TY  - CONF
AU  - Čolović, Mirjana
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Savić, Nada
AU  - Parac-Vogt, Tatjana
AU  - Krstić, Danijela
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12944
AB  - The aim of this study was to in vitro assess the anti-tumor potential of Fe(III)-substituted monolacunary Wells-Dawson polyoxotungstate, K7[FeIII(α2-P2W17O61)(H2O)] (FeWD) against human melanoma A375 cells. A375 cells were treated in vitro with FeWD in the concentration range of 0.001-1 mM, for 24, 48, and 72 hours. FeWD decreased A375 cell viability in a dose- and time-dependent manner. IC50 values (in mM), as a marker of the cytotoxic potency of FeWD, were obtained as follows: 1, 0.58, and 0.51, for 24-, 48-, and 72-hour exposure, respectively. However, in comparison with cisplatin as a gold standard in cancer chemotherapy, which was used as a positive control, IC50 values (in mM) were significantly higher than those obtained for cisplatin (0.09, 0.07, and 0.043, for 24, 48, and 72 hours, respectively). For this reason, the studied FeWD polyoxometalate could not be considered a superior anti-cancer candidate compared to the standard chemotherapeutic.
C3  - International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings
T1  - Anti-human melanoma effect of Fe(III)-containing wells-dawson nanocluster in vitro
VL  - 2
SP  - 24
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12944
ER  - 

@conference{
author = "Čolović, Mirjana and Korićanac, Lela and Žakula, Jelena and Savić, Nada and Parac-Vogt, Tatjana and Krstić, Danijela",
year = "2024",
abstract = "The aim of this study was to in vitro assess the anti-tumor potential of Fe(III)-substituted monolacunary Wells-Dawson polyoxotungstate, K7[FeIII(α2-P2W17O61)(H2O)] (FeWD) against human melanoma A375 cells. A375 cells were treated in vitro with FeWD in the concentration range of 0.001-1 mM, for 24, 48, and 72 hours. FeWD decreased A375 cell viability in a dose- and time-dependent manner. IC50 values (in mM), as a marker of the cytotoxic potency of FeWD, were obtained as follows: 1, 0.58, and 0.51, for 24-, 48-, and 72-hour exposure, respectively. However, in comparison with cisplatin as a gold standard in cancer chemotherapy, which was used as a positive control, IC50 values (in mM) were significantly higher than those obtained for cisplatin (0.09, 0.07, and 0.043, for 24, 48, and 72 hours, respectively). For this reason, the studied FeWD polyoxometalate could not be considered a superior anti-cancer candidate compared to the standard chemotherapeutic.",
journal = "International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings",
title = "Anti-human melanoma effect of Fe(III)-containing wells-dawson nanocluster in vitro",
volume = "2",
pages = "24-27",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12944"
}

Čolović, M., Korićanac, L., Žakula, J., Savić, N., Parac-Vogt, T.,& Krstić, D.. (2024). Anti-human melanoma effect of Fe(III)-containing wells-dawson nanocluster in vitro. in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings, 2, 24-27.
https://hdl.handle.net/21.15107/rcub_vinar_12944

Čolović M, Korićanac L, Žakula J, Savić N, Parac-Vogt T, Krstić D. Anti-human melanoma effect of Fe(III)-containing wells-dawson nanocluster in vitro. in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings. 2024;2:24-27.
https://hdl.handle.net/21.15107/rcub_vinar_12944 .

Čolović, Mirjana, Korićanac, Lela, Žakula, Jelena, Savić, Nada, Parac-Vogt, Tatjana, Krstić, Danijela, "Anti-human melanoma effect of Fe(III)-containing wells-dawson nanocluster in vitro" in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings, 2 (2024):24-27,
https://hdl.handle.net/21.15107/rcub_vinar_12944 .

TY  - CONF
AU  - Krstić, Danijela
AU  - Lalatović, Jovana
AU  - Mrđa, Davor
AU  - Stojanović, Marko
AU  - Savić, Nada
AU  - Parac-Vogt, Tatjana
AU  - Čolović, Mirjana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12945
AB  - The purpose of this study was to evaluate in vitro contrast properties of a synthesized Wells-Dawson P2W17O61)2]·19H2O (Hf-WD 1:2), as a potential contrast agent for computed tomography (CT). X-ray attenuation was determined in vitro in the presence of increasing tungsten concentrations (3.125-100 mM) of Hf-WD 1:2. In order to compare the contrast properties of the tungsten-containing Hf-WD 1:2 with a commercially available CT contrast agent that has been used in clinical practice, X-ray attenuation was presented for the same iodine concentrations of iodine-based iohexol solution as well. Higher HU values for particular concentrations were obtained for Hf-WD 1:2 in comparison with those determined for the standard iohexol. Thus, the studied Hf-WD 1:2 POM could be considered a promising contrast agent candidate for CT. Nevertheless, further development of Hf-WD 1:2 as a potential CT contrast requires additional CT studies in vivo and relevant toxicity studies in vitro and in vivo as well.
C3  - International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings
T1  - Hafnium-containing Wells-Dawson nanocluster as a promising contrast agent candidate
VL  - 2
SP  - 39
EP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12945
ER  - 

@conference{
author = "Krstić, Danijela and Lalatović, Jovana and Mrđa, Davor and Stojanović, Marko and Savić, Nada and Parac-Vogt, Tatjana and Čolović, Mirjana",
year = "2024",
abstract = "The purpose of this study was to evaluate in vitro contrast properties of a synthesized Wells-Dawson P2W17O61)2]·19H2O (Hf-WD 1:2), as a potential contrast agent for computed tomography (CT). X-ray attenuation was determined in vitro in the presence of increasing tungsten concentrations (3.125-100 mM) of Hf-WD 1:2. In order to compare the contrast properties of the tungsten-containing Hf-WD 1:2 with a commercially available CT contrast agent that has been used in clinical practice, X-ray attenuation was presented for the same iodine concentrations of iodine-based iohexol solution as well. Higher HU values for particular concentrations were obtained for Hf-WD 1:2 in comparison with those determined for the standard iohexol. Thus, the studied Hf-WD 1:2 POM could be considered a promising contrast agent candidate for CT. Nevertheless, further development of Hf-WD 1:2 as a potential CT contrast requires additional CT studies in vivo and relevant toxicity studies in vitro and in vivo as well.",
journal = "International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings",
title = "Hafnium-containing Wells-Dawson nanocluster as a promising contrast agent candidate",
volume = "2",
pages = "39-42",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12945"
}

Krstić, D., Lalatović, J., Mrđa, D., Stojanović, M., Savić, N., Parac-Vogt, T.,& Čolović, M.. (2024). Hafnium-containing Wells-Dawson nanocluster as a promising contrast agent candidate. in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings, 2, 39-42.
https://hdl.handle.net/21.15107/rcub_vinar_12945

Krstić D, Lalatović J, Mrđa D, Stojanović M, Savić N, Parac-Vogt T, Čolović M. Hafnium-containing Wells-Dawson nanocluster as a promising contrast agent candidate. in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings. 2024;2:39-42.
https://hdl.handle.net/21.15107/rcub_vinar_12945 .

Krstić, Danijela, Lalatović, Jovana, Mrđa, Davor, Stojanović, Marko, Savić, Nada, Parac-Vogt, Tatjana, Čolović, Mirjana, "Hafnium-containing Wells-Dawson nanocluster as a promising contrast agent candidate" in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings, 2 (2024):39-42,
https://hdl.handle.net/21.15107/rcub_vinar_12945 .

TY  - CONF
AU  - Lalatović, Jovana
AU  - Čolović, Mirjana
AU  - Mrđa, Davor
AU  - Stojanović, Marko
AU  - Krstić, Danijela
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12946
AB  - In this study contrast properties for computed tomography (CT) of two tungstate-containing molecules differing in size, shape, and structure: Keggin type polyoxometalate, 12-tungstosilicic acid, WSiA and sodium tungstate, Na2WO4, were assessed in vitro. In this aim, X-ray attenuation was monitored in vitro at different tungsten concentrations in the range of 3.125-100 mM. A stronger X-ray attenuation was observed for Na2WO4 in the presence of the same tungsten concentrations. In addition, both Na2WO4 and WSiA demonstrated better contrast performance compared to iodine-based iohexol solution, which has been widely applied as a contrast agent for CT. Accordingly, tungsten-containing compounds deserve attention as promising new-generation CT contrast candidates. Thus, future studies should be directed to the synthesis of novel tungsten molecules of different structures resulting in appropriate contrast and low toxicity.
C3  - International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings
T1  - Tungsten-based molecules of different structure as potential contrast agents
VL  - 2
SP  - 43
EP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12946
ER  - 

@conference{
author = "Lalatović, Jovana and Čolović, Mirjana and Mrđa, Davor and Stojanović, Marko and Krstić, Danijela",
year = "2024",
abstract = "In this study contrast properties for computed tomography (CT) of two tungstate-containing molecules differing in size, shape, and structure: Keggin type polyoxometalate, 12-tungstosilicic acid, WSiA and sodium tungstate, Na2WO4, were assessed in vitro. In this aim, X-ray attenuation was monitored in vitro at different tungsten concentrations in the range of 3.125-100 mM. A stronger X-ray attenuation was observed for Na2WO4 in the presence of the same tungsten concentrations. In addition, both Na2WO4 and WSiA demonstrated better contrast performance compared to iodine-based iohexol solution, which has been widely applied as a contrast agent for CT. Accordingly, tungsten-containing compounds deserve attention as promising new-generation CT contrast candidates. Thus, future studies should be directed to the synthesis of novel tungsten molecules of different structures resulting in appropriate contrast and low toxicity.",
journal = "International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings",
title = "Tungsten-based molecules of different structure as potential contrast agents",
volume = "2",
pages = "43-46",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12946"
}

Lalatović, J., Čolović, M., Mrđa, D., Stojanović, M.,& Krstić, D.. (2024). Tungsten-based molecules of different structure as potential contrast agents. in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings, 2, 43-46.
https://hdl.handle.net/21.15107/rcub_vinar_12946

Lalatović J, Čolović M, Mrđa D, Stojanović M, Krstić D. Tungsten-based molecules of different structure as potential contrast agents. in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings. 2024;2:43-46.
https://hdl.handle.net/21.15107/rcub_vinar_12946 .

Lalatović, Jovana, Čolović, Mirjana, Mrđa, Davor, Stojanović, Marko, Krstić, Danijela, "Tungsten-based molecules of different structure as potential contrast agents" in International Multidisciplinary Conference "Challenges of Contemporary Higher Education" - CCHE 2024 : Book of proceedings, 2 (2024):43-46,
https://hdl.handle.net/21.15107/rcub_vinar_12946 .

TY  - JOUR
AU  - Stojanović, Marko
AU  - Čolović, Mirjana B.
AU  - Lalatović, Jovana
AU  - Milosavljević, Aleksandra
AU  - Savić, Nada D.
AU  - Declerck, Kilian
AU  - Radosavljević, Branimir
AU  - Ćetković, Mila
AU  - Kravić-Stevović, Tamara
AU  - Parac-Vogt, Tatjana N.
AU  - Krstić, Danijela
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12867
AB  - Polyoxotungstate nanoclusters have recently emerged as promising contrast agents for computed tomography (CT). In order to evaluate their clinical potential, in this study, we evaluated the in vitro CT imaging properties, potential toxic effects in vivo, and tissue distribution of monolacunary Wells–Dawson polyoxometalate, α2-K10P2W17O61.20H2O (mono-WD POM). Mono-WD POM showed superior X-ray attenuation compared to other tungsten-containing nanoclusters (its parent WD-POM and Keggin POM) and the standard iodine-based contrast agent (iohexol). The calculated X-ray attenuation linear slope for mono-WD POM was significantly higher compared to parent WD-POM, Keggin POM, and iohexol (5.97 ± 0.14 vs. 4.84 ± 0.05, 4.55 ± 0.16, and 4.30 ± 0.09, respectively). Acute oral (maximum-administered dose (MAD) = 960 mg/kg) and intravenous administration (1/10, 1/5, and 1/3 MAD) of mono-WD POM did not induce unexpected changes in rats’ general habits or mortality. Results of blood gas analysis, CO-oximetry status, and the levels of electrolytes, glucose, lactate, creatinine, and BUN demonstrated a dose-dependent tendency 14 days after intravenous administration of mono-WD POM. The most significant differences compared to the control were observed for 1/3 MAD, being approximately seventy times higher than the typically used dose (0.015 mmol W/kg) of tungsten-based contrast agents. The highest tungsten deposition was found in the kidney (1/3 MAD—0.67 ± 0.12; 1/5 MAD—0.59 ± 0.07; 1/10 MAD—0.54 ± 0.05), which corresponded to detected morphological irregularities, electrolyte imbalance, and increased BUN levels.
T2  - International Journal of Molecular Sciences
T1  - Monolacunary Wells-Dawson Polyoxometalate as a Novel Contrast Agent for Computed Tomography: A Comprehensive Study on In Vivo Toxicity and Biodistribution
VL  - 25
IS  - 5
SP  - 2569
DO  - 10.3390/ijms25052569
ER  - 

@article{
author = "Stojanović, Marko and Čolović, Mirjana B. and Lalatović, Jovana and Milosavljević, Aleksandra and Savić, Nada D. and Declerck, Kilian and Radosavljević, Branimir and Ćetković, Mila and Kravić-Stevović, Tamara and Parac-Vogt, Tatjana N. and Krstić, Danijela",
year = "2024",
abstract = "Polyoxotungstate nanoclusters have recently emerged as promising contrast agents for computed tomography (CT). In order to evaluate their clinical potential, in this study, we evaluated the in vitro CT imaging properties, potential toxic effects in vivo, and tissue distribution of monolacunary Wells–Dawson polyoxometalate, α2-K10P2W17O61.20H2O (mono-WD POM). Mono-WD POM showed superior X-ray attenuation compared to other tungsten-containing nanoclusters (its parent WD-POM and Keggin POM) and the standard iodine-based contrast agent (iohexol). The calculated X-ray attenuation linear slope for mono-WD POM was significantly higher compared to parent WD-POM, Keggin POM, and iohexol (5.97 ± 0.14 vs. 4.84 ± 0.05, 4.55 ± 0.16, and 4.30 ± 0.09, respectively). Acute oral (maximum-administered dose (MAD) = 960 mg/kg) and intravenous administration (1/10, 1/5, and 1/3 MAD) of mono-WD POM did not induce unexpected changes in rats’ general habits or mortality. Results of blood gas analysis, CO-oximetry status, and the levels of electrolytes, glucose, lactate, creatinine, and BUN demonstrated a dose-dependent tendency 14 days after intravenous administration of mono-WD POM. The most significant differences compared to the control were observed for 1/3 MAD, being approximately seventy times higher than the typically used dose (0.015 mmol W/kg) of tungsten-based contrast agents. The highest tungsten deposition was found in the kidney (1/3 MAD—0.67 ± 0.12; 1/5 MAD—0.59 ± 0.07; 1/10 MAD—0.54 ± 0.05), which corresponded to detected morphological irregularities, electrolyte imbalance, and increased BUN levels.",
journal = "International Journal of Molecular Sciences",
title = "Monolacunary Wells-Dawson Polyoxometalate as a Novel Contrast Agent for Computed Tomography: A Comprehensive Study on In Vivo Toxicity and Biodistribution",
volume = "25",
number = "5",
pages = "2569",
doi = "10.3390/ijms25052569"
}

Stojanović, M., Čolović, M. B., Lalatović, J., Milosavljević, A., Savić, N. D., Declerck, K., Radosavljević, B., Ćetković, M., Kravić-Stevović, T., Parac-Vogt, T. N.,& Krstić, D.. (2024). Monolacunary Wells-Dawson Polyoxometalate as a Novel Contrast Agent for Computed Tomography: A Comprehensive Study on In Vivo Toxicity and Biodistribution. in International Journal of Molecular Sciences, 25(5), 2569.
https://doi.org/10.3390/ijms25052569

Stojanović M, Čolović MB, Lalatović J, Milosavljević A, Savić ND, Declerck K, Radosavljević B, Ćetković M, Kravić-Stevović T, Parac-Vogt TN, Krstić D. Monolacunary Wells-Dawson Polyoxometalate as a Novel Contrast Agent for Computed Tomography: A Comprehensive Study on In Vivo Toxicity and Biodistribution. in International Journal of Molecular Sciences. 2024;25(5):2569.
doi:10.3390/ijms25052569 .

