TY  - JOUR
AU  - Kostic, Smiljana
AU  - Kolić, Ivana
AU  - Raičević, Ranko
AU  - Stojanovic, Zvezdana
AU  - Kostić, Dejan
AU  - Dinčić, Evica
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1626
AB  - Background/Aim. Due to the fact that there is a relatively small number of data related to systemic insulin abnormalities in the multiple sclerosis (MS), the main objective of our study was to determine whether a dysbalance of glucose and insulin metabolism exist in patients with natural course of MS. Our hypothesis was that the metabolic disorder that characterizes state of the insulin resistance (IR) and reduced insulin sensitivity (IS) in untreated patients with MS could play a role in disease progression and degree of functional disability. Methods. The study included 31 patients with relapsing-remitting (RR) MS and 14 healthy controls from the same geographic area matched by age, ethnicity and number of smokers. The glucose tolerance, IS, and IR were examined using an oral glucose tolerance test (OGTT) and using basal plasma glucose and insulin levels. The functional disability and disease progression were evaluated by the Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Severity Score (MSSS). Results. The MS patients tolerated glucose equally well as the healthy controls. Basal concentrations of insulin were significantly higher in the MS group p LT 0.05), as well as insulin plasma level 30 min after oral glucose load (p LT 0.01). The patients with MS had significantly higher values of homeostasis model assessment indexes of IR (HOMA-IR) (p = 0.027; p = 0.028). The percentage of IS (HOMA2 % S) and whole body IS index (ISI Matsuda) showed significantly lower values in the MS patients than in the controls (p = 0.005; p = 0.001). The insulinogenic index in the first 30 min of OGTT was significantly higher in MS patients (p = 0.005). The measures of functional disability and MS progression did not correlate significantly with the investigated parameters of IR and IS indexes. Conclusion. This study demonstrates for the first time the existence of hyperinsulinemia, reduced insulin sensitivity and normal glucose tolerance that indicate the initial phase of IR in the natural course of MS. Additional research is necessary in order to define the mechanisms of occurrence and the impact of IR on the complex pathophysiological processes in MS.
T2  - Vojnosanitetski pregled
T1  - Insulin resistance in drug naive patients with multiple sclerosis
VL  - 74
IS  - 6
SP  - 563
EP  - 570
DO  - 10.2298/VSP160218082K
ER  - 

@article{
author = "Kostic, Smiljana and Kolić, Ivana and Raičević, Ranko and Stojanovic, Zvezdana and Kostić, Dejan and Dinčić, Evica",
year = "2017",
abstract = "Background/Aim. Due to the fact that there is a relatively small number of data related to systemic insulin abnormalities in the multiple sclerosis (MS), the main objective of our study was to determine whether a dysbalance of glucose and insulin metabolism exist in patients with natural course of MS. Our hypothesis was that the metabolic disorder that characterizes state of the insulin resistance (IR) and reduced insulin sensitivity (IS) in untreated patients with MS could play a role in disease progression and degree of functional disability. Methods. The study included 31 patients with relapsing-remitting (RR) MS and 14 healthy controls from the same geographic area matched by age, ethnicity and number of smokers. The glucose tolerance, IS, and IR were examined using an oral glucose tolerance test (OGTT) and using basal plasma glucose and insulin levels. The functional disability and disease progression were evaluated by the Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Severity Score (MSSS). Results. The MS patients tolerated glucose equally well as the healthy controls. Basal concentrations of insulin were significantly higher in the MS group p LT 0.05), as well as insulin plasma level 30 min after oral glucose load (p LT 0.01). The patients with MS had significantly higher values of homeostasis model assessment indexes of IR (HOMA-IR) (p = 0.027; p = 0.028). The percentage of IS (HOMA2 % S) and whole body IS index (ISI Matsuda) showed significantly lower values in the MS patients than in the controls (p = 0.005; p = 0.001). The insulinogenic index in the first 30 min of OGTT was significantly higher in MS patients (p = 0.005). The measures of functional disability and MS progression did not correlate significantly with the investigated parameters of IR and IS indexes. Conclusion. This study demonstrates for the first time the existence of hyperinsulinemia, reduced insulin sensitivity and normal glucose tolerance that indicate the initial phase of IR in the natural course of MS. Additional research is necessary in order to define the mechanisms of occurrence and the impact of IR on the complex pathophysiological processes in MS.",
journal = "Vojnosanitetski pregled",
title = "Insulin resistance in drug naive patients with multiple sclerosis",
volume = "74",
number = "6",
pages = "563-570",
doi = "10.2298/VSP160218082K"
}

Kostic, S., Kolić, I., Raičević, R., Stojanovic, Z., Kostić, D.,& Dinčić, E.. (2017). Insulin resistance in drug naive patients with multiple sclerosis. in Vojnosanitetski pregled, 74(6), 563-570.
https://doi.org/10.2298/VSP160218082K

Kostic S, Kolić I, Raičević R, Stojanovic Z, Kostić D, Dinčić E. Insulin resistance in drug naive patients with multiple sclerosis. in Vojnosanitetski pregled. 2017;74(6):563-570.
doi:10.2298/VSP160218082K .

