TY  - JOUR
AU  - Senćanski, Milan
AU  - Perović, Vladimir
AU  - Milićević, Jelena S.
AU  - Todorović, Tamara
AU  - Prodanović, Radivoje
AU  - Veljković, Veljko
AU  - Paessler, Slobodan
AU  - Glišić, Sanja
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10425
AB  - In the currentpandemic,findingan effectivedrugto preventortreatthe infectionis the highestpriority.A rapidand safeapproachto counteractCOVID-19is in silicodrugrepurposing.The SARS-CoV-2PLpropromotesviral replicationand modu-latesthe hostimmunesystem,resultingin inhibitionof thehostantiviralinnateimmuneresponse,and thereforeis anattractivedrugtarget.In this study,we useda combinedinsilicovirtualscreeningfor candidatesfor SARS-CoV-2PLproproteaseinhibitors.We usedthe Informationalspectrummethodappliedfor SmallMoleculesfor searchingthe Drugbankdatabasefollowedby moleculardocking.Afterin silicoscreen-ing of drugspace,we identified44 drugsas potentialSARS-CoV-2PLproinhibitorsthat we proposefor furtherexperimentaltesting.
T2  - ChemistryOpen
T1  - Identification of SARS-CoV-2 Papain-like Protease (PLpro) Inhibitors Using Combined Computational Approach
VL  - 11
IS  - 2
SP  - e202100248
DO  - 10.1002/open.202100248
ER  - 

@article{
author = "Senćanski, Milan and Perović, Vladimir and Milićević, Jelena S. and Todorović, Tamara and Prodanović, Radivoje and Veljković, Veljko and Paessler, Slobodan and Glišić, Sanja",
year = "2022",
abstract = "In the currentpandemic,findingan effectivedrugto preventortreatthe infectionis the highestpriority.A rapidand safeapproachto counteractCOVID-19is in silicodrugrepurposing.The SARS-CoV-2PLpropromotesviral replicationand modu-latesthe hostimmunesystem,resultingin inhibitionof thehostantiviralinnateimmuneresponse,and thereforeis anattractivedrugtarget.In this study,we useda combinedinsilicovirtualscreeningfor candidatesfor SARS-CoV-2PLproproteaseinhibitors.We usedthe Informationalspectrummethodappliedfor SmallMoleculesfor searchingthe Drugbankdatabasefollowedby moleculardocking.Afterin silicoscreen-ing of drugspace,we identified44 drugsas potentialSARS-CoV-2PLproinhibitorsthat we proposefor furtherexperimentaltesting.",
journal = "ChemistryOpen",
title = "Identification of SARS-CoV-2 Papain-like Protease (PLpro) Inhibitors Using Combined Computational Approach",
volume = "11",
number = "2",
pages = "e202100248",
doi = "10.1002/open.202100248"
}

Senćanski, M., Perović, V., Milićević, J. S., Todorović, T., Prodanović, R., Veljković, V., Paessler, S.,& Glišić, S.. (2022). Identification of SARS-CoV-2 Papain-like Protease (PLpro) Inhibitors Using Combined Computational Approach. in ChemistryOpen, 11(2), e202100248.
https://doi.org/10.1002/open.202100248

Senćanski M, Perović V, Milićević JS, Todorović T, Prodanović R, Veljković V, Paessler S, Glišić S. Identification of SARS-CoV-2 Papain-like Protease (PLpro) Inhibitors Using Combined Computational Approach. in ChemistryOpen. 2022;11(2):e202100248.
doi:10.1002/open.202100248 .

Senćanski, Milan, Perović, Vladimir, Milićević, Jelena S., Todorović, Tamara, Prodanović, Radivoje, Veljković, Veljko, Paessler, Slobodan, Glišić, Sanja, "Identification of SARS-CoV-2 Papain-like Protease (PLpro) Inhibitors Using Combined Computational Approach" in ChemistryOpen, 11, no. 2 (2022):e202100248,
https://doi.org/10.1002/open.202100248 . .

TY  - JOUR
AU  - Matejin, Stanislava
AU  - Bukreyeva, Natalya
AU  - Radošević, Draginja
AU  - Senćanski, Milan V.
AU  - Mantlo, Emily
AU  - Veljković, Veljko
AU  - Glišić, Sanja
AU  - Paessler, Slobodan
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9036
AB  - Background: Due to the limitations of current antiviral therapies because of drug resistance and the emergence of new circulating viral strains, novel effective antivirals are urgently needed. Results of the previous drug repurposing by virtual screening of DrugBank revealed the anticholinergic drug cycrimine as a possible inhibitor of the influenza virus infection. Methods: In this study we examined the potential antiviral activity of cycrimine in vitro. Results: The experimental results showed the anti-influenza activity of cycrimine against two different influenza A subtypes in cell culture. Conclusions: The findings of this study suggest cycrimine as a potential therapeutic agent for influenza. ©2019 International Medical Press.
T2  - Antiviral Therapy
T1  - In vitro anti-influenza activity of in silico repurposed candidate drug Cycrimine
VL  - 24
IS  - 8
SP  - 589
EP  - 593
DO  - 10.3851/IMP3348
ER  - 

@article{
author = "Matejin, Stanislava and Bukreyeva, Natalya and Radošević, Draginja and Senćanski, Milan V. and Mantlo, Emily and Veljković, Veljko and Glišić, Sanja and Paessler, Slobodan",
year = "2020",
abstract = "Background: Due to the limitations of current antiviral therapies because of drug resistance and the emergence of new circulating viral strains, novel effective antivirals are urgently needed. Results of the previous drug repurposing by virtual screening of DrugBank revealed the anticholinergic drug cycrimine as a possible inhibitor of the influenza virus infection. Methods: In this study we examined the potential antiviral activity of cycrimine in vitro. Results: The experimental results showed the anti-influenza activity of cycrimine against two different influenza A subtypes in cell culture. Conclusions: The findings of this study suggest cycrimine as a potential therapeutic agent for influenza. ©2019 International Medical Press.",
journal = "Antiviral Therapy",
title = "In vitro anti-influenza activity of in silico repurposed candidate drug Cycrimine",
volume = "24",
number = "8",
pages = "589-593",
doi = "10.3851/IMP3348"
}

Matejin, S., Bukreyeva, N., Radošević, D., Senćanski, M. V., Mantlo, E., Veljković, V., Glišić, S.,& Paessler, S.. (2020). In vitro anti-influenza activity of in silico repurposed candidate drug Cycrimine. in Antiviral Therapy, 24(8), 589-593.
https://doi.org/10.3851/IMP3348

Matejin S, Bukreyeva N, Radošević D, Senćanski MV, Mantlo E, Veljković V, Glišić S, Paessler S. In vitro anti-influenza activity of in silico repurposed candidate drug Cycrimine. in Antiviral Therapy. 2020;24(8):589-593.
doi:10.3851/IMP3348 .

Matejin, Stanislava, Bukreyeva, Natalya, Radošević, Draginja, Senćanski, Milan V., Mantlo, Emily, Veljković, Veljko, Glišić, Sanja, Paessler, Slobodan, "In vitro anti-influenza activity of in silico repurposed candidate drug Cycrimine" in Antiviral Therapy, 24, no. 8 (2020):589-593,
https://doi.org/10.3851/IMP3348 . .

TY  - JOUR
AU  - Glišić, Sanja
AU  - Perović, Vladimir R.
AU  - Senćanski, Milan V.
AU  - Paessler, Slobodan
AU  - Veljković, Veljko
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9723
AB  - The Bacillus Calmette–Guerin vaccine is still widely used in the developing world. The vaccination prevents infant death not only from tuberculosis but also from unrelated infectious agents, especially respiratory tract infections and neonatal sepsis. It is proposed that these off-target protective effects of the BCG vaccine are mediated by the general long-term boosting of innate immune mechanisms, also termed “trained innate immunity”. Recent studies indicate that both COVID-19 incidence and total deaths are strongly associated with the presence or absence of national mandatory BCG vaccination programs and encourage the initiation of several clinical studies with the expectation that revaccination with BCG could reduce the incidence and severity of COVID-19. Here, presented results from the bioinformatics analysis of the Mycobacterium bovis (strain BCG/Pasteur 1173P2) proteome suggests four immunodominant antigens that could induce an immune response against SARS-CoV-2.
T2  - Journal of Proteome Research
T2  - Journal of Proteome ResearchJ. Proteome Res.
T1  - Biological Rationale for the Repurposing of BCG Vaccine against SARS-CoV-2
VL  - 19
IS  - 11
SP  - 4649
EP  - 4654
DO  - 10.1021/acs.jproteome.0c00410
ER  - 

@article{
author = "Glišić, Sanja and Perović, Vladimir R. and Senćanski, Milan V. and Paessler, Slobodan and Veljković, Veljko",
year = "2020",
abstract = "The Bacillus Calmette–Guerin vaccine is still widely used in the developing world. The vaccination prevents infant death not only from tuberculosis but also from unrelated infectious agents, especially respiratory tract infections and neonatal sepsis. It is proposed that these off-target protective effects of the BCG vaccine are mediated by the general long-term boosting of innate immune mechanisms, also termed “trained innate immunity”. Recent studies indicate that both COVID-19 incidence and total deaths are strongly associated with the presence or absence of national mandatory BCG vaccination programs and encourage the initiation of several clinical studies with the expectation that revaccination with BCG could reduce the incidence and severity of COVID-19. Here, presented results from the bioinformatics analysis of the Mycobacterium bovis (strain BCG/Pasteur 1173P2) proteome suggests four immunodominant antigens that could induce an immune response against SARS-CoV-2.",
journal = "Journal of Proteome Research, Journal of Proteome ResearchJ. Proteome Res.",
title = "Biological Rationale for the Repurposing of BCG Vaccine against SARS-CoV-2",
volume = "19",
number = "11",
pages = "4649-4654",
doi = "10.1021/acs.jproteome.0c00410"
}

Glišić, S., Perović, V. R., Senćanski, M. V., Paessler, S.,& Veljković, V.. (2020). Biological Rationale for the Repurposing of BCG Vaccine against SARS-CoV-2. in Journal of Proteome Research, 19(11), 4649-4654.
https://doi.org/10.1021/acs.jproteome.0c00410

Glišić S, Perović VR, Senćanski MV, Paessler S, Veljković V. Biological Rationale for the Repurposing of BCG Vaccine against SARS-CoV-2. in Journal of Proteome Research. 2020;19(11):4649-4654.
doi:10.1021/acs.jproteome.0c00410 .

Glišić, Sanja, Perović, Vladimir R., Senćanski, Milan V., Paessler, Slobodan, Veljković, Veljko, "Biological Rationale for the Repurposing of BCG Vaccine against SARS-CoV-2" in Journal of Proteome Research, 19, no. 11 (2020):4649-4654,
https://doi.org/10.1021/acs.jproteome.0c00410 . .

TY  - JOUR
AU  - Radošević, Draginja
AU  - Senćanski, Milan V.
AU  - Perović, Vladimir R.
AU  - Veljković, Nevena V.
AU  - Prljić, Jelena
AU  - Veljković, Veljko
AU  - Mantlo, Emily
AU  - Bukreyeva, Natalya
AU  - Paessler, Slobodan
AU  - Glišić, Sanja
PY  - 2019
UR  - https://www.frontiersin.org/article/10.3389/fcimb.2019.00067/full
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8163
AB  - Influenza A virus (IAV) matrix protein 2 (M2), an ion channel, is crucial for virus infection, and therefore, an important anti-influenza drug target. Adamantanes, also known as M2 channel blockers, are one of the two classes of Food and Drug Administration-approved anti-influenza drugs, although their use was discontinued due to prevalent drug resistance. Fast emergence of resistance to current anti-influenza drugs have raised an urgent need for developing new anti-influenza drugs against resistant forms of circulating viruses. Here we propose a simple theoretical criterion for fast virtual screening of molecular libraries for candidate anti-influenza ion channel inhibitors both for wild type and adamantane-resistant influenza A viruses. After in silico screening of drug space using the EIIP/AQVN filter and further filtering of drugs by ligand based virtual screening and molecular docking we propose the best candidate drugs as potential dual inhibitors of wild type and adamantane-resistant influenza A viruses. Finally, guanethidine, the best ranked drug selected from ligand-based virtual screening, was experimentally tested. The experimental results show measurable anti-influenza activity of guanethidine in cell culture. © 2019 Radosevic, Sencanski, Perovic, Veljkovic, Prljic, Veljkovic, Mantlo, Bukreyeva, Paessler and Glisic.
T2  - Frontiers in Cellular and Infection Microbiology
T1  - Virtual Screen for Repurposing of Drugs for Candidate Influenza a M2 Ion-Channel Inhibitors
VL  - 9
SP  - 67
DO  - 10.3389/fcimb.2019.00067
ER  - 

@article{
author = "Radošević, Draginja and Senćanski, Milan V. and Perović, Vladimir R. and Veljković, Nevena V. and Prljić, Jelena and Veljković, Veljko and Mantlo, Emily and Bukreyeva, Natalya and Paessler, Slobodan and Glišić, Sanja",
year = "2019",
abstract = "Influenza A virus (IAV) matrix protein 2 (M2), an ion channel, is crucial for virus infection, and therefore, an important anti-influenza drug target. Adamantanes, also known as M2 channel blockers, are one of the two classes of Food and Drug Administration-approved anti-influenza drugs, although their use was discontinued due to prevalent drug resistance. Fast emergence of resistance to current anti-influenza drugs have raised an urgent need for developing new anti-influenza drugs against resistant forms of circulating viruses. Here we propose a simple theoretical criterion for fast virtual screening of molecular libraries for candidate anti-influenza ion channel inhibitors both for wild type and adamantane-resistant influenza A viruses. After in silico screening of drug space using the EIIP/AQVN filter and further filtering of drugs by ligand based virtual screening and molecular docking we propose the best candidate drugs as potential dual inhibitors of wild type and adamantane-resistant influenza A viruses. Finally, guanethidine, the best ranked drug selected from ligand-based virtual screening, was experimentally tested. The experimental results show measurable anti-influenza activity of guanethidine in cell culture. © 2019 Radosevic, Sencanski, Perovic, Veljkovic, Prljic, Veljkovic, Mantlo, Bukreyeva, Paessler and Glisic.",
journal = "Frontiers in Cellular and Infection Microbiology",
title = "Virtual Screen for Repurposing of Drugs for Candidate Influenza a M2 Ion-Channel Inhibitors",
volume = "9",
pages = "67",
doi = "10.3389/fcimb.2019.00067"
}

Radošević, D., Senćanski, M. V., Perović, V. R., Veljković, N. V., Prljić, J., Veljković, V., Mantlo, E., Bukreyeva, N., Paessler, S.,& Glišić, S.. (2019). Virtual Screen for Repurposing of Drugs for Candidate Influenza a M2 Ion-Channel Inhibitors. in Frontiers in Cellular and Infection Microbiology, 9, 67.
https://doi.org/10.3389/fcimb.2019.00067

Radošević D, Senćanski MV, Perović VR, Veljković NV, Prljić J, Veljković V, Mantlo E, Bukreyeva N, Paessler S, Glišić S. Virtual Screen for Repurposing of Drugs for Candidate Influenza a M2 Ion-Channel Inhibitors. in Frontiers in Cellular and Infection Microbiology. 2019;9:67.
doi:10.3389/fcimb.2019.00067 .

