TY  - JOUR
AU  - Mićović, Žarko
AU  - Kostić, Sanja
AU  - Mutavdžin, Slavica
AU  - Andrejević, Aleksa
AU  - Stamenković, Aleksandra
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
AU  - Đurić, Marko
AU  - Hrnčić, Dragan
AU  - Živković, Vladimir
AU  - Jakovljević, Vladimir
AU  - Đurić, Dragan
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12970
AB  - Background/Aim. Chronically induced hypermethioninemia leads to hyperhomocysteinemia which causes oxidative stress, atherogenesis, neurodegeneration and cancer. However, little is known about the acute and subchronic effects of DL-methionine (Met). The aim of study was to assess the effects of acutely and subchronically applied Met on oxidative stress parameters in rat plasma [enzymes: catalase (CAT), glutathione peroxidise (GPx), superoxide dismutase (SOD) and index of lipid peroxidation, malondialdehyde (MDA)], and acetylcholinesterase (AChE) activity in rat cardiac tissue. Methods. The enzymes activities, as well as MDA concentration were evaluated following acute (n = 8) and subchronic (n = 10) application of Met [i.p. 0.8 mmoL/kg body weight (b.w.) in a single dose in the acute overload or daily during three weeks in the subchronic overload]. The same was done in the control groups following application of physiological solution [i.p. 1 mL 0.9% NaCl (n = 8) in the acute overload and 0.1–0.2 mL 0.9% NaCl, daily during three weeks (n =10) in the subchronic overload]. Tested parameters were evaluated 60 minutes after application in acute experiments and after three weeks of treatment in subchronic experiments. Results. There were no difference in homocysteine values between the groups treated with Met for three weeks and the control group. Met administration significantly increased the activity of CAT and GPx after 1 h compared to the control group (p = 0.008 for both enzymes), whereas the activity of SOD and MDA concentrations were unchanged. Subchronically applied Met did not affect activity of antioxidant enzymes and MDA level. AChE activity did not show any change in rat cardiac tissue after 1 h, but it was significantly decreased after the subchronic treatment (p = 0.041). Conclusion. Results of present research indicate that Met differently affects estimated parameters during acute and subchronic application. In the acute treatment Met mobilizes the most part of antioxidant enzymes while during the subchronic treatment these changes seems to be lost. On the contrary, the acute Met overload was not sufficient to influence on the AChE activity, while longer duration of Met loading diminished function of the enzyme. These findings point out that methionine can interfere with antioxidant defense system and cholinergic control of the heart function.
T2  - Vojnosanitetski pregled
T1  - The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue
VL  - 77
IS  - 2
SP  - 165
EP  - 173
DO  - 10.2298/VSP171213055M
ER  - 

@article{
author = "Mićović, Žarko and Kostić, Sanja and Mutavdžin, Slavica and Andrejević, Aleksa and Stamenković, Aleksandra and Čolović, Mirjana and Krstić, Danijela and Đurić, Marko and Hrnčić, Dragan and Živković, Vladimir and Jakovljević, Vladimir and Đurić, Dragan",
year = "2020",
abstract = "Background/Aim. Chronically induced hypermethioninemia leads to hyperhomocysteinemia which causes oxidative stress, atherogenesis, neurodegeneration and cancer. However, little is known about the acute and subchronic effects of DL-methionine (Met). The aim of study was to assess the effects of acutely and subchronically applied Met on oxidative stress parameters in rat plasma [enzymes: catalase (CAT), glutathione peroxidise (GPx), superoxide dismutase (SOD) and index of lipid peroxidation, malondialdehyde (MDA)], and acetylcholinesterase (AChE) activity in rat cardiac tissue. Methods. The enzymes activities, as well as MDA concentration were evaluated following acute (n = 8) and subchronic (n = 10) application of Met [i.p. 0.8 mmoL/kg body weight (b.w.) in a single dose in the acute overload or daily during three weeks in the subchronic overload]. The same was done in the control groups following application of physiological solution [i.p. 1 mL 0.9% NaCl (n = 8) in the acute overload and 0.1–0.2 mL 0.9% NaCl, daily during three weeks (n =10) in the subchronic overload]. Tested parameters were evaluated 60 minutes after application in acute experiments and after three weeks of treatment in subchronic experiments. Results. There were no difference in homocysteine values between the groups treated with Met for three weeks and the control group. Met administration significantly increased the activity of CAT and GPx after 1 h compared to the control group (p = 0.008 for both enzymes), whereas the activity of SOD and MDA concentrations were unchanged. Subchronically applied Met did not affect activity of antioxidant enzymes and MDA level. AChE activity did not show any change in rat cardiac tissue after 1 h, but it was significantly decreased after the subchronic treatment (p = 0.041). Conclusion. Results of present research indicate that Met differently affects estimated parameters during acute and subchronic application. In the acute treatment Met mobilizes the most part of antioxidant enzymes while during the subchronic treatment these changes seems to be lost. On the contrary, the acute Met overload was not sufficient to influence on the AChE activity, while longer duration of Met loading diminished function of the enzyme. These findings point out that methionine can interfere with antioxidant defense system and cholinergic control of the heart function.",
journal = "Vojnosanitetski pregled",
title = "The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue",
volume = "77",
number = "2",
pages = "165-173",
doi = "10.2298/VSP171213055M"
}

Mićović, Ž., Kostić, S., Mutavdžin, S., Andrejević, A., Stamenković, A., Čolović, M., Krstić, D., Đurić, M., Hrnčić, D., Živković, V., Jakovljević, V.,& Đurić, D.. (2020). The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue. in Vojnosanitetski pregled, 77(2), 165-173.
https://doi.org/10.2298/VSP171213055M

Mićović Ž, Kostić S, Mutavdžin S, Andrejević A, Stamenković A, Čolović M, Krstić D, Đurić M, Hrnčić D, Živković V, Jakovljević V, Đurić D. The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue. in Vojnosanitetski pregled. 2020;77(2):165-173.
doi:10.2298/VSP171213055M .

Mićović, Žarko, Kostić, Sanja, Mutavdžin, Slavica, Andrejević, Aleksa, Stamenković, Aleksandra, Čolović, Mirjana, Krstić, Danijela, Đurić, Marko, Hrnčić, Dragan, Živković, Vladimir, Jakovljević, Vladimir, Đurić, Dragan, "The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue" in Vojnosanitetski pregled, 77, no. 2 (2020):165-173,
https://doi.org/10.2298/VSP171213055M . .

TY  - CONF
AU  - Kostić, Sanja
AU  - Đurić, Marko
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
AU  - Đurić, Dragan
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12971
AB  - Background and Aims: Homocysteine (Hcy) is well recognized as an independent risk factor for atherosclerosis and endothelial dysfunction. Among other mechanisms, it has been shown that induction of oxidative stress is involved. There are also increasing number of assumptions that signaling gaseous molecules might have an important role in effects of Hcy on cardiovascular function. Thus, the aim of the study is to evaluate and compare the effects of Hcy and DL-PAG (H2S production inhibitor) on oxidative stress parameters in plasma, as well as the effects of their combination and possible interaction. Methods: Male rats were divided in 4 groups (n¼10 in each): 1) Control groupesaline (1 ml 0.9% NaCl i.p., pH 7.4); 2) DL-Hcy (8 mmol/kg i.p.); 3) DL-PAG (50 mg//kg i.p.); 4) DL-Hcy+DL-PAG (8 mmol/kg i.p. DL-Hcy+50 mg//kg i.p. DL-PAG). Index of lipid peroxidation (MDA) and antioxidant enzymes-catalase, glutathione peroxidase, superoxide dismutase (SOD) were examined by spectrophotometer 60 minutes after application. Results: Hcy increased activities of antioxidant enzymes in rat plasma, but only SOD with statistical significance. This is probably as an adaptive/ compensatory response to its pro-oxidant effects. H2S inhibiton induced statistically significant increase of all antioxidant enzymes. Consequently, MDA in both goups were significantly reduced. Interestingly, combination of Hcy and DL-PAG induced lesser increase than DL-PAG alone, except for SOD. Conclusions: Since only SOD remained the same, probably that Hcy especially increases superoxide anion radical production, whereas H2S inhibition also affects hydrogen peroxide and some other oxidative stress markers. Further investigations are required to reveal finer interactions of Hcy and gaseous molecules.
C3  - Atherosclerosis
T1  - The Effects Of Hydrogen Sulfide Synthase Inhibition Following Acute Administration Of Homocysteine On Oxidative Stress Markers In Rat Plasma
VL  - 287
SP  - e274
EP  - e274
DO  - 10.1016/j.atherosclerosis.2019.06.849
ER  - 

@conference{
author = "Kostić, Sanja and Đurić, Marko and Čolović, Mirjana and Krstić, Danijela and Đurić, Dragan",
year = "2019",
abstract = "Background and Aims: Homocysteine (Hcy) is well recognized as an independent risk factor for atherosclerosis and endothelial dysfunction. Among other mechanisms, it has been shown that induction of oxidative stress is involved. There are also increasing number of assumptions that signaling gaseous molecules might have an important role in effects of Hcy on cardiovascular function. Thus, the aim of the study is to evaluate and compare the effects of Hcy and DL-PAG (H2S production inhibitor) on oxidative stress parameters in plasma, as well as the effects of their combination and possible interaction. Methods: Male rats were divided in 4 groups (n¼10 in each): 1) Control groupesaline (1 ml 0.9% NaCl i.p., pH 7.4); 2) DL-Hcy (8 mmol/kg i.p.); 3) DL-PAG (50 mg//kg i.p.); 4) DL-Hcy+DL-PAG (8 mmol/kg i.p. DL-Hcy+50 mg//kg i.p. DL-PAG). Index of lipid peroxidation (MDA) and antioxidant enzymes-catalase, glutathione peroxidase, superoxide dismutase (SOD) were examined by spectrophotometer 60 minutes after application. Results: Hcy increased activities of antioxidant enzymes in rat plasma, but only SOD with statistical significance. This is probably as an adaptive/ compensatory response to its pro-oxidant effects. H2S inhibiton induced statistically significant increase of all antioxidant enzymes. Consequently, MDA in both goups were significantly reduced. Interestingly, combination of Hcy and DL-PAG induced lesser increase than DL-PAG alone, except for SOD. Conclusions: Since only SOD remained the same, probably that Hcy especially increases superoxide anion radical production, whereas H2S inhibition also affects hydrogen peroxide and some other oxidative stress markers. Further investigations are required to reveal finer interactions of Hcy and gaseous molecules.",
journal = "Atherosclerosis",
title = "The Effects Of Hydrogen Sulfide Synthase Inhibition Following Acute Administration Of Homocysteine On Oxidative Stress Markers In Rat Plasma",
volume = "287",
pages = "e274-e274",
doi = "10.1016/j.atherosclerosis.2019.06.849"
}

Kostić, S., Đurić, M., Čolović, M., Krstić, D.,& Đurić, D.. (2019). The Effects Of Hydrogen Sulfide Synthase Inhibition Following Acute Administration Of Homocysteine On Oxidative Stress Markers In Rat Plasma. in Atherosclerosis, 287, e274-e274.
https://doi.org/10.1016/j.atherosclerosis.2019.06.849

Kostić S, Đurić M, Čolović M, Krstić D, Đurić D. The Effects Of Hydrogen Sulfide Synthase Inhibition Following Acute Administration Of Homocysteine On Oxidative Stress Markers In Rat Plasma. in Atherosclerosis. 2019;287:e274-e274.
doi:10.1016/j.atherosclerosis.2019.06.849 .

Kostić, Sanja, Đurić, Marko, Čolović, Mirjana, Krstić, Danijela, Đurić, Dragan, "The Effects Of Hydrogen Sulfide Synthase Inhibition Following Acute Administration Of Homocysteine On Oxidative Stress Markers In Rat Plasma" in Atherosclerosis, 287 (2019):e274-e274,
https://doi.org/10.1016/j.atherosclerosis.2019.06.849 . .

TY  - JOUR
AU  - Đurić, Marko
AU  - Mutavdžin, Slavica
AU  - Lončar-Stojiljković, Dragana
AU  - Kostić, Sanja
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
AU  - Živković, Vladimir
AU  - Jakovljević, Vladimir
AU  - Đurić, Dragan
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12972
AB  - Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group – saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ sacrifice. AChE activity was measured in the rats’ cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+LNAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters.
T2  - Scripta Medica
T1  - The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity
VL  - 50
IS  - 3
SP  - 112
EP  - 116
DO  - 10.5937/scriptamed50-22658
ER  - 

@article{
author = "Đurić, Marko and Mutavdžin, Slavica and Lončar-Stojiljković, Dragana and Kostić, Sanja and Čolović, Mirjana and Krstić, Danijela and Živković, Vladimir and Jakovljević, Vladimir and Đurić, Dragan",
year = "2019",
abstract = "Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group – saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ sacrifice. AChE activity was measured in the rats’ cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+LNAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters.",
journal = "Scripta Medica",
title = "The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity",
volume = "50",
number = "3",
pages = "112-116",
doi = "10.5937/scriptamed50-22658"
}

Đurić, M., Mutavdžin, S., Lončar-Stojiljković, D., Kostić, S., Čolović, M., Krstić, D., Živković, V., Jakovljević, V.,& Đurić, D.. (2019). The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity. in Scripta Medica, 50(3), 112-116.
https://doi.org/10.5937/scriptamed50-22658

Đurić M, Mutavdžin S, Lončar-Stojiljković D, Kostić S, Čolović M, Krstić D, Živković V, Jakovljević V, Đurić D. The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity. in Scripta Medica. 2019;50(3):112-116.
doi:10.5937/scriptamed50-22658 .

