AU  - Masnikosa, Romana
AU  - Butorac, Ana
AU  - Perica, Kristina
AU  - Rilak, Ana
AU  - Korićanac, Lela
AU  - Hozic, Amela
AU  - Petković, Marijana
AU  - Cindrić, Mario
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1124
AB  - Hyphenated mass spectrometry (MS) techniques have attained an important position in analysis of covalent and non-covalent interactions of metal complexes with peptides and proteins. The aim of the present study was to qualitatively and quantitatively determine ruthenium binding sites on a protein using tandem mass spectrometry and allied techniques, i.e. liquid chromatography (LC) and inductively coupled plasma optical emission spectrometry (ICP-OES). For that purpose, two newly synthesized Ru(II) complexes of a meridional geometry, namely mer-[Ru(4 Cl-tpy)(en)Cl](+) (1) and mer-[Ru(4 Cl-tpy)(dach)Cl](+) (2) (where 4 Cl-tpy = 4-chloro-2,2:6,2 -terpyridine, en = 1,2-diaminoethane and dach = 1,2-diaminocyclohexane), and bovine serum albumin were used. The binding of the complexes to the protein was investigated by means of size exclusion- and reversed phase-LC, ICP OES, matrix-assisted laser desorption ionization MS and MS/MS. Ruthenated peptide sequence and a binding target amino acid were revealed through accurate elucidation of MS/MS spectra. The results obtained in this study suggest a high binding capacity of the protein towards both complexes, with up to 5.77 +/- 0.14 and 6.95 +/- 0.43 mol of 1 and 2 bound per mol of protein, respectively. The proposed binding mechanism for the selected complexes includes the release of Cl ligand, its replacement with water molecule and further coordination to electron donor histidine residue. (C) 2016 Elsevier Inc. All rights reserved.
T2  - Journal of Inorganic Biochemistry
T1  - Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin
VL  - 159
SP  - 89
EP  - 95
DO  - 10.1016/j.jinorgbio.2016.02.034
ER  - 
@article{
author = "Nišavić, Marija and Masnikosa, Romana and Butorac, Ana and Perica, Kristina and Rilak, Ana and Korićanac, Lela and Hozic, Amela and Petković, Marijana and Cindrić, Mario",
year = "2016",
abstract = "Hyphenated mass spectrometry (MS) techniques have attained an important position in analysis of covalent and non-covalent interactions of metal complexes with peptides and proteins. The aim of the present study was to qualitatively and quantitatively determine ruthenium binding sites on a protein using tandem mass spectrometry and allied techniques, i.e. liquid chromatography (LC) and inductively coupled plasma optical emission spectrometry (ICP-OES). For that purpose, two newly synthesized Ru(II) complexes of a meridional geometry, namely mer-[Ru(4 Cl-tpy)(en)Cl](+) (1) and mer-[Ru(4 Cl-tpy)(dach)Cl](+) (2) (where 4 Cl-tpy = 4-chloro-2,2:6,2 -terpyridine, en = 1,2-diaminoethane and dach = 1,2-diaminocyclohexane), and bovine serum albumin were used. The binding of the complexes to the protein was investigated by means of size exclusion- and reversed phase-LC, ICP OES, matrix-assisted laser desorption ionization MS and MS/MS. Ruthenated peptide sequence and a binding target amino acid were revealed through accurate elucidation of MS/MS spectra. The results obtained in this study suggest a high binding capacity of the protein towards both complexes, with up to 5.77 +/- 0.14 and 6.95 +/- 0.43 mol of 1 and 2 bound per mol of protein, respectively. The proposed binding mechanism for the selected complexes includes the release of Cl ligand, its replacement with water molecule and further coordination to electron donor histidine residue. (C) 2016 Elsevier Inc. All rights reserved.",
journal = "Journal of Inorganic Biochemistry",
title = "Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin",
volume = "159",
pages = "89-95",
doi = "10.1016/j.jinorgbio.2016.02.034"
}
Nišavić, M., Masnikosa, R., Butorac, A., Perica, K., Rilak, A., Korićanac, L., Hozic, A., Petković, M.,& Cindrić, M.. (2016). Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin. in Journal of Inorganic Biochemistry, 159, 89-95.
https://doi.org/10.1016/j.jinorgbio.2016.02.034
Nišavić M, Masnikosa R, Butorac A, Perica K, Rilak A, Korićanac L, Hozic A, Petković M, Cindrić M. Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin. in Journal of Inorganic Biochemistry. 2016;159:89-95.
doi:10.1016/j.jinorgbio.2016.02.034 .
Nišavić, Marija, Masnikosa, Romana, Butorac, Ana, Perica, Kristina, Rilak, Ana, Korićanac, Lela, Hozic, Amela, Petković, Marijana, Cindrić, Mario, "Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin" in Journal of Inorganic Biochemistry, 159 (2016):89-95,
https://doi.org/10.1016/j.jinorgbio.2016.02.034 . .
11
10
12

Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons

Keta, Otilija D.; Todorović, Danijela V.; Popović, Nataša M.; Korićanac, Lela; Cuttone, Giacomo; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(2014)

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Popović, Nataša M.
AU  - Korićanac, Lela
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5447
AB  - Introduction: Proton radiation offers physical advantages over conventional radiation. Radiosensitivity of human 59M ovarian cancer and HTB140 melanoma cells was investigated after exposure to gamma-rays and protons. Material and methods: Irradiations were performed in the middle of a 62 MeV therapeutic proton spread out Bragg peak with doses ranging from 2 to 16 Gy. The mean energy of protons was 34.88+/-2.15 MeV, corresponding to the linear energy transfer of 4.7+/-0.2 keV/mu m. Irradiations with gamma-rays were performed using the same doses. Viability, proliferation and survival were assessed 7 days after both types of irradiation while analyses of cell cycle and apoptosis were performed 48 h after irradiation. Results: Results showed that gamma-rays and protons reduced the number of viable cells for both cell lines, with stronger inactivation achieved after irradiation with protons. Surviving fractions for 59M were 0.91+/-0.01 for gamma-rays and 0.81+/-0.01 for protons, while those for HTB140 cells were 0.93+/-0.01 for gamma-rays and 0.86+/-0.01 for protons. Relative biological effectiveness of protons, being 2.47+/-0.22 for 59M and 2.08+/-0.36 for HTB140, indicated that protons provoked better cell elimination than gamma-rays. After proton irradiation proliferation capacity of the two cell lines was slightly higher as compared to gamma-rays. Proliferation was higher for 59M than for HTB140 cells after both types of irradiation. Induction of apoptosis and G2 arrest detected after proton irradiation were more prominent in 59M cells. Conclusions: The obtained results suggest that protons exert better antitumour effects on ovarian carcinoma and melanoma cells than gamma-rays. The dissimilar response of these cells to radiation is related to their different features.
T2  - Archives of Medical Science
T1  - Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons
VL  - 10
IS  - 3
SP  - 578
EP  - 586
DO  - 10.5114/aoms.2014.43751
ER  - 
@article{
author = "Keta, Otilija D. and Todorović, Danijela V. and Popović, Nataša M. and Korićanac, Lela and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2014",
abstract = "Introduction: Proton radiation offers physical advantages over conventional radiation. Radiosensitivity of human 59M ovarian cancer and HTB140 melanoma cells was investigated after exposure to gamma-rays and protons. Material and methods: Irradiations were performed in the middle of a 62 MeV therapeutic proton spread out Bragg peak with doses ranging from 2 to 16 Gy. The mean energy of protons was 34.88+/-2.15 MeV, corresponding to the linear energy transfer of 4.7+/-0.2 keV/mu m. Irradiations with gamma-rays were performed using the same doses. Viability, proliferation and survival were assessed 7 days after both types of irradiation while analyses of cell cycle and apoptosis were performed 48 h after irradiation. Results: Results showed that gamma-rays and protons reduced the number of viable cells for both cell lines, with stronger inactivation achieved after irradiation with protons. Surviving fractions for 59M were 0.91+/-0.01 for gamma-rays and 0.81+/-0.01 for protons, while those for HTB140 cells were 0.93+/-0.01 for gamma-rays and 0.86+/-0.01 for protons. Relative biological effectiveness of protons, being 2.47+/-0.22 for 59M and 2.08+/-0.36 for HTB140, indicated that protons provoked better cell elimination than gamma-rays. After proton irradiation proliferation capacity of the two cell lines was slightly higher as compared to gamma-rays. Proliferation was higher for 59M than for HTB140 cells after both types of irradiation. Induction of apoptosis and G2 arrest detected after proton irradiation were more prominent in 59M cells. Conclusions: The obtained results suggest that protons exert better antitumour effects on ovarian carcinoma and melanoma cells than gamma-rays. The dissimilar response of these cells to radiation is related to their different features.",
journal = "Archives of Medical Science",
title = "Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons",
volume = "10",
number = "3",
pages = "578-586",
doi = "10.5114/aoms.2014.43751"
}
Keta, O. D., Todorović, D. V., Popović, N. M., Korićanac, L., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2014). Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons. in Archives of Medical Science, 10(3), 578-586.
https://doi.org/10.5114/aoms.2014.43751
Keta OD, Todorović DV, Popović NM, Korićanac L, Cuttone G, Petrović IM, Ristić-Fira A. Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons. in Archives of Medical Science. 2014;10(3):578-586.
doi:10.5114/aoms.2014.43751 .
Keta, Otilija D., Todorović, Danijela V., Popović, Nataša M., Korićanac, Lela, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons" in Archives of Medical Science, 10, no. 3 (2014):578-586,
https://doi.org/10.5114/aoms.2014.43751 . .
8
8
10

Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition

Keta, Otilija D.; Bulat, Tanja M.; Korićanac, Lela; Žakula, Jelena; Cuttone, Giacomo; Privitera, Giuseppe; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(2014)

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Bulat, Tanja M.
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Cuttone, Giacomo
AU  - Privitera, Giuseppe
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/167
AB  - Molecular targeted cancer therapy is a promising treatment strategy. Considering the central role of the epidermal growth factor receptor in cell proliferation and survival, there are indications that targeted agents like tyrosine kinase inhibitors, i. e., erlotinib, may enhance the antitumor treatment by radiation. The aim of this study is to analyze the inactivation effects of gamma-rays and to test the radiosensitizing potential of erlotinib on human lung adenocarcinoma cells in vitro. Irradiations were performed with doses ranging from 1 Gy to 8 Gy. In order to increase the radiosensitivity of CRL-5876 lung adenocarcinoma cells, the cells were treated with a clinically relevant concentration of 2 mu M erlotinib. The effects of single and combined treatments were monitored using clonogenic survival, cell viability and proliferation assays at different time points. For the detection and visualization of the phosphorylated histone H2AX (gamma-H2AX), an important biological marker of DNA double-strand break formation, fluorescence inununocytochemistry, was performed. The response to the treatment was monitored at four time points: 30 min, 2, 6, and 24 h. Irradiations with gamma-rays resulted in significant cell inactivation regarding all analyzed biological endpoints. Combined treatments revealed consistent cell inactivation. Moreover, compared to gamma-rays alone, elevated levels of gamma-H2AX foci were observed after pretreatment with erlotinib, indicating radiosensitization through impaired DNA repair.
T2  - Nuclear technology and radiation protection
T1  - Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition
VL  - 29
IS  - 3
SP  - 233
EP  - 241
DO  - 10.2298/NTRP1403233K
ER  - 
@article{
author = "Keta, Otilija D. and Bulat, Tanja M. and Korićanac, Lela and Žakula, Jelena and Cuttone, Giacomo and Privitera, Giuseppe and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2014",
abstract = "Molecular targeted cancer therapy is a promising treatment strategy. Considering the central role of the epidermal growth factor receptor in cell proliferation and survival, there are indications that targeted agents like tyrosine kinase inhibitors, i. e., erlotinib, may enhance the antitumor treatment by radiation. The aim of this study is to analyze the inactivation effects of gamma-rays and to test the radiosensitizing potential of erlotinib on human lung adenocarcinoma cells in vitro. Irradiations were performed with doses ranging from 1 Gy to 8 Gy. In order to increase the radiosensitivity of CRL-5876 lung adenocarcinoma cells, the cells were treated with a clinically relevant concentration of 2 mu M erlotinib. The effects of single and combined treatments were monitored using clonogenic survival, cell viability and proliferation assays at different time points. For the detection and visualization of the phosphorylated histone H2AX (gamma-H2AX), an important biological marker of DNA double-strand break formation, fluorescence inununocytochemistry, was performed. The response to the treatment was monitored at four time points: 30 min, 2, 6, and 24 h. Irradiations with gamma-rays resulted in significant cell inactivation regarding all analyzed biological endpoints. Combined treatments revealed consistent cell inactivation. Moreover, compared to gamma-rays alone, elevated levels of gamma-H2AX foci were observed after pretreatment with erlotinib, indicating radiosensitization through impaired DNA repair.",
journal = "Nuclear technology and radiation protection",
title = "Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition",
volume = "29",
number = "3",
pages = "233-241",
doi = "10.2298/NTRP1403233K"
}
Keta, O. D., Bulat, T. M., Korićanac, L., Žakula, J., Cuttone, G., Privitera, G., Petrović, I. M.,& Ristić-Fira, A.. (2014). Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition. in Nuclear technology and radiation protection, 29(3), 233-241.
https://doi.org/10.2298/NTRP1403233K
Keta OD, Bulat TM, Korićanac L, Žakula J, Cuttone G, Privitera G, Petrović IM, Ristić-Fira A. Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition. in Nuclear technology and radiation protection. 2014;29(3):233-241.
doi:10.2298/NTRP1403233K .
Keta, Otilija D., Bulat, Tanja M., Korićanac, Lela, Žakula, Jelena, Cuttone, Giacomo, Privitera, Giuseppe, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Radiosensitization of Non-Small Cell Lung Carcinoma By Egfr Inhibition" in Nuclear technology and radiation protection, 29, no. 3 (2014):233-241,
https://doi.org/10.2298/NTRP1403233K . .
2
2
2

Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells

Korićanac, Lela; Žakula, Jelena; Keta, Otilija D.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Ristić-Fira, Aleksandra; Petrović, Ivan M.

