TY  - JOUR
AU  - Obradović, Milan M.
AU  - Stewart, Alan J.
AU  - Pitt, Samantha J.
AU  - Labudović-Borović, Milica
AU  - Sudar, Emina
AU  - Petrovic, Voin
AU  - Zafirović, Sonja
AU  - Maravić-Stojković, Vera
AU  - Vasić, Vesna M.
AU  - Isenović, Esma R.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5989
AB  - In this study the in vivo effects of estradiol in regulating Na+/K+-ATPase function in rat heart was studied. Adult male Wistar rats were treated with estradiol (40 mu g/kg, i.p.) and after 24 h the animals were sacrificed and the heart excised. Following estradiol administration, cardiac Na+/K(+)ATPase activity, expression of the alpha l subunit, and phosphorylation of the al subunit were significantly increased. These animals also had significantly decreased levels of digoxin-like immunoreactive factor(s). Na+ levels were also significantly reduced but to a level that was still within the normal physiological range, highlighting the ability of the Na+/K+-ATPase to balance the ionic composition following treatment with estradiol. Estradiol treated rats also showed increased phosphorylation of protein kinase B (Akt), and extracellular-signal-regulated kinase 1/2 (ERK1/2). We therefore suggest a role for Akt and/or ERK1/2 in estradiol-mediated regulation of cardiac Na+/K+-ATPase expression and activity in rat heart. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
T2  - Molecular and Cellular Endocrinology
T1  - In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart
VL  - 388
IS  - 1-2
SP  - 58
EP  - 68
DO  - 10.1016/j.mce.2014.03.005
ER  - 

@article{
author = "Obradović, Milan M. and Stewart, Alan J. and Pitt, Samantha J. and Labudović-Borović, Milica and Sudar, Emina and Petrovic, Voin and Zafirović, Sonja and Maravić-Stojković, Vera and Vasić, Vesna M. and Isenović, Esma R.",
year = "2014",
abstract = "In this study the in vivo effects of estradiol in regulating Na+/K+-ATPase function in rat heart was studied. Adult male Wistar rats were treated with estradiol (40 mu g/kg, i.p.) and after 24 h the animals were sacrificed and the heart excised. Following estradiol administration, cardiac Na+/K(+)ATPase activity, expression of the alpha l subunit, and phosphorylation of the al subunit were significantly increased. These animals also had significantly decreased levels of digoxin-like immunoreactive factor(s). Na+ levels were also significantly reduced but to a level that was still within the normal physiological range, highlighting the ability of the Na+/K+-ATPase to balance the ionic composition following treatment with estradiol. Estradiol treated rats also showed increased phosphorylation of protein kinase B (Akt), and extracellular-signal-regulated kinase 1/2 (ERK1/2). We therefore suggest a role for Akt and/or ERK1/2 in estradiol-mediated regulation of cardiac Na+/K+-ATPase expression and activity in rat heart. (C) 2014 Elsevier Ireland Ltd. All rights reserved.",
journal = "Molecular and Cellular Endocrinology",
title = "In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart",
volume = "388",
number = "1-2",
pages = "58-68",
doi = "10.1016/j.mce.2014.03.005"
}

Obradović, M. M., Stewart, A. J., Pitt, S. J., Labudović-Borović, M., Sudar, E., Petrovic, V., Zafirović, S., Maravić-Stojković, V., Vasić, V. M.,& Isenović, E. R.. (2014). In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart. in Molecular and Cellular Endocrinology, 388(1-2), 58-68.
https://doi.org/10.1016/j.mce.2014.03.005

Obradović MM, Stewart AJ, Pitt SJ, Labudović-Borović M, Sudar E, Petrovic V, Zafirović S, Maravić-Stojković V, Vasić VM, Isenović ER. In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart. in Molecular and Cellular Endocrinology. 2014;388(1-2):58-68.
doi:10.1016/j.mce.2014.03.005 .

Obradović, Milan M., Stewart, Alan J., Pitt, Samantha J., Labudović-Borović, Milica, Sudar, Emina, Petrovic, Voin, Zafirović, Sonja, Maravić-Stojković, Vera, Vasić, Vesna M., Isenović, Esma R., "In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart" in Molecular and Cellular Endocrinology, 388, no. 1-2 (2014):58-68,
https://doi.org/10.1016/j.mce.2014.03.005 . .

