TY  - JOUR
AU  - Tepavčević, Snežana
AU  - Milutinovic, Danijela Vojnovic
AU  - Macut, Djuro
AU  - Stojiljković, Mojca D.
AU  - Nikolić, Marina
AU  - Bozic-Antic, Ivana
AU  - Ćulafić, Tijana
AU  - Bjekic-Macut, Jelica
AU  - Matić, Gordana
AU  - Korićanac, Goran
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/710
AB  - Polycystic ovary syndrome (PCOS) is associated with an altered plasma lipid profile and increased risk for cardiovascular diseases. We hypothesized that molecular mechanisms underlying cardiac pathology in PCOS involve changes in expression and subcellular localization of several key proteins involved in cardiac lipid transport and metabolism, such as fatty acid transporter CD36, lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 (PGC1), and carnitine palmitoyltransferase 1 (CPT1). We used the animal model of PCOS obtained by treating female rats with dihydrotestosterone (DHT). Protein levels of CD36, lipin 1, PPAR alpha, PGC1, and antioxidative enzymes were assessed by Western blot in different cardiac cell compartments. Cardiac triglycerides (TG) and lipid peroxidation were also measured. The content of CD36 was decreased in both the cardiac plasma membranes and intracellular pool. On the other hand, total content of cardiac lipin 1 in DHT-treated rats was elevated, in contrast to decreased microsomal lipin 1 content. An increase in nuclear content of lipin 1 was observed together with elevation of nuclear PPAR alpha and PGC1, and an increase in CPT1 expression. However, lipid peroxidation was reduced in the heart, without alterations in antioxidative enzymes expression and cardiac TG content. The results indicate that treatment of female rats with DHT is accompanied by a decrease of fatty acid uptake and a reduction of lipid peroxidation in the heart. The observed elevation of lipin 1, PPAR alpha, PGC1, and CPT1 expression suggests that cardiac fatty acid metabolism is shifted toward mitochondrial beta oxidation.
T2  - Endocrine
T1  - Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome
VL  - 50
IS  - 1
SP  - 193
EP  - 201
DO  - 10.1007/s12020-015-0558-1
ER  - 

@article{
author = "Tepavčević, Snežana and Milutinovic, Danijela Vojnovic and Macut, Djuro and Stojiljković, Mojca D. and Nikolić, Marina and Bozic-Antic, Ivana and Ćulafić, Tijana and Bjekic-Macut, Jelica and Matić, Gordana and Korićanac, Goran",
year = "2015",
abstract = "Polycystic ovary syndrome (PCOS) is associated with an altered plasma lipid profile and increased risk for cardiovascular diseases. We hypothesized that molecular mechanisms underlying cardiac pathology in PCOS involve changes in expression and subcellular localization of several key proteins involved in cardiac lipid transport and metabolism, such as fatty acid transporter CD36, lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 (PGC1), and carnitine palmitoyltransferase 1 (CPT1). We used the animal model of PCOS obtained by treating female rats with dihydrotestosterone (DHT). Protein levels of CD36, lipin 1, PPAR alpha, PGC1, and antioxidative enzymes were assessed by Western blot in different cardiac cell compartments. Cardiac triglycerides (TG) and lipid peroxidation were also measured. The content of CD36 was decreased in both the cardiac plasma membranes and intracellular pool. On the other hand, total content of cardiac lipin 1 in DHT-treated rats was elevated, in contrast to decreased microsomal lipin 1 content. An increase in nuclear content of lipin 1 was observed together with elevation of nuclear PPAR alpha and PGC1, and an increase in CPT1 expression. However, lipid peroxidation was reduced in the heart, without alterations in antioxidative enzymes expression and cardiac TG content. The results indicate that treatment of female rats with DHT is accompanied by a decrease of fatty acid uptake and a reduction of lipid peroxidation in the heart. The observed elevation of lipin 1, PPAR alpha, PGC1, and CPT1 expression suggests that cardiac fatty acid metabolism is shifted toward mitochondrial beta oxidation.",
journal = "Endocrine",
title = "Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome",
volume = "50",
number = "1",
pages = "193-201",
doi = "10.1007/s12020-015-0558-1"
}

Tepavčević, S., Milutinovic, D. V., Macut, D., Stojiljković, M. D., Nikolić, M., Bozic-Antic, I., Ćulafić, T., Bjekic-Macut, J., Matić, G.,& Korićanac, G.. (2015). Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome. in Endocrine, 50(1), 193-201.
https://doi.org/10.1007/s12020-015-0558-1

Tepavčević S, Milutinovic DV, Macut D, Stojiljković MD, Nikolić M, Bozic-Antic I, Ćulafić T, Bjekic-Macut J, Matić G, Korićanac G. Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome. in Endocrine. 2015;50(1):193-201.
doi:10.1007/s12020-015-0558-1 .

