TY  - CONF
AU  - Životić, Ivan
AU  - Mitrović, Kristina
AU  - Kolić, Ivana
AU  - Seke, Mariana
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12465
AB  - Introduction: Congenital anomalies of the kidney and urinary tracts(CAKUT) are a diverse spectrum of defects with complex etiology and not fully explained genetic background. miRNA-containing copy number variants (CNVs) are described as genetic risk factor for the disease development. We aimed to identify miRNAs with the maximum regulatory coverage of previously reported differentially expressed genes in CAKUT tissue compared to controls and bioinformatically characterize a set of these miRNAs which are located in common CNVs. Methods: Differentially expressed genes were identified from ureter tissue transcriptome open data GSE83946 from 15 CAKUT patients and 7 healthy controls, generated in house previously. miRPathDB v2.0 was used for identification of miRNAs with maximum coverage of DEGs(miRNAs which complimentarily regulate all DEGs). Mapping of maximum coverage miRNAs onto common CNVs (frequency >0.2) was performed using UCSC genome browser and gnomAD database. miRNA mapping common CNVs were further bioinformatically analyzed using miRPathDB v2.0. Results: In a maximum coverage set of 50 miRNAs interacting with DEGs in CAKUT, we have identified 3 miRNA geneslocated in the common CNVs(hsa-miR-663b, hsa-miR-3180-3p and hsa-miR-1302). Using Reactome database we identified all three miRNAsto be significantly enriched in the pathway Neuronal System: -log(p-value)>2.326 for hsa-miR-1302; -log(p-value)>1.556 for hsa-miR-3180-3p; and -log(pvalue)>1.703 for hsa-miR-663b. Conclusion: CAKUT is characterized with variable penetrability and expressivity and often followed with other comorbiditiessuch as neurodevelopmental disorders. miRNAsinvolved in DEG networks and prone to CNV effects could present modulating factors of the disease phenotype. Further studies should provide additional evidence about hsa-miR-1302, hsa-miR-3180-3p and hsa-miR-663b involvements in CAKUT etiology
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts
T1  - Identification of micro RNA from common copy number variants as risk factors for CAKUT
SP  - 62
EP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12465
ER  - 

@conference{
author = "Životić, Ivan and Mitrović, Kristina and Kolić, Ivana and Seke, Mariana and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan",
year = "2023",
abstract = "Introduction: Congenital anomalies of the kidney and urinary tracts(CAKUT) are a diverse spectrum of defects with complex etiology and not fully explained genetic background. miRNA-containing copy number variants (CNVs) are described as genetic risk factor for the disease development. We aimed to identify miRNAs with the maximum regulatory coverage of previously reported differentially expressed genes in CAKUT tissue compared to controls and bioinformatically characterize a set of these miRNAs which are located in common CNVs. Methods: Differentially expressed genes were identified from ureter tissue transcriptome open data GSE83946 from 15 CAKUT patients and 7 healthy controls, generated in house previously. miRPathDB v2.0 was used for identification of miRNAs with maximum coverage of DEGs(miRNAs which complimentarily regulate all DEGs). Mapping of maximum coverage miRNAs onto common CNVs (frequency >0.2) was performed using UCSC genome browser and gnomAD database. miRNA mapping common CNVs were further bioinformatically analyzed using miRPathDB v2.0. Results: In a maximum coverage set of 50 miRNAs interacting with DEGs in CAKUT, we have identified 3 miRNA geneslocated in the common CNVs(hsa-miR-663b, hsa-miR-3180-3p and hsa-miR-1302). Using Reactome database we identified all three miRNAsto be significantly enriched in the pathway Neuronal System: -log(p-value)>2.326 for hsa-miR-1302; -log(p-value)>1.556 for hsa-miR-3180-3p; and -log(pvalue)>1.703 for hsa-miR-663b. Conclusion: CAKUT is characterized with variable penetrability and expressivity and often followed with other comorbiditiessuch as neurodevelopmental disorders. miRNAsinvolved in DEG networks and prone to CNV effects could present modulating factors of the disease phenotype. Further studies should provide additional evidence about hsa-miR-1302, hsa-miR-3180-3p and hsa-miR-663b involvements in CAKUT etiology",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts",
title = "Identification of micro RNA from common copy number variants as risk factors for CAKUT",
pages = "62-62",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12465"
}

Životić, I., Mitrović, K., Kolić, I., Seke, M., Živković, M., Stanković, A.,& Jovanović, I.. (2023). Identification of micro RNA from common copy number variants as risk factors for CAKUT. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts, 62-62.
https://hdl.handle.net/21.15107/rcub_vinar_12465

Životić I, Mitrović K, Kolić I, Seke M, Živković M, Stanković A, Jovanović I. Identification of micro RNA from common copy number variants as risk factors for CAKUT. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts. 2023;:62-62.
https://hdl.handle.net/21.15107/rcub_vinar_12465 .

Životić, Ivan, Mitrović, Kristina, Kolić, Ivana, Seke, Mariana, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan, "Identification of micro RNA from common copy number variants as risk factors for CAKUT" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts (2023):62-62,
https://hdl.handle.net/21.15107/rcub_vinar_12465 .

TY  - CONF
AU  - Stojković, Ljiljana
AU  - Stefanović, Milan
AU  - Dinčić, Evica
AU  - Mačak, Nataša
AU  - Seke, Mariana
AU  - Živković, Maja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12712
AB  - Introduction: The hallmark pathogenic mechanisms of multiple sclerosis (MS) are proposed to be associated with long chain polyunsaturated fatty acids(LC-PUFA)-mediated neuroinflammation, through LC-PUFA-derived pro- and anti-inflammatory eicosanoids. Variants in genes coding for fatty acid desaturases (FADS), the key enzymes in LC-PUFA biosynthesis from essential fatty acids, are associated with changesin circulating LC-PUFA levels. The aim of thisstudy wasto investigate the FADS2 intronic variants, rs174576 (C/A), rs174593 (T/C) and rs174616 (G/A), in association with MS. Methods: The study involved 124 patients with relapsing-remitting form of MS and 83 healthy control subjects. The FADS2 gene variants were detected using TaqMan® SNP genotyping assays. Analysis of allele and genotype distributions in patients and controls was done by using the chi-square test. Results: According to the model of dominant effect of allele, genotypes containing the alternative, C, allele of FADS2 rs174593 variant were significantly less frequent in MS patients than in controls (MS: TT=57,26%, TC+CC=42,74%; controls: TT=42,17%, TC+CC=57,83%; p=0,03). In addition, the frequency of rs174593 C allele was significantly lower in patients, compared to controls (MS: T=0,76, C=0,24; controls: T=0,67, C=0,33; p=0,04). The frequency distributions of rs174576 and rs174616 alleles and genotypes were not significantly different between the study groups (p>0,05). Conclusion: The obtained resultssupply a rationale for further investigation of the association of FADS2 rs174593 with circulating LC-PUFA levels, in the context of MS. The genotype-LC-PUFA phenotype association could provide guidelinesfor personalized LC-PUFA supplementation, to potentially ameliorate the disease course and improve the effectiveness of therapy
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts
T1  - FADS2 gene variant rs174593 is associated with multiple sclerosis
SP  - 88
EP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12712
ER  - 

@conference{
author = "Stojković, Ljiljana and Stefanović, Milan and Dinčić, Evica and Mačak, Nataša and Seke, Mariana and Živković, Maja",
year = "2023",
abstract = "Introduction: The hallmark pathogenic mechanisms of multiple sclerosis (MS) are proposed to be associated with long chain polyunsaturated fatty acids(LC-PUFA)-mediated neuroinflammation, through LC-PUFA-derived pro- and anti-inflammatory eicosanoids. Variants in genes coding for fatty acid desaturases (FADS), the key enzymes in LC-PUFA biosynthesis from essential fatty acids, are associated with changesin circulating LC-PUFA levels. The aim of thisstudy wasto investigate the FADS2 intronic variants, rs174576 (C/A), rs174593 (T/C) and rs174616 (G/A), in association with MS. Methods: The study involved 124 patients with relapsing-remitting form of MS and 83 healthy control subjects. The FADS2 gene variants were detected using TaqMan® SNP genotyping assays. Analysis of allele and genotype distributions in patients and controls was done by using the chi-square test. Results: According to the model of dominant effect of allele, genotypes containing the alternative, C, allele of FADS2 rs174593 variant were significantly less frequent in MS patients than in controls (MS: TT=57,26%, TC+CC=42,74%; controls: TT=42,17%, TC+CC=57,83%; p=0,03). In addition, the frequency of rs174593 C allele was significantly lower in patients, compared to controls (MS: T=0,76, C=0,24; controls: T=0,67, C=0,33; p=0,04). The frequency distributions of rs174576 and rs174616 alleles and genotypes were not significantly different between the study groups (p>0,05). Conclusion: The obtained resultssupply a rationale for further investigation of the association of FADS2 rs174593 with circulating LC-PUFA levels, in the context of MS. The genotype-LC-PUFA phenotype association could provide guidelinesfor personalized LC-PUFA supplementation, to potentially ameliorate the disease course and improve the effectiveness of therapy",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts",
title = "FADS2 gene variant rs174593 is associated with multiple sclerosis",
pages = "88-88",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12712"
}

Stojković, L., Stefanović, M., Dinčić, E., Mačak, N., Seke, M.,& Živković, M.. (2023). FADS2 gene variant rs174593 is associated with multiple sclerosis. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts, 88-88.
https://hdl.handle.net/21.15107/rcub_vinar_12712

Stojković L, Stefanović M, Dinčić E, Mačak N, Seke M, Živković M. FADS2 gene variant rs174593 is associated with multiple sclerosis. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts. 2023;:88-88.
https://hdl.handle.net/21.15107/rcub_vinar_12712 .

Stojković, Ljiljana, Stefanović, Milan, Dinčić, Evica, Mačak, Nataša, Seke, Mariana, Živković, Maja, "FADS2 gene variant rs174593 is associated with multiple sclerosis" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts (2023):88-88,
https://hdl.handle.net/21.15107/rcub_vinar_12712 .

