Mačvanin, M., Klisić, A.,& Isenović, E. R.. (2024). Editorial: Molecular biomarkers of cardiometabolic disease. in Frontiers in Endocrinology, 15.
https://doi.org/10.3389/fendo.2024.1471571

Mačvanin M, Klisić A, Isenović ER. Editorial: Molecular biomarkers of cardiometabolic disease. in Frontiers in Endocrinology. 2024;15.
doi:10.3389/fendo.2024.1471571 .

Mačvanin, Mirjana, Klisić, Aleksandra, Isenović, Esma R., "Editorial: Molecular biomarkers of cardiometabolic disease" in Frontiers in Endocrinology, 15 (2024),
https://doi.org/10.3389/fendo.2024.1471571 . .

TY  - JOUR
AU  - Singh, Jaskaran
AU  - Khanna, Narendra N.
AU  - Rout, Ranjeet K.
AU  - Singh, Narpinder
AU  - Laird, John R.
AU  - Singh, Inder M.
AU  - Kalra, Mannudeep K.
AU  - Mantella, Laura E.
AU  - Johri, Amer M.
AU  - Isenović, Esma R.
AU  - Fouda, Mostafa M.
AU  - Saba, Luca
AU  - Fatemi, Mostafa
AU  - Suri, Jasjit S.
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13663
AB  - Due to the intricate relationship between the small non-coding ribonucleic acid (miRNA) sequences, the classification of miRNA species, namely Human, Gorilla, Rat, and Mouse is challenging. Previous methods are not robust and accurate. In this study, we present AtheroPoint’s GeneAI 3.0, a powerful, novel, and generalized method for extracting features from the fixed patterns of purines and pyrimidines in each miRNA sequence in ensemble paradigms in machine learning (EML) and convolutional neural network (CNN)-based deep learning (EDL) frameworks. GeneAI 3.0 utilized five conventional (Entropy, Dissimilarity, Energy, Homogeneity, and Contrast), and three contemporary (Shannon entropy, Hurst exponent, Fractal dimension) features, to generate a composite feature set from given miRNA sequences which were then passed into our ML and DL classification framework. A set of 11 new classifiers was designed consisting of 5 EML and 6 EDL for binary/multiclass classification. It was benchmarked against 9 solo ML (SML), 6 solo DL (SDL), 12 hybrid DL (HDL) models, resulting in a total of 11 + 27 = 38 models were designed. Four hypotheses were formulated and validated using explainable AI (XAI) as well as reliability/statistical tests. The order of the mean performance using accuracy (ACC)/area-under-the-curve (AUC) of the 24 DL classifiers was: EDL > HDL > SDL. The mean performance of EDL models with CNN layers was superior to that without CNN layers by 0.73%/0.92%. Mean performance of EML models was superior to SML models with improvements of ACC/AUC by 6.24%/6.46%. EDL models performed significantly better than EML models, with a mean increase in ACC/AUC of 7.09%/6.96%. The GeneAI 3.0 tool produced expected XAI feature plots, and the statistical tests showed significant p-values. Ensemble models with composite features are highly effective and generalized models for effectively classifying miRNA sequences.
T2  - Scientific Reports
T1  - GeneAI 3.0: powerful, novel, generalized hybrid and ensemble deep learning frameworks for miRNA species classification of stationary patterns from nucleotides
VL  - 14
IS  - 1
SP  - 7154
DO  - 10.1038/s41598-024-56786-9
ER  - 

@article{
author = "Singh, Jaskaran and Khanna, Narendra N. and Rout, Ranjeet K. and Singh, Narpinder and Laird, John R. and Singh, Inder M. and Kalra, Mannudeep K. and Mantella, Laura E. and Johri, Amer M. and Isenović, Esma R. and Fouda, Mostafa M. and Saba, Luca and Fatemi, Mostafa and Suri, Jasjit S.",
year = "2024",
abstract = "Due to the intricate relationship between the small non-coding ribonucleic acid (miRNA) sequences, the classification of miRNA species, namely Human, Gorilla, Rat, and Mouse is challenging. Previous methods are not robust and accurate. In this study, we present AtheroPoint’s GeneAI 3.0, a powerful, novel, and generalized method for extracting features from the fixed patterns of purines and pyrimidines in each miRNA sequence in ensemble paradigms in machine learning (EML) and convolutional neural network (CNN)-based deep learning (EDL) frameworks. GeneAI 3.0 utilized five conventional (Entropy, Dissimilarity, Energy, Homogeneity, and Contrast), and three contemporary (Shannon entropy, Hurst exponent, Fractal dimension) features, to generate a composite feature set from given miRNA sequences which were then passed into our ML and DL classification framework. A set of 11 new classifiers was designed consisting of 5 EML and 6 EDL for binary/multiclass classification. It was benchmarked against 9 solo ML (SML), 6 solo DL (SDL), 12 hybrid DL (HDL) models, resulting in a total of 11 + 27 = 38 models were designed. Four hypotheses were formulated and validated using explainable AI (XAI) as well as reliability/statistical tests. The order of the mean performance using accuracy (ACC)/area-under-the-curve (AUC) of the 24 DL classifiers was: EDL > HDL > SDL. The mean performance of EDL models with CNN layers was superior to that without CNN layers by 0.73%/0.92%. Mean performance of EML models was superior to SML models with improvements of ACC/AUC by 6.24%/6.46%. EDL models performed significantly better than EML models, with a mean increase in ACC/AUC of 7.09%/6.96%. The GeneAI 3.0 tool produced expected XAI feature plots, and the statistical tests showed significant p-values. Ensemble models with composite features are highly effective and generalized models for effectively classifying miRNA sequences.",
journal = "Scientific Reports",
title = "GeneAI 3.0: powerful, novel, generalized hybrid and ensemble deep learning frameworks for miRNA species classification of stationary patterns from nucleotides",
volume = "14",
number = "1",
pages = "7154",
doi = "10.1038/s41598-024-56786-9"
}

Singh, J., Khanna, N. N., Rout, R. K., Singh, N., Laird, J. R., Singh, I. M., Kalra, M. K., Mantella, L. E., Johri, A. M., Isenović, E. R., Fouda, M. M., Saba, L., Fatemi, M.,& Suri, J. S.. (2024). GeneAI 3.0: powerful, novel, generalized hybrid and ensemble deep learning frameworks for miRNA species classification of stationary patterns from nucleotides. in Scientific Reports, 14(1), 7154.
https://doi.org/10.1038/s41598-024-56786-9

Singh J, Khanna NN, Rout RK, Singh N, Laird JR, Singh IM, Kalra MK, Mantella LE, Johri AM, Isenović ER, Fouda MM, Saba L, Fatemi M, Suri JS. GeneAI 3.0: powerful, novel, generalized hybrid and ensemble deep learning frameworks for miRNA species classification of stationary patterns from nucleotides. in Scientific Reports. 2024;14(1):7154.
doi:10.1038/s41598-024-56786-9 .

Singh, Jaskaran, Khanna, Narendra N., Rout, Ranjeet K., Singh, Narpinder, Laird, John R., Singh, Inder M., Kalra, Mannudeep K., Mantella, Laura E., Johri, Amer M., Isenović, Esma R., Fouda, Mostafa M., Saba, Luca, Fatemi, Mostafa, Suri, Jasjit S., "GeneAI 3.0: powerful, novel, generalized hybrid and ensemble deep learning frameworks for miRNA species classification of stationary patterns from nucleotides" in Scientific Reports, 14, no. 1 (2024):7154,
https://doi.org/10.1038/s41598-024-56786-9 . .

TY  - JOUR
AU  - Nayak, Debasish Swapnesh Kumar
AU  - Mahapatra, Saswati
AU  - Routray, Sweta Padma
AU  - Sahoo, Swayamprabha
AU  - Sahoo, Santanu Kumar
AU  - Fouda, Mostafa M.
AU  - Singh, Narpinder
AU  - Isenović, Esma R.
AU  - Saba, Luca
AU  - Suri, Jasjit S.
AU  - Swarnkar, Tripti
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13664
AB  - Background: There are several antibiotic resistance genes (ARG) for the Escherichia coli (E. coli) bacteria that cause urinary tract infections (UTI), and it is therefore important to identify these ARG. Artificial Intelligence (AI) has been used previously in the field of gene expression data, but never adopted for the detection and classification of bacterial ARG. We hypothesize, if the data is correctly conferred, right features are selected, and Deep Learning (DL) classification models are optimized, then (i) non-linear DL models would perform better than Machine Learning (ML) models, (ii) leads to higher accuracy, (iii) can identify the hub genes, and, (iv) can identify gene pathways accurately. We have therefore designed aiGeneR, the first of its kind system that uses DL-based models to identify ARG in E. coli in gene expression data. Methodology: The aiGeneR consists of a tandem connection of quality control embedded with feature extraction and AI-based classification of ARG. We adopted a cross-validation approach to evaluate the performance of aiGeneR using accuracy, precision, recall, and F1-score. Further, we analyzed the effect of sample size ensuring generalization of models and compare against the power analysis. The aiGeneR was validated scientifically and biologically for hub genes and pathways. We benchmarked aiGeneR against two linear and two other non-linear AI models. Results: The aiGeneR identifies tetM (an ARG) and showed an accuracy of 93% with area under the curve (AUC) of 0.99 (p < 0.05). The mean accuracy of non-linear models was 22% higher compared to linear models. We scientifically and biologically validated the aiGeneR. Conclusions: aiGeneR successfully detected the E. coli genes validating our four hypotheses.
T2  - Frontiers in Bioscience-Landmark
T1  - aiGeneR 1.0: An Artificial Intelligence Technique for the Revelation of Informative and Antibiotic Resistant Genes in Escherichia coli
VL  - 29
IS  - 2
SP  - 82
DO  - 10.31083/j.fbl2902082
ER  - 

@article{
author = "Nayak, Debasish Swapnesh Kumar and Mahapatra, Saswati and Routray, Sweta Padma and Sahoo, Swayamprabha and Sahoo, Santanu Kumar and Fouda, Mostafa M. and Singh, Narpinder and Isenović, Esma R. and Saba, Luca and Suri, Jasjit S. and Swarnkar, Tripti",
year = "2024",
abstract = "Background: There are several antibiotic resistance genes (ARG) for the Escherichia coli (E. coli) bacteria that cause urinary tract infections (UTI), and it is therefore important to identify these ARG. Artificial Intelligence (AI) has been used previously in the field of gene expression data, but never adopted for the detection and classification of bacterial ARG. We hypothesize, if the data is correctly conferred, right features are selected, and Deep Learning (DL) classification models are optimized, then (i) non-linear DL models would perform better than Machine Learning (ML) models, (ii) leads to higher accuracy, (iii) can identify the hub genes, and, (iv) can identify gene pathways accurately. We have therefore designed aiGeneR, the first of its kind system that uses DL-based models to identify ARG in E. coli in gene expression data. Methodology: The aiGeneR consists of a tandem connection of quality control embedded with feature extraction and AI-based classification of ARG. We adopted a cross-validation approach to evaluate the performance of aiGeneR using accuracy, precision, recall, and F1-score. Further, we analyzed the effect of sample size ensuring generalization of models and compare against the power analysis. The aiGeneR was validated scientifically and biologically for hub genes and pathways. We benchmarked aiGeneR against two linear and two other non-linear AI models. Results: The aiGeneR identifies tetM (an ARG) and showed an accuracy of 93% with area under the curve (AUC) of 0.99 (p < 0.05). The mean accuracy of non-linear models was 22% higher compared to linear models. We scientifically and biologically validated the aiGeneR. Conclusions: aiGeneR successfully detected the E. coli genes validating our four hypotheses.",
journal = "Frontiers in Bioscience-Landmark",
title = "aiGeneR 1.0: An Artificial Intelligence Technique for the Revelation of Informative and Antibiotic Resistant Genes in Escherichia coli",
volume = "29",
number = "2",
pages = "82",
doi = "10.31083/j.fbl2902082"
}

Nayak, D. S. K., Mahapatra, S., Routray, S. P., Sahoo, S., Sahoo, S. K., Fouda, M. M., Singh, N., Isenović, E. R., Saba, L., Suri, J. S.,& Swarnkar, T.. (2024). aiGeneR 1.0: An Artificial Intelligence Technique for the Revelation of Informative and Antibiotic Resistant Genes in Escherichia coli. in Frontiers in Bioscience-Landmark, 29(2), 82.
https://doi.org/10.31083/j.fbl2902082

Nayak DSK, Mahapatra S, Routray SP, Sahoo S, Sahoo SK, Fouda MM, Singh N, Isenović ER, Saba L, Suri JS, Swarnkar T. aiGeneR 1.0: An Artificial Intelligence Technique for the Revelation of Informative and Antibiotic Resistant Genes in Escherichia coli. in Frontiers in Bioscience-Landmark. 2024;29(2):82.
doi:10.31083/j.fbl2902082 .

Nayak, Debasish Swapnesh Kumar, Mahapatra, Saswati, Routray, Sweta Padma, Sahoo, Swayamprabha, Sahoo, Santanu Kumar, Fouda, Mostafa M., Singh, Narpinder, Isenović, Esma R., Saba, Luca, Suri, Jasjit S., Swarnkar, Tripti, "aiGeneR 1.0: An Artificial Intelligence Technique for the Revelation of Informative and Antibiotic Resistant Genes in Escherichia coli" in Frontiers in Bioscience-Landmark, 29, no. 2 (2024):82,
https://doi.org/10.31083/j.fbl2902082 . .

