TY  - JOUR
AU  - Jakovljević, Katarina
AU  - Joksović, Milan D.
AU  - Matić, Ivana Z.
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Sladić, Dušan M.
AU  - Vujčić, Miroslava T.
AU  - Janović, Barbara S.
AU  - Joksović, Ljubinka G.
AU  - Trifunović, Snežana
AU  - Marković, Violeta
PY  - 2018
UR  - http://xlink.rsc.org/?DOI=C8MD00316E
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7928
AB  - Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.
T2  - MedChemComm
T1  - Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies
VL  - 9
IS  - 10
SP  - 1679
EP  - 1697
DO  - 10.1039/C8MD00316E
ER  - 

@article{
author = "Jakovljević, Katarina and Joksović, Milan D. and Matić, Ivana Z. and Petrović, Nina and Stanojković, Tatjana P. and Sladić, Dušan M. and Vujčić, Miroslava T. and Janović, Barbara S. and Joksović, Ljubinka G. and Trifunović, Snežana and Marković, Violeta",
year = "2018",
abstract = "Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.",
journal = "MedChemComm",
title = "Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies",
volume = "9",
number = "10",
pages = "1679-1697",
doi = "10.1039/C8MD00316E"
}

Jakovljević, K., Joksović, M. D., Matić, I. Z., Petrović, N., Stanojković, T. P., Sladić, D. M., Vujčić, M. T., Janović, B. S., Joksović, L. G., Trifunović, S.,& Marković, V.. (2018). Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in MedChemComm, 9(10), 1679-1697.
https://doi.org/10.1039/C8MD00316E

Jakovljević K, Joksović MD, Matić IZ, Petrović N, Stanojković TP, Sladić DM, Vujčić MT, Janović BS, Joksović LG, Trifunović S, Marković V. Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in MedChemComm. 2018;9(10):1679-1697.
doi:10.1039/C8MD00316E .

Jakovljević, Katarina, Joksović, Milan D., Matić, Ivana Z., Petrović, Nina, Stanojković, Tatjana P., Sladić, Dušan M., Vujčić, Miroslava T., Janović, Barbara S., Joksović, Ljubinka G., Trifunović, Snežana, Marković, Violeta, "Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies" in MedChemComm, 9, no. 10 (2018):1679-1697,
https://doi.org/10.1039/C8MD00316E . .

TY  - JOUR
AU  - Filipović, Nenad R.
AU  - Bjelogrlić, Snežana
AU  - Todorović, Tamara R.
AU  - Blagojević, Vladimir A.
AU  - Muller, Christian D.
AU  - Marinković, Aleksandar D.
AU  - Vujčić, Miroslava T.
AU  - Janović, Barbara S.
AU  - Malešević, Aleksandar S.
AU  - Begović, Nebojša
AU  - Senćanski, Milan V.
AU  - Minić, Dragica M.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1331
AB  - A new Ni(II) complex, [Ni(L)(H2O)] (1), with diethyl 3,3-(2,2-(1,1-(pyridine-2,6-diyl) bis(ethan-1-yl-1-ylidene)) bis(hydrazin-1-yl-2-ylidene)) bis(3-oxopropanoate) ligand (H2L) was synthesized as a potential chemotherapeutic agent. Polidentate ligand was coordinated to Ni(II) NNN-tridentately, in dianionic form, while monodentate water coordination completed square-planar geometry around metal. Structure in the solution was determined by NMR spectroscopy and the same coordination mode was observed in the solid state using IR spectroscopy and further verified by DFT calculations and electrochemical studies. Thermal stability of 1 was determined in both air and nitrogen atmosphere. Anticancer activity of 1 was investigated on acute monocytic leukemia (THP-1) and pancreatic adenocarcinoma (AsPC-1) cell lines. On THP-1 cells 1 induced powerful apoptotic response (ED50 = 10 +/- 3 mu M), which was revealed to be only partially caspase-dependent, with activation of caspase-8 as the dominant course. This suggested that experimentally validated covalent binding of 1 to DNA is not the only mechanism responsible for programmed cell death. This was supported with experiments on AsPC-1 cells. Although treatment of those cells with 1 resulted in poor apoptotic response, cell cycle changes showed concentration-dependent shifts indicating a dual mechanism of activity. This study also reviews the results of preliminary biological screening, which demonstrates that 1 displays a unique pattern of anticancer activity with at least two mechanisms involved.
T2  - RSC Advances
T1  - Ni(II) complex with bishydrazone ligand: synthesis, characterization, DNA binding studies and pro-apoptotic and pro-differentiation induction in human cancerous cell lines
VL  - 6
IS  - 110
SP  - 108726
EP  - 108740
DO  - 10.1039/c6ra24604d
ER  - 

