TY  - JOUR
AU  - Cvetković, Zorica
AU  - Milošević, Maja
AU  - Cvetković, Bora
AU  - Masnikosa, Romana
AU  - Arsić, Aleksandra
AU  - Petrović, Snježana
AU  - Vučić, Vesna
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1489
AB  - Limited studies have been performed to associate abnormal phospholipid (PL) profile and disease activity in hematological malignancies, including non-Hodgkin lymphoma (NHL). The aim of his study was to evaluate the levels of plasma PL fractions in NHL patients, in response to chemotherapy. Forty non-treated patients with NHL and 25 healthy individuals were recruited. Blood samples from patients were taken before chemotherapy, after 3 cycles and after the end of the treatment, and PL fractions were resolved by one-dimensional thin-layer chromatography. To assess potential relationship between plasma PL profile and response to therapy, patients were divided according to clinical outcome in 3 groups: complete remission (CR), stable disease (SD) and progression (PG). Despite significant differences between NHL patients and healthy controls, no differences were found at baseline among patients divided according to clinical outcome. During and after chemotherapy important alterations in PL profile were observed. Levels of total PLs and all PL fractions decreased in patients with PG while in patients who responded to therapy (CR, SD) PLs significantly increased. Results of our study suggest that changes of total PLs and PL fractions during the therapy are associated with the effects of therapy and clinical outcome in patients with NHL. (C) 2017 Elsevier Ltd. All rights reserved.
T2  - Leukemia Research
T1  - Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients
VL  - 54
SP  - 39
EP  - 46
DO  - 10.1016/j.leukres.2017.01.004
ER  - 

@article{
author = "Cvetković, Zorica and Milošević, Maja and Cvetković, Bora and Masnikosa, Romana and Arsić, Aleksandra and Petrović, Snježana and Vučić, Vesna",
year = "2017",
abstract = "Limited studies have been performed to associate abnormal phospholipid (PL) profile and disease activity in hematological malignancies, including non-Hodgkin lymphoma (NHL). The aim of his study was to evaluate the levels of plasma PL fractions in NHL patients, in response to chemotherapy. Forty non-treated patients with NHL and 25 healthy individuals were recruited. Blood samples from patients were taken before chemotherapy, after 3 cycles and after the end of the treatment, and PL fractions were resolved by one-dimensional thin-layer chromatography. To assess potential relationship between plasma PL profile and response to therapy, patients were divided according to clinical outcome in 3 groups: complete remission (CR), stable disease (SD) and progression (PG). Despite significant differences between NHL patients and healthy controls, no differences were found at baseline among patients divided according to clinical outcome. During and after chemotherapy important alterations in PL profile were observed. Levels of total PLs and all PL fractions decreased in patients with PG while in patients who responded to therapy (CR, SD) PLs significantly increased. Results of our study suggest that changes of total PLs and PL fractions during the therapy are associated with the effects of therapy and clinical outcome in patients with NHL. (C) 2017 Elsevier Ltd. All rights reserved.",
journal = "Leukemia Research",
title = "Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients",
volume = "54",
pages = "39-46",
doi = "10.1016/j.leukres.2017.01.004"
}

Cvetković, Z., Milošević, M., Cvetković, B., Masnikosa, R., Arsić, A., Petrović, S.,& Vučić, V.. (2017). Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients. in Leukemia Research, 54, 39-46.
https://doi.org/10.1016/j.leukres.2017.01.004

Cvetković Z, Milošević M, Cvetković B, Masnikosa R, Arsić A, Petrović S, Vučić V. Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients. in Leukemia Research. 2017;54:39-46.
doi:10.1016/j.leukres.2017.01.004 .

Cvetković, Zorica, Milošević, Maja, Cvetković, Bora, Masnikosa, Romana, Arsić, Aleksandra, Petrović, Snježana, Vučić, Vesna, "Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients" in Leukemia Research, 54 (2017):39-46,
https://doi.org/10.1016/j.leukres.2017.01.004 . .

TY  - JOUR
AU  - Karadzic, Ivana
AU  - Vučić, Vesna
AU  - Jokanović, Vukoman R.
AU  - Debeljak-Martačić, Jasmina
AU  - Marković, Dejan
AU  - Petrović, Snježana
AU  - Glibetić, Marija
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/235
AB  - The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 350-357, 2015.
T2  - Journal of Biomedical Materials Research. Part A
T1  - Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells
VL  - 103
IS  - 1
SP  - 350
EP  - 357
DO  - 10.1002/jbm.a.35180
ER  - 

@article{
author = "Karadzic, Ivana and Vučić, Vesna and Jokanović, Vukoman R. and Debeljak-Martačić, Jasmina and Marković, Dejan and Petrović, Snježana and Glibetić, Marija",
year = "2015",
abstract = "The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 350-357, 2015.",
journal = "Journal of Biomedical Materials Research. Part A",
title = "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells",
volume = "103",
number = "1",
pages = "350-357",
doi = "10.1002/jbm.a.35180"
}

Karadzic, I., Vučić, V., Jokanović, V. R., Debeljak-Martačić, J., Marković, D., Petrović, S.,& Glibetić, M.. (2015). Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research. Part A, 103(1), 350-357.
https://doi.org/10.1002/jbm.a.35180

Karadzic I, Vučić V, Jokanović VR, Debeljak-Martačić J, Marković D, Petrović S, Glibetić M. Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research. Part A. 2015;103(1):350-357.
doi:10.1002/jbm.a.35180 .

Karadzic, Ivana, Vučić, Vesna, Jokanović, Vukoman R., Debeljak-Martačić, Jasmina, Marković, Dejan, Petrović, Snježana, Glibetić, Marija, "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells" in Journal of Biomedical Materials Research. Part A, 103, no. 1 (2015):350-357,
https://doi.org/10.1002/jbm.a.35180 . .

