TY  - JOUR
AU  - Ognjanović, Miloš
AU  - Jaćimović, Željko
AU  - Kosović-Perutović, Milica
AU  - Besu Žižak, Irina
AU  - Stanojković, Tatjana
AU  - Žižak, Željko
AU  - Dojčinović, Biljana
AU  - Stanković, Dalibor M.
AU  - Antić, Bratislav
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10733
AB  - Partial cation substitution can significantly change the physical properties of parent compounds. By controlling the chemical composition and knowing the mutual relationship between composition and physical properties, it is possible to tailor the properties of materials to those that are superior for desired technological application. Using the polyol synthesis procedure, a series of yttrium-substituted iron oxide nanoconstructs, γ-Fe2−xYxO3 (YIONs), was prepared. It was found that Y3+ could substitute Fe3+ in the crystal structures of maghemite (γ-Fe2O3) up to a limited concentration of ~1.5% (γ-Fe1.969Y0.031O3). Analysis of TEM micrographs showed that crystallites or particles were aggregated in flower-like structures with diameters from 53.7 ± 6.2 nm to 97.3 ± 37.0 nm, depending on yttrium concentration. To be investigated for potential applications as magnetic hyperthermia agents, YIONs were tested twice: their heating efficiency was tested and their toxicity was investigated. The Specific Absorption Rate (SAR) values were in the range of 32.6 W/g to 513 W/g and significantly decreased with increased yttrium concentration in the samples. Intrinsic loss power (ILP) for γ-Fe2O3 and γ-Fe1.995Y0.005O3 were ~8–9 nH·m2/Kg, which pointed to their excellent heating efficiency. IC50 values of investigated samples against cancer (HeLa) and normal (MRC-5) cells decreased with increased yttrium concentration and were higher than ~300 μg/mL. The samples of γ-Fe2−xYxO3 did not show a genotoxic effect. The results of toxicity studies show that YIONs are suitable for further in vitro/in vivo studies toward to their potential medical applications, while results of heat generation point to their potential use in magnetic hyperthermia cancer treatment or use as self-heating systems for other technological applications such as catalysis.
T2  - Nanomaterials
T1  - Self-Heating Flower-like Nanoconstructs with Limited Incorporation of Yttrium in Maghemite: Effect of Chemical Composition on Heating Efficiency, Cytotoxicity and Genotoxicity
VL  - 13
IS  - 5
SP  - 870
DO  - 10.3390/nano13050870
ER  - 

@article{
author = "Ognjanović, Miloš and Jaćimović, Željko and Kosović-Perutović, Milica and Besu Žižak, Irina and Stanojković, Tatjana and Žižak, Željko and Dojčinović, Biljana and Stanković, Dalibor M. and Antić, Bratislav",
year = "2023",
abstract = "Partial cation substitution can significantly change the physical properties of parent compounds. By controlling the chemical composition and knowing the mutual relationship between composition and physical properties, it is possible to tailor the properties of materials to those that are superior for desired technological application. Using the polyol synthesis procedure, a series of yttrium-substituted iron oxide nanoconstructs, γ-Fe2−xYxO3 (YIONs), was prepared. It was found that Y3+ could substitute Fe3+ in the crystal structures of maghemite (γ-Fe2O3) up to a limited concentration of ~1.5% (γ-Fe1.969Y0.031O3). Analysis of TEM micrographs showed that crystallites or particles were aggregated in flower-like structures with diameters from 53.7 ± 6.2 nm to 97.3 ± 37.0 nm, depending on yttrium concentration. To be investigated for potential applications as magnetic hyperthermia agents, YIONs were tested twice: their heating efficiency was tested and their toxicity was investigated. The Specific Absorption Rate (SAR) values were in the range of 32.6 W/g to 513 W/g and significantly decreased with increased yttrium concentration in the samples. Intrinsic loss power (ILP) for γ-Fe2O3 and γ-Fe1.995Y0.005O3 were ~8–9 nH·m2/Kg, which pointed to their excellent heating efficiency. IC50 values of investigated samples against cancer (HeLa) and normal (MRC-5) cells decreased with increased yttrium concentration and were higher than ~300 μg/mL. The samples of γ-Fe2−xYxO3 did not show a genotoxic effect. The results of toxicity studies show that YIONs are suitable for further in vitro/in vivo studies toward to their potential medical applications, while results of heat generation point to their potential use in magnetic hyperthermia cancer treatment or use as self-heating systems for other technological applications such as catalysis.",
journal = "Nanomaterials",
title = "Self-Heating Flower-like Nanoconstructs with Limited Incorporation of Yttrium in Maghemite: Effect of Chemical Composition on Heating Efficiency, Cytotoxicity and Genotoxicity",
volume = "13",
number = "5",
pages = "870",
doi = "10.3390/nano13050870"
}

Ognjanović, M., Jaćimović, Ž., Kosović-Perutović, M., Besu Žižak, I., Stanojković, T., Žižak, Ž., Dojčinović, B., Stanković, D. M.,& Antić, B.. (2023). Self-Heating Flower-like Nanoconstructs with Limited Incorporation of Yttrium in Maghemite: Effect of Chemical Composition on Heating Efficiency, Cytotoxicity and Genotoxicity. in Nanomaterials, 13(5), 870.
https://doi.org/10.3390/nano13050870

Ognjanović M, Jaćimović Ž, Kosović-Perutović M, Besu Žižak I, Stanojković T, Žižak Ž, Dojčinović B, Stanković DM, Antić B. Self-Heating Flower-like Nanoconstructs with Limited Incorporation of Yttrium in Maghemite: Effect of Chemical Composition on Heating Efficiency, Cytotoxicity and Genotoxicity. in Nanomaterials. 2023;13(5):870.
doi:10.3390/nano13050870 .

Ognjanović, Miloš, Jaćimović, Željko, Kosović-Perutović, Milica, Besu Žižak, Irina, Stanojković, Tatjana, Žižak, Željko, Dojčinović, Biljana, Stanković, Dalibor M., Antić, Bratislav, "Self-Heating Flower-like Nanoconstructs with Limited Incorporation of Yttrium in Maghemite: Effect of Chemical Composition on Heating Efficiency, Cytotoxicity and Genotoxicity" in Nanomaterials, 13, no. 5 (2023):870,
https://doi.org/10.3390/nano13050870 . .

TY  - CONF
AU  - Ognjanović, Miloš
AU  - Stanojković, Tatjana
AU  - Dojčinović, Biljana
AU  - Radović, Magdalena
AU  - Mirković, Marija
AU  - Vranješ-Đurić, Sanja
AU  - Antić, Bratislav
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11672
AB  - A series of MgxFe3-xO4 (x=0, 0.1, 0.2, 0.4, 0.6, 0.8, and 1) magnetic nanoparticles (MNP) were synthesized by a two-step procedure, a co-precipitation method followed by hydrothermal treatment in a microwave field. The MNP are single-core, with crystallite size gradually decreasing from 15.5(3) up to 2.5(3) nm with an increase ofx. TEM images show pseudospherical log-normally distributed particles with an average particle diameter of 19.8 nm and a polydispersity index of 26.1% for magnetite. The particle diameter decreases with the increase of magnesium (x) in the formula unit. The colloidal stability of MNP was achieved by their surface modification with citric acid (CA), oleic acid (OA) and polyethylene glycol (PEG). The cytotoxic activity of uncoated and coated Mg0.6Fe2.4O4 was tested against target malignant cells (HeLa, LC174, A549) and normal MRC5 cells. The investigated MNP show moderate cytotoxic activity against the tested malignant cells in vitro. In contrast, MNP didn’tshow any significant cytotoxic effect against normal cells. HeLa cells exhibited the highest susceptibility among the malignant cells. Mg0.6Fe2.4O4@OA show good cytotoxic activity against all examined malignant cells, significantly higher than other tested MNP. It can be seen that Mg0.6Fe2.4O4@PEG show a lower cytotoxic activity compared to all analyzed MNP. A direct method was used for labeling with radionuclide 90Y, which involves incubation of MNP with 90Y at a certain temperature and time. The labeling yield of the 90Y-coated MNP was determined by analyzing the radiochemical purity after labeling. 90YMg0.2Fe2.8O4@PEG were labeled in high yield (100%), while the yield for 90YMg0.2Fe2.8O4@CA was 83%. In vitro stability of 90Y-coated MNP at room temperature in physiological solution and human serum was monitored within 72 h from the moment of labeling by determining the radiochemical purity of ITLC-SG by radio chromatographic method. The stability of 90Y-Mg0.2Fe2.8O4@PEG was about 97%, while 90Y-Mg0.2Fe2.8O4@CA stability was 73%. The results of this study indicate that radiolabeled surface-modified (Mg, Fe)3O4 can be used as vectors in radionuclide therapy of malignant diseases.
PB  - Society of Chemists and Technologists of Macedonia
C3  - 26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia
T1  - Radiolabeled surface-modified single-core (Mg,Fe)3O4 colloidal nanoparticles as vectors in radionuclidetherapy of cancer
SP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11672
ER  - 

@conference{
author = "Ognjanović, Miloš and Stanojković, Tatjana and Dojčinović, Biljana and Radović, Magdalena and Mirković, Marija and Vranješ-Đurić, Sanja and Antić, Bratislav",
year = "2023",
abstract = "A series of MgxFe3-xO4 (x=0, 0.1, 0.2, 0.4, 0.6, 0.8, and 1) magnetic nanoparticles (MNP) were synthesized by a two-step procedure, a co-precipitation method followed by hydrothermal treatment in a microwave field. The MNP are single-core, with crystallite size gradually decreasing from 15.5(3) up to 2.5(3) nm with an increase ofx. TEM images show pseudospherical log-normally distributed particles with an average particle diameter of 19.8 nm and a polydispersity index of 26.1% for magnetite. The particle diameter decreases with the increase of magnesium (x) in the formula unit. The colloidal stability of MNP was achieved by their surface modification with citric acid (CA), oleic acid (OA) and polyethylene glycol (PEG). The cytotoxic activity of uncoated and coated Mg0.6Fe2.4O4 was tested against target malignant cells (HeLa, LC174, A549) and normal MRC5 cells. The investigated MNP show moderate cytotoxic activity against the tested malignant cells in vitro. In contrast, MNP didn’tshow any significant cytotoxic effect against normal cells. HeLa cells exhibited the highest susceptibility among the malignant cells. Mg0.6Fe2.4O4@OA show good cytotoxic activity against all examined malignant cells, significantly higher than other tested MNP. It can be seen that Mg0.6Fe2.4O4@PEG show a lower cytotoxic activity compared to all analyzed MNP. A direct method was used for labeling with radionuclide 90Y, which involves incubation of MNP with 90Y at a certain temperature and time. The labeling yield of the 90Y-coated MNP was determined by analyzing the radiochemical purity after labeling. 90YMg0.2Fe2.8O4@PEG were labeled in high yield (100%), while the yield for 90YMg0.2Fe2.8O4@CA was 83%. In vitro stability of 90Y-coated MNP at room temperature in physiological solution and human serum was monitored within 72 h from the moment of labeling by determining the radiochemical purity of ITLC-SG by radio chromatographic method. The stability of 90Y-Mg0.2Fe2.8O4@PEG was about 97%, while 90Y-Mg0.2Fe2.8O4@CA stability was 73%. The results of this study indicate that radiolabeled surface-modified (Mg, Fe)3O4 can be used as vectors in radionuclide therapy of malignant diseases.",
publisher = "Society of Chemists and Technologists of Macedonia",
journal = "26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia",
title = "Radiolabeled surface-modified single-core (Mg,Fe)3O4 colloidal nanoparticles as vectors in radionuclidetherapy of cancer",
pages = "186",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11672"
}

Ognjanović, M., Stanojković, T., Dojčinović, B., Radović, M., Mirković, M., Vranješ-Đurić, S.,& Antić, B.. (2023). Radiolabeled surface-modified single-core (Mg,Fe)3O4 colloidal nanoparticles as vectors in radionuclidetherapy of cancer. in 26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia
Society of Chemists and Technologists of Macedonia., 186.
https://hdl.handle.net/21.15107/rcub_vinar_11672

Ognjanović M, Stanojković T, Dojčinović B, Radović M, Mirković M, Vranješ-Đurić S, Antić B. Radiolabeled surface-modified single-core (Mg,Fe)3O4 colloidal nanoparticles as vectors in radionuclidetherapy of cancer. in 26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia. 2023;:186.
https://hdl.handle.net/21.15107/rcub_vinar_11672 .

