TY  - JOUR
AU  - Pavlović, Marija
AU  - Šokarda Slavić, Marinela
AU  - Ristović, Marina
AU  - Stojanović, Sanja
AU  - Margetić, Aleksandra
AU  - Momčilović, Miloš
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11384
AB  - The main goal of this study was to examine the efficiency of a newly isolated fungus from quince, Aspergillus tubingensis FAT43, to produce the pectinolytic complex using agricultural and industrial waste as the substrate for solid state fermentation. Sugar beet pulp was the most effective substrate inducer of pectinolytic complex synthesis out of all the waste residues examined. For endo-pectinolytic and total pectinolytic activity, respectively, statistical optimization using Placked-Burman Design and Optimal (Custom) Design increased production by 2.22 and 2.15-fold, respectively. Liquification, clarification, and an increase in the amount of reducing sugar in fruit juices (apple, banana, apricot, orange, and quince) processed with pectinolytic complex were identified. Enzymatic pre-treatment considerably increases yield (14%–22%) and clarification (90%). After enzymatic treatment, the best liquefaction was observed in orange juice, whereas the best clarification was obtained in apricot juice. Additionally, the pectinolytic treatment of apricot juice resulted in the highest increase in reducing sugar concentration (11%) compared to all other enzymatically treated juices. Optimizing the production of a highly active pectinolytic complex and its efficient utilization in the processing of fruit juices, including the generation of an increasing amount of waste, are the significant outcomes of this research.
T2  - Letters in Applied Microbiology
T1  - Optimization of solid-state fermentation for enhanced production of pectinolytic complex by                    Aspergillus tubingensis                    FAT43 and its application in fruit juice processing
VL  - 76
IS  - 8
DO  - 10.1093/lambio/ovad083
ER  - 

@article{
author = "Pavlović, Marija and Šokarda Slavić, Marinela and Ristović, Marina and Stojanović, Sanja and Margetić, Aleksandra and Momčilović, Miloš and Vujčić, Zoran",
year = "2023",
abstract = "The main goal of this study was to examine the efficiency of a newly isolated fungus from quince, Aspergillus tubingensis FAT43, to produce the pectinolytic complex using agricultural and industrial waste as the substrate for solid state fermentation. Sugar beet pulp was the most effective substrate inducer of pectinolytic complex synthesis out of all the waste residues examined. For endo-pectinolytic and total pectinolytic activity, respectively, statistical optimization using Placked-Burman Design and Optimal (Custom) Design increased production by 2.22 and 2.15-fold, respectively. Liquification, clarification, and an increase in the amount of reducing sugar in fruit juices (apple, banana, apricot, orange, and quince) processed with pectinolytic complex were identified. Enzymatic pre-treatment considerably increases yield (14%–22%) and clarification (90%). After enzymatic treatment, the best liquefaction was observed in orange juice, whereas the best clarification was obtained in apricot juice. Additionally, the pectinolytic treatment of apricot juice resulted in the highest increase in reducing sugar concentration (11%) compared to all other enzymatically treated juices. Optimizing the production of a highly active pectinolytic complex and its efficient utilization in the processing of fruit juices, including the generation of an increasing amount of waste, are the significant outcomes of this research.",
journal = "Letters in Applied Microbiology",
title = "Optimization of solid-state fermentation for enhanced production of pectinolytic complex by                    Aspergillus tubingensis                    FAT43 and its application in fruit juice processing",
volume = "76",
number = "8",
doi = "10.1093/lambio/ovad083"
}

Pavlović, M., Šokarda Slavić, M., Ristović, M., Stojanović, S., Margetić, A., Momčilović, M.,& Vujčić, Z.. (2023). Optimization of solid-state fermentation for enhanced production of pectinolytic complex by                    Aspergillus tubingensis                    FAT43 and its application in fruit juice processing. in Letters in Applied Microbiology, 76(8).
https://doi.org/10.1093/lambio/ovad083

Pavlović M, Šokarda Slavić M, Ristović M, Stojanović S, Margetić A, Momčilović M, Vujčić Z. Optimization of solid-state fermentation for enhanced production of pectinolytic complex by                    Aspergillus tubingensis                    FAT43 and its application in fruit juice processing. in Letters in Applied Microbiology. 2023;76(8).
doi:10.1093/lambio/ovad083 .

Pavlović, Marija, Šokarda Slavić, Marinela, Ristović, Marina, Stojanović, Sanja, Margetić, Aleksandra, Momčilović, Miloš, Vujčić, Zoran, "Optimization of solid-state fermentation for enhanced production of pectinolytic complex by                    Aspergillus tubingensis                    FAT43 and its application in fruit juice processing" in Letters in Applied Microbiology, 76, no. 8 (2023),
https://doi.org/10.1093/lambio/ovad083 . .

TY  - CONF
AU  - Pavlović, Marija
AU  - Momčilović, Miloš
AU  - Živković, Sanja
AU  - Šokarda Slavić, Marinela  V.
AU  - Ristović, Marina
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13032
AB  - U ovom radu urađen je skrining sojeva Aspergillus spp. koji proizvode pektinaze fermentacijom u tečnom medijumu, a koje su efikasne u izbistravanju soka od jagode. Endo-pektinaze su detektovane difuzionim testom, a ukupna pektinazna aktivnost određena je DNS metodom. Izbistravanje soka nakon tretmana pektinazama određeno je merenjem transmitancije na 660 nm, a povećanje prinosa soka izmereno je centrifugiranjem. Dobijeni rezultati su pokazali da su ove pektinaze visokoefikasne za tretman soka od jagode u poređenju sa komercijalnim pektinaznim preparatom Lafase Fruit.
AB  - In this work, the screening of Aspergillus spp which produce pectinases by fermentation in a liquid medium was performed, as pectinases are effective in clarifying strawberry juice. Endo-pectinases were detected by the diffusion assay, and total pectinase activity was determined by the DNS method. The clarification of juice after pectinase treatment was determined by measuring the transmission at 660 nm, and an increase in juice yield was measured by centrifugation. The results obtained showed that these pectinases are very effective for the treatment of strawberry juice in comparison with the commercial pectinase preparation Lafase Fruit.
PB  - Belgrade : Serbian Chemical Society
C3  - 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings
T1  - Skrining pektinaznih proizvođača Aspergillus spp. za upotrebu u izbistravanju soka od jagode
T1  - Screening of pectinase-producing Aspergillus spp. for use in strawberry juice clarification
SP  - 55
EP  - 55
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13032
ER  - 

