TY  - JOUR
AU  - Filipović Tričković, Jelena G.
AU  - Momčilović, Miloš
AU  - Joksić, Gordana
AU  - Živković, Sanja
AU  - Ilić, Bojana
AU  - Ognjanović, Miloš
AU  - Novaković, Mirjana
AU  - Valenta-Šobot, Ana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11376
AB  - Silver nanoparticles (AgNPs) have been recognized for widespread biological applications due to their antimicrobial and anti-inflammatory effect, especially in dentistry and for wound healing. Many features determine their beneficial or toxic potential, such as their synthesis type, size, morphology, coating, and concentration. Most synthesis types rely on the use of synthetic chemicals, which contributes to their toxicity. We present an environmentally friendly method for “green” synthesis of AgNPs from the silver target by pulsed laser ablation in liquid (PLAL) using citrate as the stabilizing agent. Since AgNPs already have many dental applications, we examined their antibacterial effect against supragingival biofilm-forming bacteria and bacterial strains known to cause resistant dental infections. Their impact on human fibroblast cells’ cytotoxicity, proliferation (measured by XTT and Ki-67 immunofluorescence), pro/antioxidant balance, and lipid peroxidation (measured by PAB and LPP) was evaluated. AgNPs1 (21 nm) and AgNPs2 (15 nm) spherical nanoparticles with good overall stability were obtained. The highest tested dose of smaller nanoparticles (AgNPs2) displays not only an effective antibacterial effect against the tested oral bacteria strains but also a pro-oxidant and cytotoxic effect on fibroblast cells. Lower doses do not affect bacterial survival but increase the cell proliferation and metabolic activity and show an antioxidative effect, suggesting that different concentrations display a substantially opposite effect. Compared to larger AgNPs1, smaller AgNPs2 possess more potent biological effects, indicating that size plays a pivotal role in their activity. Such opposite outcomes could be important for their medical application, and high concentrations could be used for the inhibition of dental biofilm formation and resistant dental infections as well as proliferative conditions, while low doses could be beneficial in the treatment of atrophic and inflammatory disorders. Finally, we showed that silver-targeted PLAL, using citrate as a stabilizing agent, produces biologically potent nanoparticles that could have many applications depending on their size and concentration.
T2  - Nanomaterials and Nanotechnology
T1  - Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects
VL  - 2023
SP  - e9854356
DO  - 10.1155/2023/9854356
ER  - 

@article{
author = "Filipović Tričković, Jelena G. and Momčilović, Miloš and Joksić, Gordana and Živković, Sanja and Ilić, Bojana and Ognjanović, Miloš and Novaković, Mirjana and Valenta-Šobot, Ana",
year = "2023",
abstract = "Silver nanoparticles (AgNPs) have been recognized for widespread biological applications due to their antimicrobial and anti-inflammatory effect, especially in dentistry and for wound healing. Many features determine their beneficial or toxic potential, such as their synthesis type, size, morphology, coating, and concentration. Most synthesis types rely on the use of synthetic chemicals, which contributes to their toxicity. We present an environmentally friendly method for “green” synthesis of AgNPs from the silver target by pulsed laser ablation in liquid (PLAL) using citrate as the stabilizing agent. Since AgNPs already have many dental applications, we examined their antibacterial effect against supragingival biofilm-forming bacteria and bacterial strains known to cause resistant dental infections. Their impact on human fibroblast cells’ cytotoxicity, proliferation (measured by XTT and Ki-67 immunofluorescence), pro/antioxidant balance, and lipid peroxidation (measured by PAB and LPP) was evaluated. AgNPs1 (21 nm) and AgNPs2 (15 nm) spherical nanoparticles with good overall stability were obtained. The highest tested dose of smaller nanoparticles (AgNPs2) displays not only an effective antibacterial effect against the tested oral bacteria strains but also a pro-oxidant and cytotoxic effect on fibroblast cells. Lower doses do not affect bacterial survival but increase the cell proliferation and metabolic activity and show an antioxidative effect, suggesting that different concentrations display a substantially opposite effect. Compared to larger AgNPs1, smaller AgNPs2 possess more potent biological effects, indicating that size plays a pivotal role in their activity. Such opposite outcomes could be important for their medical application, and high concentrations could be used for the inhibition of dental biofilm formation and resistant dental infections as well as proliferative conditions, while low doses could be beneficial in the treatment of atrophic and inflammatory disorders. Finally, we showed that silver-targeted PLAL, using citrate as a stabilizing agent, produces biologically potent nanoparticles that could have many applications depending on their size and concentration.",
journal = "Nanomaterials and Nanotechnology",
title = "Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects",
volume = "2023",
pages = "e9854356",
doi = "10.1155/2023/9854356"
}

Filipović Tričković, J. G., Momčilović, M., Joksić, G., Živković, S., Ilić, B., Ognjanović, M., Novaković, M.,& Valenta-Šobot, A.. (2023). Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects. in Nanomaterials and Nanotechnology, 2023, e9854356.
https://doi.org/10.1155/2023/9854356

Filipović Tričković JG, Momčilović M, Joksić G, Živković S, Ilić B, Ognjanović M, Novaković M, Valenta-Šobot A. Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects. in Nanomaterials and Nanotechnology. 2023;2023:e9854356.
doi:10.1155/2023/9854356 .

Filipović Tričković, Jelena G., Momčilović, Miloš, Joksić, Gordana, Živković, Sanja, Ilić, Bojana, Ognjanović, Miloš, Novaković, Mirjana, Valenta-Šobot, Ana, "Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects" in Nanomaterials and Nanotechnology, 2023 (2023):e9854356,
https://doi.org/10.1155/2023/9854356 . .

TY  - CONF
AU  - Kokunešoski, Maja
AU  - Šaponjić, Aleksandra
AU  - Baščarević, Zvezdana
AU  - Jovanović, Ivan
AU  - Filipović Tričković, Jelena G.
AU  - Valenta-Šobot, Ana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11280
AB  - Material SBA-15 with all of its particles in the form of regular spheres was synthesised by template method using
Pluronic P123 (non-ionic triblock copolymer) as a surfactant and tetraethoxysilane (TEOS, 98%) as a source of silica.
The synthesis of spherical SBA-15 was carried out in acidic conditions according to method proposed by Zhao et al.
Instead of expensive commercial cetyltrimethylammonium bromide as a co-surfactant, a spent HCl solution obtained
after the chemical treatment of clay in an aqueous solution of HCl was used. The mixture was aged at a higher
temperature. After that, the spherical SBA-15 was made by drying at room temperature after washing with distilled
water and then calcining in flowing air to decompose the triblock copolymer. The continual presence of spheres
of diameters around 2 μm in the entire SBA-15 was confirmed by scanning electron microscopy. The Energy-dispersive
X-ray spectroscopy confirmed that the spheres consisted only of SiO2 in composition. In addition, X-ray diffraction and
Fourier-transform infrared spectroscopy methods were used to characterise SBA-15 material. To assess the cytotoxic
effects of newly synthesised SBA-15 material, HEK 293 cells were treated with particles and extracts obtained from
particles incubated 24 hours at 37 °C in serum-free DMEM medium and DMEM with 10% serum. Cells were treated
with concentrations of 100, 250, and 500 µg/mL for 72 hours. All treatments reduced cell viability in a concentrationdependent manner (p<0.001). Cytotoxicity was more pronounced in particle treatment, indicating that mechanical
damage was induced in cells, whereas the extraction process influences the level of toxicity since the sera-free medium
had the mildest effect. To acquire safe particles for human use, additional synthesis optimisation is required.
PB  - Association of Metallurgical Engineers of Serbia (AMES)
C3  - MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina
T1  - Preliminary cytotoxicity testing of newly synthesised SBA-15 material
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11280
ER  - 

@conference{
author = "Kokunešoski, Maja and Šaponjić, Aleksandra and Baščarević, Zvezdana and Jovanović, Ivan and Filipović Tričković, Jelena G. and Valenta-Šobot, Ana",
year = "2023",
abstract = "Material SBA-15 with all of its particles in the form of regular spheres was synthesised by template method using
Pluronic P123 (non-ionic triblock copolymer) as a surfactant and tetraethoxysilane (TEOS, 98%) as a source of silica.
The synthesis of spherical SBA-15 was carried out in acidic conditions according to method proposed by Zhao et al.
Instead of expensive commercial cetyltrimethylammonium bromide as a co-surfactant, a spent HCl solution obtained
after the chemical treatment of clay in an aqueous solution of HCl was used. The mixture was aged at a higher
temperature. After that, the spherical SBA-15 was made by drying at room temperature after washing with distilled
water and then calcining in flowing air to decompose the triblock copolymer. The continual presence of spheres
of diameters around 2 μm in the entire SBA-15 was confirmed by scanning electron microscopy. The Energy-dispersive
X-ray spectroscopy confirmed that the spheres consisted only of SiO2 in composition. In addition, X-ray diffraction and
Fourier-transform infrared spectroscopy methods were used to characterise SBA-15 material. To assess the cytotoxic
effects of newly synthesised SBA-15 material, HEK 293 cells were treated with particles and extracts obtained from
particles incubated 24 hours at 37 °C in serum-free DMEM medium and DMEM with 10% serum. Cells were treated
with concentrations of 100, 250, and 500 µg/mL for 72 hours. All treatments reduced cell viability in a concentrationdependent manner (p<0.001). Cytotoxicity was more pronounced in particle treatment, indicating that mechanical
damage was induced in cells, whereas the extraction process influences the level of toxicity since the sera-free medium
had the mildest effect. To acquire safe particles for human use, additional synthesis optimisation is required.",
publisher = "Association of Metallurgical Engineers of Serbia (AMES)",
journal = "MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina",
title = "Preliminary cytotoxicity testing of newly synthesised SBA-15 material",
pages = "60",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11280"
}

Kokunešoski, M., Šaponjić, A., Baščarević, Z., Jovanović, I., Filipović Tričković, J. G.,& Valenta-Šobot, A.. (2023). Preliminary cytotoxicity testing of newly synthesised SBA-15 material. in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina
Association of Metallurgical Engineers of Serbia (AMES)., 60.
https://hdl.handle.net/21.15107/rcub_vinar_11280

Kokunešoski M, Šaponjić A, Baščarević Z, Jovanović I, Filipović Tričković JG, Valenta-Šobot A. Preliminary cytotoxicity testing of newly synthesised SBA-15 material. in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina. 2023;:60.
https://hdl.handle.net/21.15107/rcub_vinar_11280 .

Kokunešoski, Maja, Šaponjić, Aleksandra, Baščarević, Zvezdana, Jovanović, Ivan, Filipović Tričković, Jelena G., Valenta-Šobot, Ana, "Preliminary cytotoxicity testing of newly synthesised SBA-15 material" in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina (2023):60,
https://hdl.handle.net/21.15107/rcub_vinar_11280 .