TY  - JOUR
AU  - Jović, Danica
AU  - Jaćević, Vesna
AU  - Kuča, Kamil
AU  - Borišev, Ivana
AU  - Mrđanović, Jasminka
AU  - Petrović, Danijela
AU  - Seke, Mariana
AU  - Đorđević, Aleksandar
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9120
AB  - Being a member of the nanofamily, carbon nanomaterials exhibit specific properties that mostly arise from their small size. They have proved to be very promising for application in the technical and biomedical field. A wide spectrum of use implies the inevitable presence of carbon nanomaterials in the environment, thus potentially endangering their whole nature. Although scientists worldwide have conducted research investigating the impact of these materials, it is evident that there are still significant gaps concerning the knowledge of their mechanisms, as well as the prolonged and chronic exposure and effects. This manuscript summarizes the most prominent representatives of carbon nanomaterial groups, giving a brief review of their general physico-chemical properties, the most common use, and toxicity profiles. Toxicity was presented through genotoxicity and the activation of the cell signaling pathways, both including in vitro and in vivo models, mechanisms, and the consequential outcomes. Moreover, the acute toxicity of fullerenol, as one of the most commonly investigated members, was briefly presented in the final part of this review. Thinking small can greatly help us improve our lives, but also obliges us to deeply and comprehensively investigate all the possible consequences that could arise from our pure-hearted scientific ambitions and work.
T2  - Nanomaterials
T1  - The Puzzling Potential of Carbon Nanomaterials: General Properties, Application, and Toxicity
VL  - 10
IS  - 8
SP  - 1508
DO  - 10.3390/nano10081508
ER  - 

@article{
author = "Jović, Danica and Jaćević, Vesna and Kuča, Kamil and Borišev, Ivana and Mrđanović, Jasminka and Petrović, Danijela and Seke, Mariana and Đorđević, Aleksandar",
year = "2020",
abstract = "Being a member of the nanofamily, carbon nanomaterials exhibit specific properties that mostly arise from their small size. They have proved to be very promising for application in the technical and biomedical field. A wide spectrum of use implies the inevitable presence of carbon nanomaterials in the environment, thus potentially endangering their whole nature. Although scientists worldwide have conducted research investigating the impact of these materials, it is evident that there are still significant gaps concerning the knowledge of their mechanisms, as well as the prolonged and chronic exposure and effects. This manuscript summarizes the most prominent representatives of carbon nanomaterial groups, giving a brief review of their general physico-chemical properties, the most common use, and toxicity profiles. Toxicity was presented through genotoxicity and the activation of the cell signaling pathways, both including in vitro and in vivo models, mechanisms, and the consequential outcomes. Moreover, the acute toxicity of fullerenol, as one of the most commonly investigated members, was briefly presented in the final part of this review. Thinking small can greatly help us improve our lives, but also obliges us to deeply and comprehensively investigate all the possible consequences that could arise from our pure-hearted scientific ambitions and work.",
journal = "Nanomaterials",
title = "The Puzzling Potential of Carbon Nanomaterials: General Properties, Application, and Toxicity",
volume = "10",
number = "8",
pages = "1508",
doi = "10.3390/nano10081508"
}

Jović, D., Jaćević, V., Kuča, K., Borišev, I., Mrđanović, J., Petrović, D., Seke, M.,& Đorđević, A.. (2020). The Puzzling Potential of Carbon Nanomaterials: General Properties, Application, and Toxicity. in Nanomaterials, 10(8), 1508.
https://doi.org/10.3390/nano10081508

Jović D, Jaćević V, Kuča K, Borišev I, Mrđanović J, Petrović D, Seke M, Đorđević A. The Puzzling Potential of Carbon Nanomaterials: General Properties, Application, and Toxicity. in Nanomaterials. 2020;10(8):1508.
doi:10.3390/nano10081508 .

Jović, Danica, Jaćević, Vesna, Kuča, Kamil, Borišev, Ivana, Mrđanović, Jasminka, Petrović, Danijela, Seke, Mariana, Đorđević, Aleksandar, "The Puzzling Potential of Carbon Nanomaterials: General Properties, Application, and Toxicity" in Nanomaterials, 10, no. 8 (2020):1508,
https://doi.org/10.3390/nano10081508 . .

TY  - JOUR
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Labudović-Borović, Milica
AU  - Borisev, Ivana
AU  - Novaković, Mirjana M.
AU  - Rakočević, Zlatko Lj.
AU  - Đorđević, Aleksandar N.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8649
AB  - Fullerenol C60(OH)24 with its spherical shape, symmetrical structure, 1 nm size and the ability to form polyionic nanoparticles in water solution, was used to synthesise a novel nanocomposite made of fullerenol nanoparticles (FNP) and iron ions (Fe2+). The FNP/Fe2+ nanocomposite was characterised by DLS and TEM-EDS analyses which have shown that the size distribution of FNP/Fe2+ stayed in the same scope as the size distribution of FNP, ranging from 11 to 60 nm. However, Fe2+ did affect the change of FNP’s zeta potential (− 49.2 mV), shifting it to more positive values (− 30.8 mV). In this study, it was assumed that FNP/Fe2+ could reduce the toxic effects of doxorubicin (Dox). Male Wistar rats were treated i.p. with FNP/Fe2+ nanocomposite 1 h prior to Dox treatment. At the subcellular level, the ultrastructural analysis revealed minor alterations sporadically displayed within the heart and liver tissues. Moreover, at the molecular level, the gene expressions analysis of mRNAs for catalase (heart and liver) and MnSOD (only liver) were significantly downregulated, indicating reduction in oxidative stress. Overall, the pretreatment with FNP/Fe2+ nanocomposite, followed by Dox application, significantly diminished harmful effects of the applied drug on the heart and liver, suggesting the potential protective effect of the nanocomposite on the healthy tissues. © 2019, Springer Nature B.V.
T2  - Journal of Nanoparticle Research
T1  - Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity
VL  - 21
IS  - 11
SP  - 239
DO  - 10.1007/s11051-019-4681-4
ER  - 

@article{
author = "Seke, Mariana and Petrović, Danijela and Labudović-Borović, Milica and Borisev, Ivana and Novaković, Mirjana M. and Rakočević, Zlatko Lj. and Đorđević, Aleksandar N.",
year = "2019",
abstract = "Fullerenol C60(OH)24 with its spherical shape, symmetrical structure, 1 nm size and the ability to form polyionic nanoparticles in water solution, was used to synthesise a novel nanocomposite made of fullerenol nanoparticles (FNP) and iron ions (Fe2+). The FNP/Fe2+ nanocomposite was characterised by DLS and TEM-EDS analyses which have shown that the size distribution of FNP/Fe2+ stayed in the same scope as the size distribution of FNP, ranging from 11 to 60 nm. However, Fe2+ did affect the change of FNP’s zeta potential (− 49.2 mV), shifting it to more positive values (− 30.8 mV). In this study, it was assumed that FNP/Fe2+ could reduce the toxic effects of doxorubicin (Dox). Male Wistar rats were treated i.p. with FNP/Fe2+ nanocomposite 1 h prior to Dox treatment. At the subcellular level, the ultrastructural analysis revealed minor alterations sporadically displayed within the heart and liver tissues. Moreover, at the molecular level, the gene expressions analysis of mRNAs for catalase (heart and liver) and MnSOD (only liver) were significantly downregulated, indicating reduction in oxidative stress. Overall, the pretreatment with FNP/Fe2+ nanocomposite, followed by Dox application, significantly diminished harmful effects of the applied drug on the heart and liver, suggesting the potential protective effect of the nanocomposite on the healthy tissues. © 2019, Springer Nature B.V.",
journal = "Journal of Nanoparticle Research",
title = "Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity",
volume = "21",
number = "11",
pages = "239",
doi = "10.1007/s11051-019-4681-4"
}

Seke, M., Petrović, D., Labudović-Borović, M., Borisev, I., Novaković, M. M., Rakočević, Z. Lj.,& Đorđević, A. N.. (2019). Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity. in Journal of Nanoparticle Research, 21(11), 239.
https://doi.org/10.1007/s11051-019-4681-4

Seke M, Petrović D, Labudović-Borović M, Borisev I, Novaković MM, Rakočević ZL, Đorđević AN. Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity. in Journal of Nanoparticle Research. 2019;21(11):239.
doi:10.1007/s11051-019-4681-4 .

Seke, Mariana, Petrović, Danijela, Labudović-Borović, Milica, Borisev, Ivana, Novaković, Mirjana M., Rakočević, Zlatko Lj., Đorđević, Aleksandar N., "Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity" in Journal of Nanoparticle Research, 21, no. 11 (2019):239,
https://doi.org/10.1007/s11051-019-4681-4 . .

TY  - JOUR
AU  - Petrović, Danijela
AU  - Seke, Mariana
AU  - Labudović-Borović, Milica
AU  - Jović, Danica S.
AU  - Borišev, Ivana
AU  - Srđenović, Branislava U.
AU  - Rakočević, Zlatko Lj.
AU  - Pavlović, Vladimir B.
AU  - Đorđević, Aleksandar N.
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0014480017305890
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7688
AB  - In our recent studies we have designed fullerenol/doxorubicin nanocomposite (FNP/DOX) as the new drug nanocarrier. This research has demonstrated that this novel nanocomposite has had better implications on the liver tissue in vivo (Wistar rats treated intraperitoneally), than treatment based only on DOX. FNP/DOX has been characterised by DLS, TEM and AFM measurements which have shown that DOX loaded onto FNP did not influence fullerenol nanoparticle's size. FNP/DOX affected oxidative status in blood causing a significant decrease of catalase and SOD activity in comparison to DOX, implicating the reduction in oxidative stress. qRT-PCR results on the mRNA level of antioxidative enzymes (catalase and MnSOD) revealed that the effect of oxidative stress is significantly reduced by the treatment with FNP/DOX (p <.05). The ultrastructural analysis of the liver tissue has revealed that FNP/DOX nanocomposite generated considerably less damage in the liver tissue, than DOX applied at the same dose. Hence, our results have indicated that FNP, within FNP/DOX nanocomposite, exhibits protective effects to the liver tissue of the healthy rats.
T2  - Experimental and Molecular Pathology
T1  - Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats
VL  - 104
IS  - 3
SP  - 199
EP  - 211
DO  - 10.1016/j.yexmp.2018.04.005
ER  - 

@article{
author = "Petrović, Danijela and Seke, Mariana and Labudović-Borović, Milica and Jović, Danica S. and Borišev, Ivana and Srđenović, Branislava U. and Rakočević, Zlatko Lj. and Pavlović, Vladimir B. and Đorđević, Aleksandar N.",
year = "2018",
abstract = "In our recent studies we have designed fullerenol/doxorubicin nanocomposite (FNP/DOX) as the new drug nanocarrier. This research has demonstrated that this novel nanocomposite has had better implications on the liver tissue in vivo (Wistar rats treated intraperitoneally), than treatment based only on DOX. FNP/DOX has been characterised by DLS, TEM and AFM measurements which have shown that DOX loaded onto FNP did not influence fullerenol nanoparticle's size. FNP/DOX affected oxidative status in blood causing a significant decrease of catalase and SOD activity in comparison to DOX, implicating the reduction in oxidative stress. qRT-PCR results on the mRNA level of antioxidative enzymes (catalase and MnSOD) revealed that the effect of oxidative stress is significantly reduced by the treatment with FNP/DOX (p <.05). The ultrastructural analysis of the liver tissue has revealed that FNP/DOX nanocomposite generated considerably less damage in the liver tissue, than DOX applied at the same dose. Hence, our results have indicated that FNP, within FNP/DOX nanocomposite, exhibits protective effects to the liver tissue of the healthy rats.",
journal = "Experimental and Molecular Pathology",
title = "Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats",
volume = "104",
number = "3",
pages = "199-211",
doi = "10.1016/j.yexmp.2018.04.005"
}

Petrović, D., Seke, M., Labudović-Borović, M., Jović, D. S., Borišev, I., Srđenović, B. U., Rakočević, Z. Lj., Pavlović, V. B.,& Đorđević, A. N.. (2018). Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats. in Experimental and Molecular Pathology, 104(3), 199-211.
https://doi.org/10.1016/j.yexmp.2018.04.005

Petrović D, Seke M, Labudović-Borović M, Jović DS, Borišev I, Srđenović BU, Rakočević ZL, Pavlović VB, Đorđević AN. Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats. in Experimental and Molecular Pathology. 2018;104(3):199-211.
doi:10.1016/j.yexmp.2018.04.005 .

