Stanišić, Jelena

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  • Stanišić, Jelena (15)

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Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2

Stanišić, Jelena; Ivković, Tamara; Romić, Snježana Đ.; Zec, Manja; Ćulafić, Tijana; Stojiljković, Mojca D.; Korićanac, Goran

(2021)

TY  - JOUR
AU  - Stanišić, Jelena
AU  - Ivković, Tamara
AU  - Romić, Snježana Đ.
AU  - Zec, Manja
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca D.
AU  - Korićanac, Goran
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9110
AB  - Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.
T2  - International Journal of Food Sciences and Nutrition
T1  - Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2
VL  - 72
IS  - 3
SP  - 324
EP  - 334
DO  - 10.1080/09637486.2020.1796931
ER  - 
@article{
author = "Stanišić, Jelena and Ivković, Tamara and Romić, Snježana Đ. and Zec, Manja and Ćulafić, Tijana and Stojiljković, Mojca D. and Korićanac, Goran",
year = "2021",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/9110",
abstract = "Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.",
journal = "International Journal of Food Sciences and Nutrition",
title = "Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2",
volume = "72",
number = "3",
pages = "324-334",
doi = "10.1080/09637486.2020.1796931"
}
Stanišić, J., Ivković, T., Romić, S. Đ., Zec, M., Ćulafić, T., Stojiljković, M. D.,& Korićanac, G. (2021). Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2.
International Journal of Food Sciences and Nutrition, 72(3), 324-334.
https://doi.org/10.1080/09637486.2020.1796931
Stanišić J, Ivković T, Romić SĐ, Zec M, Ćulafić T, Stojiljković MD, Korićanac G. Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2. International Journal of Food Sciences and Nutrition. 2021;72(3):324-334
Stanišić Jelena, Ivković Tamara, Romić Snježana Đ., Zec Manja, Ćulafić Tijana, Stojiljković Mojca D., Korićanac Goran, "Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2" 72, no. 3 (2021):324-334,
https://doi.org/10.1080/09637486.2020.1796931 .
3
2
1

Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2

Stanišić, Jelena; Ivković, Tamara; Romić, Snježana Đ.; Zec, Manja; Ćulafić, Tijana; Stojiljković, Mojca D.; Korićanac, Goran

(2021)

TY  - JOUR
AU  - Stanišić, Jelena
AU  - Ivković, Tamara
AU  - Romić, Snježana Đ.
AU  - Zec, Manja
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca D.
AU  - Korićanac, Goran
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9103
AB  - Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.
T2  - International Journal of Food Sciences and Nutrition
T1  - Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2
VL  - 72
IS  - 3
SP  - 324
EP  - 334
DO  - 10.1080/09637486.2020.1796931
ER  - 
@article{
author = "Stanišić, Jelena and Ivković, Tamara and Romić, Snježana Đ. and Zec, Manja and Ćulafić, Tijana and Stojiljković, Mojca D. and Korićanac, Goran",
year = "2021",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/9103",
abstract = "Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.",
journal = "International Journal of Food Sciences and Nutrition",
title = "Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2",
volume = "72",
number = "3",
pages = "324-334",
doi = "10.1080/09637486.2020.1796931"
}
Stanišić, J., Ivković, T., Romić, S. Đ., Zec, M., Ćulafić, T., Stojiljković, M. D.,& Korićanac, G. (2021). Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2.
International Journal of Food Sciences and Nutrition, 72(3), 324-334.
https://doi.org/10.1080/09637486.2020.1796931
Stanišić J, Ivković T, Romić SĐ, Zec M, Ćulafić T, Stojiljković MD, Korićanac G. Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2. International Journal of Food Sciences and Nutrition. 2021;72(3):324-334
Stanišić Jelena, Ivković Tamara, Romić Snježana Đ., Zec Manja, Ćulafić Tijana, Stojiljković Mojca D., Korićanac Goran, "Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2" 72, no. 3 (2021):324-334,
https://doi.org/10.1080/09637486.2020.1796931 .
3
2
1

Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats

Romić, Snježana Đ.; Đorđević, Ana; Tepavčević, Snežana; Ćulafić, Tijana; Stojiljković, Mojca D.; Bursać, Biljana; Stanišić, Jelena; Kostić, Milan; Gligorovska, Ljupka; Korićanac, Goran

(2020)

TY  - JOUR
AU  - Romić, Snježana Đ.
AU  - Đorđević, Ana
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca D.
AU  - Bursać, Biljana
AU  - Stanišić, Jelena
AU  - Kostić, Milan
AU  - Gligorovska, Ljupka
AU  - Korićanac, Goran
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8849
AB  - Both a diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine the effects of the fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether these effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress for 9 weeks. Protein expression and/or phosphorylation of the insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by western blot. GR distribution between cytosolic and nuclear fractions was also analyzed. The fructose-rich diet decreased the level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased the IRS1 content and prevented the fructose diet-induced decrease of the pAkt (Ser473) level. The fructose-rich diet in combination with chronic stress reduced the protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased the protein content of Na+/K+-ATPase α1- and α2-subunits, while after combined treatment the α2 content was at the control level and the α1 content was lower than the control level. The effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights into the effects of chronic stress and a combination of the fructose diet and chronic stress on the studied molecules in the heart.
T2  - Food & Function
T1  - Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats
VL  - 11
IS  - 2
SP  - 1455
EP  - 1466
DO  - 10.1039/C9FO02306B
ER  - 
@article{
author = "Romić, Snježana Đ. and Đorđević, Ana and Tepavčević, Snežana and Ćulafić, Tijana and Stojiljković, Mojca D. and Bursać, Biljana and Stanišić, Jelena and Kostić, Milan and Gligorovska, Ljupka and Korićanac, Goran",
year = "2020",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/8849",
abstract = "Both a diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine the effects of the fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether these effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress for 9 weeks. Protein expression and/or phosphorylation of the insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by western blot. GR distribution between cytosolic and nuclear fractions was also analyzed. The fructose-rich diet decreased the level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased the IRS1 content and prevented the fructose diet-induced decrease of the pAkt (Ser473) level. The fructose-rich diet in combination with chronic stress reduced the protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased the protein content of Na+/K+-ATPase α1- and α2-subunits, while after combined treatment the α2 content was at the control level and the α1 content was lower than the control level. The effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights into the effects of chronic stress and a combination of the fructose diet and chronic stress on the studied molecules in the heart.",
journal = "Food & Function",
title = "Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats",
volume = "11",
number = "2",
pages = "1455-1466",
doi = "10.1039/C9FO02306B"
}
Romić, S. Đ., Đorđević, A., Tepavčević, S., Ćulafić, T., Stojiljković, M. D., Bursać, B., Stanišić, J., Kostić, M., Gligorovska, L.,& Korićanac, G. (2020). Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats.
Food & Function, 11(2), 1455-1466.
https://doi.org/10.1039/C9FO02306B
Romić SĐ, Đorđević A, Tepavčević S, Ćulafić T, Stojiljković MD, Bursać B, Stanišić J, Kostić M, Gligorovska L, Korićanac G. Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats. Food & Function. 2020;11(2):1455-1466
Romić Snježana Đ., Đorđević Ana, Tepavčević Snežana, Ćulafić Tijana, Stojiljković Mojca D., Bursać Biljana, Stanišić Jelena, Kostić Milan, Gligorovska Ljupka, Korićanac Goran, "Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats" 11, no. 2 (2020):1455-1466,
https://doi.org/10.1039/C9FO02306B .
1
1
1