@conference{
author = "Pavlović, Marija and Momčilović, Miloš and Živković, Sanja and Šokarda Slavić, Marinela  V. and Ristović, Marina and Vujčić, Zoran",
year = "2023",
abstract = "U ovom radu urađen je skrining sojeva Aspergillus spp. koji proizvode pektinaze fermentacijom u tečnom medijumu, a koje su efikasne u izbistravanju soka od jagode. Endo-pektinaze su detektovane difuzionim testom, a ukupna pektinazna aktivnost određena je DNS metodom. Izbistravanje soka nakon tretmana pektinazama određeno je merenjem transmitancije na 660 nm, a povećanje prinosa soka izmereno je centrifugiranjem. Dobijeni rezultati su pokazali da su ove pektinaze visokoefikasne za tretman soka od jagode u poređenju sa komercijalnim pektinaznim preparatom Lafase Fruit., In this work, the screening of Aspergillus spp which produce pectinases by fermentation in a liquid medium was performed, as pectinases are effective in clarifying strawberry juice. Endo-pectinases were detected by the diffusion assay, and total pectinase activity was determined by the DNS method. The clarification of juice after pectinase treatment was determined by measuring the transmission at 660 nm, and an increase in juice yield was measured by centrifugation. The results obtained showed that these pectinases are very effective for the treatment of strawberry juice in comparison with the commercial pectinase preparation Lafase Fruit.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings",
title = "Skrining pektinaznih proizvođača Aspergillus spp. za upotrebu u izbistravanju soka od jagode, Screening of pectinase-producing Aspergillus spp. for use in strawberry juice clarification",
pages = "55-55",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13032"
}

Pavlović, M., Momčilović, M., Živković, S., Šokarda Slavić, Marinela  V., Ristović, M.,& Vujčić, Z.. (2023). Skrining pektinaznih proizvođača Aspergillus spp. za upotrebu u izbistravanju soka od jagode. in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings
Belgrade : Serbian Chemical Society., 55-55.
https://hdl.handle.net/21.15107/rcub_vinar_13032

Pavlović M, Momčilović M, Živković S, Šokarda Slavić, Marinela  V., Ristović M, Vujčić Z. Skrining pektinaznih proizvođača Aspergillus spp. za upotrebu u izbistravanju soka od jagode. in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings. 2023;:55-55.
https://hdl.handle.net/21.15107/rcub_vinar_13032 .

Pavlović, Marija, Momčilović, Miloš, Živković, Sanja, Šokarda Slavić, Marinela  V., Ristović, Marina, Vujčić, Zoran, "Skrining pektinaznih proizvođača Aspergillus spp. za upotrebu u izbistravanju soka od jagode" in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings (2023):55-55,
https://hdl.handle.net/21.15107/rcub_vinar_13032 .

TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Kojić, Milan
AU  - Margetić, Aleksandra
AU  - Ristović, Marina
AU  - Pavlović, Marija
AU  - Nikolić, Stefan
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11438
AB  - The combination of β‐oligosaccharides from enzymatically hydrolysed barley β‐glucan has attracted interest recently due to its positive effects on human health. This study aimed to assess the impact of the endo‐β‐1,3‐1,4‐glucanase enzyme from Bacillus subtilis 168 on improving the nutritional and bioactive properties of barley β‐glucan. A new procedure for the isolation of β‐glucan was developed, at a lower temperature (45 °C), enabling purity from starch contamination, without affecting the yield (6 g β‐glucan from 100 g of barley flour). The endo‐β‐1,3‐1,4‐glucanase is cloned into E. coli pQE_Ek enables the high production and purification (82% yield, 1.8 mg mL −1 and 440 U mg −1 ) of an enzyme identical to the natural one (25.5 kDa). The enzymatic reaction showed high efficiency of β‐glucan degradation by recombinant enzyme, giving a mixture of products (of which 3‐O‐β‐cellobiosyl‐D‐glucose and 3‐O‐β‐cellotriosyl‐D‐glucose are the most abundant), the reduction of viscosity (17%) and increase in antioxidant capacities by 15.2%, 30.9% and 44.0% assessed by ABTS, DPPH and ORAC, respectively. These results indicate the possible application of endo‐β‐1,3‐1,4‐glucanase enzyme in improving the properties of barley β‐glucan used as functional foods.
T2  - International Journal of Food Science & Technology
T1  - Improvement of nutritional and bioactive properties of barley β‐glucan‐based food products using Bacillus subtilis 168 endo‐β‐1,3‐1,4‐glucanase
DO  - 10.1111/ijfs.16647
ER  - 

@article{
author = "Šokarda Slavić, Marinela and Kojić, Milan and Margetić, Aleksandra and Ristović, Marina and Pavlović, Marija and Nikolić, Stefan and Vujčić, Zoran",
year = "2023",
abstract = "The combination of β‐oligosaccharides from enzymatically hydrolysed barley β‐glucan has attracted interest recently due to its positive effects on human health. This study aimed to assess the impact of the endo‐β‐1,3‐1,4‐glucanase enzyme from Bacillus subtilis 168 on improving the nutritional and bioactive properties of barley β‐glucan. A new procedure for the isolation of β‐glucan was developed, at a lower temperature (45 °C), enabling purity from starch contamination, without affecting the yield (6 g β‐glucan from 100 g of barley flour). The endo‐β‐1,3‐1,4‐glucanase is cloned into E. coli pQE_Ek enables the high production and purification (82% yield, 1.8 mg mL −1 and 440 U mg −1 ) of an enzyme identical to the natural one (25.5 kDa). The enzymatic reaction showed high efficiency of β‐glucan degradation by recombinant enzyme, giving a mixture of products (of which 3‐O‐β‐cellobiosyl‐D‐glucose and 3‐O‐β‐cellotriosyl‐D‐glucose are the most abundant), the reduction of viscosity (17%) and increase in antioxidant capacities by 15.2%, 30.9% and 44.0% assessed by ABTS, DPPH and ORAC, respectively. These results indicate the possible application of endo‐β‐1,3‐1,4‐glucanase enzyme in improving the properties of barley β‐glucan used as functional foods.",
journal = "International Journal of Food Science & Technology",
title = "Improvement of nutritional and bioactive properties of barley β‐glucan‐based food products using Bacillus subtilis 168 endo‐β‐1,3‐1,4‐glucanase",
doi = "10.1111/ijfs.16647"
}

Šokarda Slavić, M., Kojić, M., Margetić, A., Ristović, M., Pavlović, M., Nikolić, S.,& Vujčić, Z.. (2023). Improvement of nutritional and bioactive properties of barley β‐glucan‐based food products using Bacillus subtilis 168 endo‐β‐1,3‐1,4‐glucanase. in International Journal of Food Science & Technology.
https://doi.org/10.1111/ijfs.16647

Šokarda Slavić M, Kojić M, Margetić A, Ristović M, Pavlović M, Nikolić S, Vujčić Z. Improvement of nutritional and bioactive properties of barley β‐glucan‐based food products using Bacillus subtilis 168 endo‐β‐1,3‐1,4‐glucanase. in International Journal of Food Science & Technology. 2023;.
doi:10.1111/ijfs.16647 .