Ognjanović, Miloš, Stanojković, Tatjana, Dojčinović, Biljana, Radović, Magdalena, Mirković, Marija, Vranješ-Đurić, Sanja, Antić, Bratislav, "Radiolabeled surface-modified single-core (Mg,Fe)3O4 colloidal nanoparticles as vectors in radionuclidetherapy of cancer" in 26th Congress of the Society of Chemists and Technologists of Macedonia : the book of abstracts; September 20-23, Ohrid, Macedonia (2023):186,
https://hdl.handle.net/21.15107/rcub_vinar_11672 .

TY  - CONF
AU  - Pašić, Ivana
AU  - Preljević, Kenan
AU  - Matić, Ivana
AU  - Petrović, Nina
AU  - Stanojković, Tatjana
AU  - Perović, Svetlana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12566
AB  - Background: It has been demonstrated that aromaƟ c plant essenƟ al oils (EO) contain phytochemicals with anƟ - infl ammatory, anƟ oxidant, and anƟ cancer acƟ viƟ es. The goal of this study was to examine the cytotoxicity and the anƟ cancer acƟ on mechanisms of an EO extracted from Satureja montana plant species, originaƟ ng from Montenegro. Material and methods: The cytotoxic acƟ vity was assessed against human cancer cell lines: cervical adenocarcinoma HeLa, malignant melanoma A375, colorectal adenocarcinoma LS 174T, lung carcinoma A549, as well as against normal human lung fi broblasts MRC-5 by MTT assay. Using fl ow cytometry, it was examined how HeLa cells were distributed throughout the cell cycle aŌ er treatment with EO and whether caspase-3, caspase-8, and caspase-9 were acƟ vated. RT-qPCR was used to assess the expression levels of genes and miRNA in HeLa cells. Results: Satureja montana EO exerted strong cytotoxicity against human cancer cell lines, with IC50 values ranging from 0,12 to 0,18 µL/mL, The strongest cytotoxic acƟ vity of EO was observed against lung carcinoma A549 cells with an IC50 value of 0.12 µL/mL and LS 174T colorectal adenocarcinoma cells with IC50 value of 0.13 µL/mL. The lowest cytotoxicity was determined against normal lung fi broblasts MRC-5 (IC50 value of 0.19 µL/mL). AŌ er 24 hours of treatment with Satureja montana EO, a striking increase in the proporƟ on of HeLa cells in the subG1 phase was seen in comparison with control cells. Pretreatment of HeLa cells with caspase inhibitors showed that Satureja montana EO acƟ vated all three invesƟ gated caspases to trigger apoptosis. In comparison to control cells, Satureja montana EO treatment of HeLa cells decreased MMP2 gene expression levels, and elevated MMP9 and VEGFA gene expression levels. Satureja montana EO treatment increased the expression levels of the tumor supressive miR-16 and miR-34ain HeLa cells, and increased levels of miR21, with oncogenic role in cervical cancer. Conclusion: Satureja montana EO showed strong cytotoxic eff ects on tested cancer cells. EO exerted prominent apoptoƟ c acƟ vity in HeLa cells via intrinsic and extrinsic pathways. Decrease in the expression levels of MMP2 gene, involved in invasion and metastasis, and cancer suppressive miRNAs, further support its potenƟ al to be uƟ lized as a therapeuƟ c agent in cancer treatment.
PB  - Belgrade : Serbian Association for Cancer Research
C3  - Oncology Insights
T1  - Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro
IS  - 1
SP  - 67
EP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12566
ER  - 

@conference{
author = "Pašić, Ivana and Preljević, Kenan and Matić, Ivana and Petrović, Nina and Stanojković, Tatjana and Perović, Svetlana",
year = "2023",
abstract = "Background: It has been demonstrated that aromaƟ c plant essenƟ al oils (EO) contain phytochemicals with anƟ - infl ammatory, anƟ oxidant, and anƟ cancer acƟ viƟ es. The goal of this study was to examine the cytotoxicity and the anƟ cancer acƟ on mechanisms of an EO extracted from Satureja montana plant species, originaƟ ng from Montenegro. Material and methods: The cytotoxic acƟ vity was assessed against human cancer cell lines: cervical adenocarcinoma HeLa, malignant melanoma A375, colorectal adenocarcinoma LS 174T, lung carcinoma A549, as well as against normal human lung fi broblasts MRC-5 by MTT assay. Using fl ow cytometry, it was examined how HeLa cells were distributed throughout the cell cycle aŌ er treatment with EO and whether caspase-3, caspase-8, and caspase-9 were acƟ vated. RT-qPCR was used to assess the expression levels of genes and miRNA in HeLa cells. Results: Satureja montana EO exerted strong cytotoxicity against human cancer cell lines, with IC50 values ranging from 0,12 to 0,18 µL/mL, The strongest cytotoxic acƟ vity of EO was observed against lung carcinoma A549 cells with an IC50 value of 0.12 µL/mL and LS 174T colorectal adenocarcinoma cells with IC50 value of 0.13 µL/mL. The lowest cytotoxicity was determined against normal lung fi broblasts MRC-5 (IC50 value of 0.19 µL/mL). AŌ er 24 hours of treatment with Satureja montana EO, a striking increase in the proporƟ on of HeLa cells in the subG1 phase was seen in comparison with control cells. Pretreatment of HeLa cells with caspase inhibitors showed that Satureja montana EO acƟ vated all three invesƟ gated caspases to trigger apoptosis. In comparison to control cells, Satureja montana EO treatment of HeLa cells decreased MMP2 gene expression levels, and elevated MMP9 and VEGFA gene expression levels. Satureja montana EO treatment increased the expression levels of the tumor supressive miR-16 and miR-34ain HeLa cells, and increased levels of miR21, with oncogenic role in cervical cancer. Conclusion: Satureja montana EO showed strong cytotoxic eff ects on tested cancer cells. EO exerted prominent apoptoƟ c acƟ vity in HeLa cells via intrinsic and extrinsic pathways. Decrease in the expression levels of MMP2 gene, involved in invasion and metastasis, and cancer suppressive miRNAs, further support its potenƟ al to be uƟ lized as a therapeuƟ c agent in cancer treatment.",
publisher = "Belgrade : Serbian Association for Cancer Research",
journal = "Oncology Insights",
title = "Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro",
number = "1",
pages = "67-68",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12566"
}

Pašić, I., Preljević, K., Matić, I., Petrović, N., Stanojković, T.,& Perović, S.. (2023). Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro. in Oncology Insights
Belgrade : Serbian Association for Cancer Research.(1), 67-68.
https://hdl.handle.net/21.15107/rcub_vinar_12566

Pašić I, Preljević K, Matić I, Petrović N, Stanojković T, Perović S. Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro. in Oncology Insights. 2023;(1):67-68.
https://hdl.handle.net/21.15107/rcub_vinar_12566 .

Pašić, Ivana, Preljević, Kenan, Matić, Ivana, Petrović, Nina, Stanojković, Tatjana, Perović, Svetlana, "Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro" in Oncology Insights, no. 1 (2023):67-68,
https://hdl.handle.net/21.15107/rcub_vinar_12566 .

TY  - JOUR
AU  - Marković, Maja D.
AU  - Tadić, Julijana D.
AU  - Savić, Sanja I.
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana P.
AU  - Mijin, Dušan Ž.
AU  - Panić, Vesna V.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10262
AB  - Researchers are faced with everyday demands for safer and more efficient therapy for many diseases, especially serious one such as various types of cancer. Numerous anticancer drugs are poorly-water soluble and therefore their encapsulation and controlled release remain quite challenge. In present study, we deepened our research of hydrophilic carrier based on poly(methacrylic acid) and casein (PMAC) by investigating its potential for encapsulation and controlled release of novel poorly water-soluble dihydropyrimidion-azo-pyridon compound (DHPMP). DHPMP is a dye that has been proven to show cytotoxic activity against chronic myeloid leukemia K562 cells. By encapsulating DHPMP into the carrier and delivering it into the intestines, DHPMP absorption could be the fastest and the number of therapeutic doses and side effects can be reduced. Carriers based on PMAC and DHPMP (PMAC-DHPMP) were synthetized and characterized by FTIR, SEM and single compression tests. The swelling behavior of PMAC-DHPMP carriers and cumulative DHPMP release were investigated depending on the amount of crosslinker and encapsulated DHPMP in two media which were simulating pH environments in human stomach and intestines. The prolonged and controlled release of DHPMP was achieved. In vitro cytotoxic activity of PMAC-DHPMP carriers against K562 cells and the cell cycle analysis showed great potential of the carriers for application in leukemia treatment.
T2  - Journal of Biomedical Materials Research - Part A
T1  - Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment
DO  - 10.1002/jbm.a.37396
ER  - 

@article{
author = "Marković, Maja D. and Tadić, Julijana D. and Savić, Sanja I. and Matić, Ivana Z. and Stanojković, Tatjana P. and Mijin, Dušan Ž. and Panić, Vesna V.",
year = "2022",
abstract = "Researchers are faced with everyday demands for safer and more efficient therapy for many diseases, especially serious one such as various types of cancer. Numerous anticancer drugs are poorly-water soluble and therefore their encapsulation and controlled release remain quite challenge. In present study, we deepened our research of hydrophilic carrier based on poly(methacrylic acid) and casein (PMAC) by investigating its potential for encapsulation and controlled release of novel poorly water-soluble dihydropyrimidion-azo-pyridon compound (DHPMP). DHPMP is a dye that has been proven to show cytotoxic activity against chronic myeloid leukemia K562 cells. By encapsulating DHPMP into the carrier and delivering it into the intestines, DHPMP absorption could be the fastest and the number of therapeutic doses and side effects can be reduced. Carriers based on PMAC and DHPMP (PMAC-DHPMP) were synthetized and characterized by FTIR, SEM and single compression tests. The swelling behavior of PMAC-DHPMP carriers and cumulative DHPMP release were investigated depending on the amount of crosslinker and encapsulated DHPMP in two media which were simulating pH environments in human stomach and intestines. The prolonged and controlled release of DHPMP was achieved. In vitro cytotoxic activity of PMAC-DHPMP carriers against K562 cells and the cell cycle analysis showed great potential of the carriers for application in leukemia treatment.",
journal = "Journal of Biomedical Materials Research - Part A",
title = "Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment",
doi = "10.1002/jbm.a.37396"
}

Marković, M. D., Tadić, J. D., Savić, S. I., Matić, I. Z., Stanojković, T. P., Mijin, D. Ž.,& Panić, V. V.. (2022). Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment. in Journal of Biomedical Materials Research - Part A.
https://doi.org/10.1002/jbm.a.37396

Marković MD, Tadić JD, Savić SI, Matić IZ, Stanojković TP, Mijin DŽ, Panić VV. Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment. in Journal of Biomedical Materials Research - Part A. 2022;.
doi:10.1002/jbm.a.37396 .

Marković, Maja D., Tadić, Julijana D., Savić, Sanja I., Matić, Ivana Z., Stanojković, Tatjana P., Mijin, Dušan Ž., Panić, Vesna V., "Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment" in Journal of Biomedical Materials Research - Part A (2022),
https://doi.org/10.1002/jbm.a.37396 . .

