TY  - CONF
AU  - Masnikosa, Romana
AU  - Pirić, David
AU  - Cvetković, Zorica
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12413
AB  - Both cancer and inflammation are almost invariably accompanied by lipidome dysregulation. Hence, various lipid species have been reported as candidate markers for many solid tumours1-3. However, neither the global lipidome nor sub-lipidome of inflammatory pathways in Non-Hodgkin lymphoma (NHL) has been studied. In order to fill this gap and shed light on the inflammatory pathways accompanying NHL, we designed a targeted liquid chromatography – Multiple Reaction Monitoring of bioactive lipids/lipid mediators in plasma of female patients with Diffuse Large B-cell Lymphoma (DLBCL), the most often type of NHL. We chose to quantify lipids known or hypothesized to be involved in inflammation and cancer progression along with their membrane precursors. In a pilot study encompassing plasma samples from 17 DLBCL patients and 21 BMI-matched controls, we analysed levels of pro-inlammatory arachidonic acid (AA)-derived oxylipins, focusing on lipoxygenase (LOX) and cytochrome P450 monooxygenase products: hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids; several AA-containing phospholipids (PLs); specificlysophospholipid subclasses; sphingomyelins (SMs), sphingosine 1-phosphate (S1P) and polyunsaturated fatty acids. Data were subjected to classical statistics and multivariate unsupervised and supervised machine learning (ML) algorithms. The DLBCL status was profoundly associated with altered S1P, SM 34:1, SM 36:1 and phosphatidylinositol PI 34:1 abundance. On the other hand, eicosanoids 12(S)-HETE, 15(S)-HETE and thromboxane B2 were major lipid species dis criminating between DLBCL and healthy status, as well as lysophosphatidylinositol LPI 20:4. The correlations between lipid species varied considerably between the cancer and controls, reflecting significant changes in lipid metabolic and/or signalling pathways, particularly those within LOX pathway and cell membrane PL remodelling. We suggest S1P, SM 36:1, SM 34:1 and PI 34:1 may beviewed as lipid signatures of DLBCL. Furthermore, these four lipid species could serve as a basis for the prospective validation in larger DLBCL/NHL clinical studies. As far as we know, this is the first plasma lipid profiling in DLBCL/NHL and, as such, brings new knowledge on the metabolic basis of inflammation in this cancer. The added value of our plasma lipid profiling in DLBCL is a deeper understanding of particulate lipid dysregulations in this tumour.
PB  - Kragujevac : Faculty of Science, University of Kragujevac
C3  - SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts
T1  - Plasma Profile of Inflamatory Mediators in NHL Patients
SP  - OP5
EP  - OP5
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12413
ER  - 

@conference{
author = "Masnikosa, Romana and Pirić, David and Cvetković, Zorica",
year = "2023",
abstract = "Both cancer and inflammation are almost invariably accompanied by lipidome dysregulation. Hence, various lipid species have been reported as candidate markers for many solid tumours1-3. However, neither the global lipidome nor sub-lipidome of inflammatory pathways in Non-Hodgkin lymphoma (NHL) has been studied. In order to fill this gap and shed light on the inflammatory pathways accompanying NHL, we designed a targeted liquid chromatography – Multiple Reaction Monitoring of bioactive lipids/lipid mediators in plasma of female patients with Diffuse Large B-cell Lymphoma (DLBCL), the most often type of NHL. We chose to quantify lipids known or hypothesized to be involved in inflammation and cancer progression along with their membrane precursors. In a pilot study encompassing plasma samples from 17 DLBCL patients and 21 BMI-matched controls, we analysed levels of pro-inlammatory arachidonic acid (AA)-derived oxylipins, focusing on lipoxygenase (LOX) and cytochrome P450 monooxygenase products: hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids; several AA-containing phospholipids (PLs); specificlysophospholipid subclasses; sphingomyelins (SMs), sphingosine 1-phosphate (S1P) and polyunsaturated fatty acids. Data were subjected to classical statistics and multivariate unsupervised and supervised machine learning (ML) algorithms. The DLBCL status was profoundly associated with altered S1P, SM 34:1, SM 36:1 and phosphatidylinositol PI 34:1 abundance. On the other hand, eicosanoids 12(S)-HETE, 15(S)-HETE and thromboxane B2 were major lipid species dis criminating between DLBCL and healthy status, as well as lysophosphatidylinositol LPI 20:4. The correlations between lipid species varied considerably between the cancer and controls, reflecting significant changes in lipid metabolic and/or signalling pathways, particularly those within LOX pathway and cell membrane PL remodelling. We suggest S1P, SM 36:1, SM 34:1 and PI 34:1 may beviewed as lipid signatures of DLBCL. Furthermore, these four lipid species could serve as a basis for the prospective validation in larger DLBCL/NHL clinical studies. As far as we know, this is the first plasma lipid profiling in DLBCL/NHL and, as such, brings new knowledge on the metabolic basis of inflammation in this cancer. The added value of our plasma lipid profiling in DLBCL is a deeper understanding of particulate lipid dysregulations in this tumour.",
publisher = "Kragujevac : Faculty of Science, University of Kragujevac",
journal = "SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts",
title = "Plasma Profile of Inflamatory Mediators in NHL Patients",
pages = "OP5-OP5",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12413"
}

Masnikosa, R., Pirić, D.,& Cvetković, Z.. (2023). Plasma Profile of Inflamatory Mediators in NHL Patients. in SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts
Kragujevac : Faculty of Science, University of Kragujevac., OP5-OP5.
https://hdl.handle.net/21.15107/rcub_vinar_12413

Masnikosa R, Pirić D, Cvetković Z. Plasma Profile of Inflamatory Mediators in NHL Patients. in SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts. 2023;:OP5-OP5.
https://hdl.handle.net/21.15107/rcub_vinar_12413 .

Masnikosa, Romana, Pirić, David, Cvetković, Zorica, "Plasma Profile of Inflamatory Mediators in NHL Patients" in SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts (2023):OP5-OP5,
https://hdl.handle.net/21.15107/rcub_vinar_12413 .

TY  - CONF
AU  - Masnikosa, Romana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12411
UR  - https://web.archive.org/web/20231225134750/https://indico.jinr.ru/event/4111/contributions/23661/
UR  - https://web.archive.org/web/20240112092428/https://indico.jinr.ru/event/4111/
AB  - In recent years, due to significant advancements in mass spectrometry, lipidomics has emerged as a fast growing scientific field that provides deep insights into complex changes in lipidome of human cells and tissues. Human blood is a self-regenerating lipid-rich biological fluid, a tissue that is easily collected in hospitals. Blood/plasma is rich in lipids and related metabolites, and its lipid composition (LIPIDOME) reflects diverse aspects of lipid metabolism and may give us insight into general human physiology in health and disease. Plasma lipidome is a tightly regulated and precisely defined constellation of lipid molecules and disturbances in the plasma lipidome occur in many diseases such as cardiovascular diseases and cancer, but also in conditions that are not directly linked to lipid metabolism. Hence, plasma lipidomics is an emerging tool in an array of clinical diagnostics. The most sought-after goals in the lipidomics community are to identify disease biomarkers, monitor a clinical treatment or confirm a biological hypothesis on a causality between a disease onset/progression and lipid profile. A recommended plasma lipidomics workflow consists of: preanalytics, analytics and post analytics, including study design, research hypotheses, sample collection, demographics data collection, lipid extraction, quality control, liquid chromatography and mass spectrometry, raw data processing, lipid annotation/identification, normalization, lipid quantitation, databases, data sharing and data analysis. The topic of this presentation is data analysis so I will put an accent to it. Lipidomics community nowadays uses dozens of biostatistical tools, artificial intelligence (AI), machine learning (ML) algorithms and chemometrics for data analysis, depending on size of datasets, data structure and the expertise of data scientists involved. Smart analysis of lipidomic data (provided these are of good quality) is of paramount importance for identifying potential biomarkers and understanding disease mechanisms. To accomplish this, they use, besides classical statistics-between groups comparisons, principal component analysis (PCA), orthogonal projections to latent structures discriminant analysis (OPLS-DA), partial least square analysis (PLS) and correlation analysis, all accompanied by various data pattern recognition and data visualization tools. Less often used are ML algorithms such as random forests (RF) and support vector machines (SVM), with only few applying deep learning and neural networks. Finally, to extract biological knowledge, i.e. metabolic pathways affected by a disease or applied treatment, data are subjected to different enrichment analyses, network analyses and pathway analysis. Life scientists working outside clinical setting, such as those investigating neurodegenerative changes in brain will also benefit from applying lipidomics to their model systems.
C3  - International workshop “Modern information technologies in biology and medicine”
T1  - Extracting biological meaning from lipidomics data through biostatistics, machine learning and pathway analysis
SP  - contribution ID: 10
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12411
ER  - 