TY  - CONF
AU  - Filipović Tričković, Jelena G.
AU  - Kokunešoski, Maja
AU  - Momić, Tatjana
AU  - Jovanović, Ivan G.
AU  - Anastasov, Marina
AU  - Stojanović, Dragana
AU  - Valenta Šobot, Ana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11409
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book
T1  - Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures
T1  - Procena toksičnosti mezoporoznih nanočestica silicijum dioksida pri različitim postupcima ekstrakcije
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11409
ER  - 

@conference{
author = "Filipović Tričković, Jelena G. and Kokunešoski, Maja and Momić, Tatjana and Jovanović, Ivan G. and Anastasov, Marina and Stojanović, Dragana and Valenta Šobot, Ana",
year = "2023",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book",
title = "Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures, Procena toksičnosti mezoporoznih nanočestica silicijum dioksida pri različitim postupcima ekstrakcije",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11409"
}

Filipović Tričković, J. G., Kokunešoski, M., Momić, T., Jovanović, I. G., Anastasov, M., Stojanović, D.,& Valenta Šobot, A.. (2023). Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book.
https://hdl.handle.net/21.15107/rcub_vinar_11409

Filipović Tričković JG, Kokunešoski M, Momić T, Jovanović IG, Anastasov M, Stojanović D, Valenta Šobot A. Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_11409 .

Filipović Tričković, Jelena G., Kokunešoski, Maja, Momić, Tatjana, Jovanović, Ivan G., Anastasov, Marina, Stojanović, Dragana, Valenta Šobot, Ana, "Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book (2023),
https://hdl.handle.net/21.15107/rcub_vinar_11409 .

TY  - CONF
AU  - Popović, I.
AU  - Valenta Šobot, Ana
AU  - Filipović Tričković, Jelena
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Ralić, V.
AU  - Abu el Rub, Anamarija
AU  - Algarra, M.
AU  - Petković, M.
AU  - Stepić, Milutin
AU  - Nešić, M.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11808
AB  - In recent years researchers have developed new strategies to enhance the effectiveness of wound healing by combining nanoparticles and infra red (IR) light. For example, some studies have shown that nanoparticles can be used to enhance the absorption of near-infrared laser (NIR) light by tissues, leading to increased healing rates [1]. The influence of NIR light on proliferation, collagen production, and wound healing was tested on: keratocytes (HaCaT) and fibroblasts (MRC-5) cells that are used as model systems of human skin equivalents that comprise an epidermal and a dermal compartment of skin. Also, these cells were treated with carbon quantum dots/silver-based metal-organic framework composites (Ag-MoFs-NCDs and Ag-MoFs-SCDs), which previously showed high antibacterial activity [2], without and with laser light. Firstly, we have found the most convenient and effective CW laser intensity (16 mW/cm2) and illumination time (3 minutes), which is not too high and short enough to influence human cells' proliferation and metabolism positively. Additional chemical treatment with Ag-MoFs-NCDs and Ag-MoFs-SCDs results in a further increase in human cell viability. Our measurements showed that the proliferation index in laser-illuminated cells and cells treated with Ag-MoFs-SCDs was at the level of the untreated control. Furthermore, Ag-MoFs-SCDs treatment and laser illumination induced a mild, insignificant increase in cellular proliferation. On the other hand, Ag-MoFs-NCDs treatment led to a more pronounced, albeit not significant increase, in cellular proliferation, while Ag-MoFs-NCDs treatment combined with laser illumination significantly increased proliferation. Also, we have detected a mild change in collagen level estimated by hydroxyproline assay, which may indicate a positive outcome of combined laser illumination and treatment, taking into account that after 48 hours, a change in cell's response to the treatment could be noticed. Finally, based on migration assay, we observe a complete wound closure after 48 hours in fibroblast cells treated with Ag-MoFs-NCDs and near-infrared laser light, Fig. 1.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
T1  - Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing
SP  - 78
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11808
ER  - 

@conference{
author = "Popović, I. and Valenta Šobot, Ana and Filipović Tričković, Jelena and Korićanac, Lela and Žakula, Jelena and Ralić, V. and Abu el Rub, Anamarija and Algarra, M. and Petković, M. and Stepić, Milutin and Nešić, M.",
year = "2023",
abstract = "In recent years researchers have developed new strategies to enhance the effectiveness of wound healing by combining nanoparticles and infra red (IR) light. For example, some studies have shown that nanoparticles can be used to enhance the absorption of near-infrared laser (NIR) light by tissues, leading to increased healing rates [1]. The influence of NIR light on proliferation, collagen production, and wound healing was tested on: keratocytes (HaCaT) and fibroblasts (MRC-5) cells that are used as model systems of human skin equivalents that comprise an epidermal and a dermal compartment of skin. Also, these cells were treated with carbon quantum dots/silver-based metal-organic framework composites (Ag-MoFs-NCDs and Ag-MoFs-SCDs), which previously showed high antibacterial activity [2], without and with laser light. Firstly, we have found the most convenient and effective CW laser intensity (16 mW/cm2) and illumination time (3 minutes), which is not too high and short enough to influence human cells' proliferation and metabolism positively. Additional chemical treatment with Ag-MoFs-NCDs and Ag-MoFs-SCDs results in a further increase in human cell viability. Our measurements showed that the proliferation index in laser-illuminated cells and cells treated with Ag-MoFs-SCDs was at the level of the untreated control. Furthermore, Ag-MoFs-SCDs treatment and laser illumination induced a mild, insignificant increase in cellular proliferation. On the other hand, Ag-MoFs-NCDs treatment led to a more pronounced, albeit not significant increase, in cellular proliferation, while Ag-MoFs-NCDs treatment combined with laser illumination significantly increased proliferation. Also, we have detected a mild change in collagen level estimated by hydroxyproline assay, which may indicate a positive outcome of combined laser illumination and treatment, taking into account that after 48 hours, a change in cell's response to the treatment could be noticed. Finally, based on migration assay, we observe a complete wound closure after 48 hours in fibroblast cells treated with Ag-MoFs-NCDs and near-infrared laser light, Fig. 1.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade",
title = "Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing",
pages = "78-78",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11808"
}

Popović, I., Valenta Šobot, A., Filipović Tričković, J., Korićanac, L., Žakula, J., Ralić, V., Abu el Rub, A., Algarra, M., Petković, M., Stepić, M.,& Nešić, M.. (2023). Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
Belgrade : Vinča Institute of Nuclear Sciences., 78-78.
https://hdl.handle.net/21.15107/rcub_vinar_11808

Popović I, Valenta Šobot A, Filipović Tričković J, Korićanac L, Žakula J, Ralić V, Abu el Rub A, Algarra M, Petković M, Stepić M, Nešić M. Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade. 2023;:78-78.
https://hdl.handle.net/21.15107/rcub_vinar_11808 .

Popović, I., Valenta Šobot, Ana, Filipović Tričković, Jelena, Korićanac, Lela, Žakula, Jelena, Ralić, V., Abu el Rub, Anamarija, Algarra, M., Petković, M., Stepić, Milutin, Nešić, M., "Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing" in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade (2023):78-78,
https://hdl.handle.net/21.15107/rcub_vinar_11808 .

TY  - CONF
AU  - Nešić, M. D.
AU  - Filipović Tričković, Jelena
AU  - Valenta Šobot, Ana
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Popović, I.
AU  - Soto, J.
AU  - Algarra, M.
AU  - Ralić, V.
AU  - Abu el Rub, Anamarija
AU  - Matijević, M.
AU  - Stepić, Milutin
AU  - Petković, M.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11809
AB  - Carbon Dots (CDs) are biocompatible, fluorescent, water-soluble, and stable nanoparticles with a high potential to be used for vast biomedical applications [1,2]. We explore the application of CDs produced from natural sources, black carrots, as anti-cancer and imaging agents. These nanoparticles suppress cell growth of three different cancer cell lines, cervical (HeLa), pancreatic (PANC-1), and melanoma (A375) cell lines in vitro. However, the cytotoxic effect against A375 cells stands out, with only 20% of viable cells left after treatment (Fig.1(a)), antimetastatic potential, and a selectivity index higher than two, which indicates that the efficacy against melanoma cells is significantly greater than the toxicity against non-malignant cells (MRC-5). Furthermore, after the cellular uptake, green fluorescence was visible in the cytosol of A375 cells (Fig. 1 (b)). On the other hand, the DAPI stain for DNA was visible as a blue light in the cell nucleus. Moreover, cells with a higher intensity of green fluorescence in the nucleus, Fig. 1 (c) indicated with arrows, were the cells with condensed chromatin in the mitotic phase of the cell cycle (Fig. 1 (d) and (e)), which indicates that CDs interact with chromatin and that they could be used as a marker of cells mitosis and proliferation. In summary, we have demonstrated the great anti-cancer potential of black carrot CDs, for image-guided anti-cancer therapy of melanoma that can be used to recognize cell proliferation.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
T1  - Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles
SP  - 79
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11809
ER  - 

@conference{
author = "Nešić, M. D. and Filipović Tričković, Jelena and Valenta Šobot, Ana and Žakula, Jelena and Korićanac, Lela and Popović, I. and Soto, J. and Algarra, M. and Ralić, V. and Abu el Rub, Anamarija and Matijević, M. and Stepić, Milutin and Petković, M.",
year = "2023",
abstract = "Carbon Dots (CDs) are biocompatible, fluorescent, water-soluble, and stable nanoparticles with a high potential to be used for vast biomedical applications [1,2]. We explore the application of CDs produced from natural sources, black carrots, as anti-cancer and imaging agents. These nanoparticles suppress cell growth of three different cancer cell lines, cervical (HeLa), pancreatic (PANC-1), and melanoma (A375) cell lines in vitro. However, the cytotoxic effect against A375 cells stands out, with only 20% of viable cells left after treatment (Fig.1(a)), antimetastatic potential, and a selectivity index higher than two, which indicates that the efficacy against melanoma cells is significantly greater than the toxicity against non-malignant cells (MRC-5). Furthermore, after the cellular uptake, green fluorescence was visible in the cytosol of A375 cells (Fig. 1 (b)). On the other hand, the DAPI stain for DNA was visible as a blue light in the cell nucleus. Moreover, cells with a higher intensity of green fluorescence in the nucleus, Fig. 1 (c) indicated with arrows, were the cells with condensed chromatin in the mitotic phase of the cell cycle (Fig. 1 (d) and (e)), which indicates that CDs interact with chromatin and that they could be used as a marker of cells mitosis and proliferation. In summary, we have demonstrated the great anti-cancer potential of black carrot CDs, for image-guided anti-cancer therapy of melanoma that can be used to recognize cell proliferation.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade",
title = "Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles",
pages = "79-79",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11809"
}

Nešić, M. D., Filipović Tričković, J., Valenta Šobot, A., Žakula, J., Korićanac, L., Popović, I., Soto, J., Algarra, M., Ralić, V., Abu el Rub, A., Matijević, M., Stepić, M.,& Petković, M.. (2023). Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
Belgrade : Vinča Institute of Nuclear Sciences., 79-79.
https://hdl.handle.net/21.15107/rcub_vinar_11809

Nešić MD, Filipović Tričković J, Valenta Šobot A, Žakula J, Korićanac L, Popović I, Soto J, Algarra M, Ralić V, Abu el Rub A, Matijević M, Stepić M, Petković M. Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade. 2023;:79-79.
https://hdl.handle.net/21.15107/rcub_vinar_11809 .

Nešić, M. D., Filipović Tričković, Jelena, Valenta Šobot, Ana, Žakula, Jelena, Korićanac, Lela, Popović, I., Soto, J., Algarra, M., Ralić, V., Abu el Rub, Anamarija, Matijević, M., Stepić, Milutin, Petković, M., "Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles" in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade (2023):79-79,
https://hdl.handle.net/21.15107/rcub_vinar_11809 .

