@conference{
author = "Pašić, Ivana and Preljević, Kenan and Matić, Ivana and Petrović, Nina and Stanojković, Tatjana and Perović, Svetlana",
year = "2023",
abstract = "Background: It has been demonstrated that aromaƟ c plant essenƟ al oils (EO) contain phytochemicals with anƟ - infl ammatory, anƟ oxidant, and anƟ cancer acƟ viƟ es. The goal of this study was to examine the cytotoxicity and the anƟ cancer acƟ on mechanisms of an EO extracted from Satureja montana plant species, originaƟ ng from Montenegro. Material and methods: The cytotoxic acƟ vity was assessed against human cancer cell lines: cervical adenocarcinoma HeLa, malignant melanoma A375, colorectal adenocarcinoma LS 174T, lung carcinoma A549, as well as against normal human lung fi broblasts MRC-5 by MTT assay. Using fl ow cytometry, it was examined how HeLa cells were distributed throughout the cell cycle aŌ er treatment with EO and whether caspase-3, caspase-8, and caspase-9 were acƟ vated. RT-qPCR was used to assess the expression levels of genes and miRNA in HeLa cells. Results: Satureja montana EO exerted strong cytotoxicity against human cancer cell lines, with IC50 values ranging from 0,12 to 0,18 µL/mL, The strongest cytotoxic acƟ vity of EO was observed against lung carcinoma A549 cells with an IC50 value of 0.12 µL/mL and LS 174T colorectal adenocarcinoma cells with IC50 value of 0.13 µL/mL. The lowest cytotoxicity was determined against normal lung fi broblasts MRC-5 (IC50 value of 0.19 µL/mL). AŌ er 24 hours of treatment with Satureja montana EO, a striking increase in the proporƟ on of HeLa cells in the subG1 phase was seen in comparison with control cells. Pretreatment of HeLa cells with caspase inhibitors showed that Satureja montana EO acƟ vated all three invesƟ gated caspases to trigger apoptosis. In comparison to control cells, Satureja montana EO treatment of HeLa cells decreased MMP2 gene expression levels, and elevated MMP9 and VEGFA gene expression levels. Satureja montana EO treatment increased the expression levels of the tumor supressive miR-16 and miR-34ain HeLa cells, and increased levels of miR21, with oncogenic role in cervical cancer. Conclusion: Satureja montana EO showed strong cytotoxic eff ects on tested cancer cells. EO exerted prominent apoptoƟ c acƟ vity in HeLa cells via intrinsic and extrinsic pathways. Decrease in the expression levels of MMP2 gene, involved in invasion and metastasis, and cancer suppressive miRNAs, further support its potenƟ al to be uƟ lized as a therapeuƟ c agent in cancer treatment.",
publisher = "Belgrade : Serbian Association for Cancer Research",
journal = "Oncology Insights",
title = "Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro",
number = "1",
pages = "67-68",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12566"
}