@conference{
author = "Masnikosa, Romana",
year = "2023",
abstract = "In recent years, due to significant advancements in mass spectrometry, lipidomics has emerged as a fast growing scientific field that provides deep insights into complex changes in lipidome of human cells and tissues. Human blood is a self-regenerating lipid-rich biological fluid, a tissue that is easily collected in hospitals. Blood/plasma is rich in lipids and related metabolites, and its lipid composition (LIPIDOME) reflects diverse aspects of lipid metabolism and may give us insight into general human physiology in health and disease. Plasma lipidome is a tightly regulated and precisely defined constellation of lipid molecules and disturbances in the plasma lipidome occur in many diseases such as cardiovascular diseases and cancer, but also in conditions that are not directly linked to lipid metabolism. Hence, plasma lipidomics is an emerging tool in an array of clinical diagnostics. The most sought-after goals in the lipidomics community are to identify disease biomarkers, monitor a clinical treatment or confirm a biological hypothesis on a causality between a disease onset/progression and lipid profile. A recommended plasma lipidomics workflow consists of: preanalytics, analytics and post analytics, including study design, research hypotheses, sample collection, demographics data collection, lipid extraction, quality control, liquid chromatography and mass spectrometry, raw data processing, lipid annotation/identification, normalization, lipid quantitation, databases, data sharing and data analysis. The topic of this presentation is data analysis so I will put an accent to it. Lipidomics community nowadays uses dozens of biostatistical tools, artificial intelligence (AI), machine learning (ML) algorithms and chemometrics for data analysis, depending on size of datasets, data structure and the expertise of data scientists involved. Smart analysis of lipidomic data (provided these are of good quality) is of paramount importance for identifying potential biomarkers and understanding disease mechanisms. To accomplish this, they use, besides classical statistics-between groups comparisons, principal component analysis (PCA), orthogonal projections to latent structures discriminant analysis (OPLS-DA), partial least square analysis (PLS) and correlation analysis, all accompanied by various data pattern recognition and data visualization tools. Less often used are ML algorithms such as random forests (RF) and support vector machines (SVM), with only few applying deep learning and neural networks. Finally, to extract biological knowledge, i.e. metabolic pathways affected by a disease or applied treatment, data are subjected to different enrichment analyses, network analyses and pathway analysis. Life scientists working outside clinical setting, such as those investigating neurodegenerative changes in brain will also benefit from applying lipidomics to their model systems.",
journal = "International workshop “Modern information technologies in biology and medicine”",
title = "Extracting biological meaning from lipidomics data through biostatistics, machine learning and pathway analysis",
pages = "contribution ID: 10",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12411"
}

Masnikosa, R.. (2023). Extracting biological meaning from lipidomics data through biostatistics, machine learning and pathway analysis. in International workshop “Modern information technologies in biology and medicine”, contribution ID: 10.
https://hdl.handle.net/21.15107/rcub_vinar_12411

Masnikosa R. Extracting biological meaning from lipidomics data through biostatistics, machine learning and pathway analysis. in International workshop “Modern information technologies in biology and medicine”. 2023;:contribution ID: 10.
https://hdl.handle.net/21.15107/rcub_vinar_12411 .

Masnikosa, Romana, "Extracting biological meaning from lipidomics data through biostatistics, machine learning and pathway analysis" in International workshop “Modern information technologies in biology and medicine” (2023):contribution ID: 10,
https://hdl.handle.net/21.15107/rcub_vinar_12411 .

TY  - CONF
AU  - Masnikosa, Romana
AU  - Pirić, David
AU  - Cvetković, Zorica
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12257
AB  - Both cancer and inflammation are almost invariably accompanied by lipidome dysregulation. Hence, various lipid species have been reported as candidate markers for many solid tumours 1-3 .However, neitherthe globallipidome norsub-lipidome ofinflammatory pathways in Non-Hodgkin lymphoma (NHL) has been studied. In order to fill this gap and shed light on the inflammatory pathways accompanying NHL, we designed a targeted liquid chromatography – Multiple Reaction Monitoring of bioactive lipids/lipid mediators in plasma of female patients with Diffuse Large B-cell Lymphoma (DLBCL), the most often type of NHL. We chose to quantify lipids known or hypothesized to be involved in inflammation and cancer progression along with theirmembrane precursors. In a pilot study encompassing plasma samples from 17 DLBCL patients and 21 BMI-matched controls, we analysed levels of pro-inlammatory arachidonic acid (AA)-derived oxylipins, focusing on lipoxygenase (LOX) and cytochrome P450 monooxygenase products: hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids; several AA-containing phospholipids (PLs); specificlysophospholipid subclasses; sphingomyelins (SMs), sphingosine 1-phosphate (S1P) and polyunsaturated fatty acids. Data were subjected to classical statistics and multivariate unsupervised and supervised machine learning (ML) algorithms. The DLBCL status was profoundly associated with altered S1P, SM 34:1, SM 36:1 and phosphatidylinositol PI 34:1 abundance. On the other hand, eicosanoids 12(S)-HETE, 15(S)-HETE and thromboxane B2 were major lipid species discriminating between DLBCL and healthy status, as well as lysophosphatidylinositol LPI 20:4. The correlations between lipid species varied considerably between the cancer and controls, reflecting significant changes in lipid metabolic and/or signalling pathways, particularly those within LOX pathway and cell membrane PL remodelling. We suggest S1P, SM 36:1, SM 34:1 and PI 34:1 may beviewed as lipid signatures of DLBCL. Furthermore, these four lipid species could serve asa basis for the prospective validation in larger DLBCL/NHL clinical studies. As far as we know, this is the first plasma lipid profiling in DLBCL/NHL and, as such, brings new knowledge on the metabolic basis of inflammation in this cancer. The added value of our plasma lipid profiling in DLBCL is a deeper understanding of particulate lipid dysregulations in this tumour
PB  - Belgrade : Faculty of Science, University of Kragujevac
C3  - SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts
T1  - Plasma Profile of Inflamatory Mediators in NHL Patients
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12257
ER  - 

@conference{
author = "Masnikosa, Romana and Pirić, David and Cvetković, Zorica",
year = "2023",
abstract = "Both cancer and inflammation are almost invariably accompanied by lipidome dysregulation. Hence, various lipid species have been reported as candidate markers for many solid tumours 1-3 .However, neitherthe globallipidome norsub-lipidome ofinflammatory pathways in Non-Hodgkin lymphoma (NHL) has been studied. In order to fill this gap and shed light on the inflammatory pathways accompanying NHL, we designed a targeted liquid chromatography – Multiple Reaction Monitoring of bioactive lipids/lipid mediators in plasma of female patients with Diffuse Large B-cell Lymphoma (DLBCL), the most often type of NHL. We chose to quantify lipids known or hypothesized to be involved in inflammation and cancer progression along with theirmembrane precursors. In a pilot study encompassing plasma samples from 17 DLBCL patients and 21 BMI-matched controls, we analysed levels of pro-inlammatory arachidonic acid (AA)-derived oxylipins, focusing on lipoxygenase (LOX) and cytochrome P450 monooxygenase products: hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids; several AA-containing phospholipids (PLs); specificlysophospholipid subclasses; sphingomyelins (SMs), sphingosine 1-phosphate (S1P) and polyunsaturated fatty acids. Data were subjected to classical statistics and multivariate unsupervised and supervised machine learning (ML) algorithms. The DLBCL status was profoundly associated with altered S1P, SM 34:1, SM 36:1 and phosphatidylinositol PI 34:1 abundance. On the other hand, eicosanoids 12(S)-HETE, 15(S)-HETE and thromboxane B2 were major lipid species discriminating between DLBCL and healthy status, as well as lysophosphatidylinositol LPI 20:4. The correlations between lipid species varied considerably between the cancer and controls, reflecting significant changes in lipid metabolic and/or signalling pathways, particularly those within LOX pathway and cell membrane PL remodelling. We suggest S1P, SM 36:1, SM 34:1 and PI 34:1 may beviewed as lipid signatures of DLBCL. Furthermore, these four lipid species could serve asa basis for the prospective validation in larger DLBCL/NHL clinical studies. As far as we know, this is the first plasma lipid profiling in DLBCL/NHL and, as such, brings new knowledge on the metabolic basis of inflammation in this cancer. The added value of our plasma lipid profiling in DLBCL is a deeper understanding of particulate lipid dysregulations in this tumour",
publisher = "Belgrade : Faculty of Science, University of Kragujevac",
journal = "SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts",
title = "Plasma Profile of Inflamatory Mediators in NHL Patients",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12257"
}

Masnikosa, R., Pirić, D.,& Cvetković, Z.. (2023). Plasma Profile of Inflamatory Mediators in NHL Patients. in SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts
Belgrade : Faculty of Science, University of Kragujevac..
https://hdl.handle.net/21.15107/rcub_vinar_12257

Masnikosa R, Pirić D, Cvetković Z. Plasma Profile of Inflamatory Mediators in NHL Patients. in SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_12257 .

Masnikosa, Romana, Pirić, David, Cvetković, Zorica, "Plasma Profile of Inflamatory Mediators in NHL Patients" in SePA : VI Symposium of a Serbian proteomic society: „Discussion and Application of New Methods of Proteomics“ : Book of abstracts (2023),
https://hdl.handle.net/21.15107/rcub_vinar_12257 .

TY  - JOUR
AU  - Masnikosa, Romana
AU  - Pirić, David
AU  - Post, Julia Maria
AU  - Cvetković, Zorica
AU  - Petrović, Snježana
AU  - Paunović, Marija
AU  - Vučić, Vesna
AU  - Bindila, Laura
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11437
AB  - Lipidome dysregulation is a hallmark of cancer and inflammation. The global plasma lipidome and sub-lipidome of inflammatory pathways have not been reported in diffuse large B-cell lymphoma (DLBCL). In a pilot study of plasma lipid variation in female DLBCL patients and BMI-matched disease-free controls, we performed targeted lipidomics using LC-MRM to quantify lipid mediators of inflammation and immunity, and those known or hypothesised to be involved in cancer progression: sphingolipids, resolvin D1, arachidonic acid (AA)-derived oxylipins, such as hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids, along with their membrane structural precursors. We report on the role of the eicosanoids in the separation of DLBCL from controls, along with lysophosphatidylinositol LPI 20:4, implying notable changes in lipid metabolic and/or signalling pathways, particularly pertaining to AA lipoxygenase pathway and glycerophospholipid remodelling in the cell membrane. We suggest here the set of S1P, SM 36:1, SM 34:1 and PI 34:1 as DLBCL lipid signatures which could serve as a basis for the prospective validation in larger DLBCL cohorts. Additionally, untargeted lipidomics indicates a substantial change in the overall lipid metabolism in DLBCL. The plasma lipid profiling of DLBCL patients helps to better understand the specific lipid dysregulations and pathways in this cancer.
T2  - Cancers
T1  - Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study
VL  - 15
IS  - 14
SP  - 3653
DO  - 10.3390/cancers15143653
ER  - 