Stojanović, Marko, Čolović, Mirjana B., Lalatović, Jovana, Milosavljević, Aleksandra, Savić, Nada D., Declerck, Kilian, Radosavljević, Branimir, Ćetković, Mila, Kravić-Stevović, Tamara, Parac-Vogt, Tatjana N., Krstić, Danijela, "Monolacunary Wells-Dawson Polyoxometalate as a Novel Contrast Agent for Computed Tomography: A Comprehensive Study on In Vivo Toxicity and Biodistribution" in International Journal of Molecular Sciences, 25, no. 5 (2024):2569,
https://doi.org/10.3390/ijms25052569 . .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Savić, Nada
AU  - Parac-Vogt, Tatjana
AU  - Krstić, Danijela Z.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12927
AB  - The aim of this study was to assess in vitro cytotoxic activity of a monolacunary Wells- Dawson nanocluster, α2-K10P2W17O61.20H2O (lacunary WD) against cervical carcinoma HeLa cells  as a commonly used model system for the evaluation of antitumor properties. After HeLa cells had been exposed to the investigated polyoxotungstate (the concentration range of 0.001 - 1 mM) for 24, 48, and 72 h, relative cell viability (expressed as a percentage of control) was determined.  The obtained results showed that lacunary WD affected HeLa cell viability in a concentration- and time-dependent manner. IC50 values (in μM), calculated using sigmoidal fitting experimental  plots, were as follows: 24.11 ± 9.95, 12.74 ± 0.096, and 11.48 ± 0.12 for 24, 48, and 72 hours treatment, respectively. In comparison with cisplatin, (positive control), IC50 values (μM) for 24 hours treatment were similar – 24.11 (lacunary WD) vs. 24.49 (cisplatin). However, after 48 and 72 hours IC50 obtained for cisplatin were found to be lower – 8.81 and 4.93 μM, respectively. Accordingly, the studied WD polyoxotungstate could not be regarded as a superior anticancer agent in comparison with the standard chemotherapeutic. Nevertheless, this studied nanocluster deserves attention as a promising antitumor therapeutic and as a good platform for the design of next-generation metal-based anticancer agents.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - In vitro cytotoxic activity of a monolacunary Wells-Dawson nanocluster against cervical carcinoma HeLa cells
SP  - 415
EP  - 418
DO  - 10.46793/ICCBI23.415C
ER  - 

@conference{
author = "Čolović, Mirjana and Žakula, Jelena and Korićanac, Lela and Savić, Nada and Parac-Vogt, Tatjana and Krstić, Danijela Z.",
year = "2023",
abstract = "The aim of this study was to assess in vitro cytotoxic activity of a monolacunary Wells- Dawson nanocluster, α2-K10P2W17O61.20H2O (lacunary WD) against cervical carcinoma HeLa cells  as a commonly used model system for the evaluation of antitumor properties. After HeLa cells had been exposed to the investigated polyoxotungstate (the concentration range of 0.001 - 1 mM) for 24, 48, and 72 h, relative cell viability (expressed as a percentage of control) was determined.  The obtained results showed that lacunary WD affected HeLa cell viability in a concentration- and time-dependent manner. IC50 values (in μM), calculated using sigmoidal fitting experimental  plots, were as follows: 24.11 ± 9.95, 12.74 ± 0.096, and 11.48 ± 0.12 for 24, 48, and 72 hours treatment, respectively. In comparison with cisplatin, (positive control), IC50 values (μM) for 24 hours treatment were similar – 24.11 (lacunary WD) vs. 24.49 (cisplatin). However, after 48 and 72 hours IC50 obtained for cisplatin were found to be lower – 8.81 and 4.93 μM, respectively. Accordingly, the studied WD polyoxotungstate could not be regarded as a superior anticancer agent in comparison with the standard chemotherapeutic. Nevertheless, this studied nanocluster deserves attention as a promising antitumor therapeutic and as a good platform for the design of next-generation metal-based anticancer agents.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "In vitro cytotoxic activity of a monolacunary Wells-Dawson nanocluster against cervical carcinoma HeLa cells",
pages = "415-418",
doi = "10.46793/ICCBI23.415C"
}

Čolović, M., Žakula, J., Korićanac, L., Savić, N., Parac-Vogt, T.,& Krstić, D. Z.. (2023). In vitro cytotoxic activity of a monolacunary Wells-Dawson nanocluster against cervical carcinoma HeLa cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 415-418.
https://doi.org/10.46793/ICCBI23.415C

Čolović M, Žakula J, Korićanac L, Savić N, Parac-Vogt T, Krstić DZ. In vitro cytotoxic activity of a monolacunary Wells-Dawson nanocluster against cervical carcinoma HeLa cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:415-418.
doi:10.46793/ICCBI23.415C .

Čolović, Mirjana, Žakula, Jelena, Korićanac, Lela, Savić, Nada, Parac-Vogt, Tatjana, Krstić, Danijela Z., "In vitro cytotoxic activity of a monolacunary Wells-Dawson nanocluster against cervical carcinoma HeLa cells" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):415-418,
https://doi.org/10.46793/ICCBI23.415C . .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Savić, Nada
AU  - Parac-Vogt, Tatjana
AU  - Krstić, Danijela Z.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12928
AB  - The objective of this study was to evaluate in vitro the antitumor properties of Fe(III)- substituted monolacunary Wells-Dawson polyoxotungstate, K7[FeIII(α2-P2W17O61)(H2O)] (FeWD) using cervical carcinoma HeLa cells as a model system. HeLa cells were exposed in vitro to FeWD within the concentration range from 0.001 to 1 mM, for 24, 48, and 72 hours. The studied Fe(III)- substituted polyoxotungstate affected HeLa cell viability in a concentration- and time-dependent manner. The obtained IC50 values (µM), as an indicator of the cytotoxic potential of FeWD, were: 16.64 ± 0.49, 10.75 ± 0.97, and 9.64 ± 0.19 for 24-, 48-, and 72-hour treatment, respectively. FeWD exhibited a stronger antitumor potential against HeLa cells than the structurally similar monolacunary Wells-Dawson polyoxotungstate, K10P2W17O61.20H2O (lacunary WD). Lacunary WD achieved IC50 at 24,11 µM after 24-hour exposure, which is about 44% higher concentration compared to the corresponding IC50 obtained for FeWD. This indicates that incorporating Fe(III) might be a new strategy for improving the antitumor efficacy of polyoxometalates as promising candidates for next-generation chemotherapeutics.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - The influence of Fe(III) incorporation on anti-cancer potential of a Wells-Dawson nanocluster
SP  - 419
EP  - 422
DO  - 10.46793/ICCBI23.419C
ER  - 

@conference{
author = "Čolović, Mirjana and Korićanac, Lela and Žakula, Jelena and Savić, Nada and Parac-Vogt, Tatjana and Krstić, Danijela Z.",
year = "2023",
abstract = "The objective of this study was to evaluate in vitro the antitumor properties of Fe(III)- substituted monolacunary Wells-Dawson polyoxotungstate, K7[FeIII(α2-P2W17O61)(H2O)] (FeWD) using cervical carcinoma HeLa cells as a model system. HeLa cells were exposed in vitro to FeWD within the concentration range from 0.001 to 1 mM, for 24, 48, and 72 hours. The studied Fe(III)- substituted polyoxotungstate affected HeLa cell viability in a concentration- and time-dependent manner. The obtained IC50 values (µM), as an indicator of the cytotoxic potential of FeWD, were: 16.64 ± 0.49, 10.75 ± 0.97, and 9.64 ± 0.19 for 24-, 48-, and 72-hour treatment, respectively. FeWD exhibited a stronger antitumor potential against HeLa cells than the structurally similar monolacunary Wells-Dawson polyoxotungstate, K10P2W17O61.20H2O (lacunary WD). Lacunary WD achieved IC50 at 24,11 µM after 24-hour exposure, which is about 44% higher concentration compared to the corresponding IC50 obtained for FeWD. This indicates that incorporating Fe(III) might be a new strategy for improving the antitumor efficacy of polyoxometalates as promising candidates for next-generation chemotherapeutics.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "The influence of Fe(III) incorporation on anti-cancer potential of a Wells-Dawson nanocluster",
pages = "419-422",
doi = "10.46793/ICCBI23.419C"
}

Čolović, M., Korićanac, L., Žakula, J., Savić, N., Parac-Vogt, T.,& Krstić, D. Z.. (2023). The influence of Fe(III) incorporation on anti-cancer potential of a Wells-Dawson nanocluster. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 419-422.
https://doi.org/10.46793/ICCBI23.419C

Čolović M, Korićanac L, Žakula J, Savić N, Parac-Vogt T, Krstić DZ. The influence of Fe(III) incorporation on anti-cancer potential of a Wells-Dawson nanocluster. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:419-422.
doi:10.46793/ICCBI23.419C .

Čolović, Mirjana, Korićanac, Lela, Žakula, Jelena, Savić, Nada, Parac-Vogt, Tatjana, Krstić, Danijela Z., "The influence of Fe(III) incorporation on anti-cancer potential of a Wells-Dawson nanocluster" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):419-422,
https://doi.org/10.46793/ICCBI23.419C . .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Gajski, Goran
AU  - Gerić, M.
AU  - Domijan, Ana-Marija
AU  - Savić, N.
AU  - Parac-Vogt, Tatjana
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12929
AB  - UVOD: Istraživanja pokazuju da su metalne nanočestice pogodni kandidati za kontrastna sredstva
(CESA-contrast-enhancing staining agents) nove generacije za kompjuterizovanu tomografiju (CT)
zahvaljujući velikoj gustini i sposobnosti da apsorbuju X-zrake.
CILJ: Studije toksičnosti in vivo ukazuju na štetno dejstvo polioksometalata, što bi mogla biti glavna
prepreka u kliničkoj primeni ovih bioaktivnih kompleksnih jedinjenja. Stoga je cilj ovog istraživanja da
se ispitaju genotoksične osobine polioksovolframata Wells-Dawson tipa supstituisanih sa hafnijumom
(Hf-WD 1:2), K16[Hf(α2-P2W17O61)2]·19H2O, koji je pokazao dobre osobine kao kontrastno sredstvo za
vizuelizaciju tkiva bubrega i dugih kostiju miša.
METODOLOGIJA: Hf-WD 1:2 je sintetisan prema metodi opisanoj u literaturi. Uzorci humane pune krvi
su uzeti od zdravih donora i izloženi različitim koncentracijama (10-6-10-4 mol/L) Hf-WD 1:2 tokom 4
i 24 sata, na 37 °C. Standardna metoda za alkalni komet test je korišćena za praćenje genotoksičnih
efekata Hf-WD 1:2.
REZULTATI: Stepen oštećenja DNK humane periferne krvi nakon izlaganja Hf-WD 1:2 je izražen kao relativno
povećanje repne DNK u odnosu na kontrolu. Nije uočena statistički značajna razlika u količini prekida
DNK lanca u poređenju sa odgovarajućom kontrolom, za oba vremena izlaganja (4 i 24 sata) i za sve
ispitivane koncentracije polioksovolframata.
ZAKLJUČAK: In vitro ispitivanje genotoksičnosti pokazalo je da Hf-WD 1:2 ne menja značajno strukturu
DNK zdravih humanih ćelija pune krvi i stoga se može smatrati bezbednim u smislu genotoksičnosti u
dodatnim studijama kao potencijalnog kontrastnog sredstva za CT.
AB  - INTRODUCTION: Metallic nanoparticles have been reported as promising candidates for the development
of new-generation contrast-enhancing staining agents (CESAs) for computed tomography (CT)
due to their high X-ray attenuation and density.
AIM: In vivo toxicity studies revealed side effects of polyoxometalate clusters, which could be a key
obstacle in the potential clinical application of these bioactive compounds. Therefore, the aim of this
investigation was to perform the genotoxicity evaluation of 1:2 hafnium(IV)-substituted Wells-Dawson
polyoxotungstate (Hf-WD 1:2), K16[Hf(α2-P2W17O61)2]·19H2O that was reported as a promising CESA
candidate to visualize murine long bones and kidneys.
METHODOLOGY: Hf-WD 1:2 was prepared using the method described in the literature. Human whole
blood samples were taken from healthy donors and exposed to different Hf-WD 1:2 concentrations
(within the range of 10-6-10-4 mol/L) for 4 and 24 h, at 37 °C. The standard procedure for alkaline comet
assay was carried out for monitoring the genotoxic effects of Hf-WD 1:2.
RESULTS: The degree of DNA damage in human peripheral blood cells after exposure to Hf-WD 1:2 was
expressed as a relative increase of tail DNA related to the control. No statistically significant difference
in the amount of DNA strand breaks in comparison with the corresponding control was observed for
both 4 and 24 h, regardless of the Hf-WD 1:2 concentrations tested.
CONCLUSION: In vitro genotoxicity assessment indicated that Hf-WD 1:2 does not affect DNA structure
in healthy human blood cells, accordingly might be regarded as genotoxicity safe in further research as
a potential CESA for CT.
PB  - Belgrade : Serbian Society of Toxicology
C3  - 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book
T1  - Wells-Dawson polioksovolframati supstituisani hafnijumom kao potencijalna kontrastna sredstva: uticaj na DNK in vitro
T1  - Hafnium(IV)-substituted Wells-Dawson based contrast-enhancing staining agent: effect on DNA in vitro
SP  - 189
EP  - 190
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12929
ER  - 