TY  - CONF
AU  - Valenta Šobot, Ana
AU  - Drakulić, Dunja
AU  - Kokunešoski, Maja
AU  - Momić, Tatjana
AU  - Filipović Tričković, Jelena G.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11410
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book
T1  - Loganic acid induces apoptosis in human peripheral blood mononuclear cells
T1  - Loganska kiselina indukuje apoptozu u mononuklearnim ćelijama periferne krvi čoveka
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11410
ER  - 

@conference{
author = "Valenta Šobot, Ana and Drakulić, Dunja and Kokunešoski, Maja and Momić, Tatjana and Filipović Tričković, Jelena G.",
year = "2023",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book",
title = "Loganic acid induces apoptosis in human peripheral blood mononuclear cells, Loganska kiselina indukuje apoptozu u mononuklearnim ćelijama periferne krvi čoveka",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11410"
}

Valenta Šobot, A., Drakulić, D., Kokunešoski, M., Momić, T.,& Filipović Tričković, J. G.. (2023). Loganic acid induces apoptosis in human peripheral blood mononuclear cells. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book.
https://hdl.handle.net/21.15107/rcub_vinar_11410

Valenta Šobot A, Drakulić D, Kokunešoski M, Momić T, Filipović Tričković JG. Loganic acid induces apoptosis in human peripheral blood mononuclear cells. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_11410 .

Valenta Šobot, Ana, Drakulić, Dunja, Kokunešoski, Maja, Momić, Tatjana, Filipović Tričković, Jelena G., "Loganic acid induces apoptosis in human peripheral blood mononuclear cells" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book (2023),
https://hdl.handle.net/21.15107/rcub_vinar_11410 .

TY  - CONF
AU  - Valenta Šobot, Ana
AU  - Filipović Tričković, Jelena
AU  - Drakulić, Dunja
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Momić, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11582
AB  - Sweroside (Sw) is a secondary metabolite commonly found in plants belonging to the Gentianaceae family [1]. This iridoid compound is well-known for its anti-inflammatory [2], antidiabetic [3] and antitumor properties [4], which have been studied in pathological model systems. In transformed cell lines, Sw displays an antitumor effect by apoptosis activation [5]. Since healthy cells are also exposed to cancer therapy applied in vivo, our goal was to determine Sw’s cytotoxic concentration in primary human peripheral blood mononuclear cells (PBMCs) after 48 h of treatment with a range of concentration from 20 μM to 130 μM Sw in vitro, and to analyze whether the cytotoxic effect was due to activated apoptosis. According to the obtained results of the trypan blue dye exclusion test, 48 h of treatment with 50 μM Sw and higher concentrations led to a significant decrease in cell number. The DNA fragmentation assay indicated that following 50 μM Sw treatment, cells are dying in an apoptosis-like manner since the level of DNA fragments was 3.5 times higher than in the untreated control. The type of cell death was confirmed by immunoblot analysis of apoptosis- specific protein markers, which revealed the elevation of cleaved caspase-3 and PARP1 89 kDa fragments. Our findings showed that like in transformed cell lines, Sw in healthy cells can also activate apoptosis. A potential difference in sensitivity to Sw treatment between healthy and transformed cells could justify Sw treatment in anticancer therapy.
PB  - Belgrade : University of Belgrade, Faculty of Agriculture
C3  - 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
T1  - Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells
SP  - 47
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11582
ER  - 

@conference{
author = "Valenta Šobot, Ana and Filipović Tričković, Jelena and Drakulić, Dunja and Leskovac, Andreja and Petrović, Sandra and Momić, Tatjana",
year = "2022",
abstract = "Sweroside (Sw) is a secondary metabolite commonly found in plants belonging to the Gentianaceae family [1]. This iridoid compound is well-known for its anti-inflammatory [2], antidiabetic [3] and antitumor properties [4], which have been studied in pathological model systems. In transformed cell lines, Sw displays an antitumor effect by apoptosis activation [5]. Since healthy cells are also exposed to cancer therapy applied in vivo, our goal was to determine Sw’s cytotoxic concentration in primary human peripheral blood mononuclear cells (PBMCs) after 48 h of treatment with a range of concentration from 20 μM to 130 μM Sw in vitro, and to analyze whether the cytotoxic effect was due to activated apoptosis. According to the obtained results of the trypan blue dye exclusion test, 48 h of treatment with 50 μM Sw and higher concentrations led to a significant decrease in cell number. The DNA fragmentation assay indicated that following 50 μM Sw treatment, cells are dying in an apoptosis-like manner since the level of DNA fragments was 3.5 times higher than in the untreated control. The type of cell death was confirmed by immunoblot analysis of apoptosis- specific protein markers, which revealed the elevation of cleaved caspase-3 and PARP1 89 kDa fragments. Our findings showed that like in transformed cell lines, Sw in healthy cells can also activate apoptosis. A potential difference in sensitivity to Sw treatment between healthy and transformed cells could justify Sw treatment in anticancer therapy.",
publisher = "Belgrade : University of Belgrade, Faculty of Agriculture",
journal = "1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts",
title = "Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells",
pages = "47-47",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11582"
}

Valenta Šobot, A., Filipović Tričković, J., Drakulić, D., Leskovac, A., Petrović, S.,& Momić, T.. (2022). Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
Belgrade : University of Belgrade, Faculty of Agriculture., 47-47.
https://hdl.handle.net/21.15107/rcub_vinar_11582

Valenta Šobot A, Filipović Tričković J, Drakulić D, Leskovac A, Petrović S, Momić T. Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts. 2022;:47-47.
https://hdl.handle.net/21.15107/rcub_vinar_11582 .

Valenta Šobot, Ana, Filipović Tričković, Jelena, Drakulić, Dunja, Leskovac, Andreja, Petrović, Sandra, Momić, Tatjana, "Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells" in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts (2022):47-47,
https://hdl.handle.net/21.15107/rcub_vinar_11582 .

TY  - CONF
AU  - Valenta Šobot, Ana
AU  - Filipović Tričković, Jelena
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Momić, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11583
AB  - Quercetin (Q) is one of the most common and well researched antioxidant flavonoids, which usually occurs in plant-based foods, and medicinal plants. It was shown that quercetin exerts many beneficial effects on human health, including prevention of cancer and heart diseases. Quercetin was found to be toxic toward various types of cancer cell, still, at higher concentrations it was also shown toxic toward normal human cells [1,2]. One of the approaches to overcome this shortcoming offers nanotechnology which enables the novel perspective of phytochemical usage in contemporary medicine [3]. The strategy of binding quercetin to the gold nanoparticles during their synthesis was used, which resulted in quercetin capped gold nanoparticles (NPQ) [4]. Trypan blue exclusion test [5] was used to evaluate peripheral blood mononuclear cells (PBMC) viability after their exposure to either NPQ or free Q during 24, 48 and 72 h, at 37 °C, in the range of quercetin concentrations from 5 to 50 μg/mL. A significant reduction in the cell count was observed in PBMC cultures treated with 10, 20, and 50 μg/mL of free Q, for all exposure times. The treatments of increasing concentrations and exposure times lowered the cells viability, resulting in 63% of the viable cells, following 72 h of the treatment with 50 μg/mL of free Q. Although NPQ treatments affected the cells viability in a concentration- and time-dependent manner the treatment with 50 μg/mL of NPQ for 72 h, had a milder effect on PBMC cultures than free Q, resulting in 81% of the viable cells (Figure 1). According to the obtained results, NPQ were shown less toxic toward PBMC than free Q.
PB  - Belgrade : University of Belgrade, Faculty of Agriculture
C3  - 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
T1  - Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells
SP  - 64
EP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11583
ER  - 

@conference{
author = "Valenta Šobot, Ana and Filipović Tričković, Jelena and Leskovac, Andreja and Petrović, Sandra and Momić, Tatjana",
year = "2022",
abstract = "Quercetin (Q) is one of the most common and well researched antioxidant flavonoids, which usually occurs in plant-based foods, and medicinal plants. It was shown that quercetin exerts many beneficial effects on human health, including prevention of cancer and heart diseases. Quercetin was found to be toxic toward various types of cancer cell, still, at higher concentrations it was also shown toxic toward normal human cells [1,2]. One of the approaches to overcome this shortcoming offers nanotechnology which enables the novel perspective of phytochemical usage in contemporary medicine [3]. The strategy of binding quercetin to the gold nanoparticles during their synthesis was used, which resulted in quercetin capped gold nanoparticles (NPQ) [4]. Trypan blue exclusion test [5] was used to evaluate peripheral blood mononuclear cells (PBMC) viability after their exposure to either NPQ or free Q during 24, 48 and 72 h, at 37 °C, in the range of quercetin concentrations from 5 to 50 μg/mL. A significant reduction in the cell count was observed in PBMC cultures treated with 10, 20, and 50 μg/mL of free Q, for all exposure times. The treatments of increasing concentrations and exposure times lowered the cells viability, resulting in 63% of the viable cells, following 72 h of the treatment with 50 μg/mL of free Q. Although NPQ treatments affected the cells viability in a concentration- and time-dependent manner the treatment with 50 μg/mL of NPQ for 72 h, had a milder effect on PBMC cultures than free Q, resulting in 81% of the viable cells (Figure 1). According to the obtained results, NPQ were shown less toxic toward PBMC than free Q.",
publisher = "Belgrade : University of Belgrade, Faculty of Agriculture",
journal = "1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts",
title = "Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells",
pages = "64-64",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11583"
}

Valenta Šobot, A., Filipović Tričković, J., Leskovac, A., Petrović, S.,& Momić, T.. (2022). Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
Belgrade : University of Belgrade, Faculty of Agriculture., 64-64.
https://hdl.handle.net/21.15107/rcub_vinar_11583

Valenta Šobot A, Filipović Tričković J, Leskovac A, Petrović S, Momić T. Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts. 2022;:64-64.
https://hdl.handle.net/21.15107/rcub_vinar_11583 .

Valenta Šobot, Ana, Filipović Tričković, Jelena, Leskovac, Andreja, Petrović, Sandra, Momić, Tatjana, "Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells" in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts (2022):64-64,
https://hdl.handle.net/21.15107/rcub_vinar_11583 .

