TY  - JOUR
AU  - Kamceva, Tina
AU  - Radisavljević, Maja
AU  - Vukićević, Iva A.
AU  - Arnhold, Juergen
AU  - Petković, Marijana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5790
AB  - Phospholipase A(2) is involved in propagation of inflammatory processes and carcinogenesis through its role in phospholipid metabolism, and release of arachidonic acid and lysophospholipids. Recent findings on correlation between elevated PLA(2) activity and metastatic cancer render this enzyme an attractive target for cancer therapy. On the other hand, due to a broad range of oxidation states under physiological conditions and a high affinity for protein binding, platinum and ruthenium coordination complexes are promising candidates for PLA(2) inhibitors. In this article, we discuss the interactions of Pt and Ru coordination complexes with PLA(2) and phospholipids, as well as the application of MALDI-TOF mass spectrometry for screening PLA(2) inhibitors. Owing to the ability of this technique to simultaneously detect and monitor changes in substrate and product concentrations, the inhibitor mechanisms of both Pt and Ru complexes with various ligands were determined.
T2  - Chemistry and Biodiversity
T1  - Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry
VL  - 10
IS  - 11
SP  - 1972
EP  - 1986
DO  - 10.1002/cbdv.201300141
ER  - 

@article{
author = "Kamceva, Tina and Radisavljević, Maja and Vukićević, Iva A. and Arnhold, Juergen and Petković, Marijana",
year = "2013",
abstract = "Phospholipase A(2) is involved in propagation of inflammatory processes and carcinogenesis through its role in phospholipid metabolism, and release of arachidonic acid and lysophospholipids. Recent findings on correlation between elevated PLA(2) activity and metastatic cancer render this enzyme an attractive target for cancer therapy. On the other hand, due to a broad range of oxidation states under physiological conditions and a high affinity for protein binding, platinum and ruthenium coordination complexes are promising candidates for PLA(2) inhibitors. In this article, we discuss the interactions of Pt and Ru coordination complexes with PLA(2) and phospholipids, as well as the application of MALDI-TOF mass spectrometry for screening PLA(2) inhibitors. Owing to the ability of this technique to simultaneously detect and monitor changes in substrate and product concentrations, the inhibitor mechanisms of both Pt and Ru complexes with various ligands were determined.",
journal = "Chemistry and Biodiversity",
title = "Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry",
volume = "10",
number = "11",
pages = "1972-1986",
doi = "10.1002/cbdv.201300141"
}

Kamceva, T., Radisavljević, M., Vukićević, I. A., Arnhold, J.,& Petković, M.. (2013). Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry. in Chemistry and Biodiversity, 10(11), 1972-1986.
https://doi.org/10.1002/cbdv.201300141

Kamceva T, Radisavljević M, Vukićević IA, Arnhold J, Petković M. Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry. in Chemistry and Biodiversity. 2013;10(11):1972-1986.
doi:10.1002/cbdv.201300141 .

Kamceva, Tina, Radisavljević, Maja, Vukićević, Iva A., Arnhold, Juergen, Petković, Marijana, "Interactions of Platinum and Ruthenium Coordination Complexes with Pancreatic Phospholipase A(2) and Phospholipids Investigated by MALDI TOF Mass Spectrometry" in Chemistry and Biodiversity, 10, no. 11 (2013):1972-1986,
https://doi.org/10.1002/cbdv.201300141 . .

TY  - JOUR
AU  - Kamceva, Tina
AU  - Flemmig, Joerg
AU  - Damnjanović, Bojana
AU  - Arnhold, Juergen
AU  - Mijatovic, Aleksandar
AU  - Petković, Marijana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4514
AB  - Pancreatic phospholipase A(2) (PLA(2)) plays an important role in cellular homeostasis as well as in the process of carcinogenesis. Effects of metallo-drugs used as chemotherapeutics on the activity of this enzyme are unknown. In this work, the interaction between porcine pancreatic PLA(2) and two selected transition metal complexes-tetrachloro(bipyridine) platinum(IV) ([PtCl4(bipy)]) and dichloro (bipyridine) ruthenium(III)chloride ([RuCl2(bipy)(2)]Cl)-was studied. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and fluorescence spectroscopy have been used to analyse the enzyme activity in the absence and presence of metal complexes and to verify potential binding of these drugs to the enzyme. The tested metal complexes decreased the activity of phospholipase A(2) in an uncompetitive inhibition mode. A binding of the ruthenium complex near the active site of the enzyme could be evidenced and possible modes of interaction are discussed.
T2  - Metallomics
T1  - Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2)
VL  - 3
IS  - 10
SP  - 1056
EP  - 1063
DO  - 10.1039/c1mt00088h
ER  - 

@article{
author = "Kamceva, Tina and Flemmig, Joerg and Damnjanović, Bojana and Arnhold, Juergen and Mijatovic, Aleksandar and Petković, Marijana",
year = "2011",
abstract = "Pancreatic phospholipase A(2) (PLA(2)) plays an important role in cellular homeostasis as well as in the process of carcinogenesis. Effects of metallo-drugs used as chemotherapeutics on the activity of this enzyme are unknown. In this work, the interaction between porcine pancreatic PLA(2) and two selected transition metal complexes-tetrachloro(bipyridine) platinum(IV) ([PtCl4(bipy)]) and dichloro (bipyridine) ruthenium(III)chloride ([RuCl2(bipy)(2)]Cl)-was studied. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and fluorescence spectroscopy have been used to analyse the enzyme activity in the absence and presence of metal complexes and to verify potential binding of these drugs to the enzyme. The tested metal complexes decreased the activity of phospholipase A(2) in an uncompetitive inhibition mode. A binding of the ruthenium complex near the active site of the enzyme could be evidenced and possible modes of interaction are discussed.",
journal = "Metallomics",
title = "Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2)",
volume = "3",
number = "10",
pages = "1056-1063",
doi = "10.1039/c1mt00088h"
}