Đurić, Marko, Mutavdžin, Slavica, Lončar-Stojiljković, Dragana, Kostić, Sanja, Čolović, Mirjana, Krstić, Danijela, Živković, Vladimir, Jakovljević, Vladimir, Đurić, Dragan, "The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity" in Scripta Medica, 50, no. 3 (2019):112-116,
https://doi.org/10.5937/scriptamed50-22658 . .

TY  - JOUR
AU  - Đurić, Marko
AU  - Kostić, Sanja
AU  - Lončar-Stojiljković, Dragana
AU  - Mutavdžin, Slavica
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
AU  - Stevanović, Predrag
AU  - Đurić, Dragan
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12973
AB  - Background: The importance of homocysteine (Hcy) is increasingly recognized in last few decades as an independent risk factor for atherosclerosis and thrombosis, but there is lack of data referring to influence of Hcy on plasma oxidative stress parameters as well as the role of gasotransmitters in these effects. Therefore, this study aim was to assess the role of gasotransmitter inhibitors in Hcy-induced effects on plasma oxidative stress in rats. Material and Methods: Study involved 96 male Wistar albino rats divided into 8 groups: 1) Control group - saline (1ml 0.9 % NaCl i.p.); 2) DL-Hcy (8 mmol/kg i.p. DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg i.p. Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) ZnPPR IX (30 mol/kg i.p. protoporphyrin IX zinc (ZnPPR IX), inhibitor of CO production); 5) DL-PAG (50 mg//kg i.p. DL-propargylglycine (DL-PAG), inhibitor of H2S production); 6) DL-Hcy+L-NAME (8 mmol/kg i.p. DL-Hcy + 10 mg/kg i.p. L-NAME); 7) DL-Hcy+ZnPPR IX (8 mmol/kg i.p. DL-Hcy + 30 mol/kg i.p. Zn PPR IX), and 8) DL-Hcy+DL-PAG (8 mmol/kg i.p. DL-Hcy + 50 mg//kg i.p. DL-PAG). In all experimental groups, tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals' euthanasia. In the collected blood samples malondialdehyde concentration, catalase, glutathione peroxidase and superoxide dismutase activity were measured. Results: Applied substances induced rapid and strong increase of plasma antioxidant enzymatic activity probably as a compensatory response to its pro-oxidant influence. Conclusion: The effects of Hcy on the activity of plasma antioxidant enzymes are in part mediated via interaction with gasotransmitters.
T2  - Scripta Medica
T1  - The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma
VL  - 50
IS  - 1
SP  - 6
EP  - 12
DO  - 10.5937/scriptamed50-21100
ER  - 

@article{
author = "Đurić, Marko and Kostić, Sanja and Lončar-Stojiljković, Dragana and Mutavdžin, Slavica and Čolović, Mirjana and Krstić, Danijela and Stevanović, Predrag and Đurić, Dragan",
year = "2019",
abstract = "Background: The importance of homocysteine (Hcy) is increasingly recognized in last few decades as an independent risk factor for atherosclerosis and thrombosis, but there is lack of data referring to influence of Hcy on plasma oxidative stress parameters as well as the role of gasotransmitters in these effects. Therefore, this study aim was to assess the role of gasotransmitter inhibitors in Hcy-induced effects on plasma oxidative stress in rats. Material and Methods: Study involved 96 male Wistar albino rats divided into 8 groups: 1) Control group - saline (1ml 0.9 % NaCl i.p.); 2) DL-Hcy (8 mmol/kg i.p. DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg i.p. Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) ZnPPR IX (30 mol/kg i.p. protoporphyrin IX zinc (ZnPPR IX), inhibitor of CO production); 5) DL-PAG (50 mg//kg i.p. DL-propargylglycine (DL-PAG), inhibitor of H2S production); 6) DL-Hcy+L-NAME (8 mmol/kg i.p. DL-Hcy + 10 mg/kg i.p. L-NAME); 7) DL-Hcy+ZnPPR IX (8 mmol/kg i.p. DL-Hcy + 30 mol/kg i.p. Zn PPR IX), and 8) DL-Hcy+DL-PAG (8 mmol/kg i.p. DL-Hcy + 50 mg//kg i.p. DL-PAG). In all experimental groups, tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals' euthanasia. In the collected blood samples malondialdehyde concentration, catalase, glutathione peroxidase and superoxide dismutase activity were measured. Results: Applied substances induced rapid and strong increase of plasma antioxidant enzymatic activity probably as a compensatory response to its pro-oxidant influence. Conclusion: The effects of Hcy on the activity of plasma antioxidant enzymes are in part mediated via interaction with gasotransmitters.",
journal = "Scripta Medica",
title = "The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma",
volume = "50",
number = "1",
pages = "6-12",
doi = "10.5937/scriptamed50-21100"
}

Đurić, M., Kostić, S., Lončar-Stojiljković, D., Mutavdžin, S., Čolović, M., Krstić, D., Stevanović, P.,& Đurić, D.. (2019). The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma. in Scripta Medica, 50(1), 6-12.
https://doi.org/10.5937/scriptamed50-21100

Đurić M, Kostić S, Lončar-Stojiljković D, Mutavdžin S, Čolović M, Krstić D, Stevanović P, Đurić D. The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma. in Scripta Medica. 2019;50(1):6-12.
doi:10.5937/scriptamed50-21100 .

Đurić, Marko, Kostić, Sanja, Lončar-Stojiljković, Dragana, Mutavdžin, Slavica, Čolović, Mirjana, Krstić, Danijela, Stevanović, Predrag, Đurić, Dragan, "The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma" in Scripta Medica, 50, no. 1 (2019):6-12,
https://doi.org/10.5937/scriptamed50-21100 . .

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Vasić, Vesna M.
AU  - Đurić, Dragan M.
AU  - Krstić, Danijela Z.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1926
AB  - Background: Sulphur is an abundant element in biological systems, which plays an important role in processes essential for life as a constituent of proteins, vitamins and other crucial biomolecules. The major source of sulphur for humans is plants being able to use inorganic sulphur in the purpose of sulphur-containing amino acids synthesis. Sulphur-containing amino acids include methionine, cysteine, homocysteine, and taurine. Methionine and cysteine are classified as proteinogenic, canonic amino acids incorporated in protein structure. Sulphur amino acids are involved in the synthesis of intracellular antioxidants such as glutathione and N-acetyl cysteine. Moreover, naturally occurring sulphur-containing ligands are effective and safe detoxifying agents, often used in order to prevent toxic metal ions effects and their accumulation in human body. Methods: Literature search for peer-reviewed articles was performed using PubMed and Scopus databases, and utilizing appropriate keywords. Results: This review is focused on sulphur-containing amino acids - methionine, cysteine, taurine, and their derivatives - glutathione and N-acetylcysteine, and their defense effects as antioxidant agents against free radicals. Additionally, the protective effects of sulphur-containing ligands against the toxic effects of heavy and transition metal ions, and their reactivation role towards the enzyme inhibition are described. Conclusion: Sulphur-containing amino acids represent a powerful part of cell antioxidant system. Thus, they are essential in the maintenance of normal cellular functions and health. In addition to their worthy antioxidant action, sulphur-containing amino acids may offer a chelating site for heavy metals. Accordingly, they may be supplemented during chelating therapy, providing beneficial effects in eliminating toxic metals.
T2  - Current Medicinal Chemistry
T1  - Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals
VL  - 25
IS  - 3
SP  - 324
EP  - 335
DO  - 10.2174/0929867324666170609075434
ER  - 

@article{
author = "Čolović, Mirjana B. and Vasić, Vesna M. and Đurić, Dragan M. and Krstić, Danijela Z.",
year = "2018",
abstract = "Background: Sulphur is an abundant element in biological systems, which plays an important role in processes essential for life as a constituent of proteins, vitamins and other crucial biomolecules. The major source of sulphur for humans is plants being able to use inorganic sulphur in the purpose of sulphur-containing amino acids synthesis. Sulphur-containing amino acids include methionine, cysteine, homocysteine, and taurine. Methionine and cysteine are classified as proteinogenic, canonic amino acids incorporated in protein structure. Sulphur amino acids are involved in the synthesis of intracellular antioxidants such as glutathione and N-acetyl cysteine. Moreover, naturally occurring sulphur-containing ligands are effective and safe detoxifying agents, often used in order to prevent toxic metal ions effects and their accumulation in human body. Methods: Literature search for peer-reviewed articles was performed using PubMed and Scopus databases, and utilizing appropriate keywords. Results: This review is focused on sulphur-containing amino acids - methionine, cysteine, taurine, and their derivatives - glutathione and N-acetylcysteine, and their defense effects as antioxidant agents against free radicals. Additionally, the protective effects of sulphur-containing ligands against the toxic effects of heavy and transition metal ions, and their reactivation role towards the enzyme inhibition are described. Conclusion: Sulphur-containing amino acids represent a powerful part of cell antioxidant system. Thus, they are essential in the maintenance of normal cellular functions and health. In addition to their worthy antioxidant action, sulphur-containing amino acids may offer a chelating site for heavy metals. Accordingly, they may be supplemented during chelating therapy, providing beneficial effects in eliminating toxic metals.",
journal = "Current Medicinal Chemistry",
title = "Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals",
volume = "25",
number = "3",
pages = "324-335",
doi = "10.2174/0929867324666170609075434"
}

Čolović, M. B., Vasić, V. M., Đurić, D. M.,& Krstić, D. Z.. (2018). Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals. in Current Medicinal Chemistry, 25(3), 324-335.
https://doi.org/10.2174/0929867324666170609075434

Čolović MB, Vasić VM, Đurić DM, Krstić DZ. Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals. in Current Medicinal Chemistry. 2018;25(3):324-335.
doi:10.2174/0929867324666170609075434 .

Čolović, Mirjana B., Vasić, Vesna M., Đurić, Dragan M., Krstić, Danijela Z., "Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals" in Current Medicinal Chemistry, 25, no. 3 (2018):324-335,
https://doi.org/10.2174/0929867324666170609075434 . .

TY  - JOUR
AU  - Kornjača, Duško
AU  - Živković, Vladimir I.
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Marko
AU  - Stanković, Sanja
AU  - Mutavdžin, Slavica
AU  - Jakovljević, Vladimir Lj.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641700041K
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7714
AB  - The aim of this study was to assess the effects of DL-homocysteine (DL-Hcy) and DL-homocysteine thiolactone (DL-Hcy TLHC) on selected serum biochemical parameters, markers of oxidative stress and the activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) in the plasma, as well as on acetylcholinesterase (AChE) activity in the cardiac tissue homogenate in the rat. Male Wistar rats were divided into three groups as follows: control group (1 mL 0.9% NaCl, intraperitoneal (i.p.) injection), DL-Hcy group (8 mmol/kg body mass (b.m.), i.p.) or DL-Hcy TLHC group (8 mmol/kg b.m., i.p.). One hour after administration, the rats were euthanized, whole blood was collected for biochemical analysis, and the heart was excised. Following the i.p. administration of DL-Hcy and DL-Hcy TLHC, the activities of antioxidant enzymes were mostly significantly increased, while plasma malondialdehyde (MDA) was decreased. Administration of DL-Hcy and DL-Hcy TLHC significantly inhibited AChE activity in rat cardiac tissue. Our findings suggest that DL-Hcy and DL-Hcy TLHC exerted prooxidant effects; however, the decrease in MDA points to an inverse response to the increase in antioxidant enzyme activities. While both substances inhibited AChE activity in rat cardiac tissue, DL-Hcy TLHC induced stronger effects than DL-Hcy.
T2  - Archives of Biological Sciences
T1  - The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat
VL  - 70
IS  - 2
SP  - 241
EP  - 248
DO  - 10.2298/ABS170731041K
ER  - 

@article{
author = "Kornjača, Duško and Živković, Vladimir I. and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Marko and Stanković, Sanja and Mutavdžin, Slavica and Jakovljević, Vladimir Lj. and Đurić, Dragan M.",
year = "2018",
abstract = "The aim of this study was to assess the effects of DL-homocysteine (DL-Hcy) and DL-homocysteine thiolactone (DL-Hcy TLHC) on selected serum biochemical parameters, markers of oxidative stress and the activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) in the plasma, as well as on acetylcholinesterase (AChE) activity in the cardiac tissue homogenate in the rat. Male Wistar rats were divided into three groups as follows: control group (1 mL 0.9% NaCl, intraperitoneal (i.p.) injection), DL-Hcy group (8 mmol/kg body mass (b.m.), i.p.) or DL-Hcy TLHC group (8 mmol/kg b.m., i.p.). One hour after administration, the rats were euthanized, whole blood was collected for biochemical analysis, and the heart was excised. Following the i.p. administration of DL-Hcy and DL-Hcy TLHC, the activities of antioxidant enzymes were mostly significantly increased, while plasma malondialdehyde (MDA) was decreased. Administration of DL-Hcy and DL-Hcy TLHC significantly inhibited AChE activity in rat cardiac tissue. Our findings suggest that DL-Hcy and DL-Hcy TLHC exerted prooxidant effects; however, the decrease in MDA points to an inverse response to the increase in antioxidant enzyme activities. While both substances inhibited AChE activity in rat cardiac tissue, DL-Hcy TLHC induced stronger effects than DL-Hcy.",
journal = "Archives of Biological Sciences",
title = "The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat",
volume = "70",
number = "2",
pages = "241-248",
doi = "10.2298/ABS170731041K"
}

Kornjača, D., Živković, V. I., Krstić, D. Z., Čolović, M. B., Đurić, M., Stanković, S., Mutavdžin, S., Jakovljević, V. Lj.,& Đurić, D. M.. (2018). The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat. in Archives of Biological Sciences, 70(2), 241-248.
https://doi.org/10.2298/ABS170731041K

Kornjača D, Živković VI, Krstić DZ, Čolović MB, Đurić M, Stanković S, Mutavdžin S, Jakovljević VL, Đurić DM. The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat. in Archives of Biological Sciences. 2018;70(2):241-248.
doi:10.2298/ABS170731041K .