TY  - JOUR
AU  - Stepanović, Aleksandar
AU  - Nikitović, Marina
AU  - Stanojković, Tatjana P.
AU  - Grujičić, Danica
AU  - Bukumirić, Zoran
AU  - Srbljak, Ivana
AU  - Ilić, Rosanda
AU  - Milošević, Snežana
AU  - Arsenijević, Tatjana
AU  - Petrović, Nina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10265
AB  - A personalized approach to chemoradiation is important in reducing its potential side effects and identifying a group of patients prone to toxicity. MicroRNAs have been shown to have a predictive potential for radiotoxicity. The goal of the study was to test if levels of miRNA in peripheral blood mononuclear cells of glioblastoma patients are associated with toxicity and to identify the peak time point for toxicity. MicroRNA-10b/21/34a levels were measured in 43 patients with and without toxicity, at baseline, at the 15th, and at the 30th fraction by Real-Time quantitative Polymerase Chain Reaction. MicroRNA-10b/21 levels increased with toxicity grade (p = 0.014; p = 0.013); miR-21/34a levels were significantly different between patients with and without toxicity at the 15th fraction (p = 0.030; p = 0.045), while miR-34a levels significantly changed during treatment (p < 0.001). All three miRNAs showed a significantly high positive correlation with one another. MiR-34a might be considered as a predictive factor for toxicity due to its changes during treatment, and differences between the groups with and without toxicity; miR-10b might be used to predict toxicity; miR-10b/21 might be used for predicting the grade of toxicity in GB patients.
T2  - Scientific Reports
T1  - Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide
VL  - 12
IS  - 1
SP  - 7505
DO  - 10.1038/s41598-022-11445-9
ER  - 

@article{
author = "Stepanović, Aleksandar and Nikitović, Marina and Stanojković, Tatjana P. and Grujičić, Danica and Bukumirić, Zoran and Srbljak, Ivana and Ilić, Rosanda and Milošević, Snežana and Arsenijević, Tatjana and Petrović, Nina",
year = "2022",
abstract = "A personalized approach to chemoradiation is important in reducing its potential side effects and identifying a group of patients prone to toxicity. MicroRNAs have been shown to have a predictive potential for radiotoxicity. The goal of the study was to test if levels of miRNA in peripheral blood mononuclear cells of glioblastoma patients are associated with toxicity and to identify the peak time point for toxicity. MicroRNA-10b/21/34a levels were measured in 43 patients with and without toxicity, at baseline, at the 15th, and at the 30th fraction by Real-Time quantitative Polymerase Chain Reaction. MicroRNA-10b/21 levels increased with toxicity grade (p = 0.014; p = 0.013); miR-21/34a levels were significantly different between patients with and without toxicity at the 15th fraction (p = 0.030; p = 0.045), while miR-34a levels significantly changed during treatment (p < 0.001). All three miRNAs showed a significantly high positive correlation with one another. MiR-34a might be considered as a predictive factor for toxicity due to its changes during treatment, and differences between the groups with and without toxicity; miR-10b might be used to predict toxicity; miR-10b/21 might be used for predicting the grade of toxicity in GB patients.",
journal = "Scientific Reports",
title = "Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide",
volume = "12",
number = "1",
pages = "7505",
doi = "10.1038/s41598-022-11445-9"
}

Stepanović, A., Nikitović, M., Stanojković, T. P., Grujičić, D., Bukumirić, Z., Srbljak, I., Ilić, R., Milošević, S., Arsenijević, T.,& Petrović, N.. (2022). Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide. in Scientific Reports, 12(1), 7505.
https://doi.org/10.1038/s41598-022-11445-9

Stepanović A, Nikitović M, Stanojković TP, Grujičić D, Bukumirić Z, Srbljak I, Ilić R, Milošević S, Arsenijević T, Petrović N. Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide. in Scientific Reports. 2022;12(1):7505.
doi:10.1038/s41598-022-11445-9 .

Stepanović, Aleksandar, Nikitović, Marina, Stanojković, Tatjana P., Grujičić, Danica, Bukumirić, Zoran, Srbljak, Ivana, Ilić, Rosanda, Milošević, Snežana, Arsenijević, Tatjana, Petrović, Nina, "Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide" in Scientific Reports, 12, no. 1 (2022):7505,
https://doi.org/10.1038/s41598-022-11445-9 . .

TY  - JOUR
AU  - Mališić, Emina
AU  - Petrović, Nina
AU  - Brengues, Muriel
AU  - Azria, David
AU  - Matić, Ivana Z.
AU  - Srbljak Ćuk, Ivana
AU  - Kopčalić, Katarina
AU  - Stanojković, Tatjana P.
AU  - Nikitović, Marina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10544
AB  - The genetic background of each person might affect the severity of radiotherapy (RT)-induced normal tissue toxicity. The aim of study was to evaluate the influence of TGFB1 C-509T and Leu10Pro, XRCC1 Arg280His and XRCC3 Thr241Met polymorphisms as well as the level of radiation-induced CD8 T-lymphocyte apoptosis (RILA) on adverse effects of RT for prostate cancer (PCa). The study included 88 patients with localized or locally advanced PCa who were treated with RT. The polymorphisms were determined by PCR–RFLP analysis on DNA from peripheral blood mononuclear cells. RILA values were measured by flow cytometry. We found that CT genotype of TGFB1 C-509T could be protective biomarker for acute genitourinary (GU) and gastrointestinal (GI) radiotoxicity, while Thr variant of XRCC3 Thr241Met could predict the risk for acute GU radiotoxicity. Correlation between RILA values and toxicity was not detected. Univariate logistic regression analysis showed that Gleason score and risk group were risk factors for late GU, while for late GI radiotoxicity it was diabetes mellitus type 2. However, in multivariate model those were not proven to be significant and independent risk factors. Identification of assays combination predicting individual radiosensitivity is a crucial step towards personalized RT approach.
T2  - Scientific Reports
T1  - Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer
VL  - 12
IS  - 1
SP  - 21306
DO  - 10.1038/s41598-022-25328-6
ER  - 

@article{
author = "Mališić, Emina and Petrović, Nina and Brengues, Muriel and Azria, David and Matić, Ivana Z. and Srbljak Ćuk, Ivana and Kopčalić, Katarina and Stanojković, Tatjana P. and Nikitović, Marina",
year = "2022",
abstract = "The genetic background of each person might affect the severity of radiotherapy (RT)-induced normal tissue toxicity. The aim of study was to evaluate the influence of TGFB1 C-509T and Leu10Pro, XRCC1 Arg280His and XRCC3 Thr241Met polymorphisms as well as the level of radiation-induced CD8 T-lymphocyte apoptosis (RILA) on adverse effects of RT for prostate cancer (PCa). The study included 88 patients with localized or locally advanced PCa who were treated with RT. The polymorphisms were determined by PCR–RFLP analysis on DNA from peripheral blood mononuclear cells. RILA values were measured by flow cytometry. We found that CT genotype of TGFB1 C-509T could be protective biomarker for acute genitourinary (GU) and gastrointestinal (GI) radiotoxicity, while Thr variant of XRCC3 Thr241Met could predict the risk for acute GU radiotoxicity. Correlation between RILA values and toxicity was not detected. Univariate logistic regression analysis showed that Gleason score and risk group were risk factors for late GU, while for late GI radiotoxicity it was diabetes mellitus type 2. However, in multivariate model those were not proven to be significant and independent risk factors. Identification of assays combination predicting individual radiosensitivity is a crucial step towards personalized RT approach.",
journal = "Scientific Reports",
title = "Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer",
volume = "12",
number = "1",
pages = "21306",
doi = "10.1038/s41598-022-25328-6"
}

Mališić, E., Petrović, N., Brengues, M., Azria, D., Matić, I. Z., Srbljak Ćuk, I., Kopčalić, K., Stanojković, T. P.,& Nikitović, M.. (2022). Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer. in Scientific Reports, 12(1), 21306.
https://doi.org/10.1038/s41598-022-25328-6

Mališić E, Petrović N, Brengues M, Azria D, Matić IZ, Srbljak Ćuk I, Kopčalić K, Stanojković TP, Nikitović M. Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer. in Scientific Reports. 2022;12(1):21306.
doi:10.1038/s41598-022-25328-6 .

Mališić, Emina, Petrović, Nina, Brengues, Muriel, Azria, David, Matić, Ivana Z., Srbljak Ćuk, Ivana, Kopčalić, Katarina, Stanojković, Tatjana P., Nikitović, Marina, "Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer" in Scientific Reports, 12, no. 1 (2022):21306,
https://doi.org/10.1038/s41598-022-25328-6 . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Ergün, Sercan
AU  - Đorđić-Crnogorac, Marija
AU  - Stanojković, Tatjana
AU  - Mališić, Emina
AU  - Matić Ivana Z.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10706
AB  - Phytochemicals and bioactive substances derived from a wide range of plant extracts have been reported to exert various anticancer effects. Prostate cancer is one of the leading causes of cancer-related deaths within the male population. Prostate cancer-specific miRNA signatures were associated with cancer formation and progression, with various subtypes, and response to therapy. MicroRNA levels of expression were shown to change after the treatment of various compounds and substances extracted from natural products. Natural herbal compounds were shown to induce variations in miRNA expression levels in cancer cells. The aims of this study were to investigate the cytotoxic effects of methanol, ethyl-acetate, and hexane extracts obtained from branch-body part and flowers of Hypericum perforatum L. against humane PC-3 and DU 145 and to test potential miRNA-128/133b/155/193a/206/21/335 signature changes and differences between the two prostate cancer cell lines. Cytotoxic activity of H. perforatum extracts, their effects on cell cycle distribution, and miRNA expression levels were examined in humane PC-3 and DU 145 prostate cancer cells by MTT cell survival assay, flow cytometry, and quantitative real-time PCR. Hexane extract of flowers showed the strongest intensity of cytotoxic activity against PC-3 and DU 145 cells. The highest increase in the percentage of PC-3 cells in the subG1 phase was observed in cell samples treated with hexane extract of flowers and branch-body part. Significant differences in miRNA-128/133b/155/193a/206/21/335 levels were observed between PC-3 and DU 145 cell lines, especially in samples treated with flower extracts compared with the branch-body part. Conclusions: Investigated extracts have significant anticancer potential not only from the aspects of cytotoxicity and cell cycle effects but also from the aspect of lowering oncogenic or increasing tumor-suppressive miRNAs. The best effect might be the increase of tumor-suppressive miR-128 (accompanied by miR-193a) induced by the hexane extract of the flowers, which also exerted the highest cytotoxic activity. Hexane extract of flowers may be the candidate for further investigation for improving the efficiency of standard therapies for PCa. A miRNA signature might be cell-type specific after the treatment with H. perforatum extracts.
T2  - Genetika
T1  - Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells
VL  - 54
IS  - 3
SP  - 1249
EP  - 1270
DO  - 10.2298/GENSR2203249P
ER  - 

@article{
author = "Petrović, Nina and Ergün, Sercan and Đorđić-Crnogorac, Marija and Stanojković, Tatjana and Mališić, Emina and Matić Ivana Z.",
year = "2022",
abstract = "Phytochemicals and bioactive substances derived from a wide range of plant extracts have been reported to exert various anticancer effects. Prostate cancer is one of the leading causes of cancer-related deaths within the male population. Prostate cancer-specific miRNA signatures were associated with cancer formation and progression, with various subtypes, and response to therapy. MicroRNA levels of expression were shown to change after the treatment of various compounds and substances extracted from natural products. Natural herbal compounds were shown to induce variations in miRNA expression levels in cancer cells. The aims of this study were to investigate the cytotoxic effects of methanol, ethyl-acetate, and hexane extracts obtained from branch-body part and flowers of Hypericum perforatum L. against humane PC-3 and DU 145 and to test potential miRNA-128/133b/155/193a/206/21/335 signature changes and differences between the two prostate cancer cell lines. Cytotoxic activity of H. perforatum extracts, their effects on cell cycle distribution, and miRNA expression levels were examined in humane PC-3 and DU 145 prostate cancer cells by MTT cell survival assay, flow cytometry, and quantitative real-time PCR. Hexane extract of flowers showed the strongest intensity of cytotoxic activity against PC-3 and DU 145 cells. The highest increase in the percentage of PC-3 cells in the subG1 phase was observed in cell samples treated with hexane extract of flowers and branch-body part. Significant differences in miRNA-128/133b/155/193a/206/21/335 levels were observed between PC-3 and DU 145 cell lines, especially in samples treated with flower extracts compared with the branch-body part. Conclusions: Investigated extracts have significant anticancer potential not only from the aspects of cytotoxicity and cell cycle effects but also from the aspect of lowering oncogenic or increasing tumor-suppressive miRNAs. The best effect might be the increase of tumor-suppressive miR-128 (accompanied by miR-193a) induced by the hexane extract of the flowers, which also exerted the highest cytotoxic activity. Hexane extract of flowers may be the candidate for further investigation for improving the efficiency of standard therapies for PCa. A miRNA signature might be cell-type specific after the treatment with H. perforatum extracts.",
journal = "Genetika",
title = "Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells",
volume = "54",
number = "3",
pages = "1249-1270",
doi = "10.2298/GENSR2203249P"
}

Petrović, N., Ergün, S., Đorđić-Crnogorac, M., Stanojković, T., Mališić, E.,& Matić Ivana Z.. (2022). Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells. in Genetika, 54(3), 1249-1270.
https://doi.org/10.2298/GENSR2203249P

Petrović N, Ergün S, Đorđić-Crnogorac M, Stanojković T, Mališić E, Matić Ivana Z.. Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells. in Genetika. 2022;54(3):1249-1270.
doi:10.2298/GENSR2203249P .

