TY  - JOUR
AU  - Nedeljković, N.
AU  - Banjac, Ana
AU  - Horvat, Anica
AU  - Stojilkovic, M
AU  - Nikezić, Gordana S.
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2702
AB  - Enzymes that hydrolyze extracellular ATP, i.e. ecto-ATPase and ecto-ATP diphosphohydrolase (ATPDase), can be differentiated by ability of the latter to hydrolyze ADP and by slightly different kinetic properties of the two enzymes. Synaptic plasma membrane fractions isolated from rat hippocampus and caudate nucleus exhibit ADP-hydrolyzing activity, as revealed by the enzyme assay, and the presence of ecto-ATPase protein, as revealed by immunological identification on Western blot. These findings indicate that both enzymes are co-expressed in the synaptic membrane compartment of hippocampal and caudate nucleus neurons. Kinetic analysis was performed to determine the relative contribution of each enzyme to the total ATP-hydrolyzing activity, while an inhibition study was carried out in order to exclude the interference of other nonspecific ATPase and phosphatase activities. Based on the kinetic properties, sensitivity to inhibitors and V-ATP/V-ADP ratio of about 2, we concluded that a substantial portion of ATP-hydrolyzing activity in both synaptic membrane preparations can be ascribed to the catalytic action of ATPDase. On the other hand, the highest catalytic efficacy when ATP is the substrate and the greater abundance of ecto-ATPase protein in caudate nucleus preparation suggest that the relative contribution of ecto-ATPase to the total ATP-hydrolyzing activity in the caudate nucleus is higher than in the hippocampus.
T2  - Physiological Research
T1  - Ecto-ATPase and ecto-ATP-diphosphohydrolase are co-localized in rat hippocampal and caudate nucleus synaptic plasma membranes
VL  - 52
IS  - 6
SP  - 797
EP  - 804
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2702
ER  - 

@article{
author = "Nedeljković, N. and Banjac, Ana and Horvat, Anica and Stojilkovic, M and Nikezić, Gordana S.",
year = "2003",
abstract = "Enzymes that hydrolyze extracellular ATP, i.e. ecto-ATPase and ecto-ATP diphosphohydrolase (ATPDase), can be differentiated by ability of the latter to hydrolyze ADP and by slightly different kinetic properties of the two enzymes. Synaptic plasma membrane fractions isolated from rat hippocampus and caudate nucleus exhibit ADP-hydrolyzing activity, as revealed by the enzyme assay, and the presence of ecto-ATPase protein, as revealed by immunological identification on Western blot. These findings indicate that both enzymes are co-expressed in the synaptic membrane compartment of hippocampal and caudate nucleus neurons. Kinetic analysis was performed to determine the relative contribution of each enzyme to the total ATP-hydrolyzing activity, while an inhibition study was carried out in order to exclude the interference of other nonspecific ATPase and phosphatase activities. Based on the kinetic properties, sensitivity to inhibitors and V-ATP/V-ADP ratio of about 2, we concluded that a substantial portion of ATP-hydrolyzing activity in both synaptic membrane preparations can be ascribed to the catalytic action of ATPDase. On the other hand, the highest catalytic efficacy when ATP is the substrate and the greater abundance of ecto-ATPase protein in caudate nucleus preparation suggest that the relative contribution of ecto-ATPase to the total ATP-hydrolyzing activity in the caudate nucleus is higher than in the hippocampus.",
journal = "Physiological Research",
title = "Ecto-ATPase and ecto-ATP-diphosphohydrolase are co-localized in rat hippocampal and caudate nucleus synaptic plasma membranes",
volume = "52",
number = "6",
pages = "797-804",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2702"
}

Nedeljković, N., Banjac, A., Horvat, A., Stojilkovic, M.,& Nikezić, G. S.. (2003). Ecto-ATPase and ecto-ATP-diphosphohydrolase are co-localized in rat hippocampal and caudate nucleus synaptic plasma membranes. in Physiological Research, 52(6), 797-804.
https://hdl.handle.net/21.15107/rcub_vinar_2702

Nedeljković N, Banjac A, Horvat A, Stojilkovic M, Nikezić GS. Ecto-ATPase and ecto-ATP-diphosphohydrolase are co-localized in rat hippocampal and caudate nucleus synaptic plasma membranes. in Physiological Research. 2003;52(6):797-804.
https://hdl.handle.net/21.15107/rcub_vinar_2702 .