Kornjača, Duško, Živković, Vladimir I., Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Marko, Stanković, Sanja, Mutavdžin, Slavica, Jakovljević, Vladimir Lj., Đurić, Dragan M., "The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat" in Archives of Biological Sciences, 70, no. 2 (2018):241-248,
https://doi.org/10.2298/ABS170731041K . .

TY  - JOUR
AU  - Jakovljević-Uzelac, Jovana
AU  - Stanić, Marina
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s11010-017-3238-z
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7788
AB  - The objective of this study was to investigate in vitro effects of 10 A mu M dl-homocysteine (dl-Hcy), dl-homocysteine thiolactone-hydrochloride (dl-Hcy TLHC), and l-homocysteine thiolactone-hydrochloride (l-Hcy TLHC) on the oxygen consumption of rat heart tissue homogenate, as well as the involvement of the gasotransmitters NO, H2S and CO in the effects of the most toxic homocysteine compound, dl-Hcy TLHC. The possible contribution of the gasotransmitters in these effects was estimated by using the appropriate inhibitors of their synthesis (N (omega)-nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (dl-PAG), and zinc protoporphyrin IX (ZnPPR IX), respectively). The oxygen consumption of rat heart tissue homogenate was measured by Clark/type oxygen electrode in the absence and presence of the investigated compounds. All three homocysteine-based compounds caused a similar decrease in the oxygen consumption rate compared to control: 15.19 +/- 4.01%, 12.42 +/- 1.01%, and 16.43 +/- 4.52% for dl-Hcy, dl-Hcy TLHC, or l-Hcy TLHC, respectively. All applied inhibitors of gasotransmitter synthesis also decreased the oxygen consumption rate of tissue homogenate related to control: 13.53 +/- 1.35% for l-NAME (30 A mu M), 5.32 +/- 1.23% for dl-PAG (10 A mu M), and 5.56 +/- 1.39% for ZnPPR IX (10 A mu M). Simultaneous effect of l-NAME (30 A mu M) or ZnPPR IX (10 A mu M) with dl-Hcy TLHC (10 A mu M) caused a larger decrease of oxygen consumption compared to each of the substances individually. However, when dl-PAG (10 A mu M) was applied together with dl-Hcy TLHC (10 A mu M), it attenuated the effect of dl-Hcy TLHC from 12.42 +/- 1.01 to 9.22 +/- 1.58%. In conclusion, cardiotoxicity induced by Hcy-related compounds, which was shown in our previous research, could result from the inhibition of the oxygen consumption, and might be mediated by the certain gasotransmitters.
T2  - Molecular and Cellular Biochemistry
T1  - Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters
VL  - 444
IS  - 1-2
SP  - 143
EP  - 148
DO  - 10.1007/s11010-017-3238-z
ER  - 

@article{
author = "Jakovljević-Uzelac, Jovana and Stanić, Marina and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Dragan M.",
year = "2018",
abstract = "The objective of this study was to investigate in vitro effects of 10 A mu M dl-homocysteine (dl-Hcy), dl-homocysteine thiolactone-hydrochloride (dl-Hcy TLHC), and l-homocysteine thiolactone-hydrochloride (l-Hcy TLHC) on the oxygen consumption of rat heart tissue homogenate, as well as the involvement of the gasotransmitters NO, H2S and CO in the effects of the most toxic homocysteine compound, dl-Hcy TLHC. The possible contribution of the gasotransmitters in these effects was estimated by using the appropriate inhibitors of their synthesis (N (omega)-nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (dl-PAG), and zinc protoporphyrin IX (ZnPPR IX), respectively). The oxygen consumption of rat heart tissue homogenate was measured by Clark/type oxygen electrode in the absence and presence of the investigated compounds. All three homocysteine-based compounds caused a similar decrease in the oxygen consumption rate compared to control: 15.19 +/- 4.01%, 12.42 +/- 1.01%, and 16.43 +/- 4.52% for dl-Hcy, dl-Hcy TLHC, or l-Hcy TLHC, respectively. All applied inhibitors of gasotransmitter synthesis also decreased the oxygen consumption rate of tissue homogenate related to control: 13.53 +/- 1.35% for l-NAME (30 A mu M), 5.32 +/- 1.23% for dl-PAG (10 A mu M), and 5.56 +/- 1.39% for ZnPPR IX (10 A mu M). Simultaneous effect of l-NAME (30 A mu M) or ZnPPR IX (10 A mu M) with dl-Hcy TLHC (10 A mu M) caused a larger decrease of oxygen consumption compared to each of the substances individually. However, when dl-PAG (10 A mu M) was applied together with dl-Hcy TLHC (10 A mu M), it attenuated the effect of dl-Hcy TLHC from 12.42 +/- 1.01 to 9.22 +/- 1.58%. In conclusion, cardiotoxicity induced by Hcy-related compounds, which was shown in our previous research, could result from the inhibition of the oxygen consumption, and might be mediated by the certain gasotransmitters.",
journal = "Molecular and Cellular Biochemistry",
title = "Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters",
volume = "444",
number = "1-2",
pages = "143-148",
doi = "10.1007/s11010-017-3238-z"
}

Jakovljević-Uzelac, J., Stanić, M., Krstić, D. Z., Čolović, M. B.,& Đurić, D. M.. (2018). Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters. in Molecular and Cellular Biochemistry, 444(1-2), 143-148.
https://doi.org/10.1007/s11010-017-3238-z

Jakovljević-Uzelac J, Stanić M, Krstić DZ, Čolović MB, Đurić DM. Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters. in Molecular and Cellular Biochemistry. 2018;444(1-2):143-148.
doi:10.1007/s11010-017-3238-z .

Jakovljević-Uzelac, Jovana, Stanić, Marina, Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Dragan M., "Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters" in Molecular and Cellular Biochemistry, 444, no. 1-2 (2018):143-148,
https://doi.org/10.1007/s11010-017-3238-z . .

TY  - CONF
AU  - Đurić, Aleksandar
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Obrenović, Radmila
AU  - Micovic, Z
AU  - Đurić, M.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0021915018309547
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7954
AB  - Aim: Methionine load could lead to hyperhomocysteinemia as well to changes of certain biochemical and inflammation markers. However it is not clear whether other sulfur containing amino acids should affect methionine load effects. The aim was to examine the effects of subchronic methionine (Met) overload on standard biochemical parameters and markers of homocysteine metabolism in rat blood, and to examine whether subchronic administration of N-acetyl-L-cysteine (NAC) affected mentioned parameters.

Methods: The research was conducted during a three-week period (male Wistar albino rats, n=20, body weight of approximately 160g, age of 15-20 days), and the animals were divided into a control and experimental groups, consisted of 10 animals each: a) control group (i.p. 0.1ml/day 0.9%NaCl); b) methionine (0.8 mmol/kg/bw/day); c) methionine (0.8 mmol/kg/bw/day) plus NAC (50 mg/kg/bw/day).

Results: It was found statistically altered levels of certain parameters in NAC plus Met load group vs. control (BUN, CREA, URCA, TBI, TP, ALB, AHDLA, TGL, Na, K, AMY, ACRP, respectively) as well in Met group vs. control. Interestingly, homocysteine level was not increased (control 9.98±0.65 vs. NAC plus Met load 10.21±0.71 μmol/L, P>0.05), but folate was significantly decreased (35.25±2.04 vs. 29.23±1.48 μg/L, P<0.05) as well as vitamin B12 level (882.00±32.00 vs. 756.62±43.84 ng/L, P<0.05). This trend of results was different if it was compared with Met load group vs. control.

Conclusions: The obtained results indicated that monitoring of standard biochemical parameters and homocysteine metabolism markers in blood could provide a valuable insight in the evaluation of NAC plus methionine effects, which may be useful for further research.
C3  - Atherosclerosis
T1  - The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood
VL  - 275
SP  - e206
EP  - e207
DO  - 10.1016/j.atherosclerosis.2018.06.642
ER  - 

@conference{
author = "Đurić, Aleksandar and Čolović, Mirjana B. and Krstić, Danijela Z. and Obrenović, Radmila and Micovic, Z and Đurić, M. and Đurić, Dragan M.",
year = "2018",
abstract = "Aim: Methionine load could lead to hyperhomocysteinemia as well to changes of certain biochemical and inflammation markers. However it is not clear whether other sulfur containing amino acids should affect methionine load effects. The aim was to examine the effects of subchronic methionine (Met) overload on standard biochemical parameters and markers of homocysteine metabolism in rat blood, and to examine whether subchronic administration of N-acetyl-L-cysteine (NAC) affected mentioned parameters.

Methods: The research was conducted during a three-week period (male Wistar albino rats, n=20, body weight of approximately 160g, age of 15-20 days), and the animals were divided into a control and experimental groups, consisted of 10 animals each: a) control group (i.p. 0.1ml/day 0.9%NaCl); b) methionine (0.8 mmol/kg/bw/day); c) methionine (0.8 mmol/kg/bw/day) plus NAC (50 mg/kg/bw/day).

Results: It was found statistically altered levels of certain parameters in NAC plus Met load group vs. control (BUN, CREA, URCA, TBI, TP, ALB, AHDLA, TGL, Na, K, AMY, ACRP, respectively) as well in Met group vs. control. Interestingly, homocysteine level was not increased (control 9.98±0.65 vs. NAC plus Met load 10.21±0.71 μmol/L, P>0.05), but folate was significantly decreased (35.25±2.04 vs. 29.23±1.48 μg/L, P<0.05) as well as vitamin B12 level (882.00±32.00 vs. 756.62±43.84 ng/L, P<0.05). This trend of results was different if it was compared with Met load group vs. control.

Conclusions: The obtained results indicated that monitoring of standard biochemical parameters and homocysteine metabolism markers in blood could provide a valuable insight in the evaluation of NAC plus methionine effects, which may be useful for further research.",
journal = "Atherosclerosis",
title = "The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood",
volume = "275",
pages = "e206-e207",
doi = "10.1016/j.atherosclerosis.2018.06.642"
}

Đurić, A., Čolović, M. B., Krstić, D. Z., Obrenović, R., Micovic, Z., Đurić, M.,& Đurić, D. M.. (2018). The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood. in Atherosclerosis, 275, e206-e207.
https://doi.org/10.1016/j.atherosclerosis.2018.06.642

Đurić A, Čolović MB, Krstić DZ, Obrenović R, Micovic Z, Đurić M, Đurić DM. The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood. in Atherosclerosis. 2018;275:e206-e207.
doi:10.1016/j.atherosclerosis.2018.06.642 .

Đurić, Aleksandar, Čolović, Mirjana B., Krstić, Danijela Z., Obrenović, Radmila, Micovic, Z, Đurić, M., Đurić, Dragan M., "The effects of N-acetyl-L-cysteine on subchronic methionine load in male wistar rats: Focus on standard biochemical parameters and markers of homocysteine metabolism in blood" in Atherosclerosis, 275 (2018):e206-e207,
https://doi.org/10.1016/j.atherosclerosis.2018.06.642 . .