TY  - JOUR
AU  - Banjac, Katarina
AU  - Obradović, MIlan
AU  - Zafirović, Sonja
AU  - Isenović, Esma R.
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13489
AB  - Introduction: Insulin-like growth factor-1 (IGF-1) promotes survival and inhibits cardiac autophagy disruption. Methods: Male Wistar rats were treated with IGF-1 (50 μg/kg), and 24 h after injection hearts were excised. The level of interaction between Beclin-1 and the α1 subunit of sodium/potassium-adenosine triphosphates (Na+/K+-ATPase), and phosphorylated forms of IGF-1 receptor/insulin receptor (IGF-1R/IR), forkhead box protein O1 (FOXO1) and AMP-activated protein kinase (AMPK) were measured. Results: The results indicate that IGF-1 decreased Beclin-1’s association with Na+/K+-ATPase (p < 0.05), increased IGF-1R/IR and FOXO1 phosphorylation (p < 0.05), and decreased AMPK phosphorylation (p < 0.01) in rats’ hearts. Conclusions: The new IGF-1 therapy may control autosis and minimize cardiomyocyte mortality.
T2  - Archives of Medical Science
T1  - Insulin-like growth factor-1 reduces cardiac autosis through decreasing AMPK/FOXO1 signaling and Na+/K+-ATPase-Beclin-1 interaction
VL  - 20
IS  - 3
SP  - 1011
EP  - 1015
DO  - 10.5114/aoms/177618
ER  - 

@article{
author = "Banjac, Katarina and Obradović, MIlan and Zafirović, Sonja and Isenović, Esma R.",
year = "2024",
abstract = "Introduction: Insulin-like growth factor-1 (IGF-1) promotes survival and inhibits cardiac autophagy disruption. Methods: Male Wistar rats were treated with IGF-1 (50 μg/kg), and 24 h after injection hearts were excised. The level of interaction between Beclin-1 and the α1 subunit of sodium/potassium-adenosine triphosphates (Na+/K+-ATPase), and phosphorylated forms of IGF-1 receptor/insulin receptor (IGF-1R/IR), forkhead box protein O1 (FOXO1) and AMP-activated protein kinase (AMPK) were measured. Results: The results indicate that IGF-1 decreased Beclin-1’s association with Na+/K+-ATPase (p < 0.05), increased IGF-1R/IR and FOXO1 phosphorylation (p < 0.05), and decreased AMPK phosphorylation (p < 0.01) in rats’ hearts. Conclusions: The new IGF-1 therapy may control autosis and minimize cardiomyocyte mortality.",
journal = "Archives of Medical Science",
title = "Insulin-like growth factor-1 reduces cardiac autosis through decreasing AMPK/FOXO1 signaling and Na+/K+-ATPase-Beclin-1 interaction",
volume = "20",
number = "3",
pages = "1011-1015",
doi = "10.5114/aoms/177618"
}

Banjac, K., Obradović, M., Zafirović, S.,& Isenović, E. R.. (2024). Insulin-like growth factor-1 reduces cardiac autosis through decreasing AMPK/FOXO1 signaling and Na+/K+-ATPase-Beclin-1 interaction. in Archives of Medical Science, 20(3), 1011-1015.
https://doi.org/10.5114/aoms/177618

Banjac K, Obradović M, Zafirović S, Isenović ER. Insulin-like growth factor-1 reduces cardiac autosis through decreasing AMPK/FOXO1 signaling and Na+/K+-ATPase-Beclin-1 interaction. in Archives of Medical Science. 2024;20(3):1011-1015.
doi:10.5114/aoms/177618 .

Banjac, Katarina, Obradović, MIlan, Zafirović, Sonja, Isenović, Esma R., "Insulin-like growth factor-1 reduces cardiac autosis through decreasing AMPK/FOXO1 signaling and Na+/K+-ATPase-Beclin-1 interaction" in Archives of Medical Science, 20, no. 3 (2024):1011-1015,
https://doi.org/10.5114/aoms/177618 . .

TY  - JOUR
AU  - Banjac, Katarina
AU  - Obradović, Milan
AU  - Zafirović, Sonja
AU  - Essack, Magbubah
AU  - Gluvić, Zoran
AU  - Šunderić, Miloš
AU  - Nedić, Olgica
AU  - Isenović, Esma R.
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13184
AB  - Background We previously demonstrated that insulin-like growth factor-1 (IGF-1) regulates sodium/potassium adenosine triphosphatase (Na+/K+-ATPase) in vascular smooth muscle cells (VSMC) via phosphatidylinositol-3 kinase (PI3K). Taking into account that others’ work show that IGF-1 activates the PI3K/protein kinase B (Akt) signaling pathway in many different cells, we here further questioned if the Akt/mammalian target of rapamycin (mTOR)/ribosomal protein p70 S6 kinase (S6K) pathway stimulates Na+/K+-ATPase, an essential protein for maintaining normal heart function. Methods and results There were 14 adult male Wistar rats, half of whom received bolus injections of IGF-1 (50 μg/kg) for 24 h. We evaluated cardiac Na+/K+-ATPase expression, activity, and serum IGF-1 levels. Additionally, we examined the phosphorylated forms of the following proteins: insulin receptor substrate (IRS), phosphoinositide-dependent kinase-1 (PDK-1), Akt, mTOR, S6K, and α subunit of Na+/K+-ATPase. Additionally, the mRNA expression of the Na+/K+-ATPase α1 subunit was evaluated. Treatment with IGF-1 increases levels of serum IGF-1 and stimulates Na+/K+-ATPase activity, phosphorylation of α subunit of Na+/K+-ATPase on Ser23, and protein expression of α2 subunit. Furthermore, IGF-1 treatment increased phosphorylation of IRS-1 on Tyr1222, Akt on Ser473, PDK-1 on Ser241, mTOR on Ser2481 and Ser2448, and S6K on Thr421/Ser424. The concentration of IGF-1 in serum positively correlates with Na+/K+-ATPase activity and the phosphorylated form of mTOR (Ser2448), while Na+/K+-ATPase activity positively correlates with the phosphorylated form of IRS-1 (Tyr1222) and mTOR (Ser2448). Conclusion These results indicate that the Akt/mTOR/S6K signalling pathway may be involved in the IGF-1 regulating cardiac Na+/K+-ATPase expression and activity
T2  - Molecular Biology Reports
T1  - The involvement of Akt, mTOR, and S6K in the in vivo effect of IGF-1 on the regulation of rat cardiac Na+/K+-ATPase
VL  - 51
IS  - 1
DO  - 10.1007/s11033-024-09451-3
ER  - 

@article{
author = "Banjac, Katarina and Obradović, Milan and Zafirović, Sonja and Essack, Magbubah and Gluvić, Zoran and Šunderić, Miloš and Nedić, Olgica and Isenović, Esma R.",
year = "2024",
abstract = "Background We previously demonstrated that insulin-like growth factor-1 (IGF-1) regulates sodium/potassium adenosine triphosphatase (Na+/K+-ATPase) in vascular smooth muscle cells (VSMC) via phosphatidylinositol-3 kinase (PI3K). Taking into account that others’ work show that IGF-1 activates the PI3K/protein kinase B (Akt) signaling pathway in many different cells, we here further questioned if the Akt/mammalian target of rapamycin (mTOR)/ribosomal protein p70 S6 kinase (S6K) pathway stimulates Na+/K+-ATPase, an essential protein for maintaining normal heart function. Methods and results There were 14 adult male Wistar rats, half of whom received bolus injections of IGF-1 (50 μg/kg) for 24 h. We evaluated cardiac Na+/K+-ATPase expression, activity, and serum IGF-1 levels. Additionally, we examined the phosphorylated forms of the following proteins: insulin receptor substrate (IRS), phosphoinositide-dependent kinase-1 (PDK-1), Akt, mTOR, S6K, and α subunit of Na+/K+-ATPase. Additionally, the mRNA expression of the Na+/K+-ATPase α1 subunit was evaluated. Treatment with IGF-1 increases levels of serum IGF-1 and stimulates Na+/K+-ATPase activity, phosphorylation of α subunit of Na+/K+-ATPase on Ser23, and protein expression of α2 subunit. Furthermore, IGF-1 treatment increased phosphorylation of IRS-1 on Tyr1222, Akt on Ser473, PDK-1 on Ser241, mTOR on Ser2481 and Ser2448, and S6K on Thr421/Ser424. The concentration of IGF-1 in serum positively correlates with Na+/K+-ATPase activity and the phosphorylated form of mTOR (Ser2448), while Na+/K+-ATPase activity positively correlates with the phosphorylated form of IRS-1 (Tyr1222) and mTOR (Ser2448). Conclusion These results indicate that the Akt/mTOR/S6K signalling pathway may be involved in the IGF-1 regulating cardiac Na+/K+-ATPase expression and activity",
journal = "Molecular Biology Reports",
title = "The involvement of Akt, mTOR, and S6K in the in vivo effect of IGF-1 on the regulation of rat cardiac Na+/K+-ATPase",
volume = "51",
number = "1",
doi = "10.1007/s11033-024-09451-3"
}

Banjac, K., Obradović, M., Zafirović, S., Essack, M., Gluvić, Z., Šunderić, M., Nedić, O.,& Isenović, E. R.. (2024). The involvement of Akt, mTOR, and S6K in the in vivo effect of IGF-1 on the regulation of rat cardiac Na+/K+-ATPase. in Molecular Biology Reports, 51(1).
https://doi.org/10.1007/s11033-024-09451-3

Banjac K, Obradović M, Zafirović S, Essack M, Gluvić Z, Šunderić M, Nedić O, Isenović ER. The involvement of Akt, mTOR, and S6K in the in vivo effect of IGF-1 on the regulation of rat cardiac Na+/K+-ATPase. in Molecular Biology Reports. 2024;51(1).
doi:10.1007/s11033-024-09451-3 .

Banjac, Katarina, Obradović, Milan, Zafirović, Sonja, Essack, Magbubah, Gluvić, Zoran, Šunderić, Miloš, Nedić, Olgica, Isenović, Esma R., "The involvement of Akt, mTOR, and S6K in the in vivo effect of IGF-1 on the regulation of rat cardiac Na+/K+-ATPase" in Molecular Biology Reports, 51, no. 1 (2024),
https://doi.org/10.1007/s11033-024-09451-3 . .

TY  - JOUR
AU  - Smiljanić, Katarina
AU  - Obradović, Milan M.
AU  - Jovanović, Aleksandra
AU  - Đorđević, Jelena
AU  - Dobutović, Branislava
AU  - Jevremović, Danimir
AU  - Marche, Pierre
AU  - Isenović, Esma R.
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13373
AB  - The Editors-in-Chief have retracted this article. After publication, concerns were raised regarding image irregularities in the Figures. Specifically,

    In Figs. 1a and 6a, the p-EGFR lanes 1 and 2 appear highly similar, but the t-EGFR blots are different.

    In Figs. 2a and 3a, the t-EGFR panels appear highly similar.

    Fig. 2a and 3a t-EGFR lanes 3 and 4 appear highly similar to Fig. 4a t-EGFR lanes 1 and 2 (flipped horizontally).

    Fig. 4b p-ERK lanes 1, 2 and 4 appear highly similar to Fig. 6b p-ERK lanes 3, 2 and 4, respectively (flipped horizontally).

    In Figs. 5b and 7b, the t-EGFR panels appear highly similar.

The Editors-in-Chief therefore no longer have confidence in the presented data.

Katarina Smiljanic and Esma R. Isenovic do not agree to this retraction. Pierre Marche is deceased. Milan Obradovic, Aleksandra Jovanovic, Jelena Djordjevic, Branislava Dobutovic and Danimir Jevremovic have not responded to any correspondence from the publisher about this retraction.
T2  - Molecular and Cellular Biochemistry
T1  - Retraction Note: Thrombin stimulates VSMC proliferation through an EGFR-dependent pathway: involvement of MMP-2
DO  - 10.1007/s11010-024-05016-x
ER  - 

@article{
author = "Smiljanić, Katarina and Obradović, Milan M. and Jovanović, Aleksandra and Đorđević, Jelena and Dobutović, Branislava and Jevremović, Danimir and Marche, Pierre and Isenović, Esma R.",
year = "2024",
abstract = "The Editors-in-Chief have retracted this article. After publication, concerns were raised regarding image irregularities in the Figures. Specifically,

    In Figs. 1a and 6a, the p-EGFR lanes 1 and 2 appear highly similar, but the t-EGFR blots are different.

    In Figs. 2a and 3a, the t-EGFR panels appear highly similar.

    Fig. 2a and 3a t-EGFR lanes 3 and 4 appear highly similar to Fig. 4a t-EGFR lanes 1 and 2 (flipped horizontally).

    Fig. 4b p-ERK lanes 1, 2 and 4 appear highly similar to Fig. 6b p-ERK lanes 3, 2 and 4, respectively (flipped horizontally).

    In Figs. 5b and 7b, the t-EGFR panels appear highly similar.

The Editors-in-Chief therefore no longer have confidence in the presented data.

Katarina Smiljanic and Esma R. Isenovic do not agree to this retraction. Pierre Marche is deceased. Milan Obradovic, Aleksandra Jovanovic, Jelena Djordjevic, Branislava Dobutovic and Danimir Jevremovic have not responded to any correspondence from the publisher about this retraction.",
journal = "Molecular and Cellular Biochemistry",
title = "Retraction Note: Thrombin stimulates VSMC proliferation through an EGFR-dependent pathway: involvement of MMP-2",
doi = "10.1007/s11010-024-05016-x"
}

Smiljanić, K., Obradović, M. M., Jovanović, A., Đorđević, J., Dobutović, B., Jevremović, D., Marche, P.,& Isenović, E. R.. (2024). Retraction Note: Thrombin stimulates VSMC proliferation through an EGFR-dependent pathway: involvement of MMP-2. in Molecular and Cellular Biochemistry.
https://doi.org/10.1007/s11010-024-05016-x

Smiljanić K, Obradović MM, Jovanović A, Đorđević J, Dobutović B, Jevremović D, Marche P, Isenović ER. Retraction Note: Thrombin stimulates VSMC proliferation through an EGFR-dependent pathway: involvement of MMP-2. in Molecular and Cellular Biochemistry. 2024;.
doi:10.1007/s11010-024-05016-x .