@article{
author = "Masnikosa, Romana and Pirić, David and Post, Julia Maria and Cvetković, Zorica and Petrović, Snježana and Paunović, Marija and Vučić, Vesna and Bindila, Laura",
year = "2023",
abstract = "Lipidome dysregulation is a hallmark of cancer and inflammation. The global plasma lipidome and sub-lipidome of inflammatory pathways have not been reported in diffuse large B-cell lymphoma (DLBCL). In a pilot study of plasma lipid variation in female DLBCL patients and BMI-matched disease-free controls, we performed targeted lipidomics using LC-MRM to quantify lipid mediators of inflammation and immunity, and those known or hypothesised to be involved in cancer progression: sphingolipids, resolvin D1, arachidonic acid (AA)-derived oxylipins, such as hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids, along with their membrane structural precursors. We report on the role of the eicosanoids in the separation of DLBCL from controls, along with lysophosphatidylinositol LPI 20:4, implying notable changes in lipid metabolic and/or signalling pathways, particularly pertaining to AA lipoxygenase pathway and glycerophospholipid remodelling in the cell membrane. We suggest here the set of S1P, SM 36:1, SM 34:1 and PI 34:1 as DLBCL lipid signatures which could serve as a basis for the prospective validation in larger DLBCL cohorts. Additionally, untargeted lipidomics indicates a substantial change in the overall lipid metabolism in DLBCL. The plasma lipid profiling of DLBCL patients helps to better understand the specific lipid dysregulations and pathways in this cancer.",
journal = "Cancers",
title = "Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study",
volume = "15",
number = "14",
pages = "3653",
doi = "10.3390/cancers15143653"
}

Masnikosa, R., Pirić, D., Post, J. M., Cvetković, Z., Petrović, S., Paunović, M., Vučić, V.,& Bindila, L.. (2023). Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study. in Cancers, 15(14), 3653.
https://doi.org/10.3390/cancers15143653

Masnikosa R, Pirić D, Post JM, Cvetković Z, Petrović S, Paunović M, Vučić V, Bindila L. Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study. in Cancers. 2023;15(14):3653.
doi:10.3390/cancers15143653 .

Masnikosa, Romana, Pirić, David, Post, Julia Maria, Cvetković, Zorica, Petrović, Snježana, Paunović, Marija, Vučić, Vesna, Bindila, Laura, "Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study" in Cancers, 15, no. 14 (2023):3653,
https://doi.org/10.3390/cancers15143653 . .

TY  - JOUR
AU  - Kolesnikova, I. A.
AU  - Lalkovičova, M.
AU  - Severyukhin, Yu. S.
AU  - Golikova, K. N.
AU  - Utina, D. M.
AU  - Pronskikh, E. V.
AU  - Despotović, Sanja Z.
AU  - Gaevsky, V. N.
AU  - Pirić, David
AU  - Masnikosa, Romana
AU  - Budennaya, N. N.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11224
AB  - We designed a study with the objective to determine the long-term radiation effects of gamma rays, originating from a single shot of Co60 at a dose of 2 Gy on the 7-month-old male mice of the ICR line in 30 days after the irradiation. The aim of this study was to characterize the behavior of animals using the Open Field test, immuno-hematological status, and morpho-functional changes in the central nervous system of mice. Irradiated animals displayed significantly different behavior in the OF in comparison with the control group. The radiation damage was confirmed by assessing the ratio of leukocytes in the peripheral blood of mice at a later date after exposure to Co60. After irradiation, a decrease in the glioneuronal complex was observed in the irritated group as well as histological changes of brain cells. To sum up, not only was the hematological status of mice altered upon the total gamma irradiation, but also their behavior, which was most probably due to significant alterations in the CNS. Graphical Abstract: Study of influence of ionizing radiation on female mice, comparison between different age groups. Open Field test on the 30 days after 2 Gy of γ-rays and histological analysis indicated changes in behavioral patterns, leucocytes, and brain tissue. [Figure not available: see fulltext.] © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Cellular and Molecular Neurobiology
T1  - The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes
VL  - InPress
DO  - 10.1007/s10571-023-01381-1
ER  - 

@article{
author = "Kolesnikova, I. A. and Lalkovičova, M. and Severyukhin, Yu. S. and Golikova, K. N. and Utina, D. M. and Pronskikh, E. V. and Despotović, Sanja Z. and Gaevsky, V. N. and Pirić, David and Masnikosa, Romana and Budennaya, N. N.",
year = "2023",
abstract = "We designed a study with the objective to determine the long-term radiation effects of gamma rays, originating from a single shot of Co60 at a dose of 2 Gy on the 7-month-old male mice of the ICR line in 30 days after the irradiation. The aim of this study was to characterize the behavior of animals using the Open Field test, immuno-hematological status, and morpho-functional changes in the central nervous system of mice. Irradiated animals displayed significantly different behavior in the OF in comparison with the control group. The radiation damage was confirmed by assessing the ratio of leukocytes in the peripheral blood of mice at a later date after exposure to Co60. After irradiation, a decrease in the glioneuronal complex was observed in the irritated group as well as histological changes of brain cells. To sum up, not only was the hematological status of mice altered upon the total gamma irradiation, but also their behavior, which was most probably due to significant alterations in the CNS. Graphical Abstract: Study of influence of ionizing radiation on female mice, comparison between different age groups. Open Field test on the 30 days after 2 Gy of γ-rays and histological analysis indicated changes in behavioral patterns, leucocytes, and brain tissue. [Figure not available: see fulltext.] © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Cellular and Molecular Neurobiology",
title = "The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes",
volume = "InPress",
doi = "10.1007/s10571-023-01381-1"
}

Kolesnikova, I. A., Lalkovičova, M., Severyukhin, Yu. S., Golikova, K. N., Utina, D. M., Pronskikh, E. V., Despotović, S. Z., Gaevsky, V. N., Pirić, D., Masnikosa, R.,& Budennaya, N. N.. (2023). The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes. in Cellular and Molecular Neurobiology, InPress.
https://doi.org/10.1007/s10571-023-01381-1

Kolesnikova IA, Lalkovičova M, Severyukhin YS, Golikova KN, Utina DM, Pronskikh EV, Despotović SZ, Gaevsky VN, Pirić D, Masnikosa R, Budennaya NN. The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes. in Cellular and Molecular Neurobiology. 2023;InPress.
doi:10.1007/s10571-023-01381-1 .

Kolesnikova, I. A., Lalkovičova, M., Severyukhin, Yu. S., Golikova, K. N., Utina, D. M., Pronskikh, E. V., Despotović, Sanja Z., Gaevsky, V. N., Pirić, David, Masnikosa, Romana, Budennaya, N. N., "The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes" in Cellular and Molecular Neurobiology, InPress (2023),
https://doi.org/10.1007/s10571-023-01381-1 . .

TY  - JOUR
AU  - Rilak Simović, Ana
AU  - Masnikosa, Romana
AU  - Bratsos, Ioannis
AU  - Alessio, Enzo
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8433
AB  - In this review we summarize our work on development of Ru complexes with potential antitumor activity, which was performed over the last few years. In order to establish the structure-activity relationship for Ru(II) compounds, we have designed, synthesized and thoroughly studied several Ru(II) complexes, which were divided in three main groups: i) organometallic Ru(II)-arene complexes, ii) Ru(II) half-sandwich coordination complexes bearing neutral face-capping macrocyclic ligands, such as 1,4,7-trithiacyclononane ([9]aneS3) and 1,4,7-triazacyclononane ([9]aneN3), and iii) Ru(II)-polypyridyl complexes. Our most recent experiments moved toward synthesis, chemistry and reactivity of the heteronuclear ruthenium(II)/ferrocene complexes. The first part of the present review gives a brief overview of the structural features and anticancer activity of ruthenium complexes. The second part is focused mainly on the results obtained from the kinetic and mechanistic studies of the reactions between Ru(II) complexes and guanine derivatives, such as 9-methylguanine (9MeG), guanosine (Guo) and guanosine-5′-monophosphate (5′-GMP), as well as on structural characterization of the final products of these reactions. In the final part we deal with the reactions of Ru(II) complexes with DNA, which is widely accepted as a potential target for cytotoxic ruthenium compounds. We have also described the interactions of Ru(II) compounds with the most abundant transport proteins from human serum: human serum albumin (HSA) and transferrin (Tf). We believe that a systematic review of the aforementioned studies will not only contribute to the future development of ruthenium complexes as potential antitumor agents, but will also help to understand the potential toxicity of ruthenium-based drugs. © 2019 Elsevier B.V.
T2  - Coordination Chemistry Reviews
T1  - Chemistry and reactivity of ruthenium(II) complexes: DNA/protein binding mode and anticancer activity are related to the complex structure
VL  - 398
SP  - 113011
DO  - 10.1016/j.ccr.2019.07.008
ER  - 

@article{
author = "Rilak Simović, Ana and Masnikosa, Romana and Bratsos, Ioannis and Alessio, Enzo",
year = "2019",
abstract = "In this review we summarize our work on development of Ru complexes with potential antitumor activity, which was performed over the last few years. In order to establish the structure-activity relationship for Ru(II) compounds, we have designed, synthesized and thoroughly studied several Ru(II) complexes, which were divided in three main groups: i) organometallic Ru(II)-arene complexes, ii) Ru(II) half-sandwich coordination complexes bearing neutral face-capping macrocyclic ligands, such as 1,4,7-trithiacyclononane ([9]aneS3) and 1,4,7-triazacyclononane ([9]aneN3), and iii) Ru(II)-polypyridyl complexes. Our most recent experiments moved toward synthesis, chemistry and reactivity of the heteronuclear ruthenium(II)/ferrocene complexes. The first part of the present review gives a brief overview of the structural features and anticancer activity of ruthenium complexes. The second part is focused mainly on the results obtained from the kinetic and mechanistic studies of the reactions between Ru(II) complexes and guanine derivatives, such as 9-methylguanine (9MeG), guanosine (Guo) and guanosine-5′-monophosphate (5′-GMP), as well as on structural characterization of the final products of these reactions. In the final part we deal with the reactions of Ru(II) complexes with DNA, which is widely accepted as a potential target for cytotoxic ruthenium compounds. We have also described the interactions of Ru(II) compounds with the most abundant transport proteins from human serum: human serum albumin (HSA) and transferrin (Tf). We believe that a systematic review of the aforementioned studies will not only contribute to the future development of ruthenium complexes as potential antitumor agents, but will also help to understand the potential toxicity of ruthenium-based drugs. © 2019 Elsevier B.V.",
journal = "Coordination Chemistry Reviews",
title = "Chemistry and reactivity of ruthenium(II) complexes: DNA/protein binding mode and anticancer activity are related to the complex structure",
volume = "398",
pages = "113011",
doi = "10.1016/j.ccr.2019.07.008"
}

Rilak Simović, A., Masnikosa, R., Bratsos, I.,& Alessio, E.. (2019). Chemistry and reactivity of ruthenium(II) complexes: DNA/protein binding mode and anticancer activity are related to the complex structure. in Coordination Chemistry Reviews, 398, 113011.
https://doi.org/10.1016/j.ccr.2019.07.008

Rilak Simović A, Masnikosa R, Bratsos I, Alessio E. Chemistry and reactivity of ruthenium(II) complexes: DNA/protein binding mode and anticancer activity are related to the complex structure. in Coordination Chemistry Reviews. 2019;398:113011.
doi:10.1016/j.ccr.2019.07.008 .