TY  - JOUR
AU  - Petrović, Snježana
AU  - Milošević, Maja
AU  - Ristic-Medic, Danijela
AU  - Velickovic, Natasa
AU  - Drakulić, Dunja R.
AU  - Grković, Ivana
AU  - Horvat, Anica
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/495
AB  - Our earlier studies found that in vitro estradiol modulates mitochondrial Ca2+ transport in discrete brain regions. The present study examined the role of estradiol receptors (ERs) in estradiol-induced inhibition of Ca2+ efflux from synaptosomal mitochondria isolated from rat caudate nuclei and brain stems. Radioactively labeled CaCl2 (0.6-0.75 mu Ci (CaCl2)-Ca-45) was used for Ca2+ transport monitoring. The results revealed that in the presence of ER antagonist 7 alpha, 17 beta-[9[(4,4,5,5,5-pentafluoropentyl) sulfinyl] nonyl] estra-1,3,5( 10)-triene-3,17-diol (ICI 182,780) (1 mu mol/L), the inhibitory effect of estradiol on mitochondrial Ca2+ efflux was more than 60% decreased, suggesting the involvement of ER in this mode of estradiol neuromodulatory action. When particular contributions of ER alpha and ER beta were tested, it was found that ER beta agonist 2,3-bis(4-hydroxy phenyl)-propionitrile (10 nmol/L) inhibited Ca2+ efflux more than 20%, while the inhibition with ER alpha agonist 4,4, 4-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol (10 nmol/L) was about 10%, both compared to the control. Both agonists demonstrated attenuation of Ca2+ efflux decrease in the presence of mitochondrial Na+/Ca2+ exchanger antagonist 7-chloro-5-(2-chlorophenyl)-1,5-dihyhdro-4,1-benzothiazepin-2(3H)-one (10 mu mol/L), showing interference with the inhibitory action of that agent. Our results strongly indicate ERs as the mediators of estradiol-induced mitochondrial Ca2+ efflux inhibition in rat caudate nucleus and brain stem synaptosomes.
T2  - Turkish Journal of Biology
T1  - Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem
VL  - 39
IS  - 2
SP  - 328
EP  - 334
DO  - 10.3906/biy-1408-62
ER  - 

@article{
author = "Petrović, Snježana and Milošević, Maja and Ristic-Medic, Danijela and Velickovic, Natasa and Drakulić, Dunja R. and Grković, Ivana and Horvat, Anica",
year = "2015",
abstract = "Our earlier studies found that in vitro estradiol modulates mitochondrial Ca2+ transport in discrete brain regions. The present study examined the role of estradiol receptors (ERs) in estradiol-induced inhibition of Ca2+ efflux from synaptosomal mitochondria isolated from rat caudate nuclei and brain stems. Radioactively labeled CaCl2 (0.6-0.75 mu Ci (CaCl2)-Ca-45) was used for Ca2+ transport monitoring. The results revealed that in the presence of ER antagonist 7 alpha, 17 beta-[9[(4,4,5,5,5-pentafluoropentyl) sulfinyl] nonyl] estra-1,3,5( 10)-triene-3,17-diol (ICI 182,780) (1 mu mol/L), the inhibitory effect of estradiol on mitochondrial Ca2+ efflux was more than 60% decreased, suggesting the involvement of ER in this mode of estradiol neuromodulatory action. When particular contributions of ER alpha and ER beta were tested, it was found that ER beta agonist 2,3-bis(4-hydroxy phenyl)-propionitrile (10 nmol/L) inhibited Ca2+ efflux more than 20%, while the inhibition with ER alpha agonist 4,4, 4-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol (10 nmol/L) was about 10%, both compared to the control. Both agonists demonstrated attenuation of Ca2+ efflux decrease in the presence of mitochondrial Na+/Ca2+ exchanger antagonist 7-chloro-5-(2-chlorophenyl)-1,5-dihyhdro-4,1-benzothiazepin-2(3H)-one (10 mu mol/L), showing interference with the inhibitory action of that agent. Our results strongly indicate ERs as the mediators of estradiol-induced mitochondrial Ca2+ efflux inhibition in rat caudate nucleus and brain stem synaptosomes.",
journal = "Turkish Journal of Biology",
title = "Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem",
volume = "39",
number = "2",
pages = "328-334",
doi = "10.3906/biy-1408-62"
}

Petrović, S., Milošević, M., Ristic-Medic, D., Velickovic, N., Drakulić, D. R., Grković, I.,& Horvat, A.. (2015). Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem. in Turkish Journal of Biology, 39(2), 328-334.
https://doi.org/10.3906/biy-1408-62

Petrović S, Milošević M, Ristic-Medic D, Velickovic N, Drakulić DR, Grković I, Horvat A. Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem. in Turkish Journal of Biology. 2015;39(2):328-334.
doi:10.3906/biy-1408-62 .

Petrović, Snježana, Milošević, Maja, Ristic-Medic, Danijela, Velickovic, Natasa, Drakulić, Dunja R., Grković, Ivana, Horvat, Anica, "Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem" in Turkish Journal of Biology, 39, no. 2 (2015):328-334,
https://doi.org/10.3906/biy-1408-62 . .