TY  - JOUR
AU  - Petrović, Voin
AU  - Petrović, Sandra
AU  - Joksić, Gordana
AU  - Savić, Jasmina
AU  - Čolović, Mirjana B.
AU  - Cinellu, Maria Agostina
AU  - Massai, Lara
AU  - Messori, Luigi
AU  - Vasić, Vesna M.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/140
AB  - Na+/K+-ATPase is in charge of maintaining the ionic and osmotic intracellular balance by using ATP as an energy source to drive excess Na+ ions out of the cell in exchange for K+ ions. We explored whether three representative cytotoxic gold(III) compounds might interfere with Na+/K+-ATPase and cause its inhibition at pharmacologically relevant concentrations. The tested complexes were [Au(bipy)(OH)(2)][PF6] (bipy = 2,2-bipyridine), [Au (py(dmb)-H)(CH3COO)(2)] (py(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-pyridine), and [Au(bipy(dmb)-H)(OH)][PF6] (bipy(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-2,2-bipyridine). We found that all of them caused a pronounced and similar inhibition of Na+/K+-ATPase activity. Inhibition was found to be non-competitive and reversible. Remarkably, treatment with cysteine resulted in reversal or prevention of Na+/K+-ATPase inhibition. It is very likely that the described effects may contribute to the overall cytotoxic profile of these gold complexes. (C) 2014 Elsevier Inc. All rights reserved.
T2  - Journal of Inorganic Biochemistry
T1  - Inhibition of Na+/K+-ATPase and cytotoxicity of a few selected gold(III) complexes
VL  - 140
SP  - 228
EP  - 235
DO  - 10.1016/j.jinorgbio.2014.07.015
ER  - 

@article{
author = "Petrović, Voin and Petrović, Sandra and Joksić, Gordana and Savić, Jasmina and Čolović, Mirjana B. and Cinellu, Maria Agostina and Massai, Lara and Messori, Luigi and Vasić, Vesna M.",
year = "2014",
abstract = "Na+/K+-ATPase is in charge of maintaining the ionic and osmotic intracellular balance by using ATP as an energy source to drive excess Na+ ions out of the cell in exchange for K+ ions. We explored whether three representative cytotoxic gold(III) compounds might interfere with Na+/K+-ATPase and cause its inhibition at pharmacologically relevant concentrations. The tested complexes were [Au(bipy)(OH)(2)][PF6] (bipy = 2,2-bipyridine), [Au (py(dmb)-H)(CH3COO)(2)] (py(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-pyridine), and [Au(bipy(dmb)-H)(OH)][PF6] (bipy(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-2,2-bipyridine). We found that all of them caused a pronounced and similar inhibition of Na+/K+-ATPase activity. Inhibition was found to be non-competitive and reversible. Remarkably, treatment with cysteine resulted in reversal or prevention of Na+/K+-ATPase inhibition. It is very likely that the described effects may contribute to the overall cytotoxic profile of these gold complexes. (C) 2014 Elsevier Inc. All rights reserved.",
journal = "Journal of Inorganic Biochemistry",
title = "Inhibition of Na+/K+-ATPase and cytotoxicity of a few selected gold(III) complexes",
volume = "140",
pages = "228-235",
doi = "10.1016/j.jinorgbio.2014.07.015"
}

Petrović, V., Petrović, S., Joksić, G., Savić, J., Čolović, M. B., Cinellu, M. A., Massai, L., Messori, L.,& Vasić, V. M.. (2014). Inhibition of Na+/K+-ATPase and cytotoxicity of a few selected gold(III) complexes. in Journal of Inorganic Biochemistry, 140, 228-235.
https://doi.org/10.1016/j.jinorgbio.2014.07.015

Petrović V, Petrović S, Joksić G, Savić J, Čolović MB, Cinellu MA, Massai L, Messori L, Vasić VM. Inhibition of Na+/K+-ATPase and cytotoxicity of a few selected gold(III) complexes. in Journal of Inorganic Biochemistry. 2014;140:228-235.
doi:10.1016/j.jinorgbio.2014.07.015 .

Petrović, Voin, Petrović, Sandra, Joksić, Gordana, Savić, Jasmina, Čolović, Mirjana B., Cinellu, Maria Agostina, Massai, Lara, Messori, Luigi, Vasić, Vesna M., "Inhibition of Na+/K+-ATPase and cytotoxicity of a few selected gold(III) complexes" in Journal of Inorganic Biochemistry, 140 (2014):228-235,
https://doi.org/10.1016/j.jinorgbio.2014.07.015 . .