Tepavčević, Snežana, Milutinovic, Danijela Vojnovic, Macut, Djuro, Stojiljković, Mojca D., Nikolić, Marina, Bozic-Antic, Ivana, Ćulafić, Tijana, Bjekic-Macut, Jelica, Matić, Gordana, Korićanac, Goran, "Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome" in Endocrine, 50, no. 1 (2015):193-201,
https://doi.org/10.1007/s12020-015-0558-1 . .

TY  - JOUR
AU  - Hrnčić, Dragan
AU  - Rašić-Marković, Aleksandra
AU  - Lekovic, J.
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Macut, D.
AU  - Šušić, Veselinka
AU  - Đurić, Dragan M.
AU  - Stanojlović, Olivera
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6049
AB  - The aim of the study was to examine the effects of chronic exercise training on seizures induced by homocysteine thiolactone (HCT) in adult rats. Rats were assigned to: sedentary control; exercise control; sedentary +HCT; exercise + HCT group. Animals in the exercise groups ran 30 min daily on a treadmill for 30 consecutive days (belt speed 20 m/min), while sedentary rats spent the same time on the treadmill (speed 0 m/min). On the 31st day, the HCT groups received HCT (8.0 mmol/kg), while the control groups received vehicle. Afterwards, convulsive behavior and EEG activity were registered. Lipid peroxidation, superoxide dismutase (SOD) and catalase (CAT) activity were ascertained in the rat hippocampus. No signs of seizures were registered in sedentary and exercise control rats. Seizure latency was increased, while number of seizure episodes and spike-and-wave discharges (SWD) in EEG were decreased in the exercise + HCT compared to the sedentary +HCT group. Seizure incidence, the severity thereof and duration of SWDs were not significantly different between these groups. Exercise partly prevented increase of lipid peroxidation and decrease of the SOD and CAT activity after HCT administration. These results indicate beneficial effects of exercise in model of HCT induced seizures in rats, what could be, at least in part, a consequence of improved antioxidant enzymes activity.
T2  - International Journal of Sports Medicine
T1  - Exercise Decreases Susceptibility to Homocysteine Seizures: the Role of Oxidative Stress
VL  - 35
IS  - 7
SP  - 544
EP  - 550
DO  - 10.1055/s-0033-1357162
ER  - 

@article{
author = "Hrnčić, Dragan and Rašić-Marković, Aleksandra and Lekovic, J. and Krstić, Danijela Z. and Čolović, Mirjana B. and Macut, D. and Šušić, Veselinka and Đurić, Dragan M. and Stanojlović, Olivera",
year = "2014",
abstract = "The aim of the study was to examine the effects of chronic exercise training on seizures induced by homocysteine thiolactone (HCT) in adult rats. Rats were assigned to: sedentary control; exercise control; sedentary +HCT; exercise + HCT group. Animals in the exercise groups ran 30 min daily on a treadmill for 30 consecutive days (belt speed 20 m/min), while sedentary rats spent the same time on the treadmill (speed 0 m/min). On the 31st day, the HCT groups received HCT (8.0 mmol/kg), while the control groups received vehicle. Afterwards, convulsive behavior and EEG activity were registered. Lipid peroxidation, superoxide dismutase (SOD) and catalase (CAT) activity were ascertained in the rat hippocampus. No signs of seizures were registered in sedentary and exercise control rats. Seizure latency was increased, while number of seizure episodes and spike-and-wave discharges (SWD) in EEG were decreased in the exercise + HCT compared to the sedentary +HCT group. Seizure incidence, the severity thereof and duration of SWDs were not significantly different between these groups. Exercise partly prevented increase of lipid peroxidation and decrease of the SOD and CAT activity after HCT administration. These results indicate beneficial effects of exercise in model of HCT induced seizures in rats, what could be, at least in part, a consequence of improved antioxidant enzymes activity.",
journal = "International Journal of Sports Medicine",
title = "Exercise Decreases Susceptibility to Homocysteine Seizures: the Role of Oxidative Stress",
volume = "35",
number = "7",
pages = "544-550",
doi = "10.1055/s-0033-1357162"
}