@article{
author = "Filipović, Nenad R. and Bjelogrlić, Snežana and Todorović, Tamara R. and Blagojević, Vladimir A. and Muller, Christian D. and Marinković, Aleksandar D. and Vujčić, Miroslava T. and Janović, Barbara S. and Malešević, Aleksandar S. and Begović, Nebojša and Senćanski, Milan V. and Minić, Dragica M.",
year = "2016",
abstract = "A new Ni(II) complex, [Ni(L)(H2O)] (1), with diethyl 3,3-(2,2-(1,1-(pyridine-2,6-diyl) bis(ethan-1-yl-1-ylidene)) bis(hydrazin-1-yl-2-ylidene)) bis(3-oxopropanoate) ligand (H2L) was synthesized as a potential chemotherapeutic agent. Polidentate ligand was coordinated to Ni(II) NNN-tridentately, in dianionic form, while monodentate water coordination completed square-planar geometry around metal. Structure in the solution was determined by NMR spectroscopy and the same coordination mode was observed in the solid state using IR spectroscopy and further verified by DFT calculations and electrochemical studies. Thermal stability of 1 was determined in both air and nitrogen atmosphere. Anticancer activity of 1 was investigated on acute monocytic leukemia (THP-1) and pancreatic adenocarcinoma (AsPC-1) cell lines. On THP-1 cells 1 induced powerful apoptotic response (ED50 = 10 +/- 3 mu M), which was revealed to be only partially caspase-dependent, with activation of caspase-8 as the dominant course. This suggested that experimentally validated covalent binding of 1 to DNA is not the only mechanism responsible for programmed cell death. This was supported with experiments on AsPC-1 cells. Although treatment of those cells with 1 resulted in poor apoptotic response, cell cycle changes showed concentration-dependent shifts indicating a dual mechanism of activity. This study also reviews the results of preliminary biological screening, which demonstrates that 1 displays a unique pattern of anticancer activity with at least two mechanisms involved.",
journal = "RSC Advances",
title = "Ni(II) complex with bishydrazone ligand: synthesis, characterization, DNA binding studies and pro-apoptotic and pro-differentiation induction in human cancerous cell lines",
volume = "6",
number = "110",
pages = "108726-108740",
doi = "10.1039/c6ra24604d"
}

Filipović, N. R., Bjelogrlić, S., Todorović, T. R., Blagojević, V. A., Muller, C. D., Marinković, A. D., Vujčić, M. T., Janović, B. S., Malešević, A. S., Begović, N., Senćanski, M. V.,& Minić, D. M.. (2016). Ni(II) complex with bishydrazone ligand: synthesis, characterization, DNA binding studies and pro-apoptotic and pro-differentiation induction in human cancerous cell lines. in RSC Advances, 6(110), 108726-108740.
https://doi.org/10.1039/c6ra24604d

Filipović NR, Bjelogrlić S, Todorović TR, Blagojević VA, Muller CD, Marinković AD, Vujčić MT, Janović BS, Malešević AS, Begović N, Senćanski MV, Minić DM. Ni(II) complex with bishydrazone ligand: synthesis, characterization, DNA binding studies and pro-apoptotic and pro-differentiation induction in human cancerous cell lines. in RSC Advances. 2016;6(110):108726-108740.
doi:10.1039/c6ra24604d .

Filipović, Nenad R., Bjelogrlić, Snežana, Todorović, Tamara R., Blagojević, Vladimir A., Muller, Christian D., Marinković, Aleksandar D., Vujčić, Miroslava T., Janović, Barbara S., Malešević, Aleksandar S., Begović, Nebojša, Senćanski, Milan V., Minić, Dragica M., "Ni(II) complex with bishydrazone ligand: synthesis, characterization, DNA binding studies and pro-apoptotic and pro-differentiation induction in human cancerous cell lines" in RSC Advances, 6, no. 110 (2016):108726-108740,
https://doi.org/10.1039/c6ra24604d . .

TY  - JOUR
AU  - Vilipić, Jovana P.
AU  - Novaković, Irena T.
AU  - Zlatović, Mario V.
AU  - Vujčić, Miroslava T.
AU  - Tufegdzic, Srđan J.
AU  - Sladić, Dušan M.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1405
AB  - The interactions of nine amino acid derivatives of tert-butylquinone with biomacromolecules were studied. Sodium dodecyl sulphate (SDS) gel electrophoresis and mass spectrometry confirmed the absence of modifications of lysozyme by any of the synthesized compounds. Spectrophotometric studies demonstrated hyperchromism, i.e., the existence of interactions between the quinones and calf thymus DNA (CT-DNA). Determination of the binding constants by absorption titration indicated weak interactions between the quinone derivatives and CT-DNA. The quenching of fluorescence of the intercalator ethidium bromide (EB) from the EB-CT-DNA system and of the minor groove binder Hoechst 33258 (H) from the H-CT-DNA system by the synthesized derivatives indicated interactions of the compounds and CT-DNA. Circular dichroism (CD) spectra demonstrated a non-intercalative binding mode of the quinone derivatives to CT-DNA. Molecular docking results confirmed binding to the minor groove. The electrophoretic pattern showed no cleavage of the pUC19 plasmid in the presence of any of the synthesized compounds. The ability of the derivatives to scavenge radicals was confirmed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. All the presented results suggest that the DNA minor groove binding is the principal mechanism of action of the examined amino acid derivatives.
T2  - Journal of the Serbian Chemical Society
T1  - Interactions of cytotoxic amino acid derivatives of tert-butylquinone with DNA and lysozyme
VL  - 81
IS  - 12
SP  - 1345
EP  - 1358
DO  - 10.2298/JSC160725101V
ER  - 