TY  - CONF
AU  - Tasić, T.
AU  - Milanković, Vedran
AU  - Kokunešoski, Maja
AU  - Šaponjić, Aleksandra
AU  - Valenta-Šobot, Ana
AU  - Grce, Ana
AU  - Lazarević-Pašti, Tamara
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11285
AB  - Organophosphate pesticides are primarily used in agriculture despite their high
neurotoxicity to humans and the environment. Malathion is used in agriculture as a means
of controlling the development of pests, but also in the protection of public health, to
control mosquitoes and lice. However, this organophosphate compound is hazardous to
human health. We used wood-waste-derived activated porous carbon material as an
adsorbent for malathion removal from water. The kinetics of the malathion adsorption
process on investigated carbon material at different temperatures was studied. The results
showed that the experimental data are a good fit with the pseudo-second-order model.
The calculated rate constants were 0.009 and 0.007 g mg−1 min−1
at 20 and 40 °C,
respectively. The equilibrium was reached after 60 minutes at both investigated
temperatures. It was shown that 1 g of the studied material could adsorb up to 186.22 mg
of malathion at 20 °C.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts
T1  - Wood-Waste-Derived Activated Porous Carbon Material for Pesticide Removal From Water
SP  - 135
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11285
ER  - 

@conference{
author = "Tasić, T. and Milanković, Vedran and Kokunešoski, Maja and Šaponjić, Aleksandra and Valenta-Šobot, Ana and Grce, Ana and Lazarević-Pašti, Tamara",
year = "2022",
abstract = "Organophosphate pesticides are primarily used in agriculture despite their high
neurotoxicity to humans and the environment. Malathion is used in agriculture as a means
of controlling the development of pests, but also in the protection of public health, to
control mosquitoes and lice. However, this organophosphate compound is hazardous to
human health. We used wood-waste-derived activated porous carbon material as an
adsorbent for malathion removal from water. The kinetics of the malathion adsorption
process on investigated carbon material at different temperatures was studied. The results
showed that the experimental data are a good fit with the pseudo-second-order model.
The calculated rate constants were 0.009 and 0.007 g mg−1 min−1
at 20 and 40 °C,
respectively. The equilibrium was reached after 60 minutes at both investigated
temperatures. It was shown that 1 g of the studied material could adsorb up to 186.22 mg
of malathion at 20 °C.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts",
title = "Wood-Waste-Derived Activated Porous Carbon Material for Pesticide Removal From Water",
pages = "135-135",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11285"
}

Tasić, T., Milanković, V., Kokunešoski, M., Šaponjić, A., Valenta-Šobot, A., Grce, A.,& Lazarević-Pašti, T.. (2022). Wood-Waste-Derived Activated Porous Carbon Material for Pesticide Removal From Water. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts
Society of Physical Chemists of Serbia., 135-135.
https://hdl.handle.net/21.15107/rcub_vinar_11285

Tasić T, Milanković V, Kokunešoski M, Šaponjić A, Valenta-Šobot A, Grce A, Lazarević-Pašti T. Wood-Waste-Derived Activated Porous Carbon Material for Pesticide Removal From Water. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts. 2022;:135-135.
https://hdl.handle.net/21.15107/rcub_vinar_11285 .

Tasić, T., Milanković, Vedran, Kokunešoski, Maja, Šaponjić, Aleksandra, Valenta-Šobot, Ana, Grce, Ana, Lazarević-Pašti, Tamara, "Wood-Waste-Derived Activated Porous Carbon Material for Pesticide Removal From Water" in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts (2022):135-135,
https://hdl.handle.net/21.15107/rcub_vinar_11285 .

TY  - JOUR
AU  - Filipović Tričković, Jelena G.
AU  - Valenta-Šobot, Ana
AU  - Joksić, Ivana
AU  - Joksić, Gordana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10258
AB  - AbstractBiološki učinci ionizirajućega zračenja (IZ) pripisuju se oštećenjima DNA i indirektnim učincima kroz povećanu proizvodnju reaktivnih vrsta kisika. Iako se telomere rabe kao pokazatelji radioosjetljivosti, o njihovu ponašanju kao odgovoru na ionizirajuće zračenje u uvjetima profesionalne izloženosti i dalje se raspravlja. U ovom radu željeli smo istražiti duljinu i strukturu telomera u bolničkih radnika koji su profesionalno izloženi ionizirajućem zračenju te povezati te nalaze s oksidacijskim biomolekulama i kromosomskim aberacijama. Uzorci krvi izloženih ispitanika i zdravih kontrola uzeti su za analizu tijekom rutinskoga godišnjeg zdravstvenog pregleda. Osim kromosomskih aberacija, u uzorcima plazme izmjereni su i parametri oksidacijskoga stresa [prooksidacijska/antioksidacijska ravnoteža (PAB), lipidna peroksidacija i 8-okso-dG], a procjena duljine i strukture telomera provedena je metodom Q-FISH na metafaznim kromosomima. Analiza kromosomskih aberacija pokazala je da od 34 ispitanika njih 14 ima kromosomske aberacije (skupina 1), a 20 nije imalo aberacije (skupina 2). Nije bilo značajne razlike u spolu ili dobi ni u duljini telomera između skupina. Međutim, incidencija lomljivih telomera bila je značajno veća u objema skupinama ispitanika izloženih IZ-u u usporedbi s kontrolnim ispitanicima. Produkti peroksidacije lipida i 8-okso-dG također su bili značajno viši u objema skupinama. Učestalost lomljivih telomera u pozitivnoj je korelaciji (statistički značajna) s razinama 8-okso-dG u
T2  - Archives of Industrial Hygiene and Toxicology
T1  - Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation
VL  - 73
IS  - 1
SP  - 23
EP  - 30
DO  - 10.2478/aiht-2022-73-3609
ER  - 

@article{
author = "Filipović Tričković, Jelena G. and Valenta-Šobot, Ana and Joksić, Ivana and Joksić, Gordana",
year = "2022",
abstract = "AbstractBiološki učinci ionizirajućega zračenja (IZ) pripisuju se oštećenjima DNA i indirektnim učincima kroz povećanu proizvodnju reaktivnih vrsta kisika. Iako se telomere rabe kao pokazatelji radioosjetljivosti, o njihovu ponašanju kao odgovoru na ionizirajuće zračenje u uvjetima profesionalne izloženosti i dalje se raspravlja. U ovom radu željeli smo istražiti duljinu i strukturu telomera u bolničkih radnika koji su profesionalno izloženi ionizirajućem zračenju te povezati te nalaze s oksidacijskim biomolekulama i kromosomskim aberacijama. Uzorci krvi izloženih ispitanika i zdravih kontrola uzeti su za analizu tijekom rutinskoga godišnjeg zdravstvenog pregleda. Osim kromosomskih aberacija, u uzorcima plazme izmjereni su i parametri oksidacijskoga stresa [prooksidacijska/antioksidacijska ravnoteža (PAB), lipidna peroksidacija i 8-okso-dG], a procjena duljine i strukture telomera provedena je metodom Q-FISH na metafaznim kromosomima. Analiza kromosomskih aberacija pokazala je da od 34 ispitanika njih 14 ima kromosomske aberacije (skupina 1), a 20 nije imalo aberacije (skupina 2). Nije bilo značajne razlike u spolu ili dobi ni u duljini telomera između skupina. Međutim, incidencija lomljivih telomera bila je značajno veća u objema skupinama ispitanika izloženih IZ-u u usporedbi s kontrolnim ispitanicima. Produkti peroksidacije lipida i 8-okso-dG također su bili značajno viši u objema skupinama. Učestalost lomljivih telomera u pozitivnoj je korelaciji (statistički značajna) s razinama 8-okso-dG u",
journal = "Archives of Industrial Hygiene and Toxicology",
title = "Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation",
volume = "73",
number = "1",
pages = "23-30",
doi = "10.2478/aiht-2022-73-3609"
}

Filipović Tričković, J. G., Valenta-Šobot, A., Joksić, I.,& Joksić, G.. (2022). Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation. in Archives of Industrial Hygiene and Toxicology, 73(1), 23-30.
https://doi.org/10.2478/aiht-2022-73-3609

Filipović Tričković JG, Valenta-Šobot A, Joksić I, Joksić G. Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation. in Archives of Industrial Hygiene and Toxicology. 2022;73(1):23-30.
doi:10.2478/aiht-2022-73-3609 .

Filipović Tričković, Jelena G., Valenta-Šobot, Ana, Joksić, Ivana, Joksić, Gordana, "Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation" in Archives of Industrial Hygiene and Toxicology, 73, no. 1 (2022):23-30,
https://doi.org/10.2478/aiht-2022-73-3609 . .

TY  - JOUR
AU  - Matijević, Milica
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Radoičić, Marija B.
AU  - Liang, Xinyue
AU  - Mi, Lan
AU  - Filipović Tričković, Jelena G.
AU  - Valenta-Šobot, Ana
AU  - Stanković, Maja N.
AU  - Nakarada, Đura
AU  - Mojović, Miloš
AU  - Petković, Marijana
AU  - Stepić, Milutin
AU  - Nešić, Maja D.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9908
AB  - In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy.
T2  - Photochemical and Photobiological Sciences
T1  - Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles
VL  - 20
IS  - 8
SP  - 1087
EP  - 1098
DO  - 10.1007/s43630-021-00082-2
ER  - 

@article{
author = "Matijević, Milica and Žakula, Jelena and Korićanac, Lela and Radoičić, Marija B. and Liang, Xinyue and Mi, Lan and Filipović Tričković, Jelena G. and Valenta-Šobot, Ana and Stanković, Maja N. and Nakarada, Đura and Mojović, Miloš and Petković, Marijana and Stepić, Milutin and Nešić, Maja D.",
year = "2021",
abstract = "In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy.",
journal = "Photochemical and Photobiological Sciences",
title = "Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles",
volume = "20",
number = "8",
pages = "1087-1098",
doi = "10.1007/s43630-021-00082-2"
}

Matijević, M., Žakula, J., Korićanac, L., Radoičić, M. B., Liang, X., Mi, L., Filipović Tričković, J. G., Valenta-Šobot, A., Stanković, M. N., Nakarada, Đ., Mojović, M., Petković, M., Stepić, M.,& Nešić, M. D.. (2021). Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles. in Photochemical and Photobiological Sciences, 20(8), 1087-1098.
https://doi.org/10.1007/s43630-021-00082-2

Matijević M, Žakula J, Korićanac L, Radoičić MB, Liang X, Mi L, Filipović Tričković JG, Valenta-Šobot A, Stanković MN, Nakarada Đ, Mojović M, Petković M, Stepić M, Nešić MD. Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles. in Photochemical and Photobiological Sciences. 2021;20(8):1087-1098.
doi:10.1007/s43630-021-00082-2 .

Matijević, Milica, Žakula, Jelena, Korićanac, Lela, Radoičić, Marija B., Liang, Xinyue, Mi, Lan, Filipović Tričković, Jelena G., Valenta-Šobot, Ana, Stanković, Maja N., Nakarada, Đura, Mojović, Miloš, Petković, Marijana, Stepić, Milutin, Nešić, Maja D., "Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles" in Photochemical and Photobiological Sciences, 20, no. 8 (2021):1087-1098,
https://doi.org/10.1007/s43630-021-00082-2 . .

TY  - CONF
AU  - Valenta-Šobot, Ana
AU  - Filipović Tričković, Jelena G.
AU  - Šaponjić, Aleksandra
AU  - Ilić, Svetlana
AU  - Grce, Ana
AU  - Katnić, Đurica
AU  - Kokunešoski, Maja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11293
AB  - Mesoporous silica renders chemical and mechanical stability under biological conditions, but due to its physicochemical properties, could be potentially harmful to exposed cells. The aim of the current study was to estimate SBA-15 (concentration of 50, 100 and 250 µg/mL) impact on human peripheral blood mononuclear cells to induce cyto- and genotoxicity in vitro after 72 h treatment, as well as lipid peroxidation in serum samples ex vivo. SBA-15 mesoporous silica treatment impact on cell viability was performed by XTT assay, lipid peroxidation was estimated by determining malondialdehyde and 4-hydroxyalkenals levels and genotoxicity by micronucleus assay. SBA-15 treatments displayed genotoxic potential at the lowest concentration, while highest tested concentration led to decrease of cell viability and increase of lipid peroxidation. Chemical modification of SBA-15 material that could influence its physicochemical properties could be useful way to lower its toxicity.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry
T1  - Mesoporous silica decrease cell viability in vitro in dose dependent manner
VL  - II
SP  - 442
EP  - 444
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11293
ER  - 

@conference{
author = "Valenta-Šobot, Ana and Filipović Tričković, Jelena G. and Šaponjić, Aleksandra and Ilić, Svetlana and Grce, Ana and Katnić, Đurica and Kokunešoski, Maja",
year = "2021",
abstract = "Mesoporous silica renders chemical and mechanical stability under biological conditions, but due to its physicochemical properties, could be potentially harmful to exposed cells. The aim of the current study was to estimate SBA-15 (concentration of 50, 100 and 250 µg/mL) impact on human peripheral blood mononuclear cells to induce cyto- and genotoxicity in vitro after 72 h treatment, as well as lipid peroxidation in serum samples ex vivo. SBA-15 mesoporous silica treatment impact on cell viability was performed by XTT assay, lipid peroxidation was estimated by determining malondialdehyde and 4-hydroxyalkenals levels and genotoxicity by micronucleus assay. SBA-15 treatments displayed genotoxic potential at the lowest concentration, while highest tested concentration led to decrease of cell viability and increase of lipid peroxidation. Chemical modification of SBA-15 material that could influence its physicochemical properties could be useful way to lower its toxicity.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry",
title = "Mesoporous silica decrease cell viability in vitro in dose dependent manner",
volume = "II",
pages = "442-444",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11293"
}

Valenta-Šobot, A., Filipović Tričković, J. G., Šaponjić, A., Ilić, S., Grce, A., Katnić, Đ.,& Kokunešoski, M.. (2021). Mesoporous silica decrease cell viability in vitro in dose dependent manner. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., II, 442-444.
https://hdl.handle.net/21.15107/rcub_vinar_11293

Valenta-Šobot A, Filipović Tričković JG, Šaponjić A, Ilić S, Grce A, Katnić Đ, Kokunešoski M. Mesoporous silica decrease cell viability in vitro in dose dependent manner. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry. 2021;II:442-444.
https://hdl.handle.net/21.15107/rcub_vinar_11293 .

