TY  - JOUR
AU  - Petrović, Stefan
AU  - Arsić, Biljana
AU  - Zlatanović, Ivana
AU  - Milićević, Jelena S.
AU  - Glišić, Sanja
AU  - Mitić, Milan
AU  - Đurović-Pejčev, Rada
AU  - Stojanović, Gordana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11025
AB  - In this study, we considered some pesticides as active substances within formulations for the protection of plant-based food in the Republic of Serbia in silico, because these pesticides have not often been investigated in this way previously, and in an analytical way, because there are not very many available fast, cheap, and easy methods for their determination in real agricultural samples. Seven pesticides were detected in selected agricultural products (tomatoes, cucumbers, peppers, and grapes) using the QuEChERS methodology and HPLC-DAD. Standard curves for the investigated pesticides (chlorantraniliprole, methomyl, metalaxyl, thiacloprid, acetamiprid, emamectin benzoate, and cymoxanil) show good linearity, with R2 values from 0.9785 to 0.9996. The HPLC-DAD method is fast, and these pesticides can be determined in real spiked samples in less than 15 min. We further characterized the pesticides we found in food based on physicochemical properties and molecular descriptors to predict the absorption, distribution, metabolism, elimination, and toxicity (ADMET) of the compounds. We summarized the data supporting their effects on humans using various computational tools to determine their potential adverse effects. The results of our prediction study show that all of the selected pesticides considered in this study have good oral bioavailability, and those with high toxicity, therefore, could be harmful to human health. Chlorantraniliprole was shown in a molecular docking study as a good starting point for a new Alzheimer’s disease drug candidate.
T2  - International Journal of Molecular Sciences
T1  - In Silico Investigation of Selected Pesticides and Their Determination in Agricultural Products Using QuEChERS Methodology and HPLC-DAD
VL  - 24
IS  - 9
SP  - 8003
DO  - 10.3390/ijms24098003
ER  - 

@article{
author = "Petrović, Stefan and Arsić, Biljana and Zlatanović, Ivana and Milićević, Jelena S. and Glišić, Sanja and Mitić, Milan and Đurović-Pejčev, Rada and Stojanović, Gordana",
year = "2023",
abstract = "In this study, we considered some pesticides as active substances within formulations for the protection of plant-based food in the Republic of Serbia in silico, because these pesticides have not often been investigated in this way previously, and in an analytical way, because there are not very many available fast, cheap, and easy methods for their determination in real agricultural samples. Seven pesticides were detected in selected agricultural products (tomatoes, cucumbers, peppers, and grapes) using the QuEChERS methodology and HPLC-DAD. Standard curves for the investigated pesticides (chlorantraniliprole, methomyl, metalaxyl, thiacloprid, acetamiprid, emamectin benzoate, and cymoxanil) show good linearity, with R2 values from 0.9785 to 0.9996. The HPLC-DAD method is fast, and these pesticides can be determined in real spiked samples in less than 15 min. We further characterized the pesticides we found in food based on physicochemical properties and molecular descriptors to predict the absorption, distribution, metabolism, elimination, and toxicity (ADMET) of the compounds. We summarized the data supporting their effects on humans using various computational tools to determine their potential adverse effects. The results of our prediction study show that all of the selected pesticides considered in this study have good oral bioavailability, and those with high toxicity, therefore, could be harmful to human health. Chlorantraniliprole was shown in a molecular docking study as a good starting point for a new Alzheimer’s disease drug candidate.",
journal = "International Journal of Molecular Sciences",
title = "In Silico Investigation of Selected Pesticides and Their Determination in Agricultural Products Using QuEChERS Methodology and HPLC-DAD",
volume = "24",
number = "9",
pages = "8003",
doi = "10.3390/ijms24098003"
}

Petrović, S., Arsić, B., Zlatanović, I., Milićević, J. S., Glišić, S., Mitić, M., Đurović-Pejčev, R.,& Stojanović, G.. (2023). In Silico Investigation of Selected Pesticides and Their Determination in Agricultural Products Using QuEChERS Methodology and HPLC-DAD. in International Journal of Molecular Sciences, 24(9), 8003.
https://doi.org/10.3390/ijms24098003

Petrović S, Arsić B, Zlatanović I, Milićević JS, Glišić S, Mitić M, Đurović-Pejčev R, Stojanović G. In Silico Investigation of Selected Pesticides and Their Determination in Agricultural Products Using QuEChERS Methodology and HPLC-DAD. in International Journal of Molecular Sciences. 2023;24(9):8003.
doi:10.3390/ijms24098003 .

