TY  - JOUR
AU  - Adžić, Miroslav
AU  - Lukić, Iva
AU  - Mitić, Miloš
AU  - Đorđević, Jelena D.
AU  - Elaković, Ivana
AU  - Đorđević, Ana D.
AU  - Krstić-Demonacos, Marija
AU  - Matić, Gordana
AU  - Radojčić, Marija
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5868
AB  - Antidepressants affect glucocorticoid receptor (GR) functioning partly through modulation of its phosphorylation but their effects on mitochondrial GR have remained undefined. We investigated the ability of chronic fiuoxetine treatment to affect chronic stress-induced changes of mitochondrial GR and its phosphoisoforms (pGRs) in the prefrontal cortex and hippocampus of female and male rats. Since mitochondrial GR regulates oxidative phosphorylation, expression of mitochondrial-encoded subunits of cytochrome (cyt) c oxidase and its activity were also investigated. Chronic stress caused accumulation of the GR in mitochondria of female prefrontal cortex, while the changes in the hippocampus were sex-specific at the levels of pGRs. Expression of mitochondrial COXs genes corresponded to chronic stress-modulated mitochondrial GR in both tissues of both genders and to cyt c oxidase activity in females. Moreover, the metabolic parameters in stressed animals were affected by fiuoxetine therapy only in the hippocampus. Namely, fluoxetine effects on mitochondrial COXs and cyt c oxidase activity in the hippocampus seem to be conveyed through pGR232 in females, while in males this likely occurs through other mechanisms. In summary, sex-specific regulation of cyt c oxidase by the stress and antidepressant treatment and its differential convergence with mitochondrial GR signaling in the prefrontal cortex and hippocampus could contribute to clarification of sex-dependent vulnerability to stress-related disorders and sex-specific clinical impact of antidepressants. (C) 2013 Elsevier Ltd. All rights reserved.
T2  - Psychoneuroendocrinology
T1  - Brain region- and sex-specific modulation of mitochondrial glucocorticoid receptor phosphorylation in fluoxetine treated stressed rats: Effects on energy metabolism
VL  - 38
IS  - 12
SP  - 2914
EP  - 2924
DO  - 10.1016/j.psyneuen.2013.07.019
ER  - 

@article{
author = "Adžić, Miroslav and Lukić, Iva and Mitić, Miloš and Đorđević, Jelena D. and Elaković, Ivana and Đorđević, Ana D. and Krstić-Demonacos, Marija and Matić, Gordana and Radojčić, Marija",
year = "2013",
abstract = "Antidepressants affect glucocorticoid receptor (GR) functioning partly through modulation of its phosphorylation but their effects on mitochondrial GR have remained undefined. We investigated the ability of chronic fiuoxetine treatment to affect chronic stress-induced changes of mitochondrial GR and its phosphoisoforms (pGRs) in the prefrontal cortex and hippocampus of female and male rats. Since mitochondrial GR regulates oxidative phosphorylation, expression of mitochondrial-encoded subunits of cytochrome (cyt) c oxidase and its activity were also investigated. Chronic stress caused accumulation of the GR in mitochondria of female prefrontal cortex, while the changes in the hippocampus were sex-specific at the levels of pGRs. Expression of mitochondrial COXs genes corresponded to chronic stress-modulated mitochondrial GR in both tissues of both genders and to cyt c oxidase activity in females. Moreover, the metabolic parameters in stressed animals were affected by fiuoxetine therapy only in the hippocampus. Namely, fluoxetine effects on mitochondrial COXs and cyt c oxidase activity in the hippocampus seem to be conveyed through pGR232 in females, while in males this likely occurs through other mechanisms. In summary, sex-specific regulation of cyt c oxidase by the stress and antidepressant treatment and its differential convergence with mitochondrial GR signaling in the prefrontal cortex and hippocampus could contribute to clarification of sex-dependent vulnerability to stress-related disorders and sex-specific clinical impact of antidepressants. (C) 2013 Elsevier Ltd. All rights reserved.",
journal = "Psychoneuroendocrinology",
title = "Brain region- and sex-specific modulation of mitochondrial glucocorticoid receptor phosphorylation in fluoxetine treated stressed rats: Effects on energy metabolism",
volume = "38",
number = "12",
pages = "2914-2924",
doi = "10.1016/j.psyneuen.2013.07.019"
}

Adžić, M., Lukić, I., Mitić, M., Đorđević, J. D., Elaković, I., Đorđević, A. D., Krstić-Demonacos, M., Matić, G.,& Radojčić, M.. (2013). Brain region- and sex-specific modulation of mitochondrial glucocorticoid receptor phosphorylation in fluoxetine treated stressed rats: Effects on energy metabolism. in Psychoneuroendocrinology, 38(12), 2914-2924.
https://doi.org/10.1016/j.psyneuen.2013.07.019

Adžić M, Lukić I, Mitić M, Đorđević JD, Elaković I, Đorđević AD, Krstić-Demonacos M, Matić G, Radojčić M. Brain region- and sex-specific modulation of mitochondrial glucocorticoid receptor phosphorylation in fluoxetine treated stressed rats: Effects on energy metabolism. in Psychoneuroendocrinology. 2013;38(12):2914-2924.
doi:10.1016/j.psyneuen.2013.07.019 .