Petrović, Danijela, Seke, Mariana, Labudović-Borović, Milica, Jović, Danica S., Borišev, Ivana, Srđenović, Branislava U., Rakočević, Zlatko Lj., Pavlović, Vladimir B., Đorđević, Aleksandar N., "Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats" in Experimental and Molecular Pathology, 104, no. 3 (2018):199-211,
https://doi.org/10.1016/j.yexmp.2018.04.005 . .

TY  - JOUR
AU  - Borišev, Ivana
AU  - Mrđanović, Jasminka Ž.
AU  - Petrović, Danijela
AU  - Seke, Mariana
AU  - Jović, Danica S.
AU  - Srđenović, Branislava U.
AU  - Latinović, Nataša
AU  - Đorđević, Aleksandar N.
PY  - 2018
UR  - http://stacks.iop.org/0957-4484/29/i=33/a=332002?key=crossref.4804877570e2609bf6333877ee495ab3
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7751
AB  - Nanotechnology, focused on discovery and development of new pharmaceutical products is known as nanopharmacology, and one research area this branch is engaged in are nanopharmaceuticals. The importance of being nano has been particularly emphasized in scientific areas dealing with nanomedicine and nanopharmaceuticals. Nanopharmaceuticals, their routes of administration, obstacles and solutions concerning their improved application and enhanced efficacy have been briefly yet comprehensively described. Cancer is one of the leading causes of death worldwide and evergrowing number of scientific research on the topic only confirms that the needs have not been completed yet and that there is a wide platform for improvement. This is undoubtedly true for nanoformulations of an anticancer drug doxorubicin, where various nanocarrriers were given an important role to reduce the drug toxicity, while the efficacy of the drug was supposed to be retained or preferably enhanced. Therefore, we present an interdisciplinary comprehensive overview of interdisciplinary nature on nanopharmaceuticals based on doxorubicin and its nanoformulations with valuable information concerning trends, obstacles and prospective of nanopharmaceuticals development, mode of activity of sole drug doxorubicin and its nanoformulations based on different nanocarriers, their brief descriptions of biological activity through assessing in vitro and in vivo behavior.
T2  - Nanotechnology
T1  - Nanoformulations of doxorubicin: How far have we come and where do we go from here?
VL  - 29
IS  - 33
SP  - 332002
DO  - 10.1088/1361-6528/aac7dd
ER  - 

@article{
author = "Borišev, Ivana and Mrđanović, Jasminka Ž. and Petrović, Danijela and Seke, Mariana and Jović, Danica S. and Srđenović, Branislava U. and Latinović, Nataša and Đorđević, Aleksandar N.",
year = "2018",
abstract = "Nanotechnology, focused on discovery and development of new pharmaceutical products is known as nanopharmacology, and one research area this branch is engaged in are nanopharmaceuticals. The importance of being nano has been particularly emphasized in scientific areas dealing with nanomedicine and nanopharmaceuticals. Nanopharmaceuticals, their routes of administration, obstacles and solutions concerning their improved application and enhanced efficacy have been briefly yet comprehensively described. Cancer is one of the leading causes of death worldwide and evergrowing number of scientific research on the topic only confirms that the needs have not been completed yet and that there is a wide platform for improvement. This is undoubtedly true for nanoformulations of an anticancer drug doxorubicin, where various nanocarrriers were given an important role to reduce the drug toxicity, while the efficacy of the drug was supposed to be retained or preferably enhanced. Therefore, we present an interdisciplinary comprehensive overview of interdisciplinary nature on nanopharmaceuticals based on doxorubicin and its nanoformulations with valuable information concerning trends, obstacles and prospective of nanopharmaceuticals development, mode of activity of sole drug doxorubicin and its nanoformulations based on different nanocarriers, their brief descriptions of biological activity through assessing in vitro and in vivo behavior.",
journal = "Nanotechnology",
title = "Nanoformulations of doxorubicin: How far have we come and where do we go from here?",
volume = "29",
number = "33",
pages = "332002",
doi = "10.1088/1361-6528/aac7dd"
}

Borišev, I., Mrđanović, J. Ž., Petrović, D., Seke, M., Jović, D. S., Srđenović, B. U., Latinović, N.,& Đorđević, A. N.. (2018). Nanoformulations of doxorubicin: How far have we come and where do we go from here?. in Nanotechnology, 29(33), 332002.
https://doi.org/10.1088/1361-6528/aac7dd

Borišev I, Mrđanović JŽ, Petrović D, Seke M, Jović DS, Srđenović BU, Latinović N, Đorđević AN. Nanoformulations of doxorubicin: How far have we come and where do we go from here?. in Nanotechnology. 2018;29(33):332002.
doi:10.1088/1361-6528/aac7dd .

Borišev, Ivana, Mrđanović, Jasminka Ž., Petrović, Danijela, Seke, Mariana, Jović, Danica S., Srđenović, Branislava U., Latinović, Nataša, Đorđević, Aleksandar N., "Nanoformulations of doxorubicin: How far have we come and where do we go from here?" in Nanotechnology, 29, no. 33 (2018):332002,
https://doi.org/10.1088/1361-6528/aac7dd . .

TY  - CONF
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Labudović-Borović, Milica
AU  - Jović, Danica S.
AU  - Borišev, Ivana
AU  - Kanacki, Zdenko
AU  - Zikić, Dragan
AU  - Đorđević, Aleksandar N.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7182
AB  - Background: Doxorubicin is a first line cancer chemotherapeutic. Unfortunately, its clinical use is limited by its cardiotoxicity. It is known that iron overload aggravates anthracycline toxicity. Fullerenol is a 1 nm size molecule and in aqueous solutions is in the form of polyanionic nanoparticles, which enables them to serve as a good carrier of positively charged ions such as Fe2+. Fullerenol’s antioxidant activity through scavenging free radicals has already been proved in different biological systems.
Methods: The aim of our study was to investigate the effects of the fullerenol/iron nanocomposite as a pretreatment to doxorubicin on the rat’s heart in comparison to doxorubicin alone. After the 24h-treatment, adult male Wistar rats were sacrificed and hearts were collected for ultrastructural and qRT-PCR analysis. Considering the ability of doxorubicin to induce oxidative stress, and the fullerenol’s capability to mitigate it, we had chosen to monitor gene expression of enzymes involved in antioxidant defense.
Results: Ultrastructural study revealed that in the group pretreated with the nanocomposite prior to doxorubicin application cardiomyocytes were with preserved morphology and the structure of intercalated discs. On the other hand, the heart tissues of animals treated with doxorubicin alone were significantly more damaged. Intensive interstitial edema was observed, as well as vacuolization of cardiomyocytes, hypercontraction of sarcomeres, mitochondria of irregular shapes. qRT-PCR results have shown that neither treatment with doxorubicin alone nor the pretreatment with the nanocomposite did cause significant increase in mRNA levels of catalase and superoxide dismutase.
Conclusions: Our results indicate that the fullerenol/iron nanocomposite applied as pretreatment to doxorubicin induces less damage to the hearth tissue in comparison to doxorubicin alone.
C3  - Annals of Oncology
T1  - Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity
VL  - 28
IS  - Supplement 5
DO  - 10.1093/annonc/mdx390.057
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7182
ER  - 

@conference{
author = "Seke, Mariana and Petrović, Danijela and Labudović-Borović, Milica and Jović, Danica S. and Borišev, Ivana and Kanacki, Zdenko and Zikić, Dragan and Đorđević, Aleksandar N.",
year = "2017",
abstract = "Background: Doxorubicin is a first line cancer chemotherapeutic. Unfortunately, its clinical use is limited by its cardiotoxicity. It is known that iron overload aggravates anthracycline toxicity. Fullerenol is a 1 nm size molecule and in aqueous solutions is in the form of polyanionic nanoparticles, which enables them to serve as a good carrier of positively charged ions such as Fe2+. Fullerenol’s antioxidant activity through scavenging free radicals has already been proved in different biological systems.
Methods: The aim of our study was to investigate the effects of the fullerenol/iron nanocomposite as a pretreatment to doxorubicin on the rat’s heart in comparison to doxorubicin alone. After the 24h-treatment, adult male Wistar rats were sacrificed and hearts were collected for ultrastructural and qRT-PCR analysis. Considering the ability of doxorubicin to induce oxidative stress, and the fullerenol’s capability to mitigate it, we had chosen to monitor gene expression of enzymes involved in antioxidant defense.
Results: Ultrastructural study revealed that in the group pretreated with the nanocomposite prior to doxorubicin application cardiomyocytes were with preserved morphology and the structure of intercalated discs. On the other hand, the heart tissues of animals treated with doxorubicin alone were significantly more damaged. Intensive interstitial edema was observed, as well as vacuolization of cardiomyocytes, hypercontraction of sarcomeres, mitochondria of irregular shapes. qRT-PCR results have shown that neither treatment with doxorubicin alone nor the pretreatment with the nanocomposite did cause significant increase in mRNA levels of catalase and superoxide dismutase.
Conclusions: Our results indicate that the fullerenol/iron nanocomposite applied as pretreatment to doxorubicin induces less damage to the hearth tissue in comparison to doxorubicin alone.",
journal = "Annals of Oncology",
title = "Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity",
volume = "28",
number = "Supplement 5",
doi = "10.1093/annonc/mdx390.057",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7182"
}

Seke, M., Petrović, D., Labudović-Borović, M., Jović, D. S., Borišev, I., Kanacki, Z., Zikić, D.,& Đorđević, A. N.. (2017). Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity. in Annals of Oncology, 28(Supplement 5).
https://doi.org/10.1093/annonc/mdx390.057
https://hdl.handle.net/21.15107/rcub_vinar_7182

Seke M, Petrović D, Labudović-Borović M, Jović DS, Borišev I, Kanacki Z, Zikić D, Đorđević AN. Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity. in Annals of Oncology. 2017;28(Supplement 5).
doi:10.1093/annonc/mdx390.057
https://hdl.handle.net/21.15107/rcub_vinar_7182 .

Seke, Mariana, Petrović, Danijela, Labudović-Borović, Milica, Jović, Danica S., Borišev, Ivana, Kanacki, Zdenko, Zikić, Dragan, Đorđević, Aleksandar N., "Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity" in Annals of Oncology, 28, no. Supplement 5 (2017),
https://doi.org/10.1093/annonc/mdx390.057 .,
https://hdl.handle.net/21.15107/rcub_vinar_7182 .