@article{
author = "Vilipić, Jovana P. and Novaković, Irena T. and Zlatović, Mario V. and Vujčić, Miroslava T. and Tufegdzic, Srđan J. and Sladić, Dušan M.",
year = "2016",
abstract = "The interactions of nine amino acid derivatives of tert-butylquinone with biomacromolecules were studied. Sodium dodecyl sulphate (SDS) gel electrophoresis and mass spectrometry confirmed the absence of modifications of lysozyme by any of the synthesized compounds. Spectrophotometric studies demonstrated hyperchromism, i.e., the existence of interactions between the quinones and calf thymus DNA (CT-DNA). Determination of the binding constants by absorption titration indicated weak interactions between the quinone derivatives and CT-DNA. The quenching of fluorescence of the intercalator ethidium bromide (EB) from the EB-CT-DNA system and of the minor groove binder Hoechst 33258 (H) from the H-CT-DNA system by the synthesized derivatives indicated interactions of the compounds and CT-DNA. Circular dichroism (CD) spectra demonstrated a non-intercalative binding mode of the quinone derivatives to CT-DNA. Molecular docking results confirmed binding to the minor groove. The electrophoretic pattern showed no cleavage of the pUC19 plasmid in the presence of any of the synthesized compounds. The ability of the derivatives to scavenge radicals was confirmed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. All the presented results suggest that the DNA minor groove binding is the principal mechanism of action of the examined amino acid derivatives.",
journal = "Journal of the Serbian Chemical Society",
title = "Interactions of cytotoxic amino acid derivatives of tert-butylquinone with DNA and lysozyme",
volume = "81",
number = "12",
pages = "1345-1358",
doi = "10.2298/JSC160725101V"
}

Vilipić, J. P., Novaković, I. T., Zlatović, M. V., Vujčić, M. T., Tufegdzic, S. J.,& Sladić, D. M.. (2016). Interactions of cytotoxic amino acid derivatives of tert-butylquinone with DNA and lysozyme. in Journal of the Serbian Chemical Society, 81(12), 1345-1358.
https://doi.org/10.2298/JSC160725101V

Vilipić JP, Novaković IT, Zlatović MV, Vujčić MT, Tufegdzic SJ, Sladić DM. Interactions of cytotoxic amino acid derivatives of tert-butylquinone with DNA and lysozyme. in Journal of the Serbian Chemical Society. 2016;81(12):1345-1358.
doi:10.2298/JSC160725101V .

Vilipić, Jovana P., Novaković, Irena T., Zlatović, Mario V., Vujčić, Miroslava T., Tufegdzic, Srđan J., Sladić, Dušan M., "Interactions of cytotoxic amino acid derivatives of tert-butylquinone with DNA and lysozyme" in Journal of the Serbian Chemical Society, 81, no. 12 (2016):1345-1358,
https://doi.org/10.2298/JSC160725101V . .