Radošević, Draginja, Senćanski, Milan V., Perović, Vladimir R., Veljković, Nevena V., Prljić, Jelena, Veljković, Veljko, Mantlo, Emily, Bukreyeva, Natalya, Paessler, Slobodan, Glišić, Sanja, "Virtual Screen for Repurposing of Drugs for Candidate Influenza a M2 Ion-Channel Inhibitors" in Frontiers in Cellular and Infection Microbiology, 9 (2019):67,
https://doi.org/10.3389/fcimb.2019.00067 . .

TY  - JOUR
AU  - Sistere-Oro, Marta
AU  - Vergara-Alert, Julia
AU  - Stratmann, Thomas
AU  - Lopez-Serrano, Sergi
AU  - Pina-Pedrero, Sonia
AU  - Cordoba, Lorena
AU  - Perez-Maillo, Monica
AU  - Pleguezuelos, Patricia
AU  - Vidal, Enric
AU  - Veljković, Veljko
AU  - Segalés, Joaquim
AU  - Nielsen, Jens Peter
AU  - Fomsgaard, Anders
AU  - Darji, Ayub
AU  - Huber, Victor C.
PY  - 2019
UR  - http://dx.plos.org/10.1371/journal.pone.0212431
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8083
AB  - Swine influenza viruses (SIVs), the causal agents of swine influenza, are not only important to control due to the economic losses in the swine industry, but also can be pandemic pathogens. Vaccination is one of the most relevant strategies to control and prevent influenza infection. Current human vaccines against influenza induce strain-specific immunity and annual update is required due to the virus antigenic shift phenomena. Previously, our group has reported the use of conserved hemagglutinin peptides (HA-peptides) derived from H1-influenza virus as a potential multivalent vaccine candidate. Immunization of swine with these HA-peptides elicited antibodies that recognized and neutralized heterologous influenza viruses in vitro and demonstrated strong hemagglutination-inhibiting activity. In the present work, we cloned one HA-peptide (named NG34) into a plasmid fused with cytotoxic T lymphocyte-associated antigen (CTLA4) which is a molecule that modifies T cell activation and with an adjuvant activity interfering with the adaptive immune response. The resulting plasmid, named pCMV-CTLA4-Ig-NG34, was administered twice to animals employing a needle-free delivery approach. Two studies were carried out to test the efficacy of pCMV-CTLA4-Ig-NG34 as a potential swine influenza vaccine, one in seronegative and another in seropositive pigs against SIV. The second one was aimed to evaluate whether pCMV-CTLA4-Ig-NG34 vaccination would overcome maternally derived antibodies (MDA). After immunization, all animals were intranasally challenged with an H3N2 influenza strain. A complete elimination or significant reduction in the viral shedding was observed within the first week after the challenge in the vaccinated animals from both studies. In addition, no challenged heterologous virus load was detected in the airways of vaccinated pigs. Overall, it is suggested that the pCMV-CTLA4-Ig-NG34 vaccine formulation could potentially be used as a multivalent vaccine against influenza viruses. © 2019 Sisteré-Oró et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
T2  - PLOS One
T1  - Conserved HA-peptide NG34 formulated in pCMV-CTLA4-Ig reduces viral shedding in pigs after a heterosubtypic influenza virus SwH3N2 challenge
VL  - 14
IS  - 3
SP  - e0212431
DO  - 10.1371/journal.pone.0212431
ER  - 

@article{
author = "Sistere-Oro, Marta and Vergara-Alert, Julia and Stratmann, Thomas and Lopez-Serrano, Sergi and Pina-Pedrero, Sonia and Cordoba, Lorena and Perez-Maillo, Monica and Pleguezuelos, Patricia and Vidal, Enric and Veljković, Veljko and Segalés, Joaquim and Nielsen, Jens Peter and Fomsgaard, Anders and Darji, Ayub and Huber, Victor C.",
year = "2019",
abstract = "Swine influenza viruses (SIVs), the causal agents of swine influenza, are not only important to control due to the economic losses in the swine industry, but also can be pandemic pathogens. Vaccination is one of the most relevant strategies to control and prevent influenza infection. Current human vaccines against influenza induce strain-specific immunity and annual update is required due to the virus antigenic shift phenomena. Previously, our group has reported the use of conserved hemagglutinin peptides (HA-peptides) derived from H1-influenza virus as a potential multivalent vaccine candidate. Immunization of swine with these HA-peptides elicited antibodies that recognized and neutralized heterologous influenza viruses in vitro and demonstrated strong hemagglutination-inhibiting activity. In the present work, we cloned one HA-peptide (named NG34) into a plasmid fused with cytotoxic T lymphocyte-associated antigen (CTLA4) which is a molecule that modifies T cell activation and with an adjuvant activity interfering with the adaptive immune response. The resulting plasmid, named pCMV-CTLA4-Ig-NG34, was administered twice to animals employing a needle-free delivery approach. Two studies were carried out to test the efficacy of pCMV-CTLA4-Ig-NG34 as a potential swine influenza vaccine, one in seronegative and another in seropositive pigs against SIV. The second one was aimed to evaluate whether pCMV-CTLA4-Ig-NG34 vaccination would overcome maternally derived antibodies (MDA). After immunization, all animals were intranasally challenged with an H3N2 influenza strain. A complete elimination or significant reduction in the viral shedding was observed within the first week after the challenge in the vaccinated animals from both studies. In addition, no challenged heterologous virus load was detected in the airways of vaccinated pigs. Overall, it is suggested that the pCMV-CTLA4-Ig-NG34 vaccine formulation could potentially be used as a multivalent vaccine against influenza viruses. © 2019 Sisteré-Oró et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
journal = "PLOS One",
title = "Conserved HA-peptide NG34 formulated in pCMV-CTLA4-Ig reduces viral shedding in pigs after a heterosubtypic influenza virus SwH3N2 challenge",
volume = "14",
number = "3",
pages = "e0212431",
doi = "10.1371/journal.pone.0212431"
}

Sistere-Oro, M., Vergara-Alert, J., Stratmann, T., Lopez-Serrano, S., Pina-Pedrero, S., Cordoba, L., Perez-Maillo, M., Pleguezuelos, P., Vidal, E., Veljković, V., Segalés, J., Nielsen, J. P., Fomsgaard, A., Darji, A.,& Huber, V. C.. (2019). Conserved HA-peptide NG34 formulated in pCMV-CTLA4-Ig reduces viral shedding in pigs after a heterosubtypic influenza virus SwH3N2 challenge. in PLOS One, 14(3), e0212431.
https://doi.org/10.1371/journal.pone.0212431

Sistere-Oro M, Vergara-Alert J, Stratmann T, Lopez-Serrano S, Pina-Pedrero S, Cordoba L, Perez-Maillo M, Pleguezuelos P, Vidal E, Veljković V, Segalés J, Nielsen JP, Fomsgaard A, Darji A, Huber VC. Conserved HA-peptide NG34 formulated in pCMV-CTLA4-Ig reduces viral shedding in pigs after a heterosubtypic influenza virus SwH3N2 challenge. in PLOS One. 2019;14(3):e0212431.
doi:10.1371/journal.pone.0212431 .

Sistere-Oro, Marta, Vergara-Alert, Julia, Stratmann, Thomas, Lopez-Serrano, Sergi, Pina-Pedrero, Sonia, Cordoba, Lorena, Perez-Maillo, Monica, Pleguezuelos, Patricia, Vidal, Enric, Veljković, Veljko, Segalés, Joaquim, Nielsen, Jens Peter, Fomsgaard, Anders, Darji, Ayub, Huber, Victor C., "Conserved HA-peptide NG34 formulated in pCMV-CTLA4-Ig reduces viral shedding in pigs after a heterosubtypic influenza virus SwH3N2 challenge" in PLOS One, 14, no. 3 (2019):e0212431,
https://doi.org/10.1371/journal.pone.0212431 . .

TY  - JOUR
AU  - Paessler, Slobodan
AU  - Huang, Cheng
AU  - Senćanski, Milan V.
AU  - Veljković, Nevena V.
AU  - Perović, Vladimir R.
AU  - Glišić, Sanja
AU  - Veljković, Veljko
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7695
AB  - The Ebola virus outbreak in West Africa 2015 and Congo 2017, point out an urgent need for development of drugs against this important pathogen. Previously, by repurposing virtual screening of 6438 drugs from DrugBank, ibuprofen was selected as a possible inhibitor of the Ebola virus infection. The results of an additional docking analysis as well as experimental results showing measurable anti-Ebola effect of ibuprofen in cell culture suggest ibuprofen as a promising molecular template for the development of drugs for treatment of the infection by Ebola virus.
T2  - Frontiers in Bioscience - Landmark
T1  - Ibuprofen as a template molecule for drug design against Ebola virus
VL  - 23
IS  - 5
SP  - 947
EP  - 953
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7695
ER  - 

@article{
author = "Paessler, Slobodan and Huang, Cheng and Senćanski, Milan V. and Veljković, Nevena V. and Perović, Vladimir R. and Glišić, Sanja and Veljković, Veljko",
year = "2018",
abstract = "The Ebola virus outbreak in West Africa 2015 and Congo 2017, point out an urgent need for development of drugs against this important pathogen. Previously, by repurposing virtual screening of 6438 drugs from DrugBank, ibuprofen was selected as a possible inhibitor of the Ebola virus infection. The results of an additional docking analysis as well as experimental results showing measurable anti-Ebola effect of ibuprofen in cell culture suggest ibuprofen as a promising molecular template for the development of drugs for treatment of the infection by Ebola virus.",
journal = "Frontiers in Bioscience - Landmark",
title = "Ibuprofen as a template molecule for drug design against Ebola virus",
volume = "23",
number = "5",
pages = "947-953",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7695"
}

Paessler, S., Huang, C., Senćanski, M. V., Veljković, N. V., Perović, V. R., Glišić, S.,& Veljković, V.. (2018). Ibuprofen as a template molecule for drug design against Ebola virus. in Frontiers in Bioscience - Landmark, 23(5), 947-953.
https://hdl.handle.net/21.15107/rcub_vinar_7695

Paessler S, Huang C, Senćanski MV, Veljković NV, Perović VR, Glišić S, Veljković V. Ibuprofen as a template molecule for drug design against Ebola virus. in Frontiers in Bioscience - Landmark. 2018;23(5):947-953.
https://hdl.handle.net/21.15107/rcub_vinar_7695 .

Paessler, Slobodan, Huang, Cheng, Senćanski, Milan V., Veljković, Nevena V., Perović, Vladimir R., Glišić, Sanja, Veljković, Veljko, "Ibuprofen as a template molecule for drug design against Ebola virus" in Frontiers in Bioscience - Landmark, 23, no. 5 (2018):947-953,
https://hdl.handle.net/21.15107/rcub_vinar_7695 .