TY  - JOUR
AU  - Seke, Mariana
AU  - Markelić, Milica
AU  - Morina, Arian
AU  - Jović, Danica S.
AU  - Korać, Aleksandra
AU  - Miličić, Dragana
AU  - Đorđević, Aleksandar N.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1609
AB  - Adsorption of non-polar compounds by suspended fullerene nanoaggregates (nC(60)) may enhance their toxicity and affect the fate, transformation, and transport of non-polar compounds in the environment. The potential of stable fullerene nanoaggregates as contaminant carriers in aqueous systems and the influence of chloromethanes (trichloromethane and dichloromethane) were studied on the midgut epithelial cells of Daphnia magna by light and electron microscopy. The size and shape of fullerene nanoaggregates were observed and measured using dynamic light scattering, transmission electron microscopy, and low vacuum scanning electron microscopy. The nC(60) in suspension appeared as a bulk of aggregates of irregular shape with a surface consisting of small clumps 20-30 nm in diameter. The presence of nC(60) aggregates was confirmed in midgut lumen and epithelial cells of D. magna. After in vivo acute exposure to chloromethane, light and electron microscopy revealed an extensive cytoplasmic vacuolization with disruption and loss of specific structures of D. magna midgut epithelium (mitochondria, endoplasmic reticulum, microvilli, peritrophic membrane) and increased appearance of necrotic cells. The degree of observed changes depended on the type of treatment: trichloromethane (TCM) induced the most notable changes, whereas fullerene nanoaggregates alone had no negative effects. Transmission electron microscopy also indicated increased lysosomal degradation and severe peroxidative damages of enterocyte mitochondria following combined exposure to chloromethane and fullerene nanoaggregates. In conclusion, the adsorption of chloromethane by fullerene nanoaggregates enhances their toxicity and induces peroxidative mitochondrial damage in midgut enterocytes.
T2  - Protoplasma
T1  - Synergistic mitotoxicity of chloromethanes and fullerene C-60 nanoaggregates in Daphnia magna midgut epithelial cells
VL  - 254
IS  - 4
SP  - 1607
EP  - 1616
DO  - 10.1007/s00709-016-1049-9
ER  - 

@article{
author = "Seke, Mariana and Markelić, Milica and Morina, Arian and Jović, Danica S. and Korać, Aleksandra and Miličić, Dragana and Đorđević, Aleksandar N.",
year = "2017",
abstract = "Adsorption of non-polar compounds by suspended fullerene nanoaggregates (nC(60)) may enhance their toxicity and affect the fate, transformation, and transport of non-polar compounds in the environment. The potential of stable fullerene nanoaggregates as contaminant carriers in aqueous systems and the influence of chloromethanes (trichloromethane and dichloromethane) were studied on the midgut epithelial cells of Daphnia magna by light and electron microscopy. The size and shape of fullerene nanoaggregates were observed and measured using dynamic light scattering, transmission electron microscopy, and low vacuum scanning electron microscopy. The nC(60) in suspension appeared as a bulk of aggregates of irregular shape with a surface consisting of small clumps 20-30 nm in diameter. The presence of nC(60) aggregates was confirmed in midgut lumen and epithelial cells of D. magna. After in vivo acute exposure to chloromethane, light and electron microscopy revealed an extensive cytoplasmic vacuolization with disruption and loss of specific structures of D. magna midgut epithelium (mitochondria, endoplasmic reticulum, microvilli, peritrophic membrane) and increased appearance of necrotic cells. The degree of observed changes depended on the type of treatment: trichloromethane (TCM) induced the most notable changes, whereas fullerene nanoaggregates alone had no negative effects. Transmission electron microscopy also indicated increased lysosomal degradation and severe peroxidative damages of enterocyte mitochondria following combined exposure to chloromethane and fullerene nanoaggregates. In conclusion, the adsorption of chloromethane by fullerene nanoaggregates enhances their toxicity and induces peroxidative mitochondrial damage in midgut enterocytes.",
journal = "Protoplasma",
title = "Synergistic mitotoxicity of chloromethanes and fullerene C-60 nanoaggregates in Daphnia magna midgut epithelial cells",
volume = "254",
number = "4",
pages = "1607-1616",
doi = "10.1007/s00709-016-1049-9"
}

Seke, M., Markelić, M., Morina, A., Jović, D. S., Korać, A., Miličić, D.,& Đorđević, A. N.. (2017). Synergistic mitotoxicity of chloromethanes and fullerene C-60 nanoaggregates in Daphnia magna midgut epithelial cells. in Protoplasma, 254(4), 1607-1616.
https://doi.org/10.1007/s00709-016-1049-9

Seke M, Markelić M, Morina A, Jović DS, Korać A, Miličić D, Đorđević AN. Synergistic mitotoxicity of chloromethanes and fullerene C-60 nanoaggregates in Daphnia magna midgut epithelial cells. in Protoplasma. 2017;254(4):1607-1616.
doi:10.1007/s00709-016-1049-9 .

Seke, Mariana, Markelić, Milica, Morina, Arian, Jović, Danica S., Korać, Aleksandra, Miličić, Dragana, Đorđević, Aleksandar N., "Synergistic mitotoxicity of chloromethanes and fullerene C-60 nanoaggregates in Daphnia magna midgut epithelial cells" in Protoplasma, 254, no. 4 (2017):1607-1616,
https://doi.org/10.1007/s00709-016-1049-9 . .

TY  - CONF
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Labudović Borović, Milica
AU  - Jović, Danica
AU  - Borišev, Ivana
AU  - Kanački, Zdenko
AU  - Žikić, Dragan
AU  - Đorđević, Aleksandar
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12680
AB  - Introduction: Doxorubicin is the most prominent chemotherapeutic, but its clinical use is limited by its severe systemic toxicity. An iron overload aggravates anthracycline toxicity. Fullerenol in aqueous solutions is in the form of polyanionic nanoparticles, serving as a good carrier of positively charged ions, such as Fe2+. Fullerenol’s antioxidant activity has already been proved in different biological systems. The aim of our study was to investigate the effects of the fullerenol/iron nanocomposite on the rat liver as a pretreatment to doxorubicin application. Methods: After the 24h-treatment, adult male Wistar rats were sacrificed and livers were collected for ultrastructural and qRT-PCR analysis. Considering the ability of doxorubicin to induce oxidative stress, and the fullerenol’s capability to mitigate it, gene expression of enzymes involved in antioxidant defense was measured. Results: Ultrastructural analysis revealed that liver tissue was mainly preserved after the nanocomposite was applied prior to doxorubicin. However, the hepatocytes of animals treated with doxorubicin, presented significantly damaged morphology. Apoptosis of hepatocytes and endothelial cells, mitochondria of irregular size and with disruption of cristae, diffuse injury of capillaries were observed. RT-PCR results have shown that treatment with doxorubicin alone significantly increase the mRNA levels of catalase (p=0.008) and superoxide-dismutase (p=0.000003), while the pretreatment with the nanocomposite prior the doxirubicine treatment, dramaticly downregulated the mRNA levels of catalase (p=0.0004) and superoxide-dismutase (p=0.0001). Conclusion: Our results suggest that the fullerenol/iron nanocomposite applied as pretreatment to doxorubicin, demonstrated protection to the liver tissue and induced less damage to the hepatocytes in comparison to doxorubicin alone.
PB  - Belgrade : University of Belgrade, Faculty of Biology
C3  - CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
T1  - Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity
SP  - 71
EP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12680
ER  - 

@conference{
author = "Seke, Mariana and Petrović, Danijela and Labudović Borović, Milica and Jović, Danica and Borišev, Ivana and Kanački, Zdenko and Žikić, Dragan and Đorđević, Aleksandar",
year = "2017",
abstract = "Introduction: Doxorubicin is the most prominent chemotherapeutic, but its clinical use is limited by its severe systemic toxicity. An iron overload aggravates anthracycline toxicity. Fullerenol in aqueous solutions is in the form of polyanionic nanoparticles, serving as a good carrier of positively charged ions, such as Fe2+. Fullerenol’s antioxidant activity has already been proved in different biological systems. The aim of our study was to investigate the effects of the fullerenol/iron nanocomposite on the rat liver as a pretreatment to doxorubicin application. Methods: After the 24h-treatment, adult male Wistar rats were sacrificed and livers were collected for ultrastructural and qRT-PCR analysis. Considering the ability of doxorubicin to induce oxidative stress, and the fullerenol’s capability to mitigate it, gene expression of enzymes involved in antioxidant defense was measured. Results: Ultrastructural analysis revealed that liver tissue was mainly preserved after the nanocomposite was applied prior to doxorubicin. However, the hepatocytes of animals treated with doxorubicin, presented significantly damaged morphology. Apoptosis of hepatocytes and endothelial cells, mitochondria of irregular size and with disruption of cristae, diffuse injury of capillaries were observed. RT-PCR results have shown that treatment with doxorubicin alone significantly increase the mRNA levels of catalase (p=0.008) and superoxide-dismutase (p=0.000003), while the pretreatment with the nanocomposite prior the doxirubicine treatment, dramaticly downregulated the mRNA levels of catalase (p=0.0004) and superoxide-dismutase (p=0.0001). Conclusion: Our results suggest that the fullerenol/iron nanocomposite applied as pretreatment to doxorubicin, demonstrated protection to the liver tissue and induced less damage to the hepatocytes in comparison to doxorubicin alone.",
publisher = "Belgrade : University of Belgrade, Faculty of Biology",
journal = "CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts",
title = "Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity",
pages = "71-71",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12680"
}

Seke, M., Petrović, D., Labudović Borović, M., Jović, D., Borišev, I., Kanački, Z., Žikić, D.,& Đorđević, A.. (2017). Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
Belgrade : University of Belgrade, Faculty of Biology., 71-71.
https://hdl.handle.net/21.15107/rcub_vinar_12680

Seke M, Petrović D, Labudović Borović M, Jović D, Borišev I, Kanački Z, Žikić D, Đorđević A. Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts. 2017;:71-71.
https://hdl.handle.net/21.15107/rcub_vinar_12680 .

Seke, Mariana, Petrović, Danijela, Labudović Borović, Milica, Jović, Danica, Borišev, Ivana, Kanački, Zdenko, Žikić, Dragan, Đorđević, Aleksandar, "Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity" in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts (2017):71-71,
https://hdl.handle.net/21.15107/rcub_vinar_12680 .