The role of the adenosine triphosphate-sensitive potassium channels in pinacidil-induced vasodilatation of the human saphenous vein in patients with and without type 2 diabetes mellitus

Rajković, Jovana; Perić, M.; Stanišić, Jelena; Novaković, Radmila; Đokić, Vladimir; Rakočević, Jelena; Tepavčević, Snežana; Labudović-Borović, Milica; Gostimirović, Miloš; Heinle, Helmut; Gojković-Bukarica, Ljiljana

(2020)

TY  - JOUR
AU  - Rajković, Jovana
AU  - Perić, M.
AU  - Stanišić, Jelena
AU  - Novaković, Radmila
AU  - Đokić, Vladimir
AU  - Rakočević, Jelena
AU  - Tepavčević, Snežana
AU  - Labudović-Borović, Milica
AU  - Gostimirović, Miloš
AU  - Heinle, Helmut
AU  - Gojković-Bukarica, Ljiljana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9054
AB  - Type 2 diabetes mellitus (T2DM) increases cardiovascular complications. Diabetic vascular dysfunction is associated with the reduced activity of the different smooth muscle potassium (K+) channels. Thus, the objective of our study was to investigate the role of the adenosine triphosphate (ATP)-sensitive K+ (KATP) channels in the relaxant effect of potassium channel opener, pinacidil on the human saphenous vein (HSV) obtained from the patients with and without T2DM. The rings of HSV without the endothelium, obtained from the patients who had undergone coronary bypass surgery, were mounted in an organ bath system and isometric tension was recorded. The relaxation of HSV, precontracted with phenylephrine, was produced by pinacidil. The expression of KATP subunits (Kir6.1, Kir6.2 and SUR2B) was detected by immunohistochemistry and Western blot. Pinacidil produces comparable effects on HSV in patients with and without T2DM. The suppression of pinacidil effect and its maximal relaxation by glibenclamide, selective blocker of KATP channels, was more pronounced on HSV in patients without T2DM. All three types of KATP subunits are expressed on the smooth muscle cells of HSV. While there are no differences in the expression of Kir6.1 and Kir6.2, the expression of SUR2B is lower in HSV in patients with T2DM. Pinacidil produced comparable KATP-dependent and -independent relaxation of the HSV in patients with/without T2DM. According to the effect of glibenclamide and the applied molecular analysis, presented findings demonstrated that diabetes mellitus was associated with the reduced expression of SUR2B subunit in the vascular smooth muscle of HSV.
T2  - Journal of physiology and pharmacology : an official journal of the Polish Physiological Society
T1  - The role of the adenosine triphosphate-sensitive potassium channels in pinacidil-induced vasodilatation of the human saphenous vein in patients with and without type 2 diabetes mellitus
VL  - 71
IS  - 1
DO  - 10.26402/jpp.2020.1.12
ER  - 
@article{
author = "Rajković, Jovana and Perić, M. and Stanišić, Jelena and Novaković, Radmila and Đokić, Vladimir and Rakočević, Jelena and Tepavčević, Snežana and Labudović-Borović, Milica and Gostimirović, Miloš and Heinle, Helmut and Gojković-Bukarica, Ljiljana",
year = "2020",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/9054",
abstract = "Type 2 diabetes mellitus (T2DM) increases cardiovascular complications. Diabetic vascular dysfunction is associated with the reduced activity of the different smooth muscle potassium (K+) channels. Thus, the objective of our study was to investigate the role of the adenosine triphosphate (ATP)-sensitive K+ (KATP) channels in the relaxant effect of potassium channel opener, pinacidil on the human saphenous vein (HSV) obtained from the patients with and without T2DM. The rings of HSV without the endothelium, obtained from the patients who had undergone coronary bypass surgery, were mounted in an organ bath system and isometric tension was recorded. The relaxation of HSV, precontracted with phenylephrine, was produced by pinacidil. The expression of KATP subunits (Kir6.1, Kir6.2 and SUR2B) was detected by immunohistochemistry and Western blot. Pinacidil produces comparable effects on HSV in patients with and without T2DM. The suppression of pinacidil effect and its maximal relaxation by glibenclamide, selective blocker of KATP channels, was more pronounced on HSV in patients without T2DM. All three types of KATP subunits are expressed on the smooth muscle cells of HSV. While there are no differences in the expression of Kir6.1 and Kir6.2, the expression of SUR2B is lower in HSV in patients with T2DM. Pinacidil produced comparable KATP-dependent and -independent relaxation of the HSV in patients with/without T2DM. According to the effect of glibenclamide and the applied molecular analysis, presented findings demonstrated that diabetes mellitus was associated with the reduced expression of SUR2B subunit in the vascular smooth muscle of HSV.",
journal = "Journal of physiology and pharmacology : an official journal of the Polish Physiological Society",
title = "The role of the adenosine triphosphate-sensitive potassium channels in pinacidil-induced vasodilatation of the human saphenous vein in patients with and without type 2 diabetes mellitus",
volume = "71",
number = "1",
doi = "10.26402/jpp.2020.1.12"
}
Rajković, J., Perić, M., Stanišić, J., Novaković, R., Đokić, V., Rakočević, J., Tepavčević, S., Labudović-Borović, M., Gostimirović, M., Heinle, H.,& Gojković-Bukarica, L. (2020). The role of the adenosine triphosphate-sensitive potassium channels in pinacidil-induced vasodilatation of the human saphenous vein in patients with and without type 2 diabetes mellitus.
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 71(1).
https://doi.org/10.26402/jpp.2020.1.12
Rajković J, Perić M, Stanišić J, Novaković R, Đokić V, Rakočević J, Tepavčević S, Labudović-Borović M, Gostimirović M, Heinle H, Gojković-Bukarica L. The role of the adenosine triphosphate-sensitive potassium channels in pinacidil-induced vasodilatation of the human saphenous vein in patients with and without type 2 diabetes mellitus. Journal of physiology and pharmacology : an official journal of the Polish Physiological Society. 2020;71(1)
Rajković Jovana, Perić M., Stanišić Jelena, Novaković Radmila, Đokić Vladimir, Rakočević Jelena, Tepavčević Snežana, Labudović-Borović Milica, Gostimirović Miloš, Heinle Helmut, Gojković-Bukarica Ljiljana, "The role of the adenosine triphosphate-sensitive potassium channels in pinacidil-induced vasodilatation of the human saphenous vein in patients with and without type 2 diabetes mellitus" 71, no. 1 (2020),
https://doi.org/10.26402/jpp.2020.1.12 .

Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle

Đokić, Vladimir; Janković, Svetlana; Labudović-Borović, Milica; Rakočević, Jelena; Stanišić, Jelena; Rajković, Jovana; Novaković, Radmila; Kostić, Milan; Đurić, Miloš; Gostimirović, Miloš; Gojković-Bukarica, Ljiljana

(2020)

TY  - JOUR
AU  - Đokić, Vladimir
AU  - Janković, Svetlana
AU  - Labudović-Borović, Milica
AU  - Rakočević, Jelena
AU  - Stanišić, Jelena
AU  - Rajković, Jovana
AU  - Novaković, Radmila
AU  - Kostić, Milan
AU  - Đurić, Miloš
AU  - Gostimirović, Miloš
AU  - Gojković-Bukarica, Ljiljana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9048
AB  - Voltage-gated potassium (K ) channels are the largest superfamily of potassium (K) channels. A variety of K channels are expressed in the vascular smooth muscle cells (SMC). Studies have shown that gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) cause various changes in the human umbilical vein (HUV). Recently, we have shown that 4-AP, a nonspecific K 1-4 channel inhibitor, significantly decreases vasorelaxation induced by K channel opener pinacidil in vascular SMCs of the HUVs from normal pregnancies, but not in GDM and PIH. The goal of this study was to provide more detailed insight in the K channel subtypes involved in pinacidil-induced vasodilation of HUVs, as well as to investigate potential alterations of their function and expression during GDM and PIH. Margatoxin, a specific blocker of K 1.2 and K 1.3 channels, significantly antagonized pinacidil-induced vasorelaxation in normal pregnancy, while in HUVs from GDM and PIH that was not the case, indicating damage of K 1.2 and K 1.3 channel function. Immunohistochemistry and Western blot revealed similar expression of K 1.2 channels in all groups. The expression of K 1.3 subunit was significantly decreased in PIH, while it remained unchanged in GDM compared to normal pregnancy. Phrixotoxin, specific blocker of K 4.2 and K 4.3 channels, did not antagonize response to pinacidil in any of the groups. The major novel findings show that margatoxin antagonized pinacidil-induced relaxation in normal pregnancy, but not in GDM and PIH. Decreased expression of K 1.3 channels in HUV during PIH may be important pathophysiological mechanism contributing to an increased risk of adverse pregnancy outcomes.
T2  - European Journal of Pharmacology
T1  - Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle
VL  - 882
SP  - 173281
DO  - 10.1016/j.ejphar.2020.173281
ER  - 
@article{
author = "Đokić, Vladimir and Janković, Svetlana and Labudović-Borović, Milica and Rakočević, Jelena and Stanišić, Jelena and Rajković, Jovana and Novaković, Radmila and Kostić, Milan and Đurić, Miloš and Gostimirović, Miloš and Gojković-Bukarica, Ljiljana",
year = "2020",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/9048",
abstract = "Voltage-gated potassium (K ) channels are the largest superfamily of potassium (K) channels. A variety of K channels are expressed in the vascular smooth muscle cells (SMC). Studies have shown that gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) cause various changes in the human umbilical vein (HUV). Recently, we have shown that 4-AP, a nonspecific K 1-4 channel inhibitor, significantly decreases vasorelaxation induced by K channel opener pinacidil in vascular SMCs of the HUVs from normal pregnancies, but not in GDM and PIH. The goal of this study was to provide more detailed insight in the K channel subtypes involved in pinacidil-induced vasodilation of HUVs, as well as to investigate potential alterations of their function and expression during GDM and PIH. Margatoxin, a specific blocker of K 1.2 and K 1.3 channels, significantly antagonized pinacidil-induced vasorelaxation in normal pregnancy, while in HUVs from GDM and PIH that was not the case, indicating damage of K 1.2 and K 1.3 channel function. Immunohistochemistry and Western blot revealed similar expression of K 1.2 channels in all groups. The expression of K 1.3 subunit was significantly decreased in PIH, while it remained unchanged in GDM compared to normal pregnancy. Phrixotoxin, specific blocker of K 4.2 and K 4.3 channels, did not antagonize response to pinacidil in any of the groups. The major novel findings show that margatoxin antagonized pinacidil-induced relaxation in normal pregnancy, but not in GDM and PIH. Decreased expression of K 1.3 channels in HUV during PIH may be important pathophysiological mechanism contributing to an increased risk of adverse pregnancy outcomes.",
journal = "European Journal of Pharmacology",
title = "Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle",
volume = "882",
pages = "173281",
doi = "10.1016/j.ejphar.2020.173281"
}
Đokić, V., Janković, S., Labudović-Borović, M., Rakočević, J., Stanišić, J., Rajković, J., Novaković, R., Kostić, M., Đurić, M., Gostimirović, M.,& Gojković-Bukarica, L. (2020). Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle.
European Journal of Pharmacology, 882, 173281.
https://doi.org/10.1016/j.ejphar.2020.173281
Đokić V, Janković S, Labudović-Borović M, Rakočević J, Stanišić J, Rajković J, Novaković R, Kostić M, Đurić M, Gostimirović M, Gojković-Bukarica L. Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle. European Journal of Pharmacology. 2020;882:173281
Đokić Vladimir, Janković Svetlana, Labudović-Borović Milica, Rakočević Jelena, Stanišić Jelena, Rajković Jovana, Novaković Radmila, Kostić Milan, Đurić Miloš, Gostimirović Miloš, Gojković-Bukarica Ljiljana, "Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle" 882 (2020):173281,
https://doi.org/10.1016/j.ejphar.2020.173281 .
1
1

Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels

Đokić, Vladimir; Janković-Ražnatović, Svetlana; Novaković, Radmila; Kostić, Milan; Rajković, Jovana; Labudović-Borović, Milica; Rakočević, Jelena T.; Stanišić, Jelena; Đurić, Miloš; Gojković-Bukarica, Ljiljana

(2019)