TY  - JOUR
AU  - Filipović, Nenad R.
AU  - Bjelogrlić, Snežana
AU  - Marinković, Aleksandar D.
AU  - Verbić, Tatjana Z.
AU  - Cvijetić, Ilija N.
AU  - Senćanski, Milan V.
AU  - Rodić, Marko
AU  - Vujčić, Miroslava T.
AU  - Sladić, Dušan M.
AU  - Striković, Zlatko
AU  - Todorović, Tamara R.
AU  - Muller, Christian D.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/809
AB  - A new Zn(II)-based potential chemotherapeutic agent was synthesized from the ligand 2-quinolinecarboxaldehyde selenosemicarbazone (Hqasesc). Single crystal X-ray diffraction analysis showed that the Zn(II) complex consists of a cation [Zn(Hqasesc)(2)](2+), two perchlorate anions and one ethanol solvent molecule. The interaction of calf thymus (CT) DNA and human serum albumin (HSA) with the Zn(II) complex was explored using absorption and emission spectral methods, and also has been supported by molecular docking studies. The complex has more affinity to minor DNA groove than major, with no significant intercalation. The HSA interaction studies of the complex revealed the quenching of the intrinsic fluorescence of the HSA through a static quenching mechanism. The antitumor activity of the ligand and the complex against pancreatic adenocarcinoma cell line (AsPC-1) and acute monocytic leukemia (THP-1) cells was evaluated. Both compounds are strong concentration-dependent apoptosis inducers in THP-1 cells. While Hqasesc in AsPC-1 cells induces apoptosis only at the highest concentration, treatment with the Zn complex shows a concentration-dependent apoptotic response, where the treated cells are arrested in the G1-to-S phase accompanied with extensive activation of caspase-8 and -9. These results indicate that the ligand and Zn(II) complex display cell phenotype specific activity.
T2  - RSC Advances
T1  - Zn(II) complex with 2-quinolinecarboxaldehyde selenosemicarbazone: synthesis, structure, interaction studies with DNA/HSA, molecular docking and caspase-8 and-9 independent apoptose induction
VL  - 5
IS  - 115
SP  - 95191
EP  - 95211
DO  - 10.1039/c5ra19849f
ER  - 

@article{
author = "Filipović, Nenad R. and Bjelogrlić, Snežana and Marinković, Aleksandar D. and Verbić, Tatjana Z. and Cvijetić, Ilija N. and Senćanski, Milan V. and Rodić, Marko and Vujčić, Miroslava T. and Sladić, Dušan M. and Striković, Zlatko and Todorović, Tamara R. and Muller, Christian D.",
year = "2015",
abstract = "A new Zn(II)-based potential chemotherapeutic agent was synthesized from the ligand 2-quinolinecarboxaldehyde selenosemicarbazone (Hqasesc). Single crystal X-ray diffraction analysis showed that the Zn(II) complex consists of a cation [Zn(Hqasesc)(2)](2+), two perchlorate anions and one ethanol solvent molecule. The interaction of calf thymus (CT) DNA and human serum albumin (HSA) with the Zn(II) complex was explored using absorption and emission spectral methods, and also has been supported by molecular docking studies. The complex has more affinity to minor DNA groove than major, with no significant intercalation. The HSA interaction studies of the complex revealed the quenching of the intrinsic fluorescence of the HSA through a static quenching mechanism. The antitumor activity of the ligand and the complex against pancreatic adenocarcinoma cell line (AsPC-1) and acute monocytic leukemia (THP-1) cells was evaluated. Both compounds are strong concentration-dependent apoptosis inducers in THP-1 cells. While Hqasesc in AsPC-1 cells induces apoptosis only at the highest concentration, treatment with the Zn complex shows a concentration-dependent apoptotic response, where the treated cells are arrested in the G1-to-S phase accompanied with extensive activation of caspase-8 and -9. These results indicate that the ligand and Zn(II) complex display cell phenotype specific activity.",
journal = "RSC Advances",
title = "Zn(II) complex with 2-quinolinecarboxaldehyde selenosemicarbazone: synthesis, structure, interaction studies with DNA/HSA, molecular docking and caspase-8 and-9 independent apoptose induction",
volume = "5",
number = "115",
pages = "95191-95211",
doi = "10.1039/c5ra19849f"
}

Filipović, N. R., Bjelogrlić, S., Marinković, A. D., Verbić, T. Z., Cvijetić, I. N., Senćanski, M. V., Rodić, M., Vujčić, M. T., Sladić, D. M., Striković, Z., Todorović, T. R.,& Muller, C. D.. (2015). Zn(II) complex with 2-quinolinecarboxaldehyde selenosemicarbazone: synthesis, structure, interaction studies with DNA/HSA, molecular docking and caspase-8 and-9 independent apoptose induction. in RSC Advances, 5(115), 95191-95211.
https://doi.org/10.1039/c5ra19849f

Filipović NR, Bjelogrlić S, Marinković AD, Verbić TZ, Cvijetić IN, Senćanski MV, Rodić M, Vujčić MT, Sladić DM, Striković Z, Todorović TR, Muller CD. Zn(II) complex with 2-quinolinecarboxaldehyde selenosemicarbazone: synthesis, structure, interaction studies with DNA/HSA, molecular docking and caspase-8 and-9 independent apoptose induction. in RSC Advances. 2015;5(115):95191-95211.
doi:10.1039/c5ra19849f .

Filipović, Nenad R., Bjelogrlić, Snežana, Marinković, Aleksandar D., Verbić, Tatjana Z., Cvijetić, Ilija N., Senćanski, Milan V., Rodić, Marko, Vujčić, Miroslava T., Sladić, Dušan M., Striković, Zlatko, Todorović, Tamara R., Muller, Christian D., "Zn(II) complex with 2-quinolinecarboxaldehyde selenosemicarbazone: synthesis, structure, interaction studies with DNA/HSA, molecular docking and caspase-8 and-9 independent apoptose induction" in RSC Advances, 5, no. 115 (2015):95191-95211,
https://doi.org/10.1039/c5ra19849f . .

