TY  - JOUR
AU  - Guševac Stojanović, Ivana
AU  - Drakulić, Dunja
AU  - Todorović, Ana
AU  - Martinović, Jelena
AU  - Filipović, Nenad
AU  - Stojanović, Zoran
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13127
AB  - While the effects of chronic exposure to microplastic particles (MPs) are extensively studied, the outcomes of a single treatment have received relatively less attention. To investigate MPs’ potential acute toxicity, including their impact on general health status (victual consumption, sensorimotor deficits, and clinical toxicity signs) and serum biochemical parameters (markers of organ/tissue function and oxidative stress indicators), we administered thoroughly characterized MPs (1.4, 35, or 125 mg/kg), generated from polyethylene terephthalate (PET) bottles, to adult male Wistar rats via oral gavage. The MPs’ short-term effects were assessed with well-established tests and methods. The results point to the absence of sensorimotor deficits and clinical toxicity signs, while levels of markers of liver, heart, and kidney function were altered in all MP groups. Decreased victual consumption and increased levels of oxidative stress indicators were evident following treatment with the two higher MP doses. Presented data indicate that examined MPs are able to initiate the development of local changes in tissues and organs within a short time frame, potentially leading to their damage and dysfunction. This study may increase the awareness of the detrimental effects of plastic contamination, as even a single exposure to MPs may provoke adverse health outcomes.
T2  - Toxics
T1  - Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats
VL  - 12
IS  - 3
SP  - 167
DO  - 10.3390/toxics12030167
ER  - 

@article{
author = "Guševac Stojanović, Ivana and Drakulić, Dunja and Todorović, Ana and Martinović, Jelena and Filipović, Nenad and Stojanović, Zoran",
year = "2024",
abstract = "While the effects of chronic exposure to microplastic particles (MPs) are extensively studied, the outcomes of a single treatment have received relatively less attention. To investigate MPs’ potential acute toxicity, including their impact on general health status (victual consumption, sensorimotor deficits, and clinical toxicity signs) and serum biochemical parameters (markers of organ/tissue function and oxidative stress indicators), we administered thoroughly characterized MPs (1.4, 35, or 125 mg/kg), generated from polyethylene terephthalate (PET) bottles, to adult male Wistar rats via oral gavage. The MPs’ short-term effects were assessed with well-established tests and methods. The results point to the absence of sensorimotor deficits and clinical toxicity signs, while levels of markers of liver, heart, and kidney function were altered in all MP groups. Decreased victual consumption and increased levels of oxidative stress indicators were evident following treatment with the two higher MP doses. Presented data indicate that examined MPs are able to initiate the development of local changes in tissues and organs within a short time frame, potentially leading to their damage and dysfunction. This study may increase the awareness of the detrimental effects of plastic contamination, as even a single exposure to MPs may provoke adverse health outcomes.",
journal = "Toxics",
title = "Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats",
volume = "12",
number = "3",
pages = "167",
doi = "10.3390/toxics12030167"
}

Guševac Stojanović, I., Drakulić, D., Todorović, A., Martinović, J., Filipović, N.,& Stojanović, Z.. (2024). Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats. in Toxics, 12(3), 167.
https://doi.org/10.3390/toxics12030167

Guševac Stojanović I, Drakulić D, Todorović A, Martinović J, Filipović N, Stojanović Z. Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats. in Toxics. 2024;12(3):167.
doi:10.3390/toxics12030167 .

Guševac Stojanović, Ivana, Drakulić, Dunja, Todorović, Ana, Martinović, Jelena, Filipović, Nenad, Stojanović, Zoran, "Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats" in Toxics, 12, no. 3 (2024):167,
https://doi.org/10.3390/toxics12030167 . .

TY  - JOUR
AU  - Todorović, Ana
AU  - Bobić, Katarina
AU  - Veljković, Filip
AU  - Pejić, Snežana
AU  - Glumac, Sofija
AU  - Stanković, Sanja
AU  - Milovanović, Tijana
AU  - Vukoje, Ivana
AU  - Nedeljković, Jovan
AU  - Radojević Škodrić, Sanja
AU  - Pajović, Snežana B.
AU  - Drakulić, Dunja
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13090
AB  - Nanoparticles (NPs), a distinct class of particles ranging in size from 1 to 100 nm, are one of the most promising technologies of the 21st century, and titanium dioxide NPs (TiO2 NPs) are among the most widely produced and used NPs globally. The increased application of TiO2 NPs raises concerns regarding their global safety and risks of exposure. Many animal studies have reported the accumulation of TiO2 NPs in female reproductive organs; however, evidence of the resultant toxicity remains ambiguous. Since the surface area and chemical modifications of NPs can significantly change their cytotoxicity, we aimed to compare the toxic effects of pristine TiO2 powder with surface-modified TiO2 powders with salicylic acid (TiO2/SA) and 5-aminosalicylic acid (TiO2/5-ASA) on the ovaries, oviducts, and uterus on the 14th day following acute oral treatment. The results, based on alterations in food and water intake, body mass, organ-to-body mass ratio, hormonal status, histological features of tissues of interest, and antioxidant parameters, suggest that the modification with 5-ASA can mitigate some of the observed toxic effects of TiO2 powder and encourage future investigations to create NPs that can potentially reduce the harmful effects of TiO2 NPs while preserving their positive impacts.
T2  - Antioxidants
T1  - Comparable Toxicity of Surface-Modified TiO2 Nanoparticles: An In Vivo Experimental Study on Reproductive Toxicity in Rats
VL  - 13
IS  - 2
SP  - 231
DO  - 10.3390/antiox13020231
ER  - 

@article{
author = "Todorović, Ana and Bobić, Katarina and Veljković, Filip and Pejić, Snežana and Glumac, Sofija and Stanković, Sanja and Milovanović, Tijana and Vukoje, Ivana and Nedeljković, Jovan and Radojević Škodrić, Sanja and Pajović, Snežana B. and Drakulić, Dunja",
year = "2024",
abstract = "Nanoparticles (NPs), a distinct class of particles ranging in size from 1 to 100 nm, are one of the most promising technologies of the 21st century, and titanium dioxide NPs (TiO2 NPs) are among the most widely produced and used NPs globally. The increased application of TiO2 NPs raises concerns regarding their global safety and risks of exposure. Many animal studies have reported the accumulation of TiO2 NPs in female reproductive organs; however, evidence of the resultant toxicity remains ambiguous. Since the surface area and chemical modifications of NPs can significantly change their cytotoxicity, we aimed to compare the toxic effects of pristine TiO2 powder with surface-modified TiO2 powders with salicylic acid (TiO2/SA) and 5-aminosalicylic acid (TiO2/5-ASA) on the ovaries, oviducts, and uterus on the 14th day following acute oral treatment. The results, based on alterations in food and water intake, body mass, organ-to-body mass ratio, hormonal status, histological features of tissues of interest, and antioxidant parameters, suggest that the modification with 5-ASA can mitigate some of the observed toxic effects of TiO2 powder and encourage future investigations to create NPs that can potentially reduce the harmful effects of TiO2 NPs while preserving their positive impacts.",
journal = "Antioxidants",
title = "Comparable Toxicity of Surface-Modified TiO2 Nanoparticles: An In Vivo Experimental Study on Reproductive Toxicity in Rats",
volume = "13",
number = "2",
pages = "231",
doi = "10.3390/antiox13020231"
}