Šokarda Slavić, Marinela, Kojić, Milan, Margetić, Aleksandra, Ristović, Marina, Pavlović, Marija, Nikolić, Stefan, Vujčić, Zoran, "Improvement of nutritional and bioactive properties of barley β‐glucan‐based food products using Bacillus subtilis 168 endo‐β‐1,3‐1,4‐glucanase" in International Journal of Food Science & Technology (2023),
https://doi.org/10.1111/ijfs.16647 . .

TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Ralić, Vanja
AU  - Nastasijević, Branislav
AU  - Matijević, Milica
AU  - Vujčić, Zoran
AU  - Margetić, Aleksandra
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11988
AB  - Poly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible, and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box-Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS-PAGE followed by Methylene Blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. Encouraging, PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone.
AB  - Поли(γ-глутаминска киселина) (ПГK), коју производе бактерије рода Bacillus, је биоразградив, нетоксичан, биокомпатибилан и неимуноген негативно наелектрисани полимер. Због својих својстава нашао је разноврсну примену у прехрамбеној, козметичкој и фармацеутској индустрији. У овом раду, Bacillus ѕubtilis 17Б је изабран као најбољи ПГК продуцер међу педесетак тестираних природних изолата бактерија из овог рода и окарактерисан као глутамат независтан продуцер. Производња ПГК овим новоидентификованим сојем је оптимизована и десетоструко увећана коришћењем Box-Behnken експерименталног дизајна. Чистоћа ПГК након изоловања и пречишћавања из ферметационе течности је потврђена електрофорезом (SDS-PAGE) након бојења метиленским плавим. ПГК је окарактерисана масеном спекроскопијом (ESI MS) и коришћена за добијање новог нанокомпозита са ТiО2. Синтеза ПГК/ТiО2 нанокомпозита, његова структурна анализа и цитотоксични ефекат на ћелијску линију рака грлића материце (HeLa ћелије) је испитан да би се утврдила потенцијална употреба овог новодобијеног материјала у борби против ћелија рака. Нанокомпозит ПГК/ТiО2показао је повећан цитотоксични ефекат на поменуте ћелије рака у поређењу са самим ТiО2.
T2  - Journal of the Serbian Chemical Society
T1  - A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain
T1  - Нови ПГК/TiO2 нанокомпозит добијен од поли(γ -глутаминске киселине) из новоизолованог соја bacillus subtilis 17B
VL  - 88
IS  - 10
SP  - 985
EP  - 997
DO  - 10.2298/JSC221116011S
ER  - 

@article{
author = "Šokarda Slavić, Marinela and Ralić, Vanja and Nastasijević, Branislav and Matijević, Milica and Vujčić, Zoran and Margetić, Aleksandra",
year = "2023",
abstract = "Poly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible, and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box-Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS-PAGE followed by Methylene Blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. Encouraging, PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone., Поли(γ-глутаминска киселина) (ПГK), коју производе бактерије рода Bacillus, је биоразградив, нетоксичан, биокомпатибилан и неимуноген негативно наелектрисани полимер. Због својих својстава нашао је разноврсну примену у прехрамбеној, козметичкој и фармацеутској индустрији. У овом раду, Bacillus ѕubtilis 17Б је изабран као најбољи ПГК продуцер међу педесетак тестираних природних изолата бактерија из овог рода и окарактерисан као глутамат независтан продуцер. Производња ПГК овим новоидентификованим сојем је оптимизована и десетоструко увећана коришћењем Box-Behnken експерименталног дизајна. Чистоћа ПГК након изоловања и пречишћавања из ферметационе течности је потврђена електрофорезом (SDS-PAGE) након бојења метиленским плавим. ПГК је окарактерисана масеном спекроскопијом (ESI MS) и коришћена за добијање новог нанокомпозита са ТiО2. Синтеза ПГК/ТiО2 нанокомпозита, његова структурна анализа и цитотоксични ефекат на ћелијску линију рака грлића материце (HeLa ћелије) је испитан да би се утврдила потенцијална употреба овог новодобијеног материјала у борби против ћелија рака. Нанокомпозит ПГК/ТiО2показао је повећан цитотоксични ефекат на поменуте ћелије рака у поређењу са самим ТiО2.",
journal = "Journal of the Serbian Chemical Society",
title = "A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain, Нови ПГК/TiO2 нанокомпозит добијен од поли(γ -глутаминске киселине) из новоизолованог соја bacillus subtilis 17B",
volume = "88",
number = "10",
pages = "985-997",
doi = "10.2298/JSC221116011S"
}

Šokarda Slavić, M., Ralić, V., Nastasijević, B., Matijević, M., Vujčić, Z.,& Margetić, A.. (2023). A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain. in Journal of the Serbian Chemical Society, 88(10), 985-997.
https://doi.org/10.2298/JSC221116011S

Šokarda Slavić M, Ralić V, Nastasijević B, Matijević M, Vujčić Z, Margetić A. A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain. in Journal of the Serbian Chemical Society. 2023;88(10):985-997.
doi:10.2298/JSC221116011S .

Šokarda Slavić, Marinela, Ralić, Vanja, Nastasijević, Branislav, Matijević, Milica, Vujčić, Zoran, Margetić, Aleksandra, "A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain" in Journal of the Serbian Chemical Society, 88, no. 10 (2023):985-997,
https://doi.org/10.2298/JSC221116011S . .