Petrović, Nina, Ergün, Sercan, Đorđić-Crnogorac, Marija, Stanojković, Tatjana, Mališić, Emina, Matić Ivana Z., "Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells" in Genetika, 54, no. 3 (2022):1249-1270,
https://doi.org/10.2298/GENSR2203249P . .

TY  - CONF
AU  - Pašić, Ivana
AU  - Srbljak Ćuk, Ivana
AU  - Petrović, Nina
AU  - Matić, Ivana
AU  - Džudžević-Čančar, Hurija
AU  - Dedić, Alema
AU  - Alispahić, Amra
AU  - Boškailo, Emina
AU  - Stanojković, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12300
AB  - Lavandula angustifolia Mill. (lavender) is an aromatic and medicinal herb whose flower essential oils (EO) are widely used for the treatment of gastrointestinal, nervous, and rheumatic disorders, and in the perfume industry. Laurus nobilis L. (laurel bay) is an evergreen tree whose EOs have antimicrobial and anti-inflammatory effects. Lavender and bay were collected from Sarajevo and Mostar in Bosnia and Herzegovina. The extraction was performed by hydrodistillation in Clevenger-type apparatus. Phytochemical composition was analyzed by gas chromatography coupled with mass spectrometry. Cytotoxic activities of lavender EO and bay leaf, fruit and seed EOs were investigated against human cervical adenocarcinoma HeLa cells and non-transformed human lung fibroblasts MRC-5 by MTT cell survival assay. Cell cycle phase distribution was examined by flow cytometry. In bay EOs the most abundant component was 1,8-cineole, followed by linalool, bicyclic monoterpenes sabinene, αpinene, and β-pinene. Components identified in the fruit and seed, but not in the leaf were (E)-β-ocymene, camphene, β-elemene, bornyl acetate and trans-caryophyllene. The major component of lavender extract was linalool accompanied by linalyl acetate, lavandulyl acetate, camphor, 1,8-cneole, borneol, α-terpineol, and terpinene-4-ol. The four tested EOs showed concentration-dependent cytotoxic effects on HeLa and MRC-5 cells. Among examined EOs, lavender EO exerted the strongest cytotoxic activity on HeLa cells with IC50 value of 0.11 µL/mL. Bay seed and fruit EOs exerted stronger cytotoxicity on HeLa cells than bay leaf EO (IC50 values: 0.17, 0.21, and 3.35 µL/mL, respectively). When compared with sensitivity of HeLa cells, normal MRC-5 cells showed similar sensitivity to the cytotoxic activity of the four tested EOs. Lavender EO applied at IC50 concentration, during 24 h caused remarkable increase in the percentage of HeLa cells within the subG1 cell cycle phase, in comparison with control cells (64.69% vs 2.47%). Pretreatment with caspase-3, caspase-8 or caspase-9 inhibitor before 24 h treatment with lavender EO did not cause changes in the percentage of cells in the subG1 phase in comparison with HeLa cells exposed only to lavender oil. Our results showed that lavender and bay EOs exerted potent cytotoxic activity against HeLa cells. Additional investigations are necessary to explore cytotoxic effects of these EOs against various cancer cell lines and mechanisms underlying anticancer effects.
PB  - Zagreb : Croatian Association for Cancer Research
C3  - 6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts
T1  - Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells
SP  - 43
EP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12300
ER  - 

@conference{
author = "Pašić, Ivana and Srbljak Ćuk, Ivana and Petrović, Nina and Matić, Ivana and Džudžević-Čančar, Hurija and Dedić, Alema and Alispahić, Amra and Boškailo, Emina and Stanojković, Tatjana",
year = "2022",
abstract = "Lavandula angustifolia Mill. (lavender) is an aromatic and medicinal herb whose flower essential oils (EO) are widely used for the treatment of gastrointestinal, nervous, and rheumatic disorders, and in the perfume industry. Laurus nobilis L. (laurel bay) is an evergreen tree whose EOs have antimicrobial and anti-inflammatory effects. Lavender and bay were collected from Sarajevo and Mostar in Bosnia and Herzegovina. The extraction was performed by hydrodistillation in Clevenger-type apparatus. Phytochemical composition was analyzed by gas chromatography coupled with mass spectrometry. Cytotoxic activities of lavender EO and bay leaf, fruit and seed EOs were investigated against human cervical adenocarcinoma HeLa cells and non-transformed human lung fibroblasts MRC-5 by MTT cell survival assay. Cell cycle phase distribution was examined by flow cytometry. In bay EOs the most abundant component was 1,8-cineole, followed by linalool, bicyclic monoterpenes sabinene, αpinene, and β-pinene. Components identified in the fruit and seed, but not in the leaf were (E)-β-ocymene, camphene, β-elemene, bornyl acetate and trans-caryophyllene. The major component of lavender extract was linalool accompanied by linalyl acetate, lavandulyl acetate, camphor, 1,8-cneole, borneol, α-terpineol, and terpinene-4-ol. The four tested EOs showed concentration-dependent cytotoxic effects on HeLa and MRC-5 cells. Among examined EOs, lavender EO exerted the strongest cytotoxic activity on HeLa cells with IC50 value of 0.11 µL/mL. Bay seed and fruit EOs exerted stronger cytotoxicity on HeLa cells than bay leaf EO (IC50 values: 0.17, 0.21, and 3.35 µL/mL, respectively). When compared with sensitivity of HeLa cells, normal MRC-5 cells showed similar sensitivity to the cytotoxic activity of the four tested EOs. Lavender EO applied at IC50 concentration, during 24 h caused remarkable increase in the percentage of HeLa cells within the subG1 cell cycle phase, in comparison with control cells (64.69% vs 2.47%). Pretreatment with caspase-3, caspase-8 or caspase-9 inhibitor before 24 h treatment with lavender EO did not cause changes in the percentage of cells in the subG1 phase in comparison with HeLa cells exposed only to lavender oil. Our results showed that lavender and bay EOs exerted potent cytotoxic activity against HeLa cells. Additional investigations are necessary to explore cytotoxic effects of these EOs against various cancer cell lines and mechanisms underlying anticancer effects.",
publisher = "Zagreb : Croatian Association for Cancer Research",
journal = "6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts",
title = "Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells",
pages = "43-43",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12300"
}

Pašić, I., Srbljak Ćuk, I., Petrović, N., Matić, I., Džudžević-Čančar, H., Dedić, A., Alispahić, A., Boškailo, E.,& Stanojković, T.. (2022). Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells. in 6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts
Zagreb : Croatian Association for Cancer Research., 43-43.
https://hdl.handle.net/21.15107/rcub_vinar_12300

Pašić I, Srbljak Ćuk I, Petrović N, Matić I, Džudžević-Čančar H, Dedić A, Alispahić A, Boškailo E, Stanojković T. Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells. in 6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts. 2022;:43-43.
https://hdl.handle.net/21.15107/rcub_vinar_12300 .

Pašić, Ivana, Srbljak Ćuk, Ivana, Petrović, Nina, Matić, Ivana, Džudžević-Čančar, Hurija, Dedić, Alema, Alispahić, Amra, Boškailo, Emina, Stanojković, Tatjana, "Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells" in 6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts (2022):43-43,
https://hdl.handle.net/21.15107/rcub_vinar_12300 .

TY  - JOUR
AU  - Petronijević, Jelena
AU  - Joksimović, Nenad
AU  - Milović, Emilija
AU  - Đorđić Crnogorac, Marija
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Milivojević, Dušan
AU  - Janković, Nenad
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9933
AB  - In order to discover new therapeutically active agents a series of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones were synthesized. All complexes were characterized by IR and EPR spectroscopic techniques and examined for their cytotoxic effect on human cancer cell lines HeLa, LS174, A549 and normal fibroblasts (MRC-5). For further examination of the cytotoxic mechanisms of novel complexes, three of them were chosen for analysing their effects on the distribution of HeLa cells in the cell cycle phases. The results of the flow cytometry analysis suggest that tested complexes lead to time-dependent accumulation of the cells in S and G2/M phases. The strongest accumulation effect showed complex 2d after 48 h of incubation. Competitive experiments with ethidium bromide (EB) indicated that tested compound 2d have affinity to displace EB from the EB-DNA complex through intercalation. Also, the binding parameters values for 2d-BSA complex showed that a reversible 2d-BSA complex is formed and ligand 2d can be stored and carried by BSA.
T2  - Chemico-Biological Interactions
T1  - Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones
VL  - 348
SP  - 109647
DO  - 10.1016/j.cbi.2021.109647
ER  - 

@article{
author = "Petronijević, Jelena and Joksimović, Nenad and Milović, Emilija and Đorđić Crnogorac, Marija and Petrović, Nina and Stanojković, Tatjana P. and Milivojević, Dušan and Janković, Nenad",
year = "2021",
abstract = "In order to discover new therapeutically active agents a series of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones were synthesized. All complexes were characterized by IR and EPR spectroscopic techniques and examined for their cytotoxic effect on human cancer cell lines HeLa, LS174, A549 and normal fibroblasts (MRC-5). For further examination of the cytotoxic mechanisms of novel complexes, three of them were chosen for analysing their effects on the distribution of HeLa cells in the cell cycle phases. The results of the flow cytometry analysis suggest that tested complexes lead to time-dependent accumulation of the cells in S and G2/M phases. The strongest accumulation effect showed complex 2d after 48 h of incubation. Competitive experiments with ethidium bromide (EB) indicated that tested compound 2d have affinity to displace EB from the EB-DNA complex through intercalation. Also, the binding parameters values for 2d-BSA complex showed that a reversible 2d-BSA complex is formed and ligand 2d can be stored and carried by BSA.",
journal = "Chemico-Biological Interactions",
title = "Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones",
volume = "348",
pages = "109647",
doi = "10.1016/j.cbi.2021.109647"
}

Petronijević, J., Joksimović, N., Milović, E., Đorđić Crnogorac, M., Petrović, N., Stanojković, T. P., Milivojević, D.,& Janković, N.. (2021). Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones. in Chemico-Biological Interactions, 348, 109647.
https://doi.org/10.1016/j.cbi.2021.109647

Petronijević J, Joksimović N, Milović E, Đorđić Crnogorac M, Petrović N, Stanojković TP, Milivojević D, Janković N. Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones. in Chemico-Biological Interactions. 2021;348:109647.
doi:10.1016/j.cbi.2021.109647 .

Petronijević, Jelena, Joksimović, Nenad, Milović, Emilija, Đorđić Crnogorac, Marija, Petrović, Nina, Stanojković, Tatjana P., Milivojević, Dušan, Janković, Nenad, "Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones" in Chemico-Biological Interactions, 348 (2021):109647,
https://doi.org/10.1016/j.cbi.2021.109647 . .

