TY  - JOUR
AU  - Milosavljević, Milutin M.
AU  - Vukićević Ivan M.
AU  - Drmanic, Sasa Z.
AU  - Nikolic, Jasmina B.
AU  - Marinković, Aleksandar D.
AU  - Krstić, Sanja S.
AU  - Petrović, Slobodan D.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1055
AB  - The present study reports a new facile methodology for the synthesis of symmetrical and asymmetrical thioureas by a one-pot reaction of the required amine, carbon disulfide and an oxidant, i.e., hydrogen peroxide, ethylenediaminetetraacetic acid (EDTA)/sodium percarbonate system or air. The structures of the synthesized compounds were confirmed by IR, H-1- and C-13-NMR and MS methods. A reaction mechanism is proposed based on the isolation of reaction intermediate and the determination of their structure. The synthetic benefits of the presented methods are reflected in the operational simplicity, mild reaction conditions, short reaction times, solvent recycling, high purity and yield of the products, absence of dangerous by-products and technological applicability on an industrial scale. Considering the commercial importance of thioureas, it should be emphasized that implementation of the optimal synthesis of thiourea determined in this study for industrial production could provide a concurrent alternative to the existing technologies in use.
T2  - Journal of the Serbian Chemical Society
T1  - Simple one-pot synthesis of thioureas from amines, carbon disulfide and oxidants in water
VL  - 81
IS  - 3
SP  - 219
EP  - 231
DO  - 10.2298/JSC150831087M
ER  - 

@article{
author = "Milosavljević, Milutin M. and Vukićević Ivan M. and Drmanic, Sasa Z. and Nikolic, Jasmina B. and Marinković, Aleksandar D. and Krstić, Sanja S. and Petrović, Slobodan D.",
year = "2016",
abstract = "The present study reports a new facile methodology for the synthesis of symmetrical and asymmetrical thioureas by a one-pot reaction of the required amine, carbon disulfide and an oxidant, i.e., hydrogen peroxide, ethylenediaminetetraacetic acid (EDTA)/sodium percarbonate system or air. The structures of the synthesized compounds were confirmed by IR, H-1- and C-13-NMR and MS methods. A reaction mechanism is proposed based on the isolation of reaction intermediate and the determination of their structure. The synthetic benefits of the presented methods are reflected in the operational simplicity, mild reaction conditions, short reaction times, solvent recycling, high purity and yield of the products, absence of dangerous by-products and technological applicability on an industrial scale. Considering the commercial importance of thioureas, it should be emphasized that implementation of the optimal synthesis of thiourea determined in this study for industrial production could provide a concurrent alternative to the existing technologies in use.",
journal = "Journal of the Serbian Chemical Society",
title = "Simple one-pot synthesis of thioureas from amines, carbon disulfide and oxidants in water",
volume = "81",
number = "3",
pages = "219-231",
doi = "10.2298/JSC150831087M"
}

Milosavljević, M. M., Vukićević Ivan M., Drmanic, S. Z., Nikolic, J. B., Marinković, A. D., Krstić, S. S.,& Petrović, S. D.. (2016). Simple one-pot synthesis of thioureas from amines, carbon disulfide and oxidants in water. in Journal of the Serbian Chemical Society, 81(3), 219-231.
https://doi.org/10.2298/JSC150831087M

Milosavljević MM, Vukićević Ivan M., Drmanic SZ, Nikolic JB, Marinković AD, Krstić SS, Petrović SD. Simple one-pot synthesis of thioureas from amines, carbon disulfide and oxidants in water. in Journal of the Serbian Chemical Society. 2016;81(3):219-231.
doi:10.2298/JSC150831087M .

Milosavljević, Milutin M., Vukićević Ivan M., Drmanic, Sasa Z., Nikolic, Jasmina B., Marinković, Aleksandar D., Krstić, Sanja S., Petrović, Slobodan D., "Simple one-pot synthesis of thioureas from amines, carbon disulfide and oxidants in water" in Journal of the Serbian Chemical Society, 81, no. 3 (2016):219-231,
https://doi.org/10.2298/JSC150831087M . .