Todorović, A., Bobić, K., Veljković, F., Pejić, S., Glumac, S., Stanković, S., Milovanović, T., Vukoje, I., Nedeljković, J., Radojević Škodrić, S., Pajović, S. B.,& Drakulić, D.. (2024). Comparable Toxicity of Surface-Modified TiO2 Nanoparticles: An In Vivo Experimental Study on Reproductive Toxicity in Rats. in Antioxidants, 13(2), 231.
https://doi.org/10.3390/antiox13020231

Todorović A, Bobić K, Veljković F, Pejić S, Glumac S, Stanković S, Milovanović T, Vukoje I, Nedeljković J, Radojević Škodrić S, Pajović SB, Drakulić D. Comparable Toxicity of Surface-Modified TiO2 Nanoparticles: An In Vivo Experimental Study on Reproductive Toxicity in Rats. in Antioxidants. 2024;13(2):231.
doi:10.3390/antiox13020231 .

Todorović, Ana, Bobić, Katarina, Veljković, Filip, Pejić, Snežana, Glumac, Sofija, Stanković, Sanja, Milovanović, Tijana, Vukoje, Ivana, Nedeljković, Jovan, Radojević Škodrić, Sanja, Pajović, Snežana B., Drakulić, Dunja, "Comparable Toxicity of Surface-Modified TiO2 Nanoparticles: An In Vivo Experimental Study on Reproductive Toxicity in Rats" in Antioxidants, 13, no. 2 (2024):231,
https://doi.org/10.3390/antiox13020231 . .

TY  - CONF
AU  - Bobić, Katarina
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Veljković, Filip
AU  - Pajović, Snežana
AU  - Nedeljković, Jovan
AU  - Drakulić, Dunja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12810
AB  - Titanium dioxide nanoparticles (Ti02 NPs) are widely used in different cosmetic products, food additives and pharmaceuticals. Their accumulation within the body is associated with adverse health effects. Since animal studies indicate that their outcomes could be ameliorated by different modifications, we tested the impact of Ti02 NPs surface-modified with 5-aminosalicylic acid (Ti02/5-ASA NPs) in female adult Wistar rats. For the purpose of this experiment,14 days following the acute oral intake, the effects of commercially available Ti02 NPs and i02/5-ASA NPs were compared, using well defined methods, to assess the signs of acute toxicity (agitation, convulsion, and mortality) and neurological function (visual placing,
spontaneous activity, and pacing/circling) along with the levels of end products of lipid peroxidation (LPP) in eyes. Our results indicate that following the treatments, spontaneous activity was disturbed while mortality, convulsion and visual placing were unaltered. The modification decreased Ti02 NPs-induced
impairment of agitation and pacing/circling. The insight into biomolecules in the eyes, more precisely into a marker of oxidative stress (LPP), showed that it was decreased after treatment with Ti02/5-ASA NPs in comparison to Ti02 NPs, indicating that this type of modification might mitigate Ti02 NPs-induced oxidative stress. According to obtained results, even though the exact  mechanism of the actions is still unknown, the modification with 5-aminosalicylic acid alleviates toxic effects of commercially available Ti02 NPs.
PB  - Vienna : Young Scientist Association
C3  - 18th YSA PhD Symposium 2023 : Book of abstracts
T1  - Rat toxicity study of orally administrated titanium dioxide nanoparticles modified with 5-aminosalicylic acid
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12810
ER  - 

@conference{
author = "Bobić, Katarina and Todorović, Ana and Pejić, Snežana and Veljković, Filip and Pajović, Snežana and Nedeljković, Jovan and Drakulić, Dunja",
year = "2023",
abstract = "Titanium dioxide nanoparticles (Ti02 NPs) are widely used in different cosmetic products, food additives and pharmaceuticals. Their accumulation within the body is associated with adverse health effects. Since animal studies indicate that their outcomes could be ameliorated by different modifications, we tested the impact of Ti02 NPs surface-modified with 5-aminosalicylic acid (Ti02/5-ASA NPs) in female adult Wistar rats. For the purpose of this experiment,14 days following the acute oral intake, the effects of commercially available Ti02 NPs and i02/5-ASA NPs were compared, using well defined methods, to assess the signs of acute toxicity (agitation, convulsion, and mortality) and neurological function (visual placing,
spontaneous activity, and pacing/circling) along with the levels of end products of lipid peroxidation (LPP) in eyes. Our results indicate that following the treatments, spontaneous activity was disturbed while mortality, convulsion and visual placing were unaltered. The modification decreased Ti02 NPs-induced
impairment of agitation and pacing/circling. The insight into biomolecules in the eyes, more precisely into a marker of oxidative stress (LPP), showed that it was decreased after treatment with Ti02/5-ASA NPs in comparison to Ti02 NPs, indicating that this type of modification might mitigate Ti02 NPs-induced oxidative stress. According to obtained results, even though the exact  mechanism of the actions is still unknown, the modification with 5-aminosalicylic acid alleviates toxic effects of commercially available Ti02 NPs.",
publisher = "Vienna : Young Scientist Association",
journal = "18th YSA PhD Symposium 2023 : Book of abstracts",
title = "Rat toxicity study of orally administrated titanium dioxide nanoparticles modified with 5-aminosalicylic acid",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12810"
}

Bobić, K., Todorović, A., Pejić, S., Veljković, F., Pajović, S., Nedeljković, J.,& Drakulić, D.. (2023). Rat toxicity study of orally administrated titanium dioxide nanoparticles modified with 5-aminosalicylic acid. in 18th YSA PhD Symposium 2023 : Book of abstracts
Vienna : Young Scientist Association..
https://hdl.handle.net/21.15107/rcub_vinar_12810

Bobić K, Todorović A, Pejić S, Veljković F, Pajović S, Nedeljković J, Drakulić D. Rat toxicity study of orally administrated titanium dioxide nanoparticles modified with 5-aminosalicylic acid. in 18th YSA PhD Symposium 2023 : Book of abstracts. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_12810 .

Bobić, Katarina, Todorović, Ana, Pejić, Snežana, Veljković, Filip, Pajović, Snežana, Nedeljković, Jovan, Drakulić, Dunja, "Rat toxicity study of orally administrated titanium dioxide nanoparticles modified with 5-aminosalicylic acid" in 18th YSA PhD Symposium 2023 : Book of abstracts (2023),
https://hdl.handle.net/21.15107/rcub_vinar_12810 .

