TY  - JOUR
AU  - Petrović, Sandra
AU  - Vujić, Dragana
AU  - Guć-Šćekić, Marija
AU  - Leskovac, Andreja
AU  - Jevtic, Dragana
AU  - Joksić, Gordana
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3751
AB  - Fanconi anemia (FA) is a genetic disease characterized by progressive pancytopenia and cancer susceptibility. The clinical and cellular phenotypes of Fanconi anemia are associated with a set of redox abnormalities, indicating that FA is an oxidative stress-related disorder. Fanconi anemia cells are highly sensitive to DNA clastogen agents, but their response to ionizing radiation is still unclear. The aim of this study was to evaluate the in vitro radiosensitivity of Fanconi anemia homozygotes and heterozygotes, and to assess the contribution of catalase and superoxide dismutase (SOD) to the overall radiobiological response of the cells. The incidence of radiation-induced lymphocyte micronuclei was used as the indicator of radiation sensitivity in vitro, whereas the activity of antioxidant enzymes was determined in erythrocytes. Patients with FA exhibited a two-fold decrease in catalase activity, accompanied by lowered activity of SOD, and increased incidence of baseline micronuclei. In the entire group of patients (with one exception), a reduced yield of radiation-induced micronuclei in lymphocytes was observed, and this was categorized as a radioresistant response. A mild radioresistant in vitro response was also observed in carrier-mothers, accompanied by reduced activity of catalase. The radiosensitivity of carrier-fathers was normal. The results of this study suggest that reduced activity of catalase is an important contributor to the radiobiological response of cells.
T2  - Archives of Biological Sciences
T1  - Influence of Catalase on the Radiosensitivity of Fanconi Anemia Lymphocytes in Vitro
VL  - 61
IS  - 2
SP  - 195
EP  - 204
DO  - 10.2298/ABS0901195P
ER  - 

@article{
author = "Petrović, Sandra and Vujić, Dragana and Guć-Šćekić, Marija and Leskovac, Andreja and Jevtic, Dragana and Joksić, Gordana",
year = "2009",
abstract = "Fanconi anemia (FA) is a genetic disease characterized by progressive pancytopenia and cancer susceptibility. The clinical and cellular phenotypes of Fanconi anemia are associated with a set of redox abnormalities, indicating that FA is an oxidative stress-related disorder. Fanconi anemia cells are highly sensitive to DNA clastogen agents, but their response to ionizing radiation is still unclear. The aim of this study was to evaluate the in vitro radiosensitivity of Fanconi anemia homozygotes and heterozygotes, and to assess the contribution of catalase and superoxide dismutase (SOD) to the overall radiobiological response of the cells. The incidence of radiation-induced lymphocyte micronuclei was used as the indicator of radiation sensitivity in vitro, whereas the activity of antioxidant enzymes was determined in erythrocytes. Patients with FA exhibited a two-fold decrease in catalase activity, accompanied by lowered activity of SOD, and increased incidence of baseline micronuclei. In the entire group of patients (with one exception), a reduced yield of radiation-induced micronuclei in lymphocytes was observed, and this was categorized as a radioresistant response. A mild radioresistant in vitro response was also observed in carrier-mothers, accompanied by reduced activity of catalase. The radiosensitivity of carrier-fathers was normal. The results of this study suggest that reduced activity of catalase is an important contributor to the radiobiological response of cells.",
journal = "Archives of Biological Sciences",
title = "Influence of Catalase on the Radiosensitivity of Fanconi Anemia Lymphocytes in Vitro",
volume = "61",
number = "2",
pages = "195-204",
doi = "10.2298/ABS0901195P"
}

Petrović, S., Vujić, D., Guć-Šćekić, M., Leskovac, A., Jevtic, D.,& Joksić, G.. (2009). Influence of Catalase on the Radiosensitivity of Fanconi Anemia Lymphocytes in Vitro. in Archives of Biological Sciences, 61(2), 195-204.
https://doi.org/10.2298/ABS0901195P

Petrović S, Vujić D, Guć-Šćekić M, Leskovac A, Jevtic D, Joksić G. Influence of Catalase on the Radiosensitivity of Fanconi Anemia Lymphocytes in Vitro. in Archives of Biological Sciences. 2009;61(2):195-204.
doi:10.2298/ABS0901195P .

Petrović, Sandra, Vujić, Dragana, Guć-Šćekić, Marija, Leskovac, Andreja, Jevtic, Dragana, Joksić, Gordana, "Influence of Catalase on the Radiosensitivity of Fanconi Anemia Lymphocytes in Vitro" in Archives of Biological Sciences, 61, no. 2 (2009):195-204,
https://doi.org/10.2298/ABS0901195P . .