TY  - JOUR
AU  - Jović, Danica S.
AU  - Seke, Mariana
AU  - Đorđević, Aleksandar N.
AU  - Mrđanović, Jasminka Ž.
AU  - Aleksić, Lidija D.
AU  - Bogdanović, Gordana M.
AU  - Pavić, Aleksandar B.
AU  - Plavec, Janez
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1042
AB  - Doxorubicin is a very potent chemotherapeutic drug, however its side effects limit its clinical use. The aim of this research was to investigate the properties of a fullerenol/doxorubicin nanocomposite, its potentially cytotoxic and genotoxic effects on malignant cell lines, as well as its toxicity towards zebra fish embryos. Chromatographic, NMR and mass spectral analysis of the nanocomposite imply that interactions between doxorubicin and fullerenol are non-covalent bonds. The stability of the nanocomposite was confirmed by the use of atomic force microscopy, dynamic light scattering and transmission electron microscopy. The nanocomposite, compared to the free doxorubicin at equivalent concentrations, significantly decreased the viability of MCF-7 and MDA-MB-231 cells. The flow cytometry results indicated that doxorubicin-loaded fullerenol could remarkably increase the uptake of doxorubicin suggesting that fullerenol might be a promising intracellular targeting carrier for the efficient delivery of antitumor drugs into tumor cells. The nanocomposite also affected cell cycle distribution. A genotoxicity test showed that the nanocomposite at all examined concentrations on MCF-7 and at lower concentrations on MDA-MB-231 cells caused DNA damage. Consequently, cell proliferation was notably reduced when compared with controls. Results of the zebrafish embryotoxicity assay showed a decreased overall toxicity, particularly cardiotoxicity and increased safety of the nanocomposite in comparison to doxorubicin alone, as manifested by a higher survival of embryos and less pericardial edema.
T2  - RSC Advances
T1  - Fullerenol nanoparticles as a new delivery system for doxorubicin
VL  - 6
IS  - 45
SP  - 38563
EP  - 38578
DO  - 10.1039/c6ra03879d
ER  - 

@article{
author = "Jović, Danica S. and Seke, Mariana and Đorđević, Aleksandar N. and Mrđanović, Jasminka Ž. and Aleksić, Lidija D. and Bogdanović, Gordana M. and Pavić, Aleksandar B. and Plavec, Janez",
year = "2016",
abstract = "Doxorubicin is a very potent chemotherapeutic drug, however its side effects limit its clinical use. The aim of this research was to investigate the properties of a fullerenol/doxorubicin nanocomposite, its potentially cytotoxic and genotoxic effects on malignant cell lines, as well as its toxicity towards zebra fish embryos. Chromatographic, NMR and mass spectral analysis of the nanocomposite imply that interactions between doxorubicin and fullerenol are non-covalent bonds. The stability of the nanocomposite was confirmed by the use of atomic force microscopy, dynamic light scattering and transmission electron microscopy. The nanocomposite, compared to the free doxorubicin at equivalent concentrations, significantly decreased the viability of MCF-7 and MDA-MB-231 cells. The flow cytometry results indicated that doxorubicin-loaded fullerenol could remarkably increase the uptake of doxorubicin suggesting that fullerenol might be a promising intracellular targeting carrier for the efficient delivery of antitumor drugs into tumor cells. The nanocomposite also affected cell cycle distribution. A genotoxicity test showed that the nanocomposite at all examined concentrations on MCF-7 and at lower concentrations on MDA-MB-231 cells caused DNA damage. Consequently, cell proliferation was notably reduced when compared with controls. Results of the zebrafish embryotoxicity assay showed a decreased overall toxicity, particularly cardiotoxicity and increased safety of the nanocomposite in comparison to doxorubicin alone, as manifested by a higher survival of embryos and less pericardial edema.",
journal = "RSC Advances",
title = "Fullerenol nanoparticles as a new delivery system for doxorubicin",
volume = "6",
number = "45",
pages = "38563-38578",
doi = "10.1039/c6ra03879d"
}

Jović, D. S., Seke, M., Đorđević, A. N., Mrđanović, J. Ž., Aleksić, L. D., Bogdanović, G. M., Pavić, A. B.,& Plavec, J.. (2016). Fullerenol nanoparticles as a new delivery system for doxorubicin. in RSC Advances, 6(45), 38563-38578.
https://doi.org/10.1039/c6ra03879d

Jović DS, Seke M, Đorđević AN, Mrđanović JŽ, Aleksić LD, Bogdanović GM, Pavić AB, Plavec J. Fullerenol nanoparticles as a new delivery system for doxorubicin. in RSC Advances. 2016;6(45):38563-38578.
doi:10.1039/c6ra03879d .

Jović, Danica S., Seke, Mariana, Đorđević, Aleksandar N., Mrđanović, Jasminka Ž., Aleksić, Lidija D., Bogdanović, Gordana M., Pavić, Aleksandar B., Plavec, Janez, "Fullerenol nanoparticles as a new delivery system for doxorubicin" in RSC Advances, 6, no. 45 (2016):38563-38578,
https://doi.org/10.1039/c6ra03879d . .

TY  - JOUR
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Đorđević, Aleksandar N.
AU  - Jović, Danica S.
AU  - Labudović-Borović, Milica
AU  - Kanački, Zdenko
AU  - Janković, Milan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1306
AB  - Fullerenol (C-60(OH)(24)) is present in aqueous solutions in the form of polyanion nanoparticles with particles size distribution within the range from 15 to 42 nm. In this research it is assumed that these features could enable fullerenol nanoparticles (FNPs) to bind positively charged molecules like doxorubicin (DOX) and serve as drug carriers. Considering this, fullerenol/doxorubicin nanocomposite (FNP/DOX) is formed and characterized by ultra-performance liquid chromatography tandem mass spectrometry, dynamic light scattering, atomic force microscopy and transmission electron microscopy. Measurements have shown that DOX did not significantly affect particle size (23 nm). It is also assumed that FNP/DOX could reduce the acute cardiotoxic effects of DOX in vivo (Wistar rats treated i.p.). In this study, quantitative real time polymerase chain reaction results have shown that treatment with DOX alone caused significant increase in mRNA levels of catalase (p LT 0.05) enzyme indicating the presence of oxidative stress. This effect is significantly reduced by the treatment with FNP/DOX (p LT 0.05). Furthermore, mRNA levels of antiapoptotic enzyme (Bcl-2) are significantly increased (p LT 0.05) in all treated groups, particularly where FNP/DOX was applied, suggesting cell resistance to apoptosis. Moreover, ultrastructural analysis has shown the absence of myelin figures within the mitochondria in the heart tissue with FNP/DOX treatment, indicating reduction of oxidative stress. Hence, our results have implied that FNP/DOX is generally less harmful to the heart compared to DOX.
T2  - Nanotechnology
T1  - Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue
VL  - 27
IS  - 48
DO  - 10.1088/0957-4484/27/48/485101
ER  - 

@article{
author = "Seke, Mariana and Petrović, Danijela and Đorđević, Aleksandar N. and Jović, Danica S. and Labudović-Borović, Milica and Kanački, Zdenko and Janković, Milan",
year = "2016",
abstract = "Fullerenol (C-60(OH)(24)) is present in aqueous solutions in the form of polyanion nanoparticles with particles size distribution within the range from 15 to 42 nm. In this research it is assumed that these features could enable fullerenol nanoparticles (FNPs) to bind positively charged molecules like doxorubicin (DOX) and serve as drug carriers. Considering this, fullerenol/doxorubicin nanocomposite (FNP/DOX) is formed and characterized by ultra-performance liquid chromatography tandem mass spectrometry, dynamic light scattering, atomic force microscopy and transmission electron microscopy. Measurements have shown that DOX did not significantly affect particle size (23 nm). It is also assumed that FNP/DOX could reduce the acute cardiotoxic effects of DOX in vivo (Wistar rats treated i.p.). In this study, quantitative real time polymerase chain reaction results have shown that treatment with DOX alone caused significant increase in mRNA levels of catalase (p LT 0.05) enzyme indicating the presence of oxidative stress. This effect is significantly reduced by the treatment with FNP/DOX (p LT 0.05). Furthermore, mRNA levels of antiapoptotic enzyme (Bcl-2) are significantly increased (p LT 0.05) in all treated groups, particularly where FNP/DOX was applied, suggesting cell resistance to apoptosis. Moreover, ultrastructural analysis has shown the absence of myelin figures within the mitochondria in the heart tissue with FNP/DOX treatment, indicating reduction of oxidative stress. Hence, our results have implied that FNP/DOX is generally less harmful to the heart compared to DOX.",
journal = "Nanotechnology",
title = "Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue",
volume = "27",
number = "48",
doi = "10.1088/0957-4484/27/48/485101"
}

Seke, M., Petrović, D., Đorđević, A. N., Jović, D. S., Labudović-Borović, M., Kanački, Z.,& Janković, M.. (2016). Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue. in Nanotechnology, 27(48).
https://doi.org/10.1088/0957-4484/27/48/485101

Seke M, Petrović D, Đorđević AN, Jović DS, Labudović-Borović M, Kanački Z, Janković M. Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue. in Nanotechnology. 2016;27(48).
doi:10.1088/0957-4484/27/48/485101 .

Seke, Mariana, Petrović, Danijela, Đorđević, Aleksandar N., Jović, Danica S., Labudović-Borović, Milica, Kanački, Zdenko, Janković, Milan, "Fullerenol/doxorubicin nanocomposite mitigates acute oxidative stress and modulates apoptosis in myocardial tissue" in Nanotechnology, 27, no. 48 (2016),
https://doi.org/10.1088/0957-4484/27/48/485101 . .

TY  - CONF
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Labudović-Borović, Milica
AU  - Jović, Danica S.
AU  - Srđenović, Branislava U.
AU  - Kanacki, Zdenko
AU  - Zikić, Dragan
AU  - Đorđević, Aleksandar N.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7081
C3  - FEBS Journal
T1  - Effects of a fullerenol/doxorubicin nanocomposite on the heart tissue of healthy rats
VL  - 282
SP  - 242
EP  - 242
DO  - 10.1111/febs.13339
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7081
ER  - 

@conference{
author = "Seke, Mariana and Petrović, Danijela and Labudović-Borović, Milica and Jović, Danica S. and Srđenović, Branislava U. and Kanacki, Zdenko and Zikić, Dragan and Đorđević, Aleksandar N.",
year = "2015",
journal = "FEBS Journal",
title = "Effects of a fullerenol/doxorubicin nanocomposite on the heart tissue of healthy rats",
volume = "282",
pages = "242-242",
doi = "10.1111/febs.13339",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7081"
}

Seke, M., Petrović, D., Labudović-Borović, M., Jović, D. S., Srđenović, B. U., Kanacki, Z., Zikić, D.,& Đorđević, A. N.. (2015). Effects of a fullerenol/doxorubicin nanocomposite on the heart tissue of healthy rats. in FEBS Journal, 282, 242-242.
https://doi.org/10.1111/febs.13339
https://hdl.handle.net/21.15107/rcub_vinar_7081

Seke M, Petrović D, Labudović-Borović M, Jović DS, Srđenović BU, Kanacki Z, Zikić D, Đorđević AN. Effects of a fullerenol/doxorubicin nanocomposite on the heart tissue of healthy rats. in FEBS Journal. 2015;282:242-242.
doi:10.1111/febs.13339
https://hdl.handle.net/21.15107/rcub_vinar_7081 .

Seke, Mariana, Petrović, Danijela, Labudović-Borović, Milica, Jović, Danica S., Srđenović, Branislava U., Kanacki, Zdenko, Zikić, Dragan, Đorđević, Aleksandar N., "Effects of a fullerenol/doxorubicin nanocomposite on the heart tissue of healthy rats" in FEBS Journal, 282 (2015):242-242,
https://doi.org/10.1111/febs.13339 .,
https://hdl.handle.net/21.15107/rcub_vinar_7081 .