TY  - JOUR
AU  - Tadić, Julijana D.
AU  - Lađarević, Jelena M.
AU  - Vitnik, Željko J.
AU  - Vitnik, Vesna D.
AU  - Stanojković, Tatjana P.
AU  - Matić, Ivana Z.
AU  - Mijin, Dušan Ž.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10890
AB  - Seven novel azo dyes with 2-pyridone and dihydropyrimidinone moieties have been synthesized and thoroughly characterized. The azo-hydrazone tautomerism has been investigated by experimental and theoretical approaches. The optimizations of geometries have been performed with density functional theory (DFT). The vibrational and NMR spectra were calculated and correlated with experimental ones. Furthermore, quantum chemical descriptors were calculated and MEP maps were plotted to determine biological reactivity of dyes. The antioxidant assay evinced that 5, 6 and 7 are promising antioxidant candidates. In vitro cytotoxic activity was studied against three malignant cell lines: prostate adenocarcinoma (PC-3), lung carcinoma (A549) and chronic myelogenous leukemia (K562), as well as against human normal lung fibroblasts (MRC-5), using MTT assay. Examination of cytotoxic effects on human cancer cell lines showed the concentration dependent cytotoxicity of all investigated compounds. The K562 cells were the most sensitive to the cytotoxicity of the compounds 3, 5 and 6, wherein compound 5 was particularly prominent and selective in cytotoxic action between K562 (24.97 μM) and PC-3 (48.98 μM) cancer cells, and normal MRC-5 (91.11 μM) cells. Moreover, the cell cycle analysis of compound 5 was examined in K562 cells, by flow cytometry, to study its mechanism of anticancer action. Finally, in silico evaluation of physicochemical parameters, druglikeness and ADME properties showed that investigated compounds are orally bioavailable with no permeation to the blood brain barrier.
T2  - Dyes and Pigments
T1  - Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity
VL  - 187
SP  - 109123
DO  - 10.1016/j.dyepig.2020.109123
ER  - 

@article{
author = "Tadić, Julijana D. and Lađarević, Jelena M. and Vitnik, Željko J. and Vitnik, Vesna D. and Stanojković, Tatjana P. and Matić, Ivana Z. and Mijin, Dušan Ž.",
year = "2021",
abstract = "Seven novel azo dyes with 2-pyridone and dihydropyrimidinone moieties have been synthesized and thoroughly characterized. The azo-hydrazone tautomerism has been investigated by experimental and theoretical approaches. The optimizations of geometries have been performed with density functional theory (DFT). The vibrational and NMR spectra were calculated and correlated with experimental ones. Furthermore, quantum chemical descriptors were calculated and MEP maps were plotted to determine biological reactivity of dyes. The antioxidant assay evinced that 5, 6 and 7 are promising antioxidant candidates. In vitro cytotoxic activity was studied against three malignant cell lines: prostate adenocarcinoma (PC-3), lung carcinoma (A549) and chronic myelogenous leukemia (K562), as well as against human normal lung fibroblasts (MRC-5), using MTT assay. Examination of cytotoxic effects on human cancer cell lines showed the concentration dependent cytotoxicity of all investigated compounds. The K562 cells were the most sensitive to the cytotoxicity of the compounds 3, 5 and 6, wherein compound 5 was particularly prominent and selective in cytotoxic action between K562 (24.97 μM) and PC-3 (48.98 μM) cancer cells, and normal MRC-5 (91.11 μM) cells. Moreover, the cell cycle analysis of compound 5 was examined in K562 cells, by flow cytometry, to study its mechanism of anticancer action. Finally, in silico evaluation of physicochemical parameters, druglikeness and ADME properties showed that investigated compounds are orally bioavailable with no permeation to the blood brain barrier.",
journal = "Dyes and Pigments",
title = "Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity",
volume = "187",
pages = "109123",
doi = "10.1016/j.dyepig.2020.109123"
}

Tadić, J. D., Lađarević, J. M., Vitnik, Ž. J., Vitnik, V. D., Stanojković, T. P., Matić, I. Z.,& Mijin, D. Ž.. (2021). Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity. in Dyes and Pigments, 187, 109123.
https://doi.org/10.1016/j.dyepig.2020.109123

Tadić JD, Lađarević JM, Vitnik ŽJ, Vitnik VD, Stanojković TP, Matić IZ, Mijin DŽ. Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity. in Dyes and Pigments. 2021;187:109123.
doi:10.1016/j.dyepig.2020.109123 .

Tadić, Julijana D., Lađarević, Jelena M., Vitnik, Željko J., Vitnik, Vesna D., Stanojković, Tatjana P., Matić, Ivana Z., Mijin, Dušan Ž., "Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity" in Dyes and Pigments, 187 (2021):109123,
https://doi.org/10.1016/j.dyepig.2020.109123 . .

TY  - JOUR
AU  - Matić, Ivana Z.
AU  - Ergün, Sercan
AU  - Đorđić Crnogorac, Marija
AU  - Misir, Sema
AU  - Aliyazicioğlu, Yüksel
AU  - Damjanović, Ana
AU  - Džudžević-Čančar, Hurija
AU  - Stanojković, Tatjana P.
AU  - Konanç, Kalbiye
AU  - Petrović, Nina
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9406
AB  - Six extracts were obtained from plant species Hypericum perforatum L., collected at Samsun in Turkey. The aim of this study was to examine the mechanisms of the anticancer activity of these extracts. Methanol, ethyl-acetate and hexane were used as a solvents for extraction from both branch-body part of the plant (extracts 1, 2 and 3) and from plant flowers (extracts 4, 5 and 6). The cytotoxic effects of the extracts were determined against 2D and 3D cancer cell models. Cell cycle changes of treated HeLa cells were analyzed by flow cytometry. Measurements of gene and microRNA expression levels in treated HeLa cells were done by quantitative real time PCR. Five examined extracts (2–6) exerted selective concentration-dependent cytotoxic effects on HeLa, K562, and A549 cancer cells, while the extract 1 exhibited very weak cytotoxicity. The extract 6 showed the highest intensity of cytotoxic activity. All tested extracts (2–6) demonstrated the ability to induce apoptosis in HeLa cells through activation of caspase-3. These extracts remarkably decreased gene expression levels of MMP2, MMP9, TIMP3, and VEGFA in HeLa cells. Flower extracts might have stronger effects on miR128/193a-5p/335 level changes than branch-body extracts. Hypericum perforatum extracts exerted weaker cytotoxic effects on 3D HeLa spheroids when compared with their effects on 2D monolayer HeLa cells. Taken together, results of our research may suggest the promising anticancer properties of the Hypericum perforatum extracts. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
T2  - Cytotechnology
T1  - Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models
VL  - 73
IS  - 3
SP  - 373
EP  - 389
DO  - 10.1007/s10616-021-00464-5
ER  - 

@article{
author = "Matić, Ivana Z. and Ergün, Sercan and Đorđić Crnogorac, Marija and Misir, Sema and Aliyazicioğlu, Yüksel and Damjanović, Ana and Džudžević-Čančar, Hurija and Stanojković, Tatjana P. and Konanç, Kalbiye and Petrović, Nina",
year = "2021",
abstract = "Six extracts were obtained from plant species Hypericum perforatum L., collected at Samsun in Turkey. The aim of this study was to examine the mechanisms of the anticancer activity of these extracts. Methanol, ethyl-acetate and hexane were used as a solvents for extraction from both branch-body part of the plant (extracts 1, 2 and 3) and from plant flowers (extracts 4, 5 and 6). The cytotoxic effects of the extracts were determined against 2D and 3D cancer cell models. Cell cycle changes of treated HeLa cells were analyzed by flow cytometry. Measurements of gene and microRNA expression levels in treated HeLa cells were done by quantitative real time PCR. Five examined extracts (2–6) exerted selective concentration-dependent cytotoxic effects on HeLa, K562, and A549 cancer cells, while the extract 1 exhibited very weak cytotoxicity. The extract 6 showed the highest intensity of cytotoxic activity. All tested extracts (2–6) demonstrated the ability to induce apoptosis in HeLa cells through activation of caspase-3. These extracts remarkably decreased gene expression levels of MMP2, MMP9, TIMP3, and VEGFA in HeLa cells. Flower extracts might have stronger effects on miR128/193a-5p/335 level changes than branch-body extracts. Hypericum perforatum extracts exerted weaker cytotoxic effects on 3D HeLa spheroids when compared with their effects on 2D monolayer HeLa cells. Taken together, results of our research may suggest the promising anticancer properties of the Hypericum perforatum extracts. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.",
journal = "Cytotechnology",
title = "Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models",
volume = "73",
number = "3",
pages = "373-389",
doi = "10.1007/s10616-021-00464-5"
}

Matić, I. Z., Ergün, S., Đorđić Crnogorac, M., Misir, S., Aliyazicioğlu, Y., Damjanović, A., Džudžević-Čančar, H., Stanojković, T. P., Konanç, K.,& Petrović, N.. (2021). Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models. in Cytotechnology, 73(3), 373-389.
https://doi.org/10.1007/s10616-021-00464-5

Matić IZ, Ergün S, Đorđić Crnogorac M, Misir S, Aliyazicioğlu Y, Damjanović A, Džudžević-Čančar H, Stanojković TP, Konanç K, Petrović N. Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models. in Cytotechnology. 2021;73(3):373-389.
doi:10.1007/s10616-021-00464-5 .

Matić, Ivana Z., Ergün, Sercan, Đorđić Crnogorac, Marija, Misir, Sema, Aliyazicioğlu, Yüksel, Damjanović, Ana, Džudžević-Čančar, Hurija, Stanojković, Tatjana P., Konanç, Kalbiye, Petrović, Nina, "Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models" in Cytotechnology, 73, no. 3 (2021):373-389,
https://doi.org/10.1007/s10616-021-00464-5 . .

TY  - JOUR
AU  - Stanojković, Tatjana P.
AU  - Matić, Ivana Z.
AU  - Petrović, Nina
AU  - Stanković, Vesna
AU  - Kopčalić, Katarina
AU  - Besu, Irina
AU  - Đorđić Crnogorac, Marija
AU  - Mališić, Emina
AU  - Mirjačić-Martinović, Katarina
AU  - Vuletić, Ana
AU  - Bukumirić, Zoran
AU  - Žižak, Željko
AU  - Veldwijk, Marlon
AU  - Herskind, Carsten
AU  - Nikitović, Marina
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9713
AB  - One of the challenges of radiation oncology in the era of personalized medicine is identification of biomarkers associated with individual radiosensitivity. The aim of research was to evaluate the possible clinical value of the associations between clinical, physical, and biological factors, and risk for development of acute radiotoxicity in patients with prostate cancer. The study involved forty four patients treated with three-dimensional conformal radiotherapy. The concentrations of IL-1β, IL-2, IL-6, IFN-γ and TGF-β1 were assessed before radiotherapy, after 5th, 15th and 25th radiotherapy fractions, at the end, and 1 month after the end of radiotherapy. Cytokine gene expression was determined in peripheral blood mononuclear cells. The univariate analysis of circulating cytokine levels during radiotherapy showed that increased serum concentrations of IL-6 were significantly associated with higher grade of acute genitourinary toxicity. The multivariate analysis demonstrated that increased level of IL-6 during the radiotherapy was significantly associated with higher grade of acute genitourinary toxicity across treatment. TGF-β expression levels significantly decreased during course of radiotherapy. Research indicates that changes in circulating cytokine levels might be important parameter of radiotoxicity in patients with prostate cancer. These findings suggest that future studies based on multi-parameter examination are necessary for prediction of individual radiosensitivity.
T2  - Scientific Reports
T1  - Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients
VL  - 10
IS  - 1
SP  - 19002
DO  - 10.1038/s41598-020-75812-0
ER  - 

@article{
author = "Stanojković, Tatjana P. and Matić, Ivana Z. and Petrović, Nina and Stanković, Vesna and Kopčalić, Katarina and Besu, Irina and Đorđić Crnogorac, Marija and Mališić, Emina and Mirjačić-Martinović, Katarina and Vuletić, Ana and Bukumirić, Zoran and Žižak, Željko and Veldwijk, Marlon and Herskind, Carsten and Nikitović, Marina",
year = "2020",
abstract = "One of the challenges of radiation oncology in the era of personalized medicine is identification of biomarkers associated with individual radiosensitivity. The aim of research was to evaluate the possible clinical value of the associations between clinical, physical, and biological factors, and risk for development of acute radiotoxicity in patients with prostate cancer. The study involved forty four patients treated with three-dimensional conformal radiotherapy. The concentrations of IL-1β, IL-2, IL-6, IFN-γ and TGF-β1 were assessed before radiotherapy, after 5th, 15th and 25th radiotherapy fractions, at the end, and 1 month after the end of radiotherapy. Cytokine gene expression was determined in peripheral blood mononuclear cells. The univariate analysis of circulating cytokine levels during radiotherapy showed that increased serum concentrations of IL-6 were significantly associated with higher grade of acute genitourinary toxicity. The multivariate analysis demonstrated that increased level of IL-6 during the radiotherapy was significantly associated with higher grade of acute genitourinary toxicity across treatment. TGF-β expression levels significantly decreased during course of radiotherapy. Research indicates that changes in circulating cytokine levels might be important parameter of radiotoxicity in patients with prostate cancer. These findings suggest that future studies based on multi-parameter examination are necessary for prediction of individual radiosensitivity.",
journal = "Scientific Reports",
title = "Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients",
volume = "10",
number = "1",
pages = "19002",
doi = "10.1038/s41598-020-75812-0"
}

Stanojković, T. P., Matić, I. Z., Petrović, N., Stanković, V., Kopčalić, K., Besu, I., Đorđić Crnogorac, M., Mališić, E., Mirjačić-Martinović, K., Vuletić, A., Bukumirić, Z., Žižak, Ž., Veldwijk, M., Herskind, C.,& Nikitović, M.. (2020). Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients. in Scientific Reports, 10(1), 19002.
https://doi.org/10.1038/s41598-020-75812-0

Stanojković TP, Matić IZ, Petrović N, Stanković V, Kopčalić K, Besu I, Đorđić Crnogorac M, Mališić E, Mirjačić-Martinović K, Vuletić A, Bukumirić Z, Žižak Ž, Veldwijk M, Herskind C, Nikitović M. Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients. in Scientific Reports. 2020;10(1):19002.
doi:10.1038/s41598-020-75812-0 .