Hrnčić, D., Rašić-Marković, A., Lekovic, J., Krstić, D. Z., Čolović, M. B., Macut, D., Šušić, V., Đurić, D. M.,& Stanojlović, O.. (2014). Exercise Decreases Susceptibility to Homocysteine Seizures: the Role of Oxidative Stress. in International Journal of Sports Medicine, 35(7), 544-550.
https://doi.org/10.1055/s-0033-1357162

Hrnčić D, Rašić-Marković A, Lekovic J, Krstić DZ, Čolović MB, Macut D, Šušić V, Đurić DM, Stanojlović O. Exercise Decreases Susceptibility to Homocysteine Seizures: the Role of Oxidative Stress. in International Journal of Sports Medicine. 2014;35(7):544-550.
doi:10.1055/s-0033-1357162 .

Hrnčić, Dragan, Rašić-Marković, Aleksandra, Lekovic, J., Krstić, Danijela Z., Čolović, Mirjana B., Macut, D., Šušić, Veselinka, Đurić, Dragan M., Stanojlović, Olivera, "Exercise Decreases Susceptibility to Homocysteine Seizures: the Role of Oxidative Stress" in International Journal of Sports Medicine, 35, no. 7 (2014):544-550,
https://doi.org/10.1055/s-0033-1357162 . .

TY  - JOUR
AU  - Tepavčević, Snežana
AU  - Milutinovic, Danijela Vojnovic
AU  - Macut, Djuro
AU  - Žakula, Zorica
AU  - Nikolić, Marina
AU  - Bozic-Antic, Ivana
AU  - Romić, Snježana Đ.
AU  - Bjekic-Macut, Jelica
AU  - Matić, Gordana
AU  - Korićanac, Goran
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5970
AB  - It is supposed that women with polycystic ovary syndrome (PCOS) are prone to develop cardiovascular disease as a consequence of multiple risk factors that are mostly related to the state of insulin resistance and consequent hyperinsulinemia. In the present study, we evaluated insulin signaling and glucose transporters (GLUT) in cardiac cells of dihydrotestosterone (DHT) treated female rats as an animal model of PCOS. Expression of proteins involved in cardiac insulin signaling pathways and glucose transporters, as well as their phosphorylation or intracellular localization were studied by Western blot analysis in DHT-treated and control rats. Treatment with DHT resulted in increased body mass, absolute mass of the heart, elevated plasma insulin concentration, dyslipidemia and insulin resistance. At the molecular level, DHT treatment did not change protein expression of cardiac insulin receptor and insulin receptor substrate 1, while phosphorylation of the substrate at serine 307 was increased. Unexpectedly, although expression of downstream Akt kinase and its phosphorylation at threonine 308 were not altered, phosphoiylation of Akt at serine 473 was increased in the heart of DHT-treated rats. In contrast, expression and phosphorylation of extracellular signal regulated kinases 1/2 were decreased. Plasma membrane contents of GLUT1 and GLUT4 were decreased, as well as the expression of GLUT4 in cardiac cells at the end of androgen treatment. The obtained results provide evidence for alterations in expression and especially in functional characteristics of insulin signaling molecules and glucose transporters in the heart of DHT-treated rats with PCOS, indicating impaired cardiac insulin action. (c) 2014 Elsevier Ltd. All rights reserved.
T2  - Journal of Steroid Biochemistry and Molecular Biology
T1  - Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome
VL  - 141
SP  - 71
EP  - 76
DO  - 10.1016/j.jsbmb.2014.01.006
ER  - 