Smiljanić, Katarina, Obradović, Milan M., Jovanović, Aleksandra, Đorđević, Jelena, Dobutović, Branislava, Jevremović, Danimir, Marche, Pierre, Isenović, Esma R., "Retraction Note: Thrombin stimulates VSMC proliferation through an EGFR-dependent pathway: involvement of MMP-2" in Molecular and Cellular Biochemistry (2024),
https://doi.org/10.1007/s11010-024-05016-x . .

TY  - JOUR
AU  - Gluvić, Zoran
AU  - Obradović, Milan M.
AU  - Manojlović, Mia
AU  - Vincenza Giglio, Rosaria
AU  - Maria Patti, Angelo
AU  - Ciaccio, Marcello
AU  - Suri, Jasjit S.
AU  - Rizzo, Manfredi
AU  - Isenović, Esma R.
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13439
AB  - Polymetabolic syndrome achieved pandemic proportions and dramatically influenced public health systems functioning worldwide. Chronic vascular complications are the major contributors to increased morbidity, disability, and mortality rates in diabetes patients. Nitric oxide (NO) is among the most important vascular bed function regulators. However, NO homeostasis is significantly deranged in pathological conditions. Additionally, different hormones directly or indirectly affect NO production and activity and subsequently act on vascular physiology. In this paper, we summarize the recent literature data related to the effects of insulin, estradiol, insulin-like growth factor-1, ghrelin, angiotensin II and irisin on the NO regulation in physiological and diabetes circumstances.
T2  - Molecular and Cellular Endocrinology
T1  - Impact of different hormones on the regulation of nitric oxide in diabetes
VL  - 592
SP  - 112325
DO  - 10.1016/j.mce.2024.112325
ER  - 

@article{
author = "Gluvić, Zoran and Obradović, Milan M. and Manojlović, Mia and Vincenza Giglio, Rosaria and Maria Patti, Angelo and Ciaccio, Marcello and Suri, Jasjit S. and Rizzo, Manfredi and Isenović, Esma R.",
year = "2024",
abstract = "Polymetabolic syndrome achieved pandemic proportions and dramatically influenced public health systems functioning worldwide. Chronic vascular complications are the major contributors to increased morbidity, disability, and mortality rates in diabetes patients. Nitric oxide (NO) is among the most important vascular bed function regulators. However, NO homeostasis is significantly deranged in pathological conditions. Additionally, different hormones directly or indirectly affect NO production and activity and subsequently act on vascular physiology. In this paper, we summarize the recent literature data related to the effects of insulin, estradiol, insulin-like growth factor-1, ghrelin, angiotensin II and irisin on the NO regulation in physiological and diabetes circumstances.",
journal = "Molecular and Cellular Endocrinology",
title = "Impact of different hormones on the regulation of nitric oxide in diabetes",
volume = "592",
pages = "112325",
doi = "10.1016/j.mce.2024.112325"
}

Gluvić, Z., Obradović, M. M., Manojlović, M., Vincenza Giglio, R., Maria Patti, A., Ciaccio, M., Suri, J. S., Rizzo, M.,& Isenović, E. R.. (2024). Impact of different hormones on the regulation of nitric oxide in diabetes. in Molecular and Cellular Endocrinology, 592, 112325.
https://doi.org/10.1016/j.mce.2024.112325

Gluvić Z, Obradović MM, Manojlović M, Vincenza Giglio R, Maria Patti A, Ciaccio M, Suri JS, Rizzo M, Isenović ER. Impact of different hormones on the regulation of nitric oxide in diabetes. in Molecular and Cellular Endocrinology. 2024;592:112325.
doi:10.1016/j.mce.2024.112325 .

Gluvić, Zoran, Obradović, Milan M., Manojlović, Mia, Vincenza Giglio, Rosaria, Maria Patti, Angelo, Ciaccio, Marcello, Suri, Jasjit S., Rizzo, Manfredi, Isenović, Esma R., "Impact of different hormones on the regulation of nitric oxide in diabetes" in Molecular and Cellular Endocrinology, 592 (2024):112325,
https://doi.org/10.1016/j.mce.2024.112325 . .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Patoulias, Dimitrios
AU  - Isenović, Esma R.
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13182
T2  - Frontiers in Endocrinology
T1  - Editorial: Oxidative stress and inflammation in cardiometabolic disorders
VL  - 15
DO  - 10.3389/fendo.2024.1397836
ER  - 

@article{
author = "Klisić, Aleksandra and Patoulias, Dimitrios and Isenović, Esma R.",
year = "2024",
journal = "Frontiers in Endocrinology",
title = "Editorial: Oxidative stress and inflammation in cardiometabolic disorders",
volume = "15",
doi = "10.3389/fendo.2024.1397836"
}

Klisić, A., Patoulias, D.,& Isenović, E. R.. (2024). Editorial: Oxidative stress and inflammation in cardiometabolic disorders. in Frontiers in Endocrinology, 15.
https://doi.org/10.3389/fendo.2024.1397836

Klisić A, Patoulias D, Isenović ER. Editorial: Oxidative stress and inflammation in cardiometabolic disorders. in Frontiers in Endocrinology. 2024;15.
doi:10.3389/fendo.2024.1397836 .

Klisić, Aleksandra, Patoulias, Dimitrios, Isenović, Esma R., "Editorial: Oxidative stress and inflammation in cardiometabolic disorders" in Frontiers in Endocrinology, 15 (2024),
https://doi.org/10.3389/fendo.2024.1397836 . .

TY  - JOUR
AU  - Lukić, Nikola
AU  - Mačvanin, Mirjana T.
AU  - Gluvić, Zoran
AU  - Rizzo, Manfredi
AU  - Radak, Đorđe
AU  - Suri, Jasjit S.
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12022
AB  - Type 2 diabetes mellitus (T2DM) has become a worldwide concern in recent years, primarily in highly developed Western societies. T2DM causes systemic complications, such as atherosclerotic heart disease, ischemic stroke, peripheral artery disease, kidney failure, and diabetes-related maculopathy and retinopathy. The growing number of T2DM patients and the treatment of long-term T2DM-related complications pressurize and exhaust public healthcare systems. As a result, strategies for combating T2DM and developing novel drugs are critical global public health requirements. Aside from preventive measures, which are still the most effective way to prevent T2DM, novel and highly effective therapies are emerging. In the spotlight of next-generation T2DM treatment, sodium-glucose co-transporter 2 (SGLT-2) inhibitors are promoted as the most efficient perspective therapy. SGLT-2 inhibitors (SGLT2i) include phlorizin derivatives, such as canagliflozin, dapagliflozin, empagliflozin, and ertugliflozin. SGLT-2, along with SGLT-1, is a member of the SGLT family of proteins that play a role in glucose absorption via active transport mediated by Na+ /K+ ATPase. SGLT-2 is only found in the kidney, specifically the proximal tubule, and is responsible for more than 90% glucose absorption. Inhibition of SGLT-2 reduces glucose absorption, and consequently increases urinary glucose excretion, decreasing blood glucose levels. Thus, the inhibition of SGLT-2 activity ultimately alleviates T2DM-related symptoms and prevents or delays systemic T2DM-associated chronic complications. This review aimed to provide a more detailed understanding of the effects of SGLT2i responsible for the acute improvement in blood glucose regulation, a prerequisite for T2DM-associated cardiovascular complications control.
T2  - Current Medicinal Chemistry
T1  - SGLT-2 Inhibitors: The Next-generation Treatment for Type 2 Diabetes Mellitus
VL  - 31
SP  - 4781
EP  - 4806
DO  - 10.2174/0109298673251493231011192520
ER  - 

@article{
author = "Lukić, Nikola and Mačvanin, Mirjana T. and Gluvić, Zoran and Rizzo, Manfredi and Radak, Đorđe and Suri, Jasjit S. and Isenović, Esma R.",
year = "2023",
abstract = "Type 2 diabetes mellitus (T2DM) has become a worldwide concern in recent years, primarily in highly developed Western societies. T2DM causes systemic complications, such as atherosclerotic heart disease, ischemic stroke, peripheral artery disease, kidney failure, and diabetes-related maculopathy and retinopathy. The growing number of T2DM patients and the treatment of long-term T2DM-related complications pressurize and exhaust public healthcare systems. As a result, strategies for combating T2DM and developing novel drugs are critical global public health requirements. Aside from preventive measures, which are still the most effective way to prevent T2DM, novel and highly effective therapies are emerging. In the spotlight of next-generation T2DM treatment, sodium-glucose co-transporter 2 (SGLT-2) inhibitors are promoted as the most efficient perspective therapy. SGLT-2 inhibitors (SGLT2i) include phlorizin derivatives, such as canagliflozin, dapagliflozin, empagliflozin, and ertugliflozin. SGLT-2, along with SGLT-1, is a member of the SGLT family of proteins that play a role in glucose absorption via active transport mediated by Na+ /K+ ATPase. SGLT-2 is only found in the kidney, specifically the proximal tubule, and is responsible for more than 90% glucose absorption. Inhibition of SGLT-2 reduces glucose absorption, and consequently increases urinary glucose excretion, decreasing blood glucose levels. Thus, the inhibition of SGLT-2 activity ultimately alleviates T2DM-related symptoms and prevents or delays systemic T2DM-associated chronic complications. This review aimed to provide a more detailed understanding of the effects of SGLT2i responsible for the acute improvement in blood glucose regulation, a prerequisite for T2DM-associated cardiovascular complications control.",
journal = "Current Medicinal Chemistry",
title = "SGLT-2 Inhibitors: The Next-generation Treatment for Type 2 Diabetes Mellitus",
volume = "31",
pages = "4781-4806",
doi = "10.2174/0109298673251493231011192520"
}

Lukić, N., Mačvanin, M. T., Gluvić, Z., Rizzo, M., Radak, Đ., Suri, J. S.,& Isenović, E. R.. (2023). SGLT-2 Inhibitors: The Next-generation Treatment for Type 2 Diabetes Mellitus. in Current Medicinal Chemistry, 31, 4781-4806.
https://doi.org/10.2174/0109298673251493231011192520

Lukić N, Mačvanin MT, Gluvić Z, Rizzo M, Radak Đ, Suri JS, Isenović ER. SGLT-2 Inhibitors: The Next-generation Treatment for Type 2 Diabetes Mellitus. in Current Medicinal Chemistry. 2023;31:4781-4806.
doi:10.2174/0109298673251493231011192520 .

Lukić, Nikola, Mačvanin, Mirjana T., Gluvić, Zoran, Rizzo, Manfredi, Radak, Đorđe, Suri, Jasjit S., Isenović, Esma R., "SGLT-2 Inhibitors: The Next-generation Treatment for Type 2 Diabetes Mellitus" in Current Medicinal Chemistry, 31 (2023):4781-4806,
https://doi.org/10.2174/0109298673251493231011192520 . .

TY  - JOUR
AU  - Mačvanin, Mirjana
AU  - Zafirović, Sonja
AU  - Obradović, Milan M.
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10687
T2  - Frontiers in Endocrinology
T1  - Editorial: Non-coding RNA in diabetes and cardiovascular diseases
VL  - 14
DO  - 10.3389/fendo.2023.1149857
ER  - 

@article{
author = "Mačvanin, Mirjana and Zafirović, Sonja and Obradović, Milan M. and Isenović, Esma R.",
year = "2023",
journal = "Frontiers in Endocrinology",
title = "Editorial: Non-coding RNA in diabetes and cardiovascular diseases",
volume = "14",
doi = "10.3389/fendo.2023.1149857"
}

Mačvanin, M., Zafirović, S., Obradović, M. M.,& Isenović, E. R.. (2023). Editorial: Non-coding RNA in diabetes and cardiovascular diseases. in Frontiers in Endocrinology, 14.
https://doi.org/10.3389/fendo.2023.1149857

Mačvanin M, Zafirović S, Obradović MM, Isenović ER. Editorial: Non-coding RNA in diabetes and cardiovascular diseases. in Frontiers in Endocrinology. 2023;14.
doi:10.3389/fendo.2023.1149857 .

Mačvanin, Mirjana, Zafirović, Sonja, Obradović, Milan M., Isenović, Esma R., "Editorial: Non-coding RNA in diabetes and cardiovascular diseases" in Frontiers in Endocrinology, 14 (2023),
https://doi.org/10.3389/fendo.2023.1149857 . .