@conference{
author = "Čolović, Mirjana and Gajski, Goran and Gerić, M. and Domijan, Ana-Marija and Savić, N. and Parac-Vogt, Tatjana and Krstić, Danijela",
year = "2023",
abstract = "UVOD: Istraživanja pokazuju da su metalne nanočestice pogodni kandidati za kontrastna sredstva
(CESA-contrast-enhancing staining agents) nove generacije za kompjuterizovanu tomografiju (CT)
zahvaljujući velikoj gustini i sposobnosti da apsorbuju X-zrake.
CILJ: Studije toksičnosti in vivo ukazuju na štetno dejstvo polioksometalata, što bi mogla biti glavna
prepreka u kliničkoj primeni ovih bioaktivnih kompleksnih jedinjenja. Stoga je cilj ovog istraživanja da
se ispitaju genotoksične osobine polioksovolframata Wells-Dawson tipa supstituisanih sa hafnijumom
(Hf-WD 1:2), K16[Hf(α2-P2W17O61)2]·19H2O, koji je pokazao dobre osobine kao kontrastno sredstvo za
vizuelizaciju tkiva bubrega i dugih kostiju miša.
METODOLOGIJA: Hf-WD 1:2 je sintetisan prema metodi opisanoj u literaturi. Uzorci humane pune krvi
su uzeti od zdravih donora i izloženi različitim koncentracijama (10-6-10-4 mol/L) Hf-WD 1:2 tokom 4
i 24 sata, na 37 °C. Standardna metoda za alkalni komet test je korišćena za praćenje genotoksičnih
efekata Hf-WD 1:2.
REZULTATI: Stepen oštećenja DNK humane periferne krvi nakon izlaganja Hf-WD 1:2 je izražen kao relativno
povećanje repne DNK u odnosu na kontrolu. Nije uočena statistički značajna razlika u količini prekida
DNK lanca u poređenju sa odgovarajućom kontrolom, za oba vremena izlaganja (4 i 24 sata) i za sve
ispitivane koncentracije polioksovolframata.
ZAKLJUČAK: In vitro ispitivanje genotoksičnosti pokazalo je da Hf-WD 1:2 ne menja značajno strukturu
DNK zdravih humanih ćelija pune krvi i stoga se može smatrati bezbednim u smislu genotoksičnosti u
dodatnim studijama kao potencijalnog kontrastnog sredstva za CT., INTRODUCTION: Metallic nanoparticles have been reported as promising candidates for the development
of new-generation contrast-enhancing staining agents (CESAs) for computed tomography (CT)
due to their high X-ray attenuation and density.
AIM: In vivo toxicity studies revealed side effects of polyoxometalate clusters, which could be a key
obstacle in the potential clinical application of these bioactive compounds. Therefore, the aim of this
investigation was to perform the genotoxicity evaluation of 1:2 hafnium(IV)-substituted Wells-Dawson
polyoxotungstate (Hf-WD 1:2), K16[Hf(α2-P2W17O61)2]·19H2O that was reported as a promising CESA
candidate to visualize murine long bones and kidneys.
METHODOLOGY: Hf-WD 1:2 was prepared using the method described in the literature. Human whole
blood samples were taken from healthy donors and exposed to different Hf-WD 1:2 concentrations
(within the range of 10-6-10-4 mol/L) for 4 and 24 h, at 37 °C. The standard procedure for alkaline comet
assay was carried out for monitoring the genotoxic effects of Hf-WD 1:2.
RESULTS: The degree of DNA damage in human peripheral blood cells after exposure to Hf-WD 1:2 was
expressed as a relative increase of tail DNA related to the control. No statistically significant difference
in the amount of DNA strand breaks in comparison with the corresponding control was observed for
both 4 and 24 h, regardless of the Hf-WD 1:2 concentrations tested.
CONCLUSION: In vitro genotoxicity assessment indicated that Hf-WD 1:2 does not affect DNA structure
in healthy human blood cells, accordingly might be regarded as genotoxicity safe in further research as
a potential CESA for CT.",
publisher = "Belgrade : Serbian Society of Toxicology",
journal = "13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book",
title = "Wells-Dawson polioksovolframati supstituisani hafnijumom kao potencijalna kontrastna sredstva: uticaj na DNK in vitro, Hafnium(IV)-substituted Wells-Dawson based contrast-enhancing staining agent: effect on DNA in vitro",
pages = "189-190",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12929"
}

Čolović, M., Gajski, G., Gerić, M., Domijan, A., Savić, N., Parac-Vogt, T.,& Krstić, D.. (2023). Wells-Dawson polioksovolframati supstituisani hafnijumom kao potencijalna kontrastna sredstva: uticaj na DNK in vitro. in 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book
Belgrade : Serbian Society of Toxicology., 189-190.
https://hdl.handle.net/21.15107/rcub_vinar_12929

Čolović M, Gajski G, Gerić M, Domijan A, Savić N, Parac-Vogt T, Krstić D. Wells-Dawson polioksovolframati supstituisani hafnijumom kao potencijalna kontrastna sredstva: uticaj na DNK in vitro. in 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book. 2023;:189-190.
https://hdl.handle.net/21.15107/rcub_vinar_12929 .

Čolović, Mirjana, Gajski, Goran, Gerić, M., Domijan, Ana-Marija, Savić, N., Parac-Vogt, Tatjana, Krstić, Danijela, "Wells-Dawson polioksovolframati supstituisani hafnijumom kao potencijalna kontrastna sredstva: uticaj na DNK in vitro" in 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book (2023):189-190,
https://hdl.handle.net/21.15107/rcub_vinar_12929 .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Gajski, Goran
AU  - Gerić, M.
AU  - Domijan, Ana-Marija
AU  - Savić, N.
AU  - Parac-Vogt, Tatjana
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12930
AB  - UVOD: Polioksovolframati su negativno naelektrisana neorganska kompleksna jedinjenja koja se dve
decenije intenzivno proučavaju kao moćni bioaktivni agensi. Skorašnje studije na dugim kostima i
bubrezima miša su pokazale da poliokosvoframati mogu da se koriste kao agensi za bojenje za poboljšanje
kontrasta (CESAs) za kompjuterizovanu tomografiju (CT).
CILJ: S obzirom da razvoj novih medicinskih agenasa zahteva procenu njihove bezbednosti, cilj ove
studije je da se evaluiraju genotoksične osobine in vitro polioksovolframata Wells-Dawson tipa (parent
WD), α2-K6P2W18O62.14H2O, koji je u prethodnim istraživanjima pokazao bolje osobine u odnosu na
joheksol, standardni kontrastni agens za CT.
METODOLOGIJA: Parent WD je sintetisan sledeći ovjavljenu proceduru. Uzorci humane krvi dobijeni od
zdravih donora su tretirani ispitivanim polioksovolframatom, a zatim inkubirani na 37 °C tokom 4 i 24
sata. Za procenu genotoksičnosti parent WD korišćen je alkalni komet test kako je opisano u literaturi.
REZULTATI: Uzorci pune krvi su tretirani parent WD polioksovolframatom u opsegu koncentracija
1-100 μmol/L. Oštećenje DNK, koje se koristi kao pokazatelj genotoksičnosti, je izraženo kao % repne
DNK. Dobijeni rezultati su pokazali da sve ispitivane koncentracije parent WD nisu značajno uticale na
oštećenje DNK u odnosu na odgovarajuću kontrolu, posle 4 i 24 sata tretmana.
ZAKLJUČAK: Parent WD nanoklaster nije izazvao genotoksični efekat na zdravim ćelijama periferne
humane krvi u svim ispitivanim koncentracijama. U skladu sa tim, ispitivani potencijalni CESA kandidat
za CT bi se u daljim istraživanjima mogao smatrati bezbednim sa stanovišta genotoksičnosti.
AB  - INTRODUCTION: Polyoxotungstates are negatively charged inorganic cage complexes that were studied
as potent bioactive agents for the last two decades. They were recently reported as promising
contrast-enhancing staining agents (CESAs) for computed tomography (CT) of murine long bones and
kidney tissues.
AIM: Taking into account the fact that the development of novel medications requires an evaluation
of their safety, the purpose of this study was to assess the genotoxic properties in vitro of parent
Wells-Dawson polyoxotungstate (parent WD), α2-K6P2W18O62.14H2O that showed superior imaging
capabilities compared to the standard CT contrast agent iohexol.
METHODOLOGY: Parent WD was synthesized according to the published procedure. Human whole
blood samples obtained from healthy donors were treated with parent WD, and then incubated at 37
°C for 4 and 24 h. For the genotoxicity assessment of the studied nanocluster alkaline comet assay was
performed as described in the literature.
RESULTS: Whole blood samples were treated with parent WD within the concentration range of 1-100
μmol/L. DNA damage was expressed as % of tail DNA and used as the indicator of genotoxicity. The
obtained results demonstrated that parent WD at all investigated concentrations did not significantly
affect DNA damage with respect to the corresponding control, for both 4 and 24 h.
CONCLUSION: Parent WD nanocluster did not induce a genotoxic effect on normal non-target human
peripheral blood cells at all studied concentrations. Thus, this promising CESA candidate for CT could
be considered in further research as safe from a genotoxicity point of view.
PB  - Belgrade : Serbian Society of Toxicology
C3  - 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book
T1  - Evaluacija genotoksičnosti nanoklastera polioksovolframata kao potencijalnog kontrastnog agensa
T1  - Genotoxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent
SP  - 191
EP  - 192
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12930
ER  - 

@conference{
author = "Čolović, Mirjana and Gajski, Goran and Gerić, M. and Domijan, Ana-Marija and Savić, N. and Parac-Vogt, Tatjana and Krstić, Danijela",
year = "2023",
abstract = "UVOD: Polioksovolframati su negativno naelektrisana neorganska kompleksna jedinjenja koja se dve
decenije intenzivno proučavaju kao moćni bioaktivni agensi. Skorašnje studije na dugim kostima i
bubrezima miša su pokazale da poliokosvoframati mogu da se koriste kao agensi za bojenje za poboljšanje
kontrasta (CESAs) za kompjuterizovanu tomografiju (CT).
CILJ: S obzirom da razvoj novih medicinskih agenasa zahteva procenu njihove bezbednosti, cilj ove
studije je da se evaluiraju genotoksične osobine in vitro polioksovolframata Wells-Dawson tipa (parent
WD), α2-K6P2W18O62.14H2O, koji je u prethodnim istraživanjima pokazao bolje osobine u odnosu na
joheksol, standardni kontrastni agens za CT.
METODOLOGIJA: Parent WD je sintetisan sledeći ovjavljenu proceduru. Uzorci humane krvi dobijeni od
zdravih donora su tretirani ispitivanim polioksovolframatom, a zatim inkubirani na 37 °C tokom 4 i 24
sata. Za procenu genotoksičnosti parent WD korišćen je alkalni komet test kako je opisano u literaturi.
REZULTATI: Uzorci pune krvi su tretirani parent WD polioksovolframatom u opsegu koncentracija
1-100 μmol/L. Oštećenje DNK, koje se koristi kao pokazatelj genotoksičnosti, je izraženo kao % repne
DNK. Dobijeni rezultati su pokazali da sve ispitivane koncentracije parent WD nisu značajno uticale na
oštećenje DNK u odnosu na odgovarajuću kontrolu, posle 4 i 24 sata tretmana.
ZAKLJUČAK: Parent WD nanoklaster nije izazvao genotoksični efekat na zdravim ćelijama periferne
humane krvi u svim ispitivanim koncentracijama. U skladu sa tim, ispitivani potencijalni CESA kandidat
za CT bi se u daljim istraživanjima mogao smatrati bezbednim sa stanovišta genotoksičnosti., INTRODUCTION: Polyoxotungstates are negatively charged inorganic cage complexes that were studied
as potent bioactive agents for the last two decades. They were recently reported as promising
contrast-enhancing staining agents (CESAs) for computed tomography (CT) of murine long bones and
kidney tissues.
AIM: Taking into account the fact that the development of novel medications requires an evaluation
of their safety, the purpose of this study was to assess the genotoxic properties in vitro of parent
Wells-Dawson polyoxotungstate (parent WD), α2-K6P2W18O62.14H2O that showed superior imaging
capabilities compared to the standard CT contrast agent iohexol.
METHODOLOGY: Parent WD was synthesized according to the published procedure. Human whole
blood samples obtained from healthy donors were treated with parent WD, and then incubated at 37
°C for 4 and 24 h. For the genotoxicity assessment of the studied nanocluster alkaline comet assay was
performed as described in the literature.
RESULTS: Whole blood samples were treated with parent WD within the concentration range of 1-100
μmol/L. DNA damage was expressed as % of tail DNA and used as the indicator of genotoxicity. The
obtained results demonstrated that parent WD at all investigated concentrations did not significantly
affect DNA damage with respect to the corresponding control, for both 4 and 24 h.
CONCLUSION: Parent WD nanocluster did not induce a genotoxic effect on normal non-target human
peripheral blood cells at all studied concentrations. Thus, this promising CESA candidate for CT could
be considered in further research as safe from a genotoxicity point of view.",
publisher = "Belgrade : Serbian Society of Toxicology",
journal = "13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book",
title = "Evaluacija genotoksičnosti nanoklastera polioksovolframata kao potencijalnog kontrastnog agensa, Genotoxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent",
pages = "191-192",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12930"
}

Čolović, M., Gajski, G., Gerić, M., Domijan, A., Savić, N., Parac-Vogt, T.,& Krstić, D.. (2023). Evaluacija genotoksičnosti nanoklastera polioksovolframata kao potencijalnog kontrastnog agensa. in 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book
Belgrade : Serbian Society of Toxicology., 191-192.
https://hdl.handle.net/21.15107/rcub_vinar_12930

Čolović M, Gajski G, Gerić M, Domijan A, Savić N, Parac-Vogt T, Krstić D. Evaluacija genotoksičnosti nanoklastera polioksovolframata kao potencijalnog kontrastnog agensa. in 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book. 2023;:191-192.
https://hdl.handle.net/21.15107/rcub_vinar_12930 .

Čolović, Mirjana, Gajski, Goran, Gerić, M., Domijan, Ana-Marija, Savić, N., Parac-Vogt, Tatjana, Krstić, Danijela, "Evaluacija genotoksičnosti nanoklastera polioksovolframata kao potencijalnog kontrastnog agensa" in 13th International Congress of the Serbian Society of Toxicology and the 1st ToxSEE Regional Conference : Abstract book (2023):191-192,
https://hdl.handle.net/21.15107/rcub_vinar_12930 .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Savić, N.
AU  - Parac-Vogt, Tatjana
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12931
AB  - Polyoxometalates are negatively charged polyanions containing early transition metal ions in their high oxidation state surrounded by bridged oxygen. Firstly, these metal-based clusters were used as promising agents in electron-dense imaging, separations, catalysis, and analysis. In recent years, numerous studies in vitro and in vivo found that these nanocomplexes possess a variety of biological effects including antidiabetic, anticancer, and antibiotic actions. Despite these observed properties, the mechanism of their biological activities has not been completely elucidated so far. On the other hand, the results of enzymatic studies revealed their inhibiting influence on physiologically important extracellular enzymes such as phosphatases, esterases, and ecto-nucleotidases, which are considered target enzymes for the approved biological actions. Accordingly, the overview of the in vitro influence of selected polyoxo-vanadates, -tungstates, and – palladates on cholinesterase, ATPase, and phosphatase activities will be given in this presentation. Cholinesterases, enzymes located on the postsynaptic plasma membrane, have a key role in nerve impulse transmission and were confirmed as the targets of drugs for neurological diseases, which are regularly used in clinical practice. Moreover, ATPases and phosphatases were found to be included in the proliferation and migration of tumor cells, thus the inhibition of these enzymes was found as the mechanism of some anticancer drug action
C3  - Medical Research : Medicinska istraživanja
T1  - Enzymes as a platform for drug development
VL  - 56
IS  - 4
SP  - 130
EP  - 130
DO  - 10.5937/medi56-47579
ER  - 

@conference{
author = "Čolović, Mirjana and Savić, N. and Parac-Vogt, Tatjana and Krstić, Danijela",
year = "2023",
abstract = "Polyoxometalates are negatively charged polyanions containing early transition metal ions in their high oxidation state surrounded by bridged oxygen. Firstly, these metal-based clusters were used as promising agents in electron-dense imaging, separations, catalysis, and analysis. In recent years, numerous studies in vitro and in vivo found that these nanocomplexes possess a variety of biological effects including antidiabetic, anticancer, and antibiotic actions. Despite these observed properties, the mechanism of their biological activities has not been completely elucidated so far. On the other hand, the results of enzymatic studies revealed their inhibiting influence on physiologically important extracellular enzymes such as phosphatases, esterases, and ecto-nucleotidases, which are considered target enzymes for the approved biological actions. Accordingly, the overview of the in vitro influence of selected polyoxo-vanadates, -tungstates, and – palladates on cholinesterase, ATPase, and phosphatase activities will be given in this presentation. Cholinesterases, enzymes located on the postsynaptic plasma membrane, have a key role in nerve impulse transmission and were confirmed as the targets of drugs for neurological diseases, which are regularly used in clinical practice. Moreover, ATPases and phosphatases were found to be included in the proliferation and migration of tumor cells, thus the inhibition of these enzymes was found as the mechanism of some anticancer drug action",
journal = "Medical Research : Medicinska istraživanja",
title = "Enzymes as a platform for drug development",
volume = "56",
number = "4",
pages = "130-130",
doi = "10.5937/medi56-47579"
}

Čolović, M., Savić, N., Parac-Vogt, T.,& Krstić, D.. (2023). Enzymes as a platform for drug development. in Medical Research : Medicinska istraživanja, 56(4), 130-130.
https://doi.org/10.5937/medi56-47579

Čolović M, Savić N, Parac-Vogt T, Krstić D. Enzymes as a platform for drug development. in Medical Research : Medicinska istraživanja. 2023;56(4):130-130.
doi:10.5937/medi56-47579 .