@article{
author = "Tepavčević, Snežana and Milutinovic, Danijela Vojnovic and Macut, Djuro and Žakula, Zorica and Nikolić, Marina and Bozic-Antic, Ivana and Romić, Snježana Đ. and Bjekic-Macut, Jelica and Matić, Gordana and Korićanac, Goran",
year = "2014",
abstract = "It is supposed that women with polycystic ovary syndrome (PCOS) are prone to develop cardiovascular disease as a consequence of multiple risk factors that are mostly related to the state of insulin resistance and consequent hyperinsulinemia. In the present study, we evaluated insulin signaling and glucose transporters (GLUT) in cardiac cells of dihydrotestosterone (DHT) treated female rats as an animal model of PCOS. Expression of proteins involved in cardiac insulin signaling pathways and glucose transporters, as well as their phosphorylation or intracellular localization were studied by Western blot analysis in DHT-treated and control rats. Treatment with DHT resulted in increased body mass, absolute mass of the heart, elevated plasma insulin concentration, dyslipidemia and insulin resistance. At the molecular level, DHT treatment did not change protein expression of cardiac insulin receptor and insulin receptor substrate 1, while phosphorylation of the substrate at serine 307 was increased. Unexpectedly, although expression of downstream Akt kinase and its phosphorylation at threonine 308 were not altered, phosphoiylation of Akt at serine 473 was increased in the heart of DHT-treated rats. In contrast, expression and phosphorylation of extracellular signal regulated kinases 1/2 were decreased. Plasma membrane contents of GLUT1 and GLUT4 were decreased, as well as the expression of GLUT4 in cardiac cells at the end of androgen treatment. The obtained results provide evidence for alterations in expression and especially in functional characteristics of insulin signaling molecules and glucose transporters in the heart of DHT-treated rats with PCOS, indicating impaired cardiac insulin action. (c) 2014 Elsevier Ltd. All rights reserved.",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
title = "Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome",
volume = "141",
pages = "71-76",
doi = "10.1016/j.jsbmb.2014.01.006"
}

Tepavčević, S., Milutinovic, D. V., Macut, D., Žakula, Z., Nikolić, M., Bozic-Antic, I., Romić, S. Đ., Bjekic-Macut, J., Matić, G.,& Korićanac, G.. (2014). Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome. in Journal of Steroid Biochemistry and Molecular Biology, 141, 71-76.
https://doi.org/10.1016/j.jsbmb.2014.01.006

Tepavčević S, Milutinovic DV, Macut D, Žakula Z, Nikolić M, Bozic-Antic I, Romić SĐ, Bjekic-Macut J, Matić G, Korićanac G. Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome. in Journal of Steroid Biochemistry and Molecular Biology. 2014;141:71-76.
doi:10.1016/j.jsbmb.2014.01.006 .

Tepavčević, Snežana, Milutinovic, Danijela Vojnovic, Macut, Djuro, Žakula, Zorica, Nikolić, Marina, Bozic-Antic, Ivana, Romić, Snježana Đ., Bjekic-Macut, Jelica, Matić, Gordana, Korićanac, Goran, "Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome" in Journal of Steroid Biochemistry and Molecular Biology, 141 (2014):71-76,
https://doi.org/10.1016/j.jsbmb.2014.01.006 . .

TY  - JOUR
AU  - Mladenovic, Dusan
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Radosavljevic, Tatjana
AU  - Rašić-Marković, Aleksandra
AU  - Hrnčić, Dragan
AU  - Macut, Djuro
AU  - Stanojlović, Olivera
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4696
AB  - Thioacetamide (TAA) exerts hepatotoxic, neurotoxic and carcinogenic effects. The aim of our study was to investigate the effects of TAA on lipid peroxidation and catalase activity in various rat brain regions. Male Wistar rats were divided into following groups: 1. control, saline-treated; 2. thioacetamide-treated groups, TAA(300) (300 mg/kg), TAA(600) (600 mg/kg) and TAA(900) (900 mg/kg). Daily dose of TAA (300 mg/kg) was administered intraperitoneally once (TAA(300)), twice (TAA(600)) and three times (TAA(900)) in consecutive days. Brain samples were collected 24 h after the last dose of TAA and malondialdehyde (MDA) level and catalase activity were determined in cortex, brainstem and hippocampus. MDA level was significantly increased while catalase activity was significantly lower in all brain regions in TAA(900) group in comparison with control group. In TAA(600) MDA level was increased in the brainstem and cortex when compared to control (p LT 0.01). The same dose of TAA(600) mg/kg induced a significant decline in catalase activity in the brainstem and cortex and an increase in its activity in the hippocampus when compared to control (p LT 0.01). In TAA(300) an increase in MDA level was evident only in the brainstem. Catalase activity was significantly higher in the cortex and hippocampus in TAA(300) group in comparison with control (p LT 0.01). Based on these results, it may be concluded that various rat brain regions have different sensitivity to TAA-induced lipid peroxidation with hippocampus being less sensitive than cerebral cortex and brainstem.
T2  - Medicinal Chemistry
T1  - Different Sensitivity of Various Brain Structures to Thioacetamide-Induced Lipid Peroxidation
VL  - 8
IS  - 1
SP  - 52
EP  - 58
DO  - 10.2174/157340612799278603
ER  - 