TY  - JOUR
AU  - Stojanović, Marija
AU  - Todorović, Dajana D.
AU  - Šćepanović, Ljiljana
AU  - Mitrović, Dušan M.
AU  - Borozan, Sunčica Z.
AU  - Dragutinović, Vesna V.
AU  - Labudović-Borović, Milica
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s11010-018-3311-2
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8060
AB  - The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.
T2  - Molecular and Cellular Biochemistry
T1  - Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue
VL  - 448
IS  - 1-2
SP  - 43
EP  - 50
DO  - 10.1007/s11010-018-3311-2
ER  - 

@article{
author = "Stojanović, Marija and Todorović, Dajana D. and Šćepanović, Ljiljana and Mitrović, Dušan M. and Borozan, Sunčica Z. and Dragutinović, Vesna V. and Labudović-Borović, Milica and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Dragan M.",
year = "2018",
abstract = "The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.",
journal = "Molecular and Cellular Biochemistry",
title = "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue",
volume = "448",
number = "1-2",
pages = "43-50",
doi = "10.1007/s11010-018-3311-2"
}

Stojanović, M., Todorović, D. D., Šćepanović, L., Mitrović, D. M., Borozan, S. Z., Dragutinović, V. V., Labudović-Borović, M., Krstić, D. Z., Čolović, M. B.,& Đurić, D. M.. (2018). Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry, 448(1-2), 43-50.
https://doi.org/10.1007/s11010-018-3311-2

Stojanović M, Todorović DD, Šćepanović L, Mitrović DM, Borozan SZ, Dragutinović VV, Labudović-Borović M, Krstić DZ, Čolović MB, Đurić DM. Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry. 2018;448(1-2):43-50.
doi:10.1007/s11010-018-3311-2 .

Stojanović, Marija, Todorović, Dajana D., Šćepanović, Ljiljana, Mitrović, Dušan M., Borozan, Sunčica Z., Dragutinović, Vesna V., Labudović-Borović, Milica, Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Dragan M., "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue" in Molecular and Cellular Biochemistry, 448, no. 1-2 (2018):43-50,
https://doi.org/10.1007/s11010-018-3311-2 . .

TY  - CONF
AU  - Đurić, Dragan
AU  - Mićović, Z.
AU  - Stamenković, A.
AU  - Jakovljević Uzelac, J.
AU  - Stojanović, M.
AU  - Šćepanović, Lj.
AU  - Mitrović, D.
AU  - Labudović Borović. M.
AU  - Krstić, Danijela
AU  - Čolović, Mirjana
AU  - Obrenović, R.
AU  - Hadžibegović, A.
AU  - Kostić, S.
AU  - Šobot, T.
AU  - Jakovljević, Vladimir
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12985
C3  - Joint meeting of national physiological societies "New perspectives in physiological research – young investigator forum"
T1  - Comparison of the effects of different sulfur amino acids on cardiovascular system in male wistar rats
SP  - 36
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12985
ER  - 

@conference{
author = "Đurić, Dragan and Mićović, Z. and Stamenković, A. and Jakovljević Uzelac, J. and Stojanović, M. and Šćepanović, Lj. and Mitrović, D. and Labudović Borović. M. and Krstić, Danijela and Čolović, Mirjana and Obrenović, R. and Hadžibegović, A. and Kostić, S. and Šobot, T. and Jakovljević, Vladimir",
year = "2017",
journal = "Joint meeting of national physiological societies "New perspectives in physiological research – young investigator forum"",
title = "Comparison of the effects of different sulfur amino acids on cardiovascular system in male wistar rats",
pages = "36-36",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12985"
}

Đurić, D., Mićović, Z., Stamenković, A., Jakovljević Uzelac, J., Stojanović, M., Šćepanović, Lj., Mitrović, D., Labudović Borović. M., Krstić, D., Čolović, M., Obrenović, R., Hadžibegović, A., Kostić, S., Šobot, T.,& Jakovljević, V.. (2017). Comparison of the effects of different sulfur amino acids on cardiovascular system in male wistar rats. in Joint meeting of national physiological societies "New perspectives in physiological research – young investigator forum", 36-36.
https://hdl.handle.net/21.15107/rcub_vinar_12985

Đurić D, Mićović Z, Stamenković A, Jakovljević Uzelac J, Stojanović M, Šćepanović L, Mitrović D, Labudović Borović. M., Krstić D, Čolović M, Obrenović R, Hadžibegović A, Kostić S, Šobot T, Jakovljević V. Comparison of the effects of different sulfur amino acids on cardiovascular system in male wistar rats. in Joint meeting of national physiological societies "New perspectives in physiological research – young investigator forum". 2017;:36-36.
https://hdl.handle.net/21.15107/rcub_vinar_12985 .

Đurić, Dragan, Mićović, Z., Stamenković, A., Jakovljević Uzelac, J., Stojanović, M., Šćepanović, Lj., Mitrović, D., Labudović Borović. M., Krstić, Danijela, Čolović, Mirjana, Obrenović, R., Hadžibegović, A., Kostić, S., Šobot, T., Jakovljević, Vladimir, "Comparison of the effects of different sulfur amino acids on cardiovascular system in male wistar rats" in Joint meeting of national physiological societies "New perspectives in physiological research – young investigator forum" (2017):36-36,
https://hdl.handle.net/21.15107/rcub_vinar_12985 .

TY  - CONF
AU  - Jakovljević-Uzelac, Jovana
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Đurić, Marko
AU  - Đurić, Dragan M.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7175
C3  - Atherosclerosis
T1  - The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma
VL  - 263
SP  - E137
EP  - E138
DO  - 10.1016/j.atherosclerosis.2017.06.440
ER  - 

@conference{
author = "Jakovljević-Uzelac, Jovana and Čolović, Mirjana B. and Krstić, Danijela Z. and Đurić, Marko and Đurić, Dragan M.",
year = "2017",
journal = "Atherosclerosis",
title = "The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma",
volume = "263",
pages = "E137-E138",
doi = "10.1016/j.atherosclerosis.2017.06.440"
}

Jakovljević-Uzelac, J., Čolović, M. B., Krstić, D. Z., Đurić, M.,& Đurić, D. M.. (2017). The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma. in Atherosclerosis, 263, E137-E138.
https://doi.org/10.1016/j.atherosclerosis.2017.06.440

Jakovljević-Uzelac J, Čolović MB, Krstić DZ, Đurić M, Đurić DM. The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma. in Atherosclerosis. 2017;263:E137-E138.
doi:10.1016/j.atherosclerosis.2017.06.440 .

Jakovljević-Uzelac, Jovana, Čolović, Mirjana B., Krstić, Danijela Z., Đurić, Marko, Đurić, Dragan M., "The Effects of Nitric Oxide Synthase Inhibition Following Acute Administration of Homocysteine on Oxidative Stress Markers in Rat Plasma" in Atherosclerosis, 263 (2017):E137-E138,
https://doi.org/10.1016/j.atherosclerosis.2017.06.440 . .

TY  - CONF
AU  - Hrnčić, Dragan
AU  - Rašić-Marković, Aleksandra
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Šutulović, Nikola
AU  - Grubač, Željko
AU  - Šušić, Veselinka
AU  - Đurić, Dragan M.
AU  - Stanojlović, Olivera
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7176
C3  - Atherosclerosis
T1  - Hyperhomocysteinemia Induced By Methionine Nutritional Overload More Promptly Affects Brain Than Heart Cholinergic System Without Affects on Food Intake and Body Mass Gain
VL  - 263
SP  - E168
EP  - E168
DO  - 10.1016/j.atherosclerosis.2017.06.536
ER  - 

@conference{
author = "Hrnčić, Dragan and Rašić-Marković, Aleksandra and Čolović, Mirjana B. and Krstić, Danijela Z. and Šutulović, Nikola and Grubač, Željko and Šušić, Veselinka and Đurić, Dragan M. and Stanojlović, Olivera",
year = "2017",
journal = "Atherosclerosis",
title = "Hyperhomocysteinemia Induced By Methionine Nutritional Overload More Promptly Affects Brain Than Heart Cholinergic System Without Affects on Food Intake and Body Mass Gain",
volume = "263",
pages = "E168-E168",
doi = "10.1016/j.atherosclerosis.2017.06.536"
}

Hrnčić, D., Rašić-Marković, A., Čolović, M. B., Krstić, D. Z., Šutulović, N., Grubač, Ž., Šušić, V., Đurić, D. M.,& Stanojlović, O.. (2017). Hyperhomocysteinemia Induced By Methionine Nutritional Overload More Promptly Affects Brain Than Heart Cholinergic System Without Affects on Food Intake and Body Mass Gain. in Atherosclerosis, 263, E168-E168.
https://doi.org/10.1016/j.atherosclerosis.2017.06.536

Hrnčić D, Rašić-Marković A, Čolović MB, Krstić DZ, Šutulović N, Grubač Ž, Šušić V, Đurić DM, Stanojlović O. Hyperhomocysteinemia Induced By Methionine Nutritional Overload More Promptly Affects Brain Than Heart Cholinergic System Without Affects on Food Intake and Body Mass Gain. in Atherosclerosis. 2017;263:E168-E168.
doi:10.1016/j.atherosclerosis.2017.06.536 .

Hrnčić, Dragan, Rašić-Marković, Aleksandra, Čolović, Mirjana B., Krstić, Danijela Z., Šutulović, Nikola, Grubač, Željko, Šušić, Veselinka, Đurić, Dragan M., Stanojlović, Olivera, "Hyperhomocysteinemia Induced By Methionine Nutritional Overload More Promptly Affects Brain Than Heart Cholinergic System Without Affects on Food Intake and Body Mass Gain" in Atherosclerosis, 263 (2017):E168-E168,
https://doi.org/10.1016/j.atherosclerosis.2017.06.536 . .

TY  - CONF
AU  - Hrnčić, Dragan
AU  - Rašić-Marković, Aleksandra
AU  - Šutulović, Nikola
AU  - Grubač, Željko
AU  - Vorkapić, M.
AU  - Ademović, A.
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Petrović-Rankov, Bojana
AU  - Šušić, Veselinka
AU  - Đurić, Dragan M.
AU  - Stanojlović, Olivera
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1712
C3  - Acta Physiologica
T1  - Advanced-level analysis of spiking EEG activity potentiated by high dietary methionine: contribution of purinergic signaling
VL  - 221
IS  - Suppl. 713
SP  - 4
EP  - 4
UR  - https://hdl.handle.net/21.15107/rcub_vinar_1712
ER  - 

@conference{
author = "Hrnčić, Dragan and Rašić-Marković, Aleksandra and Šutulović, Nikola and Grubač, Željko and Vorkapić, M. and Ademović, A. and Čolović, Mirjana B. and Krstić, Danijela Z. and Petrović-Rankov, Bojana and Šušić, Veselinka and Đurić, Dragan M. and Stanojlović, Olivera",
year = "2017",
journal = "Acta Physiologica",
title = "Advanced-level analysis of spiking EEG activity potentiated by high dietary methionine: contribution of purinergic signaling",
volume = "221",
number = "Suppl. 713",
pages = "4-4",
url = "https://hdl.handle.net/21.15107/rcub_vinar_1712"
}

Hrnčić, D., Rašić-Marković, A., Šutulović, N., Grubač, Ž., Vorkapić, M., Ademović, A., Čolović, M. B., Krstić, D. Z., Petrović-Rankov, B., Šušić, V., Đurić, D. M.,& Stanojlović, O.. (2017). Advanced-level analysis of spiking EEG activity potentiated by high dietary methionine: contribution of purinergic signaling. in Acta Physiologica, 221(Suppl. 713), 4-4.
https://hdl.handle.net/21.15107/rcub_vinar_1712

Hrnčić D, Rašić-Marković A, Šutulović N, Grubač Ž, Vorkapić M, Ademović A, Čolović MB, Krstić DZ, Petrović-Rankov B, Šušić V, Đurić DM, Stanojlović O. Advanced-level analysis of spiking EEG activity potentiated by high dietary methionine: contribution of purinergic signaling. in Acta Physiologica. 2017;221(Suppl. 713):4-4.
https://hdl.handle.net/21.15107/rcub_vinar_1712 .

Hrnčić, Dragan, Rašić-Marković, Aleksandra, Šutulović, Nikola, Grubač, Željko, Vorkapić, M., Ademović, A., Čolović, Mirjana B., Krstić, Danijela Z., Petrović-Rankov, Bojana, Šušić, Veselinka, Đurić, Dragan M., Stanojlović, Olivera, "Advanced-level analysis of spiking EEG activity potentiated by high dietary methionine: contribution of purinergic signaling" in Acta Physiologica, 221, no. Suppl. 713 (2017):4-4,
https://hdl.handle.net/21.15107/rcub_vinar_1712 .