Rilak Simović, Ana, Masnikosa, Romana, Bratsos, Ioannis, Alessio, Enzo, "Chemistry and reactivity of ruthenium(II) complexes: DNA/protein binding mode and anticancer activity are related to the complex structure" in Coordination Chemistry Reviews, 398 (2019):113011,
https://doi.org/10.1016/j.ccr.2019.07.008 . .

TY  - JOUR
AU  - Nišavić, Marija
AU  - Stoiljković, Milovan
AU  - Crnolatac, Ivo
AU  - Milošević, Maja
AU  - Rilak, Ana
AU  - Masnikosa, Romana
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1878535216301198
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7665
AB  - Three coordination compounds of ruthenium(II), belonging to a recently synthesised series of water-soluble compounds of general formula mer-{[}Ru(L3)(N-N) Cl] Cl, where L3 = 4'-chloro2,2': 6', 200-terpyridine (Cl-tpy), N-N= ethylenediamine (en), 1,2-diaminocyclohexane (dach) or 2,2'bipyridine (bpy), have shown strong binding to calf thymus DNA and moderate in vitro cytotoxicity towards cancer cell lines. Knowing that serum proteins play a crucial role in the transport and deactivation of ruthenium drugs, we have conducted a detailed study of their interactions with two major metal-transporting serum proteins, albumin and transferrin, and it is presented herein. Ruthenated protein adducts were formed with various concentrations of the three compounds and then separated from the unbound portions by ultrafiltration through 10 kDa cut-off centrifugal filter units. The stoichiometry of binding was determined using inductively coupled plasma optical emission spectrometry. One mol of albumin bound up to 7, 8.5 and 1.5 mol of compound 1 ({[}Ru(Cltpy)(en) Cl]{[}Cl]), 2 ({[}Ru(Cl-tpy)(dach) Cl]{[}Cl] and 3 ({[}Ru(Cl-tpy)(bpy) Cl]{[}Cl]), respectively. One mol of transferrin bound up to 3, 3.5 and 0.4 mol of 1, 2 and 3, respectively. The affinity of albumin and transferrin for the three ruthenium compounds was evaluated using fluorescence quenching. The binding constants for 1 and 2 lay within the range 10(4) -10(5) M - 1, suggesting moderate-to-strong attachment to albumin. Both compounds showed much lower affinity for transferrin (10(2) -10(3) M - 1). Compound 3 bound weakly to each studied protein. High resolution ESI qTOF mass spectra of albumin before and after binding of 1 revealed the high stoichiometry of binding. Although the binding of the compounds 1-3 to albumin and transferrin did not affect proteins' secondary structure much, their tertiary structures underwent some alterations, as deduced from the circular dichroism study. Changes in the stability of albumin, after binding to compounds 1-3 were examined by differential scanning calorimetry. (C) 2016 The Authors. Production and hosting by Elsevier B. V. on behalf of King Saud University. This is an open access article under theCCBY-NC-NDlicense.
T2  - Arabian Journal of Chemistry
T1  - Highly water-soluble ruthenium(II) terpyridine coordination compounds form stable adducts with blood-borne metal transporting proteins
VL  - 11
IS  - 3
SP  - 291
EP  - 304
DO  - 10.1016/j.arabjc.2016.07.021
ER  - 

@article{
author = "Nišavić, Marija and Stoiljković, Milovan and Crnolatac, Ivo and Milošević, Maja and Rilak, Ana and Masnikosa, Romana",
year = "2018",
abstract = "Three coordination compounds of ruthenium(II), belonging to a recently synthesised series of water-soluble compounds of general formula mer-{[}Ru(L3)(N-N) Cl] Cl, where L3 = 4'-chloro2,2': 6', 200-terpyridine (Cl-tpy), N-N= ethylenediamine (en), 1,2-diaminocyclohexane (dach) or 2,2'bipyridine (bpy), have shown strong binding to calf thymus DNA and moderate in vitro cytotoxicity towards cancer cell lines. Knowing that serum proteins play a crucial role in the transport and deactivation of ruthenium drugs, we have conducted a detailed study of their interactions with two major metal-transporting serum proteins, albumin and transferrin, and it is presented herein. Ruthenated protein adducts were formed with various concentrations of the three compounds and then separated from the unbound portions by ultrafiltration through 10 kDa cut-off centrifugal filter units. The stoichiometry of binding was determined using inductively coupled plasma optical emission spectrometry. One mol of albumin bound up to 7, 8.5 and 1.5 mol of compound 1 ({[}Ru(Cltpy)(en) Cl]{[}Cl]), 2 ({[}Ru(Cl-tpy)(dach) Cl]{[}Cl] and 3 ({[}Ru(Cl-tpy)(bpy) Cl]{[}Cl]), respectively. One mol of transferrin bound up to 3, 3.5 and 0.4 mol of 1, 2 and 3, respectively. The affinity of albumin and transferrin for the three ruthenium compounds was evaluated using fluorescence quenching. The binding constants for 1 and 2 lay within the range 10(4) -10(5) M - 1, suggesting moderate-to-strong attachment to albumin. Both compounds showed much lower affinity for transferrin (10(2) -10(3) M - 1). Compound 3 bound weakly to each studied protein. High resolution ESI qTOF mass spectra of albumin before and after binding of 1 revealed the high stoichiometry of binding. Although the binding of the compounds 1-3 to albumin and transferrin did not affect proteins' secondary structure much, their tertiary structures underwent some alterations, as deduced from the circular dichroism study. Changes in the stability of albumin, after binding to compounds 1-3 were examined by differential scanning calorimetry. (C) 2016 The Authors. Production and hosting by Elsevier B. V. on behalf of King Saud University. This is an open access article under theCCBY-NC-NDlicense.",
journal = "Arabian Journal of Chemistry",
title = "Highly water-soluble ruthenium(II) terpyridine coordination compounds form stable adducts with blood-borne metal transporting proteins",
volume = "11",
number = "3",
pages = "291-304",
doi = "10.1016/j.arabjc.2016.07.021"
}

Nišavić, M., Stoiljković, M., Crnolatac, I., Milošević, M., Rilak, A.,& Masnikosa, R.. (2018). Highly water-soluble ruthenium(II) terpyridine coordination compounds form stable adducts with blood-borne metal transporting proteins. in Arabian Journal of Chemistry, 11(3), 291-304.
https://doi.org/10.1016/j.arabjc.2016.07.021

Nišavić M, Stoiljković M, Crnolatac I, Milošević M, Rilak A, Masnikosa R. Highly water-soluble ruthenium(II) terpyridine coordination compounds form stable adducts with blood-borne metal transporting proteins. in Arabian Journal of Chemistry. 2018;11(3):291-304.
doi:10.1016/j.arabjc.2016.07.021 .

Nišavić, Marija, Stoiljković, Milovan, Crnolatac, Ivo, Milošević, Maja, Rilak, Ana, Masnikosa, Romana, "Highly water-soluble ruthenium(II) terpyridine coordination compounds form stable adducts with blood-borne metal transporting proteins" in Arabian Journal of Chemistry, 11, no. 3 (2018):291-304,
https://doi.org/10.1016/j.arabjc.2016.07.021 . .

TY  - JOUR
AU  - Milutinović, Milan M.
AU  - Čanović, Petar P.
AU  - Stevanović, Dragana D.
AU  - Masnikosa, Romana
AU  - Vraneš, Milan
AU  - Tot, Aleksandar
AU  - Zarić, Milan M.
AU  - Marković-Simović, Bojana
AU  - Misirkić-Marjanović, Maja
AU  - Vučićević, Ljubica
AU  - Savić, Maja
AU  - Jakovljević, Vladimir Lj.
AU  - Trajković, Vladimir S.
AU  - Volarević, Vladislav
AU  - Kanjevac, Tatjana
AU  - Rilak Simović, Ana
PY  - 2018
UR  - http://pubs.acs.org/doi/10.1021/acs.organomet.8b00604
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7967
AB  - The two new heterometallic Ru(II)-tpy/ferrocene complexes [Ru(tpy)Cl2(mtefc)] (1) and [Ru(tpy)Cl2(mtpfc)] (2) (where tpy = 2,2′:6′,2′′-terpyridine, mtefc = (2-(methylthio)ethyl)ferrocene, and mtpfc = (3-(methylthio)propyl)ferrocene) have been synthesized and then characterized through elemental analysis, followed by various spectroscopic (IR, UV-vis, 1D and 2D NMR) and mass spectrometric techniques (MALDI TOF and ESI Q-TOF MS). UV-vis and fluorescence spectroscopy and viscometry were employed to study the interactions of the complexes 1 and 2 with calf thymus DNA. Both 1 and 2 expelled ethidium bromide (EB) from the EB/DNA complex (Ksv = (1.5-1.8) × 104 M-1), which suggested that the complexes intercalated into the double helix of DNA. Both complexes strongly quenched the fluorescence of tryptophan residues in serum albumin through both static and dynamic quenching. Molecular docking confirmed the intercalative mode of complex interaction with DNA. The docking results implied that 1 and 2 interacted with hydrophobic residues of albumin, particularly with those lying in the proximity of Tyr 160. We here demonstrate the high cytotoxic potential of complexes 1 and 2 against the breast cancer cells that originated either from humans (MDA-MB-231) or from mice (4T1), with apoptosis being the main mechanism of complex-induced cell death. It is worth noting that both complexes promoted activation of innate and acquired antitumor immunity, which contributed to the reduced growth and progression of mammary carcinoma in vivo. Copyright © 2018 American Chemical Society.
T2  - Organometallics
T1  - Newly Synthesized Heteronuclear Ruthenium(II)/Ferrocene Complexes Suppress the Growth of Mammary Carcinoma in 4T1-Treated BALB/c Mice by Promoting Activation of Antitumor Immunity
VL  - 37
IS  - 22
SP  - 4250
EP  - 4266
DO  - 10.1021/acs.organomet.8b00604
ER  - 