TY  - JOUR
AU  - Petrović, Snježana
AU  - Milošević, Maja
AU  - Drakulić, Dunja R.
AU  - Grković, Ivana
AU  - Stanojlović, Miloš R.
AU  - Mitrović, Nataša Lj.
AU  - Horvat, Anica
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5012
AB  - The aim of this study was to examine the rapid non-genomic effect of 17 beta-estradiol (E2) on Ca2+ transport in mitochondria isolated from the nerve terminals (synaptosomes) of caudate nuclei (NC) and brain stems (BS) of ovariectomised female rats. In physiological conditions no effect of E2 on Ca2+ influx into synaptosomal mitochondria through ruthenium red (RR)-sensitive uniporter was observed. However, in the presence of uncoupling agent carbonyl cyanide4-(trifluoromethoxy)phenylhydrazone (FCCP) (1 mu mol/l), pre-treatment with 0.5 nmol/l E2 protected mitochondrial membrane potential and consequently increased Ca2+ influx (2.3-fold in NC and 3.1-fold in BS). At the same time, 0.5 nmol/l E2 by increasing the affinity of mitochondrial Na+/Ca2+ exchanger for Na+ inhibited mitochondrial Ca2+ efflux in NC and BS by about 40%. Also, the specific binding of physiological E2 concentrations (0.1-10 nmol/l) to isolated synaptosomal mitochondria was detected. Using membrane impermeable E2 bound to bovine serum albumin and selective inhibitor of mitochondrial Na+/Ca2+ exchanger, we obtained that E2s action on mitochondrial Ca2+ efflux at least partially is due to the direct effects on the mitochondrial membrane and/or Na+/Ca2+ exchanger located in inner mitochondrial membrane. Our results implicate E2 as a modulator of Ca2+ concentration in mitochondrial matrix, and ultimately in the cytosol. Given the vital role of Ca2+ in regulation of total nerve cells activity, especially energy metabolism, neurotransmission and directing the cells toward survival or cell death, the effects on mitochondrial Ca2+ transport could be one of the important modes of E2 neuromodulatory action independent of the genome. (C) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.
T2  - Neuroscience
T1  - 17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem
VL  - 220
SP  - 32
EP  - 40
DO  - 10.1016/j.neuroscience.2012.06.040
ER  - 

@article{
author = "Petrović, Snježana and Milošević, Maja and Drakulić, Dunja R. and Grković, Ivana and Stanojlović, Miloš R. and Mitrović, Nataša Lj. and Horvat, Anica",
year = "2012",
abstract = "The aim of this study was to examine the rapid non-genomic effect of 17 beta-estradiol (E2) on Ca2+ transport in mitochondria isolated from the nerve terminals (synaptosomes) of caudate nuclei (NC) and brain stems (BS) of ovariectomised female rats. In physiological conditions no effect of E2 on Ca2+ influx into synaptosomal mitochondria through ruthenium red (RR)-sensitive uniporter was observed. However, in the presence of uncoupling agent carbonyl cyanide4-(trifluoromethoxy)phenylhydrazone (FCCP) (1 mu mol/l), pre-treatment with 0.5 nmol/l E2 protected mitochondrial membrane potential and consequently increased Ca2+ influx (2.3-fold in NC and 3.1-fold in BS). At the same time, 0.5 nmol/l E2 by increasing the affinity of mitochondrial Na+/Ca2+ exchanger for Na+ inhibited mitochondrial Ca2+ efflux in NC and BS by about 40%. Also, the specific binding of physiological E2 concentrations (0.1-10 nmol/l) to isolated synaptosomal mitochondria was detected. Using membrane impermeable E2 bound to bovine serum albumin and selective inhibitor of mitochondrial Na+/Ca2+ exchanger, we obtained that E2s action on mitochondrial Ca2+ efflux at least partially is due to the direct effects on the mitochondrial membrane and/or Na+/Ca2+ exchanger located in inner mitochondrial membrane. Our results implicate E2 as a modulator of Ca2+ concentration in mitochondrial matrix, and ultimately in the cytosol. Given the vital role of Ca2+ in regulation of total nerve cells activity, especially energy metabolism, neurotransmission and directing the cells toward survival or cell death, the effects on mitochondrial Ca2+ transport could be one of the important modes of E2 neuromodulatory action independent of the genome. (C) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.",
journal = "Neuroscience",
title = "17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem",
volume = "220",
pages = "32-40",
doi = "10.1016/j.neuroscience.2012.06.040"
}

Petrović, S., Milošević, M., Drakulić, D. R., Grković, I., Stanojlović, M. R., Mitrović, N. Lj.,& Horvat, A.. (2012). 17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem. in Neuroscience, 220, 32-40.
https://doi.org/10.1016/j.neuroscience.2012.06.040

Petrović S, Milošević M, Drakulić DR, Grković I, Stanojlović MR, Mitrović NL, Horvat A. 17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem. in Neuroscience. 2012;220:32-40.
doi:10.1016/j.neuroscience.2012.06.040 .

Petrović, Snježana, Milošević, Maja, Drakulić, Dunja R., Grković, Ivana, Stanojlović, Miloš R., Mitrović, Nataša Lj., Horvat, Anica, "17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem" in Neuroscience, 220 (2012):32-40,
https://doi.org/10.1016/j.neuroscience.2012.06.040 . .

TY  - CONF
AU  - Drakulić, Dunja R.
AU  - Grković, Ivana
AU  - Milošević, Maja
AU  - Petrović, Snježana
AU  - Stanojlović, Miloš R.
AU  - Mitrović, Nataša Lj.
AU  - Horvat, Anica
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9295
AB  - The present study was performed to investigate whether acute whole-body exposure of
female adult rats to low dose (0.5 Gy) of ionizing irradiation (IR) is sufficient to alter
ectonucleotidase enzyme activities in the brain. All measurements were done at time
points 1, 24 and 72h after irradiation. Neuronal synaptic plasma membranes (SPMs)
were isolated from whole brains and enzyme activities were determined by monitoring
ATP, ADP and AMP hydrolysis in vitro. Our results indicate that whole-body IR is
able to modulate investigated brain enzyme activities in a time-dependent manner.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
T1  - Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain
VL  - 1
SP  - 373
EP  - 375
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9295
ER  - 