(2013)

TY  - JOUR
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Keta, Otilija D.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5593
AB  - This study was conducted in order to evaluate the ability of carbon ions to induce DNA double-strand breaks and apoptosis in the radio-resistant human HTB140 melanoma cells. The cells were irradiated with C-12 ions having the linear energy transfer of 258 keV/mu m. Irradiations were performed in the dose range from 2 to 16 Gy. Induction of DNA double-strand breaks was evaluated 2 hour after irradiation through expression of gamma H2AX protein. Increased level of gamma H2AX detected in irradiated samples was especially high after irradiation with 12 and 16 Gy. Dose dependent increase of apoptosis was detected 48 hour after irradiation by flow-cytometry, with the maximum value of 20.4% after irradiation with 16 Gy, and the apoptotic index of 9.3. Pro-apoptotic effects of carbon ion beams were confirmed by changes of key molecules of the mitochondrial apoptotic pathway, p53 protein expression, Bax/Bcl-2 ratio and caspase-3 activation.
T2  - Nuclear technology and radiation protection
T1  - Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells
VL  - 28
IS  - 2
SP  - 195
EP  - 203
DO  - 10.2298/NTRP1302195K
ER  - 
@article{
author = "Korićanac, Lela and Žakula, Jelena and Keta, Otilija D. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra and Petrović, Ivan M.",
year = "2013",
abstract = "This study was conducted in order to evaluate the ability of carbon ions to induce DNA double-strand breaks and apoptosis in the radio-resistant human HTB140 melanoma cells. The cells were irradiated with C-12 ions having the linear energy transfer of 258 keV/mu m. Irradiations were performed in the dose range from 2 to 16 Gy. Induction of DNA double-strand breaks was evaluated 2 hour after irradiation through expression of gamma H2AX protein. Increased level of gamma H2AX detected in irradiated samples was especially high after irradiation with 12 and 16 Gy. Dose dependent increase of apoptosis was detected 48 hour after irradiation by flow-cytometry, with the maximum value of 20.4% after irradiation with 16 Gy, and the apoptotic index of 9.3. Pro-apoptotic effects of carbon ion beams were confirmed by changes of key molecules of the mitochondrial apoptotic pathway, p53 protein expression, Bax/Bcl-2 ratio and caspase-3 activation.",
journal = "Nuclear technology and radiation protection",
title = "Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells",
volume = "28",
number = "2",
pages = "195-203",
doi = "10.2298/NTRP1302195K"
}
Korićanac, L., Žakula, J., Keta, O. D., Cirrone, G. A. P., Cuttone, G., Ristić-Fira, A.,& Petrović, I. M.. (2013). Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells. in Nuclear technology and radiation protection, 28(2), 195-203.
https://doi.org/10.2298/NTRP1302195K
Korićanac L, Žakula J, Keta OD, Cirrone GAP, Cuttone G, Ristić-Fira A, Petrović IM. Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells. in Nuclear technology and radiation protection. 2013;28(2):195-203.
doi:10.2298/NTRP1302195K .
Korićanac, Lela, Žakula, Jelena, Keta, Otilija D., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells" in Nuclear technology and radiation protection, 28, no. 2 (2013):195-203,
https://doi.org/10.2298/NTRP1302195K . .
2
2
2

Sensitivity of lung carcinoma cells to y-rays and erlotinib

Keta, Otilija D.; Bulat, Tanja M.; Korićanac, Lela; Todorović, Dragana; Privitera, G.; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(Society of Physical Chemists of Serbia, 2012)

TY  - CONF
AU  - Keta, Otilija D.
AU  - Bulat, Tanja M.
AU  - Korićanac, Lela
AU  - Todorović, Dragana
AU  - Privitera, G.
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9300
AB  - In order to increase radio-sensitivity of human lung adenocarcinoma NCI-H1568
cells, targeted therapy drug, erlotinib was used. The impact of radiation and
erlotinib on cell behaviour was analyzed using three biological endpoints.
Irradiations with γ-rays resulted in reduction of cell survival, viability and
proliferation. Erlotinib significantly inhibited cell growth and proliferation
capacity. Combined treatments with radiation and erlotinib showed high level of
reduction of cell viability and proliferation. Preliminary data encourage further
investigations of mechanisms underlying the radiation responses enhanced by
erlotinib.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
T1  - Sensitivity of lung carcinoma cells to y-rays and erlotinib
VL  - 1
SP  - 382
EP  - 384
ER  - 
@conference{
author = "Keta, Otilija D. and Bulat, Tanja M. and Korićanac, Lela and Todorović, Dragana and Privitera, G. and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2012",
abstract = "In order to increase radio-sensitivity of human lung adenocarcinoma NCI-H1568
cells, targeted therapy drug, erlotinib was used. The impact of radiation and
erlotinib on cell behaviour was analyzed using three biological endpoints.
Irradiations with γ-rays resulted in reduction of cell survival, viability and
proliferation. Erlotinib significantly inhibited cell growth and proliferation
capacity. Combined treatments with radiation and erlotinib showed high level of
reduction of cell viability and proliferation. Preliminary data encourage further
investigations of mechanisms underlying the radiation responses enhanced by
erlotinib.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry",
title = "Sensitivity of lung carcinoma cells to y-rays and erlotinib",
volume = "1",
pages = "382-384"
}
Keta, O. D., Bulat, T. M., Korićanac, L., Todorović, D., Privitera, G., Petrović, I. M.,& Ristić-Fira, A.. (2012). Sensitivity of lung carcinoma cells to y-rays and erlotinib. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 382-384.
Keta OD, Bulat TM, Korićanac L, Todorović D, Privitera G, Petrović IM, Ristić-Fira A. Sensitivity of lung carcinoma cells to y-rays and erlotinib. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry. 2012;1:382-384..
Keta, Otilija D., Bulat, Tanja M., Korićanac, Lela, Todorović, Dragana, Privitera, G., Petrović, Ivan M., Ristić-Fira, Aleksandra, "Sensitivity of lung carcinoma cells to y-rays and erlotinib" in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry, 1 (2012):382-384.

Kinetics of dsb induction and changes in cell cycle regulation in melanoma cells after ionizing radiation

Bulat, Tanja M.; Keta, Otilija D.; Korićanac, Lela; Todorović, Dragana; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(Society of Physical Chemists of Serbia, 2012)

TY  - CONF
AU  - Bulat, Tanja M.
AU  - Keta, Otilija D.
AU  - Korićanac, Lela
AU  - Todorović, Dragana
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9299
AB  - The effects of γ-rays on the DNA level, i.e. formation of double-strand breaks and
expression of p21 were studied in vitro on the human HTB 140 melanoma cells.
Cells were exposed to the dose range from 2 to 16 Gy. Effects were analyzed 30
min, 2, 6 and 24 h after irradiation. It has been shown that the level of
phosphorylated histone H2AX (γH2AX) is time- and dose-dependent, as well as
the expression of p21.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
T1  - Kinetics of dsb induction and changes in cell cycle regulation in melanoma cells after ionizing radiation
VL  - 1
SP  - 379
EP  - 381
ER  - 
@conference{
author = "Bulat, Tanja M. and Keta, Otilija D. and Korićanac, Lela and Todorović, Dragana and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2012",
abstract = "The effects of γ-rays on the DNA level, i.e. formation of double-strand breaks and
expression of p21 were studied in vitro on the human HTB 140 melanoma cells.
Cells were exposed to the dose range from 2 to 16 Gy. Effects were analyzed 30
min, 2, 6 and 24 h after irradiation. It has been shown that the level of
phosphorylated histone H2AX (γH2AX) is time- and dose-dependent, as well as
the expression of p21.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry",
title = "Kinetics of dsb induction and changes in cell cycle regulation in melanoma cells after ionizing radiation",
volume = "1",
pages = "379-381"
}
Bulat, T. M., Keta, O. D., Korićanac, L., Todorović, D., Petrović, I. M.,& Ristić-Fira, A.. (2012). Kinetics of dsb induction and changes in cell cycle regulation in melanoma cells after ionizing radiation. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 379-381.
Bulat TM, Keta OD, Korićanac L, Todorović D, Petrović IM, Ristić-Fira A. Kinetics of dsb induction and changes in cell cycle regulation in melanoma cells after ionizing radiation. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry. 2012;1:379-381..
Bulat, Tanja M., Keta, Otilija D., Korićanac, Lela, Todorović, Dragana, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Kinetics of dsb induction and changes in cell cycle regulation in melanoma cells after ionizing radiation" in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry, 1 (2012):379-381.

Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET

Petrović, Ivan M.; Ristić-Fira, Aleksandra; Todorović, Dragana; Korićanac, Lela; Žakula, Jelena; Cirrone, Giuseppe Antonio Pablo; Romano, Francesco; Cuttone, Giacomo

(2012)

TY  - CONF
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Dragana
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8643
C3  - Radiotherapy and Oncology
T1  - Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET
VL  - 102
SP  - S108
EP  - S109
DO  - 10.1016/S0167-8140(12)70185-8
ER  - 
@conference{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Dragana and Korićanac, Lela and Žakula, Jelena and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo",
year = "2012",
journal = "Radiotherapy and Oncology",
title = "Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET",
volume = "102",
pages = "S108-S109",
doi = "10.1016/S0167-8140(12)70185-8"
}
Petrović, I. M., Ristić-Fira, A., Todorović, D., Korićanac, L., Žakula, J., Cirrone, G. A. P., Romano, F.,& Cuttone, G.. (2012). Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET. in Radiotherapy and Oncology, 102, S108-S109.
https://doi.org/10.1016/S0167-8140(12)70185-8
Petrović IM, Ristić-Fira A, Todorović D, Korićanac L, Žakula J, Cirrone GAP, Romano F, Cuttone G. Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET. in Radiotherapy and Oncology. 2012;102:S108-S109.
doi:10.1016/S0167-8140(12)70185-8 .
Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Dragana, Korićanac, Lela, Žakula, Jelena, Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, "Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET" in Radiotherapy and Oncology, 102 (2012):S108-S109,
https://doi.org/10.1016/S0167-8140(12)70185-8 . .
1

Response of human lung adenocarcinoma cells to proton radiation and erlotinib

Ristić-Fira, Aleksandra; Petrović, Ivan M.; Todorović, Dragana; Korićanac, Lela; Keta, Otilija D.; Bulat, Tanja M.; Cirrone, Giuseppe Antonio Pablo; Romano, Francesco; Cuttone, Giacomo

(2012)

TY  - CONF
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
AU  - Todorović, Dragana
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Bulat, Tanja M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8642
C3  - Radiotherapy and Oncology
T1  - Response of human lung adenocarcinoma cells to proton radiation and erlotinib
VL  - 102
SP  - S106
EP  - S107
DO  - 10.1016/S0167-8140(12)70182-2
ER  - 
@conference{
author = "Ristić-Fira, Aleksandra and Petrović, Ivan M. and Todorović, Dragana and Korićanac, Lela and Keta, Otilija D. and Bulat, Tanja M. and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo",
year = "2012",
journal = "Radiotherapy and Oncology",
title = "Response of human lung adenocarcinoma cells to proton radiation and erlotinib",
volume = "102",
pages = "S106-S107",
doi = "10.1016/S0167-8140(12)70182-2"
}
Ristić-Fira, A., Petrović, I. M., Todorović, D., Korićanac, L., Keta, O. D., Bulat, T. M., Cirrone, G. A. P., Romano, F.,& Cuttone, G.. (2012). Response of human lung adenocarcinoma cells to proton radiation and erlotinib. in Radiotherapy and Oncology, 102, S106-S107.
https://doi.org/10.1016/S0167-8140(12)70182-2
Ristić-Fira A, Petrović IM, Todorović D, Korićanac L, Keta OD, Bulat TM, Cirrone GAP, Romano F, Cuttone G. Response of human lung adenocarcinoma cells to proton radiation and erlotinib. in Radiotherapy and Oncology. 2012;102:S106-S107.
doi:10.1016/S0167-8140(12)70182-2 .
Ristić-Fira, Aleksandra, Petrović, Ivan M., Todorović, Dragana, Korićanac, Lela, Keta, Otilija D., Bulat, Tanja M., Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, "Response of human lung adenocarcinoma cells to proton radiation and erlotinib" in Radiotherapy and Oncology, 102 (2012):S106-S107,
https://doi.org/10.1016/S0167-8140(12)70182-2 . .

Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line

Korićanac, Lela; Žakula, Jelena; Cirrone, Giuseppe Antonio Pablo; Privitera, Giuseppe; Cuttone, Giacomo; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(2012)

TY  - JOUR
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Privitera, Giuseppe
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8644
AB  - The effects of combined treatments with fotemustine and proton radiation on cell proliferation and induction of apoptosis have been analyzed in this study. HTB140 human melanoma cells were treated with fotemustine (100, 250 M) 24 h prior to irradiation (12, 16 Gy). The cells were irradiated in the middle of a therapeutic 62 MeV proton spread-out Bragg peak. An efficiency of applied treatments was observed throughout the evaluation of the cell proliferation 7 days after proton irradiation. The combined treatments with fotemustine and protons resulted in a greater antiproliferative response than each treatment alone. The number of apoptotic cells was estimated after 6 or 48 h using flow cytometry. The highest percentage of apoptotic cells was obtained 48 h after treatment with 250 M fotemustine and protons. Western blot analysis showed that induction of apoptosis was associated with p53 and Bax up regulation, and Bcl-2 down regulation. The induction of a caspase-3 activity and cleavage of PARP were clearly observed. These data indicate that a combined application of FM and proton irradiation is more effective in reducing melanoma cell proliferation and the induction of apoptosis, suggesting that FM can increase the radio-sensitivity of HTB140 melanoma cells.
T2  - Advanced Science Letters
T1  - Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line
VL  - 5
IS  - 2
SP  - 552
EP  - 559
DO  - 10.1166/asl.2012.2150
ER  - 
@article{
author = "Korićanac, Lela and Žakula, Jelena and Cirrone, Giuseppe Antonio Pablo and Privitera, Giuseppe and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2012",
abstract = "The effects of combined treatments with fotemustine and proton radiation on cell proliferation and induction of apoptosis have been analyzed in this study. HTB140 human melanoma cells were treated with fotemustine (100, 250 M) 24 h prior to irradiation (12, 16 Gy). The cells were irradiated in the middle of a therapeutic 62 MeV proton spread-out Bragg peak. An efficiency of applied treatments was observed throughout the evaluation of the cell proliferation 7 days after proton irradiation. The combined treatments with fotemustine and protons resulted in a greater antiproliferative response than each treatment alone. The number of apoptotic cells was estimated after 6 or 48 h using flow cytometry. The highest percentage of apoptotic cells was obtained 48 h after treatment with 250 M fotemustine and protons. Western blot analysis showed that induction of apoptosis was associated with p53 and Bax up regulation, and Bcl-2 down regulation. The induction of a caspase-3 activity and cleavage of PARP were clearly observed. These data indicate that a combined application of FM and proton irradiation is more effective in reducing melanoma cell proliferation and the induction of apoptosis, suggesting that FM can increase the radio-sensitivity of HTB140 melanoma cells.",
journal = "Advanced Science Letters",
title = "Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line",
volume = "5",
number = "2",
pages = "552-559",
doi = "10.1166/asl.2012.2150"
}
Korićanac, L., Žakula, J., Cirrone, G. A. P., Privitera, G., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2012). Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line. in Advanced Science Letters, 5(2), 552-559.
https://doi.org/10.1166/asl.2012.2150
Korićanac L, Žakula J, Cirrone GAP, Privitera G, Cuttone G, Petrović IM, Ristić-Fira A. Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line. in Advanced Science Letters. 2012;5(2):552-559.
doi:10.1166/asl.2012.2150 .
Korićanac, Lela, Žakula, Jelena, Cirrone, Giuseppe Antonio Pablo, Privitera, Giuseppe, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line" in Advanced Science Letters, 5, no. 2 (2012):552-559,
https://doi.org/10.1166/asl.2012.2150 . .
1
1

Proton Inactivation of Melanomacells Enhanced By Fotemustine

Ristić-Fira, Aleksandra; Korićanac, Lela; Žakula, Jelena; Keta, Otilija D.; Iannolo, Gioacchin; Cuttone, Giacomo; Petrović, Ivan M.