TY  - JOUR
AU  - Vujčić, Miroslava T.
AU  - Velickovic, Natasa
AU  - Ruždijić, Sabera
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3326
AB  - Aging is associated with marked changes in the biochemical processes of many organs. Basal and glucocorticoid induced of liver nuclear glucocorticoid receptor (GR) on the level of protein expression and DNA-binding activity were investigated at different ages (3, 6, 12, 18 and 24 months old) in two groups of rats in: untreated and dexamethasone treated. The results showed a significant decline of GR protein immunopurified from untreated rats of advanced age. In dexamethasone-treated rats, the quantity of GR protein was lower than in controls at all ages. The interactions of liver nuclear proteins with radioactively labelled synthetic oligonucleotide analogue containing consensus GRE sequence were analysed during aging. The results showed that GRE binding activity demonstrated a decrease both in untreated and in dexamethasone treated rats. However, relative to untreated rats, dexamethasone treatment resulted in a significant increase in GRE binding at all ages, except that of three months old animals. In conclusion, the observed alterations in GR protein expression and its DNA binding activity may play a role in the changes of the cell response to glucocorticoid during aging. (c) 2007 Elsevier Inc. All rights reserved.
T2  - Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology
T1  - Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging
VL  - 148
IS  - 4
SP  - 463
EP  - 469
DO  - 10.1016/j.cbpb.2007.07.090
ER  - 

@article{
author = "Vujčić, Miroslava T. and Velickovic, Natasa and Ruždijić, Sabera",
year = "2007",
abstract = "Aging is associated with marked changes in the biochemical processes of many organs. Basal and glucocorticoid induced of liver nuclear glucocorticoid receptor (GR) on the level of protein expression and DNA-binding activity were investigated at different ages (3, 6, 12, 18 and 24 months old) in two groups of rats in: untreated and dexamethasone treated. The results showed a significant decline of GR protein immunopurified from untreated rats of advanced age. In dexamethasone-treated rats, the quantity of GR protein was lower than in controls at all ages. The interactions of liver nuclear proteins with radioactively labelled synthetic oligonucleotide analogue containing consensus GRE sequence were analysed during aging. The results showed that GRE binding activity demonstrated a decrease both in untreated and in dexamethasone treated rats. However, relative to untreated rats, dexamethasone treatment resulted in a significant increase in GRE binding at all ages, except that of three months old animals. In conclusion, the observed alterations in GR protein expression and its DNA binding activity may play a role in the changes of the cell response to glucocorticoid during aging. (c) 2007 Elsevier Inc. All rights reserved.",
journal = "Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology",
title = "Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging",
volume = "148",
number = "4",
pages = "463-469",
doi = "10.1016/j.cbpb.2007.07.090"
}

Vujčić, M. T., Velickovic, N.,& Ruždijić, S.. (2007). Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging. in Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology, 148(4), 463-469.
https://doi.org/10.1016/j.cbpb.2007.07.090

Vujčić MT, Velickovic N, Ruždijić S. Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging. in Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology. 2007;148(4):463-469.
doi:10.1016/j.cbpb.2007.07.090 .

Vujčić, Miroslava T., Velickovic, Natasa, Ruždijić, Sabera, "Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging" in Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology, 148, no. 4 (2007):463-469,
https://doi.org/10.1016/j.cbpb.2007.07.090 . .

TY  - JOUR
AU  - Vujčić, Miroslava T.
AU  - Terzić, N
AU  - Ristić-Fira, Aleksandra
AU  - Kanazir, Selma
AU  - Ruždijić, Sabera
PY  - 2005
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2906
AB  - In order to contribute to the understanding of mechanisms by which regulatory proteins recognize genetic information stored in DNA, analyses of their interaction with specific nucleotides are usually performed. In this study, the electrophoretic mobility shift assay (EMSA) was applied to analyze the interaction of nuclear proteins from the liver of rats of different age i.e., young (3-month-old), middle-aged (12-month-old) and aged (24-month-old), with radioactively labelled synthetic oligonucleotide analogues, corresponding to GRE. The levels of GRE binding activity were assessed by quantitative densitometric scanning of the autoradiograms. The results showed statistically significant decreasing values of up to 78% and 49% in middle aged and old animals, respectively, compared to young animals (p LT 0.05). The specificity of the nuclear proteins-GRE interaction was demonstrated by competition experiments with unlabelled GRE. In a supershift assay, using the antibody BuGR2, it was shown that the GR proteins present in nuclear extracts have a high affinity for the GRE probe. The stabilities of the protein-DNA complexes were analysed and it was concluded that they changed during ageing.
T2  - Journal of the Serbian Chemical Society
T1  - Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver
VL  - 70
IS  - 5
SP  - 705
EP  - 712
DO  - 10.2298/JSC0505705V
ER  - 