@conference{
author = "Drakulić, Dunja R. and Grković, Ivana and Milošević, Maja and Petrović, Snježana and Stanojlović, Miloš R. and Mitrović, Nataša Lj. and Horvat, Anica",
year = "2012",
abstract = "The present study was performed to investigate whether acute whole-body exposure of
female adult rats to low dose (0.5 Gy) of ionizing irradiation (IR) is sufficient to alter
ectonucleotidase enzyme activities in the brain. All measurements were done at time
points 1, 24 and 72h after irradiation. Neuronal synaptic plasma membranes (SPMs)
were isolated from whole brains and enzyme activities were determined by monitoring
ATP, ADP and AMP hydrolysis in vitro. Our results indicate that whole-body IR is
able to modulate investigated brain enzyme activities in a time-dependent manner.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry",
title = "Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain",
volume = "1",
pages = "373-375",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9295"
}

Drakulić, D. R., Grković, I., Milošević, M., Petrović, S., Stanojlović, M. R., Mitrović, N. Lj.,& Horvat, A.. (2012). Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 373-375.
https://hdl.handle.net/21.15107/rcub_vinar_9295

Drakulić DR, Grković I, Milošević M, Petrović S, Stanojlović MR, Mitrović NL, Horvat A. Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry. 2012;1:373-375.
https://hdl.handle.net/21.15107/rcub_vinar_9295 .

Drakulić, Dunja R., Grković, Ivana, Milošević, Maja, Petrović, Snježana, Stanojlović, Miloš R., Mitrović, Nataša Lj., Horvat, Anica, "Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain" in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry, 1 (2012):373-375,
https://hdl.handle.net/21.15107/rcub_vinar_9295 .

TY  - JOUR
AU  - Stanojević, Ivana
AU  - Bjelobaba, Ivana
AU  - Nedeljković, Nadežda
AU  - Drakulić, Dunja R.
AU  - Petrović, Snježana
AU  - Stojiljković, Mirjana
AU  - Horvat, Anica
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4376
AB  - Ecto-5-nucleotidase (CD73; EC 3.1.3.5, e-5NT) is regarded as the key enzyme in the extracellular formation of adenosine, which acts as a neuromodulator and important trophic and homeostatic factor in the brain. In the present study, we have investigated e-5NT activity, kinetic properties concerning AMP hydrolysis and the enzyme protein abundance in the purified synaptic plasma membrane (SPM) preparations isolated from whole female rat brain at different ages. We observed pronounced increase in AMP hydrolyzing activity in SPM during maturation, with greatest increment between juvenile (15-day-old) and pre-pubertal (30-day-old) rats. Immunodetection of e-5NT protein in the SPM displayed the reverse pattern of expression, with the maximum relative abundance at juvenile and minimum relative abundance in the adult stage. Negative correlation between the enzyme activity and the enzyme protein abundance in the SPM indicates that e-5NT has additional roles in the synaptic compartment during postnatal brain development, other than those related to AMP hydrolysis. Determination of kinetic parameters, K(m) and V(max), suggested that the increase in the enzyme activity with maturation was entirely due to the increase in the enzyme catalytic efficiency (V(max)/K(m)). Finally, double immunofluorescence staining against e-5NT and presynaptic membrane marker syntaxin provided first direct evidence for the existence of this ecto-enzyme in the presynaptic compartment. The results of the study suggest that e-5NT may be a part of general scheme of brain development and synapse maturation and provide rationale for the previously reported inconsistencies between enzyme immunohistochemical and biochemical studies concerning localization of e-5NT in the brain. (C) 2011 ISDN. Published by Elsevier Ltd. All rights reserved.
T2  - International Journal of Developmental Neuroscience
T1  - Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes
VL  - 29
IS  - 4
SP  - 397
EP  - 403
DO  - 10.1016/j.ijdevneu.2011.03.003
ER  - 

@article{
author = "Stanojević, Ivana and Bjelobaba, Ivana and Nedeljković, Nadežda and Drakulić, Dunja R. and Petrović, Snježana and Stojiljković, Mirjana and Horvat, Anica",
year = "2011",
abstract = "Ecto-5-nucleotidase (CD73; EC 3.1.3.5, e-5NT) is regarded as the key enzyme in the extracellular formation of adenosine, which acts as a neuromodulator and important trophic and homeostatic factor in the brain. In the present study, we have investigated e-5NT activity, kinetic properties concerning AMP hydrolysis and the enzyme protein abundance in the purified synaptic plasma membrane (SPM) preparations isolated from whole female rat brain at different ages. We observed pronounced increase in AMP hydrolyzing activity in SPM during maturation, with greatest increment between juvenile (15-day-old) and pre-pubertal (30-day-old) rats. Immunodetection of e-5NT protein in the SPM displayed the reverse pattern of expression, with the maximum relative abundance at juvenile and minimum relative abundance in the adult stage. Negative correlation between the enzyme activity and the enzyme protein abundance in the SPM indicates that e-5NT has additional roles in the synaptic compartment during postnatal brain development, other than those related to AMP hydrolysis. Determination of kinetic parameters, K(m) and V(max), suggested that the increase in the enzyme activity with maturation was entirely due to the increase in the enzyme catalytic efficiency (V(max)/K(m)). Finally, double immunofluorescence staining against e-5NT and presynaptic membrane marker syntaxin provided first direct evidence for the existence of this ecto-enzyme in the presynaptic compartment. The results of the study suggest that e-5NT may be a part of general scheme of brain development and synapse maturation and provide rationale for the previously reported inconsistencies between enzyme immunohistochemical and biochemical studies concerning localization of e-5NT in the brain. (C) 2011 ISDN. Published by Elsevier Ltd. All rights reserved.",
journal = "International Journal of Developmental Neuroscience",
title = "Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes",
volume = "29",
number = "4",
pages = "397-403",
doi = "10.1016/j.ijdevneu.2011.03.003"
}

Stanojević, I., Bjelobaba, I., Nedeljković, N., Drakulić, D. R., Petrović, S., Stojiljković, M.,& Horvat, A.. (2011). Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes. in International Journal of Developmental Neuroscience, 29(4), 397-403.
https://doi.org/10.1016/j.ijdevneu.2011.03.003

Stanojević I, Bjelobaba I, Nedeljković N, Drakulić DR, Petrović S, Stojiljković M, Horvat A. Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes. in International Journal of Developmental Neuroscience. 2011;29(4):397-403.
doi:10.1016/j.ijdevneu.2011.03.003 .