Adžić, Miroslav, Lukić, Iva, Mitić, Miloš, Đorđević, Jelena D., Elaković, Ivana, Đorđević, Ana D., Krstić-Demonacos, Marija, Matić, Gordana, Radojčić, Marija, "Brain region- and sex-specific modulation of mitochondrial glucocorticoid receptor phosphorylation in fluoxetine treated stressed rats: Effects on energy metabolism" in Psychoneuroendocrinology, 38, no. 12 (2013):2914-2924,
https://doi.org/10.1016/j.psyneuen.2013.07.019 . .

TY  - JOUR
AU  - Simić, Iva
AU  - Adžić, Miroslav
AU  - Marić, Nađa
AU  - Savić, Danka A.
AU  - Đorđević, Jelena D.
AU  - Mihaljević, Marina
AU  - Mitić, Miloš
AU  - Pavlović, Zorana
AU  - Soldatovic, Ivan
AU  - Krstić-Demonacos, Marija
AU  - Jasovic-Gasic, Miroslava
AU  - Radoičić, Marija B.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5753
AB  - The mechanism of maladaptive chronic stress response involves altered phosphorylation of the glucocorticoid receptor (GR). In this study, we investigated if important depressogenic vulnerability factors, such as neuroticism and self-reports of negative affective states, may be associated with alterations in levels of the GR and GR phosphoisoforms in peripheral blood mononuclear cells (PBMC) of healthy adults. In 21 women and 16 men we evaluated PMBC levels of total GR (tGR), GR phosphorylated at serine 211 (pGR-S211) and serine 226 (pGR-S226) and correlated these data with personality traits and current reports of stress, anxiety and depression. Also, we assessed plasma cortisol levels in all tested subjects. Our results showed that in women nuclear pGR-S226 was positively correlated with neuroticism and current reports of depression, anxiety and stress, while the ratio of nuclear pGR-S211/pGR-S226 was negatively correlated with reports of depression. None of the aforementioned correlations were significant in men. No significant relations between cortisol levels and any of GR parameters were observed. These preliminary findings highlight the value of GR phosphorylation-related research in identifying molecular biomarkers of depressogenic vulnerability, at least in women. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
T2  - Psychiatry Research
T1  - A preliminary evaluation of leukocyte phospho-glucocorticoid receptor as a potential biomarker of depressogenic vulnerability in healthy adults
VL  - 209
IS  - 3
SP  - 658
EP  - 664
DO  - 10.1016/j.psychres.2013.02.002
ER  - 

@article{
author = "Simić, Iva and Adžić, Miroslav and Marić, Nađa and Savić, Danka A. and Đorđević, Jelena D. and Mihaljević, Marina and Mitić, Miloš and Pavlović, Zorana and Soldatovic, Ivan and Krstić-Demonacos, Marija and Jasovic-Gasic, Miroslava and Radoičić, Marija B.",
year = "2013",
abstract = "The mechanism of maladaptive chronic stress response involves altered phosphorylation of the glucocorticoid receptor (GR). In this study, we investigated if important depressogenic vulnerability factors, such as neuroticism and self-reports of negative affective states, may be associated with alterations in levels of the GR and GR phosphoisoforms in peripheral blood mononuclear cells (PBMC) of healthy adults. In 21 women and 16 men we evaluated PMBC levels of total GR (tGR), GR phosphorylated at serine 211 (pGR-S211) and serine 226 (pGR-S226) and correlated these data with personality traits and current reports of stress, anxiety and depression. Also, we assessed plasma cortisol levels in all tested subjects. Our results showed that in women nuclear pGR-S226 was positively correlated with neuroticism and current reports of depression, anxiety and stress, while the ratio of nuclear pGR-S211/pGR-S226 was negatively correlated with reports of depression. None of the aforementioned correlations were significant in men. No significant relations between cortisol levels and any of GR parameters were observed. These preliminary findings highlight the value of GR phosphorylation-related research in identifying molecular biomarkers of depressogenic vulnerability, at least in women. (C) 2013 Elsevier Ireland Ltd. All rights reserved.",
journal = "Psychiatry Research",
title = "A preliminary evaluation of leukocyte phospho-glucocorticoid receptor as a potential biomarker of depressogenic vulnerability in healthy adults",
volume = "209",
number = "3",
pages = "658-664",
doi = "10.1016/j.psychres.2013.02.002"
}