TY  - CONF
AU  - Perović, Vladimir R.
AU  - Gemović, Branislava S.
AU  - Veljković, Veljko
AU  - Glišić, Sanja
AU  - Veljković, Nevena V.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10770
AB  - Summary. Coding genetic variants can have profound effects on protein function. Computational tools for the prediction of these effects are used to complement and guide experimental biological studies. Phylogenetic analyses that determine the evolutionary relationship among related sequences are commonly used to distinguish between functionally significant and insignificant gene variations. Here, we have reviewed applications of the non-alignment sequence analyses method for phylogenetic analyses, ISTREE. Furthermore, we assessed how an unsupervised ISTREE-d3 method based on the universal d3 measure responds to this task compared to supervised and semi-supervised ISTREE methods that were previously used in two studies. The findings presented here suggest that ISTREE-d3 can efficiently substitute for the corresponding supervised models, given that it is more suitable for automatic applications. In conclusion, the ISTREE-d3 method has a broad biological relevance and represents a promising approach in functional assessment of coding gene variations.
C3  - Biologica Serbica
T1  - Annotation Of The Functional Impact Of Coding Genetic Variants
VL  - 39
IS  - 1
SP  - 74
EP  - 82
DO  - 10.5281/zenodo.826908
ER  - 

@conference{
author = "Perović, Vladimir R. and Gemović, Branislava S. and Veljković, Veljko and Glišić, Sanja and Veljković, Nevena V.",
year = "2017",
abstract = "Summary. Coding genetic variants can have profound effects on protein function. Computational tools for the prediction of these effects are used to complement and guide experimental biological studies. Phylogenetic analyses that determine the evolutionary relationship among related sequences are commonly used to distinguish between functionally significant and insignificant gene variations. Here, we have reviewed applications of the non-alignment sequence analyses method for phylogenetic analyses, ISTREE. Furthermore, we assessed how an unsupervised ISTREE-d3 method based on the universal d3 measure responds to this task compared to supervised and semi-supervised ISTREE methods that were previously used in two studies. The findings presented here suggest that ISTREE-d3 can efficiently substitute for the corresponding supervised models, given that it is more suitable for automatic applications. In conclusion, the ISTREE-d3 method has a broad biological relevance and represents a promising approach in functional assessment of coding gene variations.",
journal = "Biologica Serbica",
title = "Annotation Of The Functional Impact Of Coding Genetic Variants",
volume = "39",
number = "1",
pages = "74-82",
doi = "10.5281/zenodo.826908"
}

Perović, V. R., Gemović, B. S., Veljković, V., Glišić, S.,& Veljković, N. V.. (2017). Annotation Of The Functional Impact Of Coding Genetic Variants. in Biologica Serbica, 39(1), 74-82.
https://doi.org/10.5281/zenodo.826908

Perović VR, Gemović BS, Veljković V, Glišić S, Veljković NV. Annotation Of The Functional Impact Of Coding Genetic Variants. in Biologica Serbica. 2017;39(1):74-82.
doi:10.5281/zenodo.826908 .

Perović, Vladimir R., Gemović, Branislava S., Veljković, Veljko, Glišić, Sanja, Veljković, Nevena V., "Annotation Of The Functional Impact Of Coding Genetic Variants" in Biologica Serbica, 39, no. 1 (2017):74-82,
https://doi.org/10.5281/zenodo.826908 . .

TY  - JOUR
AU  - Veljković, Veljko
AU  - Glišić, Sanja
AU  - Perović, Vladimir R.
AU  - Veljković, Nevena V.
AU  - Nicolson, Garth L.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1372
AB  - Background: Numerous in vitro and in vivo studies, in addition to clinical data, demonstrate that pomegranate juice can prevent or slow-down the progression of some types of cancers. Despite the well-documented effect of pomegranate ingredients on neoplastic changes, the molecular mechanism(s) underlying this phenomenon remains elusive. Methods: For the study of pomegranate ingredients the electron-ion interaction potential (EIIP) and the average quasi valence number (AQVN) were used. These molecular descriptors can be used to describe the long-range intermolecular interactions in biological systems and can identify substances with strong electron-acceptor properties. In this study, candidate human proteins interacting with pomegranate flavonoids have been analyzed by the informational spectrum method (ISM). This represents a virtual spectroscopy method for studying protein molecular interactions. Results: Our analysis indicates that the anti-cancer properties of pomegranate juice can be ascribed to the strong electron-acceptor properties of its chemical ingredients. This analysis also suggests that pomegranate flavonoids inhibit the NF-kappaB (NF-kappa B) pathway, which plays a critical role in the pathogenesis of cancer. Conclusion: The results offer a possible explanation for an important molecular mechanism underlying the anticancer activity of pomegranate ingredients, which could also serve as a basis for the development of new therapeutic compositions of food supplements with pomegranate-like anticancer properties.
T2  - Functional Foods in Health and Disease
T1  - Pomegranate (Punica granatum): a natural source for the development of therapeutic compositions of food supplements with anticancer activities based on electron acceptor molecular characteristics
VL  - 6
IS  - 12
SP  - 769
EP  - 787
DO  - 10.31989/ffhd.v6i12.289
ER  - 

@article{
author = "Veljković, Veljko and Glišić, Sanja and Perović, Vladimir R. and Veljković, Nevena V. and Nicolson, Garth L.",
year = "2016",
abstract = "Background: Numerous in vitro and in vivo studies, in addition to clinical data, demonstrate that pomegranate juice can prevent or slow-down the progression of some types of cancers. Despite the well-documented effect of pomegranate ingredients on neoplastic changes, the molecular mechanism(s) underlying this phenomenon remains elusive. Methods: For the study of pomegranate ingredients the electron-ion interaction potential (EIIP) and the average quasi valence number (AQVN) were used. These molecular descriptors can be used to describe the long-range intermolecular interactions in biological systems and can identify substances with strong electron-acceptor properties. In this study, candidate human proteins interacting with pomegranate flavonoids have been analyzed by the informational spectrum method (ISM). This represents a virtual spectroscopy method for studying protein molecular interactions. Results: Our analysis indicates that the anti-cancer properties of pomegranate juice can be ascribed to the strong electron-acceptor properties of its chemical ingredients. This analysis also suggests that pomegranate flavonoids inhibit the NF-kappaB (NF-kappa B) pathway, which plays a critical role in the pathogenesis of cancer. Conclusion: The results offer a possible explanation for an important molecular mechanism underlying the anticancer activity of pomegranate ingredients, which could also serve as a basis for the development of new therapeutic compositions of food supplements with pomegranate-like anticancer properties.",
journal = "Functional Foods in Health and Disease",
title = "Pomegranate (Punica granatum): a natural source for the development of therapeutic compositions of food supplements with anticancer activities based on electron acceptor molecular characteristics",
volume = "6",
number = "12",
pages = "769-787",
doi = "10.31989/ffhd.v6i12.289"
}

Veljković, V., Glišić, S., Perović, V. R., Veljković, N. V.,& Nicolson, G. L.. (2016). Pomegranate (Punica granatum): a natural source for the development of therapeutic compositions of food supplements with anticancer activities based on electron acceptor molecular characteristics. in Functional Foods in Health and Disease, 6(12), 769-787.
https://doi.org/10.31989/ffhd.v6i12.289

Veljković V, Glišić S, Perović VR, Veljković NV, Nicolson GL. Pomegranate (Punica granatum): a natural source for the development of therapeutic compositions of food supplements with anticancer activities based on electron acceptor molecular characteristics. in Functional Foods in Health and Disease. 2016;6(12):769-787.
doi:10.31989/ffhd.v6i12.289 .

Veljković, Veljko, Glišić, Sanja, Perović, Vladimir R., Veljković, Nevena V., Nicolson, Garth L., "Pomegranate (Punica granatum): a natural source for the development of therapeutic compositions of food supplements with anticancer activities based on electron acceptor molecular characteristics" in Functional Foods in Health and Disease, 6, no. 12 (2016):769-787,
https://doi.org/10.31989/ffhd.v6i12.289 . .

TY  - JOUR
AU  - Jiang, Yuxiang
AU  - Gemović, Branislava S.
AU  - Glišić, Sanja
AU  - Perović, Vladimir R.
AU  - Veljković, Veljko
AU  - Veljković, Nevena V.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1244
AB  - Background: A major bottleneck in our understanding of the molecular underpinnings of life is the assignment of function to proteins. While molecular experiments provide the most reliable annotation of proteins, their relatively low throughput and restricted purview have led to an increasing role for computational function prediction. However, assessing methods for protein function prediction and tracking progress in the field remain challenging. Results: We conducted the second critical assessment of functional annotation (CAFA), a timed challenge to assess computational methods that automatically assign protein function. We evaluated 126 methods from 56 research groups for their ability to predict biological functions using Gene Ontology and gene-disease associations using Human Phenotype Ontology on a set of 3681 proteins from 18 species. CAFA2 featured expanded analysis compared with CAFA1, with regards to data set size, variety, and assessment metrics. To review progress in the field, the analysis compared the best methods from CAFA1 to those of CAFA2. Conclusions: The top-performing methods in CAFA2 outperformed those from CAFA1. This increased accuracy can be attributed to a combination of the growing number of experimental annotations and improved methods for function prediction. The assessment also revealed that the definition of top-performing algorithms is ontology specific, that different performance metrics can be used to probe the nature of accurate predictions, and the relative diversity of predictions in the biological process and human phenotype ontologies. While there was methodological improvement between CAFA1 and CAFA2, the interpretation of results and usefulness of individual methods remain context-dependent.
T2  - Genome Biology
T1  - An expanded evaluation of protein function prediction methods shows an improvement in accuracy
VL  - 17
DO  - 10.1186/s13059-016-1037-6
ER  - 

@article{
author = "Jiang, Yuxiang and Gemović, Branislava S. and Glišić, Sanja and Perović, Vladimir R. and Veljković, Veljko and Veljković, Nevena V.",
year = "2016",
abstract = "Background: A major bottleneck in our understanding of the molecular underpinnings of life is the assignment of function to proteins. While molecular experiments provide the most reliable annotation of proteins, their relatively low throughput and restricted purview have led to an increasing role for computational function prediction. However, assessing methods for protein function prediction and tracking progress in the field remain challenging. Results: We conducted the second critical assessment of functional annotation (CAFA), a timed challenge to assess computational methods that automatically assign protein function. We evaluated 126 methods from 56 research groups for their ability to predict biological functions using Gene Ontology and gene-disease associations using Human Phenotype Ontology on a set of 3681 proteins from 18 species. CAFA2 featured expanded analysis compared with CAFA1, with regards to data set size, variety, and assessment metrics. To review progress in the field, the analysis compared the best methods from CAFA1 to those of CAFA2. Conclusions: The top-performing methods in CAFA2 outperformed those from CAFA1. This increased accuracy can be attributed to a combination of the growing number of experimental annotations and improved methods for function prediction. The assessment also revealed that the definition of top-performing algorithms is ontology specific, that different performance metrics can be used to probe the nature of accurate predictions, and the relative diversity of predictions in the biological process and human phenotype ontologies. While there was methodological improvement between CAFA1 and CAFA2, the interpretation of results and usefulness of individual methods remain context-dependent.",
journal = "Genome Biology",
title = "An expanded evaluation of protein function prediction methods shows an improvement in accuracy",
volume = "17",
doi = "10.1186/s13059-016-1037-6"
}

Jiang, Y., Gemović, B. S., Glišić, S., Perović, V. R., Veljković, V.,& Veljković, N. V.. (2016). An expanded evaluation of protein function prediction methods shows an improvement in accuracy. in Genome Biology, 17.
https://doi.org/10.1186/s13059-016-1037-6

Jiang Y, Gemović BS, Glišić S, Perović VR, Veljković V, Veljković NV. An expanded evaluation of protein function prediction methods shows an improvement in accuracy. in Genome Biology. 2016;17.
doi:10.1186/s13059-016-1037-6 .

Jiang, Yuxiang, Gemović, Branislava S., Glišić, Sanja, Perović, Vladimir R., Veljković, Veljko, Veljković, Nevena V., "An expanded evaluation of protein function prediction methods shows an improvement in accuracy" in Genome Biology, 17 (2016),
https://doi.org/10.1186/s13059-016-1037-6 . .

TY  - JOUR
AU  - Veljković, Veljko
AU  - Veljković, Nevena V.
AU  - Paessler, Slobodan
AU  - Goeijenbier, Marco
AU  - Perović, Vladimir R.
AU  - Glišić, Sanja
AU  - Muller, Claude P.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1300
AB  - Influenza A virus (IAV) subtypes against which little or no pre-existing immunity exists in humans represent a serious threat to global public health. Monitoring of IAV in animal hosts is essential for early and rapid detection of potential pandemic IAV strains to prevent their spread. Recently, the increased pandemic potential of the avian-like swine H1N1 IAV A/swine/Guangdong/104/2013 has been suggested. The virus is infectious in humans and the general population seems to lack neutralizing antibodies against this virus. Here we present an in silico analysis that shows a strong human propensity of this swine virus further confirming its pandemic potential. We suggest mutations which would further enhance its human propensity. We also propose conserved antigenic determinants which could serve as a component of a prepandemic vaccine. The bioinformatics tool, which can be used to further monitor the evolution of swine influenza viruses towards a pandemic virus, are described here and are made publically available (http://www.vin.bg.ac.rs/180/tools/iav_ mon.php;http://www.biomedprotection.com/iav_mon.php).
T2  - PLOS One
T1  - Predicted Enhanced Human Propensity of Current Avian-Like H1N1 Swine Influenza Virus from China
VL  - 11
IS  - 11
DO  - 10.1371/journal.pone.0165451
ER  - 

@article{
author = "Veljković, Veljko and Veljković, Nevena V. and Paessler, Slobodan and Goeijenbier, Marco and Perović, Vladimir R. and Glišić, Sanja and Muller, Claude P.",
year = "2016",
abstract = "Influenza A virus (IAV) subtypes against which little or no pre-existing immunity exists in humans represent a serious threat to global public health. Monitoring of IAV in animal hosts is essential for early and rapid detection of potential pandemic IAV strains to prevent their spread. Recently, the increased pandemic potential of the avian-like swine H1N1 IAV A/swine/Guangdong/104/2013 has been suggested. The virus is infectious in humans and the general population seems to lack neutralizing antibodies against this virus. Here we present an in silico analysis that shows a strong human propensity of this swine virus further confirming its pandemic potential. We suggest mutations which would further enhance its human propensity. We also propose conserved antigenic determinants which could serve as a component of a prepandemic vaccine. The bioinformatics tool, which can be used to further monitor the evolution of swine influenza viruses towards a pandemic virus, are described here and are made publically available (http://www.vin.bg.ac.rs/180/tools/iav_ mon.php;http://www.biomedprotection.com/iav_mon.php).",
journal = "PLOS One",
title = "Predicted Enhanced Human Propensity of Current Avian-Like H1N1 Swine Influenza Virus from China",
volume = "11",
number = "11",
doi = "10.1371/journal.pone.0165451"
}

Veljković, V., Veljković, N. V., Paessler, S., Goeijenbier, M., Perović, V. R., Glišić, S.,& Muller, C. P.. (2016). Predicted Enhanced Human Propensity of Current Avian-Like H1N1 Swine Influenza Virus from China. in PLOS One, 11(11).
https://doi.org/10.1371/journal.pone.0165451

Veljković V, Veljković NV, Paessler S, Goeijenbier M, Perović VR, Glišić S, Muller CP. Predicted Enhanced Human Propensity of Current Avian-Like H1N1 Swine Influenza Virus from China. in PLOS One. 2016;11(11).
doi:10.1371/journal.pone.0165451 .