TY  - JOUR
AU  - Pavlović, Ratko
AU  - Dojnov, Biljana
AU  - Šokarda Slavić, Marinela
AU  - Pavlović, Marija
AU  - Slomo, Katarina
AU  - Ristović, Marina
AU  - Vujčić, Zoran
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10303
AB  - Finding a pollen substitute for honey bees that is nutritionally adequate and affordable is a scientific and practical challenge. We attempted a new rational approach and tried to exploit honey bees’ natural cannibalistic behavior. We tested processed insect larvae as a food source that is nutritionally similar to bee brood, and which can easily be produced on a large scale. In cage experiments, monitoring bee mortality, food consumption and changes in bee body parts’ weights showed that flour obtained by grinding dried yellow mealworm larvae has the potential to become an excellent component for pollen substitution. Bees from the cage group fed Tenebrio molitor patties (TG) demonstrated overall best results in comparison to sugar patties fed bee group (CG), yeast patties fed bee group (YG) and pollen patties fed bee group (PG). They did not lose weight as rapidly as the CG, did not defecate inside cages as the YG, nor show increased mortality as the PG. At the same time, TG consumed less food (mean 13.7 g/cage) than CG (16.8 g/cage), YG (20.4 g/cage) and PG (23.9 g/cage) within the period of 28 days. Bees’ gut increase in weight was lowest in the CG, followed by TG and PG and was the highest in the YG which resulted in diarrhea after 14 days. Bees from TG did not lag behind other bees in head, thorax and abdomen weight after 28 days. We demonstrated that processed yellow mealworm larvae (T. molitor) can be used as an ingredient for honey bee feed.
T2  - Journal of Apicultural Research
T1  - In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed
SP  - 1
EP  - 10
DO  - 10.1080/00218839.2022.2080950
ER  - 

@article{
author = "Pavlović, Ratko and Dojnov, Biljana and Šokarda Slavić, Marinela and Pavlović, Marija and Slomo, Katarina and Ristović, Marina and Vujčić, Zoran",
year = "2022",
abstract = "Finding a pollen substitute for honey bees that is nutritionally adequate and affordable is a scientific and practical challenge. We attempted a new rational approach and tried to exploit honey bees’ natural cannibalistic behavior. We tested processed insect larvae as a food source that is nutritionally similar to bee brood, and which can easily be produced on a large scale. In cage experiments, monitoring bee mortality, food consumption and changes in bee body parts’ weights showed that flour obtained by grinding dried yellow mealworm larvae has the potential to become an excellent component for pollen substitution. Bees from the cage group fed Tenebrio molitor patties (TG) demonstrated overall best results in comparison to sugar patties fed bee group (CG), yeast patties fed bee group (YG) and pollen patties fed bee group (PG). They did not lose weight as rapidly as the CG, did not defecate inside cages as the YG, nor show increased mortality as the PG. At the same time, TG consumed less food (mean 13.7 g/cage) than CG (16.8 g/cage), YG (20.4 g/cage) and PG (23.9 g/cage) within the period of 28 days. Bees’ gut increase in weight was lowest in the CG, followed by TG and PG and was the highest in the YG which resulted in diarrhea after 14 days. Bees from TG did not lag behind other bees in head, thorax and abdomen weight after 28 days. We demonstrated that processed yellow mealworm larvae (T. molitor) can be used as an ingredient for honey bee feed.",
journal = "Journal of Apicultural Research",
title = "In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed",
pages = "1-10",
doi = "10.1080/00218839.2022.2080950"
}

Pavlović, R., Dojnov, B., Šokarda Slavić, M., Pavlović, M., Slomo, K., Ristović, M.,& Vujčić, Z.. (2022). In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed. in Journal of Apicultural Research, 1-10.
https://doi.org/10.1080/00218839.2022.2080950

Pavlović R, Dojnov B, Šokarda Slavić M, Pavlović M, Slomo K, Ristović M, Vujčić Z. In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed. in Journal of Apicultural Research. 2022;:1-10.
doi:10.1080/00218839.2022.2080950 .

Pavlović, Ratko, Dojnov, Biljana, Šokarda Slavić, Marinela, Pavlović, Marija, Slomo, Katarina, Ristović, Marina, Vujčić, Zoran, "In pursuit of the ultimate pollen substitute (insect larvae) for honey bee (Apis mellifera) feed" in Journal of Apicultural Research (2022):1-10,
https://doi.org/10.1080/00218839.2022.2080950 . .

TY  - CONF
AU  - Pavlović, Marija
AU  - Margetić, Aleksandra
AU  - Šokarda Slavić, Marinela
AU  - Ristović, Marina
AU  - Pavlović, Ratko
AU  - Nikolić, Stefan
AU  - Vujčić, Zoran
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11010
AB  - Pectinases are widely used in the fruit juice industry for clarification, liquefaction and stabilization of juices 1. One of the biggest problems in the production of fruit juices is the turbidity of the juice, which is mainly caused by the presence of pectin polysaccharides. Therefore, pectinase is used in juice clarification, which breaks down the pectin structure and reduces unwanted cloudiness and sediment2. In this work, the production of pectinases was optimized by solid state fermentation using Aspergillus tubingensis strain, which proved to be an efficient producer of these enzymes. Statistical method Design of Experiment was used to optimize the medium and conditions for enzyme production. The total pectinase activity obtained was determined by the DNS method (47 U/mL). Endo- pectinases activity is determined by reduction of viscosity of pectin solutions. The resulting complex of pectinase enzymes was used for the liquefaction of apricot and blueberry pulp, with a juice yield of 72% and 81%, respectively. Also, apricot juice treated with enzymes was clarified by 77% compared to juice that was not treated with enzymes. Blueberry juice obtained after treatment with pectinase enzymes has a higher antioxidant activity than the untreated juice, as determined by the DPPH assay.
PB  - Belgrade : Faculty of Chemistry : Serbian Biochemical Society
C3  - Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia
T1  - Production and application of pectinases in the liquefaction of apricot and blueberry juice
SP  - 115
EP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11010
ER  - 

@conference{
author = "Pavlović, Marija and Margetić, Aleksandra and Šokarda Slavić, Marinela and Ristović, Marina and Pavlović, Ratko and Nikolić, Stefan and Vujčić, Zoran",
year = "2022",
abstract = "Pectinases are widely used in the fruit juice industry for clarification, liquefaction and stabilization of juices 1. One of the biggest problems in the production of fruit juices is the turbidity of the juice, which is mainly caused by the presence of pectin polysaccharides. Therefore, pectinase is used in juice clarification, which breaks down the pectin structure and reduces unwanted cloudiness and sediment2. In this work, the production of pectinases was optimized by solid state fermentation using Aspergillus tubingensis strain, which proved to be an efficient producer of these enzymes. Statistical method Design of Experiment was used to optimize the medium and conditions for enzyme production. The total pectinase activity obtained was determined by the DNS method (47 U/mL). Endo- pectinases activity is determined by reduction of viscosity of pectin solutions. The resulting complex of pectinase enzymes was used for the liquefaction of apricot and blueberry pulp, with a juice yield of 72% and 81%, respectively. Also, apricot juice treated with enzymes was clarified by 77% compared to juice that was not treated with enzymes. Blueberry juice obtained after treatment with pectinase enzymes has a higher antioxidant activity than the untreated juice, as determined by the DPPH assay.",
publisher = "Belgrade : Faculty of Chemistry : Serbian Biochemical Society",
journal = "Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia",
title = "Production and application of pectinases in the liquefaction of apricot and blueberry juice",
pages = "115-116",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11010"
}