Kostic, Smiljana, Kolić, Ivana, Raičević, Ranko, Stojanovic, Zvezdana, Kostić, Dejan, Dinčić, Evica, "Insulin resistance in drug naive patients with multiple sclerosis" in Vojnosanitetski pregled, 74, no. 6 (2017):563-570,
https://doi.org/10.2298/VSP160218082K . .

TY  - JOUR
AU  - Živković, Maja
AU  - Kolaković, Ana
AU  - Stojković, Ljiljana S.
AU  - Dinčić, Evica
AU  - Kostic, Smiljana
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1016
AB  - The components of renin-angiotensin system, such as angiotensin-converting enzyme (ACE), angiotensin II and angiotensin II receptor type 1 and 2 (AT1R and AT2R), are expressed in the central nervous system and leukocytes and proposed to be involved in the inflammation and pathogenesis of multiple sclerosis (MS). ACE I/D, AT1R 1166A/C and AT2R-1332A/G are functional polymorphisms associated with phenotypes of diverse chronic inflammatory diseases. The aim of this study was to investigate the association between ACE I/D, AT1R 1166A/C and AT2R-1332A/G gene polymorphisms and MS in Serbian population. A total of 470 MS patients and 478 controls participated in the study. Allele-specific polymerase chain reaction (PCR) was performed for genotyping of the ACE polymorphism. The AT1R and AT2R genotyping was done by duplex PCR and restriction fragment length polymorphism analysis. Both ACE homozygotes, II and DD, were significantly overrepresented in MS patients, compared to controls (chi(2) test p = 0.03). Neither genotype nor allele frequencies of AT1R 1166A/C polymorphism were significantly different between patients and controls. Significant overrepresentation of AT2R-1332 AA genotype in female patients, compared to female controls, was detected (OR = 1.67, 95%CI = 1.13-2.49, chi(2) test p = 0.01), suggesting that this genotype could be a gender-specific genetic risk factor for MS. (C) 2016 Elsevier B.V. All rights reserved.
T2  - Journal of the Neurological Sciences
T1  - Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis
VL  - 363
SP  - 29
EP  - 32
DO  - 10.1016/j.jns.2016.02.026
ER  - 

@article{
author = "Živković, Maja and Kolaković, Ana and Stojković, Ljiljana S. and Dinčić, Evica and Kostic, Smiljana and Alavantić, Dragan and Stanković, Aleksandra",
year = "2016",
abstract = "The components of renin-angiotensin system, such as angiotensin-converting enzyme (ACE), angiotensin II and angiotensin II receptor type 1 and 2 (AT1R and AT2R), are expressed in the central nervous system and leukocytes and proposed to be involved in the inflammation and pathogenesis of multiple sclerosis (MS). ACE I/D, AT1R 1166A/C and AT2R-1332A/G are functional polymorphisms associated with phenotypes of diverse chronic inflammatory diseases. The aim of this study was to investigate the association between ACE I/D, AT1R 1166A/C and AT2R-1332A/G gene polymorphisms and MS in Serbian population. A total of 470 MS patients and 478 controls participated in the study. Allele-specific polymerase chain reaction (PCR) was performed for genotyping of the ACE polymorphism. The AT1R and AT2R genotyping was done by duplex PCR and restriction fragment length polymorphism analysis. Both ACE homozygotes, II and DD, were significantly overrepresented in MS patients, compared to controls (chi(2) test p = 0.03). Neither genotype nor allele frequencies of AT1R 1166A/C polymorphism were significantly different between patients and controls. Significant overrepresentation of AT2R-1332 AA genotype in female patients, compared to female controls, was detected (OR = 1.67, 95%CI = 1.13-2.49, chi(2) test p = 0.01), suggesting that this genotype could be a gender-specific genetic risk factor for MS. (C) 2016 Elsevier B.V. All rights reserved.",
journal = "Journal of the Neurological Sciences",
title = "Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis",
volume = "363",
pages = "29-32",
doi = "10.1016/j.jns.2016.02.026"
}

Živković, M., Kolaković, A., Stojković, L. S., Dinčić, E., Kostic, S., Alavantić, D.,& Stanković, A.. (2016). Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis. in Journal of the Neurological Sciences, 363, 29-32.
https://doi.org/10.1016/j.jns.2016.02.026

Živković M, Kolaković A, Stojković LS, Dinčić E, Kostic S, Alavantić D, Stanković A. Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis. in Journal of the Neurological Sciences. 2016;363:29-32.
doi:10.1016/j.jns.2016.02.026 .

Živković, Maja, Kolaković, Ana, Stojković, Ljiljana S., Dinčić, Evica, Kostic, Smiljana, Alavantić, Dragan, Stanković, Aleksandra, "Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis" in Journal of the Neurological Sciences, 363 (2016):29-32,
https://doi.org/10.1016/j.jns.2016.02.026 . .