TY  - CONF
AU  - Spasojević-Tišma, V.
AU  - Jeremić, M.
AU  - Pejić, Snežana
AU  - Drakulić, Dunja R.
AU  - Stanković, S.
AU  - Košutić, D.
AU  - Todorović, Ana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12865
AB  - BACKGROUND-AIM Differentiated thyroid cancer (DTC) is the most rapidly diagnosed cancer worldwide and the most frequent endocrinemalignancy, comprised of differentiated subtypes, specifically papillary and follicular thyroid cancer. Thyroidectomyis currently the preferred treatment for DTC. However, adjuvant therapy in the form of radioactive iodine (131I) is oftenadministered to reduce the risk of tumor recurrence and to facilitate future cancer surveillance. As 131I treatmentadditionally might upregulate oxidative stress regularly observed in thyroid cancer patients, the aim of our study wasto investigate the alterations in the levels of glutathione (GSH), superoxide anion (O2-), nitric oxide (NO) and activityof superoxide dismutase (SOD) in blood of DTC patients treated with 131I. METHODS All investigated parameters were determined with well-established spectrophotometric methods in blood samples ofthyroidectomized DTC patients obtained 0 (before), 3, 7 and 30 days after the 131I treatment. The treatment was orallyadministered once in the dose of 3.7 GBq. RESULTS Our results showed that, compared to the values before the therapy, SOD activity was significantly increased on the3th and 30th day after the 131I treatment alongwith the levels of GSH on 7th day. Other investigated oxidative stressindicators were unchanged. According to obtained data, in the blood of thyroidectomized DTC patients during the first30 days after the therapy, increased antioxidative capacity, reflected through upregulated SOD activity and GSH levels,might be sufficient to prevent the increase of O2- and NO and, in general, suppress the oxidative stress. CONCLUSIONS Our findings provide a better insight into individual antioxidant parameters fluctuations, thus contribute to a betterunderstanding of the redox mechanisms critical for 131I treatment outcomes, since an increase in the oxidative stressin the early stages of therapy can affect later health concerns and may represent a potential risk factor for cancerprogression in DTC patients.
PB  - Berlin ; Boston : Walter de Gruyter
C3  - Clinical chemistry and laboratory medicine
T1  - Oxidative Stress in Differentiated Thyroid Cancer Patients After Radioiodine Treatment
VL  - 61
IS  - Special Supplement
SP  - S1416
EP  - S1416
DO  - 10.1515/cclm-2023-7051
ER  - 

@conference{
author = "Spasojević-Tišma, V. and Jeremić, M. and Pejić, Snežana and Drakulić, Dunja R. and Stanković, S. and Košutić, D. and Todorović, Ana",
year = "2023",
abstract = "BACKGROUND-AIM Differentiated thyroid cancer (DTC) is the most rapidly diagnosed cancer worldwide and the most frequent endocrinemalignancy, comprised of differentiated subtypes, specifically papillary and follicular thyroid cancer. Thyroidectomyis currently the preferred treatment for DTC. However, adjuvant therapy in the form of radioactive iodine (131I) is oftenadministered to reduce the risk of tumor recurrence and to facilitate future cancer surveillance. As 131I treatmentadditionally might upregulate oxidative stress regularly observed in thyroid cancer patients, the aim of our study wasto investigate the alterations in the levels of glutathione (GSH), superoxide anion (O2-), nitric oxide (NO) and activityof superoxide dismutase (SOD) in blood of DTC patients treated with 131I. METHODS All investigated parameters were determined with well-established spectrophotometric methods in blood samples ofthyroidectomized DTC patients obtained 0 (before), 3, 7 and 30 days after the 131I treatment. The treatment was orallyadministered once in the dose of 3.7 GBq. RESULTS Our results showed that, compared to the values before the therapy, SOD activity was significantly increased on the3th and 30th day after the 131I treatment alongwith the levels of GSH on 7th day. Other investigated oxidative stressindicators were unchanged. According to obtained data, in the blood of thyroidectomized DTC patients during the first30 days after the therapy, increased antioxidative capacity, reflected through upregulated SOD activity and GSH levels,might be sufficient to prevent the increase of O2- and NO and, in general, suppress the oxidative stress. CONCLUSIONS Our findings provide a better insight into individual antioxidant parameters fluctuations, thus contribute to a betterunderstanding of the redox mechanisms critical for 131I treatment outcomes, since an increase in the oxidative stressin the early stages of therapy can affect later health concerns and may represent a potential risk factor for cancerprogression in DTC patients.",
publisher = "Berlin ; Boston : Walter de Gruyter",
journal = "Clinical chemistry and laboratory medicine",
title = "Oxidative Stress in Differentiated Thyroid Cancer Patients After Radioiodine Treatment",
volume = "61",
number = "Special Supplement",
pages = "S1416-S1416",
doi = "10.1515/cclm-2023-7051"
}

Spasojević-Tišma, V., Jeremić, M., Pejić, S., Drakulić, D. R., Stanković, S., Košutić, D.,& Todorović, A.. (2023). Oxidative Stress in Differentiated Thyroid Cancer Patients After Radioiodine Treatment. in Clinical chemistry and laboratory medicine
Berlin ; Boston : Walter de Gruyter., 61(Special Supplement), S1416-S1416.
https://doi.org/10.1515/cclm-2023-7051

Spasojević-Tišma V, Jeremić M, Pejić S, Drakulić DR, Stanković S, Košutić D, Todorović A. Oxidative Stress in Differentiated Thyroid Cancer Patients After Radioiodine Treatment. in Clinical chemistry and laboratory medicine. 2023;61(Special Supplement):S1416-S1416.
doi:10.1515/cclm-2023-7051 .

Spasojević-Tišma, V., Jeremić, M., Pejić, Snežana, Drakulić, Dunja R., Stanković, S., Košutić, D., Todorović, Ana, "Oxidative Stress in Differentiated Thyroid Cancer Patients After Radioiodine Treatment" in Clinical chemistry and laboratory medicine, 61, no. Special Supplement (2023):S1416-S1416,
https://doi.org/10.1515/cclm-2023-7051 . .

TY  - CONF
AU  - Tijanić, A.
AU  - Ristić, T.
AU  - Habach, T.
AU  - Milenković, B.
AU  - Drakulić, Dunja R.
AU  - Pejić, Snežana
AU  - Stanković, S.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12858
AB  - BACKGROUND-AIM Invasive mechanical ventilation (IMV) is frequently needed intervention in patients with severe COVID19. There areattempts to identify biomarkers that could be helpful in determining the best moment to intubate these patients.It is known that cost-effective hematological indices, easily calculated from a routine complete blood count (CBC),are useful in determining patient's inflammatory response to COVID-19. The aim of this study was to examine thehematologic indices value in patients with and without the need for IMV and a control group.METHODS A total of 274 COVID-19 patients (178 males/96 females, median age 65), hospitalized in temporary COVID-19 hospitalin Clinic for Pulmonology Diseases, University Clinical Centre of Serbia in Belgrade between November 18, 2020 toJanuary 15, 2021, were stratified as IMV (Group 1) (n = 24) or did not require invasive mechanical ventilation (Group2) (n = 250). The control group was consisted of 274 healthy individuals. CBC was determined at hospital admissionusing Sysmex XN-1000 (Sysmex Corporation, Kobe, Japan). Few hematologic indices were calculated, the neutrophil-to-lymphocyte ratio (NLR), the derived NLR (d-NLR), the neutrophil-to-platelet ratio (NPR), the platelet-to-lymphocyteratio (PLR), the lymphocyte-to-monocyte ratio (LMR), and systemic immune-inflammation index (SII). RESULTS There was significant differences (p<0.001) in all hematological ratios between COVID-19 patients and the controlgroup. Patients in Group 1 had significantly higher values of NLR (7.1 (3.3-15.7) vs 4.3 (2.4-8.1), p = 0.011), dNLR (4.4(2.5-8.3) vs 2.8 (1.7-5.1), p = 0.013), NPR (0.03 (0.02-0.05) vs 0.02 (0.01-0.03), p = 0.001), compared to those in group 2.Conversely, the Group 1 had markedly decreased LMR (1.9 (1.1-3.3) vs 2.5 (1.8-3.7), p = 0.043), compared to the Group2. There was no significant difference in PLR and SII between these two groups (p >0.05). CONCLUSIONS Our study suggests that admission value of hematological indices NLR, dNLR, NPR have potential for use as auxiliaries in clinical decision-making regarding the need for IMV in COVID-19 patients.
PB  - Berlin ; Boston : Walter de Gruyter
C3  - Clinical chemistry and laboratory medicine
T1  - Initial Hematologic Indices in COVID-19 Patients With Invasive Mechanical Ventilation
VL  - 61
IS  - Special Supplement
SP  - S770
EP  - S770
DO  - 10.1515/cclm-2023-7041
ER  - 