Petrović, Stefan, Arsić, Biljana, Zlatanović, Ivana, Milićević, Jelena S., Glišić, Sanja, Mitić, Milan, Đurović-Pejčev, Rada, Stojanović, Gordana, "In Silico Investigation of Selected Pesticides and Their Determination in Agricultural Products Using QuEChERS Methodology and HPLC-DAD" in International Journal of Molecular Sciences, 24, no. 9 (2023):8003,
https://doi.org/10.3390/ijms24098003 . .

TY  - JOUR
AU  - Zlatanović, Ivana
AU  - Stanković, Miroslava
AU  - Stankov Jovanović, Vesna
AU  - Mitić, Violeta
AU  - Zrnzevic, Ivana
AU  - Đorđević, Aleksandra
AU  - Stojanović, Gordana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1466
AB  - This paper reports for the first time the effect of an acetone extract of Umbilicaria crustulosa on the micronucleus distribution of human lymphocytes, and on the cholinesterase activity and antioxidant activity by the cupric ion reducing antioxidant capacity (CUPRAC) method. Additionally, the total phenolic compounds (TPC) and the antioxidant properties were estimated via DPPH, ABTS and TRP assays. Moreover, the antibacterial activity against two Gram-positive and three Gram-negative bacteria were determined. Acetone extract of U. crustulosa at concentration of 1 and 2 mu g mL(-1) decreased a frequency of micronuclei (MN) by 10.8 and 16.8 %, respectively, acting more or slightly less than the synthetic protector amifostine (AMF, WR-2721, 11.4 %, at concentration of 1 mu g mL(-1)). The tested extract did not inhibit cholinesterase activity nor did it exhibit activity toward the examined bacteria. The extract reduced the concentration of DPPH and ABTS radicals by 88.7 and 96.2 %, respectively. Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.6197+/-0.0166 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 19.7641+/-1.6546 mu g trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 350.4188 +/-14.587 mu g gallic acid equivalents (GAE) per mg of dry extract. The results of the present study showed that U. crustulosa acetone extract is a promising candidate for in vivo experiments considering its antioxidant activity and protective effect on human lymphocytes.
T2  - Journal of the Serbian Chemical Society
T1  - Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract
VL  - 82
IS  - 2
SP  - 141
EP  - 150
DO  - 10.2298/JSC161019014Z
ER  - 

@article{
author = "Zlatanović, Ivana and Stanković, Miroslava and Stankov Jovanović, Vesna and Mitić, Violeta and Zrnzevic, Ivana and Đorđević, Aleksandra and Stojanović, Gordana",
year = "2017",
abstract = "This paper reports for the first time the effect of an acetone extract of Umbilicaria crustulosa on the micronucleus distribution of human lymphocytes, and on the cholinesterase activity and antioxidant activity by the cupric ion reducing antioxidant capacity (CUPRAC) method. Additionally, the total phenolic compounds (TPC) and the antioxidant properties were estimated via DPPH, ABTS and TRP assays. Moreover, the antibacterial activity against two Gram-positive and three Gram-negative bacteria were determined. Acetone extract of U. crustulosa at concentration of 1 and 2 mu g mL(-1) decreased a frequency of micronuclei (MN) by 10.8 and 16.8 %, respectively, acting more or slightly less than the synthetic protector amifostine (AMF, WR-2721, 11.4 %, at concentration of 1 mu g mL(-1)). The tested extract did not inhibit cholinesterase activity nor did it exhibit activity toward the examined bacteria. The extract reduced the concentration of DPPH and ABTS radicals by 88.7 and 96.2 %, respectively. Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.6197+/-0.0166 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 19.7641+/-1.6546 mu g trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 350.4188 +/-14.587 mu g gallic acid equivalents (GAE) per mg of dry extract. The results of the present study showed that U. crustulosa acetone extract is a promising candidate for in vivo experiments considering its antioxidant activity and protective effect on human lymphocytes.",
journal = "Journal of the Serbian Chemical Society",
title = "Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract",
volume = "82",
number = "2",
pages = "141-150",
doi = "10.2298/JSC161019014Z"
}

Zlatanović, I., Stanković, M., Stankov Jovanović, V., Mitić, V., Zrnzevic, I., Đorđević, A.,& Stojanović, G.. (2017). Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract. in Journal of the Serbian Chemical Society, 82(2), 141-150.
https://doi.org/10.2298/JSC161019014Z

Zlatanović I, Stanković M, Stankov Jovanović V, Mitić V, Zrnzevic I, Đorđević A, Stojanović G. Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract. in Journal of the Serbian Chemical Society. 2017;82(2):141-150.
doi:10.2298/JSC161019014Z .