Pavlović, M., Margetić, A., Šokarda Slavić, M., Ristović, M., Pavlović, R., Nikolić, S.,& Vujčić, Z.. (2022). Production and application of pectinases in the liquefaction of apricot and blueberry juice. in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia
Belgrade : Faculty of Chemistry : Serbian Biochemical Society., 115-116.
https://hdl.handle.net/21.15107/rcub_vinar_11010

Pavlović M, Margetić A, Šokarda Slavić M, Ristović M, Pavlović R, Nikolić S, Vujčić Z. Production and application of pectinases in the liquefaction of apricot and blueberry juice. in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia. 2022;:115-116.
https://hdl.handle.net/21.15107/rcub_vinar_11010 .

Pavlović, Marija, Margetić, Aleksandra, Šokarda Slavić, Marinela, Ristović, Marina, Pavlović, Ratko, Nikolić, Stefan, Vujčić, Zoran, "Production and application of pectinases in the liquefaction of apricot and blueberry juice" in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia (2022):115-116,
https://hdl.handle.net/21.15107/rcub_vinar_11010 .

TY  - JOUR
AU  - Nišavić, Marija
AU  - Janjić, Goran V.
AU  - Hozić, Amela
AU  - Petković, Marijana
AU  - Milčić, Miloš K.
AU  - Vujčić, Zoran
AU  - Cindrić, Mario
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10180
AB  - Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.
T2  - Metallomics
T1  - Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins
VL  - 10
IS  - 4
SP  - 587
EP  - 594
DO  - 10.1039/c7mt00330g
ER  - 

@article{
author = "Nišavić, Marija and Janjić, Goran V. and Hozić, Amela and Petković, Marijana and Milčić, Miloš K. and Vujčić, Zoran and Cindrić, Mario",
year = "2018",
abstract = "Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.",
journal = "Metallomics",
title = "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins",
volume = "10",
number = "4",
pages = "587-594",
doi = "10.1039/c7mt00330g"
}

Nišavić, M., Janjić, G. V., Hozić, A., Petković, M., Milčić, M. K., Vujčić, Z.,& Cindrić, M.. (2018). Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics, 10(4), 587-594.
https://doi.org/10.1039/c7mt00330g

Nišavić M, Janjić GV, Hozić A, Petković M, Milčić MK, Vujčić Z, Cindrić M. Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics. 2018;10(4):587-594.
doi:10.1039/c7mt00330g .

Nišavić, Marija, Janjić, Goran V., Hozić, Amela, Petković, Marijana, Milčić, Miloš K., Vujčić, Zoran, Cindrić, Mario, "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins" in Metallomics, 10, no. 4 (2018):587-594,
https://doi.org/10.1039/c7mt00330g . .

TY  - JOUR
AU  - Nišavić, Marija
AU  - Janjić, Goran V.
AU  - Hozić, Amela
AU  - Petković, Marijana
AU  - Milčić, Miloš K.
AU  - Vujčić, Zoran
AU  - Cindrić, Mario
PY  - 2018
UR  - http://xlink.rsc.org/?DOI=C7MT00330G
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7615
AB  - Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.
T2  - Metallomics
T1  - Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins
VL  - 10
IS  - 4
SP  - 587
EP  - 594
DO  - 10.1039/C7MT00330G
ER  - 

@article{
author = "Nišavić, Marija and Janjić, Goran V. and Hozić, Amela and Petković, Marijana and Milčić, Miloš K. and Vujčić, Zoran and Cindrić, Mario",
year = "2018",
abstract = "Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.",
journal = "Metallomics",
title = "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins",
volume = "10",
number = "4",
pages = "587-594",
doi = "10.1039/C7MT00330G"
}

Nišavić, M., Janjić, G. V., Hozić, A., Petković, M., Milčić, M. K., Vujčić, Z.,& Cindrić, M.. (2018). Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics, 10(4), 587-594.
https://doi.org/10.1039/C7MT00330G

Nišavić M, Janjić GV, Hozić A, Petković M, Milčić MK, Vujčić Z, Cindrić M. Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics. 2018;10(4):587-594.
doi:10.1039/C7MT00330G .

Nišavić, Marija, Janjić, Goran V., Hozić, Amela, Petković, Marijana, Milčić, Miloš K., Vujčić, Zoran, Cindrić, Mario, "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins" in Metallomics, 10, no. 4 (2018):587-594,
https://doi.org/10.1039/C7MT00330G . .

TY  - JOUR
AU  - Anđelković, Uros
AU  - Theisgen, Stephan
AU  - Scheidt, Holger A.
AU  - Petković, Marijana
AU  - Huster, Daniel
AU  - Vujčić, Zoran
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4695
AB  - Understanding the effect of surface charge on the stability of proteins is one prerequisite for tailoring proteins with increased thermal stability. Here, we investigated the origin of the altered thermal stability observed between the four recently isolated isoforms (EINV1-EINV4) of external invertase. External invertase from yeast Saccharomyces cerevisiae, a homodimeric glycoprotein, represents a widely used model for studying the influence of the glyco component on protein stability. The stability of the four isoforms of invertase decreases from EINV1 to EINV4, which is accompanied by an increase in negative surface charge density. Mass spectrometry analysis revealed that the isoforms share identical protein parts indicating that the differences in stability are the result of post-translational modifications. P-31 NMR analysis revealed that the isoforms contain negatively charged phosphate groups in diester and monoester forms attached to the glycan part. The total amount of phosphate bound to the polymannan component varies between the different isoforms. These results, together with the analysis of the amount of polymannan components, show that negative surface charge density does not entirely depend on the amount of phosphate but rather on its distribution. This suggests that charged groups bound to the glyco-component of a protein can influence the stability of glycoproteins. (C) 2011 Elsevier Masson SAS. All rights reserved.
T2  - Biochimie
T1  - The thermal stability of the external invertase isoforms from Saccharomyces cerevisiae correlates with the surface charge density
VL  - 94
IS  - 2
SP  - 510
EP  - 515
DO  - 10.1016/j.biochi.2011.08.020
ER  - 