Stanojević, Ivana, Bjelobaba, Ivana, Nedeljković, Nadežda, Drakulić, Dunja R., Petrović, Snježana, Stojiljković, Mirjana, Horvat, Anica, "Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes" in International Journal of Developmental Neuroscience, 29, no. 4 (2011):397-403,
https://doi.org/10.1016/j.ijdevneu.2011.03.003 . .

TY  - THES
AU  - Petrović, Snježana
PY  - 2011
UR  - http://www.vbs.rs/scripts/cobiss?command=DISPLAY&base=99999&rid=38201615&fmt=11&lani=sc
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7597
AB  - Kalcijum je element sveprisutan u živoj prirodi. S jedne strane on je gradivni element biljnih i životinjskih tkiva (ćelijski zid biljaka, ljušture mekušaca, skelet kičmenjaka), a sa druge strane, u svom jonskom obliku (Ca2+), ima važnu ulogu prenosioca signala u ćeliji i to od samog trenutka oplodnje i začetka novog života do odumiranja ćelija i smrti organizma. Preduslov signalne funkcije Ca2+ su oscilacije koncentracije Ca2+ u citosolu ([Ca2+]i ) koje se odvijaju u kontrolisanom opsegu od 20-100 nmol/l kod ćelija u mirovanju do > 500 nmol/l dok traje prenos signala (signalna transdukcija) (Exton, 1985; Colegrove i sar., 2000). Žive ćelije  su razvile brojne mehanizme za kontrolu [Ca2+]i koji su locirani kako na ćelijskoj membrani tako i na membranama ćelijskih organela, kao na primer endoplazmatičnog retikuluma i mitohondrija. Osim što omogućavaju brz porast koncentracije Ca2+ za potrebe prenosa signala, ovi mehanizmi transportom Ca2+ iz citosola, sprečavaju citotoksične efekte Ca2+ do kojih bi došlo usled produženog zadržavanja visokih koncentracija [Ca2+]i. Zato je jasno da bi svaki agens, koji bi imao uticaj na aktivnost bilo kog mehanizma za transport Ca2+, istovremeno značajno uticao na ukupnu ćelijsku Ca2+-homeostazu, a time i na celokupnu ćelijsku aktivnost i preživljavanje.
Ova doktorska teza, koja obuhvata nastavak ranije započetih istraživanja, bavi se izučavanjem uticaja steroidnog hormona estradiola (E2) na mitohondrijske Ca2+-transportne mehanizme u nervnom tkivu, tj. određenim moždanim regionima: hipokampusu, kaudalnom jedru i moždanom stablu. Ona je pokušaj da se dođe do novih saznanja u istraživanju uloge estradiola u regulaciji koncentracije Ca2+ u nervnim ćelijama, a na taj način i u modulaciji ukupne nervne aktivnosti.
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Specifično vezivanje i efekat estradiola na transport kalcijuma u mitohondrije izolovane iz nervnih završetaka regiona mozga pacova
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7597
ER  - 

@phdthesis{
author = "Petrović, Snježana",
year = "2011",
abstract = "Kalcijum je element sveprisutan u živoj prirodi. S jedne strane on je gradivni element biljnih i životinjskih tkiva (ćelijski zid biljaka, ljušture mekušaca, skelet kičmenjaka), a sa druge strane, u svom jonskom obliku (Ca2+), ima važnu ulogu prenosioca signala u ćeliji i to od samog trenutka oplodnje i začetka novog života do odumiranja ćelija i smrti organizma. Preduslov signalne funkcije Ca2+ su oscilacije koncentracije Ca2+ u citosolu ([Ca2+]i ) koje se odvijaju u kontrolisanom opsegu od 20-100 nmol/l kod ćelija u mirovanju do > 500 nmol/l dok traje prenos signala (signalna transdukcija) (Exton, 1985; Colegrove i sar., 2000). Žive ćelije  su razvile brojne mehanizme za kontrolu [Ca2+]i koji su locirani kako na ćelijskoj membrani tako i na membranama ćelijskih organela, kao na primer endoplazmatičnog retikuluma i mitohondrija. Osim što omogućavaju brz porast koncentracije Ca2+ za potrebe prenosa signala, ovi mehanizmi transportom Ca2+ iz citosola, sprečavaju citotoksične efekte Ca2+ do kojih bi došlo usled produženog zadržavanja visokih koncentracija [Ca2+]i. Zato je jasno da bi svaki agens, koji bi imao uticaj na aktivnost bilo kog mehanizma za transport Ca2+, istovremeno značajno uticao na ukupnu ćelijsku Ca2+-homeostazu, a time i na celokupnu ćelijsku aktivnost i preživljavanje.
Ova doktorska teza, koja obuhvata nastavak ranije započetih istraživanja, bavi se izučavanjem uticaja steroidnog hormona estradiola (E2) na mitohondrijske Ca2+-transportne mehanizme u nervnom tkivu, tj. određenim moždanim regionima: hipokampusu, kaudalnom jedru i moždanom stablu. Ona je pokušaj da se dođe do novih saznanja u istraživanju uloge estradiola u regulaciji koncentracije Ca2+ u nervnim ćelijama, a na taj način i u modulaciji ukupne nervne aktivnosti.",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Specifično vezivanje i efekat estradiola na transport kalcijuma u mitohondrije izolovane iz nervnih završetaka regiona mozga pacova",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7597"
}

Petrović, S.. (2011). Specifično vezivanje i efekat estradiola na transport kalcijuma u mitohondrije izolovane iz nervnih završetaka regiona mozga pacova. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_vinar_7597

Petrović S. Specifično vezivanje i efekat estradiola na transport kalcijuma u mitohondrije izolovane iz nervnih završetaka regiona mozga pacova. 2011;.
https://hdl.handle.net/21.15107/rcub_vinar_7597 .

