TY  - JOUR
AU  - Stojanovic, Igor Z.
AU  - Stanković, Miroslava
AU  - Jovanovic, Olga
AU  - Petrovic, Goran
AU  - Smelcerovic, Andrija
AU  - Stojanović, Gordana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5302
AB  - Three lichen depsidones, physodalic acid (1), physodic acid (2), and 3-hydroxy physodic acid (3), were isolated from Hypogymnia physodes diethyl ether extract using column chromatography, and their structures determined by comparing their UV, H-1 and C-13 NMR spectroscopic and MS data with those given in the literature, as well as with data computed by CHEM draw ultra 11 software. The contents of 1, 2 and 3 were determined in the methanol (ME), acetone (AE), and diethyl ether (EE) extracts using reversed-phase high performance liquid chromatography with photodiode array detection. The extracts, isolated depsidones 1-3 and EE fraction F23 (consisting of 90% 2 and 3, in the ratio 5.5: 1) were evaluated for their in vitro effects on chromosome aberrations in peripheral human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay in doses of 1 mu g/mL and 2 mu g/mL of final culture solution. The frequency of MN was scored in binucleated cells, and nuclear proliferation index was calculated. It was found that 1, 2, 3, F23, and EE at 1.0 mu g/mL exerted a beneficial effect on lymphocyte cells giving a significant decrease of the frequency of MN in comparison with the positive control Amifostin WR-2721. Among the tested depsidones at a concentration of 1 mu g/mL, 3 exhibited the most prominent effect decreasing the frequency of MN by 30.3%, followed by 2 (28.2%) and 1 (22.0%). The extracts were less effective than the isolated depsidones.
T2  - Natural Product Communications
T1  - Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes
VL  - 8
IS  - 1
SP  - 109
EP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5302
ER  - 

@article{
author = "Stojanovic, Igor Z. and Stanković, Miroslava and Jovanovic, Olga and Petrovic, Goran and Smelcerovic, Andrija and Stojanović, Gordana",
year = "2013",
abstract = "Three lichen depsidones, physodalic acid (1), physodic acid (2), and 3-hydroxy physodic acid (3), were isolated from Hypogymnia physodes diethyl ether extract using column chromatography, and their structures determined by comparing their UV, H-1 and C-13 NMR spectroscopic and MS data with those given in the literature, as well as with data computed by CHEM draw ultra 11 software. The contents of 1, 2 and 3 were determined in the methanol (ME), acetone (AE), and diethyl ether (EE) extracts using reversed-phase high performance liquid chromatography with photodiode array detection. The extracts, isolated depsidones 1-3 and EE fraction F23 (consisting of 90% 2 and 3, in the ratio 5.5: 1) were evaluated for their in vitro effects on chromosome aberrations in peripheral human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay in doses of 1 mu g/mL and 2 mu g/mL of final culture solution. The frequency of MN was scored in binucleated cells, and nuclear proliferation index was calculated. It was found that 1, 2, 3, F23, and EE at 1.0 mu g/mL exerted a beneficial effect on lymphocyte cells giving a significant decrease of the frequency of MN in comparison with the positive control Amifostin WR-2721. Among the tested depsidones at a concentration of 1 mu g/mL, 3 exhibited the most prominent effect decreasing the frequency of MN by 30.3%, followed by 2 (28.2%) and 1 (22.0%). The extracts were less effective than the isolated depsidones.",
journal = "Natural Product Communications",
title = "Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes",
volume = "8",
number = "1",
pages = "109-112",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5302"
}

Stojanovic, I. Z., Stanković, M., Jovanovic, O., Petrovic, G., Smelcerovic, A.,& Stojanović, G.. (2013). Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes. in Natural Product Communications, 8(1), 109-112.
https://hdl.handle.net/21.15107/rcub_vinar_5302

Stojanovic IZ, Stanković M, Jovanovic O, Petrovic G, Smelcerovic A, Stojanović G. Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes. in Natural Product Communications. 2013;8(1):109-112.
https://hdl.handle.net/21.15107/rcub_vinar_5302 .

Stojanovic, Igor Z., Stanković, Miroslava, Jovanovic, Olga, Petrovic, Goran, Smelcerovic, Andrija, Stojanović, Gordana, "Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes" in Natural Product Communications, 8, no. 1 (2013):109-112,
https://hdl.handle.net/21.15107/rcub_vinar_5302 .