TY  - JOUR
AU  - Panić, Anastasija
AU  - Sudar-Milovanović, Emina
AU  - Stanimirović, Julijana
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Soskić, Sanja S.
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10744
AB  - The liver is an organ in which many oxidative processes occur and represents an important target of oxidative stress (OxS). Under physiological conditions of normal mitochondrial homeostasis, hepatocytes effectively remove reactive oxygen species (ROS) by enabling metabolic adaptations and through the antioxidant defence system mechanisms. However, obesity-induced lipid accumulation in the hepatocytes causes significantly elevated production of ROS, reduces oxidative capacity, and increases oxidative stress (OxS). In men, compared with premenopausal women, the development of insulin resistance (IR) and non-alcoholic fatty liver disease (NAFLD) in obesity are more prevalent, where oestradiol (E2), the most potent female sex steroid, is proposed as the main culprit. Exogenous oestradiol (E2) administration exerts beneficial effects on antioxidant properties, restores total plasma antioxidant capacity and decreases biomarkers of OxS in ovariectomized animal models. Thus, we hypothesized that E2 treatment in states of obesity could have beneficial effects against OxS in the obese male's liver. We assumed that E2 could directly affect the level of OxS by increasing the level/activity of the AOS enzymes, particularly SOD1, SOD2, GPx, and CAT, in obese males' livers. In addition, we assumed that the level of malondialdehyde (MDA) and protein carbonyl content (PCC) in obese males' livers would be reduced after E2 treatment as a result of E2 inhibitory effect on lipid peroxidation and protein oxidation, respectively. To test our hypothesis, we used the liver of a high-fat (HF) diet-fed male Wistar rats as an animal model of obesity, treated with E2 intraperitoneally (40 μg/kg). Preliminary results from this study support our hypothesis that E2 increases liver protein expression of AOS enzymes: SOD1, GPx, and CAT, in control and HF male rats compared with their respective controls. The protein level of SOD2 and CAT activity was increased in HF treated with E2 compared with non-treated HF rats. Moreover, as we expected, E2 administration significantly decreased the MDA level in both E2-treated groups compared to their controls, while the PCC level was significantly decreased in HF treated group compared with untreated HF rats. In conclusion, the preliminary results we obtained in this study indicate that E2 administration can effectively inhibit the OxS-related processes in the liver in HF diet-induced obesity by increasing AOS enzymes levels and CAT activity, and also by decreasing levels of MDA and PCC. A consequence of our hypothesis is that treatment with E2 may be an innovative way to improve obesity-related liver disease prevention and healing. © 2023 Elsevier Ltd
T2  - Medical Hypotheses
T1  - Does oestradiol treatment alleviate obesity-induced oxidative stress in the male liver?
VL  - 174
SP  - 111049
DO  - 10.1016/j.mehy.2023.111049
ER  - 

@article{
author = "Panić, Anastasija and Sudar-Milovanović, Emina and Stanimirović, Julijana and Obradović, Milan M. and Zafirović, Sonja and Soskić, Sanja S. and Isenović, Esma R.",
year = "2023",
abstract = "The liver is an organ in which many oxidative processes occur and represents an important target of oxidative stress (OxS). Under physiological conditions of normal mitochondrial homeostasis, hepatocytes effectively remove reactive oxygen species (ROS) by enabling metabolic adaptations and through the antioxidant defence system mechanisms. However, obesity-induced lipid accumulation in the hepatocytes causes significantly elevated production of ROS, reduces oxidative capacity, and increases oxidative stress (OxS). In men, compared with premenopausal women, the development of insulin resistance (IR) and non-alcoholic fatty liver disease (NAFLD) in obesity are more prevalent, where oestradiol (E2), the most potent female sex steroid, is proposed as the main culprit. Exogenous oestradiol (E2) administration exerts beneficial effects on antioxidant properties, restores total plasma antioxidant capacity and decreases biomarkers of OxS in ovariectomized animal models. Thus, we hypothesized that E2 treatment in states of obesity could have beneficial effects against OxS in the obese male's liver. We assumed that E2 could directly affect the level of OxS by increasing the level/activity of the AOS enzymes, particularly SOD1, SOD2, GPx, and CAT, in obese males' livers. In addition, we assumed that the level of malondialdehyde (MDA) and protein carbonyl content (PCC) in obese males' livers would be reduced after E2 treatment as a result of E2 inhibitory effect on lipid peroxidation and protein oxidation, respectively. To test our hypothesis, we used the liver of a high-fat (HF) diet-fed male Wistar rats as an animal model of obesity, treated with E2 intraperitoneally (40 μg/kg). Preliminary results from this study support our hypothesis that E2 increases liver protein expression of AOS enzymes: SOD1, GPx, and CAT, in control and HF male rats compared with their respective controls. The protein level of SOD2 and CAT activity was increased in HF treated with E2 compared with non-treated HF rats. Moreover, as we expected, E2 administration significantly decreased the MDA level in both E2-treated groups compared to their controls, while the PCC level was significantly decreased in HF treated group compared with untreated HF rats. In conclusion, the preliminary results we obtained in this study indicate that E2 administration can effectively inhibit the OxS-related processes in the liver in HF diet-induced obesity by increasing AOS enzymes levels and CAT activity, and also by decreasing levels of MDA and PCC. A consequence of our hypothesis is that treatment with E2 may be an innovative way to improve obesity-related liver disease prevention and healing. © 2023 Elsevier Ltd",
journal = "Medical Hypotheses",
title = "Does oestradiol treatment alleviate obesity-induced oxidative stress in the male liver?",
volume = "174",
pages = "111049",
doi = "10.1016/j.mehy.2023.111049"
}

Panić, A., Sudar-Milovanović, E., Stanimirović, J., Obradović, M. M., Zafirović, S., Soskić, S. S.,& Isenović, E. R.. (2023). Does oestradiol treatment alleviate obesity-induced oxidative stress in the male liver?. in Medical Hypotheses, 174, 111049.
https://doi.org/10.1016/j.mehy.2023.111049

Panić A, Sudar-Milovanović E, Stanimirović J, Obradović MM, Zafirović S, Soskić SS, Isenović ER. Does oestradiol treatment alleviate obesity-induced oxidative stress in the male liver?. in Medical Hypotheses. 2023;174:111049.
doi:10.1016/j.mehy.2023.111049 .

Panić, Anastasija, Sudar-Milovanović, Emina, Stanimirović, Julijana, Obradović, Milan M., Zafirović, Sonja, Soskić, Sanja S., Isenović, Esma R., "Does oestradiol treatment alleviate obesity-induced oxidative stress in the male liver?" in Medical Hypotheses, 174 (2023):111049,
https://doi.org/10.1016/j.mehy.2023.111049 . .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Sudar-Milovanović, Emina
AU  - Gluvić, Zoran
AU  - Banjac, Katarina
AU  - Rizzo, Manfredi
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10756
AB  - Cardiometabolic diseases (CMD) are a direct consequence of modern living and contribute to the development of multisystem diseases such as cardiovascular diseases and diabetes mellitus (DM). CMD has reached epidemic proportions worldwide. A sodium pump (Na + /K + -ATPase) is found in most eukaryotic cells’ membrane and controls many essential cellular functions directly or indirectly. This ion transporter and its isoforms are important in the pathogenesis of some pathological processes, including CMD. The structure and function of Na + /K + -ATPase, its expression and distribution in tissues, and its interactions with known ligands such as cardiotonic steroids and other suspected endogenous regulators are discussed in this review. In addition, we reviewed recent literature data related to the involvement of Na + /K + -ATPase activity dysfunction in CMD, focusing on the Na + /K + -ATPase as a potential therapeutic target in CMD.
T2  - Frontiers in Endocrinology
T1  - The Na+/K+-ATPase: A potential therapeutic target in cardiometabolic diseases
VL  - 14
DO  - 10.3389/fendo.2023.1150171
ER  - 

@article{
author = "Obradović, Milan M. and Sudar-Milovanović, Emina and Gluvić, Zoran and Banjac, Katarina and Rizzo, Manfredi and Isenović, Esma R.",
year = "2023",
abstract = "Cardiometabolic diseases (CMD) are a direct consequence of modern living and contribute to the development of multisystem diseases such as cardiovascular diseases and diabetes mellitus (DM). CMD has reached epidemic proportions worldwide. A sodium pump (Na + /K + -ATPase) is found in most eukaryotic cells’ membrane and controls many essential cellular functions directly or indirectly. This ion transporter and its isoforms are important in the pathogenesis of some pathological processes, including CMD. The structure and function of Na + /K + -ATPase, its expression and distribution in tissues, and its interactions with known ligands such as cardiotonic steroids and other suspected endogenous regulators are discussed in this review. In addition, we reviewed recent literature data related to the involvement of Na + /K + -ATPase activity dysfunction in CMD, focusing on the Na + /K + -ATPase as a potential therapeutic target in CMD.",
journal = "Frontiers in Endocrinology",
title = "The Na+/K+-ATPase: A potential therapeutic target in cardiometabolic diseases",
volume = "14",
doi = "10.3389/fendo.2023.1150171"
}

Obradović, M. M., Sudar-Milovanović, E., Gluvić, Z., Banjac, K., Rizzo, M.,& Isenović, E. R.. (2023). The Na+/K+-ATPase: A potential therapeutic target in cardiometabolic diseases. in Frontiers in Endocrinology, 14.
https://doi.org/10.3389/fendo.2023.1150171

Obradović MM, Sudar-Milovanović E, Gluvić Z, Banjac K, Rizzo M, Isenović ER. The Na+/K+-ATPase: A potential therapeutic target in cardiometabolic diseases. in Frontiers in Endocrinology. 2023;14.
doi:10.3389/fendo.2023.1150171 .

Obradović, Milan M., Sudar-Milovanović, Emina, Gluvić, Zoran, Banjac, Katarina, Rizzo, Manfredi, Isenović, Esma R., "The Na+/K+-ATPase: A potential therapeutic target in cardiometabolic diseases" in Frontiers in Endocrinology, 14 (2023),
https://doi.org/10.3389/fendo.2023.1150171 . .

TY  - JOUR
AU  - Mačvanin, Mirjana
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Stanimirović, Julijana
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10757
AB  - Metabolic diseases such as obesity, diabetes, dyslipidemia, and insulin resistance are characterized by glucose and lipid metabolism alterations and represent a global health problem. Many studies have established the crucial role of micro-ribonucleic acids (miRNAs) in controlling metabolic processes in various tissues. miRNAs are single-stranded, highly conserved non-coding RNAs containing 20-24 oligonucleotides that are expressed in a tissue-specific manner. miRNAs mainly interact through base pairing with 3' untranslated regions of target gene mRNAs to promote inhibition of their translation. miRNAs regulate the expression of as many as 30% of the human genes and have a role in crucial physiological processes such as human growth and development, cell proliferation, apoptosis, and metabolism. The number of miRNA molecules with a confirmed role in the pathogenesis of metabolic diseases is quickly expanding due to the availability of high-throughput methodologies for their identification. In this review, we present recent findings regarding the role of miRNAs as endocrine signaling molecules involved in the regulation of insulin production and fat metabolism. We discuss the potential of extracellular miRNAs present in biological fluids miRNAs as biomarkers for the prediction of diabetes and MetS. We also give an updated overview of therapeutic interventions based on antisense oligonucleotides and the CRISPR/Cas9 editing platform for manipulating levels of miRNAs involved in metabolic disorders. © 2023 Bentham Science Publishers.
T2  - Current Medicinal Chemistry
T1  - The Role of miRNAs in Metabolic Diseases
VL  - 30
IS  - 17
SP  - 1922
EP  - 1944
DO  - 10.2174/0929867329666220801161536
ER  - 

@article{
author = "Mačvanin, Mirjana and Obradović, Milan M. and Zafirović, Sonja and Stanimirović, Julijana and Isenović, Esma R.",
year = "2023",
abstract = "Metabolic diseases such as obesity, diabetes, dyslipidemia, and insulin resistance are characterized by glucose and lipid metabolism alterations and represent a global health problem. Many studies have established the crucial role of micro-ribonucleic acids (miRNAs) in controlling metabolic processes in various tissues. miRNAs are single-stranded, highly conserved non-coding RNAs containing 20-24 oligonucleotides that are expressed in a tissue-specific manner. miRNAs mainly interact through base pairing with 3' untranslated regions of target gene mRNAs to promote inhibition of their translation. miRNAs regulate the expression of as many as 30% of the human genes and have a role in crucial physiological processes such as human growth and development, cell proliferation, apoptosis, and metabolism. The number of miRNA molecules with a confirmed role in the pathogenesis of metabolic diseases is quickly expanding due to the availability of high-throughput methodologies for their identification. In this review, we present recent findings regarding the role of miRNAs as endocrine signaling molecules involved in the regulation of insulin production and fat metabolism. We discuss the potential of extracellular miRNAs present in biological fluids miRNAs as biomarkers for the prediction of diabetes and MetS. We also give an updated overview of therapeutic interventions based on antisense oligonucleotides and the CRISPR/Cas9 editing platform for manipulating levels of miRNAs involved in metabolic disorders. © 2023 Bentham Science Publishers.",
journal = "Current Medicinal Chemistry",
title = "The Role of miRNAs in Metabolic Diseases",
volume = "30",
number = "17",
pages = "1922-1944",
doi = "10.2174/0929867329666220801161536"
}

Mačvanin, M., Obradović, M. M., Zafirović, S., Stanimirović, J.,& Isenović, E. R.. (2023). The Role of miRNAs in Metabolic Diseases. in Current Medicinal Chemistry, 30(17), 1922-1944.
https://doi.org/10.2174/0929867329666220801161536

Mačvanin M, Obradović MM, Zafirović S, Stanimirović J, Isenović ER. The Role of miRNAs in Metabolic Diseases. in Current Medicinal Chemistry. 2023;30(17):1922-1944.
doi:10.2174/0929867329666220801161536 .