Simić, I., Adžić, M., Marić, N., Savić, D. A., Đorđević, J. D., Mihaljević, M., Mitić, M., Pavlović, Z., Soldatovic, I., Krstić-Demonacos, M., Jasovic-Gasic, M.,& Radoičić, M. B.. (2013). A preliminary evaluation of leukocyte phospho-glucocorticoid receptor as a potential biomarker of depressogenic vulnerability in healthy adults. in Psychiatry Research, 209(3), 658-664.
https://doi.org/10.1016/j.psychres.2013.02.002

Simić I, Adžić M, Marić N, Savić DA, Đorđević JD, Mihaljević M, Mitić M, Pavlović Z, Soldatovic I, Krstić-Demonacos M, Jasovic-Gasic M, Radoičić MB. A preliminary evaluation of leukocyte phospho-glucocorticoid receptor as a potential biomarker of depressogenic vulnerability in healthy adults. in Psychiatry Research. 2013;209(3):658-664.
doi:10.1016/j.psychres.2013.02.002 .

Simić, Iva, Adžić, Miroslav, Marić, Nađa, Savić, Danka A., Đorđević, Jelena D., Mihaljević, Marina, Mitić, Miloš, Pavlović, Zorana, Soldatovic, Ivan, Krstić-Demonacos, Marija, Jasovic-Gasic, Miroslava, Radoičić, Marija B., "A preliminary evaluation of leukocyte phospho-glucocorticoid receptor as a potential biomarker of depressogenic vulnerability in healthy adults" in Psychiatry Research, 209, no. 3 (2013):658-664,
https://doi.org/10.1016/j.psychres.2013.02.002 . .

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Ruždijić, Sabera
AU  - Kanazir, Dušan T.
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Paraskevopoulou, Elissavet
AU  - Pantelidou, Constantia
AU  - Radojčić, Marija
AU  - Demonacos, Constantinos
AU  - Krstić-Demonacos, Marija
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3999
AB  - The glucocorticoid receptor (GR) signal transduction and transcriptional regulation are efficiently recapitulated when GR is expressed in Saccharomyces cerevisiae. In this report we demonstrate that the in vivo GR phosphorylation pattern, hormone dependency and interdependency of phosphorylation events were similar in yeast and mammalian cells. GR phosphorylation at S246 exhibited inhibitory effect on S224 and S232 phosphorylation, suggesting the conservation of molecular mechanisms that control this interdependence between yeast and mammalian cells. To assess the effects of GR phosphorylation the mutated GR derivatives T171A, S224A, S232A, S246A were overexpressed and their transcriptional activity was analysed. These receptor derivatives displayed significant hormone inducible transcription when overexpressed in S. cerevisiae. We have established an inducible methionine expression system, which allows the close regulation of the receptor protein levels to analyse the dependence of GR function on its phosphorylation and protein abundance. Using this system we observed that GR S246A mutation increased its activity across all of the GR concentrations tested. The activity of the S224A and S246A mutants was mostly independent of GR protein levels, whereas the WT, T171A and S232A mediated transcription diminished with declining GR protein levels. Our results suggest that GR phosphorylation at specific residues affects its transcriptional functions in a site selective manner and these effects were directly linked to GR dosage. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
T2  - Steroids
T1  - Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae
VL  - 75
IS  - 6
SP  - 457
EP  - 465
DO  - 10.1016/j.steroids.2010.03.001
ER  - 

@article{
author = "Popović, Nataša M. and Ruždijić, Sabera and Kanazir, Dušan T. and Nićiforović, Ana and Adžić, Miroslav and Paraskevopoulou, Elissavet and Pantelidou, Constantia and Radojčić, Marija and Demonacos, Constantinos and Krstić-Demonacos, Marija",
year = "2010",
abstract = "The glucocorticoid receptor (GR) signal transduction and transcriptional regulation are efficiently recapitulated when GR is expressed in Saccharomyces cerevisiae. In this report we demonstrate that the in vivo GR phosphorylation pattern, hormone dependency and interdependency of phosphorylation events were similar in yeast and mammalian cells. GR phosphorylation at S246 exhibited inhibitory effect on S224 and S232 phosphorylation, suggesting the conservation of molecular mechanisms that control this interdependence between yeast and mammalian cells. To assess the effects of GR phosphorylation the mutated GR derivatives T171A, S224A, S232A, S246A were overexpressed and their transcriptional activity was analysed. These receptor derivatives displayed significant hormone inducible transcription when overexpressed in S. cerevisiae. We have established an inducible methionine expression system, which allows the close regulation of the receptor protein levels to analyse the dependence of GR function on its phosphorylation and protein abundance. Using this system we observed that GR S246A mutation increased its activity across all of the GR concentrations tested. The activity of the S224A and S246A mutants was mostly independent of GR protein levels, whereas the WT, T171A and S232A mediated transcription diminished with declining GR protein levels. Our results suggest that GR phosphorylation at specific residues affects its transcriptional functions in a site selective manner and these effects were directly linked to GR dosage. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.",
journal = "Steroids",
title = "Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae",
volume = "75",
number = "6",
pages = "457-465",
doi = "10.1016/j.steroids.2010.03.001"
}