TY  - JOUR
AU  - Đorđević, Aleksandar N.
AU  - Ignjatović, Nenad L.
AU  - Seke, Mariana
AU  - Jović, Danica S.
AU  - Uskoković, Dragan
AU  - Rakočević, Zlatko Lj.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/213
AB  - Fullerenols are polyhydroxylated, water soluble derivatives of fullerene C-60, with potential application in medicine as diagnostic agents, antioxidants or nano drug carriers. This paper describes synthesis and physical characterization of a new nanocomposite hydroxyapatite/fullerenol. Surface of the nanocomposite hydroxyapatite/fullerenol is inhomogeneous with the diameter of the particles in the range from 100 nm to 350 nm. The zeta potential of this nanocomposite is ten times lower when compared to hydroxyapatite. Surface phosphate groups of hydroxyapatite are prone to forming hydrogen bonds, when in close contact with hydroxyl groups, which could lead to formation of hydrogen bonds between hydroxyapatite and hydroxyl groups of fullerenol. The surface of hydroxyapatite particles (-2.5 mV) was modified by fullerenol particles, as confirmed by the obtained zeta potential value of the nanocomposite biomaterial hydroxyapatite/fullerenol (-25.0 mV).
T2  - Journal of Nanoscience and Nanotechnology
T1  - Synthesis and Characterization of Hydroxyapatite/Fullerenol Nanocomposites
VL  - 15
IS  - 2
SP  - 1538
EP  - 1542
DO  - 10.1166/jnn.2015.8671
ER  - 

@article{
author = "Đorđević, Aleksandar N. and Ignjatović, Nenad L. and Seke, Mariana and Jović, Danica S. and Uskoković, Dragan and Rakočević, Zlatko Lj.",
year = "2015",
abstract = "Fullerenols are polyhydroxylated, water soluble derivatives of fullerene C-60, with potential application in medicine as diagnostic agents, antioxidants or nano drug carriers. This paper describes synthesis and physical characterization of a new nanocomposite hydroxyapatite/fullerenol. Surface of the nanocomposite hydroxyapatite/fullerenol is inhomogeneous with the diameter of the particles in the range from 100 nm to 350 nm. The zeta potential of this nanocomposite is ten times lower when compared to hydroxyapatite. Surface phosphate groups of hydroxyapatite are prone to forming hydrogen bonds, when in close contact with hydroxyl groups, which could lead to formation of hydrogen bonds between hydroxyapatite and hydroxyl groups of fullerenol. The surface of hydroxyapatite particles (-2.5 mV) was modified by fullerenol particles, as confirmed by the obtained zeta potential value of the nanocomposite biomaterial hydroxyapatite/fullerenol (-25.0 mV).",
journal = "Journal of Nanoscience and Nanotechnology",
title = "Synthesis and Characterization of Hydroxyapatite/Fullerenol Nanocomposites",
volume = "15",
number = "2",
pages = "1538-1542",
doi = "10.1166/jnn.2015.8671"
}

Đorđević, A. N., Ignjatović, N. L., Seke, M., Jović, D. S., Uskoković, D.,& Rakočević, Z. Lj.. (2015). Synthesis and Characterization of Hydroxyapatite/Fullerenol Nanocomposites. in Journal of Nanoscience and Nanotechnology, 15(2), 1538-1542.
https://doi.org/10.1166/jnn.2015.8671

Đorđević AN, Ignjatović NL, Seke M, Jović DS, Uskoković D, Rakočević ZL. Synthesis and Characterization of Hydroxyapatite/Fullerenol Nanocomposites. in Journal of Nanoscience and Nanotechnology. 2015;15(2):1538-1542.
doi:10.1166/jnn.2015.8671 .

Đorđević, Aleksandar N., Ignjatović, Nenad L., Seke, Mariana, Jović, Danica S., Uskoković, Dragan, Rakočević, Zlatko Lj., "Synthesis and Characterization of Hydroxyapatite/Fullerenol Nanocomposites" in Journal of Nanoscience and Nanotechnology, 15, no. 2 (2015):1538-1542,
https://doi.org/10.1166/jnn.2015.8671 . .

TY  - JOUR
AU  - Petrović, Danijela
AU  - Seke, Mariana
AU  - Srđenović, Branislava U.
AU  - Đorđević, Aleksandar N.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/698
AB  - Fullerenes are molecules that, due to their unique structure, have very specific chemical properties which offer them very wide array of applications in nanomedicine. The most prominent are protection from radiation-induced injury, neuroprotection, drug and gene delivery, anticancer therapy, adjuvant within different treatments, photosensitizing, sonosensitizing, bone reparation, and biosensing. However, it is of crucial importance to be elucidated how fullerenes immunomodulate human system of defense. In addition, the most current research, merging immunology and nanomedicine, results in development of nanovaccines, which may represent the milestone of future treatment of diseases.
T2  - Journal of Nanomaterials
T1  - Applications of Anti/Prooxidant Fullerenes in Nanomedicine along with Fullerenes Influence on the Immune System
DO  - 10.1155/2015/565638
ER  - 

@article{
author = "Petrović, Danijela and Seke, Mariana and Srđenović, Branislava U. and Đorđević, Aleksandar N.",
year = "2015",
abstract = "Fullerenes are molecules that, due to their unique structure, have very specific chemical properties which offer them very wide array of applications in nanomedicine. The most prominent are protection from radiation-induced injury, neuroprotection, drug and gene delivery, anticancer therapy, adjuvant within different treatments, photosensitizing, sonosensitizing, bone reparation, and biosensing. However, it is of crucial importance to be elucidated how fullerenes immunomodulate human system of defense. In addition, the most current research, merging immunology and nanomedicine, results in development of nanovaccines, which may represent the milestone of future treatment of diseases.",
journal = "Journal of Nanomaterials",
title = "Applications of Anti/Prooxidant Fullerenes in Nanomedicine along with Fullerenes Influence on the Immune System",
doi = "10.1155/2015/565638"
}

Petrović, D., Seke, M., Srđenović, B. U.,& Đorđević, A. N.. (2015). Applications of Anti/Prooxidant Fullerenes in Nanomedicine along with Fullerenes Influence on the Immune System. in Journal of Nanomaterials.
https://doi.org/10.1155/2015/565638

Petrović D, Seke M, Srđenović BU, Đorđević AN. Applications of Anti/Prooxidant Fullerenes in Nanomedicine along with Fullerenes Influence on the Immune System. in Journal of Nanomaterials. 2015;.
doi:10.1155/2015/565638 .

Petrović, Danijela, Seke, Mariana, Srđenović, Branislava U., Đorđević, Aleksandar N., "Applications of Anti/Prooxidant Fullerenes in Nanomedicine along with Fullerenes Influence on the Immune System" in Journal of Nanomaterials (2015),
https://doi.org/10.1155/2015/565638 . .

TY  - JOUR
AU  - Đorđević, Aleksandar N.
AU  - Srđenović, Branislava U.
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Injac, Rade
AU  - Mrđanović, Jasminka Ž.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/699
AB  - This review describes the chemical synthesis of polar polyhydroxylated fullerene C-60 derivatives, fullerenols C-60(OH)(n), 2 LT = n LT = 44, C-60 HzOx(OH)(y), and polyanion fullerenols C-60(OH)(15)(ONa)(9), ranging from the very first synthetic methods up to some contemporary approaches to synthesis and separation. It also provides some basic information about physical characteristics of fullerenols. With the increasing number of hydroxyl groups, water solubility of fullerenols increases as well. Fullerenols both in water and biological media build nanoparticles of different dimensions and stability. In different chemical and biological model systems a large number of various polyhydroxylated fullerene derivatives were tested and they showed both their antioxidative and prooxidative characteristics. Several mechanisms have been proposed for the antioxidant activity of fullerenol. In addition, this paper also provides insight into patents referring to the antioxidant properties of fullerenol.
T2  - Journal of Nanomaterials
T1  - Review of Synthesis and Antioxidant Potential of Fullerenol Nanoparticles
DO  - 10.1155/2015/567073
ER  - 

@article{
author = "Đorđević, Aleksandar N. and Srđenović, Branislava U. and Seke, Mariana and Petrović, Danijela and Injac, Rade and Mrđanović, Jasminka Ž.",
year = "2015",
abstract = "This review describes the chemical synthesis of polar polyhydroxylated fullerene C-60 derivatives, fullerenols C-60(OH)(n), 2 LT = n LT = 44, C-60 HzOx(OH)(y), and polyanion fullerenols C-60(OH)(15)(ONa)(9), ranging from the very first synthetic methods up to some contemporary approaches to synthesis and separation. It also provides some basic information about physical characteristics of fullerenols. With the increasing number of hydroxyl groups, water solubility of fullerenols increases as well. Fullerenols both in water and biological media build nanoparticles of different dimensions and stability. In different chemical and biological model systems a large number of various polyhydroxylated fullerene derivatives were tested and they showed both their antioxidative and prooxidative characteristics. Several mechanisms have been proposed for the antioxidant activity of fullerenol. In addition, this paper also provides insight into patents referring to the antioxidant properties of fullerenol.",
journal = "Journal of Nanomaterials",
title = "Review of Synthesis and Antioxidant Potential of Fullerenol Nanoparticles",
doi = "10.1155/2015/567073"
}

Đorđević, A. N., Srđenović, B. U., Seke, M., Petrović, D., Injac, R.,& Mrđanović, J. Ž.. (2015). Review of Synthesis and Antioxidant Potential of Fullerenol Nanoparticles. in Journal of Nanomaterials.
https://doi.org/10.1155/2015/567073

Đorđević AN, Srđenović BU, Seke M, Petrović D, Injac R, Mrđanović JŽ. Review of Synthesis and Antioxidant Potential of Fullerenol Nanoparticles. in Journal of Nanomaterials. 2015;.
doi:10.1155/2015/567073 .

Đorđević, Aleksandar N., Srđenović, Branislava U., Seke, Mariana, Petrović, Danijela, Injac, Rade, Mrđanović, Jasminka Ž., "Review of Synthesis and Antioxidant Potential of Fullerenol Nanoparticles" in Journal of Nanomaterials (2015),
https://doi.org/10.1155/2015/567073 . .