@article{
author = "Vujčić, Miroslava T. and Terzić, N and Ristić-Fira, Aleksandra and Kanazir, Selma and Ruždijić, Sabera",
year = "2005",
abstract = "In order to contribute to the understanding of mechanisms by which regulatory proteins recognize genetic information stored in DNA, analyses of their interaction with specific nucleotides are usually performed. In this study, the electrophoretic mobility shift assay (EMSA) was applied to analyze the interaction of nuclear proteins from the liver of rats of different age i.e., young (3-month-old), middle-aged (12-month-old) and aged (24-month-old), with radioactively labelled synthetic oligonucleotide analogues, corresponding to GRE. The levels of GRE binding activity were assessed by quantitative densitometric scanning of the autoradiograms. The results showed statistically significant decreasing values of up to 78% and 49% in middle aged and old animals, respectively, compared to young animals (p LT 0.05). The specificity of the nuclear proteins-GRE interaction was demonstrated by competition experiments with unlabelled GRE. In a supershift assay, using the antibody BuGR2, it was shown that the GR proteins present in nuclear extracts have a high affinity for the GRE probe. The stabilities of the protein-DNA complexes were analysed and it was concluded that they changed during ageing.",
journal = "Journal of the Serbian Chemical Society",
title = "Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver",
volume = "70",
number = "5",
pages = "705-712",
doi = "10.2298/JSC0505705V"
}

Vujčić, M. T., Terzić, N., Ristić-Fira, A., Kanazir, S.,& Ruždijić, S.. (2005). Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver. in Journal of the Serbian Chemical Society, 70(5), 705-712.
https://doi.org/10.2298/JSC0505705V

Vujčić MT, Terzić N, Ristić-Fira A, Kanazir S, Ruždijić S. Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver. in Journal of the Serbian Chemical Society. 2005;70(5):705-712.
doi:10.2298/JSC0505705V .

Vujčić, Miroslava T., Terzić, N, Ristić-Fira, Aleksandra, Kanazir, Selma, Ruždijić, Sabera, "Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver" in Journal of the Serbian Chemical Society, 70, no. 5 (2005):705-712,
https://doi.org/10.2298/JSC0505705V . .

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
AU  - Todorović, Danijela V.
AU  - Korićanac, Lela
AU  - Vujčić, Miroslava T.
AU  - Demajo, Miroslav
AU  - Sabini, G
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2829
AB  - The effects of single irradiation with gamma rays and protons on HTB63 human melanoma cell growth were compared. The exponentially growing cells were irradiated with gamma rays or protons using doses ranging from 2-20 Gy. At 48 It of post-irradiation incubation under standard conditions, cell survival and induction of apoptotic cell death were examined. The best effect of the single irradiation with gamma rays was the reduction of cell growth by up to 26% (p=0.048, irradiation vs. control), obtained using the dose of 16 Gy. The same doses of proton irradiation, having energy at the target of 22.6 MeV, significantly inhibited melanoma cell growth. Doses of 12 and 16 Gy of protons provoked growth inhibition of 48.9% (p=0.003, irradiation vs. control) and 51.2% (p=0.012, irradiation vs. control) respectively. Irradiation with 12 and 16 Gy protons, compared to the effects of the same doses of gamma rays, significantly reduced melanoma cell growth (p=0.015 and p=0.028, protons vs. gamma rays, respectively). Estimated RBEs for growth inhibition of HTB63 cells ranged from 1.02 to 1.45. The electrophoretical analyses of DNA samples and flow cytometric evaluation have shown a low percentage of apoptotic cells after both types of irradiation. The better inhibitory effect achieved by protons in contrast to gamma rays, can be explained considering specific physical properties of protons, especially taking into account the highly localized energy deposition (high LET).
T2  - Oncology Reports
T1  - Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays
VL  - 12
IS  - 6
SP  - 1323
EP  - 1328
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2829
ER  - 