@article{
author = "Milutinović, Milan M. and Čanović, Petar P. and Stevanović, Dragana D. and Masnikosa, Romana and Vraneš, Milan and Tot, Aleksandar and Zarić, Milan M. and Marković-Simović, Bojana and Misirkić-Marjanović, Maja and Vučićević, Ljubica and Savić, Maja and Jakovljević, Vladimir Lj. and Trajković, Vladimir S. and Volarević, Vladislav and Kanjevac, Tatjana and Rilak Simović, Ana",
year = "2018",
abstract = "The two new heterometallic Ru(II)-tpy/ferrocene complexes [Ru(tpy)Cl2(mtefc)] (1) and [Ru(tpy)Cl2(mtpfc)] (2) (where tpy = 2,2′:6′,2′′-terpyridine, mtefc = (2-(methylthio)ethyl)ferrocene, and mtpfc = (3-(methylthio)propyl)ferrocene) have been synthesized and then characterized through elemental analysis, followed by various spectroscopic (IR, UV-vis, 1D and 2D NMR) and mass spectrometric techniques (MALDI TOF and ESI Q-TOF MS). UV-vis and fluorescence spectroscopy and viscometry were employed to study the interactions of the complexes 1 and 2 with calf thymus DNA. Both 1 and 2 expelled ethidium bromide (EB) from the EB/DNA complex (Ksv = (1.5-1.8) × 104 M-1), which suggested that the complexes intercalated into the double helix of DNA. Both complexes strongly quenched the fluorescence of tryptophan residues in serum albumin through both static and dynamic quenching. Molecular docking confirmed the intercalative mode of complex interaction with DNA. The docking results implied that 1 and 2 interacted with hydrophobic residues of albumin, particularly with those lying in the proximity of Tyr 160. We here demonstrate the high cytotoxic potential of complexes 1 and 2 against the breast cancer cells that originated either from humans (MDA-MB-231) or from mice (4T1), with apoptosis being the main mechanism of complex-induced cell death. It is worth noting that both complexes promoted activation of innate and acquired antitumor immunity, which contributed to the reduced growth and progression of mammary carcinoma in vivo. Copyright © 2018 American Chemical Society.",
journal = "Organometallics",
title = "Newly Synthesized Heteronuclear Ruthenium(II)/Ferrocene Complexes Suppress the Growth of Mammary Carcinoma in 4T1-Treated BALB/c Mice by Promoting Activation of Antitumor Immunity",
volume = "37",
number = "22",
pages = "4250-4266",
doi = "10.1021/acs.organomet.8b00604"
}

Milutinović, M. M., Čanović, P. P., Stevanović, D. D., Masnikosa, R., Vraneš, M., Tot, A., Zarić, M. M., Marković-Simović, B., Misirkić-Marjanović, M., Vučićević, L., Savić, M., Jakovljević, V. Lj., Trajković, V. S., Volarević, V., Kanjevac, T.,& Rilak Simović, A.. (2018). Newly Synthesized Heteronuclear Ruthenium(II)/Ferrocene Complexes Suppress the Growth of Mammary Carcinoma in 4T1-Treated BALB/c Mice by Promoting Activation of Antitumor Immunity. in Organometallics, 37(22), 4250-4266.
https://doi.org/10.1021/acs.organomet.8b00604

Milutinović MM, Čanović PP, Stevanović DD, Masnikosa R, Vraneš M, Tot A, Zarić MM, Marković-Simović B, Misirkić-Marjanović M, Vučićević L, Savić M, Jakovljević VL, Trajković VS, Volarević V, Kanjevac T, Rilak Simović A. Newly Synthesized Heteronuclear Ruthenium(II)/Ferrocene Complexes Suppress the Growth of Mammary Carcinoma in 4T1-Treated BALB/c Mice by Promoting Activation of Antitumor Immunity. in Organometallics. 2018;37(22):4250-4266.
doi:10.1021/acs.organomet.8b00604 .

Milutinović, Milan M., Čanović, Petar P., Stevanović, Dragana D., Masnikosa, Romana, Vraneš, Milan, Tot, Aleksandar, Zarić, Milan M., Marković-Simović, Bojana, Misirkić-Marjanović, Maja, Vučićević, Ljubica, Savić, Maja, Jakovljević, Vladimir Lj., Trajković, Vladimir S., Volarević, Vladislav, Kanjevac, Tatjana, Rilak Simović, Ana, "Newly Synthesized Heteronuclear Ruthenium(II)/Ferrocene Complexes Suppress the Growth of Mammary Carcinoma in 4T1-Treated BALB/c Mice by Promoting Activation of Antitumor Immunity" in Organometallics, 37, no. 22 (2018):4250-4266,
https://doi.org/10.1021/acs.organomet.8b00604 . .

TY  - JOUR
AU  - Radisavljević, Snežana
AU  - Bratsos, Ioannis
AU  - Scheurer, Andreas
AU  - Korzekwa, Jana
AU  - Masnikosa, Romana
AU  - Tot, Aleksandar
AU  - Gligorijević, Nevenka N.
AU  - Radulović, Siniša S.
AU  - Rilak Simović, Ana
PY  - 2018
UR  - http://xlink.rsc.org/?DOI=C8DT02903B
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7894
AB  - With the aim of assessing whether Au(iii) compounds with pincer type ligands might be utilized as potential antitumor agents, three new monofunctional Au(iii) complexes of the general formula [Au(N-N'-N)Cl]Cl-2, where N-N'-N = 2,6-bis(5-tert-butyl-1H-pyrazol-3-yl)pyridine (H2LtBu, 1), 2,6-bis(5-tert-butyl-1-methyl-1H-pyrazol-3-yl)pyridine (Me2LtBu, 2) or 2,6-bis((4S,7R)-1,7,8,8-tetramethyl-4,5,6,7-tetrahydro-1H-4,7-methanoindazol-3-yl)pyridine (Me-2*L, 3) were synthesized. All complexes were characterized by elemental analysis, spectroscopic techniques (IR, UV-Vis, 1D and 2D NMR) and mass spectrometry (MALDI TOF MS). The chemical behavior of the complexes under physiological conditions was studied by UV-Vis spectroscopy, which showed that all compounds were remarkably stable and that the gold center remained in the 3+ oxidation state. The kinetics and the mechanism of the reaction of complexes 1-3 with guanine derivatives (i.e. guanosine (Guo) and guanosine-5-monophosphate (5-GMP)) and calf thymus DNA (CT DNA) were studied by stopped-flow spectroscopy. The three complexes displayed moderately different rate constants in their reactions with Guo, 5-GMP and CT DNA, which can be explained by the steric hindrance and sigma-donicity of the methyl substituent on the bis-pyrazolylpyridine fragment in complexes 2 and 3. The measured enthalpies and entropies of activation (Delta H-not equal > 0, Delta S-not equal < 0) supported an associative mechanism for the substitution process. The interaction of the newly synthesized complexes 1-3 with CT DNA was investigated by UV-Vis and fluorescence spectroscopy, and also by viscosity measurements, which all indicated that complexes 1-3 bound to CT DNA with moderate binding affinity (K-b = 1.6-5.7 x 10(3) M-1) and stabilized the duplex of CT DNA. Molecular docking indicated that complexes 1-3 interacted with DNA via intercalation. Complex 1 reduced the cell survival of all the investigated cell lines (A549, A375, and LS-174) with IC50 values being up to 20 mu M. We have shown that 1 induced perturbations of the cell cycle and led to apoptosis in human melanoma A375 cells. Complex 1 also affected the level of reactive oxygen species (ROS) in the same cells. However, pre-treatment of A375 cells with NAC (ROS scavenger) reversed the effect of 1 on their survival.
T2  - Dalton Transactions
T1  - New gold pincer-type complexes: synthesis, characterization, DNA binding studies and cytotoxicity
VL  - 47
IS  - 38
SP  - 13696
EP  - 13712
DO  - 10.1039/C8DT02903B
ER  - 