Kamceva, T., Flemmig, J., Damnjanović, B., Arnhold, J., Mijatovic, A.,& Petković, M.. (2011). Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2). in Metallomics, 3(10), 1056-1063.
https://doi.org/10.1039/c1mt00088h

Kamceva T, Flemmig J, Damnjanović B, Arnhold J, Mijatovic A, Petković M. Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2). in Metallomics. 2011;3(10):1056-1063.
doi:10.1039/c1mt00088h .

Kamceva, Tina, Flemmig, Joerg, Damnjanović, Bojana, Arnhold, Juergen, Mijatovic, Aleksandar, Petković, Marijana, "Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A(2)" in Metallomics, 3, no. 10 (2011):1056-1063,
https://doi.org/10.1039/c1mt00088h . .

TY  - JOUR
AU  - Petković, Marijana
AU  - Schiller, Jürgen
AU  - Mueller, Matthias
AU  - Suess, Rosmarie
AU  - Arnold, Klaus
AU  - Arnhold, Juergen
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3770
AB  - It is usually accepted that neutral phospholipids (PLs) generate singly positively charged ions, whereas negative PLs are easily detectable in the negative ion mode when analysed by matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry (MALDI-TOF MS). In this study, we demonstrate that some caution is required in the interpretation of MALDI-TOF mass spectra of PLs, since also neutral PLs have appeared to be detectable in the negative ion mode as well. Neutral and negatively charged phospholipids can generate adducts with the most commonly used matrix - 2,5-dihydroxybenzoic acid - yielding singly negatively charged ions that are detectable in the spectra. This further contributes to the complexity of the spectra and potentially leads to severe misinterpretation, particularly when unknown mixtures of PLs are analysed by MALDI-TOF MS.
T2  - Zeitschrift fur Naturforschung. Section B: Journal of Chemical Sciences
T1  - Detection of Adducts with Matrix Clusters in the Positive and Negative Ion Mode MALDI-TOF Mass Spectra of Phospholipids
VL  - 64
IS  - 3
SP  - 331
EP  - 334
DO  - 10.1515/znb-2009-0314
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3770
ER  - 

@article{
author = "Petković, Marijana and Schiller, Jürgen and Mueller, Matthias and Suess, Rosmarie and Arnold, Klaus and Arnhold, Juergen",
year = "2009",
abstract = "It is usually accepted that neutral phospholipids (PLs) generate singly positively charged ions, whereas negative PLs are easily detectable in the negative ion mode when analysed by matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry (MALDI-TOF MS). In this study, we demonstrate that some caution is required in the interpretation of MALDI-TOF mass spectra of PLs, since also neutral PLs have appeared to be detectable in the negative ion mode as well. Neutral and negatively charged phospholipids can generate adducts with the most commonly used matrix - 2,5-dihydroxybenzoic acid - yielding singly negatively charged ions that are detectable in the spectra. This further contributes to the complexity of the spectra and potentially leads to severe misinterpretation, particularly when unknown mixtures of PLs are analysed by MALDI-TOF MS.",
journal = "Zeitschrift fur Naturforschung. Section B: Journal of Chemical Sciences",
title = "Detection of Adducts with Matrix Clusters in the Positive and Negative Ion Mode MALDI-TOF Mass Spectra of Phospholipids",
volume = "64",
number = "3",
pages = "331-334",
doi = "10.1515/znb-2009-0314",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3770"
}

Petković, M., Schiller, J., Mueller, M., Suess, R., Arnold, K.,& Arnhold, J.. (2009). Detection of Adducts with Matrix Clusters in the Positive and Negative Ion Mode MALDI-TOF Mass Spectra of Phospholipids. in Zeitschrift fur Naturforschung. Section B: Journal of Chemical Sciences, 64(3), 331-334.
https://doi.org/10.1515/znb-2009-0314
https://hdl.handle.net/21.15107/rcub_vinar_3770

Petković M, Schiller J, Mueller M, Suess R, Arnold K, Arnhold J. Detection of Adducts with Matrix Clusters in the Positive and Negative Ion Mode MALDI-TOF Mass Spectra of Phospholipids. in Zeitschrift fur Naturforschung. Section B: Journal of Chemical Sciences. 2009;64(3):331-334.
doi:10.1515/znb-2009-0314
https://hdl.handle.net/21.15107/rcub_vinar_3770 .

Petković, Marijana, Schiller, Jürgen, Mueller, Matthias, Suess, Rosmarie, Arnold, Klaus, Arnhold, Juergen, "Detection of Adducts with Matrix Clusters in the Positive and Negative Ion Mode MALDI-TOF Mass Spectra of Phospholipids" in Zeitschrift fur Naturforschung. Section B: Journal of Chemical Sciences, 64, no. 3 (2009):331-334,
https://doi.org/10.1515/znb-2009-0314 .,
https://hdl.handle.net/21.15107/rcub_vinar_3770 .