TY  - JOUR
AU  - Srđenović, Branislava U.
AU  - Slavic, Marija N.
AU  - Stankov, Karmen M.
AU  - Kladar, Nebojsa V.
AU  - Jović, Danica S.
AU  - Seke, Mariana
AU  - Bogdanović, Višnja V.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/767
AB  - Fullerenol (C-60(OH)(24)) nanoparticles (FNP) have a significant role in biomedical research due to their numerous biological activities, some of which have cytoprotective and anti-oxidative properties. The aim of this study was to measure distribution of fullerenol nanoparticles and zeta potential in cell medium RPMI 1640 with 10% fetal bovine serum (FBS) and to investigate the influence of FNP on Chinese hamster ovary cells (CHO-K1) survival, as well as to determine the activity of three antioxidative enzymes: superoxide-dismutase, glutathione-reductase and glutathione-S-transferase in mitomycin C-treated cell line. Our investigation implies that FNP, as a strong antioxidant, influences the cellular redox state and enzyme activities and thus may reduce cell proliferation, which confirms that FNP could be exploited for its use as a cytoprotective agent.
T2  - Hemijska industrija
T1  - Size distribution of fullerenol nanoparticles in cell culture medium and their influence on antioxidative enzymes in Chinese hamster ovary cells
VL  - 69
IS  - 4
SP  - 425
EP  - 431
DO  - 10.2298/HEMIND131218054S
ER  - 

@article{
author = "Srđenović, Branislava U. and Slavic, Marija N. and Stankov, Karmen M. and Kladar, Nebojsa V. and Jović, Danica S. and Seke, Mariana and Bogdanović, Višnja V.",
year = "2015",
abstract = "Fullerenol (C-60(OH)(24)) nanoparticles (FNP) have a significant role in biomedical research due to their numerous biological activities, some of which have cytoprotective and anti-oxidative properties. The aim of this study was to measure distribution of fullerenol nanoparticles and zeta potential in cell medium RPMI 1640 with 10% fetal bovine serum (FBS) and to investigate the influence of FNP on Chinese hamster ovary cells (CHO-K1) survival, as well as to determine the activity of three antioxidative enzymes: superoxide-dismutase, glutathione-reductase and glutathione-S-transferase in mitomycin C-treated cell line. Our investigation implies that FNP, as a strong antioxidant, influences the cellular redox state and enzyme activities and thus may reduce cell proliferation, which confirms that FNP could be exploited for its use as a cytoprotective agent.",
journal = "Hemijska industrija",
title = "Size distribution of fullerenol nanoparticles in cell culture medium and their influence on antioxidative enzymes in Chinese hamster ovary cells",
volume = "69",
number = "4",
pages = "425-431",
doi = "10.2298/HEMIND131218054S"
}

Srđenović, B. U., Slavic, M. N., Stankov, K. M., Kladar, N. V., Jović, D. S., Seke, M.,& Bogdanović, V. V.. (2015). Size distribution of fullerenol nanoparticles in cell culture medium and their influence on antioxidative enzymes in Chinese hamster ovary cells. in Hemijska industrija, 69(4), 425-431.
https://doi.org/10.2298/HEMIND131218054S

Srđenović BU, Slavic MN, Stankov KM, Kladar NV, Jović DS, Seke M, Bogdanović VV. Size distribution of fullerenol nanoparticles in cell culture medium and their influence on antioxidative enzymes in Chinese hamster ovary cells. in Hemijska industrija. 2015;69(4):425-431.
doi:10.2298/HEMIND131218054S .

Srđenović, Branislava U., Slavic, Marija N., Stankov, Karmen M., Kladar, Nebojsa V., Jović, Danica S., Seke, Mariana, Bogdanović, Višnja V., "Size distribution of fullerenol nanoparticles in cell culture medium and their influence on antioxidative enzymes in Chinese hamster ovary cells" in Hemijska industrija, 69, no. 4 (2015):425-431,
https://doi.org/10.2298/HEMIND131218054S . .

TY  - CHAP
AU  - Đorđević, Aleksandar N.
AU  - Injac, Rade
AU  - Jović, Danica
AU  - Mrđanović, Jasminka Ž.
AU  - Seke, Mariana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12696
AB  - The unique size-dependent properties of carbon nanomaterials (CNMs)- graphene, nanotubes (CNTs) and fullerenes- make them very attractive for diagnostic and therapeutic application. This chapter presents possible application of CNMs. Graphene with extraordinary chemical and physical properties has already revealed a great number of potential applications such as environmental toxic material removal, drug delivery, tissue engineering, and fl uorescence-based biomolecular sensing. The CNT derivatives have many interesting properties which make them potentially useful in a living system as biosensors, bioelectronic devices based on enzyme-nanotube or antibody-nanotube conjugates, chemotherapeutic agents, hyperthermia therapy and immunotherapy agents, agent in treatment of central nervous system disorders, and tissue engineering agent. Fullerene C60 derivatives have been used as: drug and gene delivery vectors, magnetic resonance imaging agents, radio protectors, antioxidants, HIV-1 protease inhibitors, antigenotoxic agents, and phototherapy agents. Fullerenols are polyhydroxylated derivatives of fullerene (C60(OH)n) with remarkable antioxidant, xenobiotic-protective, radioprotective, nanodrug and endohedral gadolinium carrier properties.
T2  - Advanced Carbon Materials and Technology
T1  - Bioimpact of Carbon Nanomaterials
SP  - 193
EP  - 271
DO  - 10.1002/9781118895399.ch6
ER  - 

@inbook{
author = "Đorđević, Aleksandar N. and Injac, Rade and Jović, Danica and Mrđanović, Jasminka Ž. and Seke, Mariana",
year = "2014",
abstract = "The unique size-dependent properties of carbon nanomaterials (CNMs)- graphene, nanotubes (CNTs) and fullerenes- make them very attractive for diagnostic and therapeutic application. This chapter presents possible application of CNMs. Graphene with extraordinary chemical and physical properties has already revealed a great number of potential applications such as environmental toxic material removal, drug delivery, tissue engineering, and fl uorescence-based biomolecular sensing. The CNT derivatives have many interesting properties which make them potentially useful in a living system as biosensors, bioelectronic devices based on enzyme-nanotube or antibody-nanotube conjugates, chemotherapeutic agents, hyperthermia therapy and immunotherapy agents, agent in treatment of central nervous system disorders, and tissue engineering agent. Fullerene C60 derivatives have been used as: drug and gene delivery vectors, magnetic resonance imaging agents, radio protectors, antioxidants, HIV-1 protease inhibitors, antigenotoxic agents, and phototherapy agents. Fullerenols are polyhydroxylated derivatives of fullerene (C60(OH)n) with remarkable antioxidant, xenobiotic-protective, radioprotective, nanodrug and endohedral gadolinium carrier properties.",
journal = "Advanced Carbon Materials and Technology",
booktitle = "Bioimpact of Carbon Nanomaterials",
pages = "193-271",
doi = "10.1002/9781118895399.ch6"
}

Đorđević, A. N., Injac, R., Jović, D., Mrđanović, J. Ž.,& Seke, M.. (2014). Bioimpact of Carbon Nanomaterials. in Advanced Carbon Materials and Technology, 193-271.
https://doi.org/10.1002/9781118895399.ch6

Đorđević AN, Injac R, Jović D, Mrđanović JŽ, Seke M. Bioimpact of Carbon Nanomaterials. in Advanced Carbon Materials and Technology. 2014;:193-271.
doi:10.1002/9781118895399.ch6 .

Đorđević, Aleksandar N., Injac, Rade, Jović, Danica, Mrđanović, Jasminka Ž., Seke, Mariana, "Bioimpact of Carbon Nanomaterials" in Advanced Carbon Materials and Technology (2014):193-271,
https://doi.org/10.1002/9781118895399.ch6 . .

TY  - JOUR
AU  - Labudović-Borović, Milica
AU  - Icevic, Ivana
AU  - Kanački, Zdenko
AU  - Zikic, Dragan
AU  - Seke, Mariana
AU  - Injac, Rade
AU  - Đorđević, Aleksandar N.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5924
AB  - Cardioprotective effects of fullerenol C-60(OH)(24) nanoparticles (FNP) were investigated in pigs after a single treatment with doxorubicin (DOX). Semithin and ultrathin sections of myocardial tissue routinely prepared for transmission electron microscopy were analyzed. Extensive intracellular damage was confirmed in cardiomyocytes of DOX-treated animals. By means of ultrastructural analysis, a certain degree of parenchymal degeneration was confirmed even in animals treated with FNP alone, including both the oral and the intraperitoneal application of the substance. The cardioprotective effects of FNP in animals previously treated with DOX were recognized to a certain extent, but were not fully confirmed at the ultrastructural level. Nevertheless, the myocardial morphology of DOX-treated animals improved after the admission of FNP. Irregular orientation of myofibrils, myofibrillar disruption, intracellular edema, and vacuolization were reduced, but not completely eliminated. Reduction of these cellular alterations was achieved if FNP was applied orally 6 h prior to DOX treatment in a dose of 18 mg/kg. However, numerous defects, including the inner mitochondrial membrane and the plasma membrane disruption of certain cells persisted. In FNP/DOX-treated animals, the presence of multinuclear cells with mitosis-like figures resembling metaphase or anaphase were observed, indicating that DOX and FNP could have a complex influence on the cell cycle of cardiomyocytes. Based on this experiment, further careful increase in dosage may be advised to enhance FNP-induced cardioprotection. These investigations should, however, always be combined with ultrastructural analysis. The FNP/DOX interaction is an excellent model for the investigation of cardiomyocyte cell death and cell cycle mechanisms.
T2  - Ultrastructural Pathology
T1  - Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study
VL  - 38
IS  - 2
SP  - 150
EP  - 163
DO  - 10.3109/01913123.2013.822045
ER  - 

@article{
author = "Labudović-Borović, Milica and Icevic, Ivana and Kanački, Zdenko and Zikic, Dragan and Seke, Mariana and Injac, Rade and Đorđević, Aleksandar N.",
year = "2014",
abstract = "Cardioprotective effects of fullerenol C-60(OH)(24) nanoparticles (FNP) were investigated in pigs after a single treatment with doxorubicin (DOX). Semithin and ultrathin sections of myocardial tissue routinely prepared for transmission electron microscopy were analyzed. Extensive intracellular damage was confirmed in cardiomyocytes of DOX-treated animals. By means of ultrastructural analysis, a certain degree of parenchymal degeneration was confirmed even in animals treated with FNP alone, including both the oral and the intraperitoneal application of the substance. The cardioprotective effects of FNP in animals previously treated with DOX were recognized to a certain extent, but were not fully confirmed at the ultrastructural level. Nevertheless, the myocardial morphology of DOX-treated animals improved after the admission of FNP. Irregular orientation of myofibrils, myofibrillar disruption, intracellular edema, and vacuolization were reduced, but not completely eliminated. Reduction of these cellular alterations was achieved if FNP was applied orally 6 h prior to DOX treatment in a dose of 18 mg/kg. However, numerous defects, including the inner mitochondrial membrane and the plasma membrane disruption of certain cells persisted. In FNP/DOX-treated animals, the presence of multinuclear cells with mitosis-like figures resembling metaphase or anaphase were observed, indicating that DOX and FNP could have a complex influence on the cell cycle of cardiomyocytes. Based on this experiment, further careful increase in dosage may be advised to enhance FNP-induced cardioprotection. These investigations should, however, always be combined with ultrastructural analysis. The FNP/DOX interaction is an excellent model for the investigation of cardiomyocyte cell death and cell cycle mechanisms.",
journal = "Ultrastructural Pathology",
title = "Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study",
volume = "38",
number = "2",
pages = "150-163",
doi = "10.3109/01913123.2013.822045"
}

Labudović-Borović, M., Icevic, I., Kanački, Z., Zikic, D., Seke, M., Injac, R.,& Đorđević, A. N.. (2014). Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study. in Ultrastructural Pathology, 38(2), 150-163.
https://doi.org/10.3109/01913123.2013.822045

Labudović-Borović M, Icevic I, Kanački Z, Zikic D, Seke M, Injac R, Đorđević AN. Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study. in Ultrastructural Pathology. 2014;38(2):150-163.
doi:10.3109/01913123.2013.822045 .