Nedeljković, N., Banjac, Ana, Horvat, Anica, Stojilkovic, M, Nikezić, Gordana S., "Ecto-ATPase and ecto-ATP-diphosphohydrolase are co-localized in rat hippocampal and caudate nucleus synaptic plasma membranes" in Physiological Research, 52, no. 6 (2003):797-804,
https://hdl.handle.net/21.15107/rcub_vinar_2702 .

TY  - JOUR
AU  - Ruždijić, Sabera
AU  - Milošević, Jovan
AU  - Popović, Nataša M.
AU  - Pešić, Milica
AU  - Stojilkovic, M
AU  - Kanazir, Selma
AU  - Todorović, Danijela V.
AU  - Ristić-Fira, Aleksandra
AU  - Krstić-Demonacos, Marija
AU  - Kanazir, Selma
AU  - Rakić, Ljubisav
PY  - 2001
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2423
AB  - Tiazofurin and 8-Cl-cAMP are novel antineoplastic agents that have been shown to be effective against various cancer cells in vitro and in vivo. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, apoptosis and downregulation of c-ras and c-myc gene expression. We examined the effects of 8-Cl-cAMP and tiazofurin, either separately or together, on apoptosis induction and c-fos and c-myc expression in melanoma cells. 8-Cl-cAMP and tiazofurin inhibited the growth of melanoma cells in a dose-responsive manner. Whether used separately or together, each agent induced apoptotic cell death. Apoptosis was accompanied by a marked inhibition of c-fos and c-mye gene expression. RT-PCR analysis showed that 8-Cl-cAMP, together with tiazofurin, promoted 61% and 75% decreases of c-myc and c-fos expression in melanoma cells respectively. These results clearly indicate that the combination of 8-Cl-cAMP and tiazofurin could provide a promising therapeutic approach for melanoma treatment.
T2  - Jugoslovenska Medicinska Biohemija
T1  - Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells
VL  - 20
IS  - 1
SP  - 9
EP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2423
ER  - 

@article{
author = "Ruždijić, Sabera and Milošević, Jovan and Popović, Nataša M. and Pešić, Milica and Stojilkovic, M and Kanazir, Selma and Todorović, Danijela V. and Ristić-Fira, Aleksandra and Krstić-Demonacos, Marija and Kanazir, Selma and Rakić, Ljubisav",
year = "2001",
abstract = "Tiazofurin and 8-Cl-cAMP are novel antineoplastic agents that have been shown to be effective against various cancer cells in vitro and in vivo. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, apoptosis and downregulation of c-ras and c-myc gene expression. We examined the effects of 8-Cl-cAMP and tiazofurin, either separately or together, on apoptosis induction and c-fos and c-myc expression in melanoma cells. 8-Cl-cAMP and tiazofurin inhibited the growth of melanoma cells in a dose-responsive manner. Whether used separately or together, each agent induced apoptotic cell death. Apoptosis was accompanied by a marked inhibition of c-fos and c-mye gene expression. RT-PCR analysis showed that 8-Cl-cAMP, together with tiazofurin, promoted 61% and 75% decreases of c-myc and c-fos expression in melanoma cells respectively. These results clearly indicate that the combination of 8-Cl-cAMP and tiazofurin could provide a promising therapeutic approach for melanoma treatment.",
journal = "Jugoslovenska Medicinska Biohemija",
title = "Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells",
volume = "20",
number = "1",
pages = "9-18",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2423"
}

Ruždijić, S., Milošević, J., Popović, N. M., Pešić, M., Stojilkovic, M., Kanazir, S., Todorović, D. V., Ristić-Fira, A., Krstić-Demonacos, M., Kanazir, S.,& Rakić, L.. (2001). Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells. in Jugoslovenska Medicinska Biohemija, 20(1), 9-18.
https://hdl.handle.net/21.15107/rcub_vinar_2423

Ruždijić S, Milošević J, Popović NM, Pešić M, Stojilkovic M, Kanazir S, Todorović DV, Ristić-Fira A, Krstić-Demonacos M, Kanazir S, Rakić L. Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells. in Jugoslovenska Medicinska Biohemija. 2001;20(1):9-18.
https://hdl.handle.net/21.15107/rcub_vinar_2423 .

Ruždijić, Sabera, Milošević, Jovan, Popović, Nataša M., Pešić, Milica, Stojilkovic, M, Kanazir, Selma, Todorović, Danijela V., Ristić-Fira, Aleksandra, Krstić-Demonacos, Marija, Kanazir, Selma, Rakić, Ljubisav, "Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells" in Jugoslovenska Medicinska Biohemija, 20, no. 1 (2001):9-18,
https://hdl.handle.net/21.15107/rcub_vinar_2423 .