TY  - JOUR
AU  - Đokić, Vladimir
AU  - Janković-Ražnatović, Svetlana
AU  - Novaković, Radmila
AU  - Kostić, Milan
AU  - Rajković, Jovana
AU  - Labudović-Borović, Milica
AU  - Rakočević, Jelena T.
AU  - Stanišić, Jelena
AU  - Đurić, Miloš
AU  - Gojković-Bukarica, Ljiljana
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8626
AB  - Gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) can jeopardize mother and/or fetus. Vascular ATP-sensitive potassium (KATP) channels most likely participate in the processes of diabetes and hypertension. The aim of this research was to examine whether GDM and PIH cause changes in the expression and function of KATP channels in vascular smooth muscle of human umbilical vein (HUV). Western blot and immunohistochemistry detected significantly decreased expression of Kir6.1 subunit of KATP channels in GDM and PIH, while the expression of SUR2B was unchanged. In GDM, a K+ channel opener, pinacidil caused reduced relaxation of the endothelium-denuded HUVs compared to normal pregnancy. However, its effects in HUVs from PIH subjects were similar to normal pregnancy. In all groups KATP channel blocker glibenclamide antagonized the relaxation of HUV induced by pinacidil without change in the maximal relaxations indicating additional KATP channel-independent mechanisms of pinacidil action. Iberiotoxin, a selective antagonist of large-conductance calcium-activated potassium channels, inhibited the relaxant effect of pinacidil in PIH, but not in normal pregnancy and GDM. Experiments performed in K+-rich solution confirmed the existence of K+-independent effects of pinacidil, which also appear to be impaired in GDM and PIH. Thus, the expression of KATP channels is decreased in GDM and PIH. In GDM, vasorelaxant response of HUV to pinacidil is reduced, while in PIH it remains unchanged. It is very likely that KATP channels modulation and more detailed insight in KATP channel-independent actions of pinacidil may be precious in the therapy of pathological pregnancies. © 2019 Elsevier Inc.
T2  - Experimental and Molecular Pathology
T1  - Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels
VL  - 111
SP  - 104323
DO  - 10.1016/j.yexmp.2019.104323
ER  - 
@article{
author = "Đokić, Vladimir and Janković-Ražnatović, Svetlana and Novaković, Radmila and Kostić, Milan and Rajković, Jovana and Labudović-Borović, Milica and Rakočević, Jelena T. and Stanišić, Jelena and Đurić, Miloš and Gojković-Bukarica, Ljiljana",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8626",
abstract = "Gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) can jeopardize mother and/or fetus. Vascular ATP-sensitive potassium (KATP) channels most likely participate in the processes of diabetes and hypertension. The aim of this research was to examine whether GDM and PIH cause changes in the expression and function of KATP channels in vascular smooth muscle of human umbilical vein (HUV). Western blot and immunohistochemistry detected significantly decreased expression of Kir6.1 subunit of KATP channels in GDM and PIH, while the expression of SUR2B was unchanged. In GDM, a K+ channel opener, pinacidil caused reduced relaxation of the endothelium-denuded HUVs compared to normal pregnancy. However, its effects in HUVs from PIH subjects were similar to normal pregnancy. In all groups KATP channel blocker glibenclamide antagonized the relaxation of HUV induced by pinacidil without change in the maximal relaxations indicating additional KATP channel-independent mechanisms of pinacidil action. Iberiotoxin, a selective antagonist of large-conductance calcium-activated potassium channels, inhibited the relaxant effect of pinacidil in PIH, but not in normal pregnancy and GDM. Experiments performed in K+-rich solution confirmed the existence of K+-independent effects of pinacidil, which also appear to be impaired in GDM and PIH. Thus, the expression of KATP channels is decreased in GDM and PIH. In GDM, vasorelaxant response of HUV to pinacidil is reduced, while in PIH it remains unchanged. It is very likely that KATP channels modulation and more detailed insight in KATP channel-independent actions of pinacidil may be precious in the therapy of pathological pregnancies. © 2019 Elsevier Inc.",
journal = "Experimental and Molecular Pathology",
title = "Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels",
volume = "111",
pages = "104323",
doi = "10.1016/j.yexmp.2019.104323"
}
Đokić, V., Janković-Ražnatović, S., Novaković, R., Kostić, M., Rajković, J., Labudović-Borović, M., Rakočević, J. T., Stanišić, J., Đurić, M.,& Gojković-Bukarica, L. (2019). Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels.
Experimental and Molecular Pathology, 111, 104323.
https://doi.org/10.1016/j.yexmp.2019.104323
Đokić V, Janković-Ražnatović S, Novaković R, Kostić M, Rajković J, Labudović-Borović M, Rakočević JT, Stanišić J, Đurić M, Gojković-Bukarica L. Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels. Experimental and Molecular Pathology. 2019;111:104323
Đokić Vladimir, Janković-Ražnatović Svetlana, Novaković Radmila, Kostić Milan, Rajković Jovana, Labudović-Borović Milica, Rakočević Jelena T., Stanišić Jelena, Đurić Miloš, Gojković-Bukarica Ljiljana, "Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels" 111 (2019):104323,
https://doi.org/10.1016/j.yexmp.2019.104323 .
1
6
4
3

Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats

Bošković, Maja; Bundalo, Maja M.; Živković, Maja; Stanišić, Jelena; Kostić, Milan; Korićanac, Goran; Stanković, Aleksandra

(2019)

TY  - JOUR
AU  - Bošković, Maja
AU  - Bundalo, Maja M.
AU  - Živković, Maja
AU  - Stanišić, Jelena
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7984
AB  - Harmful effects of fructose-rich diet (FRD) were predominantly observed in males, suggesting protective effects of estrogens. Little is known about AMPK/sirtuin-1 (SIRT1)/forkhead box O3 (FoxO3a)/manganese superoxide dismutase (MnSOD)/catalase signaling in the heart in state of metabolic syndrome and oxidative stress induced by fructose over-consumption. We investigated the effect of 10% FRD on expression of AMPK-SIRT1-FoxO3a-MnSOD/catalase axis in myocardium and potentially beneficial effect of 17β-estradiol replacement. The expression of NADPH oxidase 4 (Nox4) and miRNA-155, unfavorable regulators of this axis, were also investigated. FRD significantly increased AMPK and decreased FoxO3a activity, decreased SIRT1, MnSOD and Nox4 protein expression while E2 reverted these changes, except for Nox4, and increased catalase protein level. E2 diminished Nox4 and MnSOD mRNA level in FRD ovariectomized rats. These results suggest independent response of AMPK and SIRT to FRD treatment. The proposed signaling in the heart should be further investigated in the prooxidative and antioxidative milieu.
T2  - Journal of Functional Foods
T1  - Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats
VL  - 52
SP  - 690
EP  - 698
DO  - 10.1016/j.jff.2018.11.053
ER  - 
@article{
author = "Bošković, Maja and Bundalo, Maja M. and Živković, Maja and Stanišić, Jelena and Kostić, Milan and Korićanac, Goran and Stanković, Aleksandra",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7984",
abstract = "Harmful effects of fructose-rich diet (FRD) were predominantly observed in males, suggesting protective effects of estrogens. Little is known about AMPK/sirtuin-1 (SIRT1)/forkhead box O3 (FoxO3a)/manganese superoxide dismutase (MnSOD)/catalase signaling in the heart in state of metabolic syndrome and oxidative stress induced by fructose over-consumption. We investigated the effect of 10% FRD on expression of AMPK-SIRT1-FoxO3a-MnSOD/catalase axis in myocardium and potentially beneficial effect of 17β-estradiol replacement. The expression of NADPH oxidase 4 (Nox4) and miRNA-155, unfavorable regulators of this axis, were also investigated. FRD significantly increased AMPK and decreased FoxO3a activity, decreased SIRT1, MnSOD and Nox4 protein expression while E2 reverted these changes, except for Nox4, and increased catalase protein level. E2 diminished Nox4 and MnSOD mRNA level in FRD ovariectomized rats. These results suggest independent response of AMPK and SIRT to FRD treatment. The proposed signaling in the heart should be further investigated in the prooxidative and antioxidative milieu.",
journal = "Journal of Functional Foods",
title = "Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats",
volume = "52",
pages = "690-698",
doi = "10.1016/j.jff.2018.11.053"
}
Bošković, M., Bundalo, M. M., Živković, M., Stanišić, J., Kostić, M., Korićanac, G.,& Stanković, A. (2019). Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats.
Journal of Functional Foods, 52, 690-698.
https://doi.org/10.1016/j.jff.2018.11.053
Bošković M, Bundalo MM, Živković M, Stanišić J, Kostić M, Korićanac G, Stanković A. Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats. Journal of Functional Foods. 2019;52:690-698
Bošković Maja, Bundalo Maja M., Živković Maja, Stanišić Jelena, Kostić Milan, Korićanac Goran, Stanković Aleksandra, "Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats" 52 (2019):690-698,
https://doi.org/10.1016/j.jff.2018.11.053 .
4
2
3