TY  - CONF
AU  - Đurić, Dragan
AU  - Krstić, Danijela
AU  - Čolović, Mirjana
AU  - Jakovljević, Jovana
AU  - Jakovljević, Vladimir
AU  - Tasić, Nebojša
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12983
AB  - L-methionine, the principal sulfur-containing amino acid in proteins, has important roles in cell physiology as an antioxidant, and in the breakdown of fats and heavy metals. Methionine is the precursor of homocysteine, and participates in the methylation and transsulfuration pathways. Elevated total plasma homocysteine (hyperhomocysteinemia) is associated with atherosclerosis, thromboembolic disease and cancer. Whether homocysteine per se or a coincident metabolic abnormality (homocysteine-related compounds, thiolactone metabolites) causes vascular disease is still an open question. Animals with genetic hyperhomocysteinemia have so far not displayed atheromatous lesions. However, when methionine-rich diets are used to induce hyperhomocysteinemia, vascular pathology is often observed. Such studies have not distinguished the effects of excess dietary methionine from those of hyperhomocysteinemia. It is known that high methionine diet, not only red meat for example lamb, beef, pork but also chicken meat can induce cardiovascular dysfunction but the mechanisms are unclear. It has been hypothesized that a diet rich in methionine can malfunction the cardiovascular system in three ways: (1) by augmenting oxidative stress, (2) by inflammatory manifestations, and (3) by matrix/vascular remodeling. However, some evidence indicates that an excess of methionine can be harmful for other systems, and can increase the risk of developing type-2 diabetes, certain types of cancer, brain alterations such as schizophrenia, and memory impairment. However this is still controversial because previous studies suggesting the use of L-methionine as a treatment for depression and other diseases indicate that it might also improve memory (role in brain function). Thus, the direction of our research is to further elucidate mechanisms of cardiovascular and neural effects of homocysteine vs. methionine overload.
PB  - Kragujevac : Medical Faculty
C3  - Serbian Journal of Experimental and Clinical Research
T1  - Methionine versus homocysteine: future directions in cardiovascular research
VL  - 18
IS  - 1
SP  - 39
EP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12983
ER  - 

@conference{
author = "Đurić, Dragan and Krstić, Danijela and Čolović, Mirjana and Jakovljević, Jovana and Jakovljević, Vladimir and Tasić, Nebojša",
year = "2016",
abstract = "L-methionine, the principal sulfur-containing amino acid in proteins, has important roles in cell physiology as an antioxidant, and in the breakdown of fats and heavy metals. Methionine is the precursor of homocysteine, and participates in the methylation and transsulfuration pathways. Elevated total plasma homocysteine (hyperhomocysteinemia) is associated with atherosclerosis, thromboembolic disease and cancer. Whether homocysteine per se or a coincident metabolic abnormality (homocysteine-related compounds, thiolactone metabolites) causes vascular disease is still an open question. Animals with genetic hyperhomocysteinemia have so far not displayed atheromatous lesions. However, when methionine-rich diets are used to induce hyperhomocysteinemia, vascular pathology is often observed. Such studies have not distinguished the effects of excess dietary methionine from those of hyperhomocysteinemia. It is known that high methionine diet, not only red meat for example lamb, beef, pork but also chicken meat can induce cardiovascular dysfunction but the mechanisms are unclear. It has been hypothesized that a diet rich in methionine can malfunction the cardiovascular system in three ways: (1) by augmenting oxidative stress, (2) by inflammatory manifestations, and (3) by matrix/vascular remodeling. However, some evidence indicates that an excess of methionine can be harmful for other systems, and can increase the risk of developing type-2 diabetes, certain types of cancer, brain alterations such as schizophrenia, and memory impairment. However this is still controversial because previous studies suggesting the use of L-methionine as a treatment for depression and other diseases indicate that it might also improve memory (role in brain function). Thus, the direction of our research is to further elucidate mechanisms of cardiovascular and neural effects of homocysteine vs. methionine overload.",
publisher = "Kragujevac : Medical Faculty",
journal = "Serbian Journal of Experimental and Clinical Research",
title = "Methionine versus homocysteine: future directions in cardiovascular research",
volume = "18",
number = "1",
pages = "39-39",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12983"
}

Đurić, D., Krstić, D., Čolović, M., Jakovljević, J., Jakovljević, V.,& Tasić, N.. (2016). Methionine versus homocysteine: future directions in cardiovascular research. in Serbian Journal of Experimental and Clinical Research
Kragujevac : Medical Faculty., 18(1), 39-39.
https://hdl.handle.net/21.15107/rcub_vinar_12983

Đurić D, Krstić D, Čolović M, Jakovljević J, Jakovljević V, Tasić N. Methionine versus homocysteine: future directions in cardiovascular research. in Serbian Journal of Experimental and Clinical Research. 2016;18(1):39-39.
https://hdl.handle.net/21.15107/rcub_vinar_12983 .

Đurić, Dragan, Krstić, Danijela, Čolović, Mirjana, Jakovljević, Jovana, Jakovljević, Vladimir, Tasić, Nebojša, "Methionine versus homocysteine: future directions in cardiovascular research" in Serbian Journal of Experimental and Clinical Research, 18, no. 1 (2016):39-39,
https://hdl.handle.net/21.15107/rcub_vinar_12983 .

TY  - CONF
AU  - Kostić, S.
AU  - Hadžibegović, A.
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
AU  - Đurić, Dragan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13009
C3  - 2nd Joint Meeting of Slovak and Serbian Physiological Societies "Physiology without frontiers" : Book of abstracts
T1  - Effects of acutely applied methionine on plasma oxidative stress markers and AchE activity in rat cardiac and brain tissue
SP  - 25
EP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13009
ER  - 

@conference{
author = "Kostić, S. and Hadžibegović, A. and Čolović, Mirjana and Krstić, Danijela and Đurić, Dragan",
year = "2016",
journal = "2nd Joint Meeting of Slovak and Serbian Physiological Societies "Physiology without frontiers" : Book of abstracts",
title = "Effects of acutely applied methionine on plasma oxidative stress markers and AchE activity in rat cardiac and brain tissue",
pages = "25-25",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13009"
}

Kostić, S., Hadžibegović, A., Čolović, M., Krstić, D.,& Đurić, D.. (2016). Effects of acutely applied methionine on plasma oxidative stress markers and AchE activity in rat cardiac and brain tissue. in 2nd Joint Meeting of Slovak and Serbian Physiological Societies "Physiology without frontiers" : Book of abstracts, 25-25.
https://hdl.handle.net/21.15107/rcub_vinar_13009

Kostić S, Hadžibegović A, Čolović M, Krstić D, Đurić D. Effects of acutely applied methionine on plasma oxidative stress markers and AchE activity in rat cardiac and brain tissue. in 2nd Joint Meeting of Slovak and Serbian Physiological Societies "Physiology without frontiers" : Book of abstracts. 2016;:25-25.
https://hdl.handle.net/21.15107/rcub_vinar_13009 .

Kostić, S., Hadžibegović, A., Čolović, Mirjana, Krstić, Danijela, Đurić, Dragan, "Effects of acutely applied methionine on plasma oxidative stress markers and AchE activity in rat cardiac and brain tissue" in 2nd Joint Meeting of Slovak and Serbian Physiological Societies "Physiology without frontiers" : Book of abstracts (2016):25-25,
https://hdl.handle.net/21.15107/rcub_vinar_13009 .

TY  - JOUR
AU  - Rašić-Marković, Aleksandra
AU  - Hrnčić, Dragan
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Djuric, E.
AU  - Rankov-Petrovic, B.
AU  - Šušić, Veselinka
AU  - Stanojlović, Olivera
AU  - Đurić, Dragan M.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7114
AB  - The aim of the present study was to examine the effect of subchronic co-administration of folic acid (F) and L-arginine (A) on behavioural and electroencephalographic (EEG) characteristics of DL homocysteine thiolactone (H) induced seizures in adult rats. The activity of membrane ATPases in different brain regions were also investigated. Rats were treated with F, A, or vehicle for 15 days (regimen: F 5 mg/kg + A 500 mg/kg (F5A500); F 10 mg/kg + A 300 mg/kg (F10A300)). Seizures were elicited by convulsive dose of H (H, F5A500H, F10A300H) Subchronic supplementation with F and A did not affect seizure incidence, number of seizure episodes, and severity in F5A500H and F10A300H groups vs. H group. However, a tendency to increase latency and decrease the number of seizure episodes was noticed in the F10A300H group. EEG mean spectral power densities during ictal periods were significantly lower in F10A300H vs. H group. The activity of Na+/K+-ATPase and Mg2+-ATPase was significantly increased in almost all examined structures in rats treated with F and A. We can conclude that subchronic supplementation with folic acid and L-arginine has an antiepileptic effect in DL homocysteine thiolactone induced epilepsy.
T2  - Canadian Journal of Physiology and Pharmacology
T1  - The effect of subchronic supplementation with folic acid and L-arginine on homocysteine-induced seizures
VL  - 94
IS  - 10
SP  - 1083
EP  - 1089
DO  - 10.1139/cjpp-2016-0076
ER  - 

@article{
author = "Rašić-Marković, Aleksandra and Hrnčić, Dragan and Krstić, Danijela Z. and Čolović, Mirjana B. and Djuric, E. and Rankov-Petrovic, B. and Šušić, Veselinka and Stanojlović, Olivera and Đurić, Dragan M.",
year = "2016",
abstract = "The aim of the present study was to examine the effect of subchronic co-administration of folic acid (F) and L-arginine (A) on behavioural and electroencephalographic (EEG) characteristics of DL homocysteine thiolactone (H) induced seizures in adult rats. The activity of membrane ATPases in different brain regions were also investigated. Rats were treated with F, A, or vehicle for 15 days (regimen: F 5 mg/kg + A 500 mg/kg (F5A500); F 10 mg/kg + A 300 mg/kg (F10A300)). Seizures were elicited by convulsive dose of H (H, F5A500H, F10A300H) Subchronic supplementation with F and A did not affect seizure incidence, number of seizure episodes, and severity in F5A500H and F10A300H groups vs. H group. However, a tendency to increase latency and decrease the number of seizure episodes was noticed in the F10A300H group. EEG mean spectral power densities during ictal periods were significantly lower in F10A300H vs. H group. The activity of Na+/K+-ATPase and Mg2+-ATPase was significantly increased in almost all examined structures in rats treated with F and A. We can conclude that subchronic supplementation with folic acid and L-arginine has an antiepileptic effect in DL homocysteine thiolactone induced epilepsy.",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "The effect of subchronic supplementation with folic acid and L-arginine on homocysteine-induced seizures",
volume = "94",
number = "10",
pages = "1083-1089",
doi = "10.1139/cjpp-2016-0076"
}

Rašić-Marković, A., Hrnčić, D., Krstić, D. Z., Čolović, M. B., Djuric, E., Rankov-Petrovic, B., Šušić, V., Stanojlović, O.,& Đurić, D. M.. (2016). The effect of subchronic supplementation with folic acid and L-arginine on homocysteine-induced seizures. in Canadian Journal of Physiology and Pharmacology, 94(10), 1083-1089.
https://doi.org/10.1139/cjpp-2016-0076

Rašić-Marković A, Hrnčić D, Krstić DZ, Čolović MB, Djuric E, Rankov-Petrovic B, Šušić V, Stanojlović O, Đurić DM. The effect of subchronic supplementation with folic acid and L-arginine on homocysteine-induced seizures. in Canadian Journal of Physiology and Pharmacology. 2016;94(10):1083-1089.
doi:10.1139/cjpp-2016-0076 .

Rašić-Marković, Aleksandra, Hrnčić, Dragan, Krstić, Danijela Z., Čolović, Mirjana B., Djuric, E., Rankov-Petrovic, B., Šušić, Veselinka, Stanojlović, Olivera, Đurić, Dragan M., "The effect of subchronic supplementation with folic acid and L-arginine on homocysteine-induced seizures" in Canadian Journal of Physiology and Pharmacology, 94, no. 10 (2016):1083-1089,
https://doi.org/10.1139/cjpp-2016-0076 . .