Valenta-Šobot, Ana, Filipović Tričković, Jelena G., Šaponjić, Aleksandra, Ilić, Svetlana, Grce, Ana, Katnić, Đurica, Kokunešoski, Maja, "Mesoporous silica decrease cell viability in vitro in dose dependent manner" in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry, II (2021):442-444,
https://hdl.handle.net/21.15107/rcub_vinar_11293 .

TY  - CONF
AU  - Kokunešoski, Maja
AU  - Baščarević, Zvezdana
AU  - Ilić, Svetlana
AU  - Valenta-Šobot, Ana
AU  - Grce, Ana
AU  - Pošarac-Marković, Milica
AU  - Šaponjić, Đorđe
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11290
AB  - The template method synthesis of both SBA-15 materials with elongated and spherical particles was performed using a surfactant Pluronic P123. The HCl (p.a.) was used to synthesize material with elongated particles connected in chain structures grouped into shapes resembling sheaves of wheat. In the synthesis of spherical SBA-15 with diameters ranging from 0.5 to 2 μm, a spent HCl solution which was obtained after chemical treatment of clay was used where the dominant presence of the spheres was confirmed by the SEM method. In addition to the methods mentioned above, XRD, EDS and FTIR methods characterize SBA-15 materials.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry
T1  - The influence of hydrochloric acid on the features of SBA-15 particles
VL  - II
SP  - 438
EP  - 441
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11290
ER  - 

@conference{
author = "Kokunešoski, Maja and Baščarević, Zvezdana and Ilić, Svetlana and Valenta-Šobot, Ana and Grce, Ana and Pošarac-Marković, Milica and Šaponjić, Đorđe",
year = "2021",
abstract = "The template method synthesis of both SBA-15 materials with elongated and spherical particles was performed using a surfactant Pluronic P123. The HCl (p.a.) was used to synthesize material with elongated particles connected in chain structures grouped into shapes resembling sheaves of wheat. In the synthesis of spherical SBA-15 with diameters ranging from 0.5 to 2 μm, a spent HCl solution which was obtained after chemical treatment of clay was used where the dominant presence of the spheres was confirmed by the SEM method. In addition to the methods mentioned above, XRD, EDS and FTIR methods characterize SBA-15 materials.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry",
title = "The influence of hydrochloric acid on the features of SBA-15 particles",
volume = "II",
pages = "438-441",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11290"
}

Kokunešoski, M., Baščarević, Z., Ilić, S., Valenta-Šobot, A., Grce, A., Pošarac-Marković, M.,& Šaponjić, Đ.. (2021). The influence of hydrochloric acid on the features of SBA-15 particles. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., II, 438-441.
https://hdl.handle.net/21.15107/rcub_vinar_11290

Kokunešoski M, Baščarević Z, Ilić S, Valenta-Šobot A, Grce A, Pošarac-Marković M, Šaponjić Đ. The influence of hydrochloric acid on the features of SBA-15 particles. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry. 2021;II:438-441.
https://hdl.handle.net/21.15107/rcub_vinar_11290 .

Kokunešoski, Maja, Baščarević, Zvezdana, Ilić, Svetlana, Valenta-Šobot, Ana, Grce, Ana, Pošarac-Marković, Milica, Šaponjić, Đorđe, "The influence of hydrochloric acid on the features of SBA-15 particles" in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry, II (2021):438-441,
https://hdl.handle.net/21.15107/rcub_vinar_11290 .

TY  - CONF
AU  - Valenta-Šobot, Ana
AU  - Filipović Tričković, Jelena G.
AU  - Šaponjić, Aleksandra
AU  - Grce, Ana
AU  - Kokunešoski, Maja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11310
PB  - Academy of Sciences and Arts of the Republic of Srpska
C3  - „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts
T1  - Prolonged exposure to mesoporous silica decrease cell viability in vitro
SP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11310
ER  - 

@conference{
author = "Valenta-Šobot, Ana and Filipović Tričković, Jelena G. and Šaponjić, Aleksandra and Grce, Ana and Kokunešoski, Maja",
year = "2021",
publisher = "Academy of Sciences and Arts of the Republic of Srpska",
journal = "„Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts",
title = "Prolonged exposure to mesoporous silica decrease cell viability in vitro",
pages = "53",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11310"
}

Valenta-Šobot, A., Filipović Tričković, J. G., Šaponjić, A., Grce, A.,& Kokunešoski, M.. (2021). Prolonged exposure to mesoporous silica decrease cell viability in vitro. in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts
Academy of Sciences and Arts of the Republic of Srpska., 53.
https://hdl.handle.net/21.15107/rcub_vinar_11310

Valenta-Šobot A, Filipović Tričković JG, Šaponjić A, Grce A, Kokunešoski M. Prolonged exposure to mesoporous silica decrease cell viability in vitro. in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts. 2021;:53.
https://hdl.handle.net/21.15107/rcub_vinar_11310 .

Valenta-Šobot, Ana, Filipović Tričković, Jelena G., Šaponjić, Aleksandra, Grce, Ana, Kokunešoski, Maja, "Prolonged exposure to mesoporous silica decrease cell viability in vitro" in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts (2021):53,
https://hdl.handle.net/21.15107/rcub_vinar_11310 .

TY  - CONF
AU  - Katnić, Đurica
AU  - Kokunešoski, Maja
AU  - Šaponjić, Aleksandra
AU  - Valenta-Šobot, Ana
AU  - Pošarac-Marković, Milica
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11308
PB  - Academy of Sciences and Arts of the Republic of Srpska
C3  - „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts
T1  - Influence of synthesis conditions on adsorption capacity of SBA-15/alginate adsorbent for removal ions of nickel(II) and lead(II) from aqueous solution
SP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11308
ER  - 

@conference{
author = "Katnić, Đurica and Kokunešoski, Maja and Šaponjić, Aleksandra and Valenta-Šobot, Ana and Pošarac-Marković, Milica",
year = "2021",
publisher = "Academy of Sciences and Arts of the Republic of Srpska",
journal = "„Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts",
title = "Influence of synthesis conditions on adsorption capacity of SBA-15/alginate adsorbent for removal ions of nickel(II) and lead(II) from aqueous solution",
pages = "49",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11308"
}

Katnić, Đ., Kokunešoski, M., Šaponjić, A., Valenta-Šobot, A.,& Pošarac-Marković, M.. (2021). Influence of synthesis conditions on adsorption capacity of SBA-15/alginate adsorbent for removal ions of nickel(II) and lead(II) from aqueous solution. in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts
Academy of Sciences and Arts of the Republic of Srpska., 49.
https://hdl.handle.net/21.15107/rcub_vinar_11308

Katnić Đ, Kokunešoski M, Šaponjić A, Valenta-Šobot A, Pošarac-Marković M. Influence of synthesis conditions on adsorption capacity of SBA-15/alginate adsorbent for removal ions of nickel(II) and lead(II) from aqueous solution. in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts. 2021;:49.
https://hdl.handle.net/21.15107/rcub_vinar_11308 .

Katnić, Đurica, Kokunešoski, Maja, Šaponjić, Aleksandra, Valenta-Šobot, Ana, Pošarac-Marković, Milica, "Influence of synthesis conditions on adsorption capacity of SBA-15/alginate adsorbent for removal ions of nickel(II) and lead(II) from aqueous solution" in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts (2021):49,
https://hdl.handle.net/21.15107/rcub_vinar_11308 .

TY  - CONF
AU  - Kokunešoski, Maja
AU  - Baščarević, Zvezdana
AU  - Ilić, Svetlana
AU  - Valenta-Šobot, Ana
AU  - Šaponjić, Aleksandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11306
PB  - Academy of Sciences and Arts of the Republic of Srpska
C3  - „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts
T1  - Synthesis and characterisation of SBA-15 with spherical particles
SP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11306
ER  - 

@conference{
author = "Kokunešoski, Maja and Baščarević, Zvezdana and Ilić, Svetlana and Valenta-Šobot, Ana and Šaponjić, Aleksandra",
year = "2021",
publisher = "Academy of Sciences and Arts of the Republic of Srpska",
journal = "„Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts",
title = "Synthesis and characterisation of SBA-15 with spherical particles",
pages = "48",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11306"
}

Kokunešoski, M., Baščarević, Z., Ilić, S., Valenta-Šobot, A.,& Šaponjić, A.. (2021). Synthesis and characterisation of SBA-15 with spherical particles. in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts
Academy of Sciences and Arts of the Republic of Srpska., 48.
https://hdl.handle.net/21.15107/rcub_vinar_11306

Kokunešoski M, Baščarević Z, Ilić S, Valenta-Šobot A, Šaponjić A. Synthesis and characterisation of SBA-15 with spherical particles. in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts. 2021;:48.
https://hdl.handle.net/21.15107/rcub_vinar_11306 .

Kokunešoski, Maja, Baščarević, Zvezdana, Ilić, Svetlana, Valenta-Šobot, Ana, Šaponjić, Aleksandra, "Synthesis and characterisation of SBA-15 with spherical particles" in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts (2021):48,
https://hdl.handle.net/21.15107/rcub_vinar_11306 .