@conference{
author = "Tijanić, A. and Ristić, T. and Habach, T. and Milenković, B. and Drakulić, Dunja R. and Pejić, Snežana and Stanković, S.",
year = "2023",
abstract = "BACKGROUND-AIM Invasive mechanical ventilation (IMV) is frequently needed intervention in patients with severe COVID19. There areattempts to identify biomarkers that could be helpful in determining the best moment to intubate these patients.It is known that cost-effective hematological indices, easily calculated from a routine complete blood count (CBC),are useful in determining patient's inflammatory response to COVID-19. The aim of this study was to examine thehematologic indices value in patients with and without the need for IMV and a control group.METHODS A total of 274 COVID-19 patients (178 males/96 females, median age 65), hospitalized in temporary COVID-19 hospitalin Clinic for Pulmonology Diseases, University Clinical Centre of Serbia in Belgrade between November 18, 2020 toJanuary 15, 2021, were stratified as IMV (Group 1) (n = 24) or did not require invasive mechanical ventilation (Group2) (n = 250). The control group was consisted of 274 healthy individuals. CBC was determined at hospital admissionusing Sysmex XN-1000 (Sysmex Corporation, Kobe, Japan). Few hematologic indices were calculated, the neutrophil-to-lymphocyte ratio (NLR), the derived NLR (d-NLR), the neutrophil-to-platelet ratio (NPR), the platelet-to-lymphocyteratio (PLR), the lymphocyte-to-monocyte ratio (LMR), and systemic immune-inflammation index (SII). RESULTS There was significant differences (p<0.001) in all hematological ratios between COVID-19 patients and the controlgroup. Patients in Group 1 had significantly higher values of NLR (7.1 (3.3-15.7) vs 4.3 (2.4-8.1), p = 0.011), dNLR (4.4(2.5-8.3) vs 2.8 (1.7-5.1), p = 0.013), NPR (0.03 (0.02-0.05) vs 0.02 (0.01-0.03), p = 0.001), compared to those in group 2.Conversely, the Group 1 had markedly decreased LMR (1.9 (1.1-3.3) vs 2.5 (1.8-3.7), p = 0.043), compared to the Group2. There was no significant difference in PLR and SII between these two groups (p >0.05). CONCLUSIONS Our study suggests that admission value of hematological indices NLR, dNLR, NPR have potential for use as auxiliaries in clinical decision-making regarding the need for IMV in COVID-19 patients.",
publisher = "Berlin ; Boston : Walter de Gruyter",
journal = "Clinical chemistry and laboratory medicine",
title = "Initial Hematologic Indices in COVID-19 Patients With Invasive Mechanical Ventilation",
volume = "61",
number = "Special Supplement",
pages = "S770-S770",
doi = "10.1515/cclm-2023-7041"
}

Tijanić, A., Ristić, T., Habach, T., Milenković, B., Drakulić, D. R., Pejić, S.,& Stanković, S.. (2023). Initial Hematologic Indices in COVID-19 Patients With Invasive Mechanical Ventilation. in Clinical chemistry and laboratory medicine
Berlin ; Boston : Walter de Gruyter., 61(Special Supplement), S770-S770.
https://doi.org/10.1515/cclm-2023-7041

Tijanić A, Ristić T, Habach T, Milenković B, Drakulić DR, Pejić S, Stanković S. Initial Hematologic Indices in COVID-19 Patients With Invasive Mechanical Ventilation. in Clinical chemistry and laboratory medicine. 2023;61(Special Supplement):S770-S770.
doi:10.1515/cclm-2023-7041 .

Tijanić, A., Ristić, T., Habach, T., Milenković, B., Drakulić, Dunja R., Pejić, Snežana, Stanković, S., "Initial Hematologic Indices in COVID-19 Patients With Invasive Mechanical Ventilation" in Clinical chemistry and laboratory medicine, 61, no. Special Supplement (2023):S770-S770,
https://doi.org/10.1515/cclm-2023-7041 . .

TY  - CONF
AU  - Bobić, Katarina
AU  - Guševac Stojanović, Ivana
AU  - Todorović, Ana
AU  - Veljković, Filip
AU  - Pejić, Snežana
AU  - Martinović, Jelena
AU  - Drakulić, Dunja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11060
AB  - Prolonged disturbance of cerebral blood flow causes metabolic insufficiency and neuronal hypofunction, for which there is still no adequate therapeutic strategy. In several animal models of neurodegenerative diseases, progesterone (P4), a potent gonadal steroid hormone, and its metabolites showed neuroprotective outcomes through reduction of oxidative stress and stabilization of membrane lipids and their downstream signalling. As P4 actions in rat striatum following permanent bilateral occlusion of both common carotid arteries are still uncertain, we investigated its capacity to reduce neuronal damage induced by permanent occlusion of both carotid arteries (2VO), focusing on several oxidative stress markers (end products of lipid peroxidation (LPP) and phosphatidylcholine (PC) to lysophosphatidylcholine (LPC) intensity ratio) in crude synaptosomal fraction. Adult male Wistar rats were divided into groups: control ‒ sham operated animals treated with vehicle (commercial flax oil, 1 mg/kg) and permanently occluded animals subjected to either vehicle (commercial flax oil, 1 mg/kg) or P4 (dissolved in commercial flax oil, 1.7 mg/kg). Animals were subcutaneously injected for 7 days and sacrificed 4 h following the last treatment. LPP levels were determined spectrophotometrically, while PC/LPC intensity ratio was estimated by mass spectrometer. Obtained results indicate that P4 treatment alleviates 2VO – induced prooxidative changes by decreasing LPP levels and elevating PC/LPC intensity ratio, and returning them closer to levels observed in controls. According to our findings, P4 treatment in cerebral hypoperfusion model, via targeting striatal cell lipid components and altering lipid profile, might be implicated in reduction of oxidative stress and promotion of protective environment.
PB  - Belgrade : Serbian Neurocardiological Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Progesterone modulates striatal lipid profile in rat cerebral hypoperfusion model
SP  - 119
EP  - 119
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11060
ER  - 