Čolović, Mirjana, Savić, N., Parac-Vogt, Tatjana, Krstić, Danijela, "Enzymes as a platform for drug development" in Medical Research : Medicinska istraživanja, 56, no. 4 (2023):130-130,
https://doi.org/10.5937/medi56-47579 . .

TY  - CONF
AU  - Parac-Vogt, Tatjana
AU  - Savić, Nada
AU  - Kerckhofs, Greet
AU  - Stojanović, Marko
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12940
AB  - Due to the high complexity and heterogeneous structure of biological tissues, imaging techniques that allow for precise and quantitative structural analyses of such materials are of high importance. These techniques could also advance clinical translation of regenerative medicine by providing better insights in tissue development and disease. The standard techniques for evaluating biological tissues such as histological sectioning and staining, have a high discriminative power, but allow assessment of the tissue distribution only in two dimensions. Due to restricted sectioning orientation and limited depth resolution, this leads to loss of information in three dimensions (3D), and therefore, more precise imaging of the 3D microstructure and spatial interrelationships of the different tissues within organs is crucial. MicroCT can provide full 3D structural information of mineralized tissues and dense biomaterials. However, the intrinsic low X-ray absorption of soft tissues requires contrast-enhancing staining agents (CESAs) to be used. We have shown that a range of polyoxometalate clusters (POMs) can be excellent non-destructive staining agents for high-resolution contrast-enhanced microCT (CE-CT) visualization of various tissues, of bone and its marrow vascularization and adiposity. A range of Wells-Dawson POMs, differing in structure and overall charge, has been synthesized and evaluated for their potential as soft tissue CESAs. We have shown that hafnium-substituted POM (Hf-POM) allows for simultaneous contrast-enhanced microCT (CE-CT) visualization of bone and its marrow vascularization and adiposity. Monolacunary Wells-Dawson POM (Mono-WD POM) showed similar soft tissue enhancement as Hf-WD POM and phosphotungstic acid (PTA), a frequently used but destructive CESA. However, compared to PTA, the POMs are much less destructive and show a better diffusion. The solubility of Mono-WD POM can be improved by simple addition of lithium chloride to the staining solution, leading to further enhancement of the soft tissue contrast. In vivo toxicity of Wells-Dawson POM has been also evaluated according to standard toxicological protocols using Wistar albino rats, which is the first and important step in evaluating side effects of these polyoxometalate nanoclusters that show large potential as therapeutics and contrast agents.
C3  - Medical Research : Medicinska istraživanja
T1  - Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography
VL  - 56
IS  - 4
SP  - 129
EP  - 129
DO  - 10.5937/medi56-47579
ER  - 

@conference{
author = "Parac-Vogt, Tatjana and Savić, Nada and Kerckhofs, Greet and Stojanović, Marko and Čolović, Mirjana and Krstić, Danijela",
year = "2023",
abstract = "Due to the high complexity and heterogeneous structure of biological tissues, imaging techniques that allow for precise and quantitative structural analyses of such materials are of high importance. These techniques could also advance clinical translation of regenerative medicine by providing better insights in tissue development and disease. The standard techniques for evaluating biological tissues such as histological sectioning and staining, have a high discriminative power, but allow assessment of the tissue distribution only in two dimensions. Due to restricted sectioning orientation and limited depth resolution, this leads to loss of information in three dimensions (3D), and therefore, more precise imaging of the 3D microstructure and spatial interrelationships of the different tissues within organs is crucial. MicroCT can provide full 3D structural information of mineralized tissues and dense biomaterials. However, the intrinsic low X-ray absorption of soft tissues requires contrast-enhancing staining agents (CESAs) to be used. We have shown that a range of polyoxometalate clusters (POMs) can be excellent non-destructive staining agents for high-resolution contrast-enhanced microCT (CE-CT) visualization of various tissues, of bone and its marrow vascularization and adiposity. A range of Wells-Dawson POMs, differing in structure and overall charge, has been synthesized and evaluated for their potential as soft tissue CESAs. We have shown that hafnium-substituted POM (Hf-POM) allows for simultaneous contrast-enhanced microCT (CE-CT) visualization of bone and its marrow vascularization and adiposity. Monolacunary Wells-Dawson POM (Mono-WD POM) showed similar soft tissue enhancement as Hf-WD POM and phosphotungstic acid (PTA), a frequently used but destructive CESA. However, compared to PTA, the POMs are much less destructive and show a better diffusion. The solubility of Mono-WD POM can be improved by simple addition of lithium chloride to the staining solution, leading to further enhancement of the soft tissue contrast. In vivo toxicity of Wells-Dawson POM has been also evaluated according to standard toxicological protocols using Wistar albino rats, which is the first and important step in evaluating side effects of these polyoxometalate nanoclusters that show large potential as therapeutics and contrast agents.",
journal = "Medical Research : Medicinska istraživanja",
title = "Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography",
volume = "56",
number = "4",
pages = "129-129",
doi = "10.5937/medi56-47579"
}

Parac-Vogt, T., Savić, N., Kerckhofs, G., Stojanović, M., Čolović, M.,& Krstić, D.. (2023). Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography. in Medical Research : Medicinska istraživanja, 56(4), 129-129.
https://doi.org/10.5937/medi56-47579

Parac-Vogt T, Savić N, Kerckhofs G, Stojanović M, Čolović M, Krstić D. Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography. in Medical Research : Medicinska istraživanja. 2023;56(4):129-129.
doi:10.5937/medi56-47579 .

Parac-Vogt, Tatjana, Savić, Nada, Kerckhofs, Greet, Stojanović, Marko, Čolović, Mirjana, Krstić, Danijela, "Exploring polyoxometalates as non-destructive staining agents for contrast-enhanced computed tomography" in Medical Research : Medicinska istraživanja, 56, no. 4 (2023):129-129,
https://doi.org/10.5937/medi56-47579 . .

TY  - CONF
AU  - Stojanović, Marko
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12941
AB  - Toksikološka ispitivanja predstavljaju osnovu prekliničkih ispitivanja novih lekova i medicinskih sredstava. Ovaj tip studija može predstavljati presudni korak u procesu procene daljeg razvoja lekova i medicinskih sredstava. Svedoci smo sve većeg interesovanja industrije i šire akademske zajednice za ovaj tip istraživanja, pa je upoznavanje sa vodičima za sprovođenje ovih studija, potpomognuto primerima iz prakse od ključnog značaja za njihovu edukaciju. Uzimajući sve ovo u obzir neophodno je da šira akademska zajednica bude bolje upoznata sa značajem ovih studija i pravilnim načinom njihovog sprovođenja. Od velikog je značaja upoznati zainteresovane strane o načinu sprovođenja toksikoloških studija, pre svega sa zakonskim okvirima koji se moraju poštovati u toku njihovog sprovođenja. Neophodno je zainteresovanim stranama iz šire akademske zajednice i industrije ukazati na etičke izazove na koje se nailazi tokom sprovođenja jedne toksikološke studije, pogotovu ako se u obzir uzme koliko su ovakva ispitivanja sa etičkog aspekta osetljiva. Postoji i potreba da se razjasni kako ispitivanje različitih toksikoloških aspekata zahteva i različite pristupe u sprovođenju ovih studija. Tako se npr. međusobno se razlikuju studije koje ispituju akutnu ili hroničnu toksičnost, studije u kojima se ispituju različiti načini primene (per os, intravenski,...) lekova i medicinskih sredstva i sl. Sve ove informacije bi trebalo da razjasne zainteresovanim stranama kako pravilno sprovesti toksikološke studije i kako iz njih dobiti relevantne rezultate, a da se pri tome ne krše zakonske odredbe niti etičke norme.
C3  - Medical Research : Medicinska istraživanja
T1  - Toksikološka ispitivanja tokom razvijanja novih lekova i medicinskih sredstava
VL  - 56
IS  - 4
SP  - 131
EP  - 132
DO  - 10.5937/medi56-47579
ER  - 

@conference{
author = "Stojanović, Marko and Čolović, Mirjana and Krstić, Danijela",
year = "2023",
abstract = "Toksikološka ispitivanja predstavljaju osnovu prekliničkih ispitivanja novih lekova i medicinskih sredstava. Ovaj tip studija može predstavljati presudni korak u procesu procene daljeg razvoja lekova i medicinskih sredstava. Svedoci smo sve većeg interesovanja industrije i šire akademske zajednice za ovaj tip istraživanja, pa je upoznavanje sa vodičima za sprovođenje ovih studija, potpomognuto primerima iz prakse od ključnog značaja za njihovu edukaciju. Uzimajući sve ovo u obzir neophodno je da šira akademska zajednica bude bolje upoznata sa značajem ovih studija i pravilnim načinom njihovog sprovođenja. Od velikog je značaja upoznati zainteresovane strane o načinu sprovođenja toksikoloških studija, pre svega sa zakonskim okvirima koji se moraju poštovati u toku njihovog sprovođenja. Neophodno je zainteresovanim stranama iz šire akademske zajednice i industrije ukazati na etičke izazove na koje se nailazi tokom sprovođenja jedne toksikološke studije, pogotovu ako se u obzir uzme koliko su ovakva ispitivanja sa etičkog aspekta osetljiva. Postoji i potreba da se razjasni kako ispitivanje različitih toksikoloških aspekata zahteva i različite pristupe u sprovođenju ovih studija. Tako se npr. međusobno se razlikuju studije koje ispituju akutnu ili hroničnu toksičnost, studije u kojima se ispituju različiti načini primene (per os, intravenski,...) lekova i medicinskih sredstva i sl. Sve ove informacije bi trebalo da razjasne zainteresovanim stranama kako pravilno sprovesti toksikološke studije i kako iz njih dobiti relevantne rezultate, a da se pri tome ne krše zakonske odredbe niti etičke norme.",
journal = "Medical Research : Medicinska istraživanja",
title = "Toksikološka ispitivanja tokom razvijanja novih lekova i medicinskih sredstava",
volume = "56",
number = "4",
pages = "131-132",
doi = "10.5937/medi56-47579"
}

Stojanović, M., Čolović, M.,& Krstić, D.. (2023). Toksikološka ispitivanja tokom razvijanja novih lekova i medicinskih sredstava. in Medical Research : Medicinska istraživanja, 56(4), 131-132.
https://doi.org/10.5937/medi56-47579

Stojanović M, Čolović M, Krstić D. Toksikološka ispitivanja tokom razvijanja novih lekova i medicinskih sredstava. in Medical Research : Medicinska istraživanja. 2023;56(4):131-132.
doi:10.5937/medi56-47579 .

Stojanović, Marko, Čolović, Mirjana, Krstić, Danijela, "Toksikološka ispitivanja tokom razvijanja novih lekova i medicinskih sredstava" in Medical Research : Medicinska istraživanja, 56, no. 4 (2023):131-132,
https://doi.org/10.5937/medi56-47579 . .

TY  - CONF
AU  - Dinčić, Marko
AU  - Todorović, Jasna
AU  - Čolović, Mirjana
AU  - Ćetković Milisavljević, Mila
AU  - Kravić Stevović, Tamara
AU  - Milosavljević, Aleksandra
AU  - Milinković, Neda
AU  - Kortz, Urlich
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12942
AB  - Zahvaljujući brojnim istraživanjima koja su ispitivala biološka svojstva strukturno različitih polioksometalata, došlo se do zapažanja da ova kompleksna neorganska jedinjenja, pored antimikrobnog i antitumorskog delovanja, mogu biti delotvorna u snižavanju hiperglikemije kod pacova sa eksperimentalno izazvanim dijabetesom. Stoga, cilj ove studije je bio da se ispitaju antidijabetički potencijal i mogući toksični efekti dva polioksovolframata:(NH4 )14[NaP5 W30O110]·31H2 O, {NaP5 W30} i K14[AgP5 W30O110]·22H2 O·6KCl, {AgP5 W30}. U cilju realizacije postavljenog cilja, korišćena su tri eksperimentalna modela: (1) antihiperglikemijska screening studija u kojoj je ispitivan uticaj jednokratne intraperitonealne primene {NaP5 W30} i {AgP5 W30} (5, 10 i 20 mg/kg) na snižavanje hiperglikemije kod dijabetičkih pacova, (2) akutna peroralna toksikološka studija koja je istraživala hepato- i nefrotoksične efekte odabranih heteropolivolframata kod zdravih pacova i (3) studija posvećena rasvetljavanju mogućih mehanizama antidijabetičkog delovanja heteropolivolframata. Rezultati screening studije su pokazali da su oba ispitivana heteropolivolframata efikasna u snižavanju hiperglikemije, s tim što se {NaP5 W30}, u odnosu na {AgP5 W30}, pokazao kao moćniji antihiperglikemijski agens. Rezultati biohemijskih parametara funkcije i patohistološka analiza jetre i bubrege korišćenjem konvencionalne svetlosne i transmisione elektronske mikroskopije pokazuju da dvonedeljna primena {NaP5 W30} i {AgP5 W30} (20 mg/kg) izaziva blagi do umereni stepen hepato- i nefrotoksičnosti kod zdravih životinja. U poslednjem eksperimentalnom protokolu, pokazano je da tronedeljna peroralna primena {NaP5 W30} (20 mg/kg) povećava koncentraciju insulina u serumu dijabetičkih pacova, što može biti jedan od mehanizama njegovog antidijabetičkog delovanja. Takođe, pokazano je da {NaP5 W30} ispoljava hepato-, nefro-, kardio- i neuroprotektivno dejstvo kod dijabetičkih pacova, što je procenjeno na osnovu analize: (1) relativne mase organa, (2) biohemijskih parametara funkcije, (3) parametara oksidativnog stresa u homogenatu tkiva, (4) aktivnosti acetilholinesteraze, Na+ /K+-ATPaze i ecto-ATPaza u sinaptozomima i (5) patohistoloških promena u tkivima korišćenjem konvencionalne svetlosne i transmisione elektronske mikroskopije. Stoga, {NaP5 W30} i {AgP5 W30} mogu se smatrati mogućim neinsulinskim lekovima-kandidatima u terapiji dijabetesa tipa 2, koji bi se podvrgli daljim pretkliničkim istraživanjima.
C3  - Medical Research : Medicinska istraživanja
T1  - Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera
VL  - 56
IS  - 4
SP  - 132
EP  - 133
DO  - 10.5937/medi56-47579
ER  - 

@conference{
author = "Dinčić, Marko and Todorović, Jasna and Čolović, Mirjana and Ćetković Milisavljević, Mila and Kravić Stevović, Tamara and Milosavljević, Aleksandra and Milinković, Neda and Kortz, Urlich and Krstić, Danijela",
year = "2023",
abstract = "Zahvaljujući brojnim istraživanjima koja su ispitivala biološka svojstva strukturno različitih polioksometalata, došlo se do zapažanja da ova kompleksna neorganska jedinjenja, pored antimikrobnog i antitumorskog delovanja, mogu biti delotvorna u snižavanju hiperglikemije kod pacova sa eksperimentalno izazvanim dijabetesom. Stoga, cilj ove studije je bio da se ispitaju antidijabetički potencijal i mogući toksični efekti dva polioksovolframata:(NH4 )14[NaP5 W30O110]·31H2 O, {NaP5 W30} i K14[AgP5 W30O110]·22H2 O·6KCl, {AgP5 W30}. U cilju realizacije postavljenog cilja, korišćena su tri eksperimentalna modela: (1) antihiperglikemijska screening studija u kojoj je ispitivan uticaj jednokratne intraperitonealne primene {NaP5 W30} i {AgP5 W30} (5, 10 i 20 mg/kg) na snižavanje hiperglikemije kod dijabetičkih pacova, (2) akutna peroralna toksikološka studija koja je istraživala hepato- i nefrotoksične efekte odabranih heteropolivolframata kod zdravih pacova i (3) studija posvećena rasvetljavanju mogućih mehanizama antidijabetičkog delovanja heteropolivolframata. Rezultati screening studije su pokazali da su oba ispitivana heteropolivolframata efikasna u snižavanju hiperglikemije, s tim što se {NaP5 W30}, u odnosu na {AgP5 W30}, pokazao kao moćniji antihiperglikemijski agens. Rezultati biohemijskih parametara funkcije i patohistološka analiza jetre i bubrege korišćenjem konvencionalne svetlosne i transmisione elektronske mikroskopije pokazuju da dvonedeljna primena {NaP5 W30} i {AgP5 W30} (20 mg/kg) izaziva blagi do umereni stepen hepato- i nefrotoksičnosti kod zdravih životinja. U poslednjem eksperimentalnom protokolu, pokazano je da tronedeljna peroralna primena {NaP5 W30} (20 mg/kg) povećava koncentraciju insulina u serumu dijabetičkih pacova, što može biti jedan od mehanizama njegovog antidijabetičkog delovanja. Takođe, pokazano je da {NaP5 W30} ispoljava hepato-, nefro-, kardio- i neuroprotektivno dejstvo kod dijabetičkih pacova, što je procenjeno na osnovu analize: (1) relativne mase organa, (2) biohemijskih parametara funkcije, (3) parametara oksidativnog stresa u homogenatu tkiva, (4) aktivnosti acetilholinesteraze, Na+ /K+-ATPaze i ecto-ATPaza u sinaptozomima i (5) patohistoloških promena u tkivima korišćenjem konvencionalne svetlosne i transmisione elektronske mikroskopije. Stoga, {NaP5 W30} i {AgP5 W30} mogu se smatrati mogućim neinsulinskim lekovima-kandidatima u terapiji dijabetesa tipa 2, koji bi se podvrgli daljim pretkliničkim istraživanjima.",
journal = "Medical Research : Medicinska istraživanja",
title = "Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera",
volume = "56",
number = "4",
pages = "132-133",
doi = "10.5937/medi56-47579"
}

Dinčić, M., Todorović, J., Čolović, M., Ćetković Milisavljević, M., Kravić Stevović, T., Milosavljević, A., Milinković, N., Kortz, U.,& Krstić, D.. (2023). Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera. in Medical Research : Medicinska istraživanja, 56(4), 132-133.
https://doi.org/10.5937/medi56-47579

Dinčić M, Todorović J, Čolović M, Ćetković Milisavljević M, Kravić Stevović T, Milosavljević A, Milinković N, Kortz U, Krstić D. Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera. in Medical Research : Medicinska istraživanja. 2023;56(4):132-133.
doi:10.5937/medi56-47579 .