TY  - CONF
AU  - Kokunešoski, Maja
AU  - Bašćarević, Zvezdana
AU  - Ilić, Svetlana
AU  - Valenta-Šobot, Ana
AU  - Grce, Ana
AU  - Pošarac-Marković, Milica B.
AU  - Šaponjić, Aleksandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10799
AB  - The synthesis of SBA-15 material with spherical particles is performed by the template method by using only a surfactant Pluronic P123 under acidic conditions. In the synthesis of SBA-15 with spherical particles, an HCl solution was used after specialised chemical treatment of clay purification. The dominant presence of the spheres with diameters up to around 2 μm was confirmed by the scanning electron microscopy(SEM) method. In contrast, the Energy-dispersive X-ray spectroscopy(EDS) confirmed that the spheres consisted only of SiO2 in composition. In addition to the methods mentioned above, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy(FTIR) methods were used to characterise SBA- 15 materials. Application of HCl solution after chemical treatment of clay purification represents the application of technology in the synthesis of spherical SBA-15.
PB  - Belgrade : Serbian Ceramic Society
C3  - Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade
T1  - Synthesis of spherical SBA-15 silica particles without the use of additional cosurfactant
SP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10799
ER  - 

@conference{
author = "Kokunešoski, Maja and Bašćarević, Zvezdana and Ilić, Svetlana and Valenta-Šobot, Ana and Grce, Ana and Pošarac-Marković, Milica B. and Šaponjić, Aleksandra",
year = "2021",
abstract = "The synthesis of SBA-15 material with spherical particles is performed by the template method by using only a surfactant Pluronic P123 under acidic conditions. In the synthesis of SBA-15 with spherical particles, an HCl solution was used after specialised chemical treatment of clay purification. The dominant presence of the spheres with diameters up to around 2 μm was confirmed by the scanning electron microscopy(SEM) method. In contrast, the Energy-dispersive X-ray spectroscopy(EDS) confirmed that the spheres consisted only of SiO2 in composition. In addition to the methods mentioned above, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy(FTIR) methods were used to characterise SBA- 15 materials. Application of HCl solution after chemical treatment of clay purification represents the application of technology in the synthesis of spherical SBA-15.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade",
title = "Synthesis of spherical SBA-15 silica particles without the use of additional cosurfactant",
pages = "66",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10799"
}

Kokunešoski, M., Bašćarević, Z., Ilić, S., Valenta-Šobot, A., Grce, A., Pošarac-Marković, M. B.,& Šaponjić, A.. (2021). Synthesis of spherical SBA-15 silica particles without the use of additional cosurfactant. in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade
Belgrade : Serbian Ceramic Society., 66.
https://hdl.handle.net/21.15107/rcub_vinar_10799

Kokunešoski M, Bašćarević Z, Ilić S, Valenta-Šobot A, Grce A, Pošarac-Marković MB, Šaponjić A. Synthesis of spherical SBA-15 silica particles without the use of additional cosurfactant. in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade. 2021;:66.
https://hdl.handle.net/21.15107/rcub_vinar_10799 .

Kokunešoski, Maja, Bašćarević, Zvezdana, Ilić, Svetlana, Valenta-Šobot, Ana, Grce, Ana, Pošarac-Marković, Milica B., Šaponjić, Aleksandra, "Synthesis of spherical SBA-15 silica particles without the use of additional cosurfactant" in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade (2021):66,
https://hdl.handle.net/21.15107/rcub_vinar_10799 .

TY  - JOUR
AU  - Valenta-Šobot, Ana
AU  - Savić, Jasmina
AU  - Filipović Tričković, Jelena G.
AU  - Drakulić, Dunja R.
AU  - Joksić, Gordana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10254
AB  - Root of Gentiana lutea commercially available as gentian root, a natural antidote for different types of poisons, possess antioxidative, immunomodulatory, cytoprotective and anti-inflammatory, and adverse, genotoxic and mutagenic effects. It has monoterpenes loganic acid, swertiamarin, gentiopicroside and sweroside as most abundant constituents. In this study, we assessed the toxicity of monoterpenes’ reactive molecular fragments using in silico prediction by VEGA-QSAR platform. Further, we compared the data obtained with in vitro geno- and cyto- toxicity testing of the above monoterpenes and the G. lutea root extract (GE), on human primary unstimulated and mitogen-stimulated peripheral blood mononuclear cells (PBMCs). Viability was assessed by TB and XTT tests after 48 h treatment. DNA damage was evaluated by alkaline comet assay on unstimulated cells, whereas cytokinesis-block micronucleus assay was employed on mitogen-stimulated PBMCs. Stability of compounds throughout treatment was monitored by UPLC. The observed in vitro results had highest compliance with in silico IRFMN/ISSCAN-CGX prediction model. Compounds showed high stability during experiment while treatment with single compounds reduced number of viable cells and increased DNA damage. GE treatment had toxic impact on unstimulated PBMCs but no significant genotoxic influence on mitogen-stimulated PBMCs. In summary, the mild GE effect suggests that the complexity of crude GE extract chemical composition  may attenuate the toxicity of the tested monoterpenes loganic acid, swertiamarin, gentiopicroside and sweroside.
T2  - Indian Journal of Experimental Biology
T1  - Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds
VL  - 58
IS  - 9
SP  - 609
EP  - 616
DO  - 10.56042/ijeb.v58i09.39874
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10254
ER  - 

@article{
author = "Valenta-Šobot, Ana and Savić, Jasmina and Filipović Tričković, Jelena G. and Drakulić, Dunja R. and Joksić, Gordana",
year = "2020",
abstract = "Root of Gentiana lutea commercially available as gentian root, a natural antidote for different types of poisons, possess antioxidative, immunomodulatory, cytoprotective and anti-inflammatory, and adverse, genotoxic and mutagenic effects. It has monoterpenes loganic acid, swertiamarin, gentiopicroside and sweroside as most abundant constituents. In this study, we assessed the toxicity of monoterpenes’ reactive molecular fragments using in silico prediction by VEGA-QSAR platform. Further, we compared the data obtained with in vitro geno- and cyto- toxicity testing of the above monoterpenes and the G. lutea root extract (GE), on human primary unstimulated and mitogen-stimulated peripheral blood mononuclear cells (PBMCs). Viability was assessed by TB and XTT tests after 48 h treatment. DNA damage was evaluated by alkaline comet assay on unstimulated cells, whereas cytokinesis-block micronucleus assay was employed on mitogen-stimulated PBMCs. Stability of compounds throughout treatment was monitored by UPLC. The observed in vitro results had highest compliance with in silico IRFMN/ISSCAN-CGX prediction model. Compounds showed high stability during experiment while treatment with single compounds reduced number of viable cells and increased DNA damage. GE treatment had toxic impact on unstimulated PBMCs but no significant genotoxic influence on mitogen-stimulated PBMCs. In summary, the mild GE effect suggests that the complexity of crude GE extract chemical composition  may attenuate the toxicity of the tested monoterpenes loganic acid, swertiamarin, gentiopicroside and sweroside.",
journal = "Indian Journal of Experimental Biology",
title = "Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds",
volume = "58",
number = "9",
pages = "609-616",
doi = "10.56042/ijeb.v58i09.39874",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10254"
}

Valenta-Šobot, A., Savić, J., Filipović Tričković, J. G., Drakulić, D. R.,& Joksić, G.. (2020). Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds. in Indian Journal of Experimental Biology, 58(9), 609-616.
https://doi.org/10.56042/ijeb.v58i09.39874
https://hdl.handle.net/21.15107/rcub_vinar_10254

Valenta-Šobot A, Savić J, Filipović Tričković JG, Drakulić DR, Joksić G. Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds. in Indian Journal of Experimental Biology. 2020;58(9):609-616.
doi:10.56042/ijeb.v58i09.39874
https://hdl.handle.net/21.15107/rcub_vinar_10254 .

Valenta-Šobot, Ana, Savić, Jasmina, Filipović Tričković, Jelena G., Drakulić, Dunja R., Joksić, Gordana, "Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds" in Indian Journal of Experimental Biology, 58, no. 9 (2020):609-616,
https://doi.org/10.56042/ijeb.v58i09.39874 .,
https://hdl.handle.net/21.15107/rcub_vinar_10254 .

TY  - JOUR
AU  - Valenta-Šobot, Ana
AU  - Drakulić, Dunja R.
AU  - Joksić, Gordana
AU  - Miletić Vukajlović, Jadranka
AU  - Savić, Jasmina
AU  - Potočnik, Jelena
AU  - Filipović Tričković, Jelena G.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9523
AB  - Yellow gentian (Gentiana lutea L.), a medicinal plant widely used in traditional medicine, displays multiple biological effects, ranging from beneficial to toxic. Since many promising applications have been reported so far, our aim was to evaluate its potential concentration- and time- dependent cytotoxic and genotoxic effects in vitro . To that end we exposed human peripheral blood mononuclear cells to 0.5, 1, and 2 mg/mL of yellow gentian root extract (YGRE) to determine its effects on oxidative stress parameters [pro/antioxidant balance (PAB) and lipid peroxidation], DNA damage (alkaline comet assay and chromosome aberrations), and cell viability (trypan blue exclusion test). Cell viability decreased with increasing concentrations and treatment duration. Only the lowest YGRE concentration (0.5 mg/mL) increased oxidative stress but produced minor DNA damage and cytotoxicity. At higher concentrations, redox parameters returned to near control values. The percentage of chromosome aberrations and percentage of DNA in the comet tail increased with increased YGRE concentration after 48 h and declined after 72 h of treatment. This points to the activation of DNA repair mechanism (homologous recombination), evidenced by the formation of chromosomal radial figures after 72 h of treatment with the highest YGRE concentration of 2 mg/mL. Our results suggest that YGRE, despite induction of cytotoxic and genotoxic effects, activates cell repair mechanisms that counter oxidative and DNA lesions and induce cell death in highly damaged cells. Therefore, observed protective effects of yellow gentian after longer exposure could be a result of activated repair and removal of cells with irreparable damage.
T2  - Archives of Industrial Hygiene and Toxicology
T1  - Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells
VL  - 71
IS  - 4
SP  - 320
EP  - 328
DO  - 10.2478/aiht-2020-71-3476
ER  - 

@article{
author = "Valenta-Šobot, Ana and Drakulić, Dunja R. and Joksić, Gordana and Miletić Vukajlović, Jadranka and Savić, Jasmina and Potočnik, Jelena and Filipović Tričković, Jelena G.",
year = "2020",
abstract = "Yellow gentian (Gentiana lutea L.), a medicinal plant widely used in traditional medicine, displays multiple biological effects, ranging from beneficial to toxic. Since many promising applications have been reported so far, our aim was to evaluate its potential concentration- and time- dependent cytotoxic and genotoxic effects in vitro . To that end we exposed human peripheral blood mononuclear cells to 0.5, 1, and 2 mg/mL of yellow gentian root extract (YGRE) to determine its effects on oxidative stress parameters [pro/antioxidant balance (PAB) and lipid peroxidation], DNA damage (alkaline comet assay and chromosome aberrations), and cell viability (trypan blue exclusion test). Cell viability decreased with increasing concentrations and treatment duration. Only the lowest YGRE concentration (0.5 mg/mL) increased oxidative stress but produced minor DNA damage and cytotoxicity. At higher concentrations, redox parameters returned to near control values. The percentage of chromosome aberrations and percentage of DNA in the comet tail increased with increased YGRE concentration after 48 h and declined after 72 h of treatment. This points to the activation of DNA repair mechanism (homologous recombination), evidenced by the formation of chromosomal radial figures after 72 h of treatment with the highest YGRE concentration of 2 mg/mL. Our results suggest that YGRE, despite induction of cytotoxic and genotoxic effects, activates cell repair mechanisms that counter oxidative and DNA lesions and induce cell death in highly damaged cells. Therefore, observed protective effects of yellow gentian after longer exposure could be a result of activated repair and removal of cells with irreparable damage.",
journal = "Archives of Industrial Hygiene and Toxicology",
title = "Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells",
volume = "71",
number = "4",
pages = "320-328",
doi = "10.2478/aiht-2020-71-3476"
}

Valenta-Šobot, A., Drakulić, D. R., Joksić, G., Miletić Vukajlović, J., Savić, J., Potočnik, J.,& Filipović Tričković, J. G.. (2020). Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells. in Archives of Industrial Hygiene and Toxicology, 71(4), 320-328.
https://doi.org/10.2478/aiht-2020-71-3476

Valenta-Šobot A, Drakulić DR, Joksić G, Miletić Vukajlović J, Savić J, Potočnik J, Filipović Tričković JG. Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells. in Archives of Industrial Hygiene and Toxicology. 2020;71(4):320-328.
doi:10.2478/aiht-2020-71-3476 .