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Vasić, Vesna M.
AU  - Bondžić, Aleksandra M.
AU  - Uscumlic, Gordana S.
AU  - Petrovic, Slobodan D.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5599
AB  - Organophosphorus insecticides have been the most applied group of insectcides for the last two decades. Their main toxic effects are related to irreversible inactivation of acetylcholinesterase (AChE). Actually, they covalently bind to serine OH group in the enzyme active site forming phosphorylated enzyme that cannot hydrolyze acetylcholine. Organophosphorus insecticides in the environment undergo the natural degradation pathway including mainly homogeneous and heterogeneous hydrolysis (especially at high pH) generating non-inhibiting products. Additionally, thio organophosphates are easily oxidized by naturally present oxidants and UV light, forming more toxic and stable oxons. Thus, oxidative degradation procedures, generally referred as advanced oxidation processes (AOP), have been applied for their efficient removal from contaminated waters. The most applied bioassays to monitor the organophosphate toxicity, i.e., the detoxification degree during AOP are Vibrio fischeri and AChE bioassays. Vibrio fischeri toxicity test exploits bioluminescence as the measure of luciferase activity of this marine bacterium, whereas AChE bioassay is based on AChE activity inhibition. Both bioanalytical techniques are rapid (several minutes), simple, sensitive and reproducible. Vibrio fischeri test seems to be a versatile indicator of toxic compounds generated in AOP for organophosphorus insecticides degradation. However, detection of neurotoxic AChE inhibitors, which can be formed in AOP of some organophosphates, requires AChE bioassays. Therefore, AChE toxicity test is more appropriate for monitoring the degradation processes of thio organophosphates, because more toxic oxo organophosphates might be formed and overlooked by Vibrio fischeri bioluminescence inhibition. In addition, during organophosphates removal by AOP, compounds with strong genotoxic potential may be formed, which cannot be detected by standard toxicity tests. For this reason, determination of incidence of micronuclei and cell proliferation index in cultivated human lymphocytes and fibroblasts is suitable for evaluation of organophosphorus insecticides and their break down products inducing cytogenetic damage.
T2  - Hemijska industrija
T1  - Organophosphorus Insecticides: Toxic Effects and Bioanalytical Tests for Evaluating Toxicity During Degradation Processes
VL  - 67
IS  - 2
SP  - 217
EP  - 230
DO  - 10.2298/HEMIND120323060C
ER  - 

@article{
author = "Čolović, Mirjana B. and Krstić, Danijela Z. and Vasić, Vesna M. and Bondžić, Aleksandra M. and Uscumlic, Gordana S. and Petrovic, Slobodan D.",
year = "2013",
abstract = "Organophosphorus insecticides have been the most applied group of insectcides for the last two decades. Their main toxic effects are related to irreversible inactivation of acetylcholinesterase (AChE). Actually, they covalently bind to serine OH group in the enzyme active site forming phosphorylated enzyme that cannot hydrolyze acetylcholine. Organophosphorus insecticides in the environment undergo the natural degradation pathway including mainly homogeneous and heterogeneous hydrolysis (especially at high pH) generating non-inhibiting products. Additionally, thio organophosphates are easily oxidized by naturally present oxidants and UV light, forming more toxic and stable oxons. Thus, oxidative degradation procedures, generally referred as advanced oxidation processes (AOP), have been applied for their efficient removal from contaminated waters. The most applied bioassays to monitor the organophosphate toxicity, i.e., the detoxification degree during AOP are Vibrio fischeri and AChE bioassays. Vibrio fischeri toxicity test exploits bioluminescence as the measure of luciferase activity of this marine bacterium, whereas AChE bioassay is based on AChE activity inhibition. Both bioanalytical techniques are rapid (several minutes), simple, sensitive and reproducible. Vibrio fischeri test seems to be a versatile indicator of toxic compounds generated in AOP for organophosphorus insecticides degradation. However, detection of neurotoxic AChE inhibitors, which can be formed in AOP of some organophosphates, requires AChE bioassays. Therefore, AChE toxicity test is more appropriate for monitoring the degradation processes of thio organophosphates, because more toxic oxo organophosphates might be formed and overlooked by Vibrio fischeri bioluminescence inhibition. In addition, during organophosphates removal by AOP, compounds with strong genotoxic potential may be formed, which cannot be detected by standard toxicity tests. For this reason, determination of incidence of micronuclei and cell proliferation index in cultivated human lymphocytes and fibroblasts is suitable for evaluation of organophosphorus insecticides and their break down products inducing cytogenetic damage.",
journal = "Hemijska industrija",
title = "Organophosphorus Insecticides: Toxic Effects and Bioanalytical Tests for Evaluating Toxicity During Degradation Processes",
volume = "67",
number = "2",
pages = "217-230",
doi = "10.2298/HEMIND120323060C"
}

Čolović, M. B., Krstić, D. Z., Vasić, V. M., Bondžić, A. M., Uscumlic, G. S.,& Petrovic, S. D.. (2013). Organophosphorus Insecticides: Toxic Effects and Bioanalytical Tests for Evaluating Toxicity During Degradation Processes. in Hemijska industrija, 67(2), 217-230.
https://doi.org/10.2298/HEMIND120323060C

Čolović MB, Krstić DZ, Vasić VM, Bondžić AM, Uscumlic GS, Petrovic SD. Organophosphorus Insecticides: Toxic Effects and Bioanalytical Tests for Evaluating Toxicity During Degradation Processes. in Hemijska industrija. 2013;67(2):217-230.
doi:10.2298/HEMIND120323060C .

Čolović, Mirjana B., Krstić, Danijela Z., Vasić, Vesna M., Bondžić, Aleksandra M., Uscumlic, Gordana S., Petrovic, Slobodan D., "Organophosphorus Insecticides: Toxic Effects and Bioanalytical Tests for Evaluating Toxicity During Degradation Processes" in Hemijska industrija, 67, no. 2 (2013):217-230,
https://doi.org/10.2298/HEMIND120323060C . .