Petrović, Snježana, "Specifično vezivanje i efekat estradiola na transport kalcijuma u mitohondrije izolovane iz nervnih završetaka regiona mozga pacova" (2011),
https://hdl.handle.net/21.15107/rcub_vinar_7597 .

TY  - JOUR
AU  - Horvat, Anica
AU  - Stanojević, Ivana
AU  - Drakulić, Dunja R.
AU  - Velickovic, Natasa
AU  - Petrović, Snježana
AU  - Milošević, Maja
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3941
AB  - The aim of the present study was to examine the effect of acute restraint stress on rat brain synaptosomal plasma membrane (SPM) ecto-nucleotidase activities at specific stages of postnatal development (15-, 30-, 60- and 90-day-old rats) by measuring the rates of ATP, ADP and AMP hydrolysis 1,24 and 72 h post-stress. At 1 h after stress NTPDase and ecto-5-nucleotidase activities were decreased in rats aged up to 60 days old. In adult rats elevated enzyme activities were detected, which indicated the existence of different short-term stress responses during development. A similar pattern of ATP and ADP hydrolysis changes as well as the ATP/ADP ratio in all developmental stages indicated that NTPDase3 was acutely affected after stress. The long-term effect of acute stress on NTPDase activity differed during postnatal development. In juvenile animals (15 days old) NTPDase activity was not altered. However, in later developmental stages (30 and 60 days old rats) NTPDase activity decreased and persisted for 72 h post-stress. In adult rats only ATP hydrolysis was decreased after 24 h, indicating that ecto-ATPase was affected by stress. Ecto-5-nucleotidase hydrolysing activity was decreased within 24 h in adult rats, while in 15- and 30-day old rats it decreased 72 h post-stress. At equivalent times in pubertal rats (60 days old) a slight activation of ecto-5-nucleotidase was detected. Our results highlight the developmental-dependence of brain ecto-nucleotidase susceptibility to acute stress and the likely existence of different mechanisms involved in time-dependent ecto-nucleotidase activity modulation following stress exposure. Clearly there are differences in the response of the purinergic system to acute restraint stress between young and adult rats. (C) 2009 ISDN. Published by Elsevier Ltd. All rights reserved.
T2  - International Journal of Developmental Neuroscience
T1  - Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development
VL  - 28
IS  - 2
SP  - 175
EP  - 182
DO  - 10.1016/j.ijdevneu.2009.11.005
ER  - 

@article{
author = "Horvat, Anica and Stanojević, Ivana and Drakulić, Dunja R. and Velickovic, Natasa and Petrović, Snježana and Milošević, Maja",
year = "2010",
abstract = "The aim of the present study was to examine the effect of acute restraint stress on rat brain synaptosomal plasma membrane (SPM) ecto-nucleotidase activities at specific stages of postnatal development (15-, 30-, 60- and 90-day-old rats) by measuring the rates of ATP, ADP and AMP hydrolysis 1,24 and 72 h post-stress. At 1 h after stress NTPDase and ecto-5-nucleotidase activities were decreased in rats aged up to 60 days old. In adult rats elevated enzyme activities were detected, which indicated the existence of different short-term stress responses during development. A similar pattern of ATP and ADP hydrolysis changes as well as the ATP/ADP ratio in all developmental stages indicated that NTPDase3 was acutely affected after stress. The long-term effect of acute stress on NTPDase activity differed during postnatal development. In juvenile animals (15 days old) NTPDase activity was not altered. However, in later developmental stages (30 and 60 days old rats) NTPDase activity decreased and persisted for 72 h post-stress. In adult rats only ATP hydrolysis was decreased after 24 h, indicating that ecto-ATPase was affected by stress. Ecto-5-nucleotidase hydrolysing activity was decreased within 24 h in adult rats, while in 15- and 30-day old rats it decreased 72 h post-stress. At equivalent times in pubertal rats (60 days old) a slight activation of ecto-5-nucleotidase was detected. Our results highlight the developmental-dependence of brain ecto-nucleotidase susceptibility to acute stress and the likely existence of different mechanisms involved in time-dependent ecto-nucleotidase activity modulation following stress exposure. Clearly there are differences in the response of the purinergic system to acute restraint stress between young and adult rats. (C) 2009 ISDN. Published by Elsevier Ltd. All rights reserved.",
journal = "International Journal of Developmental Neuroscience",
title = "Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development",
volume = "28",
number = "2",
pages = "175-182",
doi = "10.1016/j.ijdevneu.2009.11.005"
}

Horvat, A., Stanojević, I., Drakulić, D. R., Velickovic, N., Petrović, S.,& Milošević, M.. (2010). Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development. in International Journal of Developmental Neuroscience, 28(2), 175-182.
https://doi.org/10.1016/j.ijdevneu.2009.11.005

Horvat A, Stanojević I, Drakulić DR, Velickovic N, Petrović S, Milošević M. Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development. in International Journal of Developmental Neuroscience. 2010;28(2):175-182.
doi:10.1016/j.ijdevneu.2009.11.005 .

Horvat, Anica, Stanojević, Ivana, Drakulić, Dunja R., Velickovic, Natasa, Petrović, Snježana, Milošević, Maja, "Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development" in International Journal of Developmental Neuroscience, 28, no. 2 (2010):175-182,
https://doi.org/10.1016/j.ijdevneu.2009.11.005 . .