Dinčić, Marko, Todorović, Jasna, Čolović, Mirjana, Ćetković Milisavljević, Mila, Kravić Stevović, Tamara, Milosavljević, Aleksandra, Milinković, Neda, Kortz, Urlich, Krstić, Danijela, "Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera" in Medical Research : Medicinska istraživanja, 56, no. 4 (2023):132-133,
https://doi.org/10.5937/medi56-47579 . .

TY  - JOUR
AU  - Todorović, Jasna
AU  - Dinčić, Marko
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Nešović-Ostojić, Jelena
AU  - Kovačević, Sanjin
AU  - Lopičić, Srđan
AU  - Spasić, Svetolik
AU  - Brkić, Predrag
AU  - Milovanović, Aleksandar
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10745
AB  - Background: Thyroid dysfunctions as well as sleep abnormalities are usually followed by neurological, psychiatric and/or behavioral disorders. On the other hand, changes in the brain adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) activities show significant importance in pathogenetic pathways in the evolution of numerous neuropsychiatric diseases. Methods: This study aimed to evaluate the in vivo simultaneous effects of hypothyroidism and paradoxical sleep deprivation for 72 h on synaptosomalATPases and AChE activities of whole rat brains. In order to induce hypothyroidism, 6-n-propyl-2-thiouracil was administrated in drinking water during 21 days. The modified multiple platform method was used to induce paradoxical sleep deprivation. The AChE and ATPases activities were measured using spectrophotometric methods. Results: Hypothyroidism significantly increased the activity of Na+/K+-ATPase compared to other groups, while at the same time significantly decreased AChE activity compared to the CT and SD groups. Paradoxical sleep deprivation significantly increased AChE activity compared to other groups. The simultaneous effect of hypothyroidism and sleep deprivation reduced the activity of all three enzymes (for Na+/K+-ATPase between HT/SD and HT group p < 0.0001, SD group p < 0.001,CT group p = 0.013; for ecto-ATPases between HT/SD and HT group p = 0.0034, SD group p = 0.0001, CT group p = 0.0007; for AChE between HT/SD and HT group p < 0.05, SD group p < 0.0001, CT group p < 0.0001). Conclusions: The effect of simultaneous existence of hypothyroidism and paradoxical sleep deprivation reduces the activity of the Na+/K+-ATPase, ecto-ATPases, and AChE, what is different from individual effect of hypothyroidism and paradoxical sleep deprivation itself. This knowledge could help in the choice of appropriate therapy in such condition. © 2023 Elsevier B.V.
T2  - Sleep Medicine
T1  - The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain
VL  - 105
SP  - 14
EP  - 20
DO  - 10.1016/j.sleep.2023.03.002
ER  - 

@article{
author = "Todorović, Jasna and Dinčić, Marko and Krstić, Danijela Z. and Čolović, Mirjana B. and Nešović-Ostojić, Jelena and Kovačević, Sanjin and Lopičić, Srđan and Spasić, Svetolik and Brkić, Predrag and Milovanović, Aleksandar",
year = "2023",
abstract = "Background: Thyroid dysfunctions as well as sleep abnormalities are usually followed by neurological, psychiatric and/or behavioral disorders. On the other hand, changes in the brain adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) activities show significant importance in pathogenetic pathways in the evolution of numerous neuropsychiatric diseases. Methods: This study aimed to evaluate the in vivo simultaneous effects of hypothyroidism and paradoxical sleep deprivation for 72 h on synaptosomalATPases and AChE activities of whole rat brains. In order to induce hypothyroidism, 6-n-propyl-2-thiouracil was administrated in drinking water during 21 days. The modified multiple platform method was used to induce paradoxical sleep deprivation. The AChE and ATPases activities were measured using spectrophotometric methods. Results: Hypothyroidism significantly increased the activity of Na+/K+-ATPase compared to other groups, while at the same time significantly decreased AChE activity compared to the CT and SD groups. Paradoxical sleep deprivation significantly increased AChE activity compared to other groups. The simultaneous effect of hypothyroidism and sleep deprivation reduced the activity of all three enzymes (for Na+/K+-ATPase between HT/SD and HT group p < 0.0001, SD group p < 0.001,CT group p = 0.013; for ecto-ATPases between HT/SD and HT group p = 0.0034, SD group p = 0.0001, CT group p = 0.0007; for AChE between HT/SD and HT group p < 0.05, SD group p < 0.0001, CT group p < 0.0001). Conclusions: The effect of simultaneous existence of hypothyroidism and paradoxical sleep deprivation reduces the activity of the Na+/K+-ATPase, ecto-ATPases, and AChE, what is different from individual effect of hypothyroidism and paradoxical sleep deprivation itself. This knowledge could help in the choice of appropriate therapy in such condition. © 2023 Elsevier B.V.",
journal = "Sleep Medicine",
title = "The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain",
volume = "105",
pages = "14-20",
doi = "10.1016/j.sleep.2023.03.002"
}

Todorović, J., Dinčić, M., Krstić, D. Z., Čolović, M. B., Nešović-Ostojić, J., Kovačević, S., Lopičić, S., Spasić, S., Brkić, P.,& Milovanović, A.. (2023). The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain. in Sleep Medicine, 105, 14-20.
https://doi.org/10.1016/j.sleep.2023.03.002

Todorović J, Dinčić M, Krstić DZ, Čolović MB, Nešović-Ostojić J, Kovačević S, Lopičić S, Spasić S, Brkić P, Milovanović A. The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain. in Sleep Medicine. 2023;105:14-20.
doi:10.1016/j.sleep.2023.03.002 .

Todorović, Jasna, Dinčić, Marko, Krstić, Danijela Z., Čolović, Mirjana B., Nešović-Ostojić, Jelena, Kovačević, Sanjin, Lopičić, Srđan, Spasić, Svetolik, Brkić, Predrag, Milovanović, Aleksandar, "The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain" in Sleep Medicine, 105 (2023):14-20,
https://doi.org/10.1016/j.sleep.2023.03.002 . .

TY  - JOUR
AU  - Stojanović, Marko
AU  - Lalatović, Jovana
AU  - Milosavljević, Aleksandra
AU  - Savić, Nada
AU  - Simms, Charlotte
AU  - Radosavljević, Branimir
AU  - Ćetković, Mila
AU  - Kravić Stevović, Tamara
AU  - Mrda, Davor
AU  - Čolović, Mirjana B.
AU  - Parac-Vogt, Tatjana N.
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11091
AB  - In this study, we demonstrate for the first time, that a discrete metal-oxo cluster α-/β-K 6 P 2 W 18 O 62 (WD-POM) exhibits superior performance as a computed tomography (CT) contrast agent, in comparison to the standard contrast agent iohexol. A toxicity evaluation of WD-POM was performed according to standard toxicological protocols using Wistar albino rats. The maximum tolerable dose (MTD) of 2000 mg/kg was initially determined after oral WD-POM application. The acute intravenous toxicity of single WD-POM doses (1/3, 1/5, and 1/10 MTD), which are at least fifty times higher than the typically used dose (0.015 mmol W kg −1 ) of tungsten-based contrast agents, was evaluated for 14 days. The results of arterial blood gas analysis, CO-oximetry status, electrolyte and lactate levels for 1/10 MTD group (80% survival rate) indicated the mixed respiratory and metabolic acidosis. The highest deposition of WD-POM (0.6 ppm tungsten) was found in the kidney, followed by liver (0.15 ppm tungsten), for which the histological analysis revealed morphological irregularities, although the renal function parameters (creatinine and BUN levels) were within the physiological range. This study is the first and important step in evaluating side effects of polyoxometalate nanoclusters, which in recent years have shown a large potential as therapeutics and contrast agents.
T2  - Scientific Reports
T1  - In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography
VL  - 13
IS  - 1
SP  - 9140
DO  - 10.1038/s41598-023-36317-8
ER  - 

@article{
author = "Stojanović, Marko and Lalatović, Jovana and Milosavljević, Aleksandra and Savić, Nada and Simms, Charlotte and Radosavljević, Branimir and Ćetković, Mila and Kravić Stevović, Tamara and Mrda, Davor and Čolović, Mirjana B. and Parac-Vogt, Tatjana N. and Krstić, Danijela",
year = "2023",
abstract = "In this study, we demonstrate for the first time, that a discrete metal-oxo cluster α-/β-K 6 P 2 W 18 O 62 (WD-POM) exhibits superior performance as a computed tomography (CT) contrast agent, in comparison to the standard contrast agent iohexol. A toxicity evaluation of WD-POM was performed according to standard toxicological protocols using Wistar albino rats. The maximum tolerable dose (MTD) of 2000 mg/kg was initially determined after oral WD-POM application. The acute intravenous toxicity of single WD-POM doses (1/3, 1/5, and 1/10 MTD), which are at least fifty times higher than the typically used dose (0.015 mmol W kg −1 ) of tungsten-based contrast agents, was evaluated for 14 days. The results of arterial blood gas analysis, CO-oximetry status, electrolyte and lactate levels for 1/10 MTD group (80% survival rate) indicated the mixed respiratory and metabolic acidosis. The highest deposition of WD-POM (0.6 ppm tungsten) was found in the kidney, followed by liver (0.15 ppm tungsten), for which the histological analysis revealed morphological irregularities, although the renal function parameters (creatinine and BUN levels) were within the physiological range. This study is the first and important step in evaluating side effects of polyoxometalate nanoclusters, which in recent years have shown a large potential as therapeutics and contrast agents.",
journal = "Scientific Reports",
title = "In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography",
volume = "13",
number = "1",
pages = "9140",
doi = "10.1038/s41598-023-36317-8"
}

Stojanović, M., Lalatović, J., Milosavljević, A., Savić, N., Simms, C., Radosavljević, B., Ćetković, M., Kravić Stevović, T., Mrda, D., Čolović, M. B., Parac-Vogt, T. N.,& Krstić, D.. (2023). In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography. in Scientific Reports, 13(1), 9140.
https://doi.org/10.1038/s41598-023-36317-8

Stojanović M, Lalatović J, Milosavljević A, Savić N, Simms C, Radosavljević B, Ćetković M, Kravić Stevović T, Mrda D, Čolović MB, Parac-Vogt TN, Krstić D. In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography. in Scientific Reports. 2023;13(1):9140.
doi:10.1038/s41598-023-36317-8 .

Stojanović, Marko, Lalatović, Jovana, Milosavljević, Aleksandra, Savić, Nada, Simms, Charlotte, Radosavljević, Branimir, Ćetković, Mila, Kravić Stevović, Tamara, Mrda, Davor, Čolović, Mirjana B., Parac-Vogt, Tatjana N., Krstić, Danijela, "In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography" in Scientific Reports, 13, no. 1 (2023):9140,
https://doi.org/10.1038/s41598-023-36317-8 . .

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela
AU  - Vasić, Vesna M.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12242
AB  - Abstract: Bioassays based on the determination of acetylcholinesterase (AChE) activity are used for the detection and neurotoxicity evaluation of organophosphate insecticides. However, AChE bioassays are convenient for oxo and iso forms, whereas the limits of detection (LODs) of thio forms, being used as commercial insecticide preparations are significantly higher. In this study various malathion concentrations (1 × 10–9–5 × 10–4 mol/L) were treated with the oxidizing agent, N-bromosuccinimide (NBS) in concentration ratios: 1 : 1, 1 : 2, 1 : 10, and 1 : 20 to find efficient oxidation resulting in as possible as lower LODs of the used assays based on electric eel AChE (20 min-preincubation) and immobilized AChE incorporated in flow-injection analysis (FIA) system. Malathion–NBS ratio of 1 : 10 was found as the most efficient and resulted in a decrease of LOD about 100 times for both AChE bioassays. In the case of free AChE the obtained LOD values after the NBS-induced oxidation of thioorganophosphates (1 : 10), malathion, diazinon, and chlorpyrifos were as follows: 1.0 × 10–8, 1.3 × 10–8, and 1.0 × 10–8 mol/L, respectively. In addition, LOD values for the FIA-AChE system involving a pre-step with NBS induced the following LOD values: 7.2 × 10–7, 1.3 × 10–6, and 1.8 × 10–7 for malathion, diazinon, and chlorpyrifos, respectively. Furthermore, IC50 values of the corresponding oxo forms were found to be similar to those for the studied thioorganophosphates, which indicates a potential stoichiometric conversion of the thio to oxo forms under the established oxidation conditions.
T2  - Russian Journal of Physical Chemistry A
T1  - Oxidation Pretreatment to Improve the Sensitivity of Acetylcholinesterase-Based Detection of Thioorganophosphates
VL  - 97
IS  - 12
SP  - 2894
EP  - 2902
DO  - 10.1134/S0036024423120221
ER  - 

@article{
author = "Čolović, Mirjana B. and Krstić, Danijela and Vasić, Vesna M.",
year = "2023",
abstract = "Abstract: Bioassays based on the determination of acetylcholinesterase (AChE) activity are used for the detection and neurotoxicity evaluation of organophosphate insecticides. However, AChE bioassays are convenient for oxo and iso forms, whereas the limits of detection (LODs) of thio forms, being used as commercial insecticide preparations are significantly higher. In this study various malathion concentrations (1 × 10–9–5 × 10–4 mol/L) were treated with the oxidizing agent, N-bromosuccinimide (NBS) in concentration ratios: 1 : 1, 1 : 2, 1 : 10, and 1 : 20 to find efficient oxidation resulting in as possible as lower LODs of the used assays based on electric eel AChE (20 min-preincubation) and immobilized AChE incorporated in flow-injection analysis (FIA) system. Malathion–NBS ratio of 1 : 10 was found as the most efficient and resulted in a decrease of LOD about 100 times for both AChE bioassays. In the case of free AChE the obtained LOD values after the NBS-induced oxidation of thioorganophosphates (1 : 10), malathion, diazinon, and chlorpyrifos were as follows: 1.0 × 10–8, 1.3 × 10–8, and 1.0 × 10–8 mol/L, respectively. In addition, LOD values for the FIA-AChE system involving a pre-step with NBS induced the following LOD values: 7.2 × 10–7, 1.3 × 10–6, and 1.8 × 10–7 for malathion, diazinon, and chlorpyrifos, respectively. Furthermore, IC50 values of the corresponding oxo forms were found to be similar to those for the studied thioorganophosphates, which indicates a potential stoichiometric conversion of the thio to oxo forms under the established oxidation conditions.",
journal = "Russian Journal of Physical Chemistry A",
title = "Oxidation Pretreatment to Improve the Sensitivity of Acetylcholinesterase-Based Detection of Thioorganophosphates",
volume = "97",
number = "12",
pages = "2894-2902",
doi = "10.1134/S0036024423120221"
}

Čolović, M. B., Krstić, D.,& Vasić, V. M.. (2023). Oxidation Pretreatment to Improve the Sensitivity of Acetylcholinesterase-Based Detection of Thioorganophosphates. in Russian Journal of Physical Chemistry A, 97(12), 2894-2902.
https://doi.org/10.1134/S0036024423120221

Čolović MB, Krstić D, Vasić VM. Oxidation Pretreatment to Improve the Sensitivity of Acetylcholinesterase-Based Detection of Thioorganophosphates. in Russian Journal of Physical Chemistry A. 2023;97(12):2894-2902.
doi:10.1134/S0036024423120221 .