@conference{
author = "Bobić, Katarina and Guševac Stojanović, Ivana and Todorović, Ana and Veljković, Filip and Pejić, Snežana and Martinović, Jelena and Drakulić, Dunja",
year = "2023",
abstract = "Prolonged disturbance of cerebral blood flow causes metabolic insufficiency and neuronal hypofunction, for which there is still no adequate therapeutic strategy. In several animal models of neurodegenerative diseases, progesterone (P4), a potent gonadal steroid hormone, and its metabolites showed neuroprotective outcomes through reduction of oxidative stress and stabilization of membrane lipids and their downstream signalling. As P4 actions in rat striatum following permanent bilateral occlusion of both common carotid arteries are still uncertain, we investigated its capacity to reduce neuronal damage induced by permanent occlusion of both carotid arteries (2VO), focusing on several oxidative stress markers (end products of lipid peroxidation (LPP) and phosphatidylcholine (PC) to lysophosphatidylcholine (LPC) intensity ratio) in crude synaptosomal fraction. Adult male Wistar rats were divided into groups: control ‒ sham operated animals treated with vehicle (commercial flax oil, 1 mg/kg) and permanently occluded animals subjected to either vehicle (commercial flax oil, 1 mg/kg) or P4 (dissolved in commercial flax oil, 1.7 mg/kg). Animals were subcutaneously injected for 7 days and sacrificed 4 h following the last treatment. LPP levels were determined spectrophotometrically, while PC/LPC intensity ratio was estimated by mass spectrometer. Obtained results indicate that P4 treatment alleviates 2VO – induced prooxidative changes by decreasing LPP levels and elevating PC/LPC intensity ratio, and returning them closer to levels observed in controls. According to our findings, P4 treatment in cerebral hypoperfusion model, via targeting striatal cell lipid components and altering lipid profile, might be implicated in reduction of oxidative stress and promotion of protective environment.",
publisher = "Belgrade : Serbian Neurocardiological Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Progesterone modulates striatal lipid profile in rat cerebral hypoperfusion model",
pages = "119-119",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11060"
}

Bobić, K., Guševac Stojanović, I., Todorović, A., Veljković, F., Pejić, S., Martinović, J.,& Drakulić, D.. (2023). Progesterone modulates striatal lipid profile in rat cerebral hypoperfusion model. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neurocardiological Society., 119-119.
https://hdl.handle.net/21.15107/rcub_vinar_11060

Bobić K, Guševac Stojanović I, Todorović A, Veljković F, Pejić S, Martinović J, Drakulić D. Progesterone modulates striatal lipid profile in rat cerebral hypoperfusion model. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:119-119.
https://hdl.handle.net/21.15107/rcub_vinar_11060 .

Bobić, Katarina, Guševac Stojanović, Ivana, Todorović, Ana, Veljković, Filip, Pejić, Snežana, Martinović, Jelena, Drakulić, Dunja, "Progesterone modulates striatal lipid profile in rat cerebral hypoperfusion model" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):119-119,
https://hdl.handle.net/21.15107/rcub_vinar_11060 .

TY  - JOUR
AU  - Grigorov, Ilijana
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Drakulić, Dunja
AU  - Veljković, Filip
AU  - Miletić Vukajlović, Jadranka
AU  - Bobić, Katarina
AU  - Soldatović, Ivan
AU  - Đurašević, Siniša
AU  - Jasnić, Nebojša
AU  - Stanković, Sanja
AU  - Glumac, Sofija
AU  - Mihailović-Vučinić, Violeta
AU  - Milenković, Branislava
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11550
AB  - The careful monitoring of patients with mild/moderate COVID-19 is of particular importance because of the rapid progression of complications associated with COVID-19. For prognostic reasons and for the economic management of health care resources, additional biomarkers need to be identified, and their monitoring can conceivably be performed in the early stages of the disease. In this retrospective cross-sectional study, we found that serum concentrations of high-mobility group box 1 (HMGB1) and heme oxygenase-1 (HO-1), at the time of hospital admission, could be useful biomarkers for COVID-19 management. The study included 160 randomly selected recovered patients with mild to moderate COVID-19 on admission. Compared with healthy controls, serum HMGB1 and HO-1 levels increased by 487.6 pg/mL versus 43.1 pg/mL and 1497.7 pg/mL versus 756.1 pg/mL, respectively. Serum HO-1 correlated significantly with serum HMGB1, oxidative stress parameters (malondialdehyde (MDA), the phosphatidylcholine/lysophosphatidylcholine ratio (PC/LPC), the ratio of reduced and oxidative glutathione (GSH/GSSG)), and anti-inflammatory acute phase proteins (ferritin, haptoglobin). Increased heme catabolism/hemolysis were not detected. We hypothesize that the increase in HO-1 in the early phase of COVID-19 disease is likely to have a survival benefit by providing protection against oxidative stress and inflammation, whereas the level of HMGB1 increase reflects the activity of the innate immune system and represents levels within which the disease can be kept under control.
T2  - International Journal of Molecular Sciences
T1  - Serum High-Mobility Group Box 1 and Heme Oxygenase-1 as Biomarkers in COVID-19 Patients at Hospital Admission
VL  - 24
IS  - 17
SP  - 13164
DO  - 10.3390/ijms241713164
ER  - 

@article{
author = "Grigorov, Ilijana and Pejić, Snežana and Todorović, Ana and Drakulić, Dunja and Veljković, Filip and Miletić Vukajlović, Jadranka and Bobić, Katarina and Soldatović, Ivan and Đurašević, Siniša and Jasnić, Nebojša and Stanković, Sanja and Glumac, Sofija and Mihailović-Vučinić, Violeta and Milenković, Branislava",
year = "2023",
abstract = "The careful monitoring of patients with mild/moderate COVID-19 is of particular importance because of the rapid progression of complications associated with COVID-19. For prognostic reasons and for the economic management of health care resources, additional biomarkers need to be identified, and their monitoring can conceivably be performed in the early stages of the disease. In this retrospective cross-sectional study, we found that serum concentrations of high-mobility group box 1 (HMGB1) and heme oxygenase-1 (HO-1), at the time of hospital admission, could be useful biomarkers for COVID-19 management. The study included 160 randomly selected recovered patients with mild to moderate COVID-19 on admission. Compared with healthy controls, serum HMGB1 and HO-1 levels increased by 487.6 pg/mL versus 43.1 pg/mL and 1497.7 pg/mL versus 756.1 pg/mL, respectively. Serum HO-1 correlated significantly with serum HMGB1, oxidative stress parameters (malondialdehyde (MDA), the phosphatidylcholine/lysophosphatidylcholine ratio (PC/LPC), the ratio of reduced and oxidative glutathione (GSH/GSSG)), and anti-inflammatory acute phase proteins (ferritin, haptoglobin). Increased heme catabolism/hemolysis were not detected. We hypothesize that the increase in HO-1 in the early phase of COVID-19 disease is likely to have a survival benefit by providing protection against oxidative stress and inflammation, whereas the level of HMGB1 increase reflects the activity of the innate immune system and represents levels within which the disease can be kept under control.",
journal = "International Journal of Molecular Sciences",
title = "Serum High-Mobility Group Box 1 and Heme Oxygenase-1 as Biomarkers in COVID-19 Patients at Hospital Admission",
volume = "24",
number = "17",
pages = "13164",
doi = "10.3390/ijms241713164"
}