TY  - CONF
AU  - Milošević, Maja
AU  - Drakulić, Dunja R.
AU  - Petrović, Snježana
AU  - Stanojević, Ivana
AU  - Veličković, Nataša
AU  - Horvat, Anica
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9319
AB  - L-cysteine is used as effective oral chelating agent due to property of its sulfhydryl
group to bind heavy metal ions. The aim of this study was to investigate ability of
L-cysteine to prevent mercury (II) and cadmium (II) induced ecto-ATPase activity
inhibition of rat uterus plasma membranes. Results show that 10 mmol/l L-cysteine
have protective effect on enzyme activity in the presence of cadmium and mercury
ions.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9319
ER  - 

@conference{
author = "Milošević, Maja and Drakulić, Dunja R. and Petrović, Snježana and Stanojević, Ivana and Veličković, Nataša and Horvat, Anica",
year = "2010",
abstract = "L-cysteine is used as effective oral chelating agent due to property of its sulfhydryl
group to bind heavy metal ions. The aim of this study was to investigate ability of
L-cysteine to prevent mercury (II) and cadmium (II) induced ecto-ATPase activity
inhibition of rat uterus plasma membranes. Results show that 10 mmol/l L-cysteine
have protective effect on enzyme activity in the presence of cadmium and mercury
ions.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9319"
}

Milošević, M., Drakulić, D. R., Petrović, S., Stanojević, I., Veličković, N.,& Horvat, A.. (2010). L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
https://hdl.handle.net/21.15107/rcub_vinar_9319

Milošević M, Drakulić DR, Petrović S, Stanojević I, Veličković N, Horvat A. L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;.
https://hdl.handle.net/21.15107/rcub_vinar_9319 .

Milošević, Maja, Drakulić, Dunja R., Petrović, Snježana, Stanojević, Ivana, Veličković, Nataša, Horvat, Anica, "L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010),
https://hdl.handle.net/21.15107/rcub_vinar_9319 .

TY  - CONF
AU  - Petrović, Snježana
AU  - Milošević, Maja
AU  - Stanojević, Ivana
AU  - Drakulić, Dunja R.
AU  - Jovanović, N.
AU  - Veličković, Nataša
AU  - Horvat, Anica
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9321
AB  - In this study the Ca2+ ion flux modulation in the synaptosomal mitochondria
isolated from caudate nucleus (CN) of the ovariectomised rats was examined.
17 beta-estradiol (E2), E2-conjugated to bovine serum albumin (E-BSA), estradiol
receptor α (ERα) agonist 4,4',4''-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol
(PPT), ERβ agonist 2,3-bis(4-hydroxyphenyl)-propionitrile (DNP) and ERα/β
antagonist 7α,17β-[9[(4,4,5,5,5-pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-
triene-3,17-diol (ICI 182,780) were used. The Ca2+ efflux inhibition of about 27%
was detected in the presence of 0.5 nmol/l E2, and of about 20% in the case of
E-BSA. DNP (10 nmol/l) was as much potent Ca2+ efflux inhibitor as E2, while
PPT (10 nmol/l) hardly had any inhibitory effect (9% efflux decrease). When E2
binding to ERα and ERβ was prevented by 1 μmol/l ICI 182,780, the Ca2+ efflux
inhibition of about 15% was detected. Our results suggest that E2 prevents Ca2+
efflux from synaptosomal mitochondria due to ERβ activation rather than ERα.
The involvement of the external E2 binding site on the mitochondrial membrane
probably different from ERα/β should not be excluded because of Ca2+ efflux
inhibition detected in the presence of E-BSA and ICI 182,780. The Ca2+ efflux
modulation could be the mechanism through which E2 exerts its neuromodulatory
role in specific brain structures.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9321
ER  - 

@conference{
author = "Petrović, Snježana and Milošević, Maja and Stanojević, Ivana and Drakulić, Dunja R. and Jovanović, N. and Veličković, Nataša and Horvat, Anica",
year = "2010",
abstract = "In this study the Ca2+ ion flux modulation in the synaptosomal mitochondria
isolated from caudate nucleus (CN) of the ovariectomised rats was examined.
17 beta-estradiol (E2), E2-conjugated to bovine serum albumin (E-BSA), estradiol
receptor α (ERα) agonist 4,4',4''-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol
(PPT), ERβ agonist 2,3-bis(4-hydroxyphenyl)-propionitrile (DNP) and ERα/β
antagonist 7α,17β-[9[(4,4,5,5,5-pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-
triene-3,17-diol (ICI 182,780) were used. The Ca2+ efflux inhibition of about 27%
was detected in the presence of 0.5 nmol/l E2, and of about 20% in the case of
E-BSA. DNP (10 nmol/l) was as much potent Ca2+ efflux inhibitor as E2, while
PPT (10 nmol/l) hardly had any inhibitory effect (9% efflux decrease). When E2
binding to ERα and ERβ was prevented by 1 μmol/l ICI 182,780, the Ca2+ efflux
inhibition of about 15% was detected. Our results suggest that E2 prevents Ca2+
efflux from synaptosomal mitochondria due to ERβ activation rather than ERα.
The involvement of the external E2 binding site on the mitochondrial membrane
probably different from ERα/β should not be excluded because of Ca2+ efflux
inhibition detected in the presence of E-BSA and ICI 182,780. The Ca2+ efflux
modulation could be the mechanism through which E2 exerts its neuromodulatory
role in specific brain structures.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9321"
}

Petrović, S., Milošević, M., Stanojević, I., Drakulić, D. R., Jovanović, N., Veličković, N.,& Horvat, A.. (2010). Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
https://hdl.handle.net/21.15107/rcub_vinar_9321

Petrović S, Milošević M, Stanojević I, Drakulić DR, Jovanović N, Veličković N, Horvat A. Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;.
https://hdl.handle.net/21.15107/rcub_vinar_9321 .