Veljković, Veljko, Veljković, Nevena V., Paessler, Slobodan, Goeijenbier, Marco, Perović, Vladimir R., Glišić, Sanja, Muller, Claude P., "Predicted Enhanced Human Propensity of Current Avian-Like H1N1 Swine Influenza Virus from China" in PLOS One, 11, no. 11 (2016),
https://doi.org/10.1371/journal.pone.0165451 . .

TY  - JOUR
AU  - Glišić, Sanja
AU  - Paessler, Slobodan
AU  - Veljković, Nevena V.
AU  - Perović, Vladimir R.
AU  - Prljić, Jelena
AU  - Veljković, Veljko
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/709
AB  - Introduction: The Ebola 2014/2015 outbreak has had devastating effects on the people living in West Africa. The spread of the disease in endemic countries and the potential introduction of sporadic cases in other continents points out the global health threat of Ebola virus disease (EVD). Despite the urgent need for treating EVD, there are no approved treatments. Given the lack of treatments available, alternative therapeutic strategies have had to be used. Areas covered: This article summarizes the unregistered therapeutics that were used to treat patients during the Ebola 2014/2015 outbreak, in addition to approaches used for the selection of candidate drugs. The article also proposes potential theoretical criterion for use in virtual screening of molecular libraries for candidate Ebola drugs. Expert opinion: In the absence of approved therapeutics for EVD, experimental drugs have had to be used. The repurposing of approved drugs for the treatment of EVD, as an alternative therapeutic strategy, has also been suggested. Screening in vitro- and in sllico-approved drugs revealed several promising candidates but further testing is required to test their efficacy. All these therapeutic approaches are, however, only short-term solutions and there is still an urgent need for the development of specific drugs for the current and future outbreaks.
T2  - Expert Opinion on Drug Discovery
T1  - Improving attrition rates in Ebola virus drug discovery
VL  - 10
IS  - 9
SP  - 1025
EP  - 1032
DO  - 10.1517/17460441.2015.1062872
ER  - 

@article{
author = "Glišić, Sanja and Paessler, Slobodan and Veljković, Nevena V. and Perović, Vladimir R. and Prljić, Jelena and Veljković, Veljko",
year = "2015",
abstract = "Introduction: The Ebola 2014/2015 outbreak has had devastating effects on the people living in West Africa. The spread of the disease in endemic countries and the potential introduction of sporadic cases in other continents points out the global health threat of Ebola virus disease (EVD). Despite the urgent need for treating EVD, there are no approved treatments. Given the lack of treatments available, alternative therapeutic strategies have had to be used. Areas covered: This article summarizes the unregistered therapeutics that were used to treat patients during the Ebola 2014/2015 outbreak, in addition to approaches used for the selection of candidate drugs. The article also proposes potential theoretical criterion for use in virtual screening of molecular libraries for candidate Ebola drugs. Expert opinion: In the absence of approved therapeutics for EVD, experimental drugs have had to be used. The repurposing of approved drugs for the treatment of EVD, as an alternative therapeutic strategy, has also been suggested. Screening in vitro- and in sllico-approved drugs revealed several promising candidates but further testing is required to test their efficacy. All these therapeutic approaches are, however, only short-term solutions and there is still an urgent need for the development of specific drugs for the current and future outbreaks.",
journal = "Expert Opinion on Drug Discovery",
title = "Improving attrition rates in Ebola virus drug discovery",
volume = "10",
number = "9",
pages = "1025-1032",
doi = "10.1517/17460441.2015.1062872"
}

Glišić, S., Paessler, S., Veljković, N. V., Perović, V. R., Prljić, J.,& Veljković, V.. (2015). Improving attrition rates in Ebola virus drug discovery. in Expert Opinion on Drug Discovery, 10(9), 1025-1032.
https://doi.org/10.1517/17460441.2015.1062872

Glišić S, Paessler S, Veljković NV, Perović VR, Prljić J, Veljković V. Improving attrition rates in Ebola virus drug discovery. in Expert Opinion on Drug Discovery. 2015;10(9):1025-1032.
doi:10.1517/17460441.2015.1062872 .

Glišić, Sanja, Paessler, Slobodan, Veljković, Nevena V., Perović, Vladimir R., Prljić, Jelena, Veljković, Veljko, "Improving attrition rates in Ebola virus drug discovery" in Expert Opinion on Drug Discovery, 10, no. 9 (2015):1025-1032,
https://doi.org/10.1517/17460441.2015.1062872 . .

TY  - JOUR
AU  - Veljković, Nevena V.
AU  - Vučićević, Jelica
AU  - Tassini, Sabrina
AU  - Glišić, Sanja
AU  - Veljković, Veljko
AU  - Radi, Marco
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/558
AB  - Introduction: Maraviroc is a first-in-class antiretroviral (ARV) drug acting on a host cell target (CCR5), which blocks the entry of the HIV virus into the cell. Maraviroc is currently indicated for combination ARV treatment in adults infected only with CCR5-tropic HIV-1. Areas covered: This drug discovery case history focuses on the key studies that led to the discovery and approval of maraviroc, as well as on post-launch clinical reports. The article is based on the data reported in published preclinical and clinical studies, conference posters and on drug package data. Expert opinion: The profound understanding of HIVs entry mechanisms has provided a strong biological rationale for targeting the chemokine receptor CCR5. The CCR5-antagonist mariviroc, with its unique mode of action and excellent safety profile, is an important therapeutic option for HIV patients. In general, the authors believe that targeting host factors is a useful approach for combating new and re-emerging transmissible diseases, as well as pathogens that easily become resistant to common antiviral drugs. Maraviroc, offering a potent and safe cellular receptor-mediated pharmacological response to HIV, has paved the way for the development of a new generation of host-targeting antivirals.
T2  - Expert Opinion on Drug Discovery
T1  - Preclinical discovery and development of maraviroc for the treatment of HIV
VL  - 10
IS  - 6
SP  - 671
EP  - 684
DO  - 10.1517/17460441.2015.1041497
ER  - 

@article{
author = "Veljković, Nevena V. and Vučićević, Jelica and Tassini, Sabrina and Glišić, Sanja and Veljković, Veljko and Radi, Marco",
year = "2015",
abstract = "Introduction: Maraviroc is a first-in-class antiretroviral (ARV) drug acting on a host cell target (CCR5), which blocks the entry of the HIV virus into the cell. Maraviroc is currently indicated for combination ARV treatment in adults infected only with CCR5-tropic HIV-1. Areas covered: This drug discovery case history focuses on the key studies that led to the discovery and approval of maraviroc, as well as on post-launch clinical reports. The article is based on the data reported in published preclinical and clinical studies, conference posters and on drug package data. Expert opinion: The profound understanding of HIVs entry mechanisms has provided a strong biological rationale for targeting the chemokine receptor CCR5. The CCR5-antagonist mariviroc, with its unique mode of action and excellent safety profile, is an important therapeutic option for HIV patients. In general, the authors believe that targeting host factors is a useful approach for combating new and re-emerging transmissible diseases, as well as pathogens that easily become resistant to common antiviral drugs. Maraviroc, offering a potent and safe cellular receptor-mediated pharmacological response to HIV, has paved the way for the development of a new generation of host-targeting antivirals.",
journal = "Expert Opinion on Drug Discovery",
title = "Preclinical discovery and development of maraviroc for the treatment of HIV",
volume = "10",
number = "6",
pages = "671-684",
doi = "10.1517/17460441.2015.1041497"
}

Veljković, N. V., Vučićević, J., Tassini, S., Glišić, S., Veljković, V.,& Radi, M.. (2015). Preclinical discovery and development of maraviroc for the treatment of HIV. in Expert Opinion on Drug Discovery, 10(6), 671-684.
https://doi.org/10.1517/17460441.2015.1041497

Veljković NV, Vučićević J, Tassini S, Glišić S, Veljković V, Radi M. Preclinical discovery and development of maraviroc for the treatment of HIV. in Expert Opinion on Drug Discovery. 2015;10(6):671-684.
doi:10.1517/17460441.2015.1041497 .

Veljković, Nevena V., Vučićević, Jelica, Tassini, Sabrina, Glišić, Sanja, Veljković, Veljko, Radi, Marco, "Preclinical discovery and development of maraviroc for the treatment of HIV" in Expert Opinion on Drug Discovery, 10, no. 6 (2015):671-684,
https://doi.org/10.1517/17460441.2015.1041497 . .

TY  - JOUR
AU  - Veljković, Veljko
AU  - Glišić, Sanja
AU  - Muller, Claude P.
AU  - Scotch, Matthew
AU  - Branch, Donald R.
AU  - Perović, Vladimir R.
AU  - Senćanski, Milan V.
AU  - Veljković, Nevena V.
AU  - Colombatti, Alfonso
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/423
AB  - The worst Ebola virus (EV) outbreak in history has hit Liberia, Sierra Leone and Guinea hardest and the trend lines in this crisis are grave, and now represents a global public health threat concern. Limited therapeutic and/or prophylactic options are available for people suffering from Ebola virus disease (EVD) and further complicate the situation. Previous studies suggested that the EV glycoprotein (GP) is the main determinant causing structural damage of endothelial cells that triggers the hemorrhagic diathesis, but molecular mechanisms underlying this phenomenon remains elusive. Using the informational spectrum method (ISM), a virtual spectroscopy method for analysis of the protein-protein interactions, the interaction of GP with endothelial extracellular matrix (ECM) was investigated. Presented results of this in silico study suggest that Elastin Microfibril Interface Located Proteins (EMILINs) are involved in interaction between GP and ECM. This finding could contribute to a better understanding of EV/endothelium interaction and its role in pathogenesis, prevention and therapy of EVD.
T2  - Frontiers in Microbiology
T1  - In silico analysis suggests interaction between Ebola virus and the extracellular matrix
VL  - 6
DO  - 10.3389/fmicb.2015.00135
ER  - 

@article{
author = "Veljković, Veljko and Glišić, Sanja and Muller, Claude P. and Scotch, Matthew and Branch, Donald R. and Perović, Vladimir R. and Senćanski, Milan V. and Veljković, Nevena V. and Colombatti, Alfonso",
year = "2015",
abstract = "The worst Ebola virus (EV) outbreak in history has hit Liberia, Sierra Leone and Guinea hardest and the trend lines in this crisis are grave, and now represents a global public health threat concern. Limited therapeutic and/or prophylactic options are available for people suffering from Ebola virus disease (EVD) and further complicate the situation. Previous studies suggested that the EV glycoprotein (GP) is the main determinant causing structural damage of endothelial cells that triggers the hemorrhagic diathesis, but molecular mechanisms underlying this phenomenon remains elusive. Using the informational spectrum method (ISM), a virtual spectroscopy method for analysis of the protein-protein interactions, the interaction of GP with endothelial extracellular matrix (ECM) was investigated. Presented results of this in silico study suggest that Elastin Microfibril Interface Located Proteins (EMILINs) are involved in interaction between GP and ECM. This finding could contribute to a better understanding of EV/endothelium interaction and its role in pathogenesis, prevention and therapy of EVD.",
journal = "Frontiers in Microbiology",
title = "In silico analysis suggests interaction between Ebola virus and the extracellular matrix",
volume = "6",
doi = "10.3389/fmicb.2015.00135"
}

Veljković, V., Glišić, S., Muller, C. P., Scotch, M., Branch, D. R., Perović, V. R., Senćanski, M. V., Veljković, N. V.,& Colombatti, A.. (2015). In silico analysis suggests interaction between Ebola virus and the extracellular matrix. in Frontiers in Microbiology, 6.
https://doi.org/10.3389/fmicb.2015.00135

Veljković V, Glišić S, Muller CP, Scotch M, Branch DR, Perović VR, Senćanski MV, Veljković NV, Colombatti A. In silico analysis suggests interaction between Ebola virus and the extracellular matrix. in Frontiers in Microbiology. 2015;6.
doi:10.3389/fmicb.2015.00135 .

Veljković, Veljko, Glišić, Sanja, Muller, Claude P., Scotch, Matthew, Branch, Donald R., Perović, Vladimir R., Senćanski, Milan V., Veljković, Nevena V., Colombatti, Alfonso, "In silico analysis suggests interaction between Ebola virus and the extracellular matrix" in Frontiers in Microbiology, 6 (2015),
https://doi.org/10.3389/fmicb.2015.00135 . .

