TY  - JOUR
AU  - Cabrera-Barjas, Gustavo
AU  - Gallardo, Francisco
AU  - Nešić, Aleksandra
AU  - Taboada, Edelio
AU  - Marican, Adolfo
AU  - Mirabal-Gallardo, Yaneris
AU  - Avila-Salas, Fabian
AU  - Delgado, Nacarid
AU  - de Armas-Ricard, Merly
AU  - Valdes, Oscar
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10701
AB  - In this work, Aspergillus niger and Fusarium culmorum cell wall by-products were chosen as microbial sources for chitin and chitosan production. Both polysaccharides were characterized by FTIR and 13C-CPMAS NMR, but GPC analysis was only performed for chitosan. SEM-EDX analysis was performed to fungal chitosan loaded with metal ions. Chitosan extracted from both fungus had low to medium molecular weight (Mw) and degree of deacetylations (DD) ranging from 65.7-83.3%. Fungal chitosan samples were intended to be used for bioremediation applications. For this purpose, two independent absorption experiments regarding pesticide Dimethoate (DM) and heavy metal ions (Al(III), As(III), Cd(II), Cu(II), Mg(II), Mn(II), Pb(II), Zn(II), Fe(II)) in a complex mixture were carried out. An experimental design considering the solution pH, contact time and chitosan physicochemical properties (DD) were performed. The highest percentage of dimethoate pesticide and selected metal ions absorption was obtained with highest DD chitosan, the contact time of 24 h, pH 6 for metals and pH 4 for pesticide, repectively. Molecular dynamics simulation studies allowed to analyze at the molecular level the chitosan-DM interaction. A higher number of h-bonds were identified as the main interactions that stabilize the affinity of the chitosan-DM complexes. Based on our results, we suggest the use of a multipurpose fungal chitosan system for water bioremediation.
T2  - Journal of Environmental Chemical Engineering
T1  - Utilization of industrial by-product fungal biomass from Aspergillus niger and Fusarium culmorum to obtain biosorbents for removal of pesticide and metal ions from aqueous solutions
VL  - 8
IS  - 5
SP  - 104355
DO  - 10.1016/j.jece.2020.104355
ER  - 

@article{
author = "Cabrera-Barjas, Gustavo and Gallardo, Francisco and Nešić, Aleksandra and Taboada, Edelio and Marican, Adolfo and Mirabal-Gallardo, Yaneris and Avila-Salas, Fabian and Delgado, Nacarid and de Armas-Ricard, Merly and Valdes, Oscar",
year = "2020",
abstract = "In this work, Aspergillus niger and Fusarium culmorum cell wall by-products were chosen as microbial sources for chitin and chitosan production. Both polysaccharides were characterized by FTIR and 13C-CPMAS NMR, but GPC analysis was only performed for chitosan. SEM-EDX analysis was performed to fungal chitosan loaded with metal ions. Chitosan extracted from both fungus had low to medium molecular weight (Mw) and degree of deacetylations (DD) ranging from 65.7-83.3%. Fungal chitosan samples were intended to be used for bioremediation applications. For this purpose, two independent absorption experiments regarding pesticide Dimethoate (DM) and heavy metal ions (Al(III), As(III), Cd(II), Cu(II), Mg(II), Mn(II), Pb(II), Zn(II), Fe(II)) in a complex mixture were carried out. An experimental design considering the solution pH, contact time and chitosan physicochemical properties (DD) were performed. The highest percentage of dimethoate pesticide and selected metal ions absorption was obtained with highest DD chitosan, the contact time of 24 h, pH 6 for metals and pH 4 for pesticide, repectively. Molecular dynamics simulation studies allowed to analyze at the molecular level the chitosan-DM interaction. A higher number of h-bonds were identified as the main interactions that stabilize the affinity of the chitosan-DM complexes. Based on our results, we suggest the use of a multipurpose fungal chitosan system for water bioremediation.",
journal = "Journal of Environmental Chemical Engineering",
title = "Utilization of industrial by-product fungal biomass from Aspergillus niger and Fusarium culmorum to obtain biosorbents for removal of pesticide and metal ions from aqueous solutions",
volume = "8",
number = "5",
pages = "104355",
doi = "10.1016/j.jece.2020.104355"
}

Cabrera-Barjas, G., Gallardo, F., Nešić, A., Taboada, E., Marican, A., Mirabal-Gallardo, Y., Avila-Salas, F., Delgado, N., de Armas-Ricard, M.,& Valdes, O.. (2020). Utilization of industrial by-product fungal biomass from Aspergillus niger and Fusarium culmorum to obtain biosorbents for removal of pesticide and metal ions from aqueous solutions. in Journal of Environmental Chemical Engineering, 8(5), 104355.
https://doi.org/10.1016/j.jece.2020.104355

Cabrera-Barjas G, Gallardo F, Nešić A, Taboada E, Marican A, Mirabal-Gallardo Y, Avila-Salas F, Delgado N, de Armas-Ricard M, Valdes O. Utilization of industrial by-product fungal biomass from Aspergillus niger and Fusarium culmorum to obtain biosorbents for removal of pesticide and metal ions from aqueous solutions. in Journal of Environmental Chemical Engineering. 2020;8(5):104355.
doi:10.1016/j.jece.2020.104355 .

