TY  - JOUR
AU  - Nikolić, Dragana
AU  - Djordjevic, P. B.
AU  - Lackovic, V. B.
AU  - Stojiljković, Vesna
AU  - Stanojević, Boban
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5409
AB  - The experiment compared the physiological function (insulin secretory capacity) and membrane integrity of human adult pancreatic islets incubated in culture at 37 degrees C and 24 degrees C. Pancreatic tissue was digested with Collagenase XI, using a non-automated method. Cultures were incubated at 37 degrees C and 24 degrees C. Secretory capacity of the islets is determined by measuring of the stimulation index (SI) on the 3rd and 7th day of cultivation. Membrane integrity of the islets was determined by dithizone staining. Both groups of examined cultures show a slight increase in SI during the incubation. However islets incubated at 24 degrees C show higher SI values than those incubated at 37 degrees C on the 1st, 3rd and 7th day of incubation. And on the first day of incubation, this difference was statistically significant (p LT 0.05). Islets incubated at 37 degrees C showed preservation of membrane integrity, the islets are regular spherical shape, while those incubated at 24 degrees C lose such an organization. During the seven-day cultivation, islets incubated at a standard temperature of 37 degrees C show less preserve physiological functions in relation to cultures incubated at 24 degrees C, but islets incubated at 37 degrees C show more regular morphological forms.
T2  - Journal of Biological Regulators and Homeostatic Agents
T1  - Effect of Low Temperature Cultivation on Insulin Secretory of Human Pancreatic Islets
VL  - 27
IS  - 1
SP  - 35
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5409
ER  - 

@article{
author = "Nikolić, Dragana and Djordjevic, P. B. and Lackovic, V. B. and Stojiljković, Vesna and Stanojević, Boban",
year = "2013",
abstract = "The experiment compared the physiological function (insulin secretory capacity) and membrane integrity of human adult pancreatic islets incubated in culture at 37 degrees C and 24 degrees C. Pancreatic tissue was digested with Collagenase XI, using a non-automated method. Cultures were incubated at 37 degrees C and 24 degrees C. Secretory capacity of the islets is determined by measuring of the stimulation index (SI) on the 3rd and 7th day of cultivation. Membrane integrity of the islets was determined by dithizone staining. Both groups of examined cultures show a slight increase in SI during the incubation. However islets incubated at 24 degrees C show higher SI values than those incubated at 37 degrees C on the 1st, 3rd and 7th day of incubation. And on the first day of incubation, this difference was statistically significant (p LT 0.05). Islets incubated at 37 degrees C showed preservation of membrane integrity, the islets are regular spherical shape, while those incubated at 24 degrees C lose such an organization. During the seven-day cultivation, islets incubated at a standard temperature of 37 degrees C show less preserve physiological functions in relation to cultures incubated at 24 degrees C, but islets incubated at 37 degrees C show more regular morphological forms.",
journal = "Journal of Biological Regulators and Homeostatic Agents",
title = "Effect of Low Temperature Cultivation on Insulin Secretory of Human Pancreatic Islets",
volume = "27",
number = "1",
pages = "35-44",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5409"
}

Nikolić, D., Djordjevic, P. B., Lackovic, V. B., Stojiljković, V.,& Stanojević, B.. (2013). Effect of Low Temperature Cultivation on Insulin Secretory of Human Pancreatic Islets. in Journal of Biological Regulators and Homeostatic Agents, 27(1), 35-44.
https://hdl.handle.net/21.15107/rcub_vinar_5409

Nikolić D, Djordjevic PB, Lackovic VB, Stojiljković V, Stanojević B. Effect of Low Temperature Cultivation on Insulin Secretory of Human Pancreatic Islets. in Journal of Biological Regulators and Homeostatic Agents. 2013;27(1):35-44.
https://hdl.handle.net/21.15107/rcub_vinar_5409 .

Nikolić, Dragana, Djordjevic, P. B., Lackovic, V. B., Stojiljković, Vesna, Stanojević, Boban, "Effect of Low Temperature Cultivation on Insulin Secretory of Human Pancreatic Islets" in Journal of Biological Regulators and Homeostatic Agents, 27, no. 1 (2013):35-44,
https://hdl.handle.net/21.15107/rcub_vinar_5409 .