TY  - JOUR
AU  - Veljković, Veljko
AU  - Paessler, Slobodan
AU  - Glišić, Sanja
AU  - Prljić, Jelena
AU  - Perović, Vladimir R.
AU  - Veljković, Nevena V.
AU  - Scotch, Matthew
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/863
AB  - A key factor in the effectiveness of the seasonal influenza vaccine is its immunological compatibility with the circulating viruses during the season. Here we propose a new bioinformatics approach for analysis of influenza viruses which could be used as an efficient tool for selection of vaccine viruses, assessment of the effectiveness of seasonal influenza vaccines, and prediction of the epidemic/pandemic potential of novel influenza viruses.
T2  - Frontiers in Microbiology
T1  - Evolution of 2014/15 H3N2 Influenza Viruses Circulating in US: Consequences for Vaccine Effectiveness and Possible New Pandemic
VL  - 6
DO  - 10.3389/fmicb.2015.01456
ER  - 

@article{
author = "Veljković, Veljko and Paessler, Slobodan and Glišić, Sanja and Prljić, Jelena and Perović, Vladimir R. and Veljković, Nevena V. and Scotch, Matthew",
year = "2015",
abstract = "A key factor in the effectiveness of the seasonal influenza vaccine is its immunological compatibility with the circulating viruses during the season. Here we propose a new bioinformatics approach for analysis of influenza viruses which could be used as an efficient tool for selection of vaccine viruses, assessment of the effectiveness of seasonal influenza vaccines, and prediction of the epidemic/pandemic potential of novel influenza viruses.",
journal = "Frontiers in Microbiology",
title = "Evolution of 2014/15 H3N2 Influenza Viruses Circulating in US: Consequences for Vaccine Effectiveness and Possible New Pandemic",
volume = "6",
doi = "10.3389/fmicb.2015.01456"
}

Veljković, V., Paessler, S., Glišić, S., Prljić, J., Perović, V. R., Veljković, N. V.,& Scotch, M.. (2015). Evolution of 2014/15 H3N2 Influenza Viruses Circulating in US: Consequences for Vaccine Effectiveness and Possible New Pandemic. in Frontiers in Microbiology, 6.
https://doi.org/10.3389/fmicb.2015.01456

Veljković V, Paessler S, Glišić S, Prljić J, Perović VR, Veljković NV, Scotch M. Evolution of 2014/15 H3N2 Influenza Viruses Circulating in US: Consequences for Vaccine Effectiveness and Possible New Pandemic. in Frontiers in Microbiology. 2015;6.
doi:10.3389/fmicb.2015.01456 .

Veljković, Veljko, Paessler, Slobodan, Glišić, Sanja, Prljić, Jelena, Perović, Vladimir R., Veljković, Nevena V., Scotch, Matthew, "Evolution of 2014/15 H3N2 Influenza Viruses Circulating in US: Consequences for Vaccine Effectiveness and Possible New Pandemic" in Frontiers in Microbiology, 6 (2015),
https://doi.org/10.3389/fmicb.2015.01456 . .

TY  - JOUR
AU  - Schmier, Sonja
AU  - Mostafa, Ahmed
AU  - Haarmann, Thomas
AU  - Bannert, Norbert
AU  - Ziebuhr, John
AU  - Veljković, Veljko
AU  - Dietrich, Ursula
AU  - Pleschka, Stephan
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/616
AB  - Newly emerging influenza A viruses (IAV) pose a major threat to human health by causing seasonal epidemics and/or pandemics, the latter often facilitated by the lack of pre-existing immunity in the general population. Early recognition of candidate pandemic influenza viruses (CPIV) is of crucial importance for restricting virus transmission and developing appropriate therapeutic and prophylactic strategies including effective vaccines. Often, the pandemic potential of newly emerging IAV is only fully recognized once the virus starts to spread efficiently causing serious disease in humans. Here, we used a novel phylogenetic algorithm based on the informational spectrum method (ISM) to identify potential CPIV by predicting mutations in the viral hemagglutinin (HA) gene that are likely to (differentially) affect critical interactions between the HA protein and target cells from bird and human origin, respectively. Predictions were subsequently validated by generating pseudotyped retrovirus particles and genetically engineered IAV containing these mutations and characterizing potential effects on virus entry and replication in cells expressing human and avian IAV receptors, respectively. Our data suggest that the ISM-based algorithm is suitable to identify CPIV among IAV strains that are circulating in animal hosts and thus may be a new tool for assessing pandemic risks associated with specific strains.
T2  - Scientific Reports
T1  - In Silico Prediction and Experimental Confirmation of HA Residues Conferring Enhanced Human Receptor Specificity of H5N1 Influenza A Viruses
VL  - 5
DO  - 10.1038/srep11434
ER  - 

@article{
author = "Schmier, Sonja and Mostafa, Ahmed and Haarmann, Thomas and Bannert, Norbert and Ziebuhr, John and Veljković, Veljko and Dietrich, Ursula and Pleschka, Stephan",
year = "2015",
abstract = "Newly emerging influenza A viruses (IAV) pose a major threat to human health by causing seasonal epidemics and/or pandemics, the latter often facilitated by the lack of pre-existing immunity in the general population. Early recognition of candidate pandemic influenza viruses (CPIV) is of crucial importance for restricting virus transmission and developing appropriate therapeutic and prophylactic strategies including effective vaccines. Often, the pandemic potential of newly emerging IAV is only fully recognized once the virus starts to spread efficiently causing serious disease in humans. Here, we used a novel phylogenetic algorithm based on the informational spectrum method (ISM) to identify potential CPIV by predicting mutations in the viral hemagglutinin (HA) gene that are likely to (differentially) affect critical interactions between the HA protein and target cells from bird and human origin, respectively. Predictions were subsequently validated by generating pseudotyped retrovirus particles and genetically engineered IAV containing these mutations and characterizing potential effects on virus entry and replication in cells expressing human and avian IAV receptors, respectively. Our data suggest that the ISM-based algorithm is suitable to identify CPIV among IAV strains that are circulating in animal hosts and thus may be a new tool for assessing pandemic risks associated with specific strains.",
journal = "Scientific Reports",
title = "In Silico Prediction and Experimental Confirmation of HA Residues Conferring Enhanced Human Receptor Specificity of H5N1 Influenza A Viruses",
volume = "5",
doi = "10.1038/srep11434"
}

Schmier, S., Mostafa, A., Haarmann, T., Bannert, N., Ziebuhr, J., Veljković, V., Dietrich, U.,& Pleschka, S.. (2015). In Silico Prediction and Experimental Confirmation of HA Residues Conferring Enhanced Human Receptor Specificity of H5N1 Influenza A Viruses. in Scientific Reports, 5.
https://doi.org/10.1038/srep11434

Schmier S, Mostafa A, Haarmann T, Bannert N, Ziebuhr J, Veljković V, Dietrich U, Pleschka S. In Silico Prediction and Experimental Confirmation of HA Residues Conferring Enhanced Human Receptor Specificity of H5N1 Influenza A Viruses. in Scientific Reports. 2015;5.
doi:10.1038/srep11434 .

Schmier, Sonja, Mostafa, Ahmed, Haarmann, Thomas, Bannert, Norbert, Ziebuhr, John, Veljković, Veljko, Dietrich, Ursula, Pleschka, Stephan, "In Silico Prediction and Experimental Confirmation of HA Residues Conferring Enhanced Human Receptor Specificity of H5N1 Influenza A Viruses" in Scientific Reports, 5 (2015),
https://doi.org/10.1038/srep11434 . .

TY  - JOUR
AU  - Vujicic, Ana Djordjevic
AU  - Gemović, Branislava S.
AU  - Veljković, Veljko
AU  - Glišić, Sanja
AU  - Veljković, Nevena V.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5960
AB  - Background/Aim. High sera reactivity with a peptide derived from human immunodeficiency virus HIV-I envelope protein gp120, NMI., correlate with non-progressive HIV-1 infection and also may have protective role in breast and prostate cancer. We also detected a low NTM1 reactive antibodies titer in healthy HIV negative sera and showed that antibody levels can be significantly increased with vigorous physical activity. However, the immune system seems to be unresponsive or tolerant to this peptide, implicating that the NTM1 sequence encompasses or overlaps a certain innate immune epitope. The aim of this study was to present evidences that NTM1 binding antibodies are components of innate immune humoral response, by confirming their presence in sera of newborn babies. For this purpose we collected a set of 225 innate antigen sequences reported in the literature and screened it for candidate antigens with the highest sequence and spectral similarity to NTM1 derived from HIV-1 gp120. Methods. Sera from 18 newborns were tested using ELISA, with peptide NTM1. Sequences from innate antigen database were aligned by an EMBOSS Water bioinformatics tool. Results. We identified NTM1 reactive antibodies in sera of HIV negative newborn babies. Further, in order to identify which of already known innate antigens are the most similar to NTM1 peptide we screened innate immune antigen sequence database collected from the literature. This screening revealed that the most similar sequence are ribonucleoproteins RO60, in addition to previously identified N-terminus of vasoactive intestinal peptide. Conclusion. The results of this study confirm the hypothesis that NTM1 recognizing antibodies are a part of humoral innate immune response. Further, computational similarity screening revealed a vasoactive intestinal peptide and RO60 as the most similar sequences and the strongest candidate antigens. In the light of the presented results, it is appealing that testing blood reactivity at birth, with specific innate antigens, particularly a vasoactive intestinal peptide, can reveal the potential to develop- or boost protective immune response in breast and prostate cancer and HIV infection later in life.
T2  - Vojnosanitetski pregled
T1  - Natural autoantibodies in healthy neonatals recognizing a peptide derived from the second conserved region of HIV-1 gp120
VL  - 71
IS  - 4
SP  - 352
EP  - 361
DO  - 10.2298/VSP1404352D
ER  - 

@article{
author = "Vujicic, Ana Djordjevic and Gemović, Branislava S. and Veljković, Veljko and Glišić, Sanja and Veljković, Nevena V.",
year = "2014",
abstract = "Background/Aim. High sera reactivity with a peptide derived from human immunodeficiency virus HIV-I envelope protein gp120, NMI., correlate with non-progressive HIV-1 infection and also may have protective role in breast and prostate cancer. We also detected a low NTM1 reactive antibodies titer in healthy HIV negative sera and showed that antibody levels can be significantly increased with vigorous physical activity. However, the immune system seems to be unresponsive or tolerant to this peptide, implicating that the NTM1 sequence encompasses or overlaps a certain innate immune epitope. The aim of this study was to present evidences that NTM1 binding antibodies are components of innate immune humoral response, by confirming their presence in sera of newborn babies. For this purpose we collected a set of 225 innate antigen sequences reported in the literature and screened it for candidate antigens with the highest sequence and spectral similarity to NTM1 derived from HIV-1 gp120. Methods. Sera from 18 newborns were tested using ELISA, with peptide NTM1. Sequences from innate antigen database were aligned by an EMBOSS Water bioinformatics tool. Results. We identified NTM1 reactive antibodies in sera of HIV negative newborn babies. Further, in order to identify which of already known innate antigens are the most similar to NTM1 peptide we screened innate immune antigen sequence database collected from the literature. This screening revealed that the most similar sequence are ribonucleoproteins RO60, in addition to previously identified N-terminus of vasoactive intestinal peptide. Conclusion. The results of this study confirm the hypothesis that NTM1 recognizing antibodies are a part of humoral innate immune response. Further, computational similarity screening revealed a vasoactive intestinal peptide and RO60 as the most similar sequences and the strongest candidate antigens. In the light of the presented results, it is appealing that testing blood reactivity at birth, with specific innate antigens, particularly a vasoactive intestinal peptide, can reveal the potential to develop- or boost protective immune response in breast and prostate cancer and HIV infection later in life.",
journal = "Vojnosanitetski pregled",
title = "Natural autoantibodies in healthy neonatals recognizing a peptide derived from the second conserved region of HIV-1 gp120",
volume = "71",
number = "4",
pages = "352-361",
doi = "10.2298/VSP1404352D"
}

Vujicic, A. D., Gemović, B. S., Veljković, V., Glišić, S.,& Veljković, N. V.. (2014). Natural autoantibodies in healthy neonatals recognizing a peptide derived from the second conserved region of HIV-1 gp120. in Vojnosanitetski pregled, 71(4), 352-361.
https://doi.org/10.2298/VSP1404352D

Vujicic AD, Gemović BS, Veljković V, Glišić S, Veljković NV. Natural autoantibodies in healthy neonatals recognizing a peptide derived from the second conserved region of HIV-1 gp120. in Vojnosanitetski pregled. 2014;71(4):352-361.
doi:10.2298/VSP1404352D .

Vujicic, Ana Djordjevic, Gemović, Branislava S., Veljković, Veljko, Glišić, Sanja, Veljković, Nevena V., "Natural autoantibodies in healthy neonatals recognizing a peptide derived from the second conserved region of HIV-1 gp120" in Vojnosanitetski pregled, 71, no. 4 (2014):352-361,
https://doi.org/10.2298/VSP1404352D . .