Stanojković, Tatjana P., Matić, Ivana Z., Petrović, Nina, Stanković, Vesna, Kopčalić, Katarina, Besu, Irina, Đorđić Crnogorac, Marija, Mališić, Emina, Mirjačić-Martinović, Katarina, Vuletić, Ana, Bukumirić, Zoran, Žižak, Željko, Veldwijk, Marlon, Herskind, Carsten, Nikitović, Marina, "Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients" in Scientific Reports, 10, no. 1 (2020):19002,
https://doi.org/10.1038/s41598-020-75812-0 . .

TY  - JOUR
AU  - Janković, Nenad Ž.
AU  - Trifunović Ristovski, Jovana
AU  - Vraneš, Milan
AU  - Tot, Aleksandar
AU  - Petronijević, Jelena
AU  - Joksimović, Nenad
AU  - Stanojković, Tatjana P.
AU  - Đorđić Crnogorac, Marija
AU  - Petrović, Nina
AU  - Boljević, Ivana
AU  - Matić, Ivana Z.
AU  - Bogdanović, Goran A.
AU  - Mikov, Momir
AU  - Bugarčić, Zorica M.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0045206818312598
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8071
AB  - In order to investigate potential therapeutically agents, novel products of Biginelli reaction (4a-l) were synthesized and exposed to cytotoxic and caspase activities, angiogenesis, cell cycle distribution, gene and microRNA expression levels, lipophilicity assessment and docking study. Among the twelve novel compounds (4a-l) evaluated for the cytotoxic activity, five of them (4c, 4d, 4f, 4k and 4l) that showed excellent activity on the tested cell lines (HeLa, LS174 and A549) were selected for further evaluation. Interestingly, compound 4f has up to three times higher selectivity index (SI) towards cancer cells than cisplatin (on HeLa, LS174 and A549 SI = 18.2, 13.5 and 11.2, respectively). The obtained results from cell cycle distribution and caspase activity indicate that tested compounds (4c, 4d, 4f, 4k and 4l) promoted caspase-9 activation, implicated in the intrinsic pathway of apoptosis. Lipophilicity of 4a-l was determinate by using reversed-phase high-performance liquid chromatography. © 2019 Elsevier Inc.
T2  - Bioorganic Chemistry
T1  - Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels
VL  - 86
SP  - 569
EP  - 582
DO  - 10.1016/j.bioorg.2019.02.026
ER  - 

@article{
author = "Janković, Nenad Ž. and Trifunović Ristovski, Jovana and Vraneš, Milan and Tot, Aleksandar and Petronijević, Jelena and Joksimović, Nenad and Stanojković, Tatjana P. and Đorđić Crnogorac, Marija and Petrović, Nina and Boljević, Ivana and Matić, Ivana Z. and Bogdanović, Goran A. and Mikov, Momir and Bugarčić, Zorica M.",
year = "2019",
abstract = "In order to investigate potential therapeutically agents, novel products of Biginelli reaction (4a-l) were synthesized and exposed to cytotoxic and caspase activities, angiogenesis, cell cycle distribution, gene and microRNA expression levels, lipophilicity assessment and docking study. Among the twelve novel compounds (4a-l) evaluated for the cytotoxic activity, five of them (4c, 4d, 4f, 4k and 4l) that showed excellent activity on the tested cell lines (HeLa, LS174 and A549) were selected for further evaluation. Interestingly, compound 4f has up to three times higher selectivity index (SI) towards cancer cells than cisplatin (on HeLa, LS174 and A549 SI = 18.2, 13.5 and 11.2, respectively). The obtained results from cell cycle distribution and caspase activity indicate that tested compounds (4c, 4d, 4f, 4k and 4l) promoted caspase-9 activation, implicated in the intrinsic pathway of apoptosis. Lipophilicity of 4a-l was determinate by using reversed-phase high-performance liquid chromatography. © 2019 Elsevier Inc.",
journal = "Bioorganic Chemistry",
title = "Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels",
volume = "86",
pages = "569-582",
doi = "10.1016/j.bioorg.2019.02.026"
}

Janković, N. Ž., Trifunović Ristovski, J., Vraneš, M., Tot, A., Petronijević, J., Joksimović, N., Stanojković, T. P., Đorđić Crnogorac, M., Petrović, N., Boljević, I., Matić, I. Z., Bogdanović, G. A., Mikov, M.,& Bugarčić, Z. M.. (2019). Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels. in Bioorganic Chemistry, 86, 569-582.
https://doi.org/10.1016/j.bioorg.2019.02.026

Janković NŽ, Trifunović Ristovski J, Vraneš M, Tot A, Petronijević J, Joksimović N, Stanojković TP, Đorđić Crnogorac M, Petrović N, Boljević I, Matić IZ, Bogdanović GA, Mikov M, Bugarčić ZM. Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels. in Bioorganic Chemistry. 2019;86:569-582.
doi:10.1016/j.bioorg.2019.02.026 .

Janković, Nenad Ž., Trifunović Ristovski, Jovana, Vraneš, Milan, Tot, Aleksandar, Petronijević, Jelena, Joksimović, Nenad, Stanojković, Tatjana P., Đorđić Crnogorac, Marija, Petrović, Nina, Boljević, Ivana, Matić, Ivana Z., Bogdanović, Goran A., Mikov, Momir, Bugarčić, Zorica M., "Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels" in Bioorganic Chemistry, 86 (2019):569-582,
https://doi.org/10.1016/j.bioorg.2019.02.026 . .

TY  - JOUR
AU  - Kopčalić, Katarina
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Stanković, Vesna
AU  - Bukumirić, Zoran
AU  - Roganović, Jelena
AU  - Mališić, Emina
AU  - Nikitović, Marina
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8444
AB  - Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs. © 2018 Elsevier GmbH
T2  - Pathology - Research and Practice
T1  - Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study
VL  - 215
IS  - 4
SP  - 626
EP  - 631
DO  - 10.1016/j.prp.2018.12.007
ER  - 

@article{
author = "Kopčalić, Katarina and Petrović, Nina and Stanojković, Tatjana P. and Stanković, Vesna and Bukumirić, Zoran and Roganović, Jelena and Mališić, Emina and Nikitović, Marina",
year = "2019",
abstract = "Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs. © 2018 Elsevier GmbH",
journal = "Pathology - Research and Practice",
title = "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study",
volume = "215",
number = "4",
pages = "626-631",
doi = "10.1016/j.prp.2018.12.007"
}

Kopčalić, K., Petrović, N., Stanojković, T. P., Stanković, V., Bukumirić, Z., Roganović, J., Mališić, E.,& Nikitović, M.. (2019). Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology - Research and Practice, 215(4), 626-631.
https://doi.org/10.1016/j.prp.2018.12.007

Kopčalić K, Petrović N, Stanojković TP, Stanković V, Bukumirić Z, Roganović J, Mališić E, Nikitović M. Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology - Research and Practice. 2019;215(4):626-631.
doi:10.1016/j.prp.2018.12.007 .

Kopčalić, Katarina, Petrović, Nina, Stanojković, Tatjana P., Stanković, Vesna, Bukumirić, Zoran, Roganović, Jelena, Mališić, Emina, Nikitović, Marina, "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study" in Pathology - Research and Practice, 215, no. 4 (2019):626-631,
https://doi.org/10.1016/j.prp.2018.12.007 . .

TY  - JOUR
AU  - Jakovljević, Katarina
AU  - Joksović, Milan D.
AU  - Matić, Ivana Z.
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Sladić, Dušan M.
AU  - Vujčić, Miroslava T.
AU  - Janović, Barbara S.
AU  - Joksović, Ljubinka G.
AU  - Trifunović, Snežana
AU  - Marković, Violeta
PY  - 2018
UR  - http://xlink.rsc.org/?DOI=C8MD00316E
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7928
AB  - Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.
T2  - MedChemComm
T1  - Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies
VL  - 9
IS  - 10
SP  - 1679
EP  - 1697
DO  - 10.1039/C8MD00316E
ER  - 

@article{
author = "Jakovljević, Katarina and Joksović, Milan D. and Matić, Ivana Z. and Petrović, Nina and Stanojković, Tatjana P. and Sladić, Dušan M. and Vujčić, Miroslava T. and Janović, Barbara S. and Joksović, Ljubinka G. and Trifunović, Snežana and Marković, Violeta",
year = "2018",
abstract = "Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.",
journal = "MedChemComm",
title = "Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies",
volume = "9",
number = "10",
pages = "1679-1697",
doi = "10.1039/C8MD00316E"
}

Jakovljević, K., Joksović, M. D., Matić, I. Z., Petrović, N., Stanojković, T. P., Sladić, D. M., Vujčić, M. T., Janović, B. S., Joksović, L. G., Trifunović, S.,& Marković, V.. (2018). Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in MedChemComm, 9(10), 1679-1697.
https://doi.org/10.1039/C8MD00316E

Jakovljević K, Joksović MD, Matić IZ, Petrović N, Stanojković TP, Sladić DM, Vujčić MT, Janović BS, Joksović LG, Trifunović S, Marković V. Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in MedChemComm. 2018;9(10):1679-1697.
doi:10.1039/C8MD00316E .

Jakovljević, Katarina, Joksović, Milan D., Matić, Ivana Z., Petrović, Nina, Stanojković, Tatjana P., Sladić, Dušan M., Vujčić, Miroslava T., Janović, Barbara S., Joksović, Ljubinka G., Trifunović, Snežana, Marković, Violeta, "Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies" in MedChemComm, 9, no. 10 (2018):1679-1697,
https://doi.org/10.1039/C8MD00316E . .

TY  - JOUR
AU  - Stanojković, Tatjana P.
AU  - Marković, Violeta
AU  - Matić, Ivana Z.
AU  - Mladenović, Milan P.
AU  - Petrović, Nina
AU  - Krivokuća, Ana M.
AU  - Petković, Miloš R.
AU  - Joksović, Milan D.
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0960894X18305493
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7815
AB  - A series of 23 novel anthraquinone-chalcone hybrids containing amide function was synthesized and structurally characterized. Sixteen compounds exerted strong cytotoxic activities against K562, Jurkat and HL-60 leukemia cell lines and significantly lower cytotoxic effects against normal MRC-5 cells, indicating very high selectivity in their anticancer action. The compounds 6g, 6u and 6v activate apoptosis in K562 cells through the extrinsic and intrinsic apoptotic pathway. The compound 6e triggered apoptosis in K562 cells only through the extrinsic apoptotic pathway. Treatment of K562 cells with each of these four compounds caused decrease in the expression levels of MMP2, MMP9, and VEGF, suggesting their anti-invasive, antimetastatic and antiangiogenic properties. The compounds 6g and 6v downregulated expression levels of miR-155 in K562 cells, while compounds 6e and 6u upregulated miR-155 levels in treated cells, in comparison with control cells. The structure-based 3-D QSAR models for 6f, 6e, 6i and 6l describe pro-apoptotic activity against caspase-3. © 2018 Elsevier Ltd
T2  - Bioorganic and Medicinal Chemistry Letters
T1  - Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents
VL  - 28
IS  - 15
SP  - 2593
EP  - 2598
DO  - 10.1016/j.bmcl.2018.06.048
ER  - 