@article{
author = "Anđelković, Uros and Theisgen, Stephan and Scheidt, Holger A. and Petković, Marijana and Huster, Daniel and Vujčić, Zoran",
year = "2012",
abstract = "Understanding the effect of surface charge on the stability of proteins is one prerequisite for tailoring proteins with increased thermal stability. Here, we investigated the origin of the altered thermal stability observed between the four recently isolated isoforms (EINV1-EINV4) of external invertase. External invertase from yeast Saccharomyces cerevisiae, a homodimeric glycoprotein, represents a widely used model for studying the influence of the glyco component on protein stability. The stability of the four isoforms of invertase decreases from EINV1 to EINV4, which is accompanied by an increase in negative surface charge density. Mass spectrometry analysis revealed that the isoforms share identical protein parts indicating that the differences in stability are the result of post-translational modifications. P-31 NMR analysis revealed that the isoforms contain negatively charged phosphate groups in diester and monoester forms attached to the glycan part. The total amount of phosphate bound to the polymannan component varies between the different isoforms. These results, together with the analysis of the amount of polymannan components, show that negative surface charge density does not entirely depend on the amount of phosphate but rather on its distribution. This suggests that charged groups bound to the glyco-component of a protein can influence the stability of glycoproteins. (C) 2011 Elsevier Masson SAS. All rights reserved.",
journal = "Biochimie",
title = "The thermal stability of the external invertase isoforms from Saccharomyces cerevisiae correlates with the surface charge density",
volume = "94",
number = "2",
pages = "510-515",
doi = "10.1016/j.biochi.2011.08.020"
}

Anđelković, U., Theisgen, S., Scheidt, H. A., Petković, M., Huster, D.,& Vujčić, Z.. (2012). The thermal stability of the external invertase isoforms from Saccharomyces cerevisiae correlates with the surface charge density. in Biochimie, 94(2), 510-515.
https://doi.org/10.1016/j.biochi.2011.08.020

Anđelković U, Theisgen S, Scheidt HA, Petković M, Huster D, Vujčić Z. The thermal stability of the external invertase isoforms from Saccharomyces cerevisiae correlates with the surface charge density. in Biochimie. 2012;94(2):510-515.
doi:10.1016/j.biochi.2011.08.020 .

Anđelković, Uros, Theisgen, Stephan, Scheidt, Holger A., Petković, Marijana, Huster, Daniel, Vujčić, Zoran, "The thermal stability of the external invertase isoforms from Saccharomyces cerevisiae correlates with the surface charge density" in Biochimie, 94, no. 2 (2012):510-515,
https://doi.org/10.1016/j.biochi.2011.08.020 . .

TY  - JOUR
AU  - Momić, Tatjana
AU  - Vujčić, Zoran
AU  - Vasić, Vesna M.
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3464
AB  - The inhibition of myeloperoxidase (MPO), isolated from human neutrophils, by quercetin was investigated by following peroxidase activity of the enzyme using o-dianisidine as the substrate. The inhibition parameters (IC(50)) were obtained by graphical analysis of the inhibition curves. A reaction mechanism, which involved the enzyme inhibition by quercetin and H(2)O(2) in excess, was proposed. The rate and equilibrium constants for the proposed reaction path were calculated from experimental data. Kinetic analysis in noninhibiting H(2)O(2) concentration range in the absence and the presence of quercetin revealed that the reaction mechanism underwent Michaelis-Menten kinetics. K(m)(app,H2O2) and V(max)(app) values indicated that quercetin was a mixed inhibitor of MPO activity. The initial reaction rates were recalculated using the obtained results. Calculated curves fitted the experimental results within the range of experimental error. (C) 2008 Wiley Periodicals, Inc.
T2  - International Journal of Chemical Kinetics
T1  - Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin
VL  - 40
IS  - 7
SP  - 384
EP  - 394
DO  - 10.1002/kin.20319
ER  - 

@article{
author = "Momić, Tatjana and Vujčić, Zoran and Vasić, Vesna M.",
year = "2008",
abstract = "The inhibition of myeloperoxidase (MPO), isolated from human neutrophils, by quercetin was investigated by following peroxidase activity of the enzyme using o-dianisidine as the substrate. The inhibition parameters (IC(50)) were obtained by graphical analysis of the inhibition curves. A reaction mechanism, which involved the enzyme inhibition by quercetin and H(2)O(2) in excess, was proposed. The rate and equilibrium constants for the proposed reaction path were calculated from experimental data. Kinetic analysis in noninhibiting H(2)O(2) concentration range in the absence and the presence of quercetin revealed that the reaction mechanism underwent Michaelis-Menten kinetics. K(m)(app,H2O2) and V(max)(app) values indicated that quercetin was a mixed inhibitor of MPO activity. The initial reaction rates were recalculated using the obtained results. Calculated curves fitted the experimental results within the range of experimental error. (C) 2008 Wiley Periodicals, Inc.",
journal = "International Journal of Chemical Kinetics",
title = "Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin",
volume = "40",
number = "7",
pages = "384-394",
doi = "10.1002/kin.20319"
}

Momić, T., Vujčić, Z.,& Vasić, V. M.. (2008). Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin. in International Journal of Chemical Kinetics, 40(7), 384-394.
https://doi.org/10.1002/kin.20319

Momić T, Vujčić Z, Vasić VM. Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin. in International Journal of Chemical Kinetics. 2008;40(7):384-394.
doi:10.1002/kin.20319 .

Momić, Tatjana, Vujčić, Zoran, Vasić, Vesna M., "Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin" in International Journal of Chemical Kinetics, 40, no. 7 (2008):384-394,
https://doi.org/10.1002/kin.20319 . .