(2011)

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Keta, Otilija D.
AU  - Iannolo, Gioacchin
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6905
AB  - Response of human HTB140 melanoma cells to proton irradiation in combination with fotemustine (FM) was investigated. Effects of these agents were analysed on cell proliferation and induction of apoptosis. Cells pretreated with 100- or 250-mu M of FM were irradiated in the middle of the therapeutic 62-MeV proton spread-out Bragg peak, with a dose of 16 Gy. All treatments reduced proliferation and survival of melanoma cells. The most pronounced effects of the combined treatment were obtained for cell survivals. The level of apoptosis increased after all applied treatments. Particularly good pro-apoptotic effect was achieved when proton irradiation was combined with 250 mu M of FM. This was followed by the increased expression of p53 gene. The obtained results have shown that combined application of FM and protons significantly reduced growth of this resistant melanoma cell line.
T2  - Radiation Protection Dosimetry
T1  - Proton Inactivation of Melanomacells Enhanced By Fotemustine
VL  - 143
IS  - 2-4
SP  - 503
EP  - 507
DO  - 10.1093/rpd/ncq527
ER  - 
@article{
author = "Ristić-Fira, Aleksandra and Korićanac, Lela and Žakula, Jelena and Keta, Otilija D. and Iannolo, Gioacchin and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2011",
abstract = "Response of human HTB140 melanoma cells to proton irradiation in combination with fotemustine (FM) was investigated. Effects of these agents were analysed on cell proliferation and induction of apoptosis. Cells pretreated with 100- or 250-mu M of FM were irradiated in the middle of the therapeutic 62-MeV proton spread-out Bragg peak, with a dose of 16 Gy. All treatments reduced proliferation and survival of melanoma cells. The most pronounced effects of the combined treatment were obtained for cell survivals. The level of apoptosis increased after all applied treatments. Particularly good pro-apoptotic effect was achieved when proton irradiation was combined with 250 mu M of FM. This was followed by the increased expression of p53 gene. The obtained results have shown that combined application of FM and protons significantly reduced growth of this resistant melanoma cell line.",
journal = "Radiation Protection Dosimetry",
title = "Proton Inactivation of Melanomacells Enhanced By Fotemustine",
volume = "143",
number = "2-4",
pages = "503-507",
doi = "10.1093/rpd/ncq527"
}
Ristić-Fira, A., Korićanac, L., Žakula, J., Keta, O. D., Iannolo, G., Cuttone, G.,& Petrović, I. M.. (2011). Proton Inactivation of Melanomacells Enhanced By Fotemustine. in Radiation Protection Dosimetry, 143(2-4), 503-507.
https://doi.org/10.1093/rpd/ncq527
Ristić-Fira A, Korićanac L, Žakula J, Keta OD, Iannolo G, Cuttone G, Petrović IM. Proton Inactivation of Melanomacells Enhanced By Fotemustine. in Radiation Protection Dosimetry. 2011;143(2-4):503-507.
doi:10.1093/rpd/ncq527 .
Ristić-Fira, Aleksandra, Korićanac, Lela, Žakula, Jelena, Keta, Otilija D., Iannolo, Gioacchin, Cuttone, Giacomo, Petrović, Ivan M., "Proton Inactivation of Melanomacells Enhanced By Fotemustine" in Radiation Protection Dosimetry, 143, no. 2-4 (2011):503-507,
https://doi.org/10.1093/rpd/ncq527 . .
1
2
2

Radio-sensitivity of human melanoma, ovarian and lung carcinoma cells to gamma radiation

Keta, Otilija D.; Korićanac, Lela; Žakula, Jelena; Popović, Nataša; Cuttone, Giacomo; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(Society of Physical Chemists of Serbia, 2010)

TY  - CONF
AU  - Keta, Otilija D.
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Popović, Nataša
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9314
AB  - Radio-sensitivity of human melanoma, ovarian and lung cancer cells after the
exposure to gamma-rays was studied using three different methods. The results
showed that gamma rays reduce the number of viable cells for all analyzed cell
lines. However, these cells display high level of radio-resistance. The highest
radio-sensitivity was attained for the CRL5876 lung cells, while the most sensitive
assay was the clonogenic assay.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Radio-sensitivity of human melanoma, ovarian and lung carcinoma cells to gamma radiation
ER  - 
@conference{
author = "Keta, Otilija D. and Korićanac, Lela and Žakula, Jelena and Popović, Nataša and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2010",
abstract = "Radio-sensitivity of human melanoma, ovarian and lung cancer cells after the
exposure to gamma-rays was studied using three different methods. The results
showed that gamma rays reduce the number of viable cells for all analyzed cell
lines. However, these cells display high level of radio-resistance. The highest
radio-sensitivity was attained for the CRL5876 lung cells, while the most sensitive
assay was the clonogenic assay.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Radio-sensitivity of human melanoma, ovarian and lung carcinoma cells to gamma radiation"
}
Keta, O. D., Korićanac, L., Žakula, J., Popović, N., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2010). Radio-sensitivity of human melanoma, ovarian and lung carcinoma cells to gamma radiation. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
Keta OD, Korićanac L, Žakula J, Popović N, Cuttone G, Petrović IM, Ristić-Fira A. Radio-sensitivity of human melanoma, ovarian and lung carcinoma cells to gamma radiation. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;..
Keta, Otilija D., Korićanac, Lela, Žakula, Jelena, Popović, Nataša, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Radio-sensitivity of human melanoma, ovarian and lung carcinoma cells to gamma radiation" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010).

Carbon ion beam as inducer of melanoma cell apoptosis

Žakula, Jelena; Korićanac, Lela; Keta, Otilija D.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Ristić-Fira, Aleksandra; Petrović, Ivan M.

(Society of Physical Chemists of Serbia, 2010)

TY  - CONF
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9325
AB  - In vitro effect of carbon ions on apoptosis was studied. The human melanoma
HTB140 cells were irradiated with the 62 MeV/u 12C ion beam. Percentage of
apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic
indexes were calculated. The expression of apoptosis-associated proteins, p53, Bax
and Bcl-2 was estimated by Western blot analyses. A dose dependent increase of
apoptosis was revealed, with the maximum value of 17 % after irradiation with 16
Gy, and the apoptotic index of 7.7. Pro-apoptotic effects of carbon ion beams were
confirmed by the detected changes of key regulators of the mitochondrial apoptotic
pathway, the p53 protein expression and the Bax/Bcl-2 ratio.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Carbon ion beam as inducer of melanoma cell apoptosis
ER  - 
@conference{
author = "Žakula, Jelena and Korićanac, Lela and Keta, Otilija D. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra and Petrović, Ivan M.",
year = "2010",
abstract = "In vitro effect of carbon ions on apoptosis was studied. The human melanoma
HTB140 cells were irradiated with the 62 MeV/u 12C ion beam. Percentage of
apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic
indexes were calculated. The expression of apoptosis-associated proteins, p53, Bax
and Bcl-2 was estimated by Western blot analyses. A dose dependent increase of
apoptosis was revealed, with the maximum value of 17 % after irradiation with 16
Gy, and the apoptotic index of 7.7. Pro-apoptotic effects of carbon ion beams were
confirmed by the detected changes of key regulators of the mitochondrial apoptotic
pathway, the p53 protein expression and the Bax/Bcl-2 ratio.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Carbon ion beam as inducer of melanoma cell apoptosis"
}
Žakula, J., Korićanac, L., Keta, O. D., Cirrone, G. A. P., Cuttone, G., Ristić-Fira, A.,& Petrović, I. M.. (2010). Carbon ion beam as inducer of melanoma cell apoptosis. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
Žakula J, Korićanac L, Keta OD, Cirrone GAP, Cuttone G, Ristić-Fira A, Petrović IM. Carbon ion beam as inducer of melanoma cell apoptosis. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;..
Žakula, Jelena, Korićanac, Lela, Keta, Otilija D., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Carbon ion beam as inducer of melanoma cell apoptosis" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010).

Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak

Petrović, Ivan M.; Ristić-Fira, Aleksandra; Todorović, Danijela V.; Korićanac, Lela; Valastro, Lucia; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo

(2010)

TY  - JOUR
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Korićanac, Lela
AU  - Valastro, Lucia
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
PY  - 2010
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4101
AB  - Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was at its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer.
T2  - International Journal of Radiation Biology
T1  - Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak
VL  - 86
IS  - 9
SP  - 742
EP  - 751
DO  - 10.3109/09553002.2010.481322
ER  - 
@article{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V. and Korićanac, Lela and Valastro, Lucia and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo",
year = "2010",
abstract = "Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was at its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer.",
journal = "International Journal of Radiation Biology",
title = "Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak",
volume = "86",
number = "9",
pages = "742-751",
doi = "10.3109/09553002.2010.481322"
}
Petrović, I. M., Ristić-Fira, A., Todorović, D. V., Korićanac, L., Valastro, L., Cirrone, G. A. P.,& Cuttone, G.. (2010). Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak. in International Journal of Radiation Biology, 86(9), 742-751.
https://doi.org/10.3109/09553002.2010.481322
Petrović IM, Ristić-Fira A, Todorović DV, Korićanac L, Valastro L, Cirrone GAP, Cuttone G. Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak. in International Journal of Radiation Biology. 2010;86(9):742-751.
doi:10.3109/09553002.2010.481322 .
Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., Korićanac, Lela, Valastro, Lucia, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak" in International Journal of Radiation Biology, 86, no. 9 (2010):742-751,
https://doi.org/10.3109/09553002.2010.481322 . .
34
35
34

Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab

Korićanac, Lela; Žakula, Jelena; Petrović, Ivan M.; Valastro, Lucia M.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Ristić-Fira, Aleksandra

(2010)

TY  - JOUR
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Petrović, Ivan M.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
PY  - 2010
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4021
AB  - Background: Metastatic melanoma is one of the most aggressive tumours and is also very resistant to current therapeutic approaches. The aim of this investigation was the in vitro study of the anti-proliferative effects of fotemustine (FM; 100 and 250 mu M), bevacizumab (5 mu g/ml) and proton irradiation (12 and 16 Gy) on resistant HTB140 human melanoma cells. Methods: Viability was estimated by sulphorhodamine B assay, while cell proliferation was analyzed by 5-bromo-2-deoxyuridine assay. Cell cycle distribution and apoptosis were examined using flow cytometry. Results: Cell viability and proliferation were reduced after all applied treatments. The level of apoptosis significantly increased after treatment with FM, protons or a combination of all agents, while the apoptotic index ranged from 1.2 to 9.2. Proton irradiation, as well as combined treatment with bevacizumab and protons or 100 mu M FM, bevacizumab and protons, have reduced melanoma cell proliferation through the induction of G1 phase arrest. Single FM (250 mu M) or bevacizumab treatment and their combination, as well as the joint application of these 2 agents with protons, reduced cell proliferation and provoked G2 phase accumulation. Conclusion: The analyzed treatments reduced cell viability and proliferation, triggered G1 or G2 cell cycle phase accumulation and stimulated apoptotic cell death. Copyright (C) 2010 S. Karger AG, Basel
T2  - Chemotherapy
T1  - Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab
VL  - 56
IS  - 3
SP  - 214
EP  - 222
DO  - 10.1159/000316333
ER  - 
@article{
author = "Korićanac, Lela and Žakula, Jelena and Petrović, Ivan M. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra",
year = "2010",
abstract = "Background: Metastatic melanoma is one of the most aggressive tumours and is also very resistant to current therapeutic approaches. The aim of this investigation was the in vitro study of the anti-proliferative effects of fotemustine (FM; 100 and 250 mu M), bevacizumab (5 mu g/ml) and proton irradiation (12 and 16 Gy) on resistant HTB140 human melanoma cells. Methods: Viability was estimated by sulphorhodamine B assay, while cell proliferation was analyzed by 5-bromo-2-deoxyuridine assay. Cell cycle distribution and apoptosis were examined using flow cytometry. Results: Cell viability and proliferation were reduced after all applied treatments. The level of apoptosis significantly increased after treatment with FM, protons or a combination of all agents, while the apoptotic index ranged from 1.2 to 9.2. Proton irradiation, as well as combined treatment with bevacizumab and protons or 100 mu M FM, bevacizumab and protons, have reduced melanoma cell proliferation through the induction of G1 phase arrest. Single FM (250 mu M) or bevacizumab treatment and their combination, as well as the joint application of these 2 agents with protons, reduced cell proliferation and provoked G2 phase accumulation. Conclusion: The analyzed treatments reduced cell viability and proliferation, triggered G1 or G2 cell cycle phase accumulation and stimulated apoptotic cell death. Copyright (C) 2010 S. Karger AG, Basel",
journal = "Chemotherapy",
title = "Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab",
volume = "56",
number = "3",
pages = "214-222",
doi = "10.1159/000316333"
}
Korićanac, L., Žakula, J., Petrović, I. M., Valastro, L. M., Cirrone, G. A. P., Cuttone, G.,& Ristić-Fira, A.. (2010). Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab. in Chemotherapy, 56(3), 214-222.
https://doi.org/10.1159/000316333
Korićanac L, Žakula J, Petrović IM, Valastro LM, Cirrone GAP, Cuttone G, Ristić-Fira A. Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab. in Chemotherapy. 2010;56(3):214-222.
doi:10.1159/000316333 .
Korićanac, Lela, Žakula, Jelena, Petrović, Ivan M., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, "Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab" in Chemotherapy, 56, no. 3 (2010):214-222,
https://doi.org/10.1159/000316333 . .
3
3
3

Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation

Ristić-Fira, Aleksandra; Korićanac, Lela; Žakula, Jelena; Valastro, Lucia M.; Iannolo, Gioacchin; Privitera, Giuseppe; Cuttone, Giacomo; Petrović, Ivan M.

(2009)