Petrović, Snježana, Milošević, Maja, Stanojević, Ivana, Drakulić, Dunja R., Jovanović, N., Veličković, Nataša, Horvat, Anica, "Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010),
https://hdl.handle.net/21.15107/rcub_vinar_9321 .

TY  - JOUR
AU  - Petrović, Snježana
AU  - Milošević, Maja
AU  - Stanojević, Ivana
AU  - Velickovic, Natasa
AU  - Drakulić, Dunja R.
AU  - Horvat, Anica
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3749
AB  - The role of membrane-bound estradiol in modulation of mitochondrial Ca2+ flux in nerve endings isolated from rat brain stem was examined. Physiological concentrations of 17 beta-estradiol bind specifically to isolated mitochondria (Bmax 33.8 +/- 2.5 fmoles estradiol/mg of protein, Km 0.185 +/- 0.006 nmoles/l free estradiol). At concentrations ranging from 1 x 10-10 to 2 x 10-9 moles/l, estradiol significantly (by 23-28%) decreases mitochondrial Na-dependent calcium efflux. Decreased calcium efflux was associated with increased affinity of the Na+/Ca2+ exchanger for Na+ and decreased capacity of the exchanger to extrude Ca2+. Calcium ion efflux modulation and mitochondrial ion retention may be the way that 17 beta-estradiol exerts its role in nerve cell homeostasis.
T2  - Archives of Biological Sciences
T1  - Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol
VL  - 61
IS  - 2
SP  - 171
EP  - 177
DO  - 10.2298/ABS0902171P
ER  - 

@article{
author = "Petrović, Snježana and Milošević, Maja and Stanojević, Ivana and Velickovic, Natasa and Drakulić, Dunja R. and Horvat, Anica",
year = "2009",
abstract = "The role of membrane-bound estradiol in modulation of mitochondrial Ca2+ flux in nerve endings isolated from rat brain stem was examined. Physiological concentrations of 17 beta-estradiol bind specifically to isolated mitochondria (Bmax 33.8 +/- 2.5 fmoles estradiol/mg of protein, Km 0.185 +/- 0.006 nmoles/l free estradiol). At concentrations ranging from 1 x 10-10 to 2 x 10-9 moles/l, estradiol significantly (by 23-28%) decreases mitochondrial Na-dependent calcium efflux. Decreased calcium efflux was associated with increased affinity of the Na+/Ca2+ exchanger for Na+ and decreased capacity of the exchanger to extrude Ca2+. Calcium ion efflux modulation and mitochondrial ion retention may be the way that 17 beta-estradiol exerts its role in nerve cell homeostasis.",
journal = "Archives of Biological Sciences",
title = "Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol",
volume = "61",
number = "2",
pages = "171-177",
doi = "10.2298/ABS0902171P"
}

Petrović, S., Milošević, M., Stanojević, I., Velickovic, N., Drakulić, D. R.,& Horvat, A.. (2009). Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol. in Archives of Biological Sciences, 61(2), 171-177.
https://doi.org/10.2298/ABS0902171P

Petrović S, Milošević M, Stanojević I, Velickovic N, Drakulić DR, Horvat A. Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol. in Archives of Biological Sciences. 2009;61(2):171-177.
doi:10.2298/ABS0902171P .

Petrović, Snježana, Milošević, Maja, Stanojević, Ivana, Velickovic, Natasa, Drakulić, Dunja R., Horvat, Anica, "Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol" in Archives of Biological Sciences, 61, no. 2 (2009):171-177,
https://doi.org/10.2298/ABS0902171P . .

TY  - CONF
AU  - Petrović, Snježana
AU  - Milošević, Maja
AU  - Horvat, A.
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9498
AB  - In the present study, the flux of Ca2+ ions in the synaptosomal mitochondrial membrane isolated from the whole brain and hippocampus of chronically ovariectomized female rats was examined. Under basal conditions no significant difference was found. Addition of estradiol (0.5 nmol/l) in the preincubation mixture decreased significantly (25%) Na-dependent Ca2+ efflux in mitochondria from both sources which may be the way that it exerts its role in nerve cell homeostasis.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - Flux of Ca2+ ions in the synaptosomal mitochondrial membrane
VL  - 1
SP  - 371
EP  - 373
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9498
ER  - 

@conference{
author = "Petrović, Snježana and Milošević, Maja and Horvat, A.",
year = "2004",
abstract = "In the present study, the flux of Ca2+ ions in the synaptosomal mitochondrial membrane isolated from the whole brain and hippocampus of chronically ovariectomized female rats was examined. Under basal conditions no significant difference was found. Addition of estradiol (0.5 nmol/l) in the preincubation mixture decreased significantly (25%) Na-dependent Ca2+ efflux in mitochondria from both sources which may be the way that it exerts its role in nerve cell homeostasis.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Flux of Ca2+ ions in the synaptosomal mitochondrial membrane",
volume = "1",
pages = "371-373",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9498"
}

Petrović, S., Milošević, M.,& Horvat, A.. (2004). Flux of Ca2+ ions in the synaptosomal mitochondrial membrane. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 371-373.
https://hdl.handle.net/21.15107/rcub_vinar_9498

Petrović S, Milošević M, Horvat A. Flux of Ca2+ ions in the synaptosomal mitochondrial membrane. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:371-373.
https://hdl.handle.net/21.15107/rcub_vinar_9498 .

Petrović, Snježana, Milošević, Maja, Horvat, A., "Flux of Ca2+ ions in the synaptosomal mitochondrial membrane" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):371-373,
https://hdl.handle.net/21.15107/rcub_vinar_9498 .