TY  - JOUR
AU  - Stojanović, Marija
AU  - Scepanovic, Ljiljana
AU  - Bosnic, Olivera
AU  - Mitrovic, Dusan
AU  - Jozanov-Stankov, Olga
AU  - Scepanovic, Vuk
AU  - Scepanovic, Radomir
AU  - Stojanovic, Teja
AU  - Ilic, Slobodan
AU  - Đurić, Dragan M.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1117
AB  - Oxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chrons disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hour incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 mu mol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1: 10). Homogenates were centrifuged at 10000 for 10 min at 4 degrees C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p LT 0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p LT 0.001. Exposition to high D, L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p LT 0.01). Homocysteine also modified the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p LT 0.001). Homocysteine induced a consistent oxidative stress in rats intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine.
T2  - Acta Veterinaria, Beograd
T1  - Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver
VL  - 66
IS  - 1
SP  - 26
EP  - 36
DO  - 10.1515/acve-2016-0002
ER  - 

@article{
author = "Stojanović, Marija and Scepanovic, Ljiljana and Bosnic, Olivera and Mitrovic, Dusan and Jozanov-Stankov, Olga and Scepanovic, Vuk and Scepanovic, Radomir and Stojanovic, Teja and Ilic, Slobodan and Đurić, Dragan M.",
year = "2016",
abstract = "Oxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chrons disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hour incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 mu mol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1: 10). Homogenates were centrifuged at 10000 for 10 min at 4 degrees C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p LT 0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p LT 0.001. Exposition to high D, L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p LT 0.01). Homocysteine also modified the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p LT 0.001). Homocysteine induced a consistent oxidative stress in rats intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine.",
journal = "Acta Veterinaria, Beograd",
title = "Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver",
volume = "66",
number = "1",
pages = "26-36",
doi = "10.1515/acve-2016-0002"
}

Stojanović, M., Scepanovic, L., Bosnic, O., Mitrovic, D., Jozanov-Stankov, O., Scepanovic, V., Scepanovic, R., Stojanovic, T., Ilic, S.,& Đurić, D. M.. (2016). Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver. in Acta Veterinaria, Beograd, 66(1), 26-36.
https://doi.org/10.1515/acve-2016-0002

Stojanović M, Scepanovic L, Bosnic O, Mitrovic D, Jozanov-Stankov O, Scepanovic V, Scepanovic R, Stojanovic T, Ilic S, Đurić DM. Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver. in Acta Veterinaria, Beograd. 2016;66(1):26-36.
doi:10.1515/acve-2016-0002 .

Stojanović, Marija, Scepanovic, Ljiljana, Bosnic, Olivera, Mitrovic, Dusan, Jozanov-Stankov, Olga, Scepanovic, Vuk, Scepanovic, Radomir, Stojanovic, Teja, Ilic, Slobodan, Đurić, Dragan M., "Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver" in Acta Veterinaria, Beograd, 66, no. 1 (2016):26-36,
https://doi.org/10.1515/acve-2016-0002 . .

TY  - JOUR
AU  - Rašić-Marković, Aleksandra
AU  - Rankov-Petrović, B.
AU  - Hrnčić, Dragan
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Macut, Dj
AU  - Đurić, Dragan M.
AU  - Stanojlović, Olivera
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/619
AB  - Influence of folic acid on the CNS is still unclear. Folate has a neuroprotective effect, while on the other hand excess folate can exacerbate seizures in epileptics. The aim of the present study was to examine the effect of subchronic administration of folic acid on behavioural and electroencephalographic (EEG) characteristics of DL homocysteine thiolactone induced seizures in adult rats. The activity of Na+/K+-ATPase and Mg2+-ATPase in different brain regions was investigated. Adult male Wistar rats were divided into groups: 1. Controls (C, 0.9% NaCl); 2. DL homocysteine-thiolactone 8.0 mmol/kg (H); 3. Subchronic supplementation with folic acid 5 mg/kg for 7 days (F) and 4. Subchronic supplementation with F + single dose of H (FH). Seizure behaviour was assessed by incidence, latency, number and intensity of seizure episodes. Seizure severity was described by a descriptive scale with grades 0-4. For EEG recordings, three gold-plated recording electrodes were implanted into the skull. Subchronic supplementation with folic acid did not affect seizure incidence, median number of seizure episodes and severity in FH, comparison with H (p GT 0.05). The majority of seizure episodes in all groups were of grade 2. There were no significant differences in lethal outcomes at 24 h upon H injection in the FH vs. H group. The activity of Na+/K+-ATPase and Mg2+-ATPase was significantly increased in almost all examined structures in the FH vs. H group. Subchronic folic acid administration did not exacerbate H induced seizures and completely recovered the activity of ATPases.
T2  - Acta Physiologica Hungarica
T1  - The effect of subchronic supplementation with folic acid on homocysteine induced seizures
VL  - 102
IS  - 2
SP  - 151
EP  - 162
DO  - 10.1556/036.102.2015.2.6
ER  - 

@article{
author = "Rašić-Marković, Aleksandra and Rankov-Petrović, B. and Hrnčić, Dragan and Krstić, Danijela Z. and Čolović, Mirjana B. and Macut, Dj and Đurić, Dragan M. and Stanojlović, Olivera",
year = "2015",
abstract = "Influence of folic acid on the CNS is still unclear. Folate has a neuroprotective effect, while on the other hand excess folate can exacerbate seizures in epileptics. The aim of the present study was to examine the effect of subchronic administration of folic acid on behavioural and electroencephalographic (EEG) characteristics of DL homocysteine thiolactone induced seizures in adult rats. The activity of Na+/K+-ATPase and Mg2+-ATPase in different brain regions was investigated. Adult male Wistar rats were divided into groups: 1. Controls (C, 0.9% NaCl); 2. DL homocysteine-thiolactone 8.0 mmol/kg (H); 3. Subchronic supplementation with folic acid 5 mg/kg for 7 days (F) and 4. Subchronic supplementation with F + single dose of H (FH). Seizure behaviour was assessed by incidence, latency, number and intensity of seizure episodes. Seizure severity was described by a descriptive scale with grades 0-4. For EEG recordings, three gold-plated recording electrodes were implanted into the skull. Subchronic supplementation with folic acid did not affect seizure incidence, median number of seizure episodes and severity in FH, comparison with H (p GT 0.05). The majority of seizure episodes in all groups were of grade 2. There were no significant differences in lethal outcomes at 24 h upon H injection in the FH vs. H group. The activity of Na+/K+-ATPase and Mg2+-ATPase was significantly increased in almost all examined structures in the FH vs. H group. Subchronic folic acid administration did not exacerbate H induced seizures and completely recovered the activity of ATPases.",
journal = "Acta Physiologica Hungarica",
title = "The effect of subchronic supplementation with folic acid on homocysteine induced seizures",
volume = "102",
number = "2",
pages = "151-162",
doi = "10.1556/036.102.2015.2.6"
}

Rašić-Marković, A., Rankov-Petrović, B., Hrnčić, D., Krstić, D. Z., Čolović, M. B., Macut, D., Đurić, D. M.,& Stanojlović, O.. (2015). The effect of subchronic supplementation with folic acid on homocysteine induced seizures. in Acta Physiologica Hungarica, 102(2), 151-162.
https://doi.org/10.1556/036.102.2015.2.6

Rašić-Marković A, Rankov-Petrović B, Hrnčić D, Krstić DZ, Čolović MB, Macut D, Đurić DM, Stanojlović O. The effect of subchronic supplementation with folic acid on homocysteine induced seizures. in Acta Physiologica Hungarica. 2015;102(2):151-162.
doi:10.1556/036.102.2015.2.6 .

Rašić-Marković, Aleksandra, Rankov-Petrović, B., Hrnčić, Dragan, Krstić, Danijela Z., Čolović, Mirjana B., Macut, Dj, Đurić, Dragan M., Stanojlović, Olivera, "The effect of subchronic supplementation with folic acid on homocysteine induced seizures" in Acta Physiologica Hungarica, 102, no. 2 (2015):151-162,
https://doi.org/10.1556/036.102.2015.2.6 . .

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Vasić, Vesna M.
AU  - Avramović, Nataša
AU  - Gajic, Milan M.
AU  - Đurić, Dragan M.
AU  - Krstić, Danijela Z.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/409
AB  - Although primary toxic action of organophosphorous insecticides is associated with acetylcholinesterase inhibition, later studies suggest that oxidative stress may be responsible for induced organophosphates toxicity. These studies mostly include thio forms, while the effects of their metabolites/ degradation products have been less investigated. Therefore, this paper studies the toxic effects of diazinon degradation products, diazoxon and 2-isopropyl-6-methyl-4-pyrimidinol, and compares them with the toxic potential of the parent compound. The toxicity induced by various concentrations of the investigated compounds was in vitro evaluated by the activities of acetylcholinesterase, ATPases, antioxidant defense enzymes and lactate dehydrogenase, and malondialdehyde level in rat brain synaptosomes. Diazinon inhibited acetylcholinesterase and Na+/K+-ATPase in dose-dependent manner, while the inhibition of ecto-ATPase activity was less than 15% at all investigated concentrations. It did not demonstrate noteworthy prooxidative properties causing increase (up to 10%) in antioxidant enzymes activity and malondialdehyde level, as a marker of lipid peroxidation. Diazinon oxidation product, diazoxon was found as the most toxic investigated compound. Beside the expected strong inhibitory effect on acetylcholinesterase, it induced dose-dependent and almost complete inhibition of Na+/K+-ATPase and ecto-ATPase at the highest investigated concentration (0.1 mM). Increasing diazoxon concentrations activated catalase (up to 30%), superoxide dismutase (up to 50%), glutathione peroxidase (up to 30%), and significantly increased malondialdehyde level (up to 50%). The investigated hydrolysis product of diazinon, 2-isopropyl-6-methyl-4-pyrimidinol did not remarkably alter the activities of acetylcholinesterase, Na+/K+-ATPase, catalase, glutathione peroxidase and lipid peroxidation level (up to about 10%). Although this diazinon metabolite has been known as non toxic, it induced superoxide dismutase stimulation up to 30%. Finally, even high concentrations of both diazinon and its metabolites did noticeably affect lactate dehydrogenase activity as a marker of synaptosomal integrity. The changes in investigated biochemical parameters in rat brain synaptosomes could serve as indicators of toxicity due to the exposure to thio organophosphates and/or their break-down products. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
T2  - Toxicology Letters
T1  - In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes
VL  - 233
IS  - 1
SP  - 29
EP  - 37
DO  - 10.1016/j.toxlet.2015.01.003
ER  - 

@article{
author = "Čolović, Mirjana B. and Vasić, Vesna M. and Avramović, Nataša and Gajic, Milan M. and Đurić, Dragan M. and Krstić, Danijela Z.",
year = "2015",
abstract = "Although primary toxic action of organophosphorous insecticides is associated with acetylcholinesterase inhibition, later studies suggest that oxidative stress may be responsible for induced organophosphates toxicity. These studies mostly include thio forms, while the effects of their metabolites/ degradation products have been less investigated. Therefore, this paper studies the toxic effects of diazinon degradation products, diazoxon and 2-isopropyl-6-methyl-4-pyrimidinol, and compares them with the toxic potential of the parent compound. The toxicity induced by various concentrations of the investigated compounds was in vitro evaluated by the activities of acetylcholinesterase, ATPases, antioxidant defense enzymes and lactate dehydrogenase, and malondialdehyde level in rat brain synaptosomes. Diazinon inhibited acetylcholinesterase and Na+/K+-ATPase in dose-dependent manner, while the inhibition of ecto-ATPase activity was less than 15% at all investigated concentrations. It did not demonstrate noteworthy prooxidative properties causing increase (up to 10%) in antioxidant enzymes activity and malondialdehyde level, as a marker of lipid peroxidation. Diazinon oxidation product, diazoxon was found as the most toxic investigated compound. Beside the expected strong inhibitory effect on acetylcholinesterase, it induced dose-dependent and almost complete inhibition of Na+/K+-ATPase and ecto-ATPase at the highest investigated concentration (0.1 mM). Increasing diazoxon concentrations activated catalase (up to 30%), superoxide dismutase (up to 50%), glutathione peroxidase (up to 30%), and significantly increased malondialdehyde level (up to 50%). The investigated hydrolysis product of diazinon, 2-isopropyl-6-methyl-4-pyrimidinol did not remarkably alter the activities of acetylcholinesterase, Na+/K+-ATPase, catalase, glutathione peroxidase and lipid peroxidation level (up to about 10%). Although this diazinon metabolite has been known as non toxic, it induced superoxide dismutase stimulation up to 30%. Finally, even high concentrations of both diazinon and its metabolites did noticeably affect lactate dehydrogenase activity as a marker of synaptosomal integrity. The changes in investigated biochemical parameters in rat brain synaptosomes could serve as indicators of toxicity due to the exposure to thio organophosphates and/or their break-down products. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
journal = "Toxicology Letters",
title = "In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes",
volume = "233",
number = "1",
pages = "29-37",
doi = "10.1016/j.toxlet.2015.01.003"
}

Čolović, M. B., Vasić, V. M., Avramović, N., Gajic, M. M., Đurić, D. M.,& Krstić, D. Z.. (2015). In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes. in Toxicology Letters, 233(1), 29-37.
https://doi.org/10.1016/j.toxlet.2015.01.003

Čolović MB, Vasić VM, Avramović N, Gajic MM, Đurić DM, Krstić DZ. In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes. in Toxicology Letters. 2015;233(1):29-37.
doi:10.1016/j.toxlet.2015.01.003 .

Čolović, Mirjana B., Vasić, Vesna M., Avramović, Nataša, Gajic, Milan M., Đurić, Dragan M., Krstić, Danijela Z., "In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes" in Toxicology Letters, 233, no. 1 (2015):29-37,
https://doi.org/10.1016/j.toxlet.2015.01.003 . .