Mačvanin, Mirjana, Obradović, Milan M., Zafirović, Sonja, Stanimirović, Julijana, Isenović, Esma R., "The Role of miRNAs in Metabolic Diseases" in Current Medicinal Chemistry, 30, no. 17 (2023):1922-1944,
https://doi.org/10.2174/0929867329666220801161536 . .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Samardžić, Vladimir
AU  - Mačvanin, Mirjana
AU  - Zafirović, Sonja
AU  - Gluvić, Zoran
AU  - Grubin, Jasmina
AU  - Gao, Xin
AU  - Essack, Magbubah
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11567
AB  - Thyroid nodules (TN) are localized morphological changes in the thyroid gland and can be benign or malignant.Objective: The present study investigates the relationships between biochemical markers in serum (s) and their homologs in washout (w) after fine-needle aspiration biopsy (FNAB) of the TN of interest and their correlation with cytology specimen findings.We investigated the relationships between serum biochemical markers nitric oxide (NO), thyroglobulin (TG), and calcitonin (CT), their homologs in washout after FNAB of the TN of interest, and cytology findings of biopsy samples classified according to the Bethesda system for thyroid cytopathology in this study, which included 86 subjects.Results: Washout TG (TGw) level positively correlates with the cytology finding of the biopsy. A higher level of TGw correlates with higher categories of the Bethesda classification and indicates a higher malignant potential. The levels of serum NO (NOs), serum TG (TGs), serum CT (CTs), and washout CT (CTw) do not correlate with the cytology finding of the biopsy, and the higher levels of washout NO (NOw) correspond to the more suspicious ultrasound findings.The findings of our study suggest that TGw and NOw could be used as potential predictors of malignancy in TN.
T2  - Frontiers in Endocrinology
T1  - Nitric oxide, thyroglobulin, and calcitonin: Unravelling the nature of thyroid nodules
VL  - 14
SP  - 1241223
DO  - 10.3389/fendo.2023.1241223
ER  - 

@article{
author = "Obradović, Milan M. and Samardžić, Vladimir and Mačvanin, Mirjana and Zafirović, Sonja and Gluvić, Zoran and Grubin, Jasmina and Gao, Xin and Essack, Magbubah and Isenović, Esma R.",
year = "2023",
abstract = "Thyroid nodules (TN) are localized morphological changes in the thyroid gland and can be benign or malignant.Objective: The present study investigates the relationships between biochemical markers in serum (s) and their homologs in washout (w) after fine-needle aspiration biopsy (FNAB) of the TN of interest and their correlation with cytology specimen findings.We investigated the relationships between serum biochemical markers nitric oxide (NO), thyroglobulin (TG), and calcitonin (CT), their homologs in washout after FNAB of the TN of interest, and cytology findings of biopsy samples classified according to the Bethesda system for thyroid cytopathology in this study, which included 86 subjects.Results: Washout TG (TGw) level positively correlates with the cytology finding of the biopsy. A higher level of TGw correlates with higher categories of the Bethesda classification and indicates a higher malignant potential. The levels of serum NO (NOs), serum TG (TGs), serum CT (CTs), and washout CT (CTw) do not correlate with the cytology finding of the biopsy, and the higher levels of washout NO (NOw) correspond to the more suspicious ultrasound findings.The findings of our study suggest that TGw and NOw could be used as potential predictors of malignancy in TN.",
journal = "Frontiers in Endocrinology",
title = "Nitric oxide, thyroglobulin, and calcitonin: Unravelling the nature of thyroid nodules",
volume = "14",
pages = "1241223",
doi = "10.3389/fendo.2023.1241223"
}

Obradović, M. M., Samardžić, V., Mačvanin, M., Zafirović, S., Gluvić, Z., Grubin, J., Gao, X., Essack, M.,& Isenović, E. R.. (2023). Nitric oxide, thyroglobulin, and calcitonin: Unravelling the nature of thyroid nodules. in Frontiers in Endocrinology, 14, 1241223.
https://doi.org/10.3389/fendo.2023.1241223

Obradović MM, Samardžić V, Mačvanin M, Zafirović S, Gluvić Z, Grubin J, Gao X, Essack M, Isenović ER. Nitric oxide, thyroglobulin, and calcitonin: Unravelling the nature of thyroid nodules. in Frontiers in Endocrinology. 2023;14:1241223.
doi:10.3389/fendo.2023.1241223 .

Obradović, Milan M., Samardžić, Vladimir, Mačvanin, Mirjana, Zafirović, Sonja, Gluvić, Zoran, Grubin, Jasmina, Gao, Xin, Essack, Magbubah, Isenović, Esma R., "Nitric oxide, thyroglobulin, and calcitonin: Unravelling the nature of thyroid nodules" in Frontiers in Endocrinology, 14 (2023):1241223,
https://doi.org/10.3389/fendo.2023.1241223 . .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Gluvić, Zoran
AU  - Radovanović, Jelena
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11639
AB  - The current literature findings on autophagy’s beneficial and detrimental roles in diabetes mellitus (DM) and diabetes-related comorbidities were reviewed. The effects of oral hypoglycaemic medicines and autophagy in DM. Autophagy plays an important function in cellular homeostasis by promoting cell survival or initiating cell death in physiological settings was also assessed. Although autophagy protects insulin-target tissues, organelle failure caused by autophagy malfunction influences DM and other metabolic diseases. Endoplasmic reticulum and oxidative stress enhance autophagy levels, making it easier to regulate stress-induced intracellular changes. Evidence suggests that autophagy-caused cell death can occur when autophagy is overstimulated and constitutively activated, which might prevent or develop DM. Even though the precise role of autophagy in DM complications is uncertain, deregulation of the autophagic machinery is strongly linked to beta cell destruction and the aetiology of DM. Thus, improving autophagy dysfunction is a possible therapeutic objective in treating DM and other metabolic disorders.
T2  - Exploration of Medicine
T1  - Autophagy and diabetes
SP  - 576
EP  - 588
DO  - 10.37349/emed.2023.00162
ER  - 

@article{
author = "Obradović, Milan M. and Zafirović, Sonja and Gluvić, Zoran and Radovanović, Jelena and Isenović, Esma R.",
year = "2023",
abstract = "The current literature findings on autophagy’s beneficial and detrimental roles in diabetes mellitus (DM) and diabetes-related comorbidities were reviewed. The effects of oral hypoglycaemic medicines and autophagy in DM. Autophagy plays an important function in cellular homeostasis by promoting cell survival or initiating cell death in physiological settings was also assessed. Although autophagy protects insulin-target tissues, organelle failure caused by autophagy malfunction influences DM and other metabolic diseases. Endoplasmic reticulum and oxidative stress enhance autophagy levels, making it easier to regulate stress-induced intracellular changes. Evidence suggests that autophagy-caused cell death can occur when autophagy is overstimulated and constitutively activated, which might prevent or develop DM. Even though the precise role of autophagy in DM complications is uncertain, deregulation of the autophagic machinery is strongly linked to beta cell destruction and the aetiology of DM. Thus, improving autophagy dysfunction is a possible therapeutic objective in treating DM and other metabolic disorders.",
journal = "Exploration of Medicine",
title = "Autophagy and diabetes",
pages = "576-588",
doi = "10.37349/emed.2023.00162"
}

Obradović, M. M., Zafirović, S., Gluvić, Z., Radovanović, J.,& Isenović, E. R.. (2023). Autophagy and diabetes. in Exploration of Medicine, 576-588.
https://doi.org/10.37349/emed.2023.00162

Obradović MM, Zafirović S, Gluvić Z, Radovanović J, Isenović ER. Autophagy and diabetes. in Exploration of Medicine. 2023;:576-588.
doi:10.37349/emed.2023.00162 .

Obradović, Milan M., Zafirović, Sonja, Gluvić, Zoran, Radovanović, Jelena, Isenović, Esma R., "Autophagy and diabetes" in Exploration of Medicine (2023):576-588,
https://doi.org/10.37349/emed.2023.00162 . .

TY  - JOUR
AU  - Mačvanin, Mirjana
AU  - Gluvić, Zoran
AU  - Radovanović, Jelena
AU  - Essack, Magbubah
AU  - Gao, Xin
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10686
AB  - Cardiovascular (CV) disorders are steadily increasing, making them the world’s most prevalent health issue. New research highlights the importance of insulin-like growth factor 1 (IGF-1) for maintaining CV healthMethodsWe searched PubMed and MEDLINE for English and non-English articles with English abstracts published between 1957 (when the first report on IGF-1 identification was published) and 2022. The top search terms were: IGF-1, cardiovascular disease, IGF-1 receptors, IGF-1 and microRNAs, therapeutic interventions with IGF-1, IGF-1 and diabetes, IGF-1 and cardiovascular disease. The search retrieved original peer-reviewed articles, which were further analyzed, focusing on the role of IGF-1 in pathophysiological conditions. We specifically focused on including the most recent findings published in the past five years.ResultsIGF-1, an anabolic growth factor, regulates cell division, proliferation, and survival. In addition to its well-known growth-promoting and metabolic effects, there is mounting evidence that IGF-1 plays a specialized role in the complex activities that underpin CV function. IGF-1 promotes cardiac development and improves cardiac output, stroke volume, contractility, and ejection fraction. Furthermore, IGF-1 mediates many growth hormones (GH) actions. IGF-1 stimulates contractility and tissue remodeling in humans to improve heart function after myocardial infarction. IGF-1 also improves the lipid profile, lowers insulin levels, increases insulin sensitivity, and promotes glucose metabolism. These findings point to the intriguing medicinal potential of IGF-1. Human studies associate low serum levels of free or total IGF-1 with an increased risk of CV and cerebrovascular illness. Extensive human trials are being conducted to investigate the therapeutic efficacy and outcomes of IGF-1-related therapy.DiscussionWe anticipate the development of novel IGF-1-related therapy with minimal side effects. This review discusses recent findings on the role of IGF-1 in the cardiovascular (CVD) system, including both normal and pathological conditions. We also discuss progress in therapeutic interventions aimed at targeting the IGF axis and provide insights into the epigenetic regulation of IGF-1 mediated by microRNAs.
T2  - Frontiers in Endocrinology
T1  - New insights on the cardiovascular effects of IGF-1
VL  - 14
SP  - 1142644
DO  - 10.3389/fendo.2023.1142644
ER  - 

@article{
author = "Mačvanin, Mirjana and Gluvić, Zoran and Radovanović, Jelena and Essack, Magbubah and Gao, Xin and Isenović, Esma R.",
year = "2023",
abstract = "Cardiovascular (CV) disorders are steadily increasing, making them the world’s most prevalent health issue. New research highlights the importance of insulin-like growth factor 1 (IGF-1) for maintaining CV healthMethodsWe searched PubMed and MEDLINE for English and non-English articles with English abstracts published between 1957 (when the first report on IGF-1 identification was published) and 2022. The top search terms were: IGF-1, cardiovascular disease, IGF-1 receptors, IGF-1 and microRNAs, therapeutic interventions with IGF-1, IGF-1 and diabetes, IGF-1 and cardiovascular disease. The search retrieved original peer-reviewed articles, which were further analyzed, focusing on the role of IGF-1 in pathophysiological conditions. We specifically focused on including the most recent findings published in the past five years.ResultsIGF-1, an anabolic growth factor, regulates cell division, proliferation, and survival. In addition to its well-known growth-promoting and metabolic effects, there is mounting evidence that IGF-1 plays a specialized role in the complex activities that underpin CV function. IGF-1 promotes cardiac development and improves cardiac output, stroke volume, contractility, and ejection fraction. Furthermore, IGF-1 mediates many growth hormones (GH) actions. IGF-1 stimulates contractility and tissue remodeling in humans to improve heart function after myocardial infarction. IGF-1 also improves the lipid profile, lowers insulin levels, increases insulin sensitivity, and promotes glucose metabolism. These findings point to the intriguing medicinal potential of IGF-1. Human studies associate low serum levels of free or total IGF-1 with an increased risk of CV and cerebrovascular illness. Extensive human trials are being conducted to investigate the therapeutic efficacy and outcomes of IGF-1-related therapy.DiscussionWe anticipate the development of novel IGF-1-related therapy with minimal side effects. This review discusses recent findings on the role of IGF-1 in the cardiovascular (CVD) system, including both normal and pathological conditions. We also discuss progress in therapeutic interventions aimed at targeting the IGF axis and provide insights into the epigenetic regulation of IGF-1 mediated by microRNAs.",
journal = "Frontiers in Endocrinology",
title = "New insights on the cardiovascular effects of IGF-1",
volume = "14",
pages = "1142644",
doi = "10.3389/fendo.2023.1142644"
}

Mačvanin, M., Gluvić, Z., Radovanović, J., Essack, M., Gao, X.,& Isenović, E. R.. (2023). New insights on the cardiovascular effects of IGF-1. in Frontiers in Endocrinology, 14, 1142644.
https://doi.org/10.3389/fendo.2023.1142644

Mačvanin M, Gluvić Z, Radovanović J, Essack M, Gao X, Isenović ER. New insights on the cardiovascular effects of IGF-1. in Frontiers in Endocrinology. 2023;14:1142644.
doi:10.3389/fendo.2023.1142644 .

Mačvanin, Mirjana, Gluvić, Zoran, Radovanović, Jelena, Essack, Magbubah, Gao, Xin, Isenović, Esma R., "New insights on the cardiovascular effects of IGF-1" in Frontiers in Endocrinology, 14 (2023):1142644,
https://doi.org/10.3389/fendo.2023.1142644 . .

