TY  - JOUR
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Božanić, Dušan K.
AU  - Vodnik, Vesna
AU  - Dimitrijević-Branković, Suzana I.
AU  - Milosavljević, Aleksandar R.
AU  - Kascakova, S.
AU  - Refregiers, M.
AU  - Đoković, Vladimir
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/971
AB  - The investigation of the interaction of silver nanoparticles and live bacteria cells is of particular importance for understanding and controlling their bactericidal properties. In this study, the process of internalization of silver nanoparticles in Escherichia coli cells was followed by means of synchrotron excitation deep ultraviolet (DUV) fluorescence imaging. Antimicrobial nanostructures that can absorb and emit light in the UV region were prepared by functionalization of silver nanoparticles with tryptophan amino acid and used as environmentally sensitive fluorescent probes. The nanostructures were characterized by morphological (TEM) and spectroscopic methods (UV-vis, FTIR, XPS, and photoluminescence). The TEM images and the analyses of the UV-vis spectra suggested that the addition of tryptophan led to the formation of hybrid nanostructures with pronounced eccentricity and larger sizes with respect to that of the initial silver nanoparticles. The DUV imaging showed that it was possible to distinguish the fluorescent signal pertaining to silver-tryptophan nanostructures from the autofluorescence of the bacteria. The spatial resolution of the fluorescence images was 154 nm which was sufficient to perform analyses of the accumulation of the nanostructures within a single bacterium. The DUV imaging results imply that the tryptophan-functionalized silver nanoparticles interact with cell membranes via insertion of the amino acid into the phospholipid bilayer and enter the cells.
T2  - Analyst
T1  - A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution
VL  - 141
IS  - 6
SP  - 1988
EP  - 1996
DO  - 10.1039/c5an02358k
ER  - 

@article{
author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Vodnik, Vesna and Dimitrijević-Branković, Suzana I. and Milosavljević, Aleksandar R. and Kascakova, S. and Refregiers, M. and Đoković, Vladimir",
year = "2016",
abstract = "The investigation of the interaction of silver nanoparticles and live bacteria cells is of particular importance for understanding and controlling their bactericidal properties. In this study, the process of internalization of silver nanoparticles in Escherichia coli cells was followed by means of synchrotron excitation deep ultraviolet (DUV) fluorescence imaging. Antimicrobial nanostructures that can absorb and emit light in the UV region were prepared by functionalization of silver nanoparticles with tryptophan amino acid and used as environmentally sensitive fluorescent probes. The nanostructures were characterized by morphological (TEM) and spectroscopic methods (UV-vis, FTIR, XPS, and photoluminescence). The TEM images and the analyses of the UV-vis spectra suggested that the addition of tryptophan led to the formation of hybrid nanostructures with pronounced eccentricity and larger sizes with respect to that of the initial silver nanoparticles. The DUV imaging showed that it was possible to distinguish the fluorescent signal pertaining to silver-tryptophan nanostructures from the autofluorescence of the bacteria. The spatial resolution of the fluorescence images was 154 nm which was sufficient to perform analyses of the accumulation of the nanostructures within a single bacterium. The DUV imaging results imply that the tryptophan-functionalized silver nanoparticles interact with cell membranes via insertion of the amino acid into the phospholipid bilayer and enter the cells.",
journal = "Analyst",
title = "A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution",
volume = "141",
number = "6",
pages = "1988-1996",
doi = "10.1039/c5an02358k"
}

Dojčilović, R., Pajović, J. D., Božanić, D. K., Vodnik, V., Dimitrijević-Branković, S. I., Milosavljević, A. R., Kascakova, S., Refregiers, M.,& Đoković, V.. (2016). A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution. in Analyst, 141(6), 1988-1996.
https://doi.org/10.1039/c5an02358k

Dojčilović R, Pajović JD, Božanić DK, Vodnik V, Dimitrijević-Branković SI, Milosavljević AR, Kascakova S, Refregiers M, Đoković V. A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution. in Analyst. 2016;141(6):1988-1996.
doi:10.1039/c5an02358k .

Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Vodnik, Vesna, Dimitrijević-Branković, Suzana I., Milosavljević, Aleksandar R., Kascakova, S., Refregiers, M., Đoković, Vladimir, "A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution" in Analyst, 141, no. 6 (2016):1988-1996,
https://doi.org/10.1039/c5an02358k . .