Grigorov, I., Pejić, S., Todorović, A., Drakulić, D., Veljković, F., Miletić Vukajlović, J., Bobić, K., Soldatović, I., Đurašević, S., Jasnić, N., Stanković, S., Glumac, S., Mihailović-Vučinić, V.,& Milenković, B.. (2023). Serum High-Mobility Group Box 1 and Heme Oxygenase-1 as Biomarkers in COVID-19 Patients at Hospital Admission. in International Journal of Molecular Sciences, 24(17), 13164.
https://doi.org/10.3390/ijms241713164

Grigorov I, Pejić S, Todorović A, Drakulić D, Veljković F, Miletić Vukajlović J, Bobić K, Soldatović I, Đurašević S, Jasnić N, Stanković S, Glumac S, Mihailović-Vučinić V, Milenković B. Serum High-Mobility Group Box 1 and Heme Oxygenase-1 as Biomarkers in COVID-19 Patients at Hospital Admission. in International Journal of Molecular Sciences. 2023;24(17):13164.
doi:10.3390/ijms241713164 .

Grigorov, Ilijana, Pejić, Snežana, Todorović, Ana, Drakulić, Dunja, Veljković, Filip, Miletić Vukajlović, Jadranka, Bobić, Katarina, Soldatović, Ivan, Đurašević, Siniša, Jasnić, Nebojša, Stanković, Sanja, Glumac, Sofija, Mihailović-Vučinić, Violeta, Milenković, Branislava, "Serum High-Mobility Group Box 1 and Heme Oxygenase-1 as Biomarkers in COVID-19 Patients at Hospital Admission" in International Journal of Molecular Sciences, 24, no. 17 (2023):13164,
https://doi.org/10.3390/ijms241713164 . .

TY  - CONF
AU  - Veljković, Filip
AU  - Drakulić, Dunja
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Bobić, Katarina
AU  - Stajčić, Ivana
AU  - Pajović, Snežana
AU  - Veličković, Suzana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12663
AB  - It is known that the “superalkali” cluster Li2F can significantly improve the hydrogen storage capabilities in carbon materials; boron compounds play a similar role. However, the possibility of interactions of lithium fluoride clusters with boron and hydrogen has not been investigated so far. In this work, a laser desorption/ionization mass spectrometry (LDI MS) was used for this purpose. Preliminary results showed that the interaction between the “superalkali” Li3F cluster, boron and six hydrogen atoms is possible; the ion Li3FBH6+ was detected. Non-stoichiometric clusters of lithium fluoride can also combine with boron and hydrogen in the following manner: Li5F2BH5+ Li5F3BH2+ Li4F5BH4+ Li6F2B6H+, and Li8F6H+.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - Investigation of the possibility of interaction between lithium fluoride clusters and boron using LDI MS
SP  - 176
EP  - 179
DO  - 10.46793/ICCBI23.176V
ER  - 

@conference{
author = "Veljković, Filip and Drakulić, Dunja and Todorović, Ana and Pejić, Snežana and Bobić, Katarina and Stajčić, Ivana and Pajović, Snežana and Veličković, Suzana",
year = "2023",
abstract = "It is known that the “superalkali” cluster Li2F can significantly improve the hydrogen storage capabilities in carbon materials; boron compounds play a similar role. However, the possibility of interactions of lithium fluoride clusters with boron and hydrogen has not been investigated so far. In this work, a laser desorption/ionization mass spectrometry (LDI MS) was used for this purpose. Preliminary results showed that the interaction between the “superalkali” Li3F cluster, boron and six hydrogen atoms is possible; the ion Li3FBH6+ was detected. Non-stoichiometric clusters of lithium fluoride can also combine with boron and hydrogen in the following manner: Li5F2BH5+ Li5F3BH2+ Li4F5BH4+ Li6F2B6H+, and Li8F6H+.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "Investigation of the possibility of interaction between lithium fluoride clusters and boron using LDI MS",
pages = "176-179",
doi = "10.46793/ICCBI23.176V"
}

Veljković, F., Drakulić, D., Todorović, A., Pejić, S., Bobić, K., Stajčić, I., Pajović, S.,& Veličković, S.. (2023). Investigation of the possibility of interaction between lithium fluoride clusters and boron using LDI MS. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 176-179.
https://doi.org/10.46793/ICCBI23.176V

Veljković F, Drakulić D, Todorović A, Pejić S, Bobić K, Stajčić I, Pajović S, Veličković S. Investigation of the possibility of interaction between lithium fluoride clusters and boron using LDI MS. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:176-179.
doi:10.46793/ICCBI23.176V .

Veljković, Filip, Drakulić, Dunja, Todorović, Ana, Pejić, Snežana, Bobić, Katarina, Stajčić, Ivana, Pajović, Snežana, Veličković, Suzana, "Investigation of the possibility of interaction between lithium fluoride clusters and boron using LDI MS" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):176-179,
https://doi.org/10.46793/ICCBI23.176V . .

TY  - CONF
AU  - Guševac Stojanović, Ivana
AU  - Dragić, Milorad
AU  - Zarić Kontić, Marina
AU  - Martinović, Jelena
AU  - Mitrović, Nataša
AU  - Stojanović, Zoran
AU  - Veljković, Filip
AU  - Martinović, D.
AU  - Grković, Ivana
AU  - Drakulić, Dunja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11058
AB  - Cerebral hypoperfusion (CH) is recognised as a contributor to various impairments characteristic for elderly population and patients with vascular dementia and Alzheimer’s disease. CH-induced brain damage is linked with oxidative stress in the cells that can cause DNA fragmentation and cell death, reflected through a change in cells’ morphology. Our study investigated the beneficial effects of progesterone (P4), a hormone with neuroprotective properties, against CH-induced oxidative stress and neurodegenerative pathologies in rat prefrontal cortex (PFC). For the purpose of the experiment, adult male Wistar rats were dived into groups: (I) animals subjected to permanent bilateral occlusion of common carotid arteries (2VO) treated with vehicle (commercial flax oil, 1 mg/kg/day), (II) animals subjected to 2VO treated with P4 dissolved in vehicle (1.7 mg/kg/day) and (III) animals subjected to sham operation treated with vehicle. Animals were sacrificed after 7 subcutaneous treatments. Levels of pro-/antioxidant balance (PAB) and DNA fragmentation along with cell morphology were estimated by well-defined methods. The results revealed that P4 administration moderated CH-induced impairments in PFC, not only by decreasing PAB level and diminishing DNA fragmentation, but also preserving the cell morphology reflected through clearly defined cell bodies, with round nuclei, prominent nucleolus and visible Nissl bodies in layer III. Obtained results point out that P4 is able to attenuate CH-induced pro-oxidant state and subsequent changes in PFC. This hormone holds promise as an effective agent for the CH treatment, still, its specific actions remain to be discovered.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model
SP  - 117
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11058
ER  - 