Involvement of large-conductance calcium-activated potassium channels in pinacidil effects on the isolated bypass grafts from patients with and without type-2 diabetes mellitus

Rajković, Jovana; Perić, Miodrag; Stanišić, Jelena; Rakočević, Jelena T.; Novaković, Radmila; Đokić, Vladimir; Labudović-Borović, Milica; Tepavčević, Snežana; Heinle, Helmut; Gojković-Bukarica, Ljiljana

(2018)

TY  - CONF
AU  - Rajković, Jovana
AU  - Perić, Miodrag
AU  - Stanišić, Jelena
AU  - Rakočević, Jelena T.
AU  - Novaković, Radmila
AU  - Đokić, Vladimir
AU  - Labudović-Borović, Milica
AU  - Tepavčević, Snežana
AU  - Heinle, Helmut
AU  - Gojković-Bukarica, Ljiljana
PY  - 2018
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7912
C3  - European Journal of Clinical Investigation
T1  - Involvement of large-conductance calcium-activated potassium channels in pinacidil effects on the isolated bypass grafts from patients with and without type-2 diabetes mellitus
VL  - 48
IS  - SI (Suppl. 1)
SP  - 137
ER  - 
@conference{
author = "Rajković, Jovana and Perić, Miodrag and Stanišić, Jelena and Rakočević, Jelena T. and Novaković, Radmila and Đokić, Vladimir and Labudović-Borović, Milica and Tepavčević, Snežana and Heinle, Helmut and Gojković-Bukarica, Ljiljana",
year = "2018",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7912",
journal = "European Journal of Clinical Investigation",
title = "Involvement of large-conductance calcium-activated potassium channels in pinacidil effects on the isolated bypass grafts from patients with and without type-2 diabetes mellitus",
volume = "48",
number = "SI (Suppl. 1)",
pages = "137"
}
Rajković, J., Perić, M., Stanišić, J., Rakočević, J. T., Novaković, R., Đokić, V., Labudović-Borović, M., Tepavčević, S., Heinle, H.,& Gojković-Bukarica, L. (2018). Involvement of large-conductance calcium-activated potassium channels in pinacidil effects on the isolated bypass grafts from patients with and without type-2 diabetes mellitus.
European Journal of Clinical Investigation, 48(SI (Suppl. 1)), 137.
Rajković J, Perić M, Stanišić J, Rakočević JT, Novaković R, Đokić V, Labudović-Borović M, Tepavčević S, Heinle H, Gojković-Bukarica L. Involvement of large-conductance calcium-activated potassium channels in pinacidil effects on the isolated bypass grafts from patients with and without type-2 diabetes mellitus. European Journal of Clinical Investigation. 2018;48(SI (Suppl. 1)):137
Rajković Jovana, Perić Miodrag, Stanišić Jelena, Rakočević Jelena T., Novaković Radmila, Đokić Vladimir, Labudović-Borović Milica, Tepavčević Snežana, Heinle Helmut, Gojković-Bukarica Ljiljana, "Involvement of large-conductance calcium-activated potassium channels in pinacidil effects on the isolated bypass grafts from patients with and without type-2 diabetes mellitus" 48, no. SI (Suppl. 1) (2018):137

Involvement of atp-sensitive and large-condutance calcium-activated potassium channels in pinacidil effects on the isolated internal mammary artery grafts from patients with type-2 diabetes mellitus

Rajković, Jovana; Perić, Miodrag; Stanišić, Jelena; Rakočević, Jelena T.; Novaković, Radmila; Đokić, Vladimir; Labudović-Borović, Milica; Tepavčević, Snežana; Kanjuh, Vladimir; Heinle, Helmut; Gojković-Bukarica, Ljiljana

(2018)

TY  - CONF
AU  - Rajković, Jovana
AU  - Perić, Miodrag
AU  - Stanišić, Jelena
AU  - Rakočević, Jelena T.
AU  - Novaković, Radmila
AU  - Đokić, Vladimir
AU  - Labudović-Borović, Milica
AU  - Tepavčević, Snežana
AU  - Kanjuh, Vladimir
AU  - Heinle, Helmut
AU  - Gojković-Bukarica, Ljiljana
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0021915018307019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7949
C3  - Atherosclerosis
T1  - Involvement of atp-sensitive and large-condutance calcium-activated potassium channels in pinacidil effects on the isolated internal mammary artery grafts from patients with type-2 diabetes mellitus
VL  - 275
SP  - e133
DO  - 10.1016/j.atherosclerosis.2018.06.389
ER  - 
@conference{
author = "Rajković, Jovana and Perić, Miodrag and Stanišić, Jelena and Rakočević, Jelena T. and Novaković, Radmila and Đokić, Vladimir and Labudović-Borović, Milica and Tepavčević, Snežana and Kanjuh, Vladimir and Heinle, Helmut and Gojković-Bukarica, Ljiljana",
year = "2018",
url = "https://linkinghub.elsevier.com/retrieve/pii/S0021915018307019, http://vinar.vin.bg.ac.rs/handle/123456789/7949",
journal = "Atherosclerosis",
title = "Involvement of atp-sensitive and large-condutance calcium-activated potassium channels in pinacidil effects on the isolated internal mammary artery grafts from patients with type-2 diabetes mellitus",
volume = "275",
pages = "e133",
doi = "10.1016/j.atherosclerosis.2018.06.389"
}
Rajković, J., Perić, M., Stanišić, J., Rakočević, J. T., Novaković, R., Đokić, V., Labudović-Borović, M., Tepavčević, S., Kanjuh, V., Heinle, H.,& Gojković-Bukarica, L. (2018). Involvement of atp-sensitive and large-condutance calcium-activated potassium channels in pinacidil effects on the isolated internal mammary artery grafts from patients with type-2 diabetes mellitus.
Atherosclerosis, 275, e133.
https://doi.org/10.1016/j.atherosclerosis.2018.06.389
Rajković J, Perić M, Stanišić J, Rakočević JT, Novaković R, Đokić V, Labudović-Borović M, Tepavčević S, Kanjuh V, Heinle H, Gojković-Bukarica L. Involvement of atp-sensitive and large-condutance calcium-activated potassium channels in pinacidil effects on the isolated internal mammary artery grafts from patients with type-2 diabetes mellitus. Atherosclerosis. 2018;275:e133
Rajković Jovana, Perić Miodrag, Stanišić Jelena, Rakočević Jelena T., Novaković Radmila, Đokić Vladimir, Labudović-Borović Milica, Tepavčević Snežana, Kanjuh Vladimir, Heinle Helmut, Gojković-Bukarica Ljiljana, "Involvement of atp-sensitive and large-condutance calcium-activated potassium channels in pinacidil effects on the isolated internal mammary artery grafts from patients with type-2 diabetes mellitus" 275 (2018):e133,
https://doi.org/10.1016/j.atherosclerosis.2018.06.389 .

Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats

Stanišić, Jelena; Korićanac, Goran; Stojiljković, Mirjana; Ćulafić, Tijana; Kostić, Mirjana M.; Romić, Snježana Đ.; Pantelić, Marija; Tepavčević, Snežana

(2018)

TY  - CONF
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Stojiljković, Mirjana
AU  - Ćulafić, Tijana
AU  - Kostić, Mirjana M.
AU  - Romić, Snježana Đ.
AU  - Pantelić, Marija
AU  - Tepavčević, Snežana
PY  - 2018
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7920
C3  - FEBS OPEN BIO
T1  - Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats
VL  - 8
IS  - Supplement 1
SP  - 224
DO  - 10.1002/2211-5463.12453
ER  - 
@conference{
author = "Stanišić, Jelena and Korićanac, Goran and Stojiljković, Mirjana and Ćulafić, Tijana and Kostić, Mirjana M. and Romić, Snježana Đ. and Pantelić, Marija and Tepavčević, Snežana",
year = "2018",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7920",
journal = "FEBS OPEN BIO",
title = "Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats",
volume = "8",
number = "Supplement 1",
pages = "224",
doi = "10.1002/2211-5463.12453"
}
Stanišić, J., Korićanac, G., Stojiljković, M., Ćulafić, T., Kostić, M. M., Romić, S. Đ., Pantelić, M.,& Tepavčević, S. (2018). Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats.
FEBS OPEN BIO, 8(Supplement 1), 224.
https://doi.org/10.1002/2211-5463.12453
Stanišić J, Korićanac G, Stojiljković M, Ćulafić T, Kostić MM, Romić SĐ, Pantelić M, Tepavčević S. Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats. FEBS OPEN BIO. 2018;8(Supplement 1):224
Stanišić Jelena, Korićanac Goran, Stojiljković Mirjana, Ćulafić Tijana, Kostić Mirjana M., Romić Snježana Đ., Pantelić Marija, Tepavčević Snežana, "Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats" 8, no. Supplement 1 (2018):224,
https://doi.org/10.1002/2211-5463.12453 .
18
10

Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet

Kostić, Mirjana M.; Korićanac, Goran; Tepavčević, Snežana; Ćulafić, Tijana; Romić, Snježana Đ.; Stanišić, Jelena; Pantelić, Marija; Stojiljković, Mirjana

(2018)

TY  - CONF
AU  - Kostić, Mirjana M.
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Stanišić, Jelena
AU  - Pantelić, Marija
AU  - Stojiljković, Mirjana
PY  - 2018
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7922
C3  - FEBS OPEN BIO
T1  - Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet
VL  - 8
IS  - Supplement 1
SP  - 223
DO  - 10.1002/2211-5463.12453
ER  - 
@conference{
author = "Kostić, Mirjana M. and Korićanac, Goran and Tepavčević, Snežana and Ćulafić, Tijana and Romić, Snježana Đ. and Stanišić, Jelena and Pantelić, Marija and Stojiljković, Mirjana",
year = "2018",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7922",
journal = "FEBS OPEN BIO",
title = "Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet",
volume = "8",
number = "Supplement 1",
pages = "223",
doi = "10.1002/2211-5463.12453"
}
Kostić, M. M., Korićanac, G., Tepavčević, S., Ćulafić, T., Romić, S. Đ., Stanišić, J., Pantelić, M.,& Stojiljković, M. (2018). Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet.
FEBS OPEN BIO, 8(Supplement 1), 223.
https://doi.org/10.1002/2211-5463.12453
Kostić MM, Korićanac G, Tepavčević S, Ćulafić T, Romić SĐ, Stanišić J, Pantelić M, Stojiljković M. Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet. FEBS OPEN BIO. 2018;8(Supplement 1):223
Kostić Mirjana M., Korićanac Goran, Tepavčević Snežana, Ćulafić Tijana, Romić Snježana Đ., Stanišić Jelena, Pantelić Marija, Stojiljković Mirjana, "Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet" 8, no. Supplement 1 (2018):223,
https://doi.org/10.1002/2211-5463.12453 .
18
10

Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise

Stanišić, Jelena; Korićanac, Goran; Stojiljković, Mojca D.; Ćulafić, Tijana; Romić, Snježana Đ.; Kostić, Mirjana M.; Pantelić, M.; Tepavčević, Snežana

(2018)

TY  - CONF
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Stojiljković, Mojca D.
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Kostić, Mirjana M.
AU  - Pantelić, M.
AU  - Tepavčević, Snežana
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0021915018309195
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7945
C3  - Atherosclerosis
T1  - Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise
VL  - 275
SP  - e197
DO  - 10.1016/j.atherosclerosis.2018.06.607
ER  - 
@conference{
author = "Stanišić, Jelena and Korićanac, Goran and Stojiljković, Mojca D. and Ćulafić, Tijana and Romić, Snježana Đ. and Kostić, Mirjana M. and Pantelić, M. and Tepavčević, Snežana",
year = "2018",
url = "https://linkinghub.elsevier.com/retrieve/pii/S0021915018309195, http://vinar.vin.bg.ac.rs/handle/123456789/7945",
journal = "Atherosclerosis",
title = "Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise",
volume = "275",
pages = "e197",
doi = "10.1016/j.atherosclerosis.2018.06.607"
}
Stanišić, J., Korićanac, G., Stojiljković, M. D., Ćulafić, T., Romić, S. Đ., Kostić, M. M., Pantelić, M.,& Tepavčević, S. (2018). Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise.
Atherosclerosis, 275, e197.
https://doi.org/10.1016/j.atherosclerosis.2018.06.607
Stanišić J, Korićanac G, Stojiljković MD, Ćulafić T, Romić SĐ, Kostić MM, Pantelić M, Tepavčević S. Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise. Atherosclerosis. 2018;275:e197
Stanišić Jelena, Korićanac Goran, Stojiljković Mojca D., Ćulafić Tijana, Romić Snježana Đ., Kostić Mirjana M., Pantelić M., Tepavčević Snežana, "Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise" 275 (2018):e197,
https://doi.org/10.1016/j.atherosclerosis.2018.06.607 .