TY  - JOUR
AU  - Veljković, Veljko
AU  - Glišić, Sanja
AU  - Veljković, Nevena V.
AU  - Bojić, Tijana
AU  - Dietrich, Ursula
AU  - Perović, Vladimir R.
AU  - Colombatti, Alfonso
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/229
AB  - Despite plausible evidence for beneficial effects of the vaccination against influenza in cardiovascular diseases (CVD) very limited studies have been carried out to explain the molecular mechanism of this phenomenon. Using the informational spectrum method (ISM), a virtual spectroscopy method for analysis of protein-protein interactions, the bradykinin 2 receptor (BKB2R) was identified as a principal host protein which could mediate molecular processes underlying the cardioprotective effect of influenza vaccines. Based on this finding we suggest that some antibodies elicited by influenza vaccines act as agonists, which activate a BKB2R-associated signaling pathway contributing to the protection against CVD. The ISM analysis of 14 influenza viruses, which were used as components of seasonal vaccines, revealed four vaccine viruses A/Beijing/262/95(H1N1), A/NewCaledonia/20/1999(H1N1), A/Christchurch/28/2003(H3N2) and A/Perth/16/2009(H3N2), which could be suited best for further studies on the cardioprotective effect of influenza vaccines. (C) 2014 Elsevier Ltd. All rights reserved.
T2  - Vaccine
T1  - Influenza vaccine as prevention for cardiovascular diseases: Possible molecular mechanism
VL  - 32
IS  - 48
SP  - 6569
EP  - 6575
DO  - 10.1016/j.vaccine.2014.07.007
ER  - 

@article{
author = "Veljković, Veljko and Glišić, Sanja and Veljković, Nevena V. and Bojić, Tijana and Dietrich, Ursula and Perović, Vladimir R. and Colombatti, Alfonso",
year = "2014",
abstract = "Despite plausible evidence for beneficial effects of the vaccination against influenza in cardiovascular diseases (CVD) very limited studies have been carried out to explain the molecular mechanism of this phenomenon. Using the informational spectrum method (ISM), a virtual spectroscopy method for analysis of protein-protein interactions, the bradykinin 2 receptor (BKB2R) was identified as a principal host protein which could mediate molecular processes underlying the cardioprotective effect of influenza vaccines. Based on this finding we suggest that some antibodies elicited by influenza vaccines act as agonists, which activate a BKB2R-associated signaling pathway contributing to the protection against CVD. The ISM analysis of 14 influenza viruses, which were used as components of seasonal vaccines, revealed four vaccine viruses A/Beijing/262/95(H1N1), A/NewCaledonia/20/1999(H1N1), A/Christchurch/28/2003(H3N2) and A/Perth/16/2009(H3N2), which could be suited best for further studies on the cardioprotective effect of influenza vaccines. (C) 2014 Elsevier Ltd. All rights reserved.",
journal = "Vaccine",
title = "Influenza vaccine as prevention for cardiovascular diseases: Possible molecular mechanism",
volume = "32",
number = "48",
pages = "6569-6575",
doi = "10.1016/j.vaccine.2014.07.007"
}

Veljković, V., Glišić, S., Veljković, N. V., Bojić, T., Dietrich, U., Perović, V. R.,& Colombatti, A.. (2014). Influenza vaccine as prevention for cardiovascular diseases: Possible molecular mechanism. in Vaccine, 32(48), 6569-6575.
https://doi.org/10.1016/j.vaccine.2014.07.007

Veljković V, Glišić S, Veljković NV, Bojić T, Dietrich U, Perović VR, Colombatti A. Influenza vaccine as prevention for cardiovascular diseases: Possible molecular mechanism. in Vaccine. 2014;32(48):6569-6575.
doi:10.1016/j.vaccine.2014.07.007 .

Veljković, Veljko, Glišić, Sanja, Veljković, Nevena V., Bojić, Tijana, Dietrich, Ursula, Perović, Vladimir R., Colombatti, Alfonso, "Influenza vaccine as prevention for cardiovascular diseases: Possible molecular mechanism" in Vaccine, 32, no. 48 (2014):6569-6575,
https://doi.org/10.1016/j.vaccine.2014.07.007 . .

TY  - JOUR
AU  - Maga, Giovanni
AU  - Veljković, Nevena V.
AU  - Crespan, Emmanuele
AU  - Spadari, Silvio
AU  - Prljić, Jelena
AU  - Perović, Vladimir R.
AU  - Glišić, Sanja
AU  - Veljković, Veljko
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5227
AB  - Introduction: Recently, the new concept of the long-range intermolecular interactions in biological systems has been proposed. Combined use of molecular modeling techniques and the screening techniques based on the long-range interaction concept could significantly improve and accelerate discovery of new HIV drugs. However, any hit identified in silico needs to be characterized with respect to its biological target by enzymatic studies. Combined use of the in silico screening and the enzymatic studies allows an efficient selection of new anti-HIV drugs. Areas covered: The focus of this article is on the in silico screening of molecular libraries for candidate new HIV drugs, which is based on the molecular descriptors determining the long-range interaction between the drugs and their therapeutic target. This article also reviews the techniques for enzyme kinetic studies which are required for optimization of in silico selected candidate anti-HIV drugs. Expert opinion: The novel approach of combining in silico screening techniques with enzymatic studies enables the accurate measurement of the quantitative descriptors of ligand-enzyme interactions. This novel method is a powerful tool for new anti-HIV drug discovery which can also reduce the drug development costs.
T2  - Expert Opinion on Drug Discovery
T1  - New in silico and conventional in vitro approaches to advance HIV drug discovery and design
VL  - 8
IS  - 1
SP  - 83
EP  - 92
DO  - 10.1517/17460441.2013.741118
ER  - 

@article{
author = "Maga, Giovanni and Veljković, Nevena V. and Crespan, Emmanuele and Spadari, Silvio and Prljić, Jelena and Perović, Vladimir R. and Glišić, Sanja and Veljković, Veljko",
year = "2013",
abstract = "Introduction: Recently, the new concept of the long-range intermolecular interactions in biological systems has been proposed. Combined use of molecular modeling techniques and the screening techniques based on the long-range interaction concept could significantly improve and accelerate discovery of new HIV drugs. However, any hit identified in silico needs to be characterized with respect to its biological target by enzymatic studies. Combined use of the in silico screening and the enzymatic studies allows an efficient selection of new anti-HIV drugs. Areas covered: The focus of this article is on the in silico screening of molecular libraries for candidate new HIV drugs, which is based on the molecular descriptors determining the long-range interaction between the drugs and their therapeutic target. This article also reviews the techniques for enzyme kinetic studies which are required for optimization of in silico selected candidate anti-HIV drugs. Expert opinion: The novel approach of combining in silico screening techniques with enzymatic studies enables the accurate measurement of the quantitative descriptors of ligand-enzyme interactions. This novel method is a powerful tool for new anti-HIV drug discovery which can also reduce the drug development costs.",
journal = "Expert Opinion on Drug Discovery",
title = "New in silico and conventional in vitro approaches to advance HIV drug discovery and design",
volume = "8",
number = "1",
pages = "83-92",
doi = "10.1517/17460441.2013.741118"
}

Maga, G., Veljković, N. V., Crespan, E., Spadari, S., Prljić, J., Perović, V. R., Glišić, S.,& Veljković, V.. (2013). New in silico and conventional in vitro approaches to advance HIV drug discovery and design. in Expert Opinion on Drug Discovery, 8(1), 83-92.
https://doi.org/10.1517/17460441.2013.741118

Maga G, Veljković NV, Crespan E, Spadari S, Prljić J, Perović VR, Glišić S, Veljković V. New in silico and conventional in vitro approaches to advance HIV drug discovery and design. in Expert Opinion on Drug Discovery. 2013;8(1):83-92.
doi:10.1517/17460441.2013.741118 .

Maga, Giovanni, Veljković, Nevena V., Crespan, Emmanuele, Spadari, Silvio, Prljić, Jelena, Perović, Vladimir R., Glišić, Sanja, Veljković, Veljko, "New in silico and conventional in vitro approaches to advance HIV drug discovery and design" in Expert Opinion on Drug Discovery, 8, no. 1 (2013):83-92,
https://doi.org/10.1517/17460441.2013.741118 . .

TY  - JOUR
AU  - Perović, Vladimir R.
AU  - Muller, Claude P.
AU  - Niman, Henry L.
AU  - Veljković, Nevena V.
AU  - Dietrich, Ursula
AU  - Tosic, Dusan D.
AU  - Glišić, Sanja
AU  - Veljković, Veljko
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5491
AB  - Years of endemic infections with highly pathogenic avian influenza (HPAI) A subtype H5N1 virus in poultry and high numbers of infections in humans provide ample opportunity in Egypt for H5N1-HPAIV to develop pandemic potential. In an effort to better understand the viral determinants that facilitate human infections of the Egyptian H5N1-HPAIVvirus, we developed a new phylogenetic algorithm based on a new distance measure derived from the informational spectrum method (ISM). This new approach, which describes functional aspects of the evolution of the hemagglutinin subunit 1 (HA1), revealed a growing group G2 of H5N1-HPAIV in Egypt after 2009 that acquired new informational spectrum (IS) properties suggestive of an increased human tropism and pandemic potential. While in 2006 all viruses in Egypt belonged to the G1 group, by 2011 these viruses were virtually replaced by G2 viruses. All of the G2 viruses displayed four characteristic mutations (D43N, S120(D,N), (S,L)129 Delta and I151T), three of which were previously reported to increase binding to the human receptor. Already in 2006-2008 G2 viruses were significantly (p LT 0.02) more often found in humans than expected from their overall prevalence and this further increased in 2009-2011 (p LT 0.007). Our approach also identified viruses that acquired additional mutations that we predict to further enhance their human tropism. The extensive evolution of Egyptian H5N1-HPAIV towards a preferential human tropism underlines an urgent need to closely monitor these viruses with respect to molecular determinants of virulence.
T2  - PLOS One
T1  - Novel Phylogenetic Algorithm to Monitor Human Tropism in Egyptian H5N1-HPAIV Reveals Evolution toward Efficient Human-to-Human Transmission
VL  - 8
IS  - 4
DO  - 10.1371/journal.pone.0061572
ER  - 

@article{
author = "Perović, Vladimir R. and Muller, Claude P. and Niman, Henry L. and Veljković, Nevena V. and Dietrich, Ursula and Tosic, Dusan D. and Glišić, Sanja and Veljković, Veljko",
year = "2013",
abstract = "Years of endemic infections with highly pathogenic avian influenza (HPAI) A subtype H5N1 virus in poultry and high numbers of infections in humans provide ample opportunity in Egypt for H5N1-HPAIV to develop pandemic potential. In an effort to better understand the viral determinants that facilitate human infections of the Egyptian H5N1-HPAIVvirus, we developed a new phylogenetic algorithm based on a new distance measure derived from the informational spectrum method (ISM). This new approach, which describes functional aspects of the evolution of the hemagglutinin subunit 1 (HA1), revealed a growing group G2 of H5N1-HPAIV in Egypt after 2009 that acquired new informational spectrum (IS) properties suggestive of an increased human tropism and pandemic potential. While in 2006 all viruses in Egypt belonged to the G1 group, by 2011 these viruses were virtually replaced by G2 viruses. All of the G2 viruses displayed four characteristic mutations (D43N, S120(D,N), (S,L)129 Delta and I151T), three of which were previously reported to increase binding to the human receptor. Already in 2006-2008 G2 viruses were significantly (p LT 0.02) more often found in humans than expected from their overall prevalence and this further increased in 2009-2011 (p LT 0.007). Our approach also identified viruses that acquired additional mutations that we predict to further enhance their human tropism. The extensive evolution of Egyptian H5N1-HPAIV towards a preferential human tropism underlines an urgent need to closely monitor these viruses with respect to molecular determinants of virulence.",
journal = "PLOS One",
title = "Novel Phylogenetic Algorithm to Monitor Human Tropism in Egyptian H5N1-HPAIV Reveals Evolution toward Efficient Human-to-Human Transmission",
volume = "8",
number = "4",
doi = "10.1371/journal.pone.0061572"
}

Perović, V. R., Muller, C. P., Niman, H. L., Veljković, N. V., Dietrich, U., Tosic, D. D., Glišić, S.,& Veljković, V.. (2013). Novel Phylogenetic Algorithm to Monitor Human Tropism in Egyptian H5N1-HPAIV Reveals Evolution toward Efficient Human-to-Human Transmission. in PLOS One, 8(4).
https://doi.org/10.1371/journal.pone.0061572

Perović VR, Muller CP, Niman HL, Veljković NV, Dietrich U, Tosic DD, Glišić S, Veljković V. Novel Phylogenetic Algorithm to Monitor Human Tropism in Egyptian H5N1-HPAIV Reveals Evolution toward Efficient Human-to-Human Transmission. in PLOS One. 2013;8(4).
doi:10.1371/journal.pone.0061572 .

Perović, Vladimir R., Muller, Claude P., Niman, Henry L., Veljković, Nevena V., Dietrich, Ursula, Tosic, Dusan D., Glišić, Sanja, Veljković, Veljko, "Novel Phylogenetic Algorithm to Monitor Human Tropism in Egyptian H5N1-HPAIV Reveals Evolution toward Efficient Human-to-Human Transmission" in PLOS One, 8, no. 4 (2013),
https://doi.org/10.1371/journal.pone.0061572 . .