TY  - JOUR
AU  - Hrnčić, Dragan
AU  - Rašić-Marković, Aleksandra
AU  - Lekovic, J.
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Macut, D.
AU  - Šušić, Veselinka
AU  - Đurić, Dragan M.
AU  - Stanojlović, Olivera
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6049
AB  - The aim of the study was to examine the effects of chronic exercise training on seizures induced by homocysteine thiolactone (HCT) in adult rats. Rats were assigned to: sedentary control; exercise control; sedentary +HCT; exercise + HCT group. Animals in the exercise groups ran 30 min daily on a treadmill for 30 consecutive days (belt speed 20 m/min), while sedentary rats spent the same time on the treadmill (speed 0 m/min). On the 31st day, the HCT groups received HCT (8.0 mmol/kg), while the control groups received vehicle. Afterwards, convulsive behavior and EEG activity were registered. Lipid peroxidation, superoxide dismutase (SOD) and catalase (CAT) activity were ascertained in the rat hippocampus. No signs of seizures were registered in sedentary and exercise control rats. Seizure latency was increased, while number of seizure episodes and spike-and-wave discharges (SWD) in EEG were decreased in the exercise + HCT compared to the sedentary +HCT group. Seizure incidence, the severity thereof and duration of SWDs were not significantly different between these groups. Exercise partly prevented increase of lipid peroxidation and decrease of the SOD and CAT activity after HCT administration. These results indicate beneficial effects of exercise in model of HCT induced seizures in rats, what could be, at least in part, a consequence of improved antioxidant enzymes activity.
T2  - International Journal of Sports Medicine
T1  - Exercise Decreases Susceptibility to Homocysteine Seizures: the Role of Oxidative Stress
VL  - 35
IS  - 7
SP  - 544
EP  - 550
DO  - 10.1055/s-0033-1357162
ER  - 

@article{
author = "Hrnčić, Dragan and Rašić-Marković, Aleksandra and Lekovic, J. and Krstić, Danijela Z. and Čolović, Mirjana B. and Macut, D. and Šušić, Veselinka and Đurić, Dragan M. and Stanojlović, Olivera",
year = "2014",
abstract = "The aim of the study was to examine the effects of chronic exercise training on seizures induced by homocysteine thiolactone (HCT) in adult rats. Rats were assigned to: sedentary control; exercise control; sedentary +HCT; exercise + HCT group. Animals in the exercise groups ran 30 min daily on a treadmill for 30 consecutive days (belt speed 20 m/min), while sedentary rats spent the same time on the treadmill (speed 0 m/min). On the 31st day, the HCT groups received HCT (8.0 mmol/kg), while the control groups received vehicle. Afterwards, convulsive behavior and EEG activity were registered. Lipid peroxidation, superoxide dismutase (SOD) and catalase (CAT) activity were ascertained in the rat hippocampus. No signs of seizures were registered in sedentary and exercise control rats. Seizure latency was increased, while number of seizure episodes and spike-and-wave discharges (SWD) in EEG were decreased in the exercise + HCT compared to the sedentary +HCT group. Seizure incidence, the severity thereof and duration of SWDs were not significantly different between these groups. Exercise partly prevented increase of lipid peroxidation and decrease of the SOD and CAT activity after HCT administration. These results indicate beneficial effects of exercise in model of HCT induced seizures in rats, what could be, at least in part, a consequence of improved antioxidant enzymes activity.",
journal = "International Journal of Sports Medicine",
title = "Exercise Decreases Susceptibility to Homocysteine Seizures: the Role of Oxidative Stress",
volume = "35",
number = "7",
pages = "544-550",
doi = "10.1055/s-0033-1357162"
}

Hrnčić, D., Rašić-Marković, A., Lekovic, J., Krstić, D. Z., Čolović, M. B., Macut, D., Šušić, V., Đurić, D. M.,& Stanojlović, O.. (2014). Exercise Decreases Susceptibility to Homocysteine Seizures: the Role of Oxidative Stress. in International Journal of Sports Medicine, 35(7), 544-550.
https://doi.org/10.1055/s-0033-1357162

Hrnčić D, Rašić-Marković A, Lekovic J, Krstić DZ, Čolović MB, Macut D, Šušić V, Đurić DM, Stanojlović O. Exercise Decreases Susceptibility to Homocysteine Seizures: the Role of Oxidative Stress. in International Journal of Sports Medicine. 2014;35(7):544-550.
doi:10.1055/s-0033-1357162 .

Hrnčić, Dragan, Rašić-Marković, Aleksandra, Lekovic, J., Krstić, Danijela Z., Čolović, Mirjana B., Macut, D., Šušić, Veselinka, Đurić, Dragan M., Stanojlović, Olivera, "Exercise Decreases Susceptibility to Homocysteine Seizures: the Role of Oxidative Stress" in International Journal of Sports Medicine, 35, no. 7 (2014):544-550,
https://doi.org/10.1055/s-0033-1357162 . .

TY  - CONF
AU  - Đurić, Dragan M.
AU  - Stanojlović, Olivera
AU  - Hrnčić, Dragan
AU  - Puškaš, Nela
AU  - Rašić-Marković, Aleksandra
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Bjekić, J. M.
AU  - Grubač, Željko
AU  - Šutulović, Nikola
AU  - Šušić, Veselinka
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/72
PB  - Wiley
C3  - Acta Physiologica
T1  - Effects of methionine-enriched diet on the rat heart and aorta
VL  - 211
IS  - SI
SP  - 78
EP  - 78
DO  - 10.1111/apha.12362
ER  - 

@conference{
author = "Đurić, Dragan M. and Stanojlović, Olivera and Hrnčić, Dragan and Puškaš, Nela and Rašić-Marković, Aleksandra and Čolović, Mirjana B. and Krstić, Danijela Z. and Bjekić, J. M. and Grubač, Željko and Šutulović, Nikola and Šušić, Veselinka",
year = "2014",
publisher = "Wiley",
journal = "Acta Physiologica",
title = "Effects of methionine-enriched diet on the rat heart and aorta",
volume = "211",
number = "SI",
pages = "78-78",
doi = "10.1111/apha.12362"
}

Đurić, D. M., Stanojlović, O., Hrnčić, D., Puškaš, N., Rašić-Marković, A., Čolović, M. B., Krstić, D. Z., Bjekić, J. M., Grubač, Ž., Šutulović, N.,& Šušić, V.. (2014). Effects of methionine-enriched diet on the rat heart and aorta. in Acta Physiologica
Wiley., 211(SI), 78-78.
https://doi.org/10.1111/apha.12362

Đurić DM, Stanojlović O, Hrnčić D, Puškaš N, Rašić-Marković A, Čolović MB, Krstić DZ, Bjekić JM, Grubač Ž, Šutulović N, Šušić V. Effects of methionine-enriched diet on the rat heart and aorta. in Acta Physiologica. 2014;211(SI):78-78.
doi:10.1111/apha.12362 .

Đurić, Dragan M., Stanojlović, Olivera, Hrnčić, Dragan, Puškaš, Nela, Rašić-Marković, Aleksandra, Čolović, Mirjana B., Krstić, Danijela Z., Bjekić, J. M., Grubač, Željko, Šutulović, Nikola, Šušić, Veselinka, "Effects of methionine-enriched diet on the rat heart and aorta" in Acta Physiologica, 211, no. SI (2014):78-78,
https://doi.org/10.1111/apha.12362 . .

TY  - JOUR
AU  - Rašić-Marković, Aleksandra
AU  - Stanojlović, Olivera
AU  - Hrnčić, Dragan
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Šušić, Veselinka
AU  - Radosavljevic, Tatjana
AU  - Đurić, Dragan M.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3709
AB  - Hyperhomocysteinemia is associated with various pathologies including cardiovascular disease, stroke, and cognitive dysfunctions. Systemic administration of homocysteine can trigger seizures in animals, and patients with homocystinuria suffer from epileptic seizures. Available data suggest that homocysteine can be harmful to human cells because of its metabolic conversion to homocysteine thiolactone, a reactive thioester. A number of reports have demonstrated a reduction of Na+/K+-ATPase activity in cerebral ischemia, epilepsy and neurodegeneration possibly associated with excitotoxic mechanisms. The aim of this study was to examine the in vivo effects of d,l-homocysteine and d,l-homocysteine thiolactone on Na+/K+- and Mg2+-ATPase activities in erythrocyte (RBC), brain cortex, hippocampus, and brain stem of adult male rats. Our results demonstrate a moderate inhibition of rat hippocampal Na+/K+-ATPase activity by d,l-homocysteine, which however expressed no effect on the activity of this enzyme in the cortex and brain stem. In contrast,d,l-homocysteine thiolactone strongly inhibited Na+/K+-ATPase activity in cortex, hippocampus and brain stem of rats. RBC Na+/K+-ATPase and Mg2+-ATPase activities were not affected by d,l-homocysteine, while d,l-homocysteine thiolactone inhibited only Na+/K+-ATPase activity. This study results show that homocysteine thiolactone significantly inhibits Na+/K+-ATPase activity in the cortex, hippocampus, and brain stem, which may contribute at least in part to the understanding of excitotoxic and convulsive properties of this substance.
T2  - Molecular and Cellular Biochemistry
T1  - The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration
VL  - 327
IS  - 1-2
SP  - 39
EP  - 45
DO  - 10.1007/s11010-009-0040-6
ER  - 

@article{
author = "Rašić-Marković, Aleksandra and Stanojlović, Olivera and Hrnčić, Dragan and Krstić, Danijela Z. and Čolović, Mirjana B. and Šušić, Veselinka and Radosavljevic, Tatjana and Đurić, Dragan M.",
year = "2009",
abstract = "Hyperhomocysteinemia is associated with various pathologies including cardiovascular disease, stroke, and cognitive dysfunctions. Systemic administration of homocysteine can trigger seizures in animals, and patients with homocystinuria suffer from epileptic seizures. Available data suggest that homocysteine can be harmful to human cells because of its metabolic conversion to homocysteine thiolactone, a reactive thioester. A number of reports have demonstrated a reduction of Na+/K+-ATPase activity in cerebral ischemia, epilepsy and neurodegeneration possibly associated with excitotoxic mechanisms. The aim of this study was to examine the in vivo effects of d,l-homocysteine and d,l-homocysteine thiolactone on Na+/K+- and Mg2+-ATPase activities in erythrocyte (RBC), brain cortex, hippocampus, and brain stem of adult male rats. Our results demonstrate a moderate inhibition of rat hippocampal Na+/K+-ATPase activity by d,l-homocysteine, which however expressed no effect on the activity of this enzyme in the cortex and brain stem. In contrast,d,l-homocysteine thiolactone strongly inhibited Na+/K+-ATPase activity in cortex, hippocampus and brain stem of rats. RBC Na+/K+-ATPase and Mg2+-ATPase activities were not affected by d,l-homocysteine, while d,l-homocysteine thiolactone inhibited only Na+/K+-ATPase activity. This study results show that homocysteine thiolactone significantly inhibits Na+/K+-ATPase activity in the cortex, hippocampus, and brain stem, which may contribute at least in part to the understanding of excitotoxic and convulsive properties of this substance.",
journal = "Molecular and Cellular Biochemistry",
title = "The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration",
volume = "327",
number = "1-2",
pages = "39-45",
doi = "10.1007/s11010-009-0040-6"
}

Rašić-Marković, A., Stanojlović, O., Hrnčić, D., Krstić, D. Z., Čolović, M. B., Šušić, V., Radosavljevic, T.,& Đurić, D. M.. (2009). The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration. in Molecular and Cellular Biochemistry, 327(1-2), 39-45.
https://doi.org/10.1007/s11010-009-0040-6

Rašić-Marković A, Stanojlović O, Hrnčić D, Krstić DZ, Čolović MB, Šušić V, Radosavljevic T, Đurić DM. The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration. in Molecular and Cellular Biochemistry. 2009;327(1-2):39-45.
doi:10.1007/s11010-009-0040-6 .

Rašić-Marković, Aleksandra, Stanojlović, Olivera, Hrnčić, Dragan, Krstić, Danijela Z., Čolović, Mirjana B., Šušić, Veselinka, Radosavljevic, Tatjana, Đurić, Dragan M., "The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration" in Molecular and Cellular Biochemistry, 327, no. 1-2 (2009):39-45,
https://doi.org/10.1007/s11010-009-0040-6 . .