TY  - JOUR
AU  - Petrovic, Voin
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Vujačić, Ana V.
AU  - Petrović, Sandra
AU  - Joksić, Gordana
AU  - Bugarčić, Živadin D.
AU  - Vasić, Vesna M.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5539
AB  - The in vitro influence of gold(III) complexes, H[AuCl4], [Au(DMSO)(2)Cl-2]Cl and [Au(bipy)Cl-2]Cl (bipy = 2,2-bipyridine), upon commercially available Na+ /K+ ATPase activity, purified from porcine brain cortex, was investigated. Additionally, the complexes were tested on human lymphocytes, and incidence of micronuclei and cell proliferation index was determined. Concentration-dependent inhibition of the enzyme for all three compounds was obtained, but with differing potencies. Calculated IC50 from Hill analysis were (in M): 5.75 x 10(-7), 5.50 x 10(-6) and 3.98 x 10(-5), for H[AuCl4], [Au(DMSO)(2)Cl-2]Cl and [Au(bipy)Cl-2]Cl, respectively, while Hill coefficient values, n, were above 1 in all cases. This inhibition can be prevented using -SH donating ligands such as L-Cys and glutathione, and these ligands can also cause a recovery of the enzyme activity after the induced inhibition. Kinetic analysis demonstrated that each of the studied gold(III) complexes affects Na+ /K+ ATPase reducing maximum enzymatic velocity, V-max, but not significantly changing the affinity for the substrate (K-M value), implying a noncompetitive mode of the interaction. Furthermore, among investigated gold(III) complexes, the [Au(bipy)Cl-2]Cl complex exhibits a strong cytotoxic effect on human lymphocytes, which suggests its potential for use in antitumor therapy. (C(C) 2013 Elsevier Inc. All rights reserved.
T2  - Journal of Inorganic Biochemistry
T1  - In vitro effects of some gold complexes on Na+/K+ ATPase activity and cell proliferation
VL  - 124
SP  - 35
EP  - 41
DO  - 10.1016/j.jinorgbio.2013.03.013
ER  - 

@article{
author = "Petrovic, Voin and Čolović, Mirjana B. and Krstić, Danijela Z. and Vujačić, Ana V. and Petrović, Sandra and Joksić, Gordana and Bugarčić, Živadin D. and Vasić, Vesna M.",
year = "2013",
abstract = "The in vitro influence of gold(III) complexes, H[AuCl4], [Au(DMSO)(2)Cl-2]Cl and [Au(bipy)Cl-2]Cl (bipy = 2,2-bipyridine), upon commercially available Na+ /K+ ATPase activity, purified from porcine brain cortex, was investigated. Additionally, the complexes were tested on human lymphocytes, and incidence of micronuclei and cell proliferation index was determined. Concentration-dependent inhibition of the enzyme for all three compounds was obtained, but with differing potencies. Calculated IC50 from Hill analysis were (in M): 5.75 x 10(-7), 5.50 x 10(-6) and 3.98 x 10(-5), for H[AuCl4], [Au(DMSO)(2)Cl-2]Cl and [Au(bipy)Cl-2]Cl, respectively, while Hill coefficient values, n, were above 1 in all cases. This inhibition can be prevented using -SH donating ligands such as L-Cys and glutathione, and these ligands can also cause a recovery of the enzyme activity after the induced inhibition. Kinetic analysis demonstrated that each of the studied gold(III) complexes affects Na+ /K+ ATPase reducing maximum enzymatic velocity, V-max, but not significantly changing the affinity for the substrate (K-M value), implying a noncompetitive mode of the interaction. Furthermore, among investigated gold(III) complexes, the [Au(bipy)Cl-2]Cl complex exhibits a strong cytotoxic effect on human lymphocytes, which suggests its potential for use in antitumor therapy. (C(C) 2013 Elsevier Inc. All rights reserved.",
journal = "Journal of Inorganic Biochemistry",
title = "In vitro effects of some gold complexes on Na+/K+ ATPase activity and cell proliferation",
volume = "124",
pages = "35-41",
doi = "10.1016/j.jinorgbio.2013.03.013"
}

Petrovic, V., Čolović, M. B., Krstić, D. Z., Vujačić, A. V., Petrović, S., Joksić, G., Bugarčić, Ž. D.,& Vasić, V. M.. (2013). In vitro effects of some gold complexes on Na+/K+ ATPase activity and cell proliferation. in Journal of Inorganic Biochemistry, 124, 35-41.
https://doi.org/10.1016/j.jinorgbio.2013.03.013

Petrovic V, Čolović MB, Krstić DZ, Vujačić AV, Petrović S, Joksić G, Bugarčić ŽD, Vasić VM. In vitro effects of some gold complexes on Na+/K+ ATPase activity and cell proliferation. in Journal of Inorganic Biochemistry. 2013;124:35-41.
doi:10.1016/j.jinorgbio.2013.03.013 .

Petrovic, Voin, Čolović, Mirjana B., Krstić, Danijela Z., Vujačić, Ana V., Petrović, Sandra, Joksić, Gordana, Bugarčić, Živadin D., Vasić, Vesna M., "In vitro effects of some gold complexes on Na+/K+ ATPase activity and cell proliferation" in Journal of Inorganic Biochemistry, 124 (2013):35-41,
https://doi.org/10.1016/j.jinorgbio.2013.03.013 . .