Valenta-Šobot, Ana, Drakulić, Dunja R., Joksić, Gordana, Miletić Vukajlović, Jadranka, Savić, Jasmina, Potočnik, Jelena, Filipović Tričković, Jelena G., "Yellow gentian root extract provokes concentration- and time-dependent response in peripheral blood mononuclear cells" in Archives of Industrial Hygiene and Toxicology, 71, no. 4 (2020):320-328,
https://doi.org/10.2478/aiht-2020-71-3476 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Filipović Tričković, Jelena G.
AU  - Mićić, Mileva
AU  - Joksić, Ivana
AU  - Valenta-Šobot, Ana
AU  - Demajo, Miroslav
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9729
AB  - Traditional methods in cell proliferation studies are based on immunohistochemical detection of proliferating cells in the target tissue. Since they are time consuming, optimization of novel, more efficient methods is important for large scale proliferation studies. In this study, we aimed to optimize the isolation of single epithelial rat forestomach cells for flow cytometry. As a marker of cellular proliferation we used the Ki-67 antibody to detect this nuclear protein expressed in proliferating cells. We also performed immunohistochemical detection of Ki-67 positive cells and propidium iodide staining to validate the results. 3-tert-butyl-4-hydroxyanisole was used as the positive control to ensure cellular proliferation. The results showed that isolation of epithelial cells with collagenase, trypsin and cell strainer ensures great cell viability (>95%) and the purity of the samples. Flow cytometry and immunostaining with the Ki-67 antibody indicated that 3-tert-butyl-4-hydroxyanisole treatment leads to a significant increase in proliferation. A significant positive correlation was observed between the results obtained by immunohistochemistry and flow cytometry, but the flow cytometric data had a smaller measurement error, suggesting the equal sensitivity and greater accuracy of this method. Propidium iodide staining showed that the percentage of cells in the G2+S phase of the cell cycle correlated positively with the percentage of Ki-67 positive cells assessed by flow cytometry, indicating that Ki-67 positive cells reflect an active dividing cell pool. We conclude that the isolation of forestomach epithelial cells described is a simple and reliable method for obtaining viable cells for use in flow cytometry. Compared to immunohistochemistry, flow cytometric detection of the Ki-67 antigen is equally sensitive, but much faster and provides more accurate results. © 2020, University of Zagreb, Facultty of Veterinary Medicine. All rights reserved.
T2  - Veterinarski Arhiv
T1  - Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry
VL  - 90
IS  - 5
SP  - 517
EP  - 525
DO  - 10.24099/vet.arhiv.0956
ER  - 

@article{
author = "Joksić, Gordana and Filipović Tričković, Jelena G. and Mićić, Mileva and Joksić, Ivana and Valenta-Šobot, Ana and Demajo, Miroslav",
year = "2020",
abstract = "Traditional methods in cell proliferation studies are based on immunohistochemical detection of proliferating cells in the target tissue. Since they are time consuming, optimization of novel, more efficient methods is important for large scale proliferation studies. In this study, we aimed to optimize the isolation of single epithelial rat forestomach cells for flow cytometry. As a marker of cellular proliferation we used the Ki-67 antibody to detect this nuclear protein expressed in proliferating cells. We also performed immunohistochemical detection of Ki-67 positive cells and propidium iodide staining to validate the results. 3-tert-butyl-4-hydroxyanisole was used as the positive control to ensure cellular proliferation. The results showed that isolation of epithelial cells with collagenase, trypsin and cell strainer ensures great cell viability (>95%) and the purity of the samples. Flow cytometry and immunostaining with the Ki-67 antibody indicated that 3-tert-butyl-4-hydroxyanisole treatment leads to a significant increase in proliferation. A significant positive correlation was observed between the results obtained by immunohistochemistry and flow cytometry, but the flow cytometric data had a smaller measurement error, suggesting the equal sensitivity and greater accuracy of this method. Propidium iodide staining showed that the percentage of cells in the G2+S phase of the cell cycle correlated positively with the percentage of Ki-67 positive cells assessed by flow cytometry, indicating that Ki-67 positive cells reflect an active dividing cell pool. We conclude that the isolation of forestomach epithelial cells described is a simple and reliable method for obtaining viable cells for use in flow cytometry. Compared to immunohistochemistry, flow cytometric detection of the Ki-67 antigen is equally sensitive, but much faster and provides more accurate results. © 2020, University of Zagreb, Facultty of Veterinary Medicine. All rights reserved.",
journal = "Veterinarski Arhiv",
title = "Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry",
volume = "90",
number = "5",
pages = "517-525",
doi = "10.24099/vet.arhiv.0956"
}

Joksić, G., Filipović Tričković, J. G., Mićić, M., Joksić, I., Valenta-Šobot, A.,& Demajo, M.. (2020). Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry. in Veterinarski Arhiv, 90(5), 517-525.
https://doi.org/10.24099/vet.arhiv.0956

Joksić G, Filipović Tričković JG, Mićić M, Joksić I, Valenta-Šobot A, Demajo M. Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry. in Veterinarski Arhiv. 2020;90(5):517-525.
doi:10.24099/vet.arhiv.0956 .

Joksić, Gordana, Filipović Tričković, Jelena G., Mićić, Mileva, Joksić, Ivana, Valenta-Šobot, Ana, Demajo, Miroslav, "Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry" in Veterinarski Arhiv, 90, no. 5 (2020):517-525,
https://doi.org/10.24099/vet.arhiv.0956 . .

TY  - JOUR
AU  - Miletić Vukajlović, Jadranka
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Valenta-Šobot, Ana
AU  - Drakulić, Dunja R.
AU  - Pavlović, Ivan
AU  - Stefanović, Aleksandra
AU  - Prostran, Milica
AU  - Ilić, Tihomir V.
AU  - Stojanov, Marina
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8407
AB  - Backgroun / Aim. Constant production of free radicals and antioxidants (AO) in the cell is a part of normal cellular function. Their imbalance might take a part in pathophysiology of many diseases, including Parkinson’s disease (PD). Evaluation of the disease status, prooxidant-antioxidant balance (PAB) and antioxidants are being widely estimated. The aim of this study was to examine potential interaction between several AO variables (GSH, SOD, CAT and PAB) and clinicopathological features of patients with PD, particularly Hoehn and Yahr (H&Y) stage. Methods. A multivariate analysis of variance (MANOVA) was conducted to test the hypothesis of the mean differences between clinicopathological characteristics (gender, age at examination, duration of the disease, and H&Y stage) and AO variables, compared with age/sex matched healthy controls. The study included 91 patients with idiopatic PD patients and 20 healthy controls. Results. The multivariate effect size was estimated at 0.269, p <0.001, implying that 27.0% of the variance of the dependent variables was accounted for H&Y stage. Univariate tests showed that there were significant differences (p <0.001) across the H&Y stage on all AO variables. The H&Y stage remained significant predictor after controlling for the second variable, the disease duration (p <0.001, η2 = 0.249), and there were still significant differences across the H&Y stage on all variables, with effect size ( η2) ranging from 0.132, p =0.011 (lnGSH) to the still high values of 0.535 (lnPAB), 0.627 (lnSOD) and 0.964 (lnCAT). Conclusion. The results indicate that higher level of oxidative stress in blood of PD patients is possibly related to PD stage. Along with reduction of SOD and GSH level, CAT activity was elevated in comparison to healthy subjects. Furthermore, Pwas shifted toward oxidative stress.
T2  - Vojnosanitetski pregled
T1  - Antioxidant status and clinicopathological parameters in patients with Parkinson's disease
VL  - 77
IS  - 7
SP  - 724
EP  - 730
DO  - 10.2298/VSP180718148M
ER  - 

@article{
author = "Miletić Vukajlović, Jadranka and Pejić, Snežana and Todorović, Ana and Valenta-Šobot, Ana and Drakulić, Dunja R. and Pavlović, Ivan and Stefanović, Aleksandra and Prostran, Milica and Ilić, Tihomir V. and Stojanov, Marina",
year = "2019",
abstract = "Backgroun / Aim. Constant production of free radicals and antioxidants (AO) in the cell is a part of normal cellular function. Their imbalance might take a part in pathophysiology of many diseases, including Parkinson’s disease (PD). Evaluation of the disease status, prooxidant-antioxidant balance (PAB) and antioxidants are being widely estimated. The aim of this study was to examine potential interaction between several AO variables (GSH, SOD, CAT and PAB) and clinicopathological features of patients with PD, particularly Hoehn and Yahr (H&Y) stage. Methods. A multivariate analysis of variance (MANOVA) was conducted to test the hypothesis of the mean differences between clinicopathological characteristics (gender, age at examination, duration of the disease, and H&Y stage) and AO variables, compared with age/sex matched healthy controls. The study included 91 patients with idiopatic PD patients and 20 healthy controls. Results. The multivariate effect size was estimated at 0.269, p <0.001, implying that 27.0% of the variance of the dependent variables was accounted for H&Y stage. Univariate tests showed that there were significant differences (p <0.001) across the H&Y stage on all AO variables. The H&Y stage remained significant predictor after controlling for the second variable, the disease duration (p <0.001, η2 = 0.249), and there were still significant differences across the H&Y stage on all variables, with effect size ( η2) ranging from 0.132, p =0.011 (lnGSH) to the still high values of 0.535 (lnPAB), 0.627 (lnSOD) and 0.964 (lnCAT). Conclusion. The results indicate that higher level of oxidative stress in blood of PD patients is possibly related to PD stage. Along with reduction of SOD and GSH level, CAT activity was elevated in comparison to healthy subjects. Furthermore, Pwas shifted toward oxidative stress.",
journal = "Vojnosanitetski pregled",
title = "Antioxidant status and clinicopathological parameters in patients with Parkinson's disease",
volume = "77",
number = "7",
pages = "724-730",
doi = "10.2298/VSP180718148M"
}

Miletić Vukajlović, J., Pejić, S., Todorović, A., Valenta-Šobot, A., Drakulić, D. R., Pavlović, I., Stefanović, A., Prostran, M., Ilić, T. V.,& Stojanov, M.. (2019). Antioxidant status and clinicopathological parameters in patients with Parkinson's disease. in Vojnosanitetski pregled, 77(7), 724-730.
https://doi.org/10.2298/VSP180718148M

Miletić Vukajlović J, Pejić S, Todorović A, Valenta-Šobot A, Drakulić DR, Pavlović I, Stefanović A, Prostran M, Ilić TV, Stojanov M. Antioxidant status and clinicopathological parameters in patients with Parkinson's disease. in Vojnosanitetski pregled. 2019;77(7):724-730.
doi:10.2298/VSP180718148M .