TY  - JOUR
AU  - Mačvanin, Mirjana
AU  - Gluvić, Zoran
AU  - Radovanović, Jelena
AU  - Essack, Magbubah
AU  - Gao, Xin
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10774
AB  - Diabetes mellitus (DM) is on the rise, necessitating the development of novel therapeutic and preventive strategies to mitigate the disease’s debilitating effects. Diabetic cardiomyopathy (DCMP) is among the leading causes of morbidity and mortality in diabetic patients globally. DCMP manifests as cardiomyocyte hypertrophy, apoptosis, and myocardial interstitial fibrosis before progressing to heart failure. Evidence suggests that non-coding RNAs, such as long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), regulate diabetic cardiomyopathy-related processes such as insulin resistance, cardiomyocyte apoptosis and inflammation, emphasizing their heart-protective effects. This paper reviewed the literature data from animal and human studies on the non-trivial roles of miRNAs and lncRNAs in the context of DCMP in diabetes and demonstrated their future potential in DCMP treatment in diabetic patients.
T2  - Frontiers in Endocrinology
T1  - Diabetic cardiomyopathy: The role of microRNAs and long non-coding RNAs
VL  - 14
DO  - 10.3389/fendo.2023.1124613
ER  - 

@article{
author = "Mačvanin, Mirjana and Gluvić, Zoran and Radovanović, Jelena and Essack, Magbubah and Gao, Xin and Isenović, Esma R.",
year = "2023",
abstract = "Diabetes mellitus (DM) is on the rise, necessitating the development of novel therapeutic and preventive strategies to mitigate the disease’s debilitating effects. Diabetic cardiomyopathy (DCMP) is among the leading causes of morbidity and mortality in diabetic patients globally. DCMP manifests as cardiomyocyte hypertrophy, apoptosis, and myocardial interstitial fibrosis before progressing to heart failure. Evidence suggests that non-coding RNAs, such as long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), regulate diabetic cardiomyopathy-related processes such as insulin resistance, cardiomyocyte apoptosis and inflammation, emphasizing their heart-protective effects. This paper reviewed the literature data from animal and human studies on the non-trivial roles of miRNAs and lncRNAs in the context of DCMP in diabetes and demonstrated their future potential in DCMP treatment in diabetic patients.",
journal = "Frontiers in Endocrinology",
title = "Diabetic cardiomyopathy: The role of microRNAs and long non-coding RNAs",
volume = "14",
doi = "10.3389/fendo.2023.1124613"
}

Mačvanin, M., Gluvić, Z., Radovanović, J., Essack, M., Gao, X.,& Isenović, E. R.. (2023). Diabetic cardiomyopathy: The role of microRNAs and long non-coding RNAs. in Frontiers in Endocrinology, 14.
https://doi.org/10.3389/fendo.2023.1124613

Mačvanin M, Gluvić Z, Radovanović J, Essack M, Gao X, Isenović ER. Diabetic cardiomyopathy: The role of microRNAs and long non-coding RNAs. in Frontiers in Endocrinology. 2023;14.
doi:10.3389/fendo.2023.1124613 .

Mačvanin, Mirjana, Gluvić, Zoran, Radovanović, Jelena, Essack, Magbubah, Gao, Xin, Isenović, Esma R., "Diabetic cardiomyopathy: The role of microRNAs and long non-coding RNAs" in Frontiers in Endocrinology, 14 (2023),
https://doi.org/10.3389/fendo.2023.1124613 . .

TY  - JOUR
AU  - Mačvanin, Mirjana
AU  - Gluvić, Zoran
AU  - Zafirović, Sonja
AU  - Gao, Xin
AU  - Essack, Magbubah
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10613
AB  - An imbalance between pro-oxidative and antioxidative cellular mechanisms is oxidative stress (OxS) which may be systemic or organ-specific. Although OxS is a consequence of normal body and organ physiology, severely impaired oxidative homeostasis results in DNA hydroxylation, protein denaturation, lipid peroxidation, and apoptosis, ultimately compromising cells’ function and viability. The thyroid gland is an organ that exhibits both oxidative and antioxidative processes. In terms of OxS severity, the thyroid gland’s response could be physiological (i.e. hormone production and secretion) or pathological (i.e. development of diseases, such as goitre, thyroid cancer, or thyroiditis). Protective nutritional antioxidants may benefit defensive antioxidative systems in resolving pro-oxidative dominance and redox imbalance, preventing or delaying chronic thyroid diseases. This review provides information on nutritional antioxidants and their protective roles against impaired redox homeostasis in various thyroid pathologies. We also review novel findings related to the connection between the thyroid gland and gut microbiome and analyze the effects of probiotics with antioxidant properties on thyroid diseases. Copyright © 2023 Macvanin, Gluvic, Zafirovic, Gao, Essack and Isenovic.
T2  - Frontiers in Endocrinology
T1  - The protective role of nutritional antioxidants against oxidative stress in thyroid disorders
VL  - 13
DO  - 10.3389/fendo.2022.1092837
ER  - 

@article{
author = "Mačvanin, Mirjana and Gluvić, Zoran and Zafirović, Sonja and Gao, Xin and Essack, Magbubah and Isenović, Esma R.",
year = "2023",
abstract = "An imbalance between pro-oxidative and antioxidative cellular mechanisms is oxidative stress (OxS) which may be systemic or organ-specific. Although OxS is a consequence of normal body and organ physiology, severely impaired oxidative homeostasis results in DNA hydroxylation, protein denaturation, lipid peroxidation, and apoptosis, ultimately compromising cells’ function and viability. The thyroid gland is an organ that exhibits both oxidative and antioxidative processes. In terms of OxS severity, the thyroid gland’s response could be physiological (i.e. hormone production and secretion) or pathological (i.e. development of diseases, such as goitre, thyroid cancer, or thyroiditis). Protective nutritional antioxidants may benefit defensive antioxidative systems in resolving pro-oxidative dominance and redox imbalance, preventing or delaying chronic thyroid diseases. This review provides information on nutritional antioxidants and their protective roles against impaired redox homeostasis in various thyroid pathologies. We also review novel findings related to the connection between the thyroid gland and gut microbiome and analyze the effects of probiotics with antioxidant properties on thyroid diseases. Copyright © 2023 Macvanin, Gluvic, Zafirovic, Gao, Essack and Isenovic.",
journal = "Frontiers in Endocrinology",
title = "The protective role of nutritional antioxidants against oxidative stress in thyroid disorders",
volume = "13",
doi = "10.3389/fendo.2022.1092837"
}

Mačvanin, M., Gluvić, Z., Zafirović, S., Gao, X., Essack, M.,& Isenović, E. R.. (2023). The protective role of nutritional antioxidants against oxidative stress in thyroid disorders. in Frontiers in Endocrinology, 13.
https://doi.org/10.3389/fendo.2022.1092837

Mačvanin M, Gluvić Z, Zafirović S, Gao X, Essack M, Isenović ER. The protective role of nutritional antioxidants against oxidative stress in thyroid disorders. in Frontiers in Endocrinology. 2023;13.
doi:10.3389/fendo.2022.1092837 .

Mačvanin, Mirjana, Gluvić, Zoran, Zafirović, Sonja, Gao, Xin, Essack, Magbubah, Isenović, Esma R., "The protective role of nutritional antioxidants against oxidative stress in thyroid disorders" in Frontiers in Endocrinology, 13 (2023),
https://doi.org/10.3389/fendo.2022.1092837 . .

TY  - JOUR
AU  - Mačvanin, Mirjana
AU  - Gluvić, Zoran
AU  - Bajić, Vladan
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11574
AB  - Diabetes mellitus (DM) is a group of metabolic disorders defined by hyperglycemia induced by insulin resistance, inadequate insulin secretion, or excessive glucagon secretion. In 2021, the global prevalence of diabetes is anticipated to be 10.7% (537 million people). Noncoding RNAs (ncRNAs) appear to have an important role in the initiation and progression of DM, according to a growing body of research. The two major groups of ncRNAs implicated in diabetic disorders are miRNAs and long noncoding RNAs. miRNAs are singlestranded, short (17–25 nucleotides), ncRNAs that influence gene expression at the post-transcriptional level. Because DM has reached epidemic proportions worldwide, it appears that novel diagnostic and therapeutic strategies are required to identify and treat complications associated with these diseases efficiently. miRNAs are gaining attention as biomarkers for DM diagnosis and potential treatment due to their function in maintaining physiological homeostasis via gene expression regulation. In this review, we address the issue of the gradually expanding global prevalence of DM by presenting a complete and upto-date synopsis of various regulatory miRNAs involved in these disorders. We hope this review will spark discussion about ncRNAs as prognostic biomarkers and therapeutic tools for DM. We examine and synthesize recent research that used novel, high-throughput technologies to uncover ncRNAs involved in DM, necessitating a systematic approach to examining and summarizing their roles and possible diagnostic and therapeutic uses.
T2  - World Journal of Diabetes
T1  - Novel insights regarding the role of noncoding RNAs in diabetes
VL  - 14
IS  - 7
SP  - 958
EP  - 976
DO  - 10.4239/wjd.v14.i7.958
ER  - 

@article{
author = "Mačvanin, Mirjana and Gluvić, Zoran and Bajić, Vladan and Isenović, Esma R.",
year = "2023",
abstract = "Diabetes mellitus (DM) is a group of metabolic disorders defined by hyperglycemia induced by insulin resistance, inadequate insulin secretion, or excessive glucagon secretion. In 2021, the global prevalence of diabetes is anticipated to be 10.7% (537 million people). Noncoding RNAs (ncRNAs) appear to have an important role in the initiation and progression of DM, according to a growing body of research. The two major groups of ncRNAs implicated in diabetic disorders are miRNAs and long noncoding RNAs. miRNAs are singlestranded, short (17–25 nucleotides), ncRNAs that influence gene expression at the post-transcriptional level. Because DM has reached epidemic proportions worldwide, it appears that novel diagnostic and therapeutic strategies are required to identify and treat complications associated with these diseases efficiently. miRNAs are gaining attention as biomarkers for DM diagnosis and potential treatment due to their function in maintaining physiological homeostasis via gene expression regulation. In this review, we address the issue of the gradually expanding global prevalence of DM by presenting a complete and upto-date synopsis of various regulatory miRNAs involved in these disorders. We hope this review will spark discussion about ncRNAs as prognostic biomarkers and therapeutic tools for DM. We examine and synthesize recent research that used novel, high-throughput technologies to uncover ncRNAs involved in DM, necessitating a systematic approach to examining and summarizing their roles and possible diagnostic and therapeutic uses.",
journal = "World Journal of Diabetes",
title = "Novel insights regarding the role of noncoding RNAs in diabetes",
volume = "14",
number = "7",
pages = "958-976",
doi = "10.4239/wjd.v14.i7.958"
}

Mačvanin, M., Gluvić, Z., Bajić, V.,& Isenović, E. R.. (2023). Novel insights regarding the role of noncoding RNAs in diabetes. in World Journal of Diabetes, 14(7), 958-976.
https://doi.org/10.4239/wjd.v14.i7.958

Mačvanin M, Gluvić Z, Bajić V, Isenović ER. Novel insights regarding the role of noncoding RNAs in diabetes. in World Journal of Diabetes. 2023;14(7):958-976.
doi:10.4239/wjd.v14.i7.958 .

Mačvanin, Mirjana, Gluvić, Zoran, Bajić, Vladan, Isenović, Esma R., "Novel insights regarding the role of noncoding RNAs in diabetes" in World Journal of Diabetes, 14, no. 7 (2023):958-976,
https://doi.org/10.4239/wjd.v14.i7.958 . .

TY  - JOUR
AU  - Al-Maini, Mustafa
AU  - Maindarkar, Mahesh
AU  - Kitas, George D.
AU  - Khanna, Narendra N.
AU  - Misra, Durga Prasanna
AU  - Johri, Amer M.
AU  - Mantella, Laura
AU  - Agarwal, Vikas
AU  - Sharma, Aman
AU  - Singh, Inder M.
AU  - Tsoulfas, George
AU  - Laird, John R.
AU  - Faa, Gavino
AU  - Teji, Jagjit
AU  - Turk, Monika
AU  - Visković, Klaudija
AU  - Ruzsa, Zoltan
AU  - Mavrogeni, Sophie
AU  - Rathore, Vijay
AU  - Miner, Martin
AU  - Kalra, Manudeep K.
AU  - Isenović, Esma R.
AU  - Saba, Luca
AU  - Fouda, Mostafa M.
AU  - Suri, Jasjit S.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12947
AB  - The challenges associated with diagnosing and treating cardiovascular disease (CVD)/Stroke in Rheumatoid arthritis (RA) arise from the delayed onset of symptoms. Existing clinical risk scores are inadequate in predicting cardiac events, and conventional risk factors alone do not accurately classify many individuals at risk. Several CVD biomarkers consider the multiple pathways involved in the development of atherosclerosis, which is the primary cause of CVD/Stroke in RA. To enhance the accuracy of CVD/Stroke risk assessment in the RA framework, a proposed approach involves combining genomic-based biomarkers (GBBM) derived from plasma and/or serum samples with innovative non-invasive radiomic-based biomarkers (RBBM), such as measurements of synovial fluid, plaque area, and plaque burden. This review presents two hypotheses: (i) RBBM and GBBM biomarkers exhibit a significant correlation and can precisely detect the severity of CVD/Stroke in RA patients. (ii) Artificial Intelligence (AI)-based preventive, precision, and personalized (aiP3) CVD/Stroke risk AtheroEdge™ model (AtheroPoint™, CA, USA) that utilizes deep learning (DL) to accurately classify the risk of CVD/stroke in RA framework. The authors conducted a comprehensive search using the PRISMA technique, identifying 153 studies that assessed the features/biomarkers of RBBM and GBBM for CVD/Stroke. The study demonstrates how DL models can be integrated into the AtheroEdge™–aiP3 framework to determine the risk of CVD/Stroke in RA patients. The findings of this review suggest that the combination of RBBM with GBBM introduces a new dimension to the assessment of CVD/Stroke risk in the RA framework. Synovial fluid levels that are higher than normal lead to an increase in the plaque burden. Additionally, the review provides recommendations for novel, unbiased, and pruned DL algorithms that can predict CVD/Stroke risk within a RA framework that is preventive, precise, and personalized.
T2  - Rheumatology International
T1  - Artificial intelligence-based preventive, personalized and precision medicine for cardiovascular disease/stroke risk assessment in rheumatoid arthritis patients: a narrative review
VL  - 43
IS  - 11
SP  - 1965
EP  - 1982
DO  - 10.1007/s00296-023-05415-1
ER  - 