Popović, N. M., Ruždijić, S., Kanazir, D. T., Nićiforović, A., Adžić, M., Paraskevopoulou, E., Pantelidou, C., Radojčić, M., Demonacos, C.,& Krstić-Demonacos, M.. (2010). Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae. in Steroids, 75(6), 457-465.
https://doi.org/10.1016/j.steroids.2010.03.001

Popović NM, Ruždijić S, Kanazir DT, Nićiforović A, Adžić M, Paraskevopoulou E, Pantelidou C, Radojčić M, Demonacos C, Krstić-Demonacos M. Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae. in Steroids. 2010;75(6):457-465.
doi:10.1016/j.steroids.2010.03.001 .

Popović, Nataša M., Ruždijić, Sabera, Kanazir, Dušan T., Nićiforović, Ana, Adžić, Miroslav, Paraskevopoulou, Elissavet, Pantelidou, Constantia, Radojčić, Marija, Demonacos, Constantinos, Krstić-Demonacos, Marija, "Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae" in Steroids, 75, no. 6 (2010):457-465,
https://doi.org/10.1016/j.steroids.2010.03.001 . .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Đorđević, Jelena D.
AU  - Đorđević, Ana D.
AU  - Nićiforović, Ana
AU  - Demonacos, Constantinos
AU  - Radoičić, Marija B.
AU  - Krstić-Demonacos, Marija
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3848
AB  - Chronic stress and impaired glucocorticoid receptor (GR) feedback are important factors for the compromised hypothalamic-pituitary-adrenal (HPA) axis activity. We investigated the effects of chronic 2 1 day isolation of Wistar rats on the extrinsic negative feedback part of I-IPA axis: hippocampus (HIPPO) and prefrontal cortex (PFC). In addition to serum corticosterone (CORT), we followed GR subcellular localization, GR phosphorylation at serine 232 and serine 246, expression of GR, regulated genes: GR, CRF and brain-derived neurotropic factor (BDNF), and activity of c-Jun N-terminal kinase (JNK) and Cdk5 kinases that phosphorylate GR. These parameters were also determined in animals subjected to acute 30 min immobilization, which was taken as normal adaptive response to stress. In isolated animals, we found decreased CORT, whereas, in animals exposed to acute immobilization, CORT was markedly increased. Even though the GR was predominantly localized in the nucleus of HIPPO and PFC in acute, but not in chronic stress, the expression of GR, CRF, and BDNF genes was similarly regulated under both acute and chronic stresses. Thus, the transcriptional activity of GR under chronic isolation did not seem to be exclusively dependent on high serum CORT levels nor on the subcellular location of the GR protein. Rather, it resulted front the increased Cdk5 activation and phosphorylation of the nuclear GR at serine 232 and the decreased JNK activity reflected in decreased phosphorylation of the nuclear GR at serine 246. Our study suggests that this nuclear isoform of hippocampal and cortical GR may be related to hypocorticism i.e. HPA axis hypoactivity under chronic isolation stress. journal of Endocrinology (2009) 202, 87-97
T2  - Journal of Endocrinology
T1  - Acute or chronic stress induce cell compartment-specific phosphorylation of glucocorticoid receptor and alter its transcriptional activity in Wistar rat brain
VL  - 202
IS  - 1
SP  - 87
EP  - 97
DO  - 10.1677/JOE-08-0509
ER  - 

