TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Stanimirovic, Z.
AU  - Stevanović, Jevrosima
AU  - Milićević, Zorka T.
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3998
AB  - Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono- and/or combinational therapies for cancer treatment.
T2  - Archives of Biological Sciences
T1  - The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro
VL  - 62
IS  - 1
SP  - 63
EP  - 74
DO  - 10.2298/ABS1001063B
ER  - 

@article{
author = "Bajić, Vladan P. and Stanimirovic, Z. and Stevanović, Jevrosima and Milićević, Zorka T. and Živković, Lada and Spremo-Potparević, Biljana",
year = "2010",
abstract = "Premature centromere division (PCD) can be viewed as a manifestation of chromosome instability. In order to evaluate the ability of Paclitaxel (Ptx) and Cycloheximide (Cy) to induce PCD we used a cytokinesis block micronucleus assay (CBMN), fluorescent in situ hybridization (FISH), and the chromosome aberration (CA) assay in human peripheral blood lymphocytes. Results showed that Ptx can induce PCD alone or in combination with Cy. These findings call us to pay more attention to PCD as a parameter of genotoxicity in the pre-clinical research of mono- and/or combinational therapies for cancer treatment.",
journal = "Archives of Biological Sciences",
title = "The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro",
volume = "62",
number = "1",
pages = "63-74",
doi = "10.2298/ABS1001063B"
}

Bajić, V. P., Stanimirovic, Z., Stevanović, J., Milićević, Z. T., Živković, L.,& Spremo-Potparević, B.. (2010). The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro. in Archives of Biological Sciences, 62(1), 63-74.
https://doi.org/10.2298/ABS1001063B

Bajić VP, Stanimirovic Z, Stevanović J, Milićević ZT, Živković L, Spremo-Potparević B. The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro. in Archives of Biological Sciences. 2010;62(1):63-74.
doi:10.2298/ABS1001063B .

Bajić, Vladan P., Stanimirovic, Z., Stevanović, Jevrosima, Milićević, Zorka T., Živković, Lada, Spremo-Potparević, Biljana, "The Effect of Paclitaxel Alone and in Combination with Cycloheximide on the Frequency of Premature Centromere Division in Vitro" in Archives of Biological Sciences, 62, no. 1 (2010):63-74,
https://doi.org/10.2298/ABS1001063B . .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Stanimirovic, Z.
AU  - Stevanović, Jasmina
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Milićević, Zorka T.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3670
AB  - Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3, 5 cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assail (CA) on human peripheral blood lymphocytes of healthy individuals and by hone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 pin) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p) injection of 8-Cl-cAMP in doses of 10, 80 and 15 0 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.
T2  - Journal of BUON
T1  - Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes
VL  - 14
IS  - 1
SP  - 71
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3670
ER  - 

@article{
author = "Bajić, Vladan P. and Stanimirovic, Z. and Stevanović, Jasmina and Spremo-Potparević, Biljana and Živković, Lada and Milićević, Zorka T.",
year = "2009",
abstract = "Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3, 5 cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assail (CA) on human peripheral blood lymphocytes of healthy individuals and by hone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 pin) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p) injection of 8-Cl-cAMP in doses of 10, 80 and 15 0 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.",
journal = "Journal of BUON",
title = "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes",
volume = "14",
number = "1",
pages = "71-77",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3670"
}

Bajić, V. P., Stanimirovic, Z., Stevanović, J., Spremo-Potparević, B., Živković, L.,& Milićević, Z. T.. (2009). Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON, 14(1), 71-77.
https://hdl.handle.net/21.15107/rcub_vinar_3670

Bajić VP, Stanimirovic Z, Stevanović J, Spremo-Potparević B, Živković L, Milićević ZT. Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON. 2009;14(1):71-77.
https://hdl.handle.net/21.15107/rcub_vinar_3670 .

Bajić, Vladan P., Stanimirovic, Z., Stevanović, Jasmina, Spremo-Potparević, Biljana, Živković, Lada, Milićević, Zorka T., "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes" in Journal of BUON, 14, no. 1 (2009):71-77,
https://hdl.handle.net/21.15107/rcub_vinar_3670 .