@article{
author = "Stanojković, Tatjana P. and Marković, Violeta and Matić, Ivana Z. and Mladenović, Milan P. and Petrović, Nina and Krivokuća, Ana M. and Petković, Miloš R. and Joksović, Milan D.",
year = "2018",
abstract = "A series of 23 novel anthraquinone-chalcone hybrids containing amide function was synthesized and structurally characterized. Sixteen compounds exerted strong cytotoxic activities against K562, Jurkat and HL-60 leukemia cell lines and significantly lower cytotoxic effects against normal MRC-5 cells, indicating very high selectivity in their anticancer action. The compounds 6g, 6u and 6v activate apoptosis in K562 cells through the extrinsic and intrinsic apoptotic pathway. The compound 6e triggered apoptosis in K562 cells only through the extrinsic apoptotic pathway. Treatment of K562 cells with each of these four compounds caused decrease in the expression levels of MMP2, MMP9, and VEGF, suggesting their anti-invasive, antimetastatic and antiangiogenic properties. The compounds 6g and 6v downregulated expression levels of miR-155 in K562 cells, while compounds 6e and 6u upregulated miR-155 levels in treated cells, in comparison with control cells. The structure-based 3-D QSAR models for 6f, 6e, 6i and 6l describe pro-apoptotic activity against caspase-3. © 2018 Elsevier Ltd",
journal = "Bioorganic and Medicinal Chemistry Letters",
title = "Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents",
volume = "28",
number = "15",
pages = "2593-2598",
doi = "10.1016/j.bmcl.2018.06.048"
}

Stanojković, T. P., Marković, V., Matić, I. Z., Mladenović, M. P., Petrović, N., Krivokuća, A. M., Petković, M. R.,& Joksović, M. D.. (2018). Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents. in Bioorganic and Medicinal Chemistry Letters, 28(15), 2593-2598.
https://doi.org/10.1016/j.bmcl.2018.06.048

Stanojković TP, Marković V, Matić IZ, Mladenović MP, Petrović N, Krivokuća AM, Petković MR, Joksović MD. Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents. in Bioorganic and Medicinal Chemistry Letters. 2018;28(15):2593-2598.
doi:10.1016/j.bmcl.2018.06.048 .

Stanojković, Tatjana P., Marković, Violeta, Matić, Ivana Z., Mladenović, Milan P., Petrović, Nina, Krivokuća, Ana M., Petković, Miloš R., Joksović, Milan D., "Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents" in Bioorganic and Medicinal Chemistry Letters, 28, no. 15 (2018):2593-2598,
https://doi.org/10.1016/j.bmcl.2018.06.048 . .

TY  - JOUR
AU  - Burmudžija, Adrijana Z.
AU  - Muškinja, Jovana
AU  - Kosanić, Marijana
AU  - Ranković, Branislav
AU  - Novaković, Slađana B.
AU  - Đorđević, Snežana
AU  - Stanojković, Tatjana P.
AU  - Baskić, Dejan D.
AU  - Ratković, Zoran R.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1680
AB  - A small series of 1-acetyl-2-(4-alkoxy-3-methoxyphenyl)cyclopropanes was prepared, starting from dehydrozingerone (4-(4-hydroxy-3-methoxyphenyl)-3-buten-2-one) and its O-alkyl derivatives. Their microbiological activities toward some strains of bacteria and fungi were tested, as well as their in vitro cytotoxic activity against some cancer cell lines (HeLa, LS174 and A549). All synthesized compounds showed significant antimicrobial activity and expressed cytotoxic activity against tested carcinoma cell lines, but they showed no significant influence on normal cell line (MRC5). Butyl derivative is the most active on HeLa cells (IC50 = 8.63 m), while benzyl one is active against LS174 and A549 cell lines (IC50 = 10.17 and 12.15 m, respectively).
T2  - Chemistry and Biodiversity
T1  - Cytotoxic and Antimicrobial Activity of Dehydrozingerone based Cyclopropyl Derivatives
VL  - 14
IS  - 8
DO  - 10.1002/cbdv.201700077
ER  - 

@article{
author = "Burmudžija, Adrijana Z. and Muškinja, Jovana and Kosanić, Marijana and Ranković, Branislav and Novaković, Slađana B. and Đorđević, Snežana and Stanojković, Tatjana P. and Baskić, Dejan D. and Ratković, Zoran R.",
year = "2017",
abstract = "A small series of 1-acetyl-2-(4-alkoxy-3-methoxyphenyl)cyclopropanes was prepared, starting from dehydrozingerone (4-(4-hydroxy-3-methoxyphenyl)-3-buten-2-one) and its O-alkyl derivatives. Their microbiological activities toward some strains of bacteria and fungi were tested, as well as their in vitro cytotoxic activity against some cancer cell lines (HeLa, LS174 and A549). All synthesized compounds showed significant antimicrobial activity and expressed cytotoxic activity against tested carcinoma cell lines, but they showed no significant influence on normal cell line (MRC5). Butyl derivative is the most active on HeLa cells (IC50 = 8.63 m), while benzyl one is active against LS174 and A549 cell lines (IC50 = 10.17 and 12.15 m, respectively).",
journal = "Chemistry and Biodiversity",
title = "Cytotoxic and Antimicrobial Activity of Dehydrozingerone based Cyclopropyl Derivatives",
volume = "14",
number = "8",
doi = "10.1002/cbdv.201700077"
}

Burmudžija, A. Z., Muškinja, J., Kosanić, M., Ranković, B., Novaković, S. B., Đorđević, S., Stanojković, T. P., Baskić, D. D.,& Ratković, Z. R.. (2017). Cytotoxic and Antimicrobial Activity of Dehydrozingerone based Cyclopropyl Derivatives. in Chemistry and Biodiversity, 14(8).
https://doi.org/10.1002/cbdv.201700077

Burmudžija AZ, Muškinja J, Kosanić M, Ranković B, Novaković SB, Đorđević S, Stanojković TP, Baskić DD, Ratković ZR. Cytotoxic and Antimicrobial Activity of Dehydrozingerone based Cyclopropyl Derivatives. in Chemistry and Biodiversity. 2017;14(8).
doi:10.1002/cbdv.201700077 .

Burmudžija, Adrijana Z., Muškinja, Jovana, Kosanić, Marijana, Ranković, Branislav, Novaković, Slađana B., Đorđević, Snežana, Stanojković, Tatjana P., Baskić, Dejan D., Ratković, Zoran R., "Cytotoxic and Antimicrobial Activity of Dehydrozingerone based Cyclopropyl Derivatives" in Chemistry and Biodiversity, 14, no. 8 (2017),
https://doi.org/10.1002/cbdv.201700077 . .

TY  - JOUR
AU  - Rodić, Marko V.
AU  - Leovac, Vukadin M.
AU  - Jovanović, Ljiljana S.
AU  - Spasojević, Vojislav
AU  - Joksović, Milan D.
AU  - Stanojković, Tatjana P.
AU  - Matić, Ivana Z.
AU  - Vojinović-Ješić, Ljiljana S.
AU  - Marković, Violeta
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1053
AB  - Three novel copper complexes with tridentate N2O ligand di(2-pyridil) ketone 1-adamantoyl hydrazone (Addpy) of the formula [(Cu2Cu2I)-Cu-II(Addpy)(2)Br-2(mu-Br-4)] (1), catena-poly[CuCl(mu-Addpy)(mu-Cl)CuCl2](n) (2) and [Cu(Addpy)(NCS)(2)] (3) were synthesized. Complexes are characterized by X-ray crystallography, spectral (UV-Vis, FTIR), electrochemical (CV) analyses, and magnetochemical measurements. Investigation of anticancer potential of Cu(II) complexes, mode of cell death, apoptosis, and inhibition of angiogenesis were performed. All tested malignant cell lines (HeLa, LS174, A549, K562, and MDA-MB-231) showed high sensitivity to the examined Cu(II) complexes. It has been shown that the complexes induce apoptosis in the caspase 3-dependent manner, whereas the anti-angiogenic effects of 1, 2, and 3 have been confirmed in EA.hy926 cells using a tube formation assay. (C) 2016 Elsevier Masson SAS. All rights reserved.
PB  - Elsevier
T2  - European Journal of Medicinal Chemistry
T1  - Synthesis, characterization, cytotoxicity and antiangiogenic activity of copper(II) complexes with 1-adamantoyl hydrazone bearing pyridine rings
VL  - 115
SP  - 75
EP  - 81
DO  - 10.1016/j.ejmech.2016.03.003
ER  - 

@article{
author = "Rodić, Marko V. and Leovac, Vukadin M. and Jovanović, Ljiljana S. and Spasojević, Vojislav and Joksović, Milan D. and Stanojković, Tatjana P. and Matić, Ivana Z. and Vojinović-Ješić, Ljiljana S. and Marković, Violeta",
year = "2016",
abstract = "Three novel copper complexes with tridentate N2O ligand di(2-pyridil) ketone 1-adamantoyl hydrazone (Addpy) of the formula [(Cu2Cu2I)-Cu-II(Addpy)(2)Br-2(mu-Br-4)] (1), catena-poly[CuCl(mu-Addpy)(mu-Cl)CuCl2](n) (2) and [Cu(Addpy)(NCS)(2)] (3) were synthesized. Complexes are characterized by X-ray crystallography, spectral (UV-Vis, FTIR), electrochemical (CV) analyses, and magnetochemical measurements. Investigation of anticancer potential of Cu(II) complexes, mode of cell death, apoptosis, and inhibition of angiogenesis were performed. All tested malignant cell lines (HeLa, LS174, A549, K562, and MDA-MB-231) showed high sensitivity to the examined Cu(II) complexes. It has been shown that the complexes induce apoptosis in the caspase 3-dependent manner, whereas the anti-angiogenic effects of 1, 2, and 3 have been confirmed in EA.hy926 cells using a tube formation assay. (C) 2016 Elsevier Masson SAS. All rights reserved.",
publisher = "Elsevier",
journal = "European Journal of Medicinal Chemistry",
title = "Synthesis, characterization, cytotoxicity and antiangiogenic activity of copper(II) complexes with 1-adamantoyl hydrazone bearing pyridine rings",
volume = "115",
pages = "75-81",
doi = "10.1016/j.ejmech.2016.03.003"
}

Rodić, M. V., Leovac, V. M., Jovanović, L. S., Spasojević, V., Joksović, M. D., Stanojković, T. P., Matić, I. Z., Vojinović-Ješić, L. S.,& Marković, V.. (2016). Synthesis, characterization, cytotoxicity and antiangiogenic activity of copper(II) complexes with 1-adamantoyl hydrazone bearing pyridine rings. in European Journal of Medicinal Chemistry
Elsevier., 115, 75-81.
https://doi.org/10.1016/j.ejmech.2016.03.003

Rodić MV, Leovac VM, Jovanović LS, Spasojević V, Joksović MD, Stanojković TP, Matić IZ, Vojinović-Ješić LS, Marković V. Synthesis, characterization, cytotoxicity and antiangiogenic activity of copper(II) complexes with 1-adamantoyl hydrazone bearing pyridine rings. in European Journal of Medicinal Chemistry. 2016;115:75-81.
doi:10.1016/j.ejmech.2016.03.003 .

Rodić, Marko V., Leovac, Vukadin M., Jovanović, Ljiljana S., Spasojević, Vojislav, Joksović, Milan D., Stanojković, Tatjana P., Matić, Ivana Z., Vojinović-Ješić, Ljiljana S., Marković, Violeta, "Synthesis, characterization, cytotoxicity and antiangiogenic activity of copper(II) complexes with 1-adamantoyl hydrazone bearing pyridine rings" in European Journal of Medicinal Chemistry, 115 (2016):75-81,
https://doi.org/10.1016/j.ejmech.2016.03.003 . .