Cabrera-Barjas, Gustavo, Gallardo, Francisco, Nešić, Aleksandra, Taboada, Edelio, Marican, Adolfo, Mirabal-Gallardo, Yaneris, Avila-Salas, Fabian, Delgado, Nacarid, de Armas-Ricard, Merly, Valdes, Oscar, "Utilization of industrial by-product fungal biomass from Aspergillus niger and Fusarium culmorum to obtain biosorbents for removal of pesticide and metal ions from aqueous solutions" in Journal of Environmental Chemical Engineering, 8, no. 5 (2020):104355,
https://doi.org/10.1016/j.jece.2020.104355 . .

TY  - JOUR
AU  - Bernabé, Patricia
AU  - Becherán, Liliam
AU  - Cabrera-Barjas, Gustavo
AU  - Nešić, Aleksandra
AU  - Alburquenque, Claudio
AU  - Tapia, Cecilia V.
AU  - Taboada, Edelio
AU  - Alderete, Joel
AU  - De Los Ríos, Patricio
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10702
AB  - In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time. α-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0–10.4% (w/w). Moreover, chitosan was extracted by alkaline treatment at different temperature and time regimes. Aegla cholchol exoskeleton, chitin and chitosans were characterized by FTIR, TGA, and SEM. Chemical structure of chitin and chitosan was confirmed by NMR spectroscopy. Chitosan molecular weight determinations were carried out by GPC. The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65–201 kDa. The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast. Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis. Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.
T2  - International Journal of Biological Macromolecules
T1  - Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp
VL  - 149
SP  - 962
EP  - 975
DO  - 10.1016/j.ijbiomac.2020.01.126
ER  - 

@article{
author = "Bernabé, Patricia and Becherán, Liliam and Cabrera-Barjas, Gustavo and Nešić, Aleksandra and Alburquenque, Claudio and Tapia, Cecilia V. and Taboada, Edelio and Alderete, Joel and De Los Ríos, Patricio",
year = "2020",
abstract = "In the present work, the chemical composition of the Chilean freshwater crab Aegla cholchol exoskeleton was studied for the first time. α-Chitin was isolated from three main body parts (pincers, legs, carapace), and its content ranged from 9.0–10.4% (w/w). Moreover, chitosan was extracted by alkaline treatment at different temperature and time regimes. Aegla cholchol exoskeleton, chitin and chitosans were characterized by FTIR, TGA, and SEM. Chemical structure of chitin and chitosan was confirmed by NMR spectroscopy. Chitosan molecular weight determinations were carried out by GPC. The obtained chitosan samples had a degree of N-acetylation (DA) between 4 and 15% and molecular weight (Mw) in the range of 65–201 kDa. The antifungal activity of the chitosan samples and the chitooligomer were tested toward twenty isolated clinical strains of Candida yeast. Chitosan with lower DA (4%) and higher molecular weight showed the lowest minimum inhibitory concentration (MIC) values, which was evidenced by the highest antifungal activity toward Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis. Results suggest that Aegla cholchol is an excellent natural source for production of bioactive materials with potential applications in the health system, to prevent infections associated with Candida strains.",
journal = "International Journal of Biological Macromolecules",
title = "Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp",
volume = "149",
pages = "962-975",
doi = "10.1016/j.ijbiomac.2020.01.126"
}

Bernabé, P., Becherán, L., Cabrera-Barjas, G., Nešić, A., Alburquenque, C., Tapia, C. V., Taboada, E., Alderete, J.,& De Los Ríos, P.. (2020). Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp. in International Journal of Biological Macromolecules, 149, 962-975.
https://doi.org/10.1016/j.ijbiomac.2020.01.126

Bernabé P, Becherán L, Cabrera-Barjas G, Nešić A, Alburquenque C, Tapia CV, Taboada E, Alderete J, De Los Ríos P. Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp. in International Journal of Biological Macromolecules. 2020;149:962-975.
doi:10.1016/j.ijbiomac.2020.01.126 .

Bernabé, Patricia, Becherán, Liliam, Cabrera-Barjas, Gustavo, Nešić, Aleksandra, Alburquenque, Claudio, Tapia, Cecilia V., Taboada, Edelio, Alderete, Joel, De Los Ríos, Patricio, "Chilean crab (Aegla cholchol) as a new source of chitin and chitosan with antifungal properties against Candida spp" in International Journal of Biological Macromolecules, 149 (2020):962-975,
https://doi.org/10.1016/j.ijbiomac.2020.01.126 . .