@article{
author = "Al-Maini, Mustafa and Maindarkar, Mahesh and Kitas, George D. and Khanna, Narendra N. and Misra, Durga Prasanna and Johri, Amer M. and Mantella, Laura and Agarwal, Vikas and Sharma, Aman and Singh, Inder M. and Tsoulfas, George and Laird, John R. and Faa, Gavino and Teji, Jagjit and Turk, Monika and Visković, Klaudija and Ruzsa, Zoltan and Mavrogeni, Sophie and Rathore, Vijay and Miner, Martin and Kalra, Manudeep K. and Isenović, Esma R. and Saba, Luca and Fouda, Mostafa M. and Suri, Jasjit S.",
year = "2023",
abstract = "The challenges associated with diagnosing and treating cardiovascular disease (CVD)/Stroke in Rheumatoid arthritis (RA) arise from the delayed onset of symptoms. Existing clinical risk scores are inadequate in predicting cardiac events, and conventional risk factors alone do not accurately classify many individuals at risk. Several CVD biomarkers consider the multiple pathways involved in the development of atherosclerosis, which is the primary cause of CVD/Stroke in RA. To enhance the accuracy of CVD/Stroke risk assessment in the RA framework, a proposed approach involves combining genomic-based biomarkers (GBBM) derived from plasma and/or serum samples with innovative non-invasive radiomic-based biomarkers (RBBM), such as measurements of synovial fluid, plaque area, and plaque burden. This review presents two hypotheses: (i) RBBM and GBBM biomarkers exhibit a significant correlation and can precisely detect the severity of CVD/Stroke in RA patients. (ii) Artificial Intelligence (AI)-based preventive, precision, and personalized (aiP3) CVD/Stroke risk AtheroEdge™ model (AtheroPoint™, CA, USA) that utilizes deep learning (DL) to accurately classify the risk of CVD/stroke in RA framework. The authors conducted a comprehensive search using the PRISMA technique, identifying 153 studies that assessed the features/biomarkers of RBBM and GBBM for CVD/Stroke. The study demonstrates how DL models can be integrated into the AtheroEdge™–aiP3 framework to determine the risk of CVD/Stroke in RA patients. The findings of this review suggest that the combination of RBBM with GBBM introduces a new dimension to the assessment of CVD/Stroke risk in the RA framework. Synovial fluid levels that are higher than normal lead to an increase in the plaque burden. Additionally, the review provides recommendations for novel, unbiased, and pruned DL algorithms that can predict CVD/Stroke risk within a RA framework that is preventive, precise, and personalized.",
journal = "Rheumatology International",
title = "Artificial intelligence-based preventive, personalized and precision medicine for cardiovascular disease/stroke risk assessment in rheumatoid arthritis patients: a narrative review",
volume = "43",
number = "11",
pages = "1965-1982",
doi = "10.1007/s00296-023-05415-1"
}

Al-Maini, M., Maindarkar, M., Kitas, G. D., Khanna, N. N., Misra, D. P., Johri, A. M., Mantella, L., Agarwal, V., Sharma, A., Singh, I. M., Tsoulfas, G., Laird, J. R., Faa, G., Teji, J., Turk, M., Visković, K., Ruzsa, Z., Mavrogeni, S., Rathore, V., Miner, M., Kalra, M. K., Isenović, E. R., Saba, L., Fouda, M. M.,& Suri, J. S.. (2023). Artificial intelligence-based preventive, personalized and precision medicine for cardiovascular disease/stroke risk assessment in rheumatoid arthritis patients: a narrative review. in Rheumatology International, 43(11), 1965-1982.
https://doi.org/10.1007/s00296-023-05415-1

Al-Maini M, Maindarkar M, Kitas GD, Khanna NN, Misra DP, Johri AM, Mantella L, Agarwal V, Sharma A, Singh IM, Tsoulfas G, Laird JR, Faa G, Teji J, Turk M, Visković K, Ruzsa Z, Mavrogeni S, Rathore V, Miner M, Kalra MK, Isenović ER, Saba L, Fouda MM, Suri JS. Artificial intelligence-based preventive, personalized and precision medicine for cardiovascular disease/stroke risk assessment in rheumatoid arthritis patients: a narrative review. in Rheumatology International. 2023;43(11):1965-1982.
doi:10.1007/s00296-023-05415-1 .

Al-Maini, Mustafa, Maindarkar, Mahesh, Kitas, George D., Khanna, Narendra N., Misra, Durga Prasanna, Johri, Amer M., Mantella, Laura, Agarwal, Vikas, Sharma, Aman, Singh, Inder M., Tsoulfas, George, Laird, John R., Faa, Gavino, Teji, Jagjit, Turk, Monika, Visković, Klaudija, Ruzsa, Zoltan, Mavrogeni, Sophie, Rathore, Vijay, Miner, Martin, Kalra, Manudeep K., Isenović, Esma R., Saba, Luca, Fouda, Mostafa M., Suri, Jasjit S., "Artificial intelligence-based preventive, personalized and precision medicine for cardiovascular disease/stroke risk assessment in rheumatoid arthritis patients: a narrative review" in Rheumatology International, 43, no. 11 (2023):1965-1982,
https://doi.org/10.1007/s00296-023-05415-1 . .

TY  - JOUR
AU  - Tomasović, M.
AU  - Sinik, M.
AU  - Gluvić, Zoran
AU  - Zafirović, Sonja
AU  - Isenović, Esma
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12878
AB  - Context. Propylthiouracil (PTU) could cause lupus or vasculitis-like hypersensitivities thus interfering with some other concomitant diseases. Objective. Clinicians must be aware of the side effects of medications, particularly after their introduction and long-term use. Some clinical manifestations may be similar to well-known drug side effects or hypersensitivity. Every unusual clinical scenario related to drug use must be evaluated individually and thoroughly. Subjects and Methods. Hands and feet skin changes were observed several days after PTU administration in a male patient with severe diffuse toxic goitre. A complete blood count, biochemistry analyses, thyroid function tests and antibodies, and immunology analyses were performed. Results. As the skin changes were distributed regionally, liver function tests were normal, and there were no signs of clinical deterioration, it was decided to continue PTU treatment and monitor the patient. The initial maculopapular rash quickly turned vesicular, then scaly. After two weeks, the skin changes were wholly restored, with no scarring. Hand, Foot, and Mouth disease (HFMD) was diagnosed after a thorough epidemiological survey and clinical workout. Conclusions. Our case study demonstrates that skin changes associated with HFMD may resemble those associated with PTU-induced vasculitis.
T2  - Acta Endocrinologica (Bucharest)
T1  - Case Report of Hand and Foot Skin Changes Resembling PTU-Induced Vasculitis in a Young Male with Diffuse Toxic Goitre
VL  - 19
IS  - 3
SP  - 380
EP  - 385
DO  - 10.4183/aeb.2023.380
ER  - 

@article{
author = "Tomasović, M. and Sinik, M. and Gluvić, Zoran and Zafirović, Sonja and Isenović, Esma",
year = "2023",
abstract = "Context. Propylthiouracil (PTU) could cause lupus or vasculitis-like hypersensitivities thus interfering with some other concomitant diseases. Objective. Clinicians must be aware of the side effects of medications, particularly after their introduction and long-term use. Some clinical manifestations may be similar to well-known drug side effects or hypersensitivity. Every unusual clinical scenario related to drug use must be evaluated individually and thoroughly. Subjects and Methods. Hands and feet skin changes were observed several days after PTU administration in a male patient with severe diffuse toxic goitre. A complete blood count, biochemistry analyses, thyroid function tests and antibodies, and immunology analyses were performed. Results. As the skin changes were distributed regionally, liver function tests were normal, and there were no signs of clinical deterioration, it was decided to continue PTU treatment and monitor the patient. The initial maculopapular rash quickly turned vesicular, then scaly. After two weeks, the skin changes were wholly restored, with no scarring. Hand, Foot, and Mouth disease (HFMD) was diagnosed after a thorough epidemiological survey and clinical workout. Conclusions. Our case study demonstrates that skin changes associated with HFMD may resemble those associated with PTU-induced vasculitis.",
journal = "Acta Endocrinologica (Bucharest)",
title = "Case Report of Hand and Foot Skin Changes Resembling PTU-Induced Vasculitis in a Young Male with Diffuse Toxic Goitre",
volume = "19",
number = "3",
pages = "380-385",
doi = "10.4183/aeb.2023.380"
}

Tomasović, M., Sinik, M., Gluvić, Z., Zafirović, S.,& Isenović, E.. (2023). Case Report of Hand and Foot Skin Changes Resembling PTU-Induced Vasculitis in a Young Male with Diffuse Toxic Goitre. in Acta Endocrinologica (Bucharest), 19(3), 380-385.
https://doi.org/10.4183/aeb.2023.380

Tomasović M, Sinik M, Gluvić Z, Zafirović S, Isenović E. Case Report of Hand and Foot Skin Changes Resembling PTU-Induced Vasculitis in a Young Male with Diffuse Toxic Goitre. in Acta Endocrinologica (Bucharest). 2023;19(3):380-385.
doi:10.4183/aeb.2023.380 .

Tomasović, M., Sinik, M., Gluvić, Zoran, Zafirović, Sonja, Isenović, Esma, "Case Report of Hand and Foot Skin Changes Resembling PTU-Induced Vasculitis in a Young Male with Diffuse Toxic Goitre" in Acta Endocrinologica (Bucharest), 19, no. 3 (2023):380-385,
https://doi.org/10.4183/aeb.2023.380 . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Essack, Magbubah
AU  - Šami, Ahmad
AU  - Perry, George
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
AU  - Bajić, Vladan P.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11351
AB  - MicroRNAs (miRNAs) are involved in the regulation of various cellular processes including pathological conditions. MiRNA networks have been extensively researched in age-related degenerative diseases, such as cancer, Alzheimer’s disease (AD), and heart failure. Thus, miRNA has been studied from different approaches, in vivo, in vitro, and in silico including miRNA networks. Networks linking diverse biomedical entities unveil information not readily observable by other means. This work focuses on biological networks related to Breast cancer susceptibility 1 (BRCA1) in AD and breast cancer (BC). Using various bioinformatics approaches, we identified subnetworks common to AD and BC that suggest they are linked. According to our results, miR-107 was identified as a potentially good candidate for both AD and BC treatment (targeting BRCA1/2 and PTEN in both diseases), accompanied by miR-146a and miR-17. The analysis also confirmed the involvement of the miR-17-92 cluster, and miR-124-3p, and highlighted the importance of poorly researched miRNAs such as mir-6785 mir6127, mir-6870, or miR-8485. After filtering the in silico analysis results, we found 49 miRNA molecules that modulate the expression of at least five genes common to both BC and AD. Those 49 miRNAs regulate the expression of 122 genes in AD and 93 genes in BC, from which 26 genes are common genes for AD and BC involved in neuron differentiation and genesis, cell differentiation and migration, regulation of cell cycle, and cancer development. Additionally, the highly enriched pathway was associated with diabetic complications, pointing out possible interplay among molecules underlying BC, AD, and diabetes pathology
T2  - Computational Biology and Chemistry
T1  - MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment
VL  - 106
SP  - 107925
DO  - 10.1016/j.compbiolchem.2023.107925
ER  - 

@article{
author = "Petrović, Nina and Essack, Magbubah and Šami, Ahmad and Perry, George and Gojobori, Takashi and Isenović, Esma R. and Bajić, Vladan P.",
year = "2023",
abstract = "MicroRNAs (miRNAs) are involved in the regulation of various cellular processes including pathological conditions. MiRNA networks have been extensively researched in age-related degenerative diseases, such as cancer, Alzheimer’s disease (AD), and heart failure. Thus, miRNA has been studied from different approaches, in vivo, in vitro, and in silico including miRNA networks. Networks linking diverse biomedical entities unveil information not readily observable by other means. This work focuses on biological networks related to Breast cancer susceptibility 1 (BRCA1) in AD and breast cancer (BC). Using various bioinformatics approaches, we identified subnetworks common to AD and BC that suggest they are linked. According to our results, miR-107 was identified as a potentially good candidate for both AD and BC treatment (targeting BRCA1/2 and PTEN in both diseases), accompanied by miR-146a and miR-17. The analysis also confirmed the involvement of the miR-17-92 cluster, and miR-124-3p, and highlighted the importance of poorly researched miRNAs such as mir-6785 mir6127, mir-6870, or miR-8485. After filtering the in silico analysis results, we found 49 miRNA molecules that modulate the expression of at least five genes common to both BC and AD. Those 49 miRNAs regulate the expression of 122 genes in AD and 93 genes in BC, from which 26 genes are common genes for AD and BC involved in neuron differentiation and genesis, cell differentiation and migration, regulation of cell cycle, and cancer development. Additionally, the highly enriched pathway was associated with diabetic complications, pointing out possible interplay among molecules underlying BC, AD, and diabetes pathology",
journal = "Computational Biology and Chemistry",
title = "MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment",
volume = "106",
pages = "107925",
doi = "10.1016/j.compbiolchem.2023.107925"
}

Petrović, N., Essack, M., Šami, A., Perry, G., Gojobori, T., Isenović, E. R.,& Bajić, V. P.. (2023). MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment. in Computational Biology and Chemistry, 106, 107925.
https://doi.org/10.1016/j.compbiolchem.2023.107925

Petrović N, Essack M, Šami A, Perry G, Gojobori T, Isenović ER, Bajić VP. MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment. in Computational Biology and Chemistry. 2023;106:107925.
doi:10.1016/j.compbiolchem.2023.107925 .

Petrović, Nina, Essack, Magbubah, Šami, Ahmad, Perry, George, Gojobori, Takashi, Isenović, Esma R., Bajić, Vladan P., "MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment" in Computational Biology and Chemistry, 106 (2023):107925,
https://doi.org/10.1016/j.compbiolchem.2023.107925 . .