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Valastro, Lucia M.
AU  - Iannolo, Gioacchin
AU  - Privitera, Giuseppe
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2009
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3702
AB  - Background: Considering that HTB140 melanoma cells have shown a poor response to either protons or alkylating agents, the effects of a combined use of these agents have been analysed. Methods: Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak (SOBP). Irradiation doses were 12 or 16 Gy and are those frequently used in proton therapy. Four days after irradiation cells were treated with fotemustine (FM) or dacarbazine (DTIC). Drug concentrations were 100 and 250 mu M, values close to those that produce 50% of growth inhibition. Cell viability, proliferation, survival and cell cycle distribution were assessed 7 days after irradiation that corresponds to more than six doubling times of HTB140 cells. In this way incubation periods providing the best single effects of drugs (3 days) and protons (7 days) coincided at the same time. Results: Single proton irradiations have reduced the number of cells to similar to 50%. FM caused stronger cell inactivation due to its high toxicity, while the effectiveness of DTIC, that was important at short term, almost vanished with the incubation of 7 days. Cellular mechanisms triggered by proton irradiation differently influenced the final effects of combined treatments. Combination of protons and FM did not improve cell inactivation level achieved by single treatments. A low efficiency of the single DTIC treatment was overcome when DTIC was introduced following proton irradiation, giving better inhibitory effects with respect to the single treatments. Most of the analysed cells were in G1/S phase, viable, active and able to replicate DNA. Conclusion: The obtained results are the consequence of a high resistance of HTB140 melanoma cells to protons and/or drugs. The inactivation level of the HTB140 human melanoma cells after protons, FM or DTIC treatments was not enhanced by their combined application.
T2  - Journal of Experimental and Clinical Cancer Research
T1  - Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation
VL  - 28
DO  - 10.1186/1756-9966-28-50
ER  - 
@article{
author = "Ristić-Fira, Aleksandra and Korićanac, Lela and Žakula, Jelena and Valastro, Lucia M. and Iannolo, Gioacchin and Privitera, Giuseppe and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2009",
abstract = "Background: Considering that HTB140 melanoma cells have shown a poor response to either protons or alkylating agents, the effects of a combined use of these agents have been analysed. Methods: Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak (SOBP). Irradiation doses were 12 or 16 Gy and are those frequently used in proton therapy. Four days after irradiation cells were treated with fotemustine (FM) or dacarbazine (DTIC). Drug concentrations were 100 and 250 mu M, values close to those that produce 50% of growth inhibition. Cell viability, proliferation, survival and cell cycle distribution were assessed 7 days after irradiation that corresponds to more than six doubling times of HTB140 cells. In this way incubation periods providing the best single effects of drugs (3 days) and protons (7 days) coincided at the same time. Results: Single proton irradiations have reduced the number of cells to similar to 50%. FM caused stronger cell inactivation due to its high toxicity, while the effectiveness of DTIC, that was important at short term, almost vanished with the incubation of 7 days. Cellular mechanisms triggered by proton irradiation differently influenced the final effects of combined treatments. Combination of protons and FM did not improve cell inactivation level achieved by single treatments. A low efficiency of the single DTIC treatment was overcome when DTIC was introduced following proton irradiation, giving better inhibitory effects with respect to the single treatments. Most of the analysed cells were in G1/S phase, viable, active and able to replicate DNA. Conclusion: The obtained results are the consequence of a high resistance of HTB140 melanoma cells to protons and/or drugs. The inactivation level of the HTB140 human melanoma cells after protons, FM or DTIC treatments was not enhanced by their combined application.",
journal = "Journal of Experimental and Clinical Cancer Research",
title = "Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation",
volume = "28",
doi = "10.1186/1756-9966-28-50"
}
Ristić-Fira, A., Korićanac, L., Žakula, J., Valastro, L. M., Iannolo, G., Privitera, G., Cuttone, G.,& Petrović, I. M.. (2009). Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation. in Journal of Experimental and Clinical Cancer Research, 28.
https://doi.org/10.1186/1756-9966-28-50
Ristić-Fira A, Korićanac L, Žakula J, Valastro LM, Iannolo G, Privitera G, Cuttone G, Petrović IM. Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation. in Journal of Experimental and Clinical Cancer Research. 2009;28.
doi:10.1186/1756-9966-28-50 .
Ristić-Fira, Aleksandra, Korićanac, Lela, Žakula, Jelena, Valastro, Lucia M., Iannolo, Gioacchin, Privitera, Giuseppe, Cuttone, Giacomo, Petrović, Ivan M., "Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation" in Journal of Experimental and Clinical Cancer Research, 28 (2009),
https://doi.org/10.1186/1756-9966-28-50 . .
4
6
6

Apoptotic ability of carbon ions on a resistant melanoma cell line

Požega, J. J.; Ristić-Fira, Aleksandra; Korićanac, Lela; Valastro, Lucia M.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Petrović, Ivan M.

(2008)

TY  - CONF
AU  - Požega, J. J.
AU  - Ristić-Fira, Aleksandra
AU  - Korićanac, Lela
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2008
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6761
C3  - FEBS Journal
T1  - Apoptotic ability of carbon ions on a resistant melanoma cell line
VL  - 275
SP  - 425
EP  - 425
ER  - 
@conference{
author = "Požega, J. J. and Ristić-Fira, Aleksandra and Korićanac, Lela and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2008",
journal = "FEBS Journal",
title = "Apoptotic ability of carbon ions on a resistant melanoma cell line",
volume = "275",
pages = "425-425"
}
Požega, J. J., Ristić-Fira, A., Korićanac, L., Valastro, L. M., Cirrone, G. A. P., Cuttone, G.,& Petrović, I. M.. (2008). Apoptotic ability of carbon ions on a resistant melanoma cell line. in FEBS Journal, 275, 425-425.
Požega JJ, Ristić-Fira A, Korićanac L, Valastro LM, Cirrone GAP, Cuttone G, Petrović IM. Apoptotic ability of carbon ions on a resistant melanoma cell line. in FEBS Journal. 2008;275:425-425..
Požega, J. J., Ristić-Fira, Aleksandra, Korićanac, Lela, Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Petrović, Ivan M., "Apoptotic ability of carbon ions on a resistant melanoma cell line" in FEBS Journal, 275 (2008):425-425.

Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons

Korićanac, Lela; Petrović, Ivan M.; Požega, J. J.; Valastro, Lucia M.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Ristić-Fira, Aleksandra

(2008)

TY  - CONF
AU  - Korićanac, Lela
AU  - Petrović, Ivan M.
AU  - Požega, J. J.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
PY  - 2008
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6760
C3  - FEBS Journal
T1  - Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons
VL  - 275
SP  - 409
EP  - 409
ER  - 
@conference{
author = "Korićanac, Lela and Petrović, Ivan M. and Požega, J. J. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra",
year = "2008",
journal = "FEBS Journal",
title = "Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons",
volume = "275",
pages = "409-409"
}
Korićanac, L., Petrović, I. M., Požega, J. J., Valastro, L. M., Cirrone, G. A. P., Cuttone, G.,& Ristić-Fira, A.. (2008). Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons. in FEBS Journal, 275, 409-409.
Korićanac L, Petrović IM, Požega JJ, Valastro LM, Cirrone GAP, Cuttone G, Ristić-Fira A. Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons. in FEBS Journal. 2008;275:409-409..
Korićanac, Lela, Petrović, Ivan M., Požega, J. J., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, "Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons" in FEBS Journal, 275 (2008):409-409.

Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line

Ristić-Fira, Aleksandra; Petrović, Ivan M.; Korićanac, Lela; Valastro, Lucia M.; Privitera, Giuseppe; Cuttone, Giacomo

(2008)