TY  - JOUR
AU  - Nikolić, Dragana
AU  - Djordjevic, P. B.
AU  - Radak, Đorđe J.
AU  - Lackovic, Vesna B.
AU  - Bajčetić, Miloš I.
AU  - Milić, Gordana A.
AU  - Gostiljac, D.
AU  - Ilic, M.
AU  - Raketic, Nevenka
AU  - Stojiljković, Vesna
AU  - Grubor, Nikica M.
AU  - Jovanovic, M. D.
AU  - Latincic, S. M.
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4653
AB  - In this study we compared the effects of duration of cold ischemia (longer and shorter ischemia) on the yield, viability and preservation of the physiological function and insulin secretion of adult human pancreatic islets in short-term (seven days) culture. Based on the tested parameters, we established that there are no major differences between these two test groups and that the storage and transport of pancreatic tissue in physiological solution at 4 degrees C gives quite satisfactory results.
T2  - Archives of Biological Sciences
T1  - Extended Time of Cold Ischemia and Its Influence on the Physiological Function of Human Adult Pancreatic Islets
VL  - 63
IS  - 4
SP  - 1007
EP  - 1013
DO  - 10.2298/ABS1104007N
ER  - 

@article{
author = "Nikolić, Dragana and Djordjevic, P. B. and Radak, Đorđe J. and Lackovic, Vesna B. and Bajčetić, Miloš I. and Milić, Gordana A. and Gostiljac, D. and Ilic, M. and Raketic, Nevenka and Stojiljković, Vesna and Grubor, Nikica M. and Jovanovic, M. D. and Latincic, S. M.",
year = "2011",
abstract = "In this study we compared the effects of duration of cold ischemia (longer and shorter ischemia) on the yield, viability and preservation of the physiological function and insulin secretion of adult human pancreatic islets in short-term (seven days) culture. Based on the tested parameters, we established that there are no major differences between these two test groups and that the storage and transport of pancreatic tissue in physiological solution at 4 degrees C gives quite satisfactory results.",
journal = "Archives of Biological Sciences",
title = "Extended Time of Cold Ischemia and Its Influence on the Physiological Function of Human Adult Pancreatic Islets",
volume = "63",
number = "4",
pages = "1007-1013",
doi = "10.2298/ABS1104007N"
}

Nikolić, D., Djordjevic, P. B., Radak, Đ. J., Lackovic, V. B., Bajčetić, M. I., Milić, G. A., Gostiljac, D., Ilic, M., Raketic, N., Stojiljković, V., Grubor, N. M., Jovanovic, M. D.,& Latincic, S. M.. (2011). Extended Time of Cold Ischemia and Its Influence on the Physiological Function of Human Adult Pancreatic Islets. in Archives of Biological Sciences, 63(4), 1007-1013.
https://doi.org/10.2298/ABS1104007N

Nikolić D, Djordjevic PB, Radak ĐJ, Lackovic VB, Bajčetić MI, Milić GA, Gostiljac D, Ilic M, Raketic N, Stojiljković V, Grubor NM, Jovanovic MD, Latincic SM. Extended Time of Cold Ischemia and Its Influence on the Physiological Function of Human Adult Pancreatic Islets. in Archives of Biological Sciences. 2011;63(4):1007-1013.
doi:10.2298/ABS1104007N .

Nikolić, Dragana, Djordjevic, P. B., Radak, Đorđe J., Lackovic, Vesna B., Bajčetić, Miloš I., Milić, Gordana A., Gostiljac, D., Ilic, M., Raketic, Nevenka, Stojiljković, Vesna, Grubor, Nikica M., Jovanovic, M. D., Latincic, S. M., "Extended Time of Cold Ischemia and Its Influence on the Physiological Function of Human Adult Pancreatic Islets" in Archives of Biological Sciences, 63, no. 4 (2011):1007-1013,
https://doi.org/10.2298/ABS1104007N . .