TY  - JOUR
AU  - Petrovic, Voin
AU  - Vodnik, Vesna
AU  - Stanojević, Ivana
AU  - Rakočević, Zlatko Lj.
AU  - Vasić, Vesna M.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4934
AB  - The aim of the work was to investigate the interaction between borate capped gold nanoparticles (NPs) and the rat brain synaptosomal plasma membranes (SPM), as well as the effects of these NPs on SPM Na+/K+ - ATPase and Mg2+-ATPase activity. The changes in the UV-vis spectra of NPs and SPM assembly suggested the agglomeration and precipitation of NPs. FTIR measurements indicated that both protein -SH and -NH2 groups and positively charged membrane fragments were implicated in the adhesion of SPM to the surface of NPs. AFM showed an increase in the particularization of the SPM material after mixing with gold NPs. Influence of gold NPs on Na+/K+-ATPase and Mg2+-ATPase activity was investigated as the function of NPs and protein concentration, preincubation time and also in the presence of various concentrations of ouabain, the specific enzyme inhibitor. NPs induced the stimulation of Na+/K+-ATPase activity for more than 100%, since Mg2+-ATPase activity reminded unaffected. We propose that this stimulation of enzyme activity was a consequence of an increase of the active surface of membranes.
T2  - Digest Journal of Nanomaterials and Biostructures
T1  - Interaction of Gold Nanoparticles with Rat Brain Synaptosomal Plasma Membrane Na+/K+ - Atpase and Mg2+-Atpase
VL  - 7
IS  - 2
SP  - 423
EP  - 433
UR  - https://hdl.handle.net/21.15107/rcub_vinar_4934
ER  - 

@article{
author = "Petrovic, Voin and Vodnik, Vesna and Stanojević, Ivana and Rakočević, Zlatko Lj. and Vasić, Vesna M.",
year = "2012",
abstract = "The aim of the work was to investigate the interaction between borate capped gold nanoparticles (NPs) and the rat brain synaptosomal plasma membranes (SPM), as well as the effects of these NPs on SPM Na+/K+ - ATPase and Mg2+-ATPase activity. The changes in the UV-vis spectra of NPs and SPM assembly suggested the agglomeration and precipitation of NPs. FTIR measurements indicated that both protein -SH and -NH2 groups and positively charged membrane fragments were implicated in the adhesion of SPM to the surface of NPs. AFM showed an increase in the particularization of the SPM material after mixing with gold NPs. Influence of gold NPs on Na+/K+-ATPase and Mg2+-ATPase activity was investigated as the function of NPs and protein concentration, preincubation time and also in the presence of various concentrations of ouabain, the specific enzyme inhibitor. NPs induced the stimulation of Na+/K+-ATPase activity for more than 100%, since Mg2+-ATPase activity reminded unaffected. We propose that this stimulation of enzyme activity was a consequence of an increase of the active surface of membranes.",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "Interaction of Gold Nanoparticles with Rat Brain Synaptosomal Plasma Membrane Na+/K+ - Atpase and Mg2+-Atpase",
volume = "7",
number = "2",
pages = "423-433",
url = "https://hdl.handle.net/21.15107/rcub_vinar_4934"
}

Petrovic, V., Vodnik, V., Stanojević, I., Rakočević, Z. Lj.,& Vasić, V. M.. (2012). Interaction of Gold Nanoparticles with Rat Brain Synaptosomal Plasma Membrane Na+/K+ - Atpase and Mg2+-Atpase. in Digest Journal of Nanomaterials and Biostructures, 7(2), 423-433.
https://hdl.handle.net/21.15107/rcub_vinar_4934

Petrovic V, Vodnik V, Stanojević I, Rakočević ZL, Vasić VM. Interaction of Gold Nanoparticles with Rat Brain Synaptosomal Plasma Membrane Na+/K+ - Atpase and Mg2+-Atpase. in Digest Journal of Nanomaterials and Biostructures. 2012;7(2):423-433.
https://hdl.handle.net/21.15107/rcub_vinar_4934 .

Petrovic, Voin, Vodnik, Vesna, Stanojević, Ivana, Rakočević, Zlatko Lj., Vasić, Vesna M., "Interaction of Gold Nanoparticles with Rat Brain Synaptosomal Plasma Membrane Na+/K+ - Atpase and Mg2+-Atpase" in Digest Journal of Nanomaterials and Biostructures, 7, no. 2 (2012):423-433,
https://hdl.handle.net/21.15107/rcub_vinar_4934 .