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
AU  - Korićanac, Lela
AU  - Valastro, Lucia M.
AU  - Privitera, Giuseppe
AU  - Cuttone, Giacomo
PY  - 2008
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3613
AB  - The correlation between time dependent viabilities, after applying two radiation qualities and two alkylating agents on HTB140 melanoma cells, has been studied. Irradiations were performed with gamma-rays and 62 MeV protons, close to the Bragg peak maximum, delivering doses of 8-24 Gy. Treatments with fotemustine (FM) and dacarbazine (DTIC) were carried out with concentrations of 0.05-2 mM. High radio-resistance of HTB140 cells revealed by a clonogenic assay was confirmed by microtetrasolium and sulforhodamine B, through the surviving fraction at 2 Gy (SF2), being 0.961-0.956 for gamma-rays and 0.931-0.887 for protons. A better efficiency of protons was illustrated by relative biological effectiveness at 2 Gy (RBE), ranging from 1.69 to 1.89. A kinetic study of concentration dependent cytotoxicity indicated that the best effect of the drugs, estimated as the concentration that produces 50% of growth inhibition (IC(50)), was obtained at 48 h, having values of 76 mu M for DTIC and 145 mu M for FM. The cytostatic ability of the drugs pointed out that the presence of DTIC at 24 h, compared to FM, was insufficient to produce an effect. Protons and FM demonstrated their pro apoptotic capacity. Cross-resistance between treatments applied to the HTB140 cells was observed, protons being the most efficient, while DTIC, FM and gamma-rays demonstrated a lower level of cell inactivation. (C) 2008 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. ALL rights reserved.
T2  - Physica Medica
T1  - Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line
VL  - 24
IS  - 4
SP  - 187
EP  - 195
DO  - 10.1016/j.ejmp.2008.04.002
ER  - 
@article{
author = "Ristić-Fira, Aleksandra and Petrović, Ivan M. and Korićanac, Lela and Valastro, Lucia M. and Privitera, Giuseppe and Cuttone, Giacomo",
year = "2008",
abstract = "The correlation between time dependent viabilities, after applying two radiation qualities and two alkylating agents on HTB140 melanoma cells, has been studied. Irradiations were performed with gamma-rays and 62 MeV protons, close to the Bragg peak maximum, delivering doses of 8-24 Gy. Treatments with fotemustine (FM) and dacarbazine (DTIC) were carried out with concentrations of 0.05-2 mM. High radio-resistance of HTB140 cells revealed by a clonogenic assay was confirmed by microtetrasolium and sulforhodamine B, through the surviving fraction at 2 Gy (SF2), being 0.961-0.956 for gamma-rays and 0.931-0.887 for protons. A better efficiency of protons was illustrated by relative biological effectiveness at 2 Gy (RBE), ranging from 1.69 to 1.89. A kinetic study of concentration dependent cytotoxicity indicated that the best effect of the drugs, estimated as the concentration that produces 50% of growth inhibition (IC(50)), was obtained at 48 h, having values of 76 mu M for DTIC and 145 mu M for FM. The cytostatic ability of the drugs pointed out that the presence of DTIC at 24 h, compared to FM, was insufficient to produce an effect. Protons and FM demonstrated their pro apoptotic capacity. Cross-resistance between treatments applied to the HTB140 cells was observed, protons being the most efficient, while DTIC, FM and gamma-rays demonstrated a lower level of cell inactivation. (C) 2008 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. ALL rights reserved.",
journal = "Physica Medica",
title = "Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line",
volume = "24",
number = "4",
pages = "187-195",
doi = "10.1016/j.ejmp.2008.04.002"
}
Ristić-Fira, A., Petrović, I. M., Korićanac, L., Valastro, L. M., Privitera, G.,& Cuttone, G.. (2008). Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line. in Physica Medica, 24(4), 187-195.
https://doi.org/10.1016/j.ejmp.2008.04.002
Ristić-Fira A, Petrović IM, Korićanac L, Valastro LM, Privitera G, Cuttone G. Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line. in Physica Medica. 2008;24(4):187-195.
doi:10.1016/j.ejmp.2008.04.002 .
Ristić-Fira, Aleksandra, Petrović, Ivan M., Korićanac, Lela, Valastro, Lucia M., Privitera, Giuseppe, Cuttone, Giacomo, "Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line" in Physica Medica, 24, no. 4 (2008):187-195,
https://doi.org/10.1016/j.ejmp.2008.04.002 . .
10
11
12

Response of a human melanoma cell line to low and high ionizing radiation

Ristić-Fira, Aleksandra; Todorović, Danijela V.; Korićanac, Lela; Petrović, Ivan M.; Valastro, Lucia M.; Cirrone, Giuseppe Antonio Pablo; Raffaele, Luigi; Cuttone, Giacomo

(2007)

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Korićanac, Lela
AU  - Petrović, Ivan M.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Raffaele, Luigi
AU  - Cuttone, Giacomo
PY  - 2007
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6648
AB  - Effects of single irradiation with gamma rays and protons on human HTB140 melanoma cell growth were compared. Exponentially growing cells were irradiated close to the Bragg peak maximum of the unmodulated 62 MeV protons, as well as with Co-60 gamma rays. Applied doses ranged from 8 to 24 Gy. Viability of cells and proliferation capacity were assessed 7 days after irradiation. Induction of apoptosis and cell cycle phase redistribution were observed 6 and 48 h after irradiation. Significant inhibitory effects of both irradiation qualities were detected 7 days after irradiation. Important reduction of HTB140 cell viability was observed after irradiation with protons. Almost linear and highly significant (P LT 0.001) decrease of cell proliferation was observed 7 days after irradiation with gamma rays and protons, as compared to nonirradiated controls. Protons induced apoptosis, both 6 and 48 h after irradiation. With the increase of post-irradiation incubation time, number of apoptotic cells decreased. Exposure of HTB140 cells to gamma rays did not provoke apoptotic cell death. Important number of cells in G1-S phase, detected by the cell cycle phase redistribution analyses, suggested high metabolic activity of irradiated melanoma cells within the first 48 h. Both irradiation qualities caused modest G2-M arrest 6 and 48 h after irradiation, thus supporting results that illustrated high radioresistance of HTB140 cells.
T2  - Annals of the New York Academy of Sciences
T1  - Response of a human melanoma cell line to low and high ionizing radiation
VL  - 1095
SP  - 165
EP  - 174
DO  - 10.1196/annals.1397.020
ER  - 
@article{
author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Korićanac, Lela and Petrović, Ivan M. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Raffaele, Luigi and Cuttone, Giacomo",
year = "2007",
abstract = "Effects of single irradiation with gamma rays and protons on human HTB140 melanoma cell growth were compared. Exponentially growing cells were irradiated close to the Bragg peak maximum of the unmodulated 62 MeV protons, as well as with Co-60 gamma rays. Applied doses ranged from 8 to 24 Gy. Viability of cells and proliferation capacity were assessed 7 days after irradiation. Induction of apoptosis and cell cycle phase redistribution were observed 6 and 48 h after irradiation. Significant inhibitory effects of both irradiation qualities were detected 7 days after irradiation. Important reduction of HTB140 cell viability was observed after irradiation with protons. Almost linear and highly significant (P LT 0.001) decrease of cell proliferation was observed 7 days after irradiation with gamma rays and protons, as compared to nonirradiated controls. Protons induced apoptosis, both 6 and 48 h after irradiation. With the increase of post-irradiation incubation time, number of apoptotic cells decreased. Exposure of HTB140 cells to gamma rays did not provoke apoptotic cell death. Important number of cells in G1-S phase, detected by the cell cycle phase redistribution analyses, suggested high metabolic activity of irradiated melanoma cells within the first 48 h. Both irradiation qualities caused modest G2-M arrest 6 and 48 h after irradiation, thus supporting results that illustrated high radioresistance of HTB140 cells.",
journal = "Annals of the New York Academy of Sciences",
title = "Response of a human melanoma cell line to low and high ionizing radiation",
volume = "1095",
pages = "165-174",
doi = "10.1196/annals.1397.020"
}
Ristić-Fira, A., Todorović, D. V., Korićanac, L., Petrović, I. M., Valastro, L. M., Cirrone, G. A. P., Raffaele, L.,& Cuttone, G.. (2007). Response of a human melanoma cell line to low and high ionizing radiation. in Annals of the New York Academy of Sciences, 1095, 165-174.
https://doi.org/10.1196/annals.1397.020
Ristić-Fira A, Todorović DV, Korićanac L, Petrović IM, Valastro LM, Cirrone GAP, Raffaele L, Cuttone G. Response of a human melanoma cell line to low and high ionizing radiation. in Annals of the New York Academy of Sciences. 2007;1095:165-174.
doi:10.1196/annals.1397.020 .
Ristić-Fira, Aleksandra, Todorović, Danijela V., Korićanac, Lela, Petrović, Ivan M., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Raffaele, Luigi, Cuttone, Giacomo, "Response of a human melanoma cell line to low and high ionizing radiation" in Annals of the New York Academy of Sciences, 1095 (2007):165-174,
https://doi.org/10.1196/annals.1397.020 . .
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