@conference{
author = "Guševac Stojanović, Ivana and Dragić, Milorad and Zarić Kontić, Marina and Martinović, Jelena and Mitrović, Nataša and Stojanović, Zoran and Veljković, Filip and Martinović, D. and Grković, Ivana and Drakulić, Dunja",
year = "2023",
abstract = "Cerebral hypoperfusion (CH) is recognised as a contributor to various impairments characteristic for elderly population and patients with vascular dementia and Alzheimer’s disease. CH-induced brain damage is linked with oxidative stress in the cells that can cause DNA fragmentation and cell death, reflected through a change in cells’ morphology. Our study investigated the beneficial effects of progesterone (P4), a hormone with neuroprotective properties, against CH-induced oxidative stress and neurodegenerative pathologies in rat prefrontal cortex (PFC). For the purpose of the experiment, adult male Wistar rats were dived into groups: (I) animals subjected to permanent bilateral occlusion of common carotid arteries (2VO) treated with vehicle (commercial flax oil, 1 mg/kg/day), (II) animals subjected to 2VO treated with P4 dissolved in vehicle (1.7 mg/kg/day) and (III) animals subjected to sham operation treated with vehicle. Animals were sacrificed after 7 subcutaneous treatments. Levels of pro-/antioxidant balance (PAB) and DNA fragmentation along with cell morphology were estimated by well-defined methods. The results revealed that P4 administration moderated CH-induced impairments in PFC, not only by decreasing PAB level and diminishing DNA fragmentation, but also preserving the cell morphology reflected through clearly defined cell bodies, with round nuclei, prominent nucleolus and visible Nissl bodies in layer III. Obtained results point out that P4 is able to attenuate CH-induced pro-oxidant state and subsequent changes in PFC. This hormone holds promise as an effective agent for the CH treatment, still, its specific actions remain to be discovered.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model",
pages = "117",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11058"
}

Guševac Stojanović, I., Dragić, M., Zarić Kontić, M., Martinović, J., Mitrović, N., Stojanović, Z., Veljković, F., Martinović, D., Grković, I.,& Drakulić, D.. (2023). Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 117.
https://hdl.handle.net/21.15107/rcub_vinar_11058

Guševac Stojanović I, Dragić M, Zarić Kontić M, Martinović J, Mitrović N, Stojanović Z, Veljković F, Martinović D, Grković I, Drakulić D. Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:117.
https://hdl.handle.net/21.15107/rcub_vinar_11058 .

Guševac Stojanović, Ivana, Dragić, Milorad, Zarić Kontić, Marina, Martinović, Jelena, Mitrović, Nataša, Stojanović, Zoran, Veljković, Filip, Martinović, D., Grković, Ivana, Drakulić, Dunja, "Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):117,
https://hdl.handle.net/21.15107/rcub_vinar_11058 .

TY  - CONF
AU  - Martinović, Jelena
AU  - Zarić Kontić, Marina
AU  - Guševac Stojanović, Ivana
AU  - Mitrović, Nataša
AU  - Grković, Ivana
AU  - Stojanović, Zoran
AU  - Drakulić, Dunja
AU  - Samardžić, Janko
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11047
AB  - Zaleplon, a member of Z-drugs, is a pyrazolopyrimidine hypnotic with sedative, anxiolytic, anticonvulsant and muscle relaxant properties. Zaleplon is approved for the short-term management of insomnia since acting as positive γ-aminobutyric acid (GABA) receptor allosteric modulator increases efficacy of inhibition on brain excitability. Importantly, for the proper functioning of the brain a balance between inhibitory (i.e., GABAergic) and excitatory (i.e., glutamatergic) system must be accomplished. This may be fulfilled by control of presynaptic elements (synthesis or degradation of glutamate and GABA neurotransmitters, their compartmentation, releasing and recycling) and regulation of expression and function of glutamate and GABA receptors. Hence, we aimed to investigate effects of prolonged zaleplon treatment on the expression of proteins involved in the gabaergic and glutamatergic signalization in the hippocampus of adult male Wistar rats. Five-day intraperitoneal administration increased level of components of GABAergic signalization (glutamate decarboxylase 67-GAD67, vesicular GABA transporter-VGAT and α1 subunit of GABA receptor-GABAAα1). This was accompanied by increased level of glutamatergic components (vesicular glutamate transporter 1-vGlut1 and subunits of glutamate N-Methyl-d-aspartate receptor-NMDAR, namely NR1, NR2A, NR2B), which clearly indicate maintenance of balance between main inhibitory and excitatory neurotransmitters. Given the importance of equilibrium of these systems for neuronal excitability, synaptic plasticity and cognitive functions, as well as its involvement in the mood, feeding behavior, reproductive functions, pain sensitivity, aging, etc., the current and prospective pharmaceuticals increasingly rely on GABA/glutamate balance
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats
SP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11047
ER  - 

@conference{
author = "Martinović, Jelena and Zarić Kontić, Marina and Guševac Stojanović, Ivana and Mitrović, Nataša and Grković, Ivana and Stojanović, Zoran and Drakulić, Dunja and Samardžić, Janko",
year = "2023",
abstract = "Zaleplon, a member of Z-drugs, is a pyrazolopyrimidine hypnotic with sedative, anxiolytic, anticonvulsant and muscle relaxant properties. Zaleplon is approved for the short-term management of insomnia since acting as positive γ-aminobutyric acid (GABA) receptor allosteric modulator increases efficacy of inhibition on brain excitability. Importantly, for the proper functioning of the brain a balance between inhibitory (i.e., GABAergic) and excitatory (i.e., glutamatergic) system must be accomplished. This may be fulfilled by control of presynaptic elements (synthesis or degradation of glutamate and GABA neurotransmitters, their compartmentation, releasing and recycling) and regulation of expression and function of glutamate and GABA receptors. Hence, we aimed to investigate effects of prolonged zaleplon treatment on the expression of proteins involved in the gabaergic and glutamatergic signalization in the hippocampus of adult male Wistar rats. Five-day intraperitoneal administration increased level of components of GABAergic signalization (glutamate decarboxylase 67-GAD67, vesicular GABA transporter-VGAT and α1 subunit of GABA receptor-GABAAα1). This was accompanied by increased level of glutamatergic components (vesicular glutamate transporter 1-vGlut1 and subunits of glutamate N-Methyl-d-aspartate receptor-NMDAR, namely NR1, NR2A, NR2B), which clearly indicate maintenance of balance between main inhibitory and excitatory neurotransmitters. Given the importance of equilibrium of these systems for neuronal excitability, synaptic plasticity and cognitive functions, as well as its involvement in the mood, feeding behavior, reproductive functions, pain sensitivity, aging, etc., the current and prospective pharmaceuticals increasingly rely on GABA/glutamate balance",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats",
pages = "59",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11047"
}

Martinović, J., Zarić Kontić, M., Guševac Stojanović, I., Mitrović, N., Grković, I., Stojanović, Z., Drakulić, D.,& Samardžić, J.. (2023). Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 59.
https://hdl.handle.net/21.15107/rcub_vinar_11047

Martinović J, Zarić Kontić M, Guševac Stojanović I, Mitrović N, Grković I, Stojanović Z, Drakulić D, Samardžić J. Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:59.
https://hdl.handle.net/21.15107/rcub_vinar_11047 .