TY  - CONF
AU  - Jovanović, Ivana
AU  - Petković, Marijana
AU  - Vujčić, Zoran
AU  - Jordović, Branka
AU  - Ignjatović, Nenad L.
AU  - Uskoković, Dragan
PY  - 2007
UR  - http://dais.sanu.ac.rs/123456789/319
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7397
AB  - Poster presented at the 9th Conference of the Yugoslav Materials Research Society - YUCOMAT 2007, Herceg Novi, Crna Gora, September 10-14, 2007.
T1  - PDLLA Microparticles Containing BSA: Effect of Formulation Variables on Size Distribution
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7397
ER  - 

@conference{
author = "Jovanović, Ivana and Petković, Marijana and Vujčić, Zoran and Jordović, Branka and Ignjatović, Nenad L. and Uskoković, Dragan",
year = "2007",
abstract = "Poster presented at the 9th Conference of the Yugoslav Materials Research Society - YUCOMAT 2007, Herceg Novi, Crna Gora, September 10-14, 2007.",
title = "PDLLA Microparticles Containing BSA: Effect of Formulation Variables on Size Distribution",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7397"
}

Jovanović, I., Petković, M., Vujčić, Z., Jordović, B., Ignjatović, N. L.,& Uskoković, D.. (2007). PDLLA Microparticles Containing BSA: Effect of Formulation Variables on Size Distribution. .
https://hdl.handle.net/21.15107/rcub_vinar_7397

Jovanović I, Petković M, Vujčić Z, Jordović B, Ignjatović NL, Uskoković D. PDLLA Microparticles Containing BSA: Effect of Formulation Variables on Size Distribution. 2007;.
https://hdl.handle.net/21.15107/rcub_vinar_7397 .

Jovanović, Ivana, Petković, Marijana, Vujčić, Zoran, Jordović, Branka, Ignjatović, Nenad L., Uskoković, Dragan, "PDLLA Microparticles Containing BSA: Effect of Formulation Variables on Size Distribution" (2007),
https://hdl.handle.net/21.15107/rcub_vinar_7397 .

TY  - CONF
AU  - Momić, Tatjana
AU  - Vujčić, Zoran
AU  - Vasić, Vesna M.
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9425
AB  - Reaction mechanism of quercetin induced inhibition of myeloperoxidase isolated from human neutrophils was proposed by following peroxidase activity of the enzyme, using the o-dianisidine and H2O2 as substrates. The dependence of initial reaction rate vs. H2O2 concentration in the absence and presence of quercetin revealed the reaction mechanism that involved the enzyme inhibition by the excess of the substrate. The rate and equililbria constants for proposed reaction paths were determined
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
T1  - Inhibition of myeloperoxidase by quercetin
SP  - 359
EP  - 361
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9425
ER  - 

@conference{
author = "Momić, Tatjana and Vujčić, Zoran and Vasić, Vesna M.",
year = "2006",
abstract = "Reaction mechanism of quercetin induced inhibition of myeloperoxidase isolated from human neutrophils was proposed by following peroxidase activity of the enzyme, using the o-dianisidine and H2O2 as substrates. The dependence of initial reaction rate vs. H2O2 concentration in the absence and presence of quercetin revealed the reaction mechanism that involved the enzyme inhibition by the excess of the substrate. The rate and equililbria constants for proposed reaction paths were determined",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry",
title = "Inhibition of myeloperoxidase by quercetin",
pages = "359-361",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9425"
}

Momić, T., Vujčić, Z.,& Vasić, V. M.. (2006). Inhibition of myeloperoxidase by quercetin. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 359-361.
https://hdl.handle.net/21.15107/rcub_vinar_9425

Momić T, Vujčić Z, Vasić VM. Inhibition of myeloperoxidase by quercetin. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry. 2006;:359-361.
https://hdl.handle.net/21.15107/rcub_vinar_9425 .

Momić, Tatjana, Vujčić, Zoran, Vasić, Vesna M., "Inhibition of myeloperoxidase by quercetin" in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry (2006):359-361,
https://hdl.handle.net/21.15107/rcub_vinar_9425 .

TY  - JOUR
AU  - Momić, Tatjana
AU  - Vujčić, Zoran
AU  - Vasić, Vesna M.
AU  - Horvat, Anica
PY  - 2002
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2597
AB  - Rat brain Na,K-ATPase partially purified by SDS from synaptic plasma membranes (SPM) was immobilized by adsorption on nitrocellulose (NC), polyvinylidene fluoride (PVDF) and glass fiber (GF) membranes, Partial SDS solubilization increased the enzyme activity by 40%. With regard to the presentation of the enzyme activity. nitrocellulose Was shown to be the optimal support for die immobilization, The enzyme showed the highest percentage activity (14%) after 30 min of SPM adsorption. at 20 degreesC under the vaccum. with 25 mug of proteins per NC disc filter. In addition, adsorption on NC stabilizes the Na,K-ATPase, since the activity was substantial 72 h after adsorption at 20 degreesC, After adsorption. the sensitivity of the enzyme to HgCl2 and CdCl2 inhibition was higher, The results show that immobilized Na,K-NTPase SPM can be used as a practical model for the detection of metal ions in different samples.
T2  - Journal of the Serbian Chemical Society
T1  - Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes
VL  - 67
IS  - 12
SP  - 809
EP  - 817
DO  - 10.2298/JSC0212809M
ER  - 

@article{
author = "Momić, Tatjana and Vujčić, Zoran and Vasić, Vesna M. and Horvat, Anica",
year = "2002",
abstract = "Rat brain Na,K-ATPase partially purified by SDS from synaptic plasma membranes (SPM) was immobilized by adsorption on nitrocellulose (NC), polyvinylidene fluoride (PVDF) and glass fiber (GF) membranes, Partial SDS solubilization increased the enzyme activity by 40%. With regard to the presentation of the enzyme activity. nitrocellulose Was shown to be the optimal support for die immobilization, The enzyme showed the highest percentage activity (14%) after 30 min of SPM adsorption. at 20 degreesC under the vaccum. with 25 mug of proteins per NC disc filter. In addition, adsorption on NC stabilizes the Na,K-ATPase, since the activity was substantial 72 h after adsorption at 20 degreesC, After adsorption. the sensitivity of the enzyme to HgCl2 and CdCl2 inhibition was higher, The results show that immobilized Na,K-NTPase SPM can be used as a practical model for the detection of metal ions in different samples.",
journal = "Journal of the Serbian Chemical Society",
title = "Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes",
volume = "67",
number = "12",
pages = "809-817",
doi = "10.2298/JSC0212809M"
}

Momić, T., Vujčić, Z., Vasić, V. M.,& Horvat, A.. (2002). Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes. in Journal of the Serbian Chemical Society, 67(12), 809-817.
https://doi.org/10.2298/JSC0212809M

Momić T, Vujčić Z, Vasić VM, Horvat A. Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes. in Journal of the Serbian Chemical Society. 2002;67(12):809-817.
doi:10.2298/JSC0212809M .

Momić, Tatjana, Vujčić, Zoran, Vasić, Vesna M., Horvat, Anica, "Immobilization of Na,K-ATPase isolated from rat brain synaptic plasma membranes" in Journal of the Serbian Chemical Society, 67, no. 12 (2002):809-817,
https://doi.org/10.2298/JSC0212809M . .