@article{
author = "Adžić, Miroslav and Đorđević, Jelena D. and Đorđević, Ana D. and Nićiforović, Ana and Demonacos, Constantinos and Radoičić, Marija B. and Krstić-Demonacos, Marija",
year = "2009",
abstract = "Chronic stress and impaired glucocorticoid receptor (GR) feedback are important factors for the compromised hypothalamic-pituitary-adrenal (HPA) axis activity. We investigated the effects of chronic 2 1 day isolation of Wistar rats on the extrinsic negative feedback part of I-IPA axis: hippocampus (HIPPO) and prefrontal cortex (PFC). In addition to serum corticosterone (CORT), we followed GR subcellular localization, GR phosphorylation at serine 232 and serine 246, expression of GR, regulated genes: GR, CRF and brain-derived neurotropic factor (BDNF), and activity of c-Jun N-terminal kinase (JNK) and Cdk5 kinases that phosphorylate GR. These parameters were also determined in animals subjected to acute 30 min immobilization, which was taken as normal adaptive response to stress. In isolated animals, we found decreased CORT, whereas, in animals exposed to acute immobilization, CORT was markedly increased. Even though the GR was predominantly localized in the nucleus of HIPPO and PFC in acute, but not in chronic stress, the expression of GR, CRF, and BDNF genes was similarly regulated under both acute and chronic stresses. Thus, the transcriptional activity of GR under chronic isolation did not seem to be exclusively dependent on high serum CORT levels nor on the subcellular location of the GR protein. Rather, it resulted front the increased Cdk5 activation and phosphorylation of the nuclear GR at serine 232 and the decreased JNK activity reflected in decreased phosphorylation of the nuclear GR at serine 246. Our study suggests that this nuclear isoform of hippocampal and cortical GR may be related to hypocorticism i.e. HPA axis hypoactivity under chronic isolation stress. journal of Endocrinology (2009) 202, 87-97",
journal = "Journal of Endocrinology",
title = "Acute or chronic stress induce cell compartment-specific phosphorylation of glucocorticoid receptor and alter its transcriptional activity in Wistar rat brain",
volume = "202",
number = "1",
pages = "87-97",
doi = "10.1677/JOE-08-0509"
}

Adžić, M., Đorđević, J. D., Đorđević, A. D., Nićiforović, A., Demonacos, C., Radoičić, M. B.,& Krstić-Demonacos, M.. (2009). Acute or chronic stress induce cell compartment-specific phosphorylation of glucocorticoid receptor and alter its transcriptional activity in Wistar rat brain. in Journal of Endocrinology, 202(1), 87-97.
https://doi.org/10.1677/JOE-08-0509

Adžić M, Đorđević JD, Đorđević AD, Nićiforović A, Demonacos C, Radoičić MB, Krstić-Demonacos M. Acute or chronic stress induce cell compartment-specific phosphorylation of glucocorticoid receptor and alter its transcriptional activity in Wistar rat brain. in Journal of Endocrinology. 2009;202(1):87-97.
doi:10.1677/JOE-08-0509 .

Adžić, Miroslav, Đorđević, Jelena D., Đorđević, Ana D., Nićiforović, Ana, Demonacos, Constantinos, Radoičić, Marija B., Krstić-Demonacos, Marija, "Acute or chronic stress induce cell compartment-specific phosphorylation of glucocorticoid receptor and alter its transcriptional activity in Wistar rat brain" in Journal of Endocrinology, 202, no. 1 (2009):87-97,
https://doi.org/10.1677/JOE-08-0509 . .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Đorđević, Ana D.
AU  - Demonacos, Constantinos
AU  - Krstić-Demonacos, Marija
AU  - Radojčić, Marija
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3810
AB  - Mitochondrial dysfunction is increasingly recognized as a key component in compromised neuroendocrine stress response and. among other etiological causes. it may also involve action of glucocorticoid hormones. In the current study we followed glucocorticoid receptor and Identified its mitochondrial phosphoisophorms in hippocampus and prefrontal brain cortex of Wistar male rats subjected to acute. chronic and combined neuroendocrine stresses. In both brain structures chronic social isolation caused m,irked increase in mitochondrial glucocorticoid receptor that was preferentially phosphorylated at serine 232 compared to serine 246 or serine 171. This increase corresponded with the decreased expression of mitochondrially encoded cytochrome oxidase subunits 1 and 3 in hippocampus, and with their increased expression in prefrontal brain cortex. Prefrontal brain cortex appeared to be more sensitive to chronic stress, since it exibited higher levels of mitochondrial Bax and cytoplasmic Bcl2 compared to hippocampus. Chronic stress also altered the response of both brain structures to subsequent acute stress according to the studied parameters. Therefore, prolonged social isolation may cause susceptibility to mitochondria triggered proapototic signalling. which at least in part may be mediated by the glucocorticoid receptor dependent mechanism. (C) 2009 Elsevier Ltd All rights reserved.
T2  - International Journal of Biochemistry and Cell Biology
T1  - The role of phosphorylated glucocorticoid receptor in mitochondrial functions and apoptotic signalling in brain tissue of stressed Wistar rats
VL  - 41
IS  - 11
SP  - 2181
EP  - 2188
DO  - 10.1016/j.biocel.2009.04.001
ER  - 