Labudović-Borović, Milica, Icevic, Ivana, Kanački, Zdenko, Zikic, Dragan, Seke, Mariana, Injac, Rade, Đorđević, Aleksandar N., "Effects of Fullerenol C-60(OH)(24) Nanoparticles on a Single-dose Doxorubicin-induced Cardiotoxicity in Pigs: An Ultrastructural Study" in Ultrastructural Pathology, 38, no. 2 (2014):150-163,
https://doi.org/10.3109/01913123.2013.822045 . .

TY  - CONF
AU  - Đorđević, Aleksandar
AU  - Seke, Mariana
AU  - Trpkov, Đorđe
AU  - Cvetićanin, Jelena
AU  - Rogić Miladinović, Zorana
AU  - Aćimović, Danka
AU  - Demajo, Miroslav
AU  - Nešković, Olivera
AU  - Rakočević, Zlatko
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12409
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - 3rd Workshop Specific Methods for Food Safety and Quality : Proceedings
T1  - MALDI TOF and AFM study of Doxorubicin-Fullerenol Nanocomposite
SP  - 34
EP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12409
ER  - 

@conference{
author = "Đorđević, Aleksandar and Seke, Mariana and Trpkov, Đorđe and Cvetićanin, Jelena and Rogić Miladinović, Zorana and Aćimović, Danka and Demajo, Miroslav and Nešković, Olivera and Rakočević, Zlatko",
year = "2012",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "3rd Workshop Specific Methods for Food Safety and Quality : Proceedings",
title = "MALDI TOF and AFM study of Doxorubicin-Fullerenol Nanocomposite",
pages = "34-38",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12409"
}

Đorđević, A., Seke, M., Trpkov, Đ., Cvetićanin, J., Rogić Miladinović, Z., Aćimović, D., Demajo, M., Nešković, O.,& Rakočević, Z.. (2012). MALDI TOF and AFM study of Doxorubicin-Fullerenol Nanocomposite. in 3rd Workshop Specific Methods for Food Safety and Quality : Proceedings
Belgrade : Vinča Institute of Nuclear Sciences., 34-38.
https://hdl.handle.net/21.15107/rcub_vinar_12409

Đorđević A, Seke M, Trpkov Đ, Cvetićanin J, Rogić Miladinović Z, Aćimović D, Demajo M, Nešković O, Rakočević Z. MALDI TOF and AFM study of Doxorubicin-Fullerenol Nanocomposite. in 3rd Workshop Specific Methods for Food Safety and Quality : Proceedings. 2012;:34-38.
https://hdl.handle.net/21.15107/rcub_vinar_12409 .

Đorđević, Aleksandar, Seke, Mariana, Trpkov, Đorđe, Cvetićanin, Jelena, Rogić Miladinović, Zorana, Aćimović, Danka, Demajo, Miroslav, Nešković, Olivera, Rakočević, Zlatko, "MALDI TOF and AFM study of Doxorubicin-Fullerenol Nanocomposite" in 3rd Workshop Specific Methods for Food Safety and Quality : Proceedings (2012):34-38,
https://hdl.handle.net/21.15107/rcub_vinar_12409 .

TY  - CONF
AU  - Seke, Mariana
AU  - Popadić, Dušan
AU  - Isaković, Aleksandra
AU  - Marković, Ivanka
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12773
AB  - Mnoge ćelije u CNS-u preuzimaju nukleozide iz vanćelijske teĉnosti i koriste ih za sintezu nukleotida u putevima uštede. Nukleozidi se kroz membranu astrocita transportuje putem dve genske familije nukleozidnih transportera: ekvilibrativnih (ENT1 i ENT2) koji omogućavaju jednosmernu olakšanu difuziju i koncentrativnih (CNT2) koji omogućavaju jednosmerni sekundarno aktivni transport kroz ćelijsku membranu. Cilj ovog istraţivanja je bio ispitivanje ekspresije razliĉitih klasa nukleozidnih transportera na primarno kultivisanim astrocitima pacova, kao i uticaj hroniĉno povećane koncentracije adenozina u vanćelijskom medijumu na stepen njihove ekspresije. Eksperimenti su raĊeni u primarnoj kulturi astrocita pacova soja Wistar. Ćelije su u toku 21 dana kontinuirano tretirane adenozinom u koncentracijama od 25µM i 100µM. Kvantifikacija genske ekspresije je vršena RT-qPCR analizom na svakih 7 dana i izraţena je kao ΔCt vrednost u odmosu na vrednost koamplifikovanog β-aktina. Rezultati su pokazali da se u primarnim astrocitima pacova eksprimiraju transporteri i ekvilibrativnog i koncentrativnog tipa. Najveću ekspresiju nakon 7-og dana u kulturi je pokazao koncentrativni transporter CNT 2 (ΔCt=7,13±0,42), dok je stepen ekspresije za ekvilibrativne transportere bio nešto niţi za ENT 1 ΔCt=9,48±0,29, a za ENT 2 ΔCt=8,67±0,24. TakoĊe je uoĉeno da se tokom kultivisanja u vremenskom periodu od 21-og dana postepeno smanjuje genska ekspresija svih ispitivanih transportera: za CNT 2 ΔCt=12,75±0,51; za ENT 1 ΔCt=12,78±0,41; i za ENT 2 ΔCt=12,37±0,44. U uslovima kontinuiranog prisustva adenozina u primarnoj kulturi astrocita (25µM, odnosno 100µM) nakon 7,14 i 21 dana, nije došlo do znaĉajne promene u ekpresiji ni jednog od ispitivanih transportera u odnosnu na populaciju ćelija koja nije bila izloţena tretmanu adenozinom.
C3  - VII/XIII Kongres neurologa Srbije sa međunarodnim uĉešćem : IV Kongres društva za neuronauke Srbije sa međunarodnim uĉešćem : I Simpozijum neuroloških medicinskih sestara - Tehniĉara Srbije : Zbornik sažetaka
T1  - Ekspresija nukleozidnih transportera u primarnoj kulturi astrocita pacova
SP  - 381
EP  - 382
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12773
ER  - 

@conference{
author = "Seke, Mariana and Popadić, Dušan and Isaković, Aleksandra and Marković, Ivanka",
year = "2008",
abstract = "Mnoge ćelije u CNS-u preuzimaju nukleozide iz vanćelijske teĉnosti i koriste ih za sintezu nukleotida u putevima uštede. Nukleozidi se kroz membranu astrocita transportuje putem dve genske familije nukleozidnih transportera: ekvilibrativnih (ENT1 i ENT2) koji omogućavaju jednosmernu olakšanu difuziju i koncentrativnih (CNT2) koji omogućavaju jednosmerni sekundarno aktivni transport kroz ćelijsku membranu. Cilj ovog istraţivanja je bio ispitivanje ekspresije razliĉitih klasa nukleozidnih transportera na primarno kultivisanim astrocitima pacova, kao i uticaj hroniĉno povećane koncentracije adenozina u vanćelijskom medijumu na stepen njihove ekspresije. Eksperimenti su raĊeni u primarnoj kulturi astrocita pacova soja Wistar. Ćelije su u toku 21 dana kontinuirano tretirane adenozinom u koncentracijama od 25µM i 100µM. Kvantifikacija genske ekspresije je vršena RT-qPCR analizom na svakih 7 dana i izraţena je kao ΔCt vrednost u odmosu na vrednost koamplifikovanog β-aktina. Rezultati su pokazali da se u primarnim astrocitima pacova eksprimiraju transporteri i ekvilibrativnog i koncentrativnog tipa. Najveću ekspresiju nakon 7-og dana u kulturi je pokazao koncentrativni transporter CNT 2 (ΔCt=7,13±0,42), dok je stepen ekspresije za ekvilibrativne transportere bio nešto niţi za ENT 1 ΔCt=9,48±0,29, a za ENT 2 ΔCt=8,67±0,24. TakoĊe je uoĉeno da se tokom kultivisanja u vremenskom periodu od 21-og dana postepeno smanjuje genska ekspresija svih ispitivanih transportera: za CNT 2 ΔCt=12,75±0,51; za ENT 1 ΔCt=12,78±0,41; i za ENT 2 ΔCt=12,37±0,44. U uslovima kontinuiranog prisustva adenozina u primarnoj kulturi astrocita (25µM, odnosno 100µM) nakon 7,14 i 21 dana, nije došlo do znaĉajne promene u ekpresiji ni jednog od ispitivanih transportera u odnosnu na populaciju ćelija koja nije bila izloţena tretmanu adenozinom.",
journal = "VII/XIII Kongres neurologa Srbije sa međunarodnim uĉešćem : IV Kongres društva za neuronauke Srbije sa međunarodnim uĉešćem : I Simpozijum neuroloških medicinskih sestara - Tehniĉara Srbije : Zbornik sažetaka",
title = "Ekspresija nukleozidnih transportera u primarnoj kulturi astrocita pacova",
pages = "381-382",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12773"
}

Seke, M., Popadić, D., Isaković, A.,& Marković, I.. (2008). Ekspresija nukleozidnih transportera u primarnoj kulturi astrocita pacova. in VII/XIII Kongres neurologa Srbije sa međunarodnim uĉešćem : IV Kongres društva za neuronauke Srbije sa međunarodnim uĉešćem : I Simpozijum neuroloških medicinskih sestara - Tehniĉara Srbije : Zbornik sažetaka, 381-382.
https://hdl.handle.net/21.15107/rcub_vinar_12773

Seke M, Popadić D, Isaković A, Marković I. Ekspresija nukleozidnih transportera u primarnoj kulturi astrocita pacova. in VII/XIII Kongres neurologa Srbije sa međunarodnim uĉešćem : IV Kongres društva za neuronauke Srbije sa međunarodnim uĉešćem : I Simpozijum neuroloških medicinskih sestara - Tehniĉara Srbije : Zbornik sažetaka. 2008;:381-382.
https://hdl.handle.net/21.15107/rcub_vinar_12773 .

Seke, Mariana, Popadić, Dušan, Isaković, Aleksandra, Marković, Ivanka, "Ekspresija nukleozidnih transportera u primarnoj kulturi astrocita pacova" in VII/XIII Kongres neurologa Srbije sa međunarodnim uĉešćem : IV Kongres društva za neuronauke Srbije sa međunarodnim uĉešćem : I Simpozijum neuroloških medicinskih sestara - Tehniĉara Srbije : Zbornik sažetaka (2008):381-382,
https://hdl.handle.net/21.15107/rcub_vinar_12773 .