@article{
author = "Radisavljević, Snežana and Bratsos, Ioannis and Scheurer, Andreas and Korzekwa, Jana and Masnikosa, Romana and Tot, Aleksandar and Gligorijević, Nevenka N. and Radulović, Siniša S. and Rilak Simović, Ana",
year = "2018",
abstract = "With the aim of assessing whether Au(iii) compounds with pincer type ligands might be utilized as potential antitumor agents, three new monofunctional Au(iii) complexes of the general formula [Au(N-N'-N)Cl]Cl-2, where N-N'-N = 2,6-bis(5-tert-butyl-1H-pyrazol-3-yl)pyridine (H2LtBu, 1), 2,6-bis(5-tert-butyl-1-methyl-1H-pyrazol-3-yl)pyridine (Me2LtBu, 2) or 2,6-bis((4S,7R)-1,7,8,8-tetramethyl-4,5,6,7-tetrahydro-1H-4,7-methanoindazol-3-yl)pyridine (Me-2*L, 3) were synthesized. All complexes were characterized by elemental analysis, spectroscopic techniques (IR, UV-Vis, 1D and 2D NMR) and mass spectrometry (MALDI TOF MS). The chemical behavior of the complexes under physiological conditions was studied by UV-Vis spectroscopy, which showed that all compounds were remarkably stable and that the gold center remained in the 3+ oxidation state. The kinetics and the mechanism of the reaction of complexes 1-3 with guanine derivatives (i.e. guanosine (Guo) and guanosine-5-monophosphate (5-GMP)) and calf thymus DNA (CT DNA) were studied by stopped-flow spectroscopy. The three complexes displayed moderately different rate constants in their reactions with Guo, 5-GMP and CT DNA, which can be explained by the steric hindrance and sigma-donicity of the methyl substituent on the bis-pyrazolylpyridine fragment in complexes 2 and 3. The measured enthalpies and entropies of activation (Delta H-not equal > 0, Delta S-not equal < 0) supported an associative mechanism for the substitution process. The interaction of the newly synthesized complexes 1-3 with CT DNA was investigated by UV-Vis and fluorescence spectroscopy, and also by viscosity measurements, which all indicated that complexes 1-3 bound to CT DNA with moderate binding affinity (K-b = 1.6-5.7 x 10(3) M-1) and stabilized the duplex of CT DNA. Molecular docking indicated that complexes 1-3 interacted with DNA via intercalation. Complex 1 reduced the cell survival of all the investigated cell lines (A549, A375, and LS-174) with IC50 values being up to 20 mu M. We have shown that 1 induced perturbations of the cell cycle and led to apoptosis in human melanoma A375 cells. Complex 1 also affected the level of reactive oxygen species (ROS) in the same cells. However, pre-treatment of A375 cells with NAC (ROS scavenger) reversed the effect of 1 on their survival.",
journal = "Dalton Transactions",
title = "New gold pincer-type complexes: synthesis, characterization, DNA binding studies and cytotoxicity",
volume = "47",
number = "38",
pages = "13696-13712",
doi = "10.1039/C8DT02903B"
}

Radisavljević, S., Bratsos, I., Scheurer, A., Korzekwa, J., Masnikosa, R., Tot, A., Gligorijević, N. N., Radulović, S. S.,& Rilak Simović, A.. (2018). New gold pincer-type complexes: synthesis, characterization, DNA binding studies and cytotoxicity. in Dalton Transactions, 47(38), 13696-13712.
https://doi.org/10.1039/C8DT02903B

Radisavljević S, Bratsos I, Scheurer A, Korzekwa J, Masnikosa R, Tot A, Gligorijević NN, Radulović SS, Rilak Simović A. New gold pincer-type complexes: synthesis, characterization, DNA binding studies and cytotoxicity. in Dalton Transactions. 2018;47(38):13696-13712.
doi:10.1039/C8DT02903B .

Radisavljević, Snežana, Bratsos, Ioannis, Scheurer, Andreas, Korzekwa, Jana, Masnikosa, Romana, Tot, Aleksandar, Gligorijević, Nevenka N., Radulović, Siniša S., Rilak Simović, Ana, "New gold pincer-type complexes: synthesis, characterization, DNA binding studies and cytotoxicity" in Dalton Transactions, 47, no. 38 (2018):13696-13712,
https://doi.org/10.1039/C8DT02903B . .

TY  - JOUR
AU  - Cvetković, Zorica
AU  - Milošević, Maja
AU  - Cvetković, Bora
AU  - Masnikosa, Romana
AU  - Arsić, Aleksandra
AU  - Petrović, Snježana
AU  - Vučić, Vesna
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1489
AB  - Limited studies have been performed to associate abnormal phospholipid (PL) profile and disease activity in hematological malignancies, including non-Hodgkin lymphoma (NHL). The aim of his study was to evaluate the levels of plasma PL fractions in NHL patients, in response to chemotherapy. Forty non-treated patients with NHL and 25 healthy individuals were recruited. Blood samples from patients were taken before chemotherapy, after 3 cycles and after the end of the treatment, and PL fractions were resolved by one-dimensional thin-layer chromatography. To assess potential relationship between plasma PL profile and response to therapy, patients were divided according to clinical outcome in 3 groups: complete remission (CR), stable disease (SD) and progression (PG). Despite significant differences between NHL patients and healthy controls, no differences were found at baseline among patients divided according to clinical outcome. During and after chemotherapy important alterations in PL profile were observed. Levels of total PLs and all PL fractions decreased in patients with PG while in patients who responded to therapy (CR, SD) PLs significantly increased. Results of our study suggest that changes of total PLs and PL fractions during the therapy are associated with the effects of therapy and clinical outcome in patients with NHL. (C) 2017 Elsevier Ltd. All rights reserved.
T2  - Leukemia Research
T1  - Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients
VL  - 54
SP  - 39
EP  - 46
DO  - 10.1016/j.leukres.2017.01.004
ER  - 

@article{
author = "Cvetković, Zorica and Milošević, Maja and Cvetković, Bora and Masnikosa, Romana and Arsić, Aleksandra and Petrović, Snježana and Vučić, Vesna",
year = "2017",
abstract = "Limited studies have been performed to associate abnormal phospholipid (PL) profile and disease activity in hematological malignancies, including non-Hodgkin lymphoma (NHL). The aim of his study was to evaluate the levels of plasma PL fractions in NHL patients, in response to chemotherapy. Forty non-treated patients with NHL and 25 healthy individuals were recruited. Blood samples from patients were taken before chemotherapy, after 3 cycles and after the end of the treatment, and PL fractions were resolved by one-dimensional thin-layer chromatography. To assess potential relationship between plasma PL profile and response to therapy, patients were divided according to clinical outcome in 3 groups: complete remission (CR), stable disease (SD) and progression (PG). Despite significant differences between NHL patients and healthy controls, no differences were found at baseline among patients divided according to clinical outcome. During and after chemotherapy important alterations in PL profile were observed. Levels of total PLs and all PL fractions decreased in patients with PG while in patients who responded to therapy (CR, SD) PLs significantly increased. Results of our study suggest that changes of total PLs and PL fractions during the therapy are associated with the effects of therapy and clinical outcome in patients with NHL. (C) 2017 Elsevier Ltd. All rights reserved.",
journal = "Leukemia Research",
title = "Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients",
volume = "54",
pages = "39-46",
doi = "10.1016/j.leukres.2017.01.004"
}

Cvetković, Z., Milošević, M., Cvetković, B., Masnikosa, R., Arsić, A., Petrović, S.,& Vučić, V.. (2017). Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients. in Leukemia Research, 54, 39-46.
https://doi.org/10.1016/j.leukres.2017.01.004

Cvetković Z, Milošević M, Cvetković B, Masnikosa R, Arsić A, Petrović S, Vučić V. Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients. in Leukemia Research. 2017;54:39-46.
doi:10.1016/j.leukres.2017.01.004 .

Cvetković, Zorica, Milošević, Maja, Cvetković, Bora, Masnikosa, Romana, Arsić, Aleksandra, Petrović, Snježana, Vučić, Vesna, "Plasma phospholipid changes are associated with response to chemotherapy in non-Hodgkin lymphoma patients" in Leukemia Research, 54 (2017):39-46,
https://doi.org/10.1016/j.leukres.2017.01.004 . .

TY  - JOUR
AU  - Robajac, Dragana
AU  - Vanhooren, Valerie
AU  - Masnikosa, Romana
AU  - Miković, Zeljko
AU  - Mandić, Vesna
AU  - Libert, Claude
AU  - Nedić, Olgica
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/934
AB  - Posttranslational modifications (PTM) which accompany pathological conditions affect protein structure, characteristics and modulate its activity. Glycosylation is one of the most frequent PTM influencing protein folding, localisation and function. Hypertension is a common gestational complication, which can lead to foetal growth restriction (IUGR) and even to foetal or maternal death. In this work we focused on the impact of preeclampsia complicated with IUGR on placental membrane N-glycome. Results have shown that preeclampsia reduced fucosylation of placental glycans, increased the appearance of paucimannosidic and mannosidic structures with lower number of mannose residues and decreased the amount of glycans with more mannose residues. Since preeclampsia is tightly connected to IUGR, glycosylation changes were investigated also on the functional membrane receptors responsible for growth: insulin receptor and the type 1 insulin-like growth factor receptor (IR and IGF1R). It was found that IR present in the IUGR placenta contained significantly less alpha 2,6-Sia. Therefore, glycans on placental membranes alter due to preeclampsia, but changes seen at the level of the entire N-glycome may be different from the changes detected at the level of a specific glycoprotein. The difference recorded due to pathology in one membrane molecule (IR) was not found in another homologous molecule (IGF1R). Thus, besides studying the glycosylation pattern of the entire placental membrane due to preeclampsia, it is inevitable to study directly glycoprotein of interest, as no general assumptions or extrapolations can be made. (C) 2015 Elsevier Inc All rights reserved.
PB  - Elsevier
T2  - Experimental and Molecular Pathology
T1  - Preeclampsia transforms membrane N-glycome in human placenta
VL  - 100
IS  - 1
SP  - 26
EP  - 30
DO  - 10.1016/j.yexmp.2015.11.029
ER  - 

@article{
author = "Robajac, Dragana and Vanhooren, Valerie and Masnikosa, Romana and Miković, Zeljko and Mandić, Vesna and Libert, Claude and Nedić, Olgica",
year = "2016",
abstract = "Posttranslational modifications (PTM) which accompany pathological conditions affect protein structure, characteristics and modulate its activity. Glycosylation is one of the most frequent PTM influencing protein folding, localisation and function. Hypertension is a common gestational complication, which can lead to foetal growth restriction (IUGR) and even to foetal or maternal death. In this work we focused on the impact of preeclampsia complicated with IUGR on placental membrane N-glycome. Results have shown that preeclampsia reduced fucosylation of placental glycans, increased the appearance of paucimannosidic and mannosidic structures with lower number of mannose residues and decreased the amount of glycans with more mannose residues. Since preeclampsia is tightly connected to IUGR, glycosylation changes were investigated also on the functional membrane receptors responsible for growth: insulin receptor and the type 1 insulin-like growth factor receptor (IR and IGF1R). It was found that IR present in the IUGR placenta contained significantly less alpha 2,6-Sia. Therefore, glycans on placental membranes alter due to preeclampsia, but changes seen at the level of the entire N-glycome may be different from the changes detected at the level of a specific glycoprotein. The difference recorded due to pathology in one membrane molecule (IR) was not found in another homologous molecule (IGF1R). Thus, besides studying the glycosylation pattern of the entire placental membrane due to preeclampsia, it is inevitable to study directly glycoprotein of interest, as no general assumptions or extrapolations can be made. (C) 2015 Elsevier Inc All rights reserved.",
publisher = "Elsevier",
journal = "Experimental and Molecular Pathology",
title = "Preeclampsia transforms membrane N-glycome in human placenta",
volume = "100",
number = "1",
pages = "26-30",
doi = "10.1016/j.yexmp.2015.11.029"
}

Robajac, D., Vanhooren, V., Masnikosa, R., Miković, Z., Mandić, V., Libert, C.,& Nedić, O.. (2016). Preeclampsia transforms membrane N-glycome in human placenta. in Experimental and Molecular Pathology
Elsevier., 100(1), 26-30.
https://doi.org/10.1016/j.yexmp.2015.11.029

Robajac D, Vanhooren V, Masnikosa R, Miković Z, Mandić V, Libert C, Nedić O. Preeclampsia transforms membrane N-glycome in human placenta. in Experimental and Molecular Pathology. 2016;100(1):26-30.
doi:10.1016/j.yexmp.2015.11.029 .