@article{
author = "Ristić-Fira, Aleksandra and Petrović, Ivan M. and Todorović, Danijela V. and Korićanac, Lela and Vujčić, Miroslava T. and Demajo, Miroslav and Sabini, G and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo",
year = "2004",
abstract = "The effects of single irradiation with gamma rays and protons on HTB63 human melanoma cell growth were compared. The exponentially growing cells were irradiated with gamma rays or protons using doses ranging from 2-20 Gy. At 48 It of post-irradiation incubation under standard conditions, cell survival and induction of apoptotic cell death were examined. The best effect of the single irradiation with gamma rays was the reduction of cell growth by up to 26% (p=0.048, irradiation vs. control), obtained using the dose of 16 Gy. The same doses of proton irradiation, having energy at the target of 22.6 MeV, significantly inhibited melanoma cell growth. Doses of 12 and 16 Gy of protons provoked growth inhibition of 48.9% (p=0.003, irradiation vs. control) and 51.2% (p=0.012, irradiation vs. control) respectively. Irradiation with 12 and 16 Gy protons, compared to the effects of the same doses of gamma rays, significantly reduced melanoma cell growth (p=0.015 and p=0.028, protons vs. gamma rays, respectively). Estimated RBEs for growth inhibition of HTB63 cells ranged from 1.02 to 1.45. The electrophoretical analyses of DNA samples and flow cytometric evaluation have shown a low percentage of apoptotic cells after both types of irradiation. The better inhibitory effect achieved by protons in contrast to gamma rays, can be explained considering specific physical properties of protons, especially taking into account the highly localized energy deposition (high LET).",
journal = "Oncology Reports",
title = "Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays",
volume = "12",
number = "6",
pages = "1323-1328",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2829"
}

Ristić-Fira, A., Petrović, I. M., Todorović, D. V., Korićanac, L., Vujčić, M. T., Demajo, M., Sabini, G., Cirrone, G. A. P.,& Cuttone, G.. (2004). Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays. in Oncology Reports, 12(6), 1323-1328.
https://hdl.handle.net/21.15107/rcub_vinar_2829

Ristić-Fira A, Petrović IM, Todorović DV, Korićanac L, Vujčić MT, Demajo M, Sabini G, Cirrone GAP, Cuttone G. Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays. in Oncology Reports. 2004;12(6):1323-1328.
https://hdl.handle.net/21.15107/rcub_vinar_2829 .

Ristić-Fira, Aleksandra, Petrović, Ivan M., Todorović, Danijela V., Korićanac, Lela, Vujčić, Miroslava T., Demajo, Miroslav, Sabini, G, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays" in Oncology Reports, 12, no. 6 (2004):1323-1328,
https://hdl.handle.net/21.15107/rcub_vinar_2829 .

TY  - CONF
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Vujčić, Miroslava T.
AU  - Korićanac, Lela
AU  - Ruždijić, Sabera
AU  - Demajo, Miroslav
AU  - Cuttone, Giacomo
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8631
C3  - Turkish Journal of Biochemistry
T1  - Inactivation of melanoma cells irradiated with gamma rays and low energy protons
VL  - 28
IS  - 3
SP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_vinar_8631
ER  - 

@conference{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V. and Vujčić, Miroslava T. and Korićanac, Lela and Ruždijić, Sabera and Demajo, Miroslav and Cuttone, Giacomo",
year = "2003",
journal = "Turkish Journal of Biochemistry",
title = "Inactivation of melanoma cells irradiated with gamma rays and low energy protons",
volume = "28",
number = "3",
pages = "85",
url = "https://hdl.handle.net/21.15107/rcub_vinar_8631"
}

Petrović, I. M., Ristić-Fira, A., Todorović, D. V., Vujčić, M. T., Korićanac, L., Ruždijić, S., Demajo, M.,& Cuttone, G.. (2003). Inactivation of melanoma cells irradiated with gamma rays and low energy protons. in Turkish Journal of Biochemistry, 28(3), 85.
https://hdl.handle.net/21.15107/rcub_vinar_8631

Petrović IM, Ristić-Fira A, Todorović DV, Vujčić MT, Korićanac L, Ruždijić S, Demajo M, Cuttone G. Inactivation of melanoma cells irradiated with gamma rays and low energy protons. in Turkish Journal of Biochemistry. 2003;28(3):85.
https://hdl.handle.net/21.15107/rcub_vinar_8631 .

Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., Vujčić, Miroslava T., Korićanac, Lela, Ruždijić, Sabera, Demajo, Miroslav, Cuttone, Giacomo, "Inactivation of melanoma cells irradiated with gamma rays and low energy protons" in Turkish Journal of Biochemistry, 28, no. 3 (2003):85,
https://hdl.handle.net/21.15107/rcub_vinar_8631 .

TY  - JOUR
AU  - Terzić, N
AU  - Vujčić, Miroslava T.
AU  - Ristić-Fira, Aleksandra
AU  - Krstić-Demonacos, Marija
AU  - Milanovic, D
AU  - Kanazir, Dušan T.
AU  - Ruždijić, Sabera
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2623
AB  - The effect of dexamethasone (DEX) on glucocorticoid receptor (GR)-mediated gene expression was examined in the brain of young and aged rats. Electrophoretic mobility shift assays showed that DEX treatment led to an increase of glucocorticoid response element (GRE) binding activity in aged rats, whereas in young animals GRE binding activity was decreased. Western blot analysis and reverse transcriptase polymerase chain reaction confirmed that, in aged animals, the GR mRNA and the GR protein levels were increased on DEX treatment. The binding activity of GRE activating protein-1 (AP-1) site and cross-competition analysis demonstrated specific pattern of expression during the ageing and DEX treatment, suggesting that GR modulates the activity of transcription factors AP-1 (Fos/Jun proteins) through protein-protein interaction. On the basis of these results, it can be concluded that the composition of transcriptional complexes that bind to GRE and AP-I regulatory elements changes upon DEX treatment in an age-specific manner.
T2  - Journals of Gerontology. Series A: Biological Sciences and Medical Sciences
T1  - Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain
VL  - 58
IS  - 4
SP  - 297
EP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2623
ER  - 