TY  - JOUR
AU  - Rašić-Marković, Aleksandra
AU  - Đurić, Dragan M.
AU  - Hrnčić, Dragan
AU  - Lončar-Stevanović, Helena
AU  - Vucevic, Danijela
AU  - Mladenovic, Dusan
AU  - Brkić, Predrag
AU  - Djuro, Macut
AU  - Stanojević, Ivana
AU  - Stanojlović, Olivera
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2729
AB  - The effects of ethanol on epilepsy are very complex. Ethanol can have depressant as well as excitatory effect on different animal models of epilepsy. Systemic administration of homocysteine can trigger seizures. The aim of the present study was to examine the changes of total spectral power density after ethanol alone and together with homocysteine thiolactone in adult rats. Adult male Wistar rats were divided into following groups: I. saline-injected, (control) C; 2. D, L-homocysteine thiolactone, H (8 mmol/kg); 3. ethanol, E (E(0.5), 0.5 g/kg; E(1), 1 g/kg; E(2), 2 g/kg) and 4. E (E(0.5), E(1), and E(2)) 30 min prior to H, EH (E(0.5)H, E(1)H and E(2)H). For EEG recordings three gold-plated screws were implanted into the skull. Our results demonstrate that ethanol, when applied alone, increased total EEG spectral power density of adult rats with a marked spectrum shift toward low frequency waves. In EH groups, increasing doses of ethanol exhibited a dose-dependent effect upon spectral power density. Ethanol increased EEG spectral power density in E(0.5)H and E(1)H group, comparing to the H group (p GT 0.05), the maximal increase was recorded with the lowest ethanol dose applied. The highest dose of ethanol (E(2)H) significantly decreased total power spectra density, comparing to the H group. We can conclude that high doses of ethanol depressed marked increase in EEG power spectrum induced by D,L-homocysteine thiolactone.
T2  - General Physiology and Biophysics
T1  - High dose of ethanol decreases total spectral power density in seizures induced by D,L-homocysteine thiolactone in adult rats
VL  - 28
IS  - SI
SP  - 25
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2729
ER  - 

@article{
author = "Rašić-Marković, Aleksandra and Đurić, Dragan M. and Hrnčić, Dragan and Lončar-Stevanović, Helena and Vucevic, Danijela and Mladenovic, Dusan and Brkić, Predrag and Djuro, Macut and Stanojević, Ivana and Stanojlović, Olivera",
year = "2009",
abstract = "The effects of ethanol on epilepsy are very complex. Ethanol can have depressant as well as excitatory effect on different animal models of epilepsy. Systemic administration of homocysteine can trigger seizures. The aim of the present study was to examine the changes of total spectral power density after ethanol alone and together with homocysteine thiolactone in adult rats. Adult male Wistar rats were divided into following groups: I. saline-injected, (control) C; 2. D, L-homocysteine thiolactone, H (8 mmol/kg); 3. ethanol, E (E(0.5), 0.5 g/kg; E(1), 1 g/kg; E(2), 2 g/kg) and 4. E (E(0.5), E(1), and E(2)) 30 min prior to H, EH (E(0.5)H, E(1)H and E(2)H). For EEG recordings three gold-plated screws were implanted into the skull. Our results demonstrate that ethanol, when applied alone, increased total EEG spectral power density of adult rats with a marked spectrum shift toward low frequency waves. In EH groups, increasing doses of ethanol exhibited a dose-dependent effect upon spectral power density. Ethanol increased EEG spectral power density in E(0.5)H and E(1)H group, comparing to the H group (p GT 0.05), the maximal increase was recorded with the lowest ethanol dose applied. The highest dose of ethanol (E(2)H) significantly decreased total power spectra density, comparing to the H group. We can conclude that high doses of ethanol depressed marked increase in EEG power spectrum induced by D,L-homocysteine thiolactone.",
journal = "General Physiology and Biophysics",
title = "High dose of ethanol decreases total spectral power density in seizures induced by D,L-homocysteine thiolactone in adult rats",
volume = "28",
number = "SI",
pages = "25-32",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2729"
}

Rašić-Marković, A., Đurić, D. M., Hrnčić, D., Lončar-Stevanović, H., Vucevic, D., Mladenovic, D., Brkić, P., Djuro, M., Stanojević, I.,& Stanojlović, O.. (2009). High dose of ethanol decreases total spectral power density in seizures induced by D,L-homocysteine thiolactone in adult rats. in General Physiology and Biophysics, 28(SI), 25-32.
https://hdl.handle.net/21.15107/rcub_vinar_2729

Rašić-Marković A, Đurić DM, Hrnčić D, Lončar-Stevanović H, Vucevic D, Mladenovic D, Brkić P, Djuro M, Stanojević I, Stanojlović O. High dose of ethanol decreases total spectral power density in seizures induced by D,L-homocysteine thiolactone in adult rats. in General Physiology and Biophysics. 2009;28(SI):25-32.
https://hdl.handle.net/21.15107/rcub_vinar_2729 .

Rašić-Marković, Aleksandra, Đurić, Dragan M., Hrnčić, Dragan, Lončar-Stevanović, Helena, Vucevic, Danijela, Mladenovic, Dusan, Brkić, Predrag, Djuro, Macut, Stanojević, Ivana, Stanojlović, Olivera, "High dose of ethanol decreases total spectral power density in seizures induced by D,L-homocysteine thiolactone in adult rats" in General Physiology and Biophysics, 28, no. SI (2009):25-32,
https://hdl.handle.net/21.15107/rcub_vinar_2729 .

TY  - JOUR
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Krinulović, Katarina
AU  - Đurić, Dragan M.
AU  - Vasić, Vesna M.
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3364
AB  - In vitro inhibition of bovine erythrocytes acetylcholinesterase (AchE) by separate and simultaneous exposure to organophosphorous insecticide malathion and the transformation products, which are generally formed during the storage or natural as well as photochemical degradation pathways of malathion, was investigated. The increasing concentration of malathion, its oxidation product - malaoxon and isomerisation product - isomalathion inhibited AChE activity in a concentration-dependent manner. The half-maximum inhibitory concentrations (IC50 values): (3.2 +/- 0.1) x 10(-5) mol/l, (4.7 +/- 0.8) x 10(-7) mol/l and (6.0 +/- 0.5) x 10(-7) mol/l were obtained from the inhibition curves induced by malathion, malaoxon and isomalathion, respectively. However, the products formed due to photoinduced degradation, phosphorodithioic O,O,S-trimethyl phosphorodithioic ester (OOS(S)) and O,O-dimethyl thiophosphate did not noticeably affect the enzyme activity at all investigated concentrations, while diethyl maleate inhibited the AChE activity at concentrations GT 10 mmol/l. By simultaneous exposure of the enzyme to malaoxon and isomalathion in various concentration combinations the additive effect was achieved by low concentration of inhibitors, while the antagonistic effect was obtained at high concentration ( GT = 3 x 10-7 mol/l) of inhibitors. Inhibitory power of irradiated samples of 1 x 10(-5) mol/l malathion can be attributed to the formation of malaoxon and isomalathion, organophosphates about 100 times more toxic than their parent compound, while the presence of non-inhibiting degradation product OOS(S) did not affect the inhibitor efficiency of inhibiting malathion by-products, malaoxon and isomalathion.
T2  - General Physiology and Biophysics
T1  - Inhibition of AChE by single and simultaneous exposure to malathion and its degradation products
VL  - 26
IS  - 4
SP  - 247
EP  - 253
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3364
ER  - 

@article{
author = "Krstić, Danijela Z. and Čolović, Mirjana B. and Krinulović, Katarina and Đurić, Dragan M. and Vasić, Vesna M.",
year = "2007",
abstract = "In vitro inhibition of bovine erythrocytes acetylcholinesterase (AchE) by separate and simultaneous exposure to organophosphorous insecticide malathion and the transformation products, which are generally formed during the storage or natural as well as photochemical degradation pathways of malathion, was investigated. The increasing concentration of malathion, its oxidation product - malaoxon and isomerisation product - isomalathion inhibited AChE activity in a concentration-dependent manner. The half-maximum inhibitory concentrations (IC50 values): (3.2 +/- 0.1) x 10(-5) mol/l, (4.7 +/- 0.8) x 10(-7) mol/l and (6.0 +/- 0.5) x 10(-7) mol/l were obtained from the inhibition curves induced by malathion, malaoxon and isomalathion, respectively. However, the products formed due to photoinduced degradation, phosphorodithioic O,O,S-trimethyl phosphorodithioic ester (OOS(S)) and O,O-dimethyl thiophosphate did not noticeably affect the enzyme activity at all investigated concentrations, while diethyl maleate inhibited the AChE activity at concentrations GT 10 mmol/l. By simultaneous exposure of the enzyme to malaoxon and isomalathion in various concentration combinations the additive effect was achieved by low concentration of inhibitors, while the antagonistic effect was obtained at high concentration ( GT = 3 x 10-7 mol/l) of inhibitors. Inhibitory power of irradiated samples of 1 x 10(-5) mol/l malathion can be attributed to the formation of malaoxon and isomalathion, organophosphates about 100 times more toxic than their parent compound, while the presence of non-inhibiting degradation product OOS(S) did not affect the inhibitor efficiency of inhibiting malathion by-products, malaoxon and isomalathion.",
journal = "General Physiology and Biophysics",
title = "Inhibition of AChE by single and simultaneous exposure to malathion and its degradation products",
volume = "26",
number = "4",
pages = "247-253",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3364"
}

Krstić, D. Z., Čolović, M. B., Krinulović, K., Đurić, D. M.,& Vasić, V. M.. (2007). Inhibition of AChE by single and simultaneous exposure to malathion and its degradation products. in General Physiology and Biophysics, 26(4), 247-253.
https://hdl.handle.net/21.15107/rcub_vinar_3364

Krstić DZ, Čolović MB, Krinulović K, Đurić DM, Vasić VM. Inhibition of AChE by single and simultaneous exposure to malathion and its degradation products. in General Physiology and Biophysics. 2007;26(4):247-253.
https://hdl.handle.net/21.15107/rcub_vinar_3364 .

Krstić, Danijela Z., Čolović, Mirjana B., Krinulović, Katarina, Đurić, Dragan M., Vasić, Vesna M., "Inhibition of AChE by single and simultaneous exposure to malathion and its degradation products" in General Physiology and Biophysics, 26, no. 4 (2007):247-253,
https://hdl.handle.net/21.15107/rcub_vinar_3364 .

TY  - JOUR
AU  - Đurić, Dragan M.
AU  - Drndarevic, V
AU  - Vujic, D
PY  - 1999
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2290
AB  - A freezing device for cryopreservation of blood manonuclear cells has been developed. The device is microcontroller operated, allowing cell freezing by a fully automatic, unattended process. To ensure optimum preservation, the temperature in the cell suspension uniformly decreases from room temperature to -100 degrees C and then the samples are transferred to long-term storage. The performance of the device has been tested using both physiological solution and a sample of cell suspension. The control of temperature variation of cell suspension in the entire temperature range has been realised with an accuracy better than +/-0.1%. The viability of cells recovered from the frozen samples was 95%. The nitrogen consumption for one cycle of cryopreservation was 1.51. In addition to the fully automatic mode, the manual and semi-automatic modes are available for research purposes. The device has been designed using low cost and widely used electronic components and materials, it is compact and simple to operate.
T2  - Bio-Medical Materials and Engineering
T1  - Microprocessor-controlled freezing device for cryopreservation of cell samples
VL  - 9
IS  - 3
SP  - 171
EP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2290
ER  - 

@article{
author = "Đurić, Dragan M. and Drndarevic, V and Vujic, D",
year = "1999",
abstract = "A freezing device for cryopreservation of blood manonuclear cells has been developed. The device is microcontroller operated, allowing cell freezing by a fully automatic, unattended process. To ensure optimum preservation, the temperature in the cell suspension uniformly decreases from room temperature to -100 degrees C and then the samples are transferred to long-term storage. The performance of the device has been tested using both physiological solution and a sample of cell suspension. The control of temperature variation of cell suspension in the entire temperature range has been realised with an accuracy better than +/-0.1%. The viability of cells recovered from the frozen samples was 95%. The nitrogen consumption for one cycle of cryopreservation was 1.51. In addition to the fully automatic mode, the manual and semi-automatic modes are available for research purposes. The device has been designed using low cost and widely used electronic components and materials, it is compact and simple to operate.",
journal = "Bio-Medical Materials and Engineering",
title = "Microprocessor-controlled freezing device for cryopreservation of cell samples",
volume = "9",
number = "3",
pages = "171-178",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2290"
}

Đurić, D. M., Drndarevic, V.,& Vujic, D.. (1999). Microprocessor-controlled freezing device for cryopreservation of cell samples. in Bio-Medical Materials and Engineering, 9(3), 171-178.
https://hdl.handle.net/21.15107/rcub_vinar_2290

Đurić DM, Drndarevic V, Vujic D. Microprocessor-controlled freezing device for cryopreservation of cell samples. in Bio-Medical Materials and Engineering. 1999;9(3):171-178.
https://hdl.handle.net/21.15107/rcub_vinar_2290 .

Đurić, Dragan M., Drndarevic, V, Vujic, D, "Microprocessor-controlled freezing device for cryopreservation of cell samples" in Bio-Medical Materials and Engineering, 9, no. 3 (1999):171-178,
https://hdl.handle.net/21.15107/rcub_vinar_2290 .