Miletić Vukajlović, Jadranka, Pejić, Snežana, Todorović, Ana, Valenta-Šobot, Ana, Drakulić, Dunja R., Pavlović, Ivan, Stefanović, Aleksandra, Prostran, Milica, Ilić, Tihomir V., Stojanov, Marina, "Antioxidant status and clinicopathological parameters in patients with Parkinson's disease" in Vojnosanitetski pregled, 77, no. 7 (2019):724-730,
https://doi.org/10.2298/VSP180718148M . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Valenta-Šobot, Ana
AU  - Filipović Tričković, Jelena G.
AU  - Maletić, Dejan
AU  - Puač, Nevena
AU  - Malović, Gordana N.
AU  - Petrović, Zoran Lj.
AU  - Lazović, Saša
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8178
AB  - In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4-1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of 2-3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h. © 2019, Indian Academy of Sciences.
T2  - Current Science
T1  - Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation
VL  - 116
IS  - 7
SP  - 1229
EP  - 1233
DO  - 10.18520/cs/v116/i7/1229-1233
ER  - 

@article{
author = "Joksić, Gordana and Valenta-Šobot, Ana and Filipović Tričković, Jelena G. and Maletić, Dejan and Puač, Nevena and Malović, Gordana N. and Petrović, Zoran Lj. and Lazović, Saša",
year = "2019",
abstract = "In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4-1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of 2-3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h. © 2019, Indian Academy of Sciences.",
journal = "Current Science",
title = "Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation",
volume = "116",
number = "7",
pages = "1229-1233",
doi = "10.18520/cs/v116/i7/1229-1233"
}

Joksić, G., Valenta-Šobot, A., Filipović Tričković, J. G., Maletić, D., Puač, N., Malović, G. N., Petrović, Z. Lj.,& Lazović, S.. (2019). Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation. in Current Science, 116(7), 1229-1233.
https://doi.org/10.18520/cs/v116/i7/1229-1233

Joksić G, Valenta-Šobot A, Filipović Tričković JG, Maletić D, Puač N, Malović GN, Petrović ZL, Lazović S. Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation. in Current Science. 2019;116(7):1229-1233.
doi:10.18520/cs/v116/i7/1229-1233 .

Joksić, Gordana, Valenta-Šobot, Ana, Filipović Tričković, Jelena G., Maletić, Dejan, Puač, Nevena, Malović, Gordana N., Petrović, Zoran Lj., Lazović, Saša, "Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation" in Current Science, 116, no. 7 (2019):1229-1233,
https://doi.org/10.18520/cs/v116/i7/1229-1233 . .

TY  - JOUR
AU  - Filipović Tričković, Jelena G.
AU  - Mandušić, Vesna
AU  - Joksić, Ivana
AU  - Vujić, Dragana
AU  - Valenta-Šobot, Ana
AU  - Joksić, Gordana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1882
AB  - Fanconi anemia is rare inherited disease characterized by wide spectrum of congenital anomalies, progressive pancytopenia, and predisposition to hematological malignancies and solid tumors. Molecular genetic analysis of mutations in FANC genes is of a great importance for diagnosis confirmation, prenatal and carrier testing, as well as for prediction of chemotherapy outcome and disease complications. In this study we performed screening of frequently affected regions of FANCD2 gene for sequence variants in six unrelated FA-D2 patients in Serbia. This is the first molecular analysis of FANCD2 gene in Serbian FA-D2 patients. A total of 10 sequence variants were detected, one in homozygous, and nine in heterozygous state. Two variants were found within exons, and eight within introns, in deep intronic regions. In-silico analysis showed that among all detected variants one exon variant and three intron variants might have impact on splicing mechanism. Heterozygous variants found in intron 3, c. 206-246delG; exon 26, c. 2396 C GT A and intron 28, c. 2715+573 C GT T were not previously reported. In-silico analysis revealed that among them, two (intron 3, c. 206-246 delG and exon 26, c. 2396 C GT A) could be novel disease-causing mutations. Many variants were found in more than one patient, including those unreported, indicating their possible ethnic association. Great number of variants in some patients suggests their non-random emergence in Fanconi anemia pathway.
T2  - Genetika
T1  - Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants
VL  - 49
IS  - 2
SP  - 559
EP  - 572
DO  - 10.2298/GENSR1702559T
ER  - 

@article{
author = "Filipović Tričković, Jelena G. and Mandušić, Vesna and Joksić, Ivana and Vujić, Dragana and Valenta-Šobot, Ana and Joksić, Gordana",
year = "2017",
abstract = "Fanconi anemia is rare inherited disease characterized by wide spectrum of congenital anomalies, progressive pancytopenia, and predisposition to hematological malignancies and solid tumors. Molecular genetic analysis of mutations in FANC genes is of a great importance for diagnosis confirmation, prenatal and carrier testing, as well as for prediction of chemotherapy outcome and disease complications. In this study we performed screening of frequently affected regions of FANCD2 gene for sequence variants in six unrelated FA-D2 patients in Serbia. This is the first molecular analysis of FANCD2 gene in Serbian FA-D2 patients. A total of 10 sequence variants were detected, one in homozygous, and nine in heterozygous state. Two variants were found within exons, and eight within introns, in deep intronic regions. In-silico analysis showed that among all detected variants one exon variant and three intron variants might have impact on splicing mechanism. Heterozygous variants found in intron 3, c. 206-246delG; exon 26, c. 2396 C GT A and intron 28, c. 2715+573 C GT T were not previously reported. In-silico analysis revealed that among them, two (intron 3, c. 206-246 delG and exon 26, c. 2396 C GT A) could be novel disease-causing mutations. Many variants were found in more than one patient, including those unreported, indicating their possible ethnic association. Great number of variants in some patients suggests their non-random emergence in Fanconi anemia pathway.",
journal = "Genetika",
title = "Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants",
volume = "49",
number = "2",
pages = "559-572",
doi = "10.2298/GENSR1702559T"
}

Filipović Tričković, J. G., Mandušić, V., Joksić, I., Vujić, D., Valenta-Šobot, A.,& Joksić, G.. (2017). Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants. in Genetika, 49(2), 559-572.
https://doi.org/10.2298/GENSR1702559T

Filipović Tričković JG, Mandušić V, Joksić I, Vujić D, Valenta-Šobot A, Joksić G. Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants. in Genetika. 2017;49(2):559-572.
doi:10.2298/GENSR1702559T .

Filipović Tričković, Jelena G., Mandušić, Vesna, Joksić, Ivana, Vujić, Dragana, Valenta-Šobot, Ana, Joksić, Gordana, "Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants" in Genetika, 49, no. 2 (2017):559-572,
https://doi.org/10.2298/GENSR1702559T . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Mićić, Mileva
AU  - Filipović, Jelena G.
AU  - Drakulić, Dunja R.
AU  - Stanojlović, Miloš R.
AU  - Calija, Bojan
AU  - Valenta-Šobot, Ana
AU  - Demajo, Miroslav
AU  - Nilsson, Robert
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1584
AB  - The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.
T2  - Acta Veterinaria, Beograd
T1  - Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach
VL  - 67
IS  - 1
SP  - 1
EP  - 10
DO  - 10.1515/acve-2017-0001
ER  - 

@article{
author = "Joksić, Gordana and Mićić, Mileva and Filipović, Jelena G. and Drakulić, Dunja R. and Stanojlović, Miloš R. and Calija, Bojan and Valenta-Šobot, Ana and Demajo, Miroslav and Nilsson, Robert",
year = "2017",
abstract = "The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.",
journal = "Acta Veterinaria, Beograd",
title = "Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach",
volume = "67",
number = "1",
pages = "1-10",
doi = "10.1515/acve-2017-0001"
}

Joksić, G., Mićić, M., Filipović, J. G., Drakulić, D. R., Stanojlović, M. R., Calija, B., Valenta-Šobot, A., Demajo, M.,& Nilsson, R.. (2017). Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach. in Acta Veterinaria, Beograd, 67(1), 1-10.
https://doi.org/10.1515/acve-2017-0001

Joksić G, Mićić M, Filipović JG, Drakulić DR, Stanojlović MR, Calija B, Valenta-Šobot A, Demajo M, Nilsson R. Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach. in Acta Veterinaria, Beograd. 2017;67(1):1-10.
doi:10.1515/acve-2017-0001 .

Joksić, Gordana, Mićić, Mileva, Filipović, Jelena G., Drakulić, Dunja R., Stanojlović, Miloš R., Calija, Bojan, Valenta-Šobot, Ana, Demajo, Miroslav, Nilsson, Robert, "Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach" in Acta Veterinaria, Beograd, 67, no. 1 (2017):1-10,
https://doi.org/10.1515/acve-2017-0001 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Stašić, Jelena
AU  - Filipović, Jelena G.
AU  - Valenta-Šobot, Ana
AU  - Trtica, Milan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/976
AB  - In this work we present biological effects of silver nanoparticles (AgNPs) synthesized by picosecond laser ablation of silver in deionized water. We examined induction of chromosomal aberrations, lymphocyte micronuclei, appearance and recovery of double strand breaks (DSBs) of DNA, cell proliferation potential, concentration of lipid peroxidation products and insulin-like growth factor 1 (ILGF-1). We found that AgNPs sized from 3 nm to 8 nm induce cell cytostasis, which is accompanied with its clastogenic action on DNA, while AgNPs, sized 2 nm behaves contrary stimulating cell proliferation by enhancing ILGF-1 concentration. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
PB  - Elsevier
T2  - Toxicology Letters
T1  - Size of silver nanoparticles determines proliferation ability of human circulating lymphocytes in vitro
VL  - 247
SP  - 29
EP  - 34
DO  - 10.1016/j.toxlet.2016.02.007
ER  - 

@article{
author = "Joksić, Gordana and Stašić, Jelena and Filipović, Jelena G. and Valenta-Šobot, Ana and Trtica, Milan",
year = "2016",
abstract = "In this work we present biological effects of silver nanoparticles (AgNPs) synthesized by picosecond laser ablation of silver in deionized water. We examined induction of chromosomal aberrations, lymphocyte micronuclei, appearance and recovery of double strand breaks (DSBs) of DNA, cell proliferation potential, concentration of lipid peroxidation products and insulin-like growth factor 1 (ILGF-1). We found that AgNPs sized from 3 nm to 8 nm induce cell cytostasis, which is accompanied with its clastogenic action on DNA, while AgNPs, sized 2 nm behaves contrary stimulating cell proliferation by enhancing ILGF-1 concentration. (C) 2016 Elsevier Ireland Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Toxicology Letters",
title = "Size of silver nanoparticles determines proliferation ability of human circulating lymphocytes in vitro",
volume = "247",
pages = "29-34",
doi = "10.1016/j.toxlet.2016.02.007"
}

Joksić, G., Stašić, J., Filipović, J. G., Valenta-Šobot, A.,& Trtica, M.. (2016). Size of silver nanoparticles determines proliferation ability of human circulating lymphocytes in vitro. in Toxicology Letters
Elsevier., 247, 29-34.
https://doi.org/10.1016/j.toxlet.2016.02.007

Joksić G, Stašić J, Filipović JG, Valenta-Šobot A, Trtica M. Size of silver nanoparticles determines proliferation ability of human circulating lymphocytes in vitro. in Toxicology Letters. 2016;247:29-34.
doi:10.1016/j.toxlet.2016.02.007 .

Joksić, Gordana, Stašić, Jelena, Filipović, Jelena G., Valenta-Šobot, Ana, Trtica, Milan, "Size of silver nanoparticles determines proliferation ability of human circulating lymphocytes in vitro" in Toxicology Letters, 247 (2016):29-34,
https://doi.org/10.1016/j.toxlet.2016.02.007 . .