Čolović, Mirjana B., Krstić, Danijela, Vasić, Vesna M., "Oxidation Pretreatment to Improve the Sensitivity of Acetylcholinesterase-Based Detection of Thioorganophosphates" in Russian Journal of Physical Chemistry A, 97, no. 12 (2023):2894-2902,
https://doi.org/10.1134/S0036024423120221 . .

TY  - CONF
AU  - Čakar, Uroš
AU  - Čolović, Mirjana
AU  - Čebela, Maria
AU  - Petrović, Aleksandar
AU  - Krstić, Danijela
AU  - Stanković, Ivan
AU  - Đorđević, Brižita
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11491
AB  - Natural active principles are present in the fruit and during its processing pass into the final products. Among many natural active principles it is important to emphasize those which exhibit beneficial health effect on human organism. Beneficial health effect of those compounds is result of antioxidant properties. Wine is fruit derived product which is rich source of those compounds. The aim of this study was to investigate the influence of technological process on the phenolic profile, antioxidant properties and activity of antioxidant protection enzymes in vitro. Fruit wines were produced in controlled conditions during microvinifications. Phenolic profile of fruit wines were obtained by UPLC MS/MS, while antioxidant properties and enzymatic activity determined by spectrophotometric methods. The content of phenolic compounds in fruit wines was significant. It is important to highlight that different applied technology during the production, influenced on the content of above mentioned compounds. Flavonoids and phenolic acids were the most important. Also fruit wines showed ability to activate enzymes of antioxidant protection, which could be used in the protection against free radicals and prevention of oxidative stress.
PB  - Belgrade : University of Belgrade, Institute for Multidisciplinary Research
C3  - Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade
T1  - The Influence of Technological Process on the Content of Natural Active Principles From Fruit Wines and Its Beneficial Health Effects
SP  - 47
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11491
ER  - 

@conference{
author = "Čakar, Uroš and Čolović, Mirjana and Čebela, Maria and Petrović, Aleksandar and Krstić, Danijela and Stanković, Ivan and Đorđević, Brižita",
year = "2023",
abstract = "Natural active principles are present in the fruit and during its processing pass into the final products. Among many natural active principles it is important to emphasize those which exhibit beneficial health effect on human organism. Beneficial health effect of those compounds is result of antioxidant properties. Wine is fruit derived product which is rich source of those compounds. The aim of this study was to investigate the influence of technological process on the phenolic profile, antioxidant properties and activity of antioxidant protection enzymes in vitro. Fruit wines were produced in controlled conditions during microvinifications. Phenolic profile of fruit wines were obtained by UPLC MS/MS, while antioxidant properties and enzymatic activity determined by spectrophotometric methods. The content of phenolic compounds in fruit wines was significant. It is important to highlight that different applied technology during the production, influenced on the content of above mentioned compounds. Flavonoids and phenolic acids were the most important. Also fruit wines showed ability to activate enzymes of antioxidant protection, which could be used in the protection against free radicals and prevention of oxidative stress.",
publisher = "Belgrade : University of Belgrade, Institute for Multidisciplinary Research",
journal = "Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade",
title = "The Influence of Technological Process on the Content of Natural Active Principles From Fruit Wines and Its Beneficial Health Effects",
pages = "47-47",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11491"
}

Čakar, U., Čolović, M., Čebela, M., Petrović, A., Krstić, D., Stanković, I.,& Đorđević, B.. (2023). The Influence of Technological Process on the Content of Natural Active Principles From Fruit Wines and Its Beneficial Health Effects. in Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade
Belgrade : University of Belgrade, Institute for Multidisciplinary Research., 47-47.
https://hdl.handle.net/21.15107/rcub_vinar_11491

Čakar U, Čolović M, Čebela M, Petrović A, Krstić D, Stanković I, Đorđević B. The Influence of Technological Process on the Content of Natural Active Principles From Fruit Wines and Its Beneficial Health Effects. in Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade. 2023;:47-47.
https://hdl.handle.net/21.15107/rcub_vinar_11491 .

Čakar, Uroš, Čolović, Mirjana, Čebela, Maria, Petrović, Aleksandar, Krstić, Danijela, Stanković, Ivan, Đorđević, Brižita, "The Influence of Technological Process on the Content of Natural Active Principles From Fruit Wines and Its Beneficial Health Effects" in Programme and the Book of Abstracts / 7th Conference of The Serbian Society for Ceramic Materials, 7CSCS-2023, June 14-16, 2023 Belgrade (2023):47-47,
https://hdl.handle.net/21.15107/rcub_vinar_11491 .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Gajski, Goran
AU  - Domijan, Ana-Marija
AU  - Gerić, Marko
AU  - Savić, Nada
AU  - Parac-Vogt, Tatjana
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12229
AB  - Tungsten-based nanoparticles possess high density and capability to attenuate X-rays and thus have been studied as interesting candidates for the development of new-generation contrast-enhancing staining agents (CESAs) for computed tomography (CT). Polyoxotungstates, as polyoxoanions containing tungsten in its high oxidation state (W6+) were reported as promising CESA candidates to visualize long bones and kidney tissues. However, some polyoxotungstates induced side effects in toxicity studies in vivo, which could limit their clinical application. Thus, the aim of this study was to evaluate genotoxic effects in vitro of monolacunary Wells-Dawson polyoxotungstate, α2-K10P2W17O61.20H2O (lacunary WD) that was found as a potential contrast agent for CT in our previous studies in situ and in vitro. Lacunary WD was synthesized by following the reported procedure. The genotoxicity evaluation was performed by using the standard procedure for the alkaline comet assay. Briefly, human whole blood samples were taken from healthy male and female donors and exposed to different lacunary WD concentrations within the range of 10-6-10-4 mol/L, for 4 and 24 h at 37 °C. Then, 5 μL of whole blood was embedded into an agarose matrix and subsequently lysed (2.5 M NaCl, 100 mM EDTANa2, 10 mM Tris, 1% sodium sarcosinate, 1% Triton X-100, 10% DMSO, pH 10) overnight at 4 °C. After the lysis, the slides were placed into an alkaline solution (300 mM NaOH, 1 mM EDTANa2, pH 13) for 20 min at 4 °C to allow DNA unwinding and subsequently electrophoresed for 20 min at 1 V/cm. Finally, the slides were neutralized in 0.4 M Tris buffer (pH 7.5) for 5 min 3 times, stained with ethidium bromide (10 μg/mL), and analyzed at 250× magnification using an epifluorescence microscope (Zeiss, Göttingen, Germany) connected through a camera to an image analysis system (Comet Assay II; Perceptive Instruments Ltd., Haverhill, Suffolk, UK). One hundred randomly captured comets from each slide were examined. Multiple comparisons between groups were done by means of ANOVA on log-transformed data. Post hoc analyses of the differences were done by the Scheffé test. The percentage of tail DNA was determined to evaluate the level of DNA damage and genotoxicity potential. The obtained results showed that lacunary WD did not induce a statistically significant relative increase of tail DNA compared to the corresponding control at all investigated concentrations, after both 4 and 24 h exposure. Accordingly, the investigated promising contrast agent candidate could be regarded in further studies as toxicologically safe for healthy human blood cells from a genotoxicity point of view.
PB  - Niš : RAD Centre
C3  - RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts
T1  - In vitro genotoxicity assessment of a monolacunary Wells-Dawson nanocluster as a promising contrast agent candidate
SP  - 135
EP  - 135
DO  - 10.21175/rad.abstr.book.2023.23.3
ER  - 

@conference{
author = "Čolović, Mirjana and Gajski, Goran and Domijan, Ana-Marija and Gerić, Marko and Savić, Nada and Parac-Vogt, Tatjana and Krstić, Danijela",
year = "2023",
abstract = "Tungsten-based nanoparticles possess high density and capability to attenuate X-rays and thus have been studied as interesting candidates for the development of new-generation contrast-enhancing staining agents (CESAs) for computed tomography (CT). Polyoxotungstates, as polyoxoanions containing tungsten in its high oxidation state (W6+) were reported as promising CESA candidates to visualize long bones and kidney tissues. However, some polyoxotungstates induced side effects in toxicity studies in vivo, which could limit their clinical application. Thus, the aim of this study was to evaluate genotoxic effects in vitro of monolacunary Wells-Dawson polyoxotungstate, α2-K10P2W17O61.20H2O (lacunary WD) that was found as a potential contrast agent for CT in our previous studies in situ and in vitro. Lacunary WD was synthesized by following the reported procedure. The genotoxicity evaluation was performed by using the standard procedure for the alkaline comet assay. Briefly, human whole blood samples were taken from healthy male and female donors and exposed to different lacunary WD concentrations within the range of 10-6-10-4 mol/L, for 4 and 24 h at 37 °C. Then, 5 μL of whole blood was embedded into an agarose matrix and subsequently lysed (2.5 M NaCl, 100 mM EDTANa2, 10 mM Tris, 1% sodium sarcosinate, 1% Triton X-100, 10% DMSO, pH 10) overnight at 4 °C. After the lysis, the slides were placed into an alkaline solution (300 mM NaOH, 1 mM EDTANa2, pH 13) for 20 min at 4 °C to allow DNA unwinding and subsequently electrophoresed for 20 min at 1 V/cm. Finally, the slides were neutralized in 0.4 M Tris buffer (pH 7.5) for 5 min 3 times, stained with ethidium bromide (10 μg/mL), and analyzed at 250× magnification using an epifluorescence microscope (Zeiss, Göttingen, Germany) connected through a camera to an image analysis system (Comet Assay II; Perceptive Instruments Ltd., Haverhill, Suffolk, UK). One hundred randomly captured comets from each slide were examined. Multiple comparisons between groups were done by means of ANOVA on log-transformed data. Post hoc analyses of the differences were done by the Scheffé test. The percentage of tail DNA was determined to evaluate the level of DNA damage and genotoxicity potential. The obtained results showed that lacunary WD did not induce a statistically significant relative increase of tail DNA compared to the corresponding control at all investigated concentrations, after both 4 and 24 h exposure. Accordingly, the investigated promising contrast agent candidate could be regarded in further studies as toxicologically safe for healthy human blood cells from a genotoxicity point of view.",
publisher = "Niš : RAD Centre",
journal = "RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts",
title = "In vitro genotoxicity assessment of a monolacunary Wells-Dawson nanocluster as a promising contrast agent candidate",
pages = "135-135",
doi = "10.21175/rad.abstr.book.2023.23.3"
}

Čolović, M., Gajski, G., Domijan, A., Gerić, M., Savić, N., Parac-Vogt, T.,& Krstić, D.. (2023). In vitro genotoxicity assessment of a monolacunary Wells-Dawson nanocluster as a promising contrast agent candidate. in RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts
Niš : RAD Centre., 135-135.
https://doi.org/10.21175/rad.abstr.book.2023.23.3

Čolović M, Gajski G, Domijan A, Gerić M, Savić N, Parac-Vogt T, Krstić D. In vitro genotoxicity assessment of a monolacunary Wells-Dawson nanocluster as a promising contrast agent candidate. in RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts. 2023;:135-135.
doi:10.21175/rad.abstr.book.2023.23.3 .

Čolović, Mirjana, Gajski, Goran, Domijan, Ana-Marija, Gerić, Marko, Savić, Nada, Parac-Vogt, Tatjana, Krstić, Danijela, "In vitro genotoxicity assessment of a monolacunary Wells-Dawson nanocluster as a promising contrast agent candidate" in RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts (2023):135-135,
https://doi.org/10.21175/rad.abstr.book.2023.23.3 . .

TY  - JOUR
AU  - Wu, Zhikang
AU  - Dai, Guoyong
AU  - Li, Qi
AU  - Wei, Zheyu
AU  - Ru, Shi
AU  - Li, Jianda
AU  - Jia, Hongli
AU  - Zang, Dejin
AU  - Čolović, Mirjana
AU  - Wei, Yongge
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12920
AB  - The selective oxidative esterification of aldehydes with alcohols to the corresponding esters has been one of the hot spots in scientific research and industrial synthesis. However, the application of precious metal catalytic systems is limited by their complicated synthetic steps and high cost. Thus a highly efficient, green, recyclable selective synthesis method of esters catalyzed by POV(polyoxovanadate)-based molecular catalysts has been developed in this paper. The results show that supramolecular interaction between POV and 1,3-dibenzylimidazolium bromide (Act2Im) can efficiently convert alcohols and aldehydes to the corresponding esters in high yield under much milder conditions. Mechanistic insight is also provided based on the control experiments, single crystal X-ray diffraction and cyclic voltammetry studies.
T2  - Chinese Chemical Letters
T1  - POV-based molecular catalysts for highly efficient esterification of alcohols with aldehydes as acylating agents
IS  - InPress
SP  - 109061
DO  - 10.1016/j.cclet.2023.109061
ER  - 

@article{
author = "Wu, Zhikang and Dai, Guoyong and Li, Qi and Wei, Zheyu and Ru, Shi and Li, Jianda and Jia, Hongli and Zang, Dejin and Čolović, Mirjana and Wei, Yongge",
year = "2023",
abstract = "The selective oxidative esterification of aldehydes with alcohols to the corresponding esters has been one of the hot spots in scientific research and industrial synthesis. However, the application of precious metal catalytic systems is limited by their complicated synthetic steps and high cost. Thus a highly efficient, green, recyclable selective synthesis method of esters catalyzed by POV(polyoxovanadate)-based molecular catalysts has been developed in this paper. The results show that supramolecular interaction between POV and 1,3-dibenzylimidazolium bromide (Act2Im) can efficiently convert alcohols and aldehydes to the corresponding esters in high yield under much milder conditions. Mechanistic insight is also provided based on the control experiments, single crystal X-ray diffraction and cyclic voltammetry studies.",
journal = "Chinese Chemical Letters",
title = "POV-based molecular catalysts for highly efficient esterification of alcohols with aldehydes as acylating agents",
number = "InPress",
pages = "109061",
doi = "10.1016/j.cclet.2023.109061"
}

Wu, Z., Dai, G., Li, Q., Wei, Z., Ru, S., Li, J., Jia, H., Zang, D., Čolović, M.,& Wei, Y.. (2023). POV-based molecular catalysts for highly efficient esterification of alcohols with aldehydes as acylating agents. in Chinese Chemical Letters(InPress), 109061.
https://doi.org/10.1016/j.cclet.2023.109061

Wu Z, Dai G, Li Q, Wei Z, Ru S, Li J, Jia H, Zang D, Čolović M, Wei Y. POV-based molecular catalysts for highly efficient esterification of alcohols with aldehydes as acylating agents. in Chinese Chemical Letters. 2023;(InPress):109061.
doi:10.1016/j.cclet.2023.109061 .