TY  - JOUR
AU  - Veljković, Nevena V.
AU  - Glišić, Sanja
AU  - Prljić, Jelena
AU  - Perović, Vladimir R.
AU  - Veljković, Veljko
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5758
AB  - Highly active antiretroviral therapy (HAART) dramatically has changed the course of HIV infection. Currently, this therapy involves the use of agents from at least two distinct classes of antivirals: a protease inhibitor in combination with two nucleoside/nucleotide reverse transcriptase inhibitors (N(t)RTIs), or a non-nucleoside reverse transcriptase inhibitor (NNRTI) in combination with NRTIs. Recently, the third family of antivirals started to be used clinically, with the advent of enfuvirtide, the first fusion inhibitor. This broad spectrum of anti-HIV agents recently was extended with compounds inhibiting HIV integrase and vital entry. But these advances did not come without a cost: there were the short-and long-term drug toxicities, emergence of drug resistance, and persistence of viral reservoirs. For these reasons, there is a pressing need for novel anti-HIV drugs, particularly those that have a novel action mechanism, as these might be less likely to show cross-resistance with current therapies. The field of bioinformatics has become a major part of the drug discovery pipeline playing a key role in improvement and acceleration of the time and money consuming process of the drug development. Here we review the application of the EIIP/AQVN (Electron-Ion Interaction Potential, EIIP; Average Quasi Valence Number, AQVN) bioinformatics concept in the development of new anti-HIV drugs and propose a simple theoretical criterion for a virtual screening of molecular libraries for promising lead anti-HIV compounds and refinement of selected lead compounds in order to increase their biological activity.
T2  - Current Pharmaceutical Biotechnology
T1  - Simple and General Criterion for in silico Screening of Candidate HIV Drugs
VL  - 14
IS  - 5
SP  - 561
EP  - 569
DO  - 10.2174/138920101405131111105301
ER  - 

@article{
author = "Veljković, Nevena V. and Glišić, Sanja and Prljić, Jelena and Perović, Vladimir R. and Veljković, Veljko",
year = "2013",
abstract = "Highly active antiretroviral therapy (HAART) dramatically has changed the course of HIV infection. Currently, this therapy involves the use of agents from at least two distinct classes of antivirals: a protease inhibitor in combination with two nucleoside/nucleotide reverse transcriptase inhibitors (N(t)RTIs), or a non-nucleoside reverse transcriptase inhibitor (NNRTI) in combination with NRTIs. Recently, the third family of antivirals started to be used clinically, with the advent of enfuvirtide, the first fusion inhibitor. This broad spectrum of anti-HIV agents recently was extended with compounds inhibiting HIV integrase and vital entry. But these advances did not come without a cost: there were the short-and long-term drug toxicities, emergence of drug resistance, and persistence of viral reservoirs. For these reasons, there is a pressing need for novel anti-HIV drugs, particularly those that have a novel action mechanism, as these might be less likely to show cross-resistance with current therapies. The field of bioinformatics has become a major part of the drug discovery pipeline playing a key role in improvement and acceleration of the time and money consuming process of the drug development. Here we review the application of the EIIP/AQVN (Electron-Ion Interaction Potential, EIIP; Average Quasi Valence Number, AQVN) bioinformatics concept in the development of new anti-HIV drugs and propose a simple theoretical criterion for a virtual screening of molecular libraries for promising lead anti-HIV compounds and refinement of selected lead compounds in order to increase their biological activity.",
journal = "Current Pharmaceutical Biotechnology",
title = "Simple and General Criterion for in silico Screening of Candidate HIV Drugs",
volume = "14",
number = "5",
pages = "561-569",
doi = "10.2174/138920101405131111105301"
}

Veljković, N. V., Glišić, S., Prljić, J., Perović, V. R.,& Veljković, V.. (2013). Simple and General Criterion for in silico Screening of Candidate HIV Drugs. in Current Pharmaceutical Biotechnology, 14(5), 561-569.
https://doi.org/10.2174/138920101405131111105301

Veljković NV, Glišić S, Prljić J, Perović VR, Veljković V. Simple and General Criterion for in silico Screening of Candidate HIV Drugs. in Current Pharmaceutical Biotechnology. 2013;14(5):561-569.
doi:10.2174/138920101405131111105301 .

Veljković, Nevena V., Glišić, Sanja, Prljić, Jelena, Perović, Vladimir R., Veljković, Veljko, "Simple and General Criterion for in silico Screening of Candidate HIV Drugs" in Current Pharmaceutical Biotechnology, 14, no. 5 (2013):561-569,
https://doi.org/10.2174/138920101405131111105301 . .

TY  - JOUR
AU  - Glišić, Sanja
AU  - Veljković, Nevena V.
AU  - Jovanović-Ćupić, Snežana P.
AU  - Vasiljevic, Nada
AU  - Prljić, Jelena
AU  - Gemović, Branislava S.
AU  - Perović, Vladimir R.
AU  - Veljković, Veljko
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4755
AB  - Hepatitis C virus (HCV) infection is a major and rising global health problem, affecting about 170 million people worldwide. The current standard of care treatment with interferon alpha and ribavirin in patients with the genotype 1 infection, the most frequent genotype in the USA and Western Europe, leads to a successful outcome in only about 50% of individuals. Accurate prediction of hepatitis C treatment response is of great benefit to patients and clinicians. The informational spectrum method, a virtual spectroscopy method for structure/function analysis of nucleotide and protein sequences, is applied here for the identification of the conserved information of the HCV proteins that correlate with the combination therapy outcome. Among the HCV proteins that we have analyzed the informational property of the p7 of HCV genotype 1b was best related to the therapy outcome. On the basis of these results, a simple bioinformatics criterion that could be useful in assessment of the response of HCV-infected patients to the combination therapy has been proposed.
T2  - Protein Journal
T1  - Assessment of Hepatitis C Virus Protein Sequences with Regard to Interferon/Ribavirin Combination Therapy Response in Patients with HCV Genotype 1b
VL  - 31
IS  - 2
SP  - 129
EP  - 136
DO  - 10.1007/s10930-011-9381-6
ER  - 

@article{
author = "Glišić, Sanja and Veljković, Nevena V. and Jovanović-Ćupić, Snežana P. and Vasiljevic, Nada and Prljić, Jelena and Gemović, Branislava S. and Perović, Vladimir R. and Veljković, Veljko",
year = "2012",
abstract = "Hepatitis C virus (HCV) infection is a major and rising global health problem, affecting about 170 million people worldwide. The current standard of care treatment with interferon alpha and ribavirin in patients with the genotype 1 infection, the most frequent genotype in the USA and Western Europe, leads to a successful outcome in only about 50% of individuals. Accurate prediction of hepatitis C treatment response is of great benefit to patients and clinicians. The informational spectrum method, a virtual spectroscopy method for structure/function analysis of nucleotide and protein sequences, is applied here for the identification of the conserved information of the HCV proteins that correlate with the combination therapy outcome. Among the HCV proteins that we have analyzed the informational property of the p7 of HCV genotype 1b was best related to the therapy outcome. On the basis of these results, a simple bioinformatics criterion that could be useful in assessment of the response of HCV-infected patients to the combination therapy has been proposed.",
journal = "Protein Journal",
title = "Assessment of Hepatitis C Virus Protein Sequences with Regard to Interferon/Ribavirin Combination Therapy Response in Patients with HCV Genotype 1b",
volume = "31",
number = "2",
pages = "129-136",
doi = "10.1007/s10930-011-9381-6"
}

Glišić, S., Veljković, N. V., Jovanović-Ćupić, S. P., Vasiljevic, N., Prljić, J., Gemović, B. S., Perović, V. R.,& Veljković, V.. (2012). Assessment of Hepatitis C Virus Protein Sequences with Regard to Interferon/Ribavirin Combination Therapy Response in Patients with HCV Genotype 1b. in Protein Journal, 31(2), 129-136.
https://doi.org/10.1007/s10930-011-9381-6

Glišić S, Veljković NV, Jovanović-Ćupić SP, Vasiljevic N, Prljić J, Gemović BS, Perović VR, Veljković V. Assessment of Hepatitis C Virus Protein Sequences with Regard to Interferon/Ribavirin Combination Therapy Response in Patients with HCV Genotype 1b. in Protein Journal. 2012;31(2):129-136.
doi:10.1007/s10930-011-9381-6 .

Glišić, Sanja, Veljković, Nevena V., Jovanović-Ćupić, Snežana P., Vasiljevic, Nada, Prljić, Jelena, Gemović, Branislava S., Perović, Vladimir R., Veljković, Veljko, "Assessment of Hepatitis C Virus Protein Sequences with Regard to Interferon/Ribavirin Combination Therapy Response in Patients with HCV Genotype 1b" in Protein Journal, 31, no. 2 (2012):129-136,
https://doi.org/10.1007/s10930-011-9381-6 . .

TY  - JOUR
AU  - Vergara-Alert, Julia
AU  - Argilaguet, Jordi M.
AU  - Busquets, Nuria
AU  - Ballester, Maria
AU  - Martin-Valls, Gerard E.
AU  - Rivas, Raquel
AU  - Lopez-Soria, Sergio
AU  - Solanes, David
AU  - Majo, Natalia
AU  - Segales, Joaquim
AU  - Veljković, Veljko
AU  - Rodriguez, Fernando
AU  - Darji, Ayub
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4956
AB  - Outbreaks involving either H5N1 or H1N1 influenza viruses (IV) have recently become an increasing threat to cause potential pandemics. Pigs have an important role in this aspect. As reflected in the 2009 human H1N1 pandemia, they may act as a vehicle for mixing and generating new assortments of viruses potentially pathogenic to animals and humans. Lack of universal vaccines against the highly variable influenza virus forces scientists to continuously design vaccines a la carte, which is an expensive and risky practice overall when dealing with virulent strains. Therefore, we focused our efforts on developing a broadly protective influenza vaccine based on the Informational Spectrum Method (ISM). This theoretical prediction allows the selection of highly conserved peptide sequences from within the hemagglutinin subunit 1 protein (HA1) from either H5 or H1 viruses which are located in the flanking region of the HA binding site and with the potential to elicit broader immune responses than conventional vaccines. Confirming the theoretical predictions, immunization of conventional farm pigs with the synthetic peptides induced humoral responses in every single pig. The fact that the induced antibodies were able to recognize in vitro heterologous influenza viruses such as the pandemic H1N1 virus (pH1N1), two swine influenza field isolates (SwH1N1 and SwH3N2) and a H5N1 highly pathogenic avian virus, confirm the broad recognition of the antibodies induced. Unexpectedly, all pigs also showed T-cell responses that not only recognized the specific peptides, but also the pH1N1 virus. Finally, a partial effect on the kinetics of virus clearance was observed after the intranasal infection with the pH1N1 virus, setting forth the groundwork for the design of peptide-based vaccines against influenza viruses. Further insights into the understanding of the mechanisms involved in the protection afforded will be necessary to optimize future vaccine formulations.
T2  - PLOS One
T1  - Conserved Synthetic Peptides from the Hemagglutinin of Influenza Viruses Induce Broad Humoral and T-Cell Responses in a Pig Model
VL  - 7
IS  - 7
DO  - 10.1371/journal.pone.0040524
ER  - 

@article{
author = "Vergara-Alert, Julia and Argilaguet, Jordi M. and Busquets, Nuria and Ballester, Maria and Martin-Valls, Gerard E. and Rivas, Raquel and Lopez-Soria, Sergio and Solanes, David and Majo, Natalia and Segales, Joaquim and Veljković, Veljko and Rodriguez, Fernando and Darji, Ayub",
year = "2012",
abstract = "Outbreaks involving either H5N1 or H1N1 influenza viruses (IV) have recently become an increasing threat to cause potential pandemics. Pigs have an important role in this aspect. As reflected in the 2009 human H1N1 pandemia, they may act as a vehicle for mixing and generating new assortments of viruses potentially pathogenic to animals and humans. Lack of universal vaccines against the highly variable influenza virus forces scientists to continuously design vaccines a la carte, which is an expensive and risky practice overall when dealing with virulent strains. Therefore, we focused our efforts on developing a broadly protective influenza vaccine based on the Informational Spectrum Method (ISM). This theoretical prediction allows the selection of highly conserved peptide sequences from within the hemagglutinin subunit 1 protein (HA1) from either H5 or H1 viruses which are located in the flanking region of the HA binding site and with the potential to elicit broader immune responses than conventional vaccines. Confirming the theoretical predictions, immunization of conventional farm pigs with the synthetic peptides induced humoral responses in every single pig. The fact that the induced antibodies were able to recognize in vitro heterologous influenza viruses such as the pandemic H1N1 virus (pH1N1), two swine influenza field isolates (SwH1N1 and SwH3N2) and a H5N1 highly pathogenic avian virus, confirm the broad recognition of the antibodies induced. Unexpectedly, all pigs also showed T-cell responses that not only recognized the specific peptides, but also the pH1N1 virus. Finally, a partial effect on the kinetics of virus clearance was observed after the intranasal infection with the pH1N1 virus, setting forth the groundwork for the design of peptide-based vaccines against influenza viruses. Further insights into the understanding of the mechanisms involved in the protection afforded will be necessary to optimize future vaccine formulations.",
journal = "PLOS One",
title = "Conserved Synthetic Peptides from the Hemagglutinin of Influenza Viruses Induce Broad Humoral and T-Cell Responses in a Pig Model",
volume = "7",
number = "7",
doi = "10.1371/journal.pone.0040524"
}

Vergara-Alert, J., Argilaguet, J. M., Busquets, N., Ballester, M., Martin-Valls, G. E., Rivas, R., Lopez-Soria, S., Solanes, D., Majo, N., Segales, J., Veljković, V., Rodriguez, F.,& Darji, A.. (2012). Conserved Synthetic Peptides from the Hemagglutinin of Influenza Viruses Induce Broad Humoral and T-Cell Responses in a Pig Model. in PLOS One, 7(7).
https://doi.org/10.1371/journal.pone.0040524

Vergara-Alert J, Argilaguet JM, Busquets N, Ballester M, Martin-Valls GE, Rivas R, Lopez-Soria S, Solanes D, Majo N, Segales J, Veljković V, Rodriguez F, Darji A. Conserved Synthetic Peptides from the Hemagglutinin of Influenza Viruses Induce Broad Humoral and T-Cell Responses in a Pig Model. in PLOS One. 2012;7(7).
doi:10.1371/journal.pone.0040524 .