@article{
author = "Adžić, Miroslav and Đorđević, Ana D. and Demonacos, Constantinos and Krstić-Demonacos, Marija and Radojčić, Marija",
year = "2009",
abstract = "Mitochondrial dysfunction is increasingly recognized as a key component in compromised neuroendocrine stress response and. among other etiological causes. it may also involve action of glucocorticoid hormones. In the current study we followed glucocorticoid receptor and Identified its mitochondrial phosphoisophorms in hippocampus and prefrontal brain cortex of Wistar male rats subjected to acute. chronic and combined neuroendocrine stresses. In both brain structures chronic social isolation caused m,irked increase in mitochondrial glucocorticoid receptor that was preferentially phosphorylated at serine 232 compared to serine 246 or serine 171. This increase corresponded with the decreased expression of mitochondrially encoded cytochrome oxidase subunits 1 and 3 in hippocampus, and with their increased expression in prefrontal brain cortex. Prefrontal brain cortex appeared to be more sensitive to chronic stress, since it exibited higher levels of mitochondrial Bax and cytoplasmic Bcl2 compared to hippocampus. Chronic stress also altered the response of both brain structures to subsequent acute stress according to the studied parameters. Therefore, prolonged social isolation may cause susceptibility to mitochondria triggered proapototic signalling. which at least in part may be mediated by the glucocorticoid receptor dependent mechanism. (C) 2009 Elsevier Ltd All rights reserved.",
journal = "International Journal of Biochemistry and Cell Biology",
title = "The role of phosphorylated glucocorticoid receptor in mitochondrial functions and apoptotic signalling in brain tissue of stressed Wistar rats",
volume = "41",
number = "11",
pages = "2181-2188",
doi = "10.1016/j.biocel.2009.04.001"
}

Adžić, M., Đorđević, A. D., Demonacos, C., Krstić-Demonacos, M.,& Radojčić, M.. (2009). The role of phosphorylated glucocorticoid receptor in mitochondrial functions and apoptotic signalling in brain tissue of stressed Wistar rats. in International Journal of Biochemistry and Cell Biology, 41(11), 2181-2188.
https://doi.org/10.1016/j.biocel.2009.04.001

Adžić M, Đorđević AD, Demonacos C, Krstić-Demonacos M, Radojčić M. The role of phosphorylated glucocorticoid receptor in mitochondrial functions and apoptotic signalling in brain tissue of stressed Wistar rats. in International Journal of Biochemistry and Cell Biology. 2009;41(11):2181-2188.
doi:10.1016/j.biocel.2009.04.001 .

Adžić, Miroslav, Đorđević, Ana D., Demonacos, Constantinos, Krstić-Demonacos, Marija, Radojčić, Marija, "The role of phosphorylated glucocorticoid receptor in mitochondrial functions and apoptotic signalling in brain tissue of stressed Wistar rats" in International Journal of Biochemistry and Cell Biology, 41, no. 11 (2009):2181-2188,
https://doi.org/10.1016/j.biocel.2009.04.001 . .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Đorđević, Ana D.
AU  - Krstić-Demonacos, Marija
AU  - Radoičić, Marija B.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3785
AB  - In the paper entitled: Adžić, M., Đorđević, A., Krstić-Demonacos, M., & Radojčić, M. B. (2009). Stress-induced phosphorylation of c-Jun-N-terminal kinases and nuclear translocation of Hsp70 in the Wistar rat hippocampus. Archives of Biological Sciences, 61(1), 1-8.

Fig. 1, on page 4, section b, should read "Nucleus" instead of "Cytoplasm"
T2  - Archives of Biological Sciences
T1  - Errata Corrige "Stress-Induced phosphorylation of C-JUN-N-Terminal kinases and nuclear translocation of HSP70 in the wistar rat hippocampus" [Arch. Biol. Sci. Belgrade 61(1) (2009) 1-8, DOI: 10.2298/ABS0901001A]
VL  - 61
IS  - 3
SP  - 571
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3785
ER  - 

@article{
author = "Adžić, Miroslav and Đorđević, Ana D. and Krstić-Demonacos, Marija and Radoičić, Marija B.",
year = "2009",
abstract = "In the paper entitled: Adžić, M., Đorđević, A., Krstić-Demonacos, M., & Radojčić, M. B. (2009). Stress-induced phosphorylation of c-Jun-N-terminal kinases and nuclear translocation of Hsp70 in the Wistar rat hippocampus. Archives of Biological Sciences, 61(1), 1-8.