Martinović, Jelena, Zarić Kontić, Marina, Guševac Stojanović, Ivana, Mitrović, Nataša, Grković, Ivana, Stojanović, Zoran, Drakulić, Dunja, Samardžić, Janko, "Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):59,
https://hdl.handle.net/21.15107/rcub_vinar_11047 .

TY  - CONF
AU  - Bobić, Katarina
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Veljković, Filip
AU  - Veličković, Suzana
AU  - Pajović, Snežana B.
AU  - Drakulić, Dunja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12389
AB  - Titanium dioxide nanoparticles (TiO2NPs) are widely used in pharmaceutics, food products and cosmetics. Besides numerous beneficial outcomes, they also induce toxic effects in different organs, including the eyes. The recent findings report that some surface modifications can attenuate TiO2NPs toxicity. Thus, the aim of this study was to compare the effects of acute oral treatment with commercially available, bare TiO2NPs and TiO2NPs surface modified with salicylic acid (SA-TiO2NPs) using adult Wistar rats as model organisms. Control animals were gastrically intubated with vehicle (0.01 M HCl), while others were treated with either TiO2NPs or SA-TiO2NPs (1000mg/kg dissolved in vehicle). Afterward, the mortality rate as the acute toxicity parameter and parameters of neurological function were assessed. Animals were sacrificed on the 14th day following treatment. In the eye crude synaptosomal fraction, the effects of treatments on oxidative stress markers such as prooxidant/antioxidant balance (PAB) and lipid peroxidation (LPO) were compared. According to the obtained results, the mortality was 0 in all experimental groups. TiO2NPs and SA-TiO2NPs treatments displayed mild effects on spontaneous activity and pacing and had no impact on the visual placing reflex. Moreover, TiO2NPs and SA-TiO2NPs exposure significantly elevated PAB levels, when compared to controls. Animals treated with SA-TiO2NPs had similar LPO levels as controls, while in TiO2NPs group LPO levels were significantly increased. The presented data showed that both examined nanoparticles exerted similar outcomes referring to most of the investigated parameters, however, surface binding of SA decreased the level of LPO and thus, mitigated some of TiO2NPs toxicity in the eyes.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - TiO2 nanoparticles and TiO2 nanoparticles surface modified with salicylic acid affect neurological functions and oxidative stress markers in the eyes of adult rats
SP  - 499
EP  - 502
DO  - 10.46793/ICCBI23.499B
ER  - 

@conference{
author = "Bobić, Katarina and Todorović, Ana and Pejić, Snežana and Veljković, Filip and Veličković, Suzana and Pajović, Snežana B. and Drakulić, Dunja",
year = "2023",
abstract = "Titanium dioxide nanoparticles (TiO2NPs) are widely used in pharmaceutics, food products and cosmetics. Besides numerous beneficial outcomes, they also induce toxic effects in different organs, including the eyes. The recent findings report that some surface modifications can attenuate TiO2NPs toxicity. Thus, the aim of this study was to compare the effects of acute oral treatment with commercially available, bare TiO2NPs and TiO2NPs surface modified with salicylic acid (SA-TiO2NPs) using adult Wistar rats as model organisms. Control animals were gastrically intubated with vehicle (0.01 M HCl), while others were treated with either TiO2NPs or SA-TiO2NPs (1000mg/kg dissolved in vehicle). Afterward, the mortality rate as the acute toxicity parameter and parameters of neurological function were assessed. Animals were sacrificed on the 14th day following treatment. In the eye crude synaptosomal fraction, the effects of treatments on oxidative stress markers such as prooxidant/antioxidant balance (PAB) and lipid peroxidation (LPO) were compared. According to the obtained results, the mortality was 0 in all experimental groups. TiO2NPs and SA-TiO2NPs treatments displayed mild effects on spontaneous activity and pacing and had no impact on the visual placing reflex. Moreover, TiO2NPs and SA-TiO2NPs exposure significantly elevated PAB levels, when compared to controls. Animals treated with SA-TiO2NPs had similar LPO levels as controls, while in TiO2NPs group LPO levels were significantly increased. The presented data showed that both examined nanoparticles exerted similar outcomes referring to most of the investigated parameters, however, surface binding of SA decreased the level of LPO and thus, mitigated some of TiO2NPs toxicity in the eyes.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "TiO2 nanoparticles and TiO2 nanoparticles surface modified with salicylic acid affect neurological functions and oxidative stress markers in the eyes of adult rats",
pages = "499-502",
doi = "10.46793/ICCBI23.499B"
}

Bobić, K., Todorović, A., Pejić, S., Veljković, F., Veličković, S., Pajović, S. B.,& Drakulić, D.. (2023). TiO2 nanoparticles and TiO2 nanoparticles surface modified with salicylic acid affect neurological functions and oxidative stress markers in the eyes of adult rats. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 499-502.
https://doi.org/10.46793/ICCBI23.499B

Bobić K, Todorović A, Pejić S, Veljković F, Veličković S, Pajović SB, Drakulić D. TiO2 nanoparticles and TiO2 nanoparticles surface modified with salicylic acid affect neurological functions and oxidative stress markers in the eyes of adult rats. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:499-502.
doi:10.46793/ICCBI23.499B .

Bobić, Katarina, Todorović, Ana, Pejić, Snežana, Veljković, Filip, Veličković, Suzana, Pajović, Snežana B., Drakulić, Dunja, "TiO2 nanoparticles and TiO2 nanoparticles surface modified with salicylic acid affect neurological functions and oxidative stress markers in the eyes of adult rats" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):499-502,
https://doi.org/10.46793/ICCBI23.499B . .

TY  - CONF
AU  - Valenta Šobot, Ana
AU  - Drakulić, Dunja
AU  - Kokunešoski, Maja
AU  - Momić, Tatjana
AU  - Filipović Tričković, Jelena G.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11410
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book
T1  - Loganic acid induces apoptosis in human peripheral blood mononuclear cells
T1  - Loganska kiselina indukuje apoptozu u mononuklearnim ćelijama periferne krvi čoveka
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11410
ER  - 

@conference{
author = "Valenta Šobot, Ana and Drakulić, Dunja and Kokunešoski, Maja and Momić, Tatjana and Filipović Tričković, Jelena G.",
year = "2023",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book",
title = "Loganic acid induces apoptosis in human peripheral blood mononuclear cells, Loganska kiselina indukuje apoptozu u mononuklearnim ćelijama periferne krvi čoveka",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11410"
}

Valenta Šobot, A., Drakulić, D., Kokunešoski, M., Momić, T.,& Filipović Tričković, J. G.. (2023). Loganic acid induces apoptosis in human peripheral blood mononuclear cells. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book.
https://hdl.handle.net/21.15107/rcub_vinar_11410

Valenta Šobot A, Drakulić D, Kokunešoski M, Momić T, Filipović Tričković JG. Loganic acid induces apoptosis in human peripheral blood mononuclear cells. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_11410 .

Valenta Šobot, Ana, Drakulić, Dunja, Kokunešoski, Maja, Momić, Tatjana, Filipović Tričković, Jelena