Robajac, Dragana, Vanhooren, Valerie, Masnikosa, Romana, Miković, Zeljko, Mandić, Vesna, Libert, Claude, Nedić, Olgica, "Preeclampsia transforms membrane N-glycome in human placenta" in Experimental and Molecular Pathology, 100, no. 1 (2016):26-30,
https://doi.org/10.1016/j.yexmp.2015.11.029 . .

TY  - JOUR
AU  - Robajac, Dragana
AU  - Masnikosa, Romana
AU  - Miković, Zeljko
AU  - Nedić, Olgica
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/994
AB  - Introduction: Insulin receptor (IR) and type 1 and type 2 insulin-like growth factor receptors (IGF1 R and IGF2R) play important roles in regulation of placental and foetal growth. All three receptors are abundantly glycosylated. N-glycosylation significantly affects protein conformation and may alter its function. We have recently found that the N-glycome of placental membrane proteins alters during gestation. The aim of the study presented herein was to investigate whether there were gestation-related changes in N-glycan profiles of placental IR and IGFRs. Methods: Placentas from healthy women at first (FTP) and third trimester (TTP) of pregnancy were collected, membrane proteins isolated, solubilised and used as the source of IR and IGFRs. Reactivity of glycoforms of IR and IGFRs with lectins was monitored by measuring radioactivity of I-125-ligands-receptors complexes. Results: Significant differences in the binding pattern of all three receptors to the lectins were observed between FTP and TTP, which suggest gestational changes in N-glycans bound to receptors. These changes include decrease in total fucosylated, core-fucosylated biantennary N-glycan (NA2F) and alpha 2,6-sialo-N-glycans (for IR); decrease in total fucosylated and a2,6-sialo-N-glycans and an increase in NA2F N-glycans (for IGF1R) and an increase in the total fucosylation and NA2F N-glycans (for IGF2R). Discussion: The gestational alterations in N-glycans attached to IR and IGFRs may represent a mechanism by which these receptors acquire new/additional roles as gestation progresses. (C) 2016 Elsevier Ltd. All rights reserved.
PB  - Elsevier
T2  - Placenta
T1  - Gestation-associated changes in the glycosylation of placental insulin and insulin-like growth factor receptors
VL  - 39
SP  - 70
EP  - 76
DO  - 10.1016/j.placenta.2016.01.005
ER  - 

@article{
author = "Robajac, Dragana and Masnikosa, Romana and Miković, Zeljko and Nedić, Olgica",
year = "2016",
abstract = "Introduction: Insulin receptor (IR) and type 1 and type 2 insulin-like growth factor receptors (IGF1 R and IGF2R) play important roles in regulation of placental and foetal growth. All three receptors are abundantly glycosylated. N-glycosylation significantly affects protein conformation and may alter its function. We have recently found that the N-glycome of placental membrane proteins alters during gestation. The aim of the study presented herein was to investigate whether there were gestation-related changes in N-glycan profiles of placental IR and IGFRs. Methods: Placentas from healthy women at first (FTP) and third trimester (TTP) of pregnancy were collected, membrane proteins isolated, solubilised and used as the source of IR and IGFRs. Reactivity of glycoforms of IR and IGFRs with lectins was monitored by measuring radioactivity of I-125-ligands-receptors complexes. Results: Significant differences in the binding pattern of all three receptors to the lectins were observed between FTP and TTP, which suggest gestational changes in N-glycans bound to receptors. These changes include decrease in total fucosylated, core-fucosylated biantennary N-glycan (NA2F) and alpha 2,6-sialo-N-glycans (for IR); decrease in total fucosylated and a2,6-sialo-N-glycans and an increase in NA2F N-glycans (for IGF1R) and an increase in the total fucosylation and NA2F N-glycans (for IGF2R). Discussion: The gestational alterations in N-glycans attached to IR and IGFRs may represent a mechanism by which these receptors acquire new/additional roles as gestation progresses. (C) 2016 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Placenta",
title = "Gestation-associated changes in the glycosylation of placental insulin and insulin-like growth factor receptors",
volume = "39",
pages = "70-76",
doi = "10.1016/j.placenta.2016.01.005"
}

Robajac, D., Masnikosa, R., Miković, Z.,& Nedić, O.. (2016). Gestation-associated changes in the glycosylation of placental insulin and insulin-like growth factor receptors. in Placenta
Elsevier., 39, 70-76.
https://doi.org/10.1016/j.placenta.2016.01.005

Robajac D, Masnikosa R, Miković Z, Nedić O. Gestation-associated changes in the glycosylation of placental insulin and insulin-like growth factor receptors. in Placenta. 2016;39:70-76.
doi:10.1016/j.placenta.2016.01.005 .

Robajac, Dragana, Masnikosa, Romana, Miković, Zeljko, Nedić, Olgica, "Gestation-associated changes in the glycosylation of placental insulin and insulin-like growth factor receptors" in Placenta, 39 (2016):70-76,
https://doi.org/10.1016/j.placenta.2016.01.005 . .

TY  - JOUR
AU  - Nišavić, Marija
AU  - Masnikosa, Romana
AU  - Butorac, Ana
AU  - Perica, Kristina
AU  - Rilak, Ana
AU  - Korićanac, Lela
AU  - Hozić, Amela
AU  - Petković, Marijana
AU  - Cindrić, Mario
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1124
AB  - Hyphenated mass spectrometry (MS) techniques have attained an important position in analysis of covalent and non-covalent interactions of metal complexes with peptides and proteins. The aim of the present study was to qualitatively and quantitatively determine ruthenium binding sites on a protein using tandem mass spectrometry and allied techniques, i.e. liquid chromatography (LC) and inductively coupled plasma optical emission spectrometry (ICP-OES). For that purpose, two newly synthesized Ru(II) complexes of a meridional geometry, namely mer-[Ru(4 Cl-tpy)(en)Cl](+) (1) and mer-[Ru(4 Cl-tpy)(dach)Cl](+) (2) (where 4 Cl-tpy = 4-chloro-2,2:6,2 -terpyridine, en = 1,2-diaminoethane and dach = 1,2-diaminocyclohexane), and bovine serum albumin were used. The binding of the complexes to the protein was investigated by means of size exclusion- and reversed phase-LC, ICP OES, matrix-assisted laser desorption ionization MS and MS/MS. Ruthenated peptide sequence and a binding target amino acid were revealed through accurate elucidation of MS/MS spectra. The results obtained in this study suggest a high binding capacity of the protein towards both complexes, with up to 5.77 +/- 0.14 and 6.95 +/- 0.43 mol of 1 and 2 bound per mol of protein, respectively. The proposed binding mechanism for the selected complexes includes the release of Cl ligand, its replacement with water molecule and further coordination to electron donor histidine residue. (C) 2016 Elsevier Inc. All rights reserved.
T2  - Journal of Inorganic Biochemistry
T1  - Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin
VL  - 159
SP  - 89
EP  - 95
DO  - 10.1016/j.jinorgbio.2016.02.034
ER  - 

@article{
author = "Nišavić, Marija and Masnikosa, Romana and Butorac, Ana and Perica, Kristina and Rilak, Ana and Korićanac, Lela and Hozić, Amela and Petković, Marijana and Cindrić, Mario",
year = "2016",
abstract = "Hyphenated mass spectrometry (MS) techniques have attained an important position in analysis of covalent and non-covalent interactions of metal complexes with peptides and proteins. The aim of the present study was to qualitatively and quantitatively determine ruthenium binding sites on a protein using tandem mass spectrometry and allied techniques, i.e. liquid chromatography (LC) and inductively coupled plasma optical emission spectrometry (ICP-OES). For that purpose, two newly synthesized Ru(II) complexes of a meridional geometry, namely mer-[Ru(4 Cl-tpy)(en)Cl](+) (1) and mer-[Ru(4 Cl-tpy)(dach)Cl](+) (2) (where 4 Cl-tpy = 4-chloro-2,2:6,2 -terpyridine, en = 1,2-diaminoethane and dach = 1,2-diaminocyclohexane), and bovine serum albumin were used. The binding of the complexes to the protein was investigated by means of size exclusion- and reversed phase-LC, ICP OES, matrix-assisted laser desorption ionization MS and MS/MS. Ruthenated peptide sequence and a binding target amino acid were revealed through accurate elucidation of MS/MS spectra. The results obtained in this study suggest a high binding capacity of the protein towards both complexes, with up to 5.77 +/- 0.14 and 6.95 +/- 0.43 mol of 1 and 2 bound per mol of protein, respectively. The proposed binding mechanism for the selected complexes includes the release of Cl ligand, its replacement with water molecule and further coordination to electron donor histidine residue. (C) 2016 Elsevier Inc. All rights reserved.",
journal = "Journal of Inorganic Biochemistry",
title = "Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin",
volume = "159",
pages = "89-95",
doi = "10.1016/j.jinorgbio.2016.02.034"
}

Nišavić, M., Masnikosa, R., Butorac, A., Perica, K., Rilak, A., Korićanac, L., Hozić, A., Petković, M.,& Cindrić, M.. (2016). Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin. in Journal of Inorganic Biochemistry, 159, 89-95.
https://doi.org/10.1016/j.jinorgbio.2016.02.034

Nišavić M, Masnikosa R, Butorac A, Perica K, Rilak A, Korićanac L, Hozić A, Petković M, Cindrić M. Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin. in Journal of Inorganic Biochemistry. 2016;159:89-95.
doi:10.1016/j.jinorgbio.2016.02.034 .