Wu, Zhikang, Dai, Guoyong, Li, Qi, Wei, Zheyu, Ru, Shi, Li, Jianda, Jia, Hongli, Zang, Dejin, Čolović, Mirjana, Wei, Yongge, "POV-based molecular catalysts for highly efficient esterification of alcohols with aldehydes as acylating agents" in Chinese Chemical Letters, no. InPress (2023):109061,
https://doi.org/10.1016/j.cclet.2023.109061 . .

TY  - CONF
AU  - Domijan, Ana-Marija
AU  - Gajski, Goran
AU  - Gerić, M.
AU  - Savić, Nada
AU  - Parac-Vogt, Tatjana
AU  - Krstić, Danijela
AU  - Čolović, Mirjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12926
AB  - In this study, prooxidative properties of three Wells-Dawson polyoxometalates (POMs) previously reported as promising contrast-enhancing staining agents for micro-computed tomography (microCT): parent Wells-Dawson POM α2-K6P2W18O62.14H2O (parent WD), monolacunary Wells-Dawson POM α2-K10P2W17O61.20H2O (lacunary WD), and 1:2 hafnium(IV)-substituted Wells-Dawson POM K16[Hf(α2-P2W17O61)2]·19H2O (Hf-WD 1:2), were evaluated in vitro. In this aim, malondialdehyde (MDA) level, as an indicator of prooxidant action and lipid peroxidation, was determined in human plasma after 24 h treatment with the POMs in the concentration range 1-100 μM at 37 °C. The results demonstrated that the studied POMs did not induce an increase in plasma lipid peroxidation level compared to the corresponding control. Thus, it could be concluded that the investigated POM-based contrast agent candidates for microCT are not sufficiently potent prooxidants capable to cause membrane damage and consequent toxic occurrences.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings
T1  - In vitro toxicity evaluation of potential contrast agents for micro-computed tomography: effect on cell membrane
VL  - 1
SP  - 283
EP  - 286
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12926
ER  - 

@conference{
author = "Domijan, Ana-Marija and Gajski, Goran and Gerić, M. and Savić, Nada and Parac-Vogt, Tatjana and Krstić, Danijela and Čolović, Mirjana",
year = "2022",
abstract = "In this study, prooxidative properties of three Wells-Dawson polyoxometalates (POMs) previously reported as promising contrast-enhancing staining agents for micro-computed tomography (microCT): parent Wells-Dawson POM α2-K6P2W18O62.14H2O (parent WD), monolacunary Wells-Dawson POM α2-K10P2W17O61.20H2O (lacunary WD), and 1:2 hafnium(IV)-substituted Wells-Dawson POM K16[Hf(α2-P2W17O61)2]·19H2O (Hf-WD 1:2), were evaluated in vitro. In this aim, malondialdehyde (MDA) level, as an indicator of prooxidant action and lipid peroxidation, was determined in human plasma after 24 h treatment with the POMs in the concentration range 1-100 μM at 37 °C. The results demonstrated that the studied POMs did not induce an increase in plasma lipid peroxidation level compared to the corresponding control. Thus, it could be concluded that the investigated POM-based contrast agent candidates for microCT are not sufficiently potent prooxidants capable to cause membrane damage and consequent toxic occurrences.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings",
title = "In vitro toxicity evaluation of potential contrast agents for micro-computed tomography: effect on cell membrane",
volume = "1",
pages = "283-286",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12926"
}

Domijan, A., Gajski, G., Gerić, M., Savić, N., Parac-Vogt, T., Krstić, D.,& Čolović, M.. (2022). In vitro toxicity evaluation of potential contrast agents for micro-computed tomography: effect on cell membrane. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings
Belgrade : Society of Physical Chemists of Serbia., 1, 283-286.
https://hdl.handle.net/21.15107/rcub_vinar_12926

Domijan A, Gajski G, Gerić M, Savić N, Parac-Vogt T, Krstić D, Čolović M. In vitro toxicity evaluation of potential contrast agents for micro-computed tomography: effect on cell membrane. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings. 2022;1:283-286.
https://hdl.handle.net/21.15107/rcub_vinar_12926 .

Domijan, Ana-Marija, Gajski, Goran, Gerić, M., Savić, Nada, Parac-Vogt, Tatjana, Krstić, Danijela, Čolović, Mirjana, "In vitro toxicity evaluation of potential contrast agents for micro-computed tomography: effect on cell membrane" in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings, 1 (2022):283-286,
https://hdl.handle.net/21.15107/rcub_vinar_12926 .

TY  - CONF
AU  - Čakar, Uroš
AU  - Čolović, Mirjana
AU  - Čebela, Maria
AU  - Petrović, Aleksandar
AU  - Krstić, Danijela
AU  - Stanković, Ivan
AU  - Đorđević, Brižita
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11507
AB  - Fruit and derived product represent a rich source of biologically active compounds which exhibit beneficial health effect on human organism. Among fruit especially berries it is important to highlight blueberries. One of derived products with added value from this fruit is wine. The aim of this study was to investigate in vitro activity of blueberry wine by monitoring activities of antioxidant protection enzymes and lipid peroxidation (malondialdehyde level) in isolated rat synaptosomes. Fruit wines were produced in controlled conditions of different microvinifications in which pure culture of selected wine yeast was used. Synaptosomes were isolated from the brain of Wistar albino rats. Analyzed wine samples influenced on the activity of antioxidant protection enzymes. Wine samples also showed ability to decrease malondialdehyde level. Activity for superoxide dismutase in synaptosomes was in range (6.47–7.21 U/mg) while catalase activity was (0.045–0.061 U/mg). Glutathione peroxidase activity was in range (0.0212– 0.0232 U/mg), as well as malondialdehyde level (2.17–2.35 nmol/mg). Obtained results indicate that blueberry wines possess antioxidant properties and abilities to protect against free radicals generated during oxidative stress.
PB  - University of Belgrade : Institute for Multidisciplinary Research
C3  - 6CSCS : 6th Serbian Ceramic Society Conference : programme and the book of abstracts; June 28-29; Belgrade
T1  - Blueberry wine biologically active compounds protect against oxidative stress
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11507
ER  - 

@conference{
author = "Čakar, Uroš and Čolović, Mirjana and Čebela, Maria and Petrović, Aleksandar and Krstić, Danijela and Stanković, Ivan and Đorđević, Brižita",
year = "2022",
abstract = "Fruit and derived product represent a rich source of biologically active compounds which exhibit beneficial health effect on human organism. Among fruit especially berries it is important to highlight blueberries. One of derived products with added value from this fruit is wine. The aim of this study was to investigate in vitro activity of blueberry wine by monitoring activities of antioxidant protection enzymes and lipid peroxidation (malondialdehyde level) in isolated rat synaptosomes. Fruit wines were produced in controlled conditions of different microvinifications in which pure culture of selected wine yeast was used. Synaptosomes were isolated from the brain of Wistar albino rats. Analyzed wine samples influenced on the activity of antioxidant protection enzymes. Wine samples also showed ability to decrease malondialdehyde level. Activity for superoxide dismutase in synaptosomes was in range (6.47–7.21 U/mg) while catalase activity was (0.045–0.061 U/mg). Glutathione peroxidase activity was in range (0.0212– 0.0232 U/mg), as well as malondialdehyde level (2.17–2.35 nmol/mg). Obtained results indicate that blueberry wines possess antioxidant properties and abilities to protect against free radicals generated during oxidative stress.",
publisher = "University of Belgrade : Institute for Multidisciplinary Research",
journal = "6CSCS : 6th Serbian Ceramic Society Conference : programme and the book of abstracts; June 28-29; Belgrade",
title = "Blueberry wine biologically active compounds protect against oxidative stress",
pages = "65",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11507"
}

Čakar, U., Čolović, M., Čebela, M., Petrović, A., Krstić, D., Stanković, I.,& Đorđević, B.. (2022). Blueberry wine biologically active compounds protect against oxidative stress. in 6CSCS : 6th Serbian Ceramic Society Conference : programme and the book of abstracts; June 28-29; Belgrade
University of Belgrade : Institute for Multidisciplinary Research., 65.
https://hdl.handle.net/21.15107/rcub_vinar_11507

Čakar U, Čolović M, Čebela M, Petrović A, Krstić D, Stanković I, Đorđević B. Blueberry wine biologically active compounds protect against oxidative stress. in 6CSCS : 6th Serbian Ceramic Society Conference : programme and the book of abstracts; June 28-29; Belgrade. 2022;:65.
https://hdl.handle.net/21.15107/rcub_vinar_11507 .

Čakar, Uroš, Čolović, Mirjana, Čebela, Maria, Petrović, Aleksandar, Krstić, Danijela, Stanković, Ivan, Đorđević, Brižita, "Blueberry wine biologically active compounds protect against oxidative stress" in 6CSCS : 6th Serbian Ceramic Society Conference : programme and the book of abstracts; June 28-29; Belgrade (2022):65,
https://hdl.handle.net/21.15107/rcub_vinar_11507 .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Gajski, Goran
AU  - Gerić, M.
AU  - Savić, Nada
AU  - Krstić, Danijela
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12924
AB  - Cytotoxicity assessment in vitro of three Wells-Dawson polyoxometalates (POMs) as contrast-enhancing staining agent (CESA) candidates: parent Wells-Dawson POM α2-K6P2W18O62.14H2O (parent WD), monolacunary Wells-Dawson POM α2-K10P2W17O61.20H2O (lacunary WD), and 1:2 hafnium(IV)-substituted Wells-Dawson POM K16[Hf(α2-P2W17O61)2]·19H2O (Hf-WD 1:2), was carried out on healthy non-target human blood cells. Whole blood samples were treated with these POMs (1-100 μM) for 4 and 24 h at 37 °C. The results showed a time-dependent decrease of the peripheral blood mononuclear cell (PBMC) viability for all investigated polyoxotungstates. The 4 h treatment induced a significant cytotoxic effect only for the higher concentrations of Hf-WD 1:2, whereas 24 h exposure resulted in a concentration-dependent alteration of the cell viability. The most potent cytotoxic activity was obtained for Hf-WD 1:2 inducing a decrease of the cell viability from 25 to 45% compared to the corresponding control. The weakest effect was observed for parent WD, which resulted in no statistically significant difference in PBMC viability in respect to the control at the concentration of 1 μM. Finally, parent WD, lacunary WD, and Hf-WD 1:2 could not be considered safe from a cytotoxic point of view, thus further more extensive toxicity studies are needed.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings
T1  - Cytotoxicity evaluation of promising staining agents for contrast-enhanced microfocus computed tomography
VL  - 1
SP  - 275
EP  - 278
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12924
ER  - 

@conference{
author = "Čolović, Mirjana and Gajski, Goran and Gerić, M. and Savić, Nada and Krstić, Danijela",
year = "2022",
abstract = "Cytotoxicity assessment in vitro of three Wells-Dawson polyoxometalates (POMs) as contrast-enhancing staining agent (CESA) candidates: parent Wells-Dawson POM α2-K6P2W18O62.14H2O (parent WD), monolacunary Wells-Dawson POM α2-K10P2W17O61.20H2O (lacunary WD), and 1:2 hafnium(IV)-substituted Wells-Dawson POM K16[Hf(α2-P2W17O61)2]·19H2O (Hf-WD 1:2), was carried out on healthy non-target human blood cells. Whole blood samples were treated with these POMs (1-100 μM) for 4 and 24 h at 37 °C. The results showed a time-dependent decrease of the peripheral blood mononuclear cell (PBMC) viability for all investigated polyoxotungstates. The 4 h treatment induced a significant cytotoxic effect only for the higher concentrations of Hf-WD 1:2, whereas 24 h exposure resulted in a concentration-dependent alteration of the cell viability. The most potent cytotoxic activity was obtained for Hf-WD 1:2 inducing a decrease of the cell viability from 25 to 45% compared to the corresponding control. The weakest effect was observed for parent WD, which resulted in no statistically significant difference in PBMC viability in respect to the control at the concentration of 1 μM. Finally, parent WD, lacunary WD, and Hf-WD 1:2 could not be considered safe from a cytotoxic point of view, thus further more extensive toxicity studies are needed.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings",
title = "Cytotoxicity evaluation of promising staining agents for contrast-enhanced microfocus computed tomography",
volume = "1",
pages = "275-278",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12924"
}

Čolović, M., Gajski, G., Gerić, M., Savić, N.,& Krstić, D.. (2022). Cytotoxicity evaluation of promising staining agents for contrast-enhanced microfocus computed tomography. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings
Belgrade : Society of Physical Chemists of Serbia., 1, 275-278.
https://hdl.handle.net/21.15107/rcub_vinar_12924

Čolović M, Gajski G, Gerić M, Savić N, Krstić D. Cytotoxicity evaluation of promising staining agents for contrast-enhanced microfocus computed tomography. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings. 2022;1:275-278.
https://hdl.handle.net/21.15107/rcub_vinar_12924 .

Čolović, Mirjana, Gajski, Goran, Gerić, M., Savić, Nada, Krstić, Danijela, "Cytotoxicity evaluation of promising staining agents for contrast-enhanced microfocus computed tomography" in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings, 1 (2022):275-278,
https://hdl.handle.net/21.15107/rcub_vinar_12924 .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Domijan, Ana-Marija
AU  - Gajski, Goran
AU  - Gerić, M.
AU  - Parac-Vogt, Tatjana
AU  - Krstić, Danijela
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12925
AB  - The aim of this study to assess in vitro prooxidative properties of three polyoxometalate (POM)-based species that were found as potential contrast-enhancing staining agents for micro-computed tomography (microCT) to visualize kidney and bone tissues: parent Wells-Dawson POM α2-K6P2W18O62.14H2O (parent WD), monolacunary Wells-Dawson POM α2-K10P2W17O61.20H2O (lacunary WD), and 1:2 hafnium(IV)-substituted Wells-Dawson POM K16[Hf(α2-P2W17O61)2]·19H2O (Hf-WD 1:2). Thus, reduced glutathione (GSH) concentration, as a major antioxidant to prevent reactive oxygen species (ROS) attack and an indicator of oxidative stress, was followed in human plasma after 24 h exposure to the POMs within the concentration range 1-100 μM at 37 °C. The promising contrast agents exhibited various prooxidative properties. Parent WD did not significantly influence GSH level, thus prooxidative properties could not be associated with this POM species. On the contrary, Hf-WD 1:2 exposure resulted in a significant decrease in GSH concentration in human plasma compared to untreated control. Accordingly, Hf-WD 1:2 might be regarded as a prooxidant agent that could cause oxidative stress and consequent toxic occurrences. Nevertheless, additional studies on other relevant oxidative stress parameters are needed to confirm this assumption.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings
T1  - Prooxidative properties of contrast agent candidates for micro-computed tomography
VL  - 1
SP  - 279
EP  - 282
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12925
ER  - 

@conference{
author = "Čolović, Mirjana and Domijan, Ana-Marija and Gajski, Goran and Gerić, M. and Parac-Vogt, Tatjana and Krstić, Danijela",
year = "2022",
abstract = "The aim of this study to assess in vitro prooxidative properties of three polyoxometalate (POM)-based species that were found as potential contrast-enhancing staining agents for micro-computed tomography (microCT) to visualize kidney and bone tissues: parent Wells-Dawson POM α2-K6P2W18O62.14H2O (parent WD), monolacunary Wells-Dawson POM α2-K10P2W17O61.20H2O (lacunary WD), and 1:2 hafnium(IV)-substituted Wells-Dawson POM K16[Hf(α2-P2W17O61)2]·19H2O (Hf-WD 1:2). Thus, reduced glutathione (GSH) concentration, as a major antioxidant to prevent reactive oxygen species (ROS) attack and an indicator of oxidative stress, was followed in human plasma after 24 h exposure to the POMs within the concentration range 1-100 μM at 37 °C. The promising contrast agents exhibited various prooxidative properties. Parent WD did not significantly influence GSH level, thus prooxidative properties could not be associated with this POM species. On the contrary, Hf-WD 1:2 exposure resulted in a significant decrease in GSH concentration in human plasma compared to untreated control. Accordingly, Hf-WD 1:2 might be regarded as a prooxidant agent that could cause oxidative stress and consequent toxic occurrences. Nevertheless, additional studies on other relevant oxidative stress parameters are needed to confirm this assumption.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings",
title = "Prooxidative properties of contrast agent candidates for micro-computed tomography",
volume = "1",
pages = "279-282",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12925"
}

Čolović, M., Domijan, A., Gajski, G., Gerić, M., Parac-Vogt, T.,& Krstić, D.. (2022). Prooxidative properties of contrast agent candidates for micro-computed tomography. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings
Belgrade : Society of Physical Chemists of Serbia., 1, 279-282.
https://hdl.handle.net/21.15107/rcub_vinar_12925

Čolović M, Domijan A, Gajski G, Gerić M, Parac-Vogt T, Krstić D. Prooxidative properties of contrast agent candidates for micro-computed tomography. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings. 2022;1:279-282.
https://hdl.handle.net/21.15107/rcub_vinar_12925 .