Fig. 1, on page 4, section b, should read "Nucleus" instead of "Cytoplasm"",
journal = "Archives of Biological Sciences",
title = "Errata Corrige "Stress-Induced phosphorylation of C-JUN-N-Terminal kinases and nuclear translocation of HSP70 in the wistar rat hippocampus" [Arch. Biol. Sci. Belgrade 61(1) (2009) 1-8, DOI: 10.2298/ABS0901001A]",
volume = "61",
number = "3",
pages = "571",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3785"
}

Adžić, M., Đorđević, A. D., Krstić-Demonacos, M.,& Radoičić, M. B.. (2009). Errata Corrige "Stress-Induced phosphorylation of C-JUN-N-Terminal kinases and nuclear translocation of HSP70 in the wistar rat hippocampus" [Arch. Biol. Sci. Belgrade 61(1) (2009) 1-8, DOI: 10.2298/ABS0901001A]. in Archives of Biological Sciences, 61(3), 571.
https://hdl.handle.net/21.15107/rcub_vinar_3785

Adžić M, Đorđević AD, Krstić-Demonacos M, Radoičić MB. Errata Corrige "Stress-Induced phosphorylation of C-JUN-N-Terminal kinases and nuclear translocation of HSP70 in the wistar rat hippocampus" [Arch. Biol. Sci. Belgrade 61(1) (2009) 1-8, DOI: 10.2298/ABS0901001A]. in Archives of Biological Sciences. 2009;61(3):571.
https://hdl.handle.net/21.15107/rcub_vinar_3785 .

Adžić, Miroslav, Đorđević, Ana D., Krstić-Demonacos, Marija, Radoičić, Marija B., "Errata Corrige "Stress-Induced phosphorylation of C-JUN-N-Terminal kinases and nuclear translocation of HSP70 in the wistar rat hippocampus" [Arch. Biol. Sci. Belgrade 61(1) (2009) 1-8, DOI: 10.2298/ABS0901001A]" in Archives of Biological Sciences, 61, no. 3 (2009):571,
https://hdl.handle.net/21.15107/rcub_vinar_3785 .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Đorđević, Ana D.
AU  - Krstić-Demonacos, Marija
AU  - Radojčić, Marija
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3688
AB  - Glucocorticoids are key regulators of the neuroendocrine stress response in the hippocampus. Their action is partly mediated through the subfamily of MAPKs termed c-jun-N-terminal kinases (JNKs), whose activation correlates with neurodegeneration. The stress response also involves activation of cell protective mechanisms through various heat shock proteins (HSPs) that mediate neuroprotection. We followed both JNKs and Hsp70 signals in the cytoplasmic and nuclear compartments of the hippocampus of Wistar male rats exposed to acute, chronic, and combined stress. The activity of JNK1 was decreased in both compartments by all three types of stress, while the activity of cytoplasmic JNK2/3 was elevated in acute and unaltered or lowered in chronic and combined stress. Under all stress conditions, Hsp70 translocation to the nucleus was markedly increased. The results suggest that neurodegenerative signaling of JNKs may be counteracted by increase of nuclear Hsp70, especially under chronic stress.
T2  - Archives of Biological Sciences
T1  - Stress-Induced Phosphorylation of C-JUN-N-Terminal Kinases and Nuclear Translocation of Hsp70 in the Wistar Rat Hippocampus
VL  - 61
IS  - 1
SP  - 1
EP  - 8
DO  - 10.2298/ABS0901001A
ER  - 

@article{
author = "Adžić, Miroslav and Đorđević, Ana D. and Krstić-Demonacos, Marija and Radojčić, Marija",
year = "2009",
abstract = "Glucocorticoids are key regulators of the neuroendocrine stress response in the hippocampus. Their action is partly mediated through the subfamily of MAPKs termed c-jun-N-terminal kinases (JNKs), whose activation correlates with neurodegeneration. The stress response also involves activation of cell protective mechanisms through various heat shock proteins (HSPs) that mediate neuroprotection. We followed both JNKs and Hsp70 signals in the cytoplasmic and nuclear compartments of the hippocampus of Wistar male rats exposed to acute, chronic, and combined stress. The activity of JNK1 was decreased in both compartments by all three types of stress, while the activity of cytoplasmic JNK2/3 was elevated in acute and unaltered or lowered in chronic and combined stress. Under all stress conditions, Hsp70 translocation to the nucleus was markedly increased. The results suggest that neurodegenerative signaling of JNKs may be counteracted by increase of nuclear Hsp70, especially under chronic stress.",
journal = "Archives of Biological Sciences",
title = "Stress-Induced Phosphorylation of C-JUN-N-Terminal Kinases and Nuclear Translocation of Hsp70 in the Wistar Rat Hippocampus",
volume = "61",
number = "1",
pages = "1-8",
doi = "10.2298/ABS0901001A"
}

Adžić, M., Đorđević, A. D., Krstić-Demonacos, M.,& Radojčić, M.. (2009). Stress-Induced Phosphorylation of C-JUN-N-Terminal Kinases and Nuclear Translocation of Hsp70 in the Wistar Rat Hippocampus. in Archives of Biological Sciences, 61(1), 1-8.
https://doi.org/10.2298/ABS0901001A

Adžić M, Đorđević AD, Krstić-Demonacos M, Radojčić M. Stress-Induced Phosphorylation of C-JUN-N-Terminal Kinases and Nuclear Translocation of Hsp70 in the Wistar Rat Hippocampus. in Archives of Biological Sciences. 2009;61(1):1-8.
doi:10.2298/ABS0901001A .