@article{
author = "Terzić, N and Vujčić, Miroslava T. and Ristić-Fira, Aleksandra and Krstić-Demonacos, Marija and Milanovic, D and Kanazir, Dušan T. and Ruždijić, Sabera",
year = "2003",
abstract = "The effect of dexamethasone (DEX) on glucocorticoid receptor (GR)-mediated gene expression was examined in the brain of young and aged rats. Electrophoretic mobility shift assays showed that DEX treatment led to an increase of glucocorticoid response element (GRE) binding activity in aged rats, whereas in young animals GRE binding activity was decreased. Western blot analysis and reverse transcriptase polymerase chain reaction confirmed that, in aged animals, the GR mRNA and the GR protein levels were increased on DEX treatment. The binding activity of GRE activating protein-1 (AP-1) site and cross-competition analysis demonstrated specific pattern of expression during the ageing and DEX treatment, suggesting that GR modulates the activity of transcription factors AP-1 (Fos/Jun proteins) through protein-protein interaction. On the basis of these results, it can be concluded that the composition of transcriptional complexes that bind to GRE and AP-I regulatory elements changes upon DEX treatment in an age-specific manner.",
journal = "Journals of Gerontology. Series A: Biological Sciences and Medical Sciences",
title = "Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain",
volume = "58",
number = "4",
pages = "297-303",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2623"
}

Terzić, N., Vujčić, M. T., Ristić-Fira, A., Krstić-Demonacos, M., Milanovic, D., Kanazir, D. T.,& Ruždijić, S.. (2003). Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain. in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences, 58(4), 297-303.
https://hdl.handle.net/21.15107/rcub_vinar_2623

Terzić N, Vujčić MT, Ristić-Fira A, Krstić-Demonacos M, Milanovic D, Kanazir DT, Ruždijić S. Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain. in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences. 2003;58(4):297-303.
https://hdl.handle.net/21.15107/rcub_vinar_2623 .

Terzić, N, Vujčić, Miroslava T., Ristić-Fira, Aleksandra, Krstić-Demonacos, Marija, Milanovic, D, Kanazir, Dušan T., Ruždijić, Sabera, "Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain" in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences, 58, no. 4 (2003):297-303,
https://hdl.handle.net/21.15107/rcub_vinar_2623 .

TY  - JOUR
AU  - Vujčić, Miroslava T.
AU  - Đorđević Marković, R.
AU  - Radić, Olivera
AU  - Krstić, M.
AU  - Kanazir, Dušan T.
PY  - 1996
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2030
T2  - Jugoslovenska Medicinska Biohemija
T1  - Analysis of kinase activity associated with corticosteroid binder IB
VL  - 15
IS  - 4
SP  - 319
EP  - 320
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2030
ER  - 

@article{
author = "Vujčić, Miroslava T. and Đorđević Marković, R. and Radić, Olivera and Krstić, M. and Kanazir, Dušan T.",
year = "1996",
journal = "Jugoslovenska Medicinska Biohemija",
title = "Analysis of kinase activity associated with corticosteroid binder IB",
volume = "15",
number = "4",
pages = "319-320",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2030"
}

Vujčić, M. T., Đorđević Marković, R., Radić, O., Krstić, M.,& Kanazir, D. T.. (1996). Analysis of kinase activity associated with corticosteroid binder IB. in Jugoslovenska Medicinska Biohemija, 15(4), 319-320.
https://hdl.handle.net/21.15107/rcub_vinar_2030

Vujčić MT, Đorđević Marković R, Radić O, Krstić M, Kanazir DT. Analysis of kinase activity associated with corticosteroid binder IB. in Jugoslovenska Medicinska Biohemija. 1996;15(4):319-320.
https://hdl.handle.net/21.15107/rcub_vinar_2030 .

Vujčić, Miroslava T., Đorđević Marković, R., Radić, Olivera, Krstić, M., Kanazir, Dušan T., "Analysis of kinase activity associated with corticosteroid binder IB" in Jugoslovenska Medicinska Biohemija, 15, no. 4 (1996):319-320,
https://hdl.handle.net/21.15107/rcub_vinar_2030 .