Vergara-Alert, Julia, Argilaguet, Jordi M., Busquets, Nuria, Ballester, Maria, Martin-Valls, Gerard E., Rivas, Raquel, Lopez-Soria, Sergio, Solanes, David, Majo, Natalia, Segales, Joaquim, Veljković, Veljko, Rodriguez, Fernando, Darji, Ayub, "Conserved Synthetic Peptides from the Hemagglutinin of Influenza Viruses Induce Broad Humoral and T-Cell Responses in a Pig Model" in PLOS One, 7, no. 7 (2012),
https://doi.org/10.1371/journal.pone.0040524 . .

TY  - JOUR
AU  - Veljković, Milena
AU  - Dopsaj, Violeta
AU  - Dopsaj, Milivoj
AU  - Branch, Donald R.
AU  - Veljković, Nevena V.
AU  - Sakarellos-Daitsiotis, Maria M.
AU  - Veljković, Veljko
AU  - Glišić, Sanja
AU  - Colombatti, Alfonso
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4613
AB  - Background: There is convincing evidence from numerous clinical and epidemiological studies that physical activity can reduce the risk for breast and prostate cancer. The biological mechanisms underlying this phenomenon remain elusive. Herein we suggest a role for naturally produced antibodies reactive with the vasoactive intestinal peptide (VIP) in the suppression of breast and prostate cancer, which we believe could offer a possible molecular mechanism underlying control of these cancers by physical exercise. Methodology and Results: We found that sera from individuals having breast and prostate cancers have decreased titers of VIP natural antibodies as demonstrated by a lower reactivity against peptide NTM1, having similar informational and structural properties as VIP. In contrast, sera collected from elite athletes, exhibited titers of natural NTM1-reactive antibodies that are significantly increased, suggesting that physical activity boosts production of these antibodies. Significance: Presented results suggest that physical exercise stimulates production of natural anti-VIP antibodies and likely results in suppression of VIP. This, in turn, may play a protective role against breast and prostate cancers. Physical exercise should be further investigated as a potential tool in the treatment of these diseases.
T2  - PLOS One
T1  - Physical Activity and Natural Anti-VIP Antibodies: Potential Role in Breast and Prostate Cancer Therapy
VL  - 6
IS  - 11
DO  - 10.1371/journal.pone.0028304
ER  - 

@article{
author = "Veljković, Milena and Dopsaj, Violeta and Dopsaj, Milivoj and Branch, Donald R. and Veljković, Nevena V. and Sakarellos-Daitsiotis, Maria M. and Veljković, Veljko and Glišić, Sanja and Colombatti, Alfonso",
year = "2011",
abstract = "Background: There is convincing evidence from numerous clinical and epidemiological studies that physical activity can reduce the risk for breast and prostate cancer. The biological mechanisms underlying this phenomenon remain elusive. Herein we suggest a role for naturally produced antibodies reactive with the vasoactive intestinal peptide (VIP) in the suppression of breast and prostate cancer, which we believe could offer a possible molecular mechanism underlying control of these cancers by physical exercise. Methodology and Results: We found that sera from individuals having breast and prostate cancers have decreased titers of VIP natural antibodies as demonstrated by a lower reactivity against peptide NTM1, having similar informational and structural properties as VIP. In contrast, sera collected from elite athletes, exhibited titers of natural NTM1-reactive antibodies that are significantly increased, suggesting that physical activity boosts production of these antibodies. Significance: Presented results suggest that physical exercise stimulates production of natural anti-VIP antibodies and likely results in suppression of VIP. This, in turn, may play a protective role against breast and prostate cancers. Physical exercise should be further investigated as a potential tool in the treatment of these diseases.",
journal = "PLOS One",
title = "Physical Activity and Natural Anti-VIP Antibodies: Potential Role in Breast and Prostate Cancer Therapy",
volume = "6",
number = "11",
doi = "10.1371/journal.pone.0028304"
}

Veljković, M., Dopsaj, V., Dopsaj, M., Branch, D. R., Veljković, N. V., Sakarellos-Daitsiotis, M. M., Veljković, V., Glišić, S.,& Colombatti, A.. (2011). Physical Activity and Natural Anti-VIP Antibodies: Potential Role in Breast and Prostate Cancer Therapy. in PLOS One, 6(11).
https://doi.org/10.1371/journal.pone.0028304

Veljković M, Dopsaj V, Dopsaj M, Branch DR, Veljković NV, Sakarellos-Daitsiotis MM, Veljković V, Glišić S, Colombatti A. Physical Activity and Natural Anti-VIP Antibodies: Potential Role in Breast and Prostate Cancer Therapy. in PLOS One. 2011;6(11).
doi:10.1371/journal.pone.0028304 .

Veljković, Milena, Dopsaj, Violeta, Dopsaj, Milivoj, Branch, Donald R., Veljković, Nevena V., Sakarellos-Daitsiotis, Maria M., Veljković, Veljko, Glišić, Sanja, Colombatti, Alfonso, "Physical Activity and Natural Anti-VIP Antibodies: Potential Role in Breast and Prostate Cancer Therapy" in PLOS One, 6, no. 11 (2011),
https://doi.org/10.1371/journal.pone.0028304 . .

TY  - JOUR
AU  - Veljković, Nevena V.
AU  - Glišić, Sanja
AU  - Perović, Vladimir R.
AU  - Veljković, Veljko
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4595
AB  - Introduction: Long-range intermolecular interactions (interactions at distances between 100 and 1000 A) play an important role in the interaction between drugs and therapeutic targets, and design techniques based on this concept could significantly improve and accelerate new drug discovery. Understanding these long-range intermolecular interactions will also help further our understanding of the molecular mechanisms and the underlying basic biological processes. Areas covered: This article looks at the physical bases of long-range intermolecular interactions in biological systems with a brief review of the literature data to support this concept. The article also gives some examples of techniques used in drug discovery that were based on the long-range intermolecular interaction concept. Expert opinion: The electron-ion interaction potential (EIIP) and average quasivalence number (AQVN) concepts shed new light on the role of long-range intermolecular interactions in biological systems. Further research of physicochemical mechanisms underlying long-range interactions between biological molecules is necessary for a better understanding of the basic biological processes. The addition of the computer-aided design techniques based on the EIIP/AQVN concept to the research and development will lead not only to a significant reduction in cost but also to an acceleration in the development of new drugs.
T2  - Expert Opinion on Drug Discovery
T1  - The role of long-range intermolecular interactions in discovery of new drugs
VL  - 6
IS  - 12
SP  - 1263
EP  - 1270
DO  - 10.1517/17460441.2012.638280
ER  - 

@article{
author = "Veljković, Nevena V. and Glišić, Sanja and Perović, Vladimir R. and Veljković, Veljko",
year = "2011",
abstract = "Introduction: Long-range intermolecular interactions (interactions at distances between 100 and 1000 A) play an important role in the interaction between drugs and therapeutic targets, and design techniques based on this concept could significantly improve and accelerate new drug discovery. Understanding these long-range intermolecular interactions will also help further our understanding of the molecular mechanisms and the underlying basic biological processes. Areas covered: This article looks at the physical bases of long-range intermolecular interactions in biological systems with a brief review of the literature data to support this concept. The article also gives some examples of techniques used in drug discovery that were based on the long-range intermolecular interaction concept. Expert opinion: The electron-ion interaction potential (EIIP) and average quasivalence number (AQVN) concepts shed new light on the role of long-range intermolecular interactions in biological systems. Further research of physicochemical mechanisms underlying long-range interactions between biological molecules is necessary for a better understanding of the basic biological processes. The addition of the computer-aided design techniques based on the EIIP/AQVN concept to the research and development will lead not only to a significant reduction in cost but also to an acceleration in the development of new drugs.",
journal = "Expert Opinion on Drug Discovery",
title = "The role of long-range intermolecular interactions in discovery of new drugs",
volume = "6",
number = "12",
pages = "1263-1270",
doi = "10.1517/17460441.2012.638280"
}

Veljković, N. V., Glišić, S., Perović, V. R.,& Veljković, V.. (2011). The role of long-range intermolecular interactions in discovery of new drugs. in Expert Opinion on Drug Discovery, 6(12), 1263-1270.
https://doi.org/10.1517/17460441.2012.638280

Veljković NV, Glišić S, Perović VR, Veljković V. The role of long-range intermolecular interactions in discovery of new drugs. in Expert Opinion on Drug Discovery. 2011;6(12):1263-1270.
doi:10.1517/17460441.2012.638280 .

Veljković, Nevena V., Glišić, Sanja, Perović, Vladimir R., Veljković, Veljko, "The role of long-range intermolecular interactions in discovery of new drugs" in Expert Opinion on Drug Discovery, 6, no. 12 (2011):1263-1270,
https://doi.org/10.1517/17460441.2012.638280 . .

TY  - JOUR
AU  - Veljković, Milena
AU  - Branch, Donald R.
AU  - Dopsaj, Violeta
AU  - Veljković, Veljko
AU  - Veljković, Nevena V.
AU  - Glišić, Sanja
AU  - Colombatti, Alfonso
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4495
AB  - Objectives: The incidence of non-AIDS-defining cancer is remarkably higher in HIV-infected than in the general population. In contrast, breast cancer risk is significantly reduced in the HIV-infected population. The molecular mechanisms underlying the phenomenon of suppression of breast cancer in the HIV-infected population may serve as a basis for development of a new platform for prevention and treatment of breast cancer. Hypothesis: Various evidences indicate that vasoactive intestinal peptide (VIP) plays an important role in growth, and differentiation of breast cancer. We previously showed (i) that natural antibodies recognizing VIP and the gp120-derived peptide NTM significantly contribute to the control of HIV disease progression by suppression of VIP-like activity of HIV-1 gp120 and (ii) that physical exercise stimulates production of these natural antibodies. These findings suggest that natural anti-VIP/NTM antibodies could contribute to a decrease of breast cancer in the HIV-infected population by suppression of VIP, which may play a pro/oncogenic function. Aerobic exercise which stimulates production of anti-VIP/NTM antibodies could be used as prevention and supportive treatment of breast cancer. Impact: Immunotherapy based on natural anti-VIP/NTM antibodies could serve as an effective adjunct therapy for the treatment of breast cancer. Similarly, aerobic exercise, which stimulates production of these antibodies, should be considered as an inexpensive and safe preventive and supportive breast cancer therapy. Natural anti-VIP/NTM antibodies also represent promising prognostic marker for breast cancer. (C) 2011 Elsevier Ltd. All rights reserved.
T2  - Medical Hypotheses
T1  - Can natural antibodies to VIP or VIP-like HIV-1 glycoprotein facilitate prevention and supportive treatment of breast cancer?
VL  - 77
IS  - 3
SP  - 404
EP  - 408
DO  - 10.1016/j.mehy.2011.05.030
ER  - 

@article{
author = "Veljković, Milena and Branch, Donald R. and Dopsaj, Violeta and Veljković, Veljko and Veljković, Nevena V. and Glišić, Sanja and Colombatti, Alfonso",
year = "2011",
abstract = "Objectives: The incidence of non-AIDS-defining cancer is remarkably higher in HIV-infected than in the general population. In contrast, breast cancer risk is significantly reduced in the HIV-infected population. The molecular mechanisms underlying the phenomenon of suppression of breast cancer in the HIV-infected population may serve as a basis for development of a new platform for prevention and treatment of breast cancer. Hypothesis: Various evidences indicate that vasoactive intestinal peptide (VIP) plays an important role in growth, and differentiation of breast cancer. We previously showed (i) that natural antibodies recognizing VIP and the gp120-derived peptide NTM significantly contribute to the control of HIV disease progression by suppression of VIP-like activity of HIV-1 gp120 and (ii) that physical exercise stimulates production of these natural antibodies. These findings suggest that natural anti-VIP/NTM antibodies could contribute to a decrease of breast cancer in the HIV-infected population by suppression of VIP, which may play a pro/oncogenic function. Aerobic exercise which stimulates production of anti-VIP/NTM antibodies could be used as prevention and supportive treatment of breast cancer. Impact: Immunotherapy based on natural anti-VIP/NTM antibodies could serve as an effective adjunct therapy for the treatment of breast cancer. Similarly, aerobic exercise, which stimulates production of these antibodies, should be considered as an inexpensive and safe preventive and supportive breast cancer therapy. Natural anti-VIP/NTM antibodies also represent promising prognostic marker for breast cancer. (C) 2011 Elsevier Ltd. All rights reserved.",
journal = "Medical Hypotheses",
title = "Can natural antibodies to VIP or VIP-like HIV-1 glycoprotein facilitate prevention and supportive treatment of breast cancer?",
volume = "77",
number = "3",
pages = "404-408",
doi = "10.1016/j.mehy.2011.05.030"
}

Veljković, M., Branch, D. R., Dopsaj, V., Veljković, V., Veljković, N. V., Glišić, S.,& Colombatti, A.. (2011). Can natural antibodies to VIP or VIP-like HIV-1 glycoprotein facilitate prevention and supportive treatment of breast cancer?. in Medical Hypotheses, 77(3), 404-408.
https://doi.org/10.1016/j.mehy.2011.05.030

Veljković M, Branch DR, Dopsaj V, Veljković V, Veljković NV, Glišić S, Colombatti A. Can natural antibodies to VIP or VIP-like HIV-1 glycoprotein facilitate prevention and supportive treatment of breast cancer?. in Medical Hypotheses. 2011;77(3):404-408.
doi:10.1016/j.mehy.2011.05.030 .

Veljković, Milena, Branch, Donald R., Dopsaj, Violeta, Veljković, Veljko, Veljković, Nevena V., Glišić, Sanja, Colombatti, Alfonso, "Can natural antibodies to VIP or VIP-like HIV-1 glycoprotein facilitate prevention and supportive treatment of breast cancer?" in Medical Hypotheses, 77, no. 3 (2011):404-408,
https://doi.org/10.1016/j.mehy.2011.05.030 . .