Čolović, Mirjana, Domijan, Ana-Marija, Gajski, Goran, Gerić, M., Parac-Vogt, Tatjana, Krstić, Danijela, "Prooxidative properties of contrast agent candidates for micro-computed tomography" in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings, 1 (2022):279-282,
https://hdl.handle.net/21.15107/rcub_vinar_12925 .

TY  - CONF
AU  - Čolović, Mirjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12921
AB  - Cytotoxicity and prooxidative properties evaluation of three Wells-Dawson polyoxotungstates that were published as potential contrast-enhancing staining agent (CESA) candidates for micro-computed tomography (microCT): parent Wells-Dawson POM α2-K6P2W18O62.14H2O (parent WD), monolacunary Wells-Dawson POM α2-K10P2W17O61.20H2O (lacunary WD), and 1:2 hafnium(IV)-substituted Wells-Dawson POM K16[Hf(α2-P2W17O61)2]·19H2O (Hf-WD 1:2), was carried out in vitro. Oxidative stress indicators, reduced glutathione (GSH) and malondialdehyde (MDA) levels were determined in human blood plasma after 4 and 24 h treatment with the selected polyoxotungstates, within the concentration range 1-100 μM at 37 °C, and polyoxotungstate-induced cytotoxicity was assessed by monitoring cell viability in peripheral blood mononuclear cells (PBMCs). The results demonstrated that each of the studied promising CESAs resulted in a decrease of human PBMC viability in concentration- and time-dependent manner, and Hf-WD 1:2 exhibited the strongest cytotoxic properties inducing up to 50% decrease of the cell viability related to corresponding control. However, only Hf-WD 1:2 might be considered a potential prooxidant that resulted in a significant decrease of human plasma GSH level, whereas none of the investigated polyoxotungstates did not cause membrane lipid damage. Conclusively, parent WD, lacunary WD, and Hf-WD 1:2 could not be considered safe primarily from a cytotoxic point of view, thus more extensive in vitro and in vivo studies are needed for the toxicological profile assessment of the studied promising CESA candidates for the soft tissue visualization using microCT.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings
T1  - Toxicity evaluation of promising contrast-enhancing staining agents
VL  - 2
SP  - 573
EP  - 576
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12921
ER  - 

@conference{
author = "Čolović, Mirjana",
year = "2022",
abstract = "Cytotoxicity and prooxidative properties evaluation of three Wells-Dawson polyoxotungstates that were published as potential contrast-enhancing staining agent (CESA) candidates for micro-computed tomography (microCT): parent Wells-Dawson POM α2-K6P2W18O62.14H2O (parent WD), monolacunary Wells-Dawson POM α2-K10P2W17O61.20H2O (lacunary WD), and 1:2 hafnium(IV)-substituted Wells-Dawson POM K16[Hf(α2-P2W17O61)2]·19H2O (Hf-WD 1:2), was carried out in vitro. Oxidative stress indicators, reduced glutathione (GSH) and malondialdehyde (MDA) levels were determined in human blood plasma after 4 and 24 h treatment with the selected polyoxotungstates, within the concentration range 1-100 μM at 37 °C, and polyoxotungstate-induced cytotoxicity was assessed by monitoring cell viability in peripheral blood mononuclear cells (PBMCs). The results demonstrated that each of the studied promising CESAs resulted in a decrease of human PBMC viability in concentration- and time-dependent manner, and Hf-WD 1:2 exhibited the strongest cytotoxic properties inducing up to 50% decrease of the cell viability related to corresponding control. However, only Hf-WD 1:2 might be considered a potential prooxidant that resulted in a significant decrease of human plasma GSH level, whereas none of the investigated polyoxotungstates did not cause membrane lipid damage. Conclusively, parent WD, lacunary WD, and Hf-WD 1:2 could not be considered safe primarily from a cytotoxic point of view, thus more extensive in vitro and in vivo studies are needed for the toxicological profile assessment of the studied promising CESA candidates for the soft tissue visualization using microCT.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings",
title = "Toxicity evaluation of promising contrast-enhancing staining agents",
volume = "2",
pages = "573-576",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12921"
}

Čolović, M.. (2022). Toxicity evaluation of promising contrast-enhancing staining agents. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings
Belgrade : Society of Physical Chemists of Serbia., 2, 573-576.
https://hdl.handle.net/21.15107/rcub_vinar_12921

Čolović M. Toxicity evaluation of promising contrast-enhancing staining agents. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings. 2022;2:573-576.
https://hdl.handle.net/21.15107/rcub_vinar_12921 .

Čolović, Mirjana, "Toxicity evaluation of promising contrast-enhancing staining agents" in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Proceedings, 2 (2022):573-576,
https://hdl.handle.net/21.15107/rcub_vinar_12921 .

TY  - CONF
AU  - Čolović, Mirjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12922
AB  - Acetylcholinesterase is a serine hydrolase whose key biological role is the termination of impulse transmission at cholinergic synapses by rapid hydrolysis of the neurotransmitter acetylcholine. The reversible inhibition of brain acetylcholinesterase is the major therapeutic target in the treatment of Alzheimer’s disease associated with loss of cholinergic neurons in the brain and the decreased level of acetylcholine, whereas toxic effects are related to irreversible modulators of the enzyme activity. Na+/K+-ATPase is a transmembrane protein regulating many cellular functions, involving those associated with tumor cell growth. In addition, particular Na+/K+-ATPase subunits are expressed in some cancer cells and changes in Na+/K+-ATPase activity and relative subunit abundance were detected in various carcinoma cell lines. Accordingly, design and synthesis of novel compounds have been directed towards new modulators of Na+/K+-ATPase, which selectively target these cellular abnormalities. Ecto-nucleoside triphosphate diphosphohydrolases (ENTPDases) are plasma membrane bound enzymes representing the major part of purinergic signaling. Increased E-NTPDases levels were observed in cancer cells due to their abnormal cellular growth and proliferation. Accordingly, the decrease of E-NTPDase activity could be regarded as a new approach in the development of antitumor drugs. This presentation will be focused on metal-based compounds such as polyoxometalates which are discrete, negatively charged metal-oxo clusters of early d-block metal ions in high oxidation states, surrounded by oxygen atoms. These compounds were approved to exhibit a variety of biological actions such as anticancer, antimicrobial and antidiabetic properties. However, the mechanism of their bioactivities has not been completely understood yet. It has been assumed that polyoxometalates interact with different enzyme families extracellularly located on the plasma membrane such as phosphatases and ecto-nucleotidases. This presentation will primarily be directed to acetylcholinesterase, Na+/K+-ATPase and E-NTPDases as potential targets of polyoxometalate pharmacological and toxicological activities.
C3  - 7th Edition of Global Conference on Pharmaceutics and Novel Drug Delivery Systems : PDSS 2022 : Book of abstracts
T1  - Acetylcholinesterase and ATPases: targets of biologically active compounds
SP  - 14
EP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12922
ER  - 

@conference{
author = "Čolović, Mirjana",
year = "2022",
abstract = "Acetylcholinesterase is a serine hydrolase whose key biological role is the termination of impulse transmission at cholinergic synapses by rapid hydrolysis of the neurotransmitter acetylcholine. The reversible inhibition of brain acetylcholinesterase is the major therapeutic target in the treatment of Alzheimer’s disease associated with loss of cholinergic neurons in the brain and the decreased level of acetylcholine, whereas toxic effects are related to irreversible modulators of the enzyme activity. Na+/K+-ATPase is a transmembrane protein regulating many cellular functions, involving those associated with tumor cell growth. In addition, particular Na+/K+-ATPase subunits are expressed in some cancer cells and changes in Na+/K+-ATPase activity and relative subunit abundance were detected in various carcinoma cell lines. Accordingly, design and synthesis of novel compounds have been directed towards new modulators of Na+/K+-ATPase, which selectively target these cellular abnormalities. Ecto-nucleoside triphosphate diphosphohydrolases (ENTPDases) are plasma membrane bound enzymes representing the major part of purinergic signaling. Increased E-NTPDases levels were observed in cancer cells due to their abnormal cellular growth and proliferation. Accordingly, the decrease of E-NTPDase activity could be regarded as a new approach in the development of antitumor drugs. This presentation will be focused on metal-based compounds such as polyoxometalates which are discrete, negatively charged metal-oxo clusters of early d-block metal ions in high oxidation states, surrounded by oxygen atoms. These compounds were approved to exhibit a variety of biological actions such as anticancer, antimicrobial and antidiabetic properties. However, the mechanism of their bioactivities has not been completely understood yet. It has been assumed that polyoxometalates interact with different enzyme families extracellularly located on the plasma membrane such as phosphatases and ecto-nucleotidases. This presentation will primarily be directed to acetylcholinesterase, Na+/K+-ATPase and E-NTPDases as potential targets of polyoxometalate pharmacological and toxicological activities.",
journal = "7th Edition of Global Conference on Pharmaceutics and Novel Drug Delivery Systems : PDSS 2022 : Book of abstracts",
title = "Acetylcholinesterase and ATPases: targets of biologically active compounds",
pages = "14-14",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12922"
}

Čolović, M.. (2022). Acetylcholinesterase and ATPases: targets of biologically active compounds. in 7th Edition of Global Conference on Pharmaceutics and Novel Drug Delivery Systems : PDSS 2022 : Book of abstracts, 14-14.
https://hdl.handle.net/21.15107/rcub_vinar_12922

Čolović M. Acetylcholinesterase and ATPases: targets of biologically active compounds. in 7th Edition of Global Conference on Pharmaceutics and Novel Drug Delivery Systems : PDSS 2022 : Book of abstracts. 2022;:14-14.
https://hdl.handle.net/21.15107/rcub_vinar_12922 .

Čolović, Mirjana, "Acetylcholinesterase and ATPases: targets of biologically active compounds" in 7th Edition of Global Conference on Pharmaceutics and Novel Drug Delivery Systems : PDSS 2022 : Book of abstracts (2022):14-14,
https://hdl.handle.net/21.15107/rcub_vinar_12922 .

TY  - JOUR
AU  - Čakar, Uroš
AU  - Čolović, Mirjana B.
AU  - Milenković, Danijela
AU  - Medić, Branislava
AU  - Krstić, Danijela Z.
AU  - Petrović, Aleksandar
AU  - Ðoređvić, Brižita
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9889
AB  - This study aimed to evaluate, in vitro, the antioxidative potential of fruit wines produced from berry fruits (i.e., black chokeberry, blueberry, blackberry, and raspberry), cherry, and apple by different technological processes. For this purpose, the activities of antioxidant enzymes (catalase, glutathione peroxidase (GPx), and superoxide dismutase (SOD)) and malondialdehyde (MDA) content as a marker of membrane damage were determined in wine-treated synaptosomes with hydrogen peroxide-induced oxidative stress. All studied wines induced increased antioxidant enzyme activities and decreased MDA levels compared to hydrogen peroxide-treated synaptosomes (i.e., control). The highest SOD activity was observed in synaptosomes treated with blackberry wine (6.81 U/mg), whereas blueberry wine induced the highest catalase and glutathione peroxidase activities (0.058 U/mg and 0.017 U/mg, respectively). Black chokeberry proved to be the best in lipid peroxidation protection with the lowest MDA value (1.42 nmol/mg). Finally, principal component analysis and hierarchical cluster analysis additionally highlighted a higher antioxidant capacity of wines produced from dark-skinned fruits (i.e., blackberry, black chokeberry, and blueberry). The results suggest protective effects of the fruit wines against oxidative damage, and, accordingly, their promising application as functional food.
T2  - Agronomy
T1  - Protective effects of fruit wines against hydrogen peroxide—induced oxidative stress in rat synaptosomes
VL  - 11
IS  - 7
SP  - 1414
DO  - 10.3390/agronomy11071414
ER  - 

@article{
author = "Čakar, Uroš and Čolović, Mirjana B. and Milenković, Danijela and Medić, Branislava and Krstić, Danijela Z. and Petrović, Aleksandar and Ðoređvić, Brižita",
year = "2021",
abstract = "This study aimed to evaluate, in vitro, the antioxidative potential of fruit wines produced from berry fruits (i.e., black chokeberry, blueberry, blackberry, and raspberry), cherry, and apple by different technological processes. For this purpose, the activities of antioxidant enzymes (catalase, glutathione peroxidase (GPx), and superoxide dismutase (SOD)) and malondialdehyde (MDA) content as a marker of membrane damage were determined in wine-treated synaptosomes with hydrogen peroxide-induced oxidative stress. All studied wines induced increased antioxidant enzyme activities and decreased MDA levels compared to hydrogen peroxide-treated synaptosomes (i.e., control). The highest SOD activity was observed in synaptosomes treated with blackberry wine (6.81 U/mg), whereas blueberry wine induced the highest catalase and glutathione peroxidase activities (0.058 U/mg and 0.017 U/mg, respectively). Black chokeberry proved to be the best in lipid peroxidation protection with the lowest MDA value (1.42 nmol/mg). Finally, principal component analysis and hierarchical cluster analysis additionally highlighted a higher antioxidant capacity of wines produced from dark-skinned fruits (i.e., blackberry, black chokeberry, and blueberry). The results suggest protective effects of the fruit wines against oxidative damage, and, accordingly, their promising application as functional food.",
journal = "Agronomy",
title = "Protective effects of fruit wines against hydrogen peroxide—induced oxidative stress in rat synaptosomes",
volume = "11",
number = "7",
pages = "1414",
doi = "10.3390/agronomy11071414"
}

Čakar, U., Čolović, M. B., Milenković, D., Medić, B., Krstić, D. Z., Petrović, A.,& Ðoređvić, B.. (2021). Protective effects of fruit wines against hydrogen peroxide—induced oxidative stress in rat synaptosomes. in Agronomy, 11(7), 1414.
https://doi.org/10.3390/agronomy11071414

Čakar U, Čolović MB, Milenković D, Medić B, Krstić DZ, Petrović A, Ðoređvić B. Protective effects of fruit wines against hydrogen peroxide—induced oxidative stress in rat synaptosomes. in Agronomy. 2021;11(7):1414.
doi:10.3390/agronomy11071414 .

Čakar, Uroš, Čolović, Mirjana B., Milenković, Danijela, Medić, Branislava, Krstić, Danijela Z., Petrović, Aleksandar, Ðoređvić, Brižita, "Protective effects of fruit wines against hydrogen peroxide—induced oxidative stress in rat synaptosomes" in Agronomy, 11, no. 7 (2021):1414,
https://doi.org/10.3390/agronomy11071414 . .