Nišavić, Marija, Masnikosa, Romana, Butorac, Ana, Perica, Kristina, Rilak, Ana, Korićanac, Lela, Hozić, Amela, Petković, Marijana, Cindrić, Mario, "Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin" in Journal of Inorganic Biochemistry, 159 (2016):89-95,
https://doi.org/10.1016/j.jinorgbio.2016.02.034 . .

TY  - JOUR
AU  - Popović, Iva A.
AU  - Nešić, Maja D.
AU  - Nišavić, Marija
AU  - Vranješ, Mila
AU  - Radetić, Tamara
AU  - Šaponjić, Zoran
AU  - Masnikosa, Romana
AU  - Petković, Marijana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/544
AB  - Nanostructured materials as substrates for surface-assisted laser desorption and ionization time-of-flight mass spectrometry have two main advantages such as small number of signals in the low mass range and salt tolerance. We have also previously observed decreased extent of undesired fragmentation of bipyridine-containing transition metal complexes in the presence of TiO2 nanoparticles (NPs). In this work, we extended our previous study and tested whether both TiO2 NPs and TiO2 prolate nanospheroids (PNSs) can be used as substrates for SALDI-MS of bipyridine-containing transition metal complexes and whether they tolerate intense laser and the presence of inorganic salts. TiO2 NPs (an average diameter of 5 nm) and PNSs (length: 40-50 nm, the lateral dimension: 14-16 nm) were characterized microscopically and their mass spectra were analyzed, as well as the spectra of bipyridine-containing Pt(II) and Ru(III) complexes. Our results show that seven times higher analyte signals can be obtained in the presence of PNSs compared to the signals acquired with NPs and three times higher compared to spectra acquired without a substrate. The presence of inorganic salt did not affect the spectra, which makes both TiO2 nanocrystals suitable for MS analysis of transition metal complexes in biological fluids. (C) 2015 Elsevier B.V. All rights reserved.
T2  - Materials Letters
T1  - Suitability of TiO2 nanoparticles and prolate nanospheroids for laser desorption/ionization mass spectrometric characterization of bipyridine-containing complexes
VL  - 150
SP  - 84
EP  - 88
DO  - 10.1016/j.matlet.2015.03.004
ER  - 

@article{
author = "Popović, Iva A. and Nešić, Maja D. and Nišavić, Marija and Vranješ, Mila and Radetić, Tamara and Šaponjić, Zoran and Masnikosa, Romana and Petković, Marijana",
year = "2015",
abstract = "Nanostructured materials as substrates for surface-assisted laser desorption and ionization time-of-flight mass spectrometry have two main advantages such as small number of signals in the low mass range and salt tolerance. We have also previously observed decreased extent of undesired fragmentation of bipyridine-containing transition metal complexes in the presence of TiO2 nanoparticles (NPs). In this work, we extended our previous study and tested whether both TiO2 NPs and TiO2 prolate nanospheroids (PNSs) can be used as substrates for SALDI-MS of bipyridine-containing transition metal complexes and whether they tolerate intense laser and the presence of inorganic salts. TiO2 NPs (an average diameter of 5 nm) and PNSs (length: 40-50 nm, the lateral dimension: 14-16 nm) were characterized microscopically and their mass spectra were analyzed, as well as the spectra of bipyridine-containing Pt(II) and Ru(III) complexes. Our results show that seven times higher analyte signals can be obtained in the presence of PNSs compared to the signals acquired with NPs and three times higher compared to spectra acquired without a substrate. The presence of inorganic salt did not affect the spectra, which makes both TiO2 nanocrystals suitable for MS analysis of transition metal complexes in biological fluids. (C) 2015 Elsevier B.V. All rights reserved.",
journal = "Materials Letters",
title = "Suitability of TiO2 nanoparticles and prolate nanospheroids for laser desorption/ionization mass spectrometric characterization of bipyridine-containing complexes",
volume = "150",
pages = "84-88",
doi = "10.1016/j.matlet.2015.03.004"
}

Popović, I. A., Nešić, M. D., Nišavić, M., Vranješ, M., Radetić, T., Šaponjić, Z., Masnikosa, R.,& Petković, M.. (2015). Suitability of TiO2 nanoparticles and prolate nanospheroids for laser desorption/ionization mass spectrometric characterization of bipyridine-containing complexes. in Materials Letters, 150, 84-88.
https://doi.org/10.1016/j.matlet.2015.03.004

Popović IA, Nešić MD, Nišavić M, Vranješ M, Radetić T, Šaponjić Z, Masnikosa R, Petković M. Suitability of TiO2 nanoparticles and prolate nanospheroids for laser desorption/ionization mass spectrometric characterization of bipyridine-containing complexes. in Materials Letters. 2015;150:84-88.
doi:10.1016/j.matlet.2015.03.004 .

Popović, Iva A., Nešić, Maja D., Nišavić, Marija, Vranješ, Mila, Radetić, Tamara, Šaponjić, Zoran, Masnikosa, Romana, Petković, Marijana, "Suitability of TiO2 nanoparticles and prolate nanospheroids for laser desorption/ionization mass spectrometric characterization of bipyridine-containing complexes" in Materials Letters, 150 (2015):84-88,
https://doi.org/10.1016/j.matlet.2015.03.004 . .

TY  - JOUR
AU  - Robajac, D.
AU  - Masnikosa, Romana
AU  - Mikovic, Z.
AU  - Mandie, V.
AU  - Nedić, Olgica
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/721
AB  - Placental insulin receptor (IR) and insulin-like growth factor receptors (IGFRs) are essential for fetal growth. We investigated structural changes of these receptors exposed to increased oxidative stress in mothers diagnosed with diabetes mellitus (DM) or preeclampsia (PE) complicated with intrauterine growth restriction. Increased amount of IR and decreased amounts of IGF1R and IGF2R were found in both pathologies, accompanied by significant elevation in protein carbonyls. When isolated receptors were examined, increased carbonylation of IR and IGF1R in PE placentas was detected, whereas the amounts of carbonylated IR and IGF1R were similar in DM and healthy placentas. Carbonylation status of IGF2R did not change due to pathology, confirming the detrimental role of primary structure and conformation in oxidative susceptibility. Ligand binding was similar in all three groups of samples and did not seem to be affected by receptor oxidation. Since babies delivered by mothers with PE were smaller than the referent population, increased carbonylation of receptors might have affected downstream receptor signaling post-ligand binding.
T2  - Free Radical Research
T1  - Oxidation of placental insulin and insulin-like growth factor receptors in mothers with diabetes mellitus or preeclampsia complicated with intrauterine growth restriction
VL  - 49
IS  - 8
SP  - 984
EP  - 989
DO  - 10.3109/10715762.2015.1020798
ER  - 

@article{
author = "Robajac, D. and Masnikosa, Romana and Mikovic, Z. and Mandie, V. and Nedić, Olgica",
year = "2015",
abstract = "Placental insulin receptor (IR) and insulin-like growth factor receptors (IGFRs) are essential for fetal growth. We investigated structural changes of these receptors exposed to increased oxidative stress in mothers diagnosed with diabetes mellitus (DM) or preeclampsia (PE) complicated with intrauterine growth restriction. Increased amount of IR and decreased amounts of IGF1R and IGF2R were found in both pathologies, accompanied by significant elevation in protein carbonyls. When isolated receptors were examined, increased carbonylation of IR and IGF1R in PE placentas was detected, whereas the amounts of carbonylated IR and IGF1R were similar in DM and healthy placentas. Carbonylation status of IGF2R did not change due to pathology, confirming the detrimental role of primary structure and conformation in oxidative susceptibility. Ligand binding was similar in all three groups of samples and did not seem to be affected by receptor oxidation. Since babies delivered by mothers with PE were smaller than the referent population, increased carbonylation of receptors might have affected downstream receptor signaling post-ligand binding.",
journal = "Free Radical Research",
title = "Oxidation of placental insulin and insulin-like growth factor receptors in mothers with diabetes mellitus or preeclampsia complicated with intrauterine growth restriction",
volume = "49",
number = "8",
pages = "984-989",
doi = "10.3109/10715762.2015.1020798"
}

Robajac, D., Masnikosa, R., Mikovic, Z., Mandie, V.,& Nedić, O.. (2015). Oxidation of placental insulin and insulin-like growth factor receptors in mothers with diabetes mellitus or preeclampsia complicated with intrauterine growth restriction. in Free Radical Research, 49(8), 984-989.
https://doi.org/10.3109/10715762.2015.1020798

Robajac D, Masnikosa R, Mikovic Z, Mandie V, Nedić O. Oxidation of placental insulin and insulin-like growth factor receptors in mothers with diabetes mellitus or preeclampsia complicated with intrauterine growth restriction. in Free Radical Research. 2015;49(8):984-989.
doi:10.3109/10715762.2015.1020798 .

Robajac, D., Masnikosa, Romana, Mikovic, Z., Mandie, V., Nedić, Olgica, "Oxidation of placental insulin and insulin-like growth factor receptors in mothers with diabetes mellitus or preeclampsia complicated with intrauterine growth restriction" in Free Radical Research, 49, no. 8 (2015):984-989,
https://doi.org/10.3109/10715762.2015.1020798 . .