Adžić, Miroslav, Đorđević, Ana D., Krstić-Demonacos, Marija, Radojčić, Marija, "Stress-Induced Phosphorylation of C-JUN-N-Terminal Kinases and Nuclear Translocation of Hsp70 in the Wistar Rat Hippocampus" in Archives of Biological Sciences, 61, no. 1 (2009):1-8,
https://doi.org/10.2298/ABS0901001A . .

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Radojčić, Marija
AU  - Demonacos, Constantinos
AU  - Krstić-Demonacos, Marija
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3667
AB  - The glucocorticoid receptor (GR) protein is a cytosolic ligand-dependent transcription factor with numerous functions regulated by post-translational modifications, including phosphorylation/dephosphorylation. Among the functions most extensively affected by GR phosphorylation are the modulation of its transcriptional activity, alterations in its interaction pattern with cofactors, nuclear translocation and selective gene transactivation. Intensive analysis of the intracellular distribution of GR phosphoisoforms and their interaction with proteins of other cellular signalling networks required the use of [gamma-(32)P]ATP as a phosphate donor, and special laboratory protection measures to avoid external irradiation and contamination. In the present study, simple and easy-to-use non-radioactive protein mobility shift assays (NMS assays) were developed using one- and/or two-dimensional gel electrophoresis based on differences in the pI and molecular mass of GR phosphoisoforms. The GR isoforms were immunodetected with specific monoclonal or polyclonal anti-GR antibodies by Western blot in three diverse systems, namely yeast BJ2168 cells expressing wild-type rat GR, rat hepatoma GRH2 cells grown in culture and brain tissue from Wistar rat experimental animals. The results obtained using the NMS assay were similar to previous results obtained with the [gamma-(32)P] ATP standard assay.
T2  - Journal of the Serbian Chemical Society
T1  - Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays
VL  - 74
IS  - 3
SP  - 237
EP  - 244
DO  - 10.2298/JSC0903237P
ER  - 

@article{
author = "Popović, Nataša M. and Nićiforović, Ana and Adžić, Miroslav and Radojčić, Marija and Demonacos, Constantinos and Krstić-Demonacos, Marija",
year = "2009",
abstract = "The glucocorticoid receptor (GR) protein is a cytosolic ligand-dependent transcription factor with numerous functions regulated by post-translational modifications, including phosphorylation/dephosphorylation. Among the functions most extensively affected by GR phosphorylation are the modulation of its transcriptional activity, alterations in its interaction pattern with cofactors, nuclear translocation and selective gene transactivation. Intensive analysis of the intracellular distribution of GR phosphoisoforms and their interaction with proteins of other cellular signalling networks required the use of [gamma-(32)P]ATP as a phosphate donor, and special laboratory protection measures to avoid external irradiation and contamination. In the present study, simple and easy-to-use non-radioactive protein mobility shift assays (NMS assays) were developed using one- and/or two-dimensional gel electrophoresis based on differences in the pI and molecular mass of GR phosphoisoforms. The GR isoforms were immunodetected with specific monoclonal or polyclonal anti-GR antibodies by Western blot in three diverse systems, namely yeast BJ2168 cells expressing wild-type rat GR, rat hepatoma GRH2 cells grown in culture and brain tissue from Wistar rat experimental animals. The results obtained using the NMS assay were similar to previous results obtained with the [gamma-(32)P] ATP standard assay.",
journal = "Journal of the Serbian Chemical Society",
title = "Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays",
volume = "74",
number = "3",
pages = "237-244",
doi = "10.2298/JSC0903237P"
}

Popović, N. M., Nićiforović, A., Adžić, M., Radojčić, M., Demonacos, C.,& Krstić-Demonacos, M.. (2009). Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays. in Journal of the Serbian Chemical Society, 74(3), 237-244.
https://doi.org/10.2298/JSC0903237P

Popović NM, Nićiforović A, Adžić M, Radojčić M, Demonacos C, Krstić-Demonacos M. Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays. in Journal of the Serbian Chemical Society. 2009;74(3):237-244.
doi:10.2298/JSC0903237P .

Popović, Nataša M., Nićiforović, Ana, Adžić, Miroslav, Radojčić, Marija, Demonacos, Constantinos, Krstić-Demonacos, Marija, "Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays" in Journal of the Serbian Chemical Society, 74, no. 3 (2009):237-244,
https://doi.org/10.2298/JSC0903237P . .