Zlatanović, Ivana, Stanković, Miroslava, Stankov Jovanović, Vesna, Mitić, Violeta, Zrnzevic, Ivana, Đorđević, Aleksandra, Stojanović, Gordana, "Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract" in Journal of the Serbian Chemical Society, 82, no. 2 (2017):141-150,
https://doi.org/10.2298/JSC161019014Z . .

TY  - JOUR
AU  - Zrnzevic, Ivana
AU  - Stanković, Miroslava
AU  - Stankov Jovanović, Vesna
AU  - Mitić, Violeta
AU  - Đorđević, Aleksandra
AU  - Zlatanović, Ivana
AU  - Stojanović, Gordana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1656
AB  - In the present investigation, effects of Ramalina capitata acetone extract on micronucleus distribution on human lymphocytes, on cholinesterase activity and antioxidant activity (by the CUPRAC method) were examined, for the first time as well as its HPLC profile. Additionally, total phenolic compounds (TPC), antioxidant properties (estimated via DPPH, ABTS and TRP assays) and antibacterial activity were determined. The predominant phenolic compounds in this extract were evernic, everninic and obtusatic acids. Acetone extract of R. capitata at concentration of 2 mu g mL(-1) decreased a frequency of micronuclei (MN) for 14.8 %. The extract reduces the concentration of DPPH and ABTS radicals for 21.2 and 36.1 % (respectively). Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.4624 +/- 0.1064 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 6.1176 +/- 0.2964 mu g Trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 670.6376 +/- 66.554 mu g galic acid equivalents (GAE) per mg of dry extract. Tested extract at concentration of 2 mg mL(-1) exhibited inhibition effect (5.2 %) on pooled human serum cholinesterase. The antimicrobial assay showed that acetone extract had inhibition effect towards Gram-positive strains. The results of manifested antioxidant activity, reducing the number of micronuclei in human lymphocytes, and antibacterial activity recommends R. capitata extract for further in vivo studies.
T2  - EXCLI Journal
T1  - Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity
VL  - 16
SP  - 679
EP  - 687
DO  - 10.17179/excli2017-301
ER  - 

@article{
author = "Zrnzevic, Ivana and Stanković, Miroslava and Stankov Jovanović, Vesna and Mitić, Violeta and Đorđević, Aleksandra and Zlatanović, Ivana and Stojanović, Gordana",
year = "2017",
abstract = "In the present investigation, effects of Ramalina capitata acetone extract on micronucleus distribution on human lymphocytes, on cholinesterase activity and antioxidant activity (by the CUPRAC method) were examined, for the first time as well as its HPLC profile. Additionally, total phenolic compounds (TPC), antioxidant properties (estimated via DPPH, ABTS and TRP assays) and antibacterial activity were determined. The predominant phenolic compounds in this extract were evernic, everninic and obtusatic acids. Acetone extract of R. capitata at concentration of 2 mu g mL(-1) decreased a frequency of micronuclei (MN) for 14.8 %. The extract reduces the concentration of DPPH and ABTS radicals for 21.2 and 36.1 % (respectively). Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.4624 +/- 0.1064 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 6.1176 +/- 0.2964 mu g Trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 670.6376 +/- 66.554 mu g galic acid equivalents (GAE) per mg of dry extract. Tested extract at concentration of 2 mg mL(-1) exhibited inhibition effect (5.2 %) on pooled human serum cholinesterase. The antimicrobial assay showed that acetone extract had inhibition effect towards Gram-positive strains. The results of manifested antioxidant activity, reducing the number of micronuclei in human lymphocytes, and antibacterial activity recommends R. capitata extract for further in vivo studies.",
journal = "EXCLI Journal",
title = "Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity",
volume = "16",
pages = "679-687",
doi = "10.17179/excli2017-301"
}

Zrnzevic, I., Stanković, M., Stankov Jovanović, V., Mitić, V., Đorđević, A., Zlatanović, I.,& Stojanović, G.. (2017). Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity. in EXCLI Journal, 16, 679-687.
https://doi.org/10.17179/excli2017-301

Zrnzevic I, Stanković M, Stankov Jovanović V, Mitić V, Đorđević A, Zlatanović I, Stojanović G. Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity. in EXCLI Journal. 2017;16:679-687.
doi:10.17179/excli2017-301 .

Zrnzevic, Ivana, Stanković, Miroslava, Stankov Jovanović, Vesna, Mitić, Violeta, Đorđević, Aleksandra, Zlatanović, Ivana, Stojanović, Gordana, "Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity" in EXCLI Journal, 16 (2017):679-687,
https://doi.org/10.17179/excli2017-301 . .