Involvement of Atp-Sensitive Potassium Channels in Effect of Pinacidil on Saphenous Vein Obtained from the Patients with and Without Type 2 Diabetes Mellitus

Rajković, Jovana; Perić, Miodrag; Stanišić, Jelena; Rakočević, Jelena T.; Novaković, Radmila; Đokić, Vladimir; Labudović-Borović, Milica; Tepavčević, Snežana; Zivanovic, Vladimir; Kanjuh, Vladimir; Heinle, Helmut; Gojković-Bukarica, Ljiljana

(2017)

TY  - CONF
AU  - Rajković, Jovana
AU  - Perić, Miodrag
AU  - Stanišić, Jelena
AU  - Rakočević, Jelena T.
AU  - Novaković, Radmila
AU  - Đokić, Vladimir
AU  - Labudović-Borović, Milica
AU  - Tepavčević, Snežana
AU  - Zivanovic, Vladimir
AU  - Kanjuh, Vladimir
AU  - Heinle, Helmut
AU  - Gojković-Bukarica, Ljiljana
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7174
C3  - Atherosclerosis
T1  - Involvement of Atp-Sensitive Potassium Channels in Effect of Pinacidil on Saphenous Vein Obtained from the Patients with and Without Type 2 Diabetes Mellitus
VL  - 263
SP  - E131
EP  - E131
DO  - 10.1016/j.atherosclerosis.2017.06.418
ER  - 
@conference{
author = "Rajković, Jovana and Perić, Miodrag and Stanišić, Jelena and Rakočević, Jelena T. and Novaković, Radmila and Đokić, Vladimir and Labudović-Borović, Milica and Tepavčević, Snežana and Zivanovic, Vladimir and Kanjuh, Vladimir and Heinle, Helmut and Gojković-Bukarica, Ljiljana",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7174",
journal = "Atherosclerosis",
title = "Involvement of Atp-Sensitive Potassium Channels in Effect of Pinacidil on Saphenous Vein Obtained from the Patients with and Without Type 2 Diabetes Mellitus",
volume = "263",
pages = "E131-E131",
doi = "10.1016/j.atherosclerosis.2017.06.418"
}
Rajković, J., Perić, M., Stanišić, J., Rakočević, J. T., Novaković, R., Đokić, V., Labudović-Borović, M., Tepavčević, S., Zivanovic, V., Kanjuh, V., Heinle, H.,& Gojković-Bukarica, L. (2017). Involvement of Atp-Sensitive Potassium Channels in Effect of Pinacidil on Saphenous Vein Obtained from the Patients with and Without Type 2 Diabetes Mellitus.
Atherosclerosis, 263, E131-E131.
https://doi.org/10.1016/j.atherosclerosis.2017.06.418
Rajković J, Perić M, Stanišić J, Rakočević JT, Novaković R, Đokić V, Labudović-Borović M, Tepavčević S, Zivanovic V, Kanjuh V, Heinle H, Gojković-Bukarica L. Involvement of Atp-Sensitive Potassium Channels in Effect of Pinacidil on Saphenous Vein Obtained from the Patients with and Without Type 2 Diabetes Mellitus. Atherosclerosis. 2017;263:E131-E131
Rajković Jovana, Perić Miodrag, Stanišić Jelena, Rakočević Jelena T., Novaković Radmila, Đokić Vladimir, Labudović-Borović Milica, Tepavčević Snežana, Zivanovic Vladimir, Kanjuh Vladimir, Heinle Helmut, Gojković-Bukarica Ljiljana, "Involvement of Atp-Sensitive Potassium Channels in Effect of Pinacidil on Saphenous Vein Obtained from the Patients with and Without Type 2 Diabetes Mellitus" 263 (2017):E131-E131,
https://doi.org/10.1016/j.atherosclerosis.2017.06.418 .

Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet

Stanišić, Jelena; Korićanac, Goran; Ćulafić, Tijana; Romić, Snježana Đ.; Stojiljković, Mojca D.; Kostić, Milan; Pantelić, Marija; Tepavčević, Snežana

(2016)

TY  - JOUR
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Pantelić, Marija
AU  - Tepavčević, Snežana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/913
AB  - Increase in fructose consumption together with decrease in physical activity contributes to the development of metabolic syndrome and consequently cardiovascular diseases. The current study examined the preventive role of exercise on defects in cardiac insulin signaling and function of endothelial nitric oxide synthase (eNOS) in fructose fed rats. Male Wistar rats were divided into control, sedentary fructose (received 10% fructose for 9 weeks) and exercise fructose (additionally exposed to low intensity exercise) groups. Concentration of triglycerides, glucose, insulin and visceral adipose tissue weight were determined to estimate metabolic syndrome development. Expression and/or phosphorylation of cardiac insulin receptor (IR), insulin receptor substrate 1 (IRS1), tyrosine-specific protein phosphatase 1B (PTP1B), Akt, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and eNOS were evaluated. Fructose overload increased visceral adipose tissue, insulin concentration and homeostasis model assessment index. Exercise managed to decrease visceral adiposity and insulin level and to increase insulin sensitivity. Fructose diet increased level of cardiac PTP1B and pIRS1 (Ser307), while levels of IR and ERK1/2, as well as pIRS1 (Tyr 632), pAkt (Ser473, Thr308) and pERK1/2 were decreased. These disturbances were accompanied by reduced phosphorylation of eNOS at Ser1177. Exercise managed to prevent most of the disturbances in insulin signaling caused by fructose diet (except phosphorylation of IRS1 at Tyr 632 and phosphorylation and protein expression of ERK1/2) and consequently restored function of eNOS. Low intensity exercise could be considered as efficient treatment of cardiac insulin resistance induced by fructose diet. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
T2  - Molecular and Cellular Endocrinology
T1  - Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet
VL  - 420
IS  - C
SP  - 97
EP  - 104
DO  - 10.1016/j.mce.2015.11.032
ER  - 
@article{
author = "Stanišić, Jelena and Korićanac, Goran and Ćulafić, Tijana and Romić, Snježana Đ. and Stojiljković, Mojca D. and Kostić, Milan and Pantelić, Marija and Tepavčević, Snežana",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/913",
abstract = "Increase in fructose consumption together with decrease in physical activity contributes to the development of metabolic syndrome and consequently cardiovascular diseases. The current study examined the preventive role of exercise on defects in cardiac insulin signaling and function of endothelial nitric oxide synthase (eNOS) in fructose fed rats. Male Wistar rats were divided into control, sedentary fructose (received 10% fructose for 9 weeks) and exercise fructose (additionally exposed to low intensity exercise) groups. Concentration of triglycerides, glucose, insulin and visceral adipose tissue weight were determined to estimate metabolic syndrome development. Expression and/or phosphorylation of cardiac insulin receptor (IR), insulin receptor substrate 1 (IRS1), tyrosine-specific protein phosphatase 1B (PTP1B), Akt, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and eNOS were evaluated. Fructose overload increased visceral adipose tissue, insulin concentration and homeostasis model assessment index. Exercise managed to decrease visceral adiposity and insulin level and to increase insulin sensitivity. Fructose diet increased level of cardiac PTP1B and pIRS1 (Ser307), while levels of IR and ERK1/2, as well as pIRS1 (Tyr 632), pAkt (Ser473, Thr308) and pERK1/2 were decreased. These disturbances were accompanied by reduced phosphorylation of eNOS at Ser1177. Exercise managed to prevent most of the disturbances in insulin signaling caused by fructose diet (except phosphorylation of IRS1 at Tyr 632 and phosphorylation and protein expression of ERK1/2) and consequently restored function of eNOS. Low intensity exercise could be considered as efficient treatment of cardiac insulin resistance induced by fructose diet. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
journal = "Molecular and Cellular Endocrinology",
title = "Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet",
volume = "420",
number = "C",
pages = "97-104",
doi = "10.1016/j.mce.2015.11.032"
}
Stanišić, J., Korićanac, G., Ćulafić, T., Romić, S. Đ., Stojiljković, M. D., Kostić, M., Pantelić, M.,& Tepavčević, S. (2016). Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet.
Molecular and Cellular Endocrinology, 420(C), 97-104.
https://doi.org/10.1016/j.mce.2015.11.032
Stanišić J, Korićanac G, Ćulafić T, Romić SĐ, Stojiljković MD, Kostić M, Pantelić M, Tepavčević S. Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet. Molecular and Cellular Endocrinology. 2016;420(C):97-104
Stanišić Jelena, Korićanac Goran, Ćulafić Tijana, Romić Snježana Đ., Stojiljković Mojca D., Kostić Milan, Pantelić Marija, Tepavčević Snežana, "Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet" 420, no. C (2016):97-104,
https://doi.org/10.1016/j.mce.2015.11.032 .
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Cardiac Nitric Oxide Synthases and Na+/K+-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosterone

Tepavčević, Snežana; Milutinovic, D. V.; Macut, D.; Stanišić, Jelena; Nikolic, M.; Bozic-Antic, I.; Rodaljevic, S.; Bjekic-Macut, J.; Matić, Gordana; Korićanac, Goran

(2015)

TY  - JOUR
AU  - Tepavčević, Snežana
AU  - Milutinovic, D. V.
AU  - Macut, D.
AU  - Stanišić, Jelena
AU  - Nikolic, M.
AU  - Bozic-Antic, I.
AU  - Rodaljevic, S.
AU  - Bjekic-Macut, J.
AU  - Matić, Gordana
AU  - Korićanac, Goran
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/553
AB  - Nitric oxide synthases (NOSs) and Na+/K+-ATPase are enzymes essential for regular functioning of the heart. Since both enzymes are under insulin and androgen regulation and since insulin action and androgen level were disturbed in polycystic ovary syndrome (PCOS), we hypothesized that cardiac nitric oxide (NO) production and sodium/potassium transport would be deteriorated in PCOS. To test our hypothesis we introduced animal model of PCOS based on dihydrotestosterone (DHT) treatment of female Wistar rats and analyzed protein expression, phosphorylation or subcellular localization of endothelial NOS (eNOS), inducible NOS (iNOS) and alpha subunits of Na+/K+-ATPase in the heart. Obtained results indicate that DHT treatment significantly decreased cardiac eNOS protein level and activating phosphorylation at serine 1177, while inhibitory phosphorylation at threonine 495 was increased. In contrast to expression of eNOS, iNOS protein level in the heart of DHT-treated rats was significantly elevated. Furthermore, cardiac protein level of alpha 1 subunit of the ATPase, as well as its plasma membrane content, were decreased in rats with PCOS. In line with this, alpha 2 subunit protein level in fraction of plasma membranes was also significantly below control level. In conclusion, DHT treatment impaired effectiveness of NOSs and Na+/K+-ATPase in the female rat heart. Regarding the importance of NO production and sodium/potassium transport in the cardiac contraction and blood flow regulation, it implicates strong consequences of PCOS for heart functioning.
T2  - Experimental and Clinical Endocrinology and Diabetes
T1  - Cardiac Nitric Oxide Synthases and Na+/K+-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosterone
VL  - 123
IS  - 5
SP  - 303
EP  - 307
DO  - 10.1055/s-0035-1548929
ER  - 
@article{
author = "Tepavčević, Snežana and Milutinovic, D. V. and Macut, D. and Stanišić, Jelena and Nikolic, M. and Bozic-Antic, I. and Rodaljevic, S. and Bjekic-Macut, J. and Matić, Gordana and Korićanac, Goran",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/553",
abstract = "Nitric oxide synthases (NOSs) and Na+/K+-ATPase are enzymes essential for regular functioning of the heart. Since both enzymes are under insulin and androgen regulation and since insulin action and androgen level were disturbed in polycystic ovary syndrome (PCOS), we hypothesized that cardiac nitric oxide (NO) production and sodium/potassium transport would be deteriorated in PCOS. To test our hypothesis we introduced animal model of PCOS based on dihydrotestosterone (DHT) treatment of female Wistar rats and analyzed protein expression, phosphorylation or subcellular localization of endothelial NOS (eNOS), inducible NOS (iNOS) and alpha subunits of Na+/K+-ATPase in the heart. Obtained results indicate that DHT treatment significantly decreased cardiac eNOS protein level and activating phosphorylation at serine 1177, while inhibitory phosphorylation at threonine 495 was increased. In contrast to expression of eNOS, iNOS protein level in the heart of DHT-treated rats was significantly elevated. Furthermore, cardiac protein level of alpha 1 subunit of the ATPase, as well as its plasma membrane content, were decreased in rats with PCOS. In line with this, alpha 2 subunit protein level in fraction of plasma membranes was also significantly below control level. In conclusion, DHT treatment impaired effectiveness of NOSs and Na+/K+-ATPase in the female rat heart. Regarding the importance of NO production and sodium/potassium transport in the cardiac contraction and blood flow regulation, it implicates strong consequences of PCOS for heart functioning.",
journal = "Experimental and Clinical Endocrinology and Diabetes",
title = "Cardiac Nitric Oxide Synthases and Na+/K+-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosterone",
volume = "123",
number = "5",
pages = "303-307",
doi = "10.1055/s-0035-1548929"
}
Tepavčević, S., Milutinovic, D. V., Macut, D., Stanišić, J., Nikolic, M., Bozic-Antic, I., Rodaljevic, S., Bjekic-Macut, J., Matić, G.,& Korićanac, G. (2015). Cardiac Nitric Oxide Synthases and Na+/K+-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosterone.
Experimental and Clinical Endocrinology and Diabetes, 123(5), 303-307.
https://doi.org/10.1055/s-0035-1548929
Tepavčević S, Milutinovic DV, Macut D, Stanišić J, Nikolic M, Bozic-Antic I, Rodaljevic S, Bjekic-Macut J, Matić G, Korićanac G. Cardiac Nitric Oxide Synthases and Na+/K+-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosterone. Experimental and Clinical Endocrinology and Diabetes. 2015;123(5):303-307
Tepavčević Snežana, Milutinovic D. V., Macut D., Stanišić Jelena, Nikolic M., Bozic-Antic I., Rodaljevic S., Bjekic-Macut J., Matić Gordana, Korićanac Goran, "Cardiac Nitric Oxide Synthases and Na+/K+-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosterone" 123, no. 5 (2015):303-307,
https://doi.org/10.1055/s-0035-1548929 .
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