TY  - JOUR
AU  - Gavrović, Marija
AU  - Trtić, Tatjana M.
AU  - Vujčić, Zoran
AU  - Petrović, Spomenka Z.
AU  - Jankov, Ratko M.
PY  - 1997
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2057
AB  - We investigated extracts of timothy grass pollen from four seasons (1989, 1990, 1991, and 1994) by protein content, SDS-PAGE, immunoblot, RAST, RAST inhibition, and crossed immunoelectrophoresis. Extract of the pollen from 1991 showed the lowest yield in quantitative assays. SDS-PAGE, crossed immunoelectrophoresis, RAST, and RAST inhibition expressed approximately comparable patterns for all extracts except that from 1991. Obviously, the quality of grass pollens, as shown for some ragweed (Ambrosia elatior) pollens depend on year of collection. Our findings are partially in agreement with some earlier examinations of the quality of timothy pollen from different pollen seasons.
T2  - Allergy
T1  - Comparison of allergenic potentials of timothy (Phleum pratense) pollens from different pollen seasons collected in the Belgrade area
VL  - 52
IS  - 2
SP  - 210
EP  - 214
DO  - 10.1111/j.1398-9995.1997.tb00977.x
ER  - 

@article{
author = "Gavrović, Marija and Trtić, Tatjana M. and Vujčić, Zoran and Petrović, Spomenka Z. and Jankov, Ratko M.",
year = "1997",
abstract = "We investigated extracts of timothy grass pollen from four seasons (1989, 1990, 1991, and 1994) by protein content, SDS-PAGE, immunoblot, RAST, RAST inhibition, and crossed immunoelectrophoresis. Extract of the pollen from 1991 showed the lowest yield in quantitative assays. SDS-PAGE, crossed immunoelectrophoresis, RAST, and RAST inhibition expressed approximately comparable patterns for all extracts except that from 1991. Obviously, the quality of grass pollens, as shown for some ragweed (Ambrosia elatior) pollens depend on year of collection. Our findings are partially in agreement with some earlier examinations of the quality of timothy pollen from different pollen seasons.",
journal = "Allergy",
title = "Comparison of allergenic potentials of timothy (Phleum pratense) pollens from different pollen seasons collected in the Belgrade area",
volume = "52",
number = "2",
pages = "210-214",
doi = "10.1111/j.1398-9995.1997.tb00977.x"
}

Gavrović, M., Trtić, T. M., Vujčić, Z., Petrović, S. Z.,& Jankov, R. M.. (1997). Comparison of allergenic potentials of timothy (Phleum pratense) pollens from different pollen seasons collected in the Belgrade area. in Allergy, 52(2), 210-214.
https://doi.org/10.1111/j.1398-9995.1997.tb00977.x

Gavrović M, Trtić TM, Vujčić Z, Petrović SZ, Jankov RM. Comparison of allergenic potentials of timothy (Phleum pratense) pollens from different pollen seasons collected in the Belgrade area. in Allergy. 1997;52(2):210-214.
doi:10.1111/j.1398-9995.1997.tb00977.x .

Gavrović, Marija, Trtić, Tatjana M., Vujčić, Zoran, Petrović, Spomenka Z., Jankov, Ratko M., "Comparison of allergenic potentials of timothy (Phleum pratense) pollens from different pollen seasons collected in the Belgrade area" in Allergy, 52, no. 2 (1997):210-214,
https://doi.org/10.1111/j.1398-9995.1997.tb00977.x . .

TY  - JOUR
AU  - Trtić, Tatjana M.
AU  - Vuksanovic, L
AU  - Paranos, S
AU  - Petrovic, S
AU  - Vujčić, Zoran
AU  - Plećaš, Ilija B.
AU  - Jankov, Ratko M.
PY  - 1996
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7219
AB  - One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb El was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mt extract is needed for coupling of ig BrCN activated paper discs. Coupling was performed in Na(2)CO3/NaHCO3 buffer pH 10. on 4 degrees C for 48h. Using this newely formed RAST, specific for Populus canadensis we have deteminated anti- Populus canadensis IgE antibodies as well. as cross reactivity between pollens of Populus canadensis and Populus deltoides.
T2  - Journal of Radioanalytical and Nuclear Chemistry
T1  - Development of RAST assay for determination of anti-Populus canadensis IgE antibodies
VL  - 206
IS  - 1
SP  - 145
EP  - 149
DO  - 10.1007/BF02040051
ER  - 

@article{
author = "Trtić, Tatjana M. and Vuksanovic, L and Paranos, S and Petrovic, S and Vujčić, Zoran and Plećaš, Ilija B. and Jankov, Ratko M.",
year = "1996",
abstract = "One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb El was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mt extract is needed for coupling of ig BrCN activated paper discs. Coupling was performed in Na(2)CO3/NaHCO3 buffer pH 10. on 4 degrees C for 48h. Using this newely formed RAST, specific for Populus canadensis we have deteminated anti- Populus canadensis IgE antibodies as well. as cross reactivity between pollens of Populus canadensis and Populus deltoides.",
journal = "Journal of Radioanalytical and Nuclear Chemistry",
title = "Development of RAST assay for determination of anti-Populus canadensis IgE antibodies",
volume = "206",
number = "1",
pages = "145-149",
doi = "10.1007/BF02040051"
}

Trtić, T. M., Vuksanovic, L., Paranos, S., Petrovic, S., Vujčić, Z., Plećaš, I. B.,& Jankov, R. M.. (1996). Development of RAST assay for determination of anti-Populus canadensis IgE antibodies. in Journal of Radioanalytical and Nuclear Chemistry, 206(1), 145-149.
https://doi.org/10.1007/BF02040051

Trtić TM, Vuksanovic L, Paranos S, Petrovic S, Vujčić Z, Plećaš IB, Jankov RM. Development of RAST assay for determination of anti-Populus canadensis IgE antibodies. in Journal of Radioanalytical and Nuclear Chemistry. 1996;206(1):145-149.
doi:10.1007/BF02040051 .

Trtić, Tatjana M., Vuksanovic, L, Paranos, S, Petrovic, S, Vujčić, Zoran, Plećaš, Ilija B., Jankov, Ratko M., "Development of RAST assay for determination of anti-Populus canadensis IgE antibodies" in Journal of Radioanalytical and Nuclear Chemistry, 206, no. 1 (1996):145-149,
https://doi.org/10.1007/BF02040051 . .