TY  - JOUR
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Božanić, Dušan K.
AU  - Bogdanović, Una
AU  - Vodnik, Vesna
AU  - Dimitrijević-Branković, Suzana I.
AU  - Miljković, Miona G.
AU  - Kaščakova, Slavka
AU  - Refregiers, Matthieu
AU  - Đoković, Vladimir
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1608
AB  - The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353 nm] and [370-410 nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells surfaces. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study
VL  - 155
SP  - 341
EP  - 348
DO  - 10.1016/j.colsurfb.2017.04.044
ER  - 

@article{
author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Bogdanović, Una and Vodnik, Vesna and Dimitrijević-Branković, Suzana I. and Miljković, Miona G. and Kaščakova, Slavka and Refregiers, Matthieu and Đoković, Vladimir",
year = "2017",
abstract = "The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353 nm] and [370-410 nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells surfaces. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study",
volume = "155",
pages = "341-348",
doi = "10.1016/j.colsurfb.2017.04.044"
}

Dojčilović, R., Pajović, J. D., Božanić, D. K., Bogdanović, U., Vodnik, V., Dimitrijević-Branković, S. I., Miljković, M. G., Kaščakova, S., Refregiers, M.,& Đoković, V.. (2017). Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study. in Colloids and Surfaces. B: Biointerfaces, 155, 341-348.
https://doi.org/10.1016/j.colsurfb.2017.04.044

Dojčilović R, Pajović JD, Božanić DK, Bogdanović U, Vodnik V, Dimitrijević-Branković SI, Miljković MG, Kaščakova S, Refregiers M, Đoković V. Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study. in Colloids and Surfaces. B: Biointerfaces. 2017;155:341-348.
doi:10.1016/j.colsurfb.2017.04.044 .

Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Bogdanović, Una, Vodnik, Vesna, Dimitrijević-Branković, Suzana I., Miljković, Miona G., Kaščakova, Slavka, Refregiers, Matthieu, Đoković, Vladimir, "Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study" in Colloids and Surfaces. B: Biointerfaces, 155 (2017):341-348,
https://doi.org/10.1016/j.colsurfb.2017.04.044 . .

TY  - JOUR
AU  - Pajović, Jelena D.
AU  - Dojčilović, Radovan
AU  - Božanić, Dušan K.
AU  - Vodnik, Vesna
AU  - Dimitrijević-Branković, Suzana I.
AU  - Kaščakova, Slavka
AU  - Refregiers, Matthieu
AU  - Markelić, Milica
AU  - Đoković, Vladimir
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1107
AB  - Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology.
T2  - Optical and Quantum Electronics
T1  - Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids
VL  - 48
IS  - 6
DO  - 10.1007/s11082-016-0578-y
ER  - 

@article{
author = "Pajović, Jelena D. and Dojčilović, Radovan and Božanić, Dušan K. and Vodnik, Vesna and Dimitrijević-Branković, Suzana I. and Kaščakova, Slavka and Refregiers, Matthieu and Markelić, Milica and Đoković, Vladimir",
year = "2016",
abstract = "Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology.",
journal = "Optical and Quantum Electronics",
title = "Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids",
volume = "48",
number = "6",
doi = "10.1007/s11082-016-0578-y"
}

Pajović, J. D., Dojčilović, R., Božanić, D. K., Vodnik, V., Dimitrijević-Branković, S. I., Kaščakova, S., Refregiers, M., Markelić, M.,& Đoković, V.. (2016). Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids. in Optical and Quantum Electronics, 48(6).
https://doi.org/10.1007/s11082-016-0578-y

Pajović JD, Dojčilović R, Božanić DK, Vodnik V, Dimitrijević-Branković SI, Kaščakova S, Refregiers M, Markelić M, Đoković V. Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids. in Optical and Quantum Electronics. 2016;48(6).
doi:10.1007/s11082-016-0578-y .

Pajović, Jelena D., Dojčilović, Radovan, Božanić, Dušan K., Vodnik, Vesna, Dimitrijević-Branković, Suzana I., Kaščakova, Slavka, Refregiers, Matthieu, Markelić, Milica, Đoković, Vladimir, "Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids" in Optical and Quantum Electronics, 48, no. 6 (2016),
https://doi.org/10.1007/s11082-016-0578-y . .

TY  - JOUR
AU  - Pajović, Jelena D.
AU  - Dojčilović, Radovan
AU  - Božanić, Dušan K.
AU  - Kaščakova, Slavka
AU  - Refregiers, Matthieu
AU  - Dimitrijević-Branković, Suzana I.
AU  - Vodnik, Vesna
AU  - Milosavljević, Aleksandar R.
AU  - Piscopiello, Emanuela
AU  - Luyt, Adriaan S.
AU  - Đoković, Vladimir
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/875
AB  - Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells. (c) 2015 Elsevier B.V. All rights reserved.
PB  - Elsevier
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells
VL  - 135
SP  - 742
EP  - 750
DO  - 10.1016/j.colsurfb.2015.08.050
ER  - 

@article{
author = "Pajović, Jelena D. and Dojčilović, Radovan and Božanić, Dušan K. and Kaščakova, Slavka and Refregiers, Matthieu and Dimitrijević-Branković, Suzana I. and Vodnik, Vesna and Milosavljević, Aleksandar R. and Piscopiello, Emanuela and Luyt, Adriaan S. and Đoković, Vladimir",
year = "2015",
abstract = "Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells. (c) 2015 Elsevier B.V. All rights reserved.",
publisher = "Elsevier",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells",
volume = "135",
pages = "742-750",
doi = "10.1016/j.colsurfb.2015.08.050"
}

Pajović, J. D., Dojčilović, R., Božanić, D. K., Kaščakova, S., Refregiers, M., Dimitrijević-Branković, S. I., Vodnik, V., Milosavljević, A. R., Piscopiello, E., Luyt, A. S.,& Đoković, V.. (2015). Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. in Colloids and Surfaces. B: Biointerfaces
Elsevier., 135, 742-750.
https://doi.org/10.1016/j.colsurfb.2015.08.050

Pajović JD, Dojčilović R, Božanić DK, Kaščakova S, Refregiers M, Dimitrijević-Branković SI, Vodnik V, Milosavljević AR, Piscopiello E, Luyt AS, Đoković V. Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. in Colloids and Surfaces. B: Biointerfaces. 2015;135:742-750.
doi:10.1016/j.colsurfb.2015.08.050 .

Pajović, Jelena D., Dojčilović, Radovan, Božanić, Dušan K., Kaščakova, Slavka, Refregiers, Matthieu, Dimitrijević-Branković, Suzana I., Vodnik, Vesna, Milosavljević, Aleksandar R., Piscopiello, Emanuela, Luyt, Adriaan S., Đoković, Vladimir, "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells" in Colloids and Surfaces. B: Biointerfaces, 135 (2015):742-750,
https://doi.org/10.1016/j.colsurfb.2015.08.050 . .