TY  - JOUR
AU  - Mačvanin, Mirjana T.
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12025
AB  - Diabetes mellitus (DM) refers to a complex cluster of metabolic disorders characterized by hyperglycemia caused by inadequate insulin secretion, insulin resistance, or excessive glucagon secretion. If not correctly treated, the prolonged effects of DM-associated metabolic perturbations lead to systemic vascular complications and cardiovascular disease (CVD), the principal cause of mortality among patients with DM. Given the increase in the global prevalence of diabetes, novel diagnostic and therapeutic procedures are necessary for its effective identification and treatment. Recent findings point to an important role of microRNA (miRNAs) in DM initiation and progression, as well as the occurrence of associated cardiovascular complications. miRNAs are short, highly conserved, single-stranded, non-coding RNAs that contribute to the maintenance of physiological homeostasis through the regulation of crucial processes such as metabolism, cell proliferation, and apoptosis. The increased availability of high-throughput methodologies for identifying and characterizing non-coding RNAs has led to considerable interest in miRNAs as potential biomarkers and therapeutic agents for DM. In this review, we first comprehensively detail the regulatory miRNAs involved in the pathophysiology of DM and diabetic cardiomyopathy (DCMP). Subsequently, we summarize findings regarding the utility of several of these miRNAs as potential prognostic and diagnostic biomarkers for DM and DM-associated CVD. Finally, we evaluate the potential of miRNA-based therapeutic approaches for treating DM and DCMP in the clinical setting.
T2  - Cardiology Plus
T1  - Diabetes and associated cardiovascular complications: The role of microRNAs
VL  - 8
IS  - 3
SP  - 167
EP  - 183
DO  - 10.1097/CP9.0000000000000062
ER  - 

@article{
author = "Mačvanin, Mirjana T. and Isenović, Esma R.",
year = "2023",
abstract = "Diabetes mellitus (DM) refers to a complex cluster of metabolic disorders characterized by hyperglycemia caused by inadequate insulin secretion, insulin resistance, or excessive glucagon secretion. If not correctly treated, the prolonged effects of DM-associated metabolic perturbations lead to systemic vascular complications and cardiovascular disease (CVD), the principal cause of mortality among patients with DM. Given the increase in the global prevalence of diabetes, novel diagnostic and therapeutic procedures are necessary for its effective identification and treatment. Recent findings point to an important role of microRNA (miRNAs) in DM initiation and progression, as well as the occurrence of associated cardiovascular complications. miRNAs are short, highly conserved, single-stranded, non-coding RNAs that contribute to the maintenance of physiological homeostasis through the regulation of crucial processes such as metabolism, cell proliferation, and apoptosis. The increased availability of high-throughput methodologies for identifying and characterizing non-coding RNAs has led to considerable interest in miRNAs as potential biomarkers and therapeutic agents for DM. In this review, we first comprehensively detail the regulatory miRNAs involved in the pathophysiology of DM and diabetic cardiomyopathy (DCMP). Subsequently, we summarize findings regarding the utility of several of these miRNAs as potential prognostic and diagnostic biomarkers for DM and DM-associated CVD. Finally, we evaluate the potential of miRNA-based therapeutic approaches for treating DM and DCMP in the clinical setting.",
journal = "Cardiology Plus",
title = "Diabetes and associated cardiovascular complications: The role of microRNAs",
volume = "8",
number = "3",
pages = "167-183",
doi = "10.1097/CP9.0000000000000062"
}

Mačvanin, M. T.,& Isenović, E. R.. (2023). Diabetes and associated cardiovascular complications: The role of microRNAs. in Cardiology Plus, 8(3), 167-183.
https://doi.org/10.1097/CP9.0000000000000062

Mačvanin MT, Isenović ER. Diabetes and associated cardiovascular complications: The role of microRNAs. in Cardiology Plus. 2023;8(3):167-183.
doi:10.1097/CP9.0000000000000062 .

Mačvanin, Mirjana T., Isenović, Esma R., "Diabetes and associated cardiovascular complications: The role of microRNAs" in Cardiology Plus, 8, no. 3 (2023):167-183,
https://doi.org/10.1097/CP9.0000000000000062 . .

TY  - JOUR
AU  - Khanna, Narendra N
AU  - Singh, Manasvi
AU  - Maindarkar, Mahesh
AU  - Kumar, Ashish
AU  - Johri, Amer M.
AU  - Mentella, Laura
AU  - Laird, John R
AU  - Paraskevas, Kosmas I.
AU  - Ruzsa, Zoltan
AU  - Singh, Narpinder
AU  - Kalra, Mannudeep K.
AU  - Fernandes, Jose Fernandes E.
AU  - Chaturvedi, Seemant
AU  - Nicolaides, Andrew
AU  - Rathore, Vijay
AU  - Singh, Inder
AU  - Teji, Jagjit S.
AU  - Al-Maini, Mostafa
AU  - Isenović, Esma R.
AU  - Viswanathan, Vijay
AU  - Khanna, Puneet
AU  - Fouda, Mostafa M.
AU  - Saba, Luca
AU  - Suri, Jasjit S.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12112
AB  - Cardiovascular disease (CVD) related mortality and morbidity heavily strain society. The relationship between external risk factors and our genetics have not been well established. It is widely acknowledged that environmental influence and individual behaviours play a significant role in CVD vulnerability, leading to the development of polygenic risk scores (PRS). We employed the PRISMA search method to locate pertinent research and literature to extensively review artificial intelligence (AI)-based PRS models for CVD risk prediction. Furthermore, we analyzed and compared conventional vs. AI-based solutions for PRS. We summarized the recent advances in our understanding of the use of AI-based PRS for risk prediction of CVD. Our study proposes three hypotheses: i) Multiple genetic variations and risk factors can be incorporated into AI-based PRS to improve the accuracy of CVD risk predicting. ii) AI-based PRS for CVD circumvents the drawbacks of conventional PRS calculators by incorporating a larger variety of genetic and non-genetic components, allowing for more precise and individualised risk estimations. iii) Using AI approaches, it is possible to significantly reduce the dimensionality of huge genomic datasets, resulting in more accurate and effective disease risk prediction models. Our study highlighted that the AI-PRS model outperformed traditional PRS calculators in predicting CVD risk. Furthermore, using AI-based methods to calculate PRS may increase the precision of risk predictions for CVD and have significant ramifications for individualized prevention and treatment plans.
T2  - Journal of Korean Medical Science
T1  - Polygenic Risk Score for Cardiovascular Diseases in Artificial Intelligence Paradigm: A Review
VL  - 38
IS  - 46
DO  - 10.3346/jkms.2023.38.e395
ER  - 

@article{
author = "Khanna, Narendra N and Singh, Manasvi and Maindarkar, Mahesh and Kumar, Ashish and Johri, Amer M. and Mentella, Laura and Laird, John R and Paraskevas, Kosmas I. and Ruzsa, Zoltan and Singh, Narpinder and Kalra, Mannudeep K. and Fernandes, Jose Fernandes E. and Chaturvedi, Seemant and Nicolaides, Andrew and Rathore, Vijay and Singh, Inder and Teji, Jagjit S. and Al-Maini, Mostafa and Isenović, Esma R. and Viswanathan, Vijay and Khanna, Puneet and Fouda, Mostafa M. and Saba, Luca and Suri, Jasjit S.",
year = "2023",
abstract = "Cardiovascular disease (CVD) related mortality and morbidity heavily strain society. The relationship between external risk factors and our genetics have not been well established. It is widely acknowledged that environmental influence and individual behaviours play a significant role in CVD vulnerability, leading to the development of polygenic risk scores (PRS). We employed the PRISMA search method to locate pertinent research and literature to extensively review artificial intelligence (AI)-based PRS models for CVD risk prediction. Furthermore, we analyzed and compared conventional vs. AI-based solutions for PRS. We summarized the recent advances in our understanding of the use of AI-based PRS for risk prediction of CVD. Our study proposes three hypotheses: i) Multiple genetic variations and risk factors can be incorporated into AI-based PRS to improve the accuracy of CVD risk predicting. ii) AI-based PRS for CVD circumvents the drawbacks of conventional PRS calculators by incorporating a larger variety of genetic and non-genetic components, allowing for more precise and individualised risk estimations. iii) Using AI approaches, it is possible to significantly reduce the dimensionality of huge genomic datasets, resulting in more accurate and effective disease risk prediction models. Our study highlighted that the AI-PRS model outperformed traditional PRS calculators in predicting CVD risk. Furthermore, using AI-based methods to calculate PRS may increase the precision of risk predictions for CVD and have significant ramifications for individualized prevention and treatment plans.",
journal = "Journal of Korean Medical Science",
title = "Polygenic Risk Score for Cardiovascular Diseases in Artificial Intelligence Paradigm: A Review",
volume = "38",
number = "46",
doi = "10.3346/jkms.2023.38.e395"
}

Khanna, N. N., Singh, M., Maindarkar, M., Kumar, A., Johri, A. M., Mentella, L., Laird, J. R., Paraskevas, K. I., Ruzsa, Z., Singh, N., Kalra, M. K., Fernandes, J. F. E., Chaturvedi, S., Nicolaides, A., Rathore, V., Singh, I., Teji, J. S., Al-Maini, M., Isenović, E. R., Viswanathan, V., Khanna, P., Fouda, M. M., Saba, L.,& Suri, J. S.. (2023). Polygenic Risk Score for Cardiovascular Diseases in Artificial Intelligence Paradigm: A Review. in Journal of Korean Medical Science, 38(46).
https://doi.org/10.3346/jkms.2023.38.e395

Khanna NN, Singh M, Maindarkar M, Kumar A, Johri AM, Mentella L, Laird JR, Paraskevas KI, Ruzsa Z, Singh N, Kalra MK, Fernandes JFE, Chaturvedi S, Nicolaides A, Rathore V, Singh I, Teji JS, Al-Maini M, Isenović ER, Viswanathan V, Khanna P, Fouda MM, Saba L, Suri JS. Polygenic Risk Score for Cardiovascular Diseases in Artificial Intelligence Paradigm: A Review. in Journal of Korean Medical Science. 2023;38(46).
doi:10.3346/jkms.2023.38.e395 .

Khanna, Narendra N, Singh, Manasvi, Maindarkar, Mahesh, Kumar, Ashish, Johri, Amer M., Mentella, Laura, Laird, John R, Paraskevas, Kosmas I., Ruzsa, Zoltan, Singh, Narpinder, Kalra, Mannudeep K., Fernandes, Jose Fernandes E., Chaturvedi, Seemant, Nicolaides, Andrew, Rathore, Vijay, Singh, Inder, Teji, Jagjit S., Al-Maini, Mostafa, Isenović, Esma R., Viswanathan, Vijay, Khanna, Puneet, Fouda, Mostafa M., Saba, Luca, Suri, Jasjit S., "Polygenic Risk Score for Cardiovascular Diseases in Artificial Intelligence Paradigm: A Review" in Journal of Korean Medical Science, 38, no. 46 (2023),
https://doi.org/10.3346/jkms.2023.38.e395 . .

TY  - JOUR
AU  - Zarić, Božidarka
AU  - Mačvanin, Mirjana
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10562
AB  - Reactive species are highly-reactive enzymatically, or non-enzymatically produced compounds with important roles in physiological and pathophysiological cellular processes. Although reactive species represent an extensively researched topic in biomedical sciences, many aspects of their roles and functions remain unclear. This review aims to systematically summarize findings regarding the biochemical characteristics of various types of reactive species and specify the localization and mechanisms of their production in cells. In addition, we discuss the specific roles of free radicals in cellular physiology, focusing on the current lines of research that aim to identify the reactive oxygen species-initiated cascades of reactions resulting in adaptive or pathological cellular responses. Finally, we present recent findings regarding the therapeutic modulations of intracellular levels of reactive oxygen species, which may have substantial significance in developing novel agents for treating several diseases.
T2  - The International Journal of Biochemistry and Cell Biology
T1  - Free radicals: Relationship to Human Diseases and Potential Therapeutic applications
VL  - 154
SP  - 106346
DO  - 10.1016/j.biocel.2022.106346
ER  - 

@article{
author = "Zarić, Božidarka and Mačvanin, Mirjana and Isenović, Esma R.",
year = "2023",
abstract = "Reactive species are highly-reactive enzymatically, or non-enzymatically produced compounds with important roles in physiological and pathophysiological cellular processes. Although reactive species represent an extensively researched topic in biomedical sciences, many aspects of their roles and functions remain unclear. This review aims to systematically summarize findings regarding the biochemical characteristics of various types of reactive species and specify the localization and mechanisms of their production in cells. In addition, we discuss the specific roles of free radicals in cellular physiology, focusing on the current lines of research that aim to identify the reactive oxygen species-initiated cascades of reactions resulting in adaptive or pathological cellular responses. Finally, we present recent findings regarding the therapeutic modulations of intracellular levels of reactive oxygen species, which may have substantial significance in developing novel agents for treating several diseases.",
journal = "The International Journal of Biochemistry and Cell Biology",
title = "Free radicals: Relationship to Human Diseases and Potential Therapeutic applications",
volume = "154",
pages = "106346",
doi = "10.1016/j.biocel.2022.106346"
}

Zarić, B., Mačvanin, M.,& Isenović, E. R.. (2023). Free radicals: Relationship to Human Diseases and Potential Therapeutic applications. in The International Journal of Biochemistry and Cell Biology, 154, 106346.
https://doi.org/10.1016/j.biocel.2022.106346

Zarić B, Mačvanin M, Isenović ER. Free radicals: Relationship to Human Diseases and Potential Therapeutic applications. in The International Journal of Biochemistry and Cell Biology. 2023;154:106346.
doi:10.1016/j.biocel.2022.106346 .

Zarić, Božidarka, Mačvanin, Mirjana, Isenović, Esma R., "Free radicals: Relationship to Human Diseases and Potential Therapeutic applications" in The International Journal of Biochemistry and Cell Biology, 154 (2023):106346,
https://doi.org/10.1016/j.biocel.2022.106346 . .