@article{
author = "Mladenovic, Dusan and Krstić, Danijela Z. and Čolović, Mirjana B. and Radosavljevic, Tatjana and Rašić-Marković, Aleksandra and Hrnčić, Dragan and Macut, Djuro and Stanojlović, Olivera",
year = "2012",
abstract = "Thioacetamide (TAA) exerts hepatotoxic, neurotoxic and carcinogenic effects. The aim of our study was to investigate the effects of TAA on lipid peroxidation and catalase activity in various rat brain regions. Male Wistar rats were divided into following groups: 1. control, saline-treated; 2. thioacetamide-treated groups, TAA(300) (300 mg/kg), TAA(600) (600 mg/kg) and TAA(900) (900 mg/kg). Daily dose of TAA (300 mg/kg) was administered intraperitoneally once (TAA(300)), twice (TAA(600)) and three times (TAA(900)) in consecutive days. Brain samples were collected 24 h after the last dose of TAA and malondialdehyde (MDA) level and catalase activity were determined in cortex, brainstem and hippocampus. MDA level was significantly increased while catalase activity was significantly lower in all brain regions in TAA(900) group in comparison with control group. In TAA(600) MDA level was increased in the brainstem and cortex when compared to control (p LT 0.01). The same dose of TAA(600) mg/kg induced a significant decline in catalase activity in the brainstem and cortex and an increase in its activity in the hippocampus when compared to control (p LT 0.01). In TAA(300) an increase in MDA level was evident only in the brainstem. Catalase activity was significantly higher in the cortex and hippocampus in TAA(300) group in comparison with control (p LT 0.01). Based on these results, it may be concluded that various rat brain regions have different sensitivity to TAA-induced lipid peroxidation with hippocampus being less sensitive than cerebral cortex and brainstem.",
journal = "Medicinal Chemistry",
title = "Different Sensitivity of Various Brain Structures to Thioacetamide-Induced Lipid Peroxidation",
volume = "8",
number = "1",
pages = "52-58",
doi = "10.2174/157340612799278603"
}

Mladenovic, D., Krstić, D. Z., Čolović, M. B., Radosavljevic, T., Rašić-Marković, A., Hrnčić, D., Macut, D.,& Stanojlović, O.. (2012). Different Sensitivity of Various Brain Structures to Thioacetamide-Induced Lipid Peroxidation. in Medicinal Chemistry, 8(1), 52-58.
https://doi.org/10.2174/157340612799278603

Mladenovic D, Krstić DZ, Čolović MB, Radosavljevic T, Rašić-Marković A, Hrnčić D, Macut D, Stanojlović O. Different Sensitivity of Various Brain Structures to Thioacetamide-Induced Lipid Peroxidation. in Medicinal Chemistry. 2012;8(1):52-58.
doi:10.2174/157340612799278603 .

Mladenovic, Dusan, Krstić, Danijela Z., Čolović, Mirjana B., Radosavljevic, Tatjana, Rašić-Marković, Aleksandra, Hrnčić, Dragan, Macut, Djuro, Stanojlović, Olivera, "Different Sensitivity of Various Brain Structures to Thioacetamide-Induced Lipid Peroxidation" in Medicinal Chemistry, 8, no. 1 (2012):52-58,
https://doi.org/10.2174/157340612799278603 . .