TY  - JOUR
AU  - Joksimović, Nenad
AU  - Baskic, Dejan
AU  - Popovic, Suzana
AU  - Zarić, Milan
AU  - Kosanić, Marijana
AU  - Ranković, Branislav
AU  - Stanojković, Tatjana P.
AU  - Novaković, Slađana B.
AU  - Davidovic, Goran
AU  - Bugarčič, Zorica M.
AU  - Janković, Nenad
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1269
AB  - A serie of novel square pyramidal copper(II) complexes [Cu(L)(2)H2O] (3a-d) with O,O-bidentate ligands [L = ethyl-2-hydroxy-4-aryl-4-oxo-2-butenoate; aryl = 3-methoxyphenyl-2a, (E)-2-phenylvinyl-2b, (E)-2-(4-hydroxy-3-methoxyphenyl)vinyl-2c, 3-nitrophenyl-2d, 2-thienyl-2e] were synthesized and characterized by spectral (UV-Vis, IR, ESI-MS and EPR), elemental and X-ray analysis. The antimicrobial activity was estimated by the determination of the minimal inhibitory concentration (MIC) using the broth micro-dilution method. The most active antibacterial compounds were 3c and 3d, while the best antifungal activity was showed by complexes 3b and 3e. The lowest MIC value (0.048 mg mL(-1)) was measured for 3c against Proteus mirabilis. The cytotoxic activity was tested using the MTT method on human epithelial carcinoma HeLa cells, human lung carcinoma A549 cells and human colon carcinoma LS174 cells. All complexes showed extremely better cytotoxic activity compared to cisplatin at all tested concentrations. Compound 3d expressed the best activity against all tested cell lines with IC50 values ranging from 7.45 to 7.91 mu g mL(-1). The type of cell death and the impact on the cell cycle for 3d and 3e were evaluated by flow cytometry. Both compounds induced apoptosis and S phase cell cycle arrest. The interactions between selected complexes (3d and 3e) and CT-DNA or bovine serum albumin (BSA) were investigated by the fluorescence spectroscopic method. Competitive experiments with ethidium bromide (EB) indicated that 3d and 3e have a propensity to displace EB from the EB-DNA complex through intercalation suggesting strong competition with EB [K-sv = (1.4 +/- 0.2) and (2.9 +/- 0.1) x 10(4) M-1, respectively]. K-sv values indicate that these complexes bind to DNA covalently and non-covalently. The achieved results in the fluorescence titration of BSA with 3d and 3e [K-a = (2.9 +/- 0.2) x 10(6) and (2.5 +/- 0.2) x 10(5) M, respectively] showed that the fluorescence quenching of BSA is a result of the formation of the 3d- and 3e-BSA complexes. The obtained K-a values are high enough to ensure that a significant amount of 3d and 3e gets transported and distributed through the cells.
T2  - Dalton Transactions
T1  - Synthesis, characterization, biological activity, DNA and BSA binding study: novel copper(II) complexes with 2-hydroxy-4-aryl-4-oxo-2-butenoate
VL  - 45
IS  - 38
SP  - 15067
EP  - 15077
DO  - 10.1039/c6dt02257j
ER  - 

@article{
author = "Joksimović, Nenad and Baskic, Dejan and Popovic, Suzana and Zarić, Milan and Kosanić, Marijana and Ranković, Branislav and Stanojković, Tatjana P. and Novaković, Slađana B. and Davidovic, Goran and Bugarčič, Zorica M. and Janković, Nenad",
year = "2016",
abstract = "A serie of novel square pyramidal copper(II) complexes [Cu(L)(2)H2O] (3a-d) with O,O-bidentate ligands [L = ethyl-2-hydroxy-4-aryl-4-oxo-2-butenoate; aryl = 3-methoxyphenyl-2a, (E)-2-phenylvinyl-2b, (E)-2-(4-hydroxy-3-methoxyphenyl)vinyl-2c, 3-nitrophenyl-2d, 2-thienyl-2e] were synthesized and characterized by spectral (UV-Vis, IR, ESI-MS and EPR), elemental and X-ray analysis. The antimicrobial activity was estimated by the determination of the minimal inhibitory concentration (MIC) using the broth micro-dilution method. The most active antibacterial compounds were 3c and 3d, while the best antifungal activity was showed by complexes 3b and 3e. The lowest MIC value (0.048 mg mL(-1)) was measured for 3c against Proteus mirabilis. The cytotoxic activity was tested using the MTT method on human epithelial carcinoma HeLa cells, human lung carcinoma A549 cells and human colon carcinoma LS174 cells. All complexes showed extremely better cytotoxic activity compared to cisplatin at all tested concentrations. Compound 3d expressed the best activity against all tested cell lines with IC50 values ranging from 7.45 to 7.91 mu g mL(-1). The type of cell death and the impact on the cell cycle for 3d and 3e were evaluated by flow cytometry. Both compounds induced apoptosis and S phase cell cycle arrest. The interactions between selected complexes (3d and 3e) and CT-DNA or bovine serum albumin (BSA) were investigated by the fluorescence spectroscopic method. Competitive experiments with ethidium bromide (EB) indicated that 3d and 3e have a propensity to displace EB from the EB-DNA complex through intercalation suggesting strong competition with EB [K-sv = (1.4 +/- 0.2) and (2.9 +/- 0.1) x 10(4) M-1, respectively]. K-sv values indicate that these complexes bind to DNA covalently and non-covalently. The achieved results in the fluorescence titration of BSA with 3d and 3e [K-a = (2.9 +/- 0.2) x 10(6) and (2.5 +/- 0.2) x 10(5) M, respectively] showed that the fluorescence quenching of BSA is a result of the formation of the 3d- and 3e-BSA complexes. The obtained K-a values are high enough to ensure that a significant amount of 3d and 3e gets transported and distributed through the cells.",
journal = "Dalton Transactions",
title = "Synthesis, characterization, biological activity, DNA and BSA binding study: novel copper(II) complexes with 2-hydroxy-4-aryl-4-oxo-2-butenoate",
volume = "45",
number = "38",
pages = "15067-15077",
doi = "10.1039/c6dt02257j"
}

Joksimović, N., Baskic, D., Popovic, S., Zarić, M., Kosanić, M., Ranković, B., Stanojković, T. P., Novaković, S. B., Davidovic, G., Bugarčič, Z. M.,& Janković, N.. (2016). Synthesis, characterization, biological activity, DNA and BSA binding study: novel copper(II) complexes with 2-hydroxy-4-aryl-4-oxo-2-butenoate. in Dalton Transactions, 45(38), 15067-15077.
https://doi.org/10.1039/c6dt02257j

Joksimović N, Baskic D, Popovic S, Zarić M, Kosanić M, Ranković B, Stanojković TP, Novaković SB, Davidovic G, Bugarčič ZM, Janković N. Synthesis, characterization, biological activity, DNA and BSA binding study: novel copper(II) complexes with 2-hydroxy-4-aryl-4-oxo-2-butenoate. in Dalton Transactions. 2016;45(38):15067-15077.
doi:10.1039/c6dt02257j .

Joksimović, Nenad, Baskic, Dejan, Popovic, Suzana, Zarić, Milan, Kosanić, Marijana, Ranković, Branislav, Stanojković, Tatjana P., Novaković, Slađana B., Davidovic, Goran, Bugarčič, Zorica M., Janković, Nenad, "Synthesis, characterization, biological activity, DNA and BSA binding study: novel copper(II) complexes with 2-hydroxy-4-aryl-4-oxo-2-butenoate" in Dalton Transactions, 45, no. 38 (2016):15067-15077,
https://doi.org/10.1039/c6dt02257j . .

TY  - JOUR
AU  - Sabo, Tibor J.
AU  - Dinovic, VA
AU  - Kaluđerović, Goran N.
AU  - Stanojković, Tatjana P.
AU  - Bogdanović, Goran A.
AU  - Juranic, ZD
PY  - 2005
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2876
AB  - Four platinum(IV) complexes, trans,trans-dichlorobis(N,N-dimethylglycinato)platinum(IV), trans,trans-[Pt(dmgly)(2)Cl-2] (1) and trans.trans-dibromobis(N,N-dimethylglycinato)platinum(IV), trans,trans-[Pt(dmgly)(2)Br-2] (2), as well as, trans,trans-dichlorobis(N-methylglycinato)platinum(IV), trans,trans-[Pt(sar)(2)Cl-2] (3) and trans,trans-dibromobis(N-methylglycinato)platinum(IV), trans,trans-[Pt(sar)(2)Br-2] (4) (with configuration index for all complexes OC-6-14), were synthesized and characterized by elemental analysis, infrared and H-1 NMR spectroscopy. In the aim to assess the selectivity in the antitumor action of these complexes, the antiproliferative action of these compounds was determined to human adenocarcinoma HeLa cells; to human myelogenous leukemia K562 cells and to normal immunocompetent cells; i.e., on human PBMC. The details of the crystal structure synthesized trans,trans-[Pt(sar)(2)Br-2] complex were also reported here. In the crystal structure of trans, trans-[Pt(sar)(2)Br-2] the Pt(IV) ion had a deformed octahedral coordination with both N-methylglycinates and bromides bonded trans to one another and with the NPt-Br bond angles of 84.1(4) and 95.9(4)degrees. The trans, trans-[Pt(sar)(2)Br-2] complex molecules form 2D-layers with multiple N-H (...) O and C-H (...) O hydrogen bonds. (c) 2005 Elsevier B.V. All rights reserved.
T2  - Inorganica Chimica Acta
T1  - Syntheses and activity of some platinum(IV) complexes with N-methyl derivate of glycine and halogeno ligands against HeLa, K562 cell lines and human PBMC
VL  - 358
IS  - 7
SP  - 2239
EP  - 2245
DO  - 10.1016/j.ica.2005.01.007
ER  - 

@article{
author = "Sabo, Tibor J. and Dinovic, VA and Kaluđerović, Goran N. and Stanojković, Tatjana P. and Bogdanović, Goran A. and Juranic, ZD",
year = "2005",
abstract = "Four platinum(IV) complexes, trans,trans-dichlorobis(N,N-dimethylglycinato)platinum(IV), trans,trans-[Pt(dmgly)(2)Cl-2] (1) and trans.trans-dibromobis(N,N-dimethylglycinato)platinum(IV), trans,trans-[Pt(dmgly)(2)Br-2] (2), as well as, trans,trans-dichlorobis(N-methylglycinato)platinum(IV), trans,trans-[Pt(sar)(2)Cl-2] (3) and trans,trans-dibromobis(N-methylglycinato)platinum(IV), trans,trans-[Pt(sar)(2)Br-2] (4) (with configuration index for all complexes OC-6-14), were synthesized and characterized by elemental analysis, infrared and H-1 NMR spectroscopy. In the aim to assess the selectivity in the antitumor action of these complexes, the antiproliferative action of these compounds was determined to human adenocarcinoma HeLa cells; to human myelogenous leukemia K562 cells and to normal immunocompetent cells; i.e., on human PBMC. The details of the crystal structure synthesized trans,trans-[Pt(sar)(2)Br-2] complex were also reported here. In the crystal structure of trans, trans-[Pt(sar)(2)Br-2] the Pt(IV) ion had a deformed octahedral coordination with both N-methylglycinates and bromides bonded trans to one another and with the NPt-Br bond angles of 84.1(4) and 95.9(4)degrees. The trans, trans-[Pt(sar)(2)Br-2] complex molecules form 2D-layers with multiple N-H (...) O and C-H (...) O hydrogen bonds. (c) 2005 Elsevier B.V. All rights reserved.",
journal = "Inorganica Chimica Acta",
title = "Syntheses and activity of some platinum(IV) complexes with N-methyl derivate of glycine and halogeno ligands against HeLa, K562 cell lines and human PBMC",
volume = "358",
number = "7",
pages = "2239-2245",
doi = "10.1016/j.ica.2005.01.007"
}

Sabo, T. J., Dinovic, V., Kaluđerović, G. N., Stanojković, T. P., Bogdanović, G. A.,& Juranic, Z.. (2005). Syntheses and activity of some platinum(IV) complexes with N-methyl derivate of glycine and halogeno ligands against HeLa, K562 cell lines and human PBMC. in Inorganica Chimica Acta, 358(7), 2239-2245.
https://doi.org/10.1016/j.ica.2005.01.007

Sabo TJ, Dinovic V, Kaluđerović GN, Stanojković TP, Bogdanović GA, Juranic Z. Syntheses and activity of some platinum(IV) complexes with N-methyl derivate of glycine and halogeno ligands against HeLa, K562 cell lines and human PBMC. in Inorganica Chimica Acta. 2005;358(7):2239-2245.
doi:10.1016/j.ica.2005.01.007 .

Sabo, Tibor J., Dinovic, VA, Kaluđerović, Goran N., Stanojković, Tatjana P., Bogdanović, Goran A., Juranic, ZD, "Syntheses and activity of some platinum(IV) complexes with N-methyl derivate of glycine and halogeno ligands against HeLa, K562 cell lines and human PBMC" in Inorganica Chimica Acta, 358, no. 7 (2005):2239-2245,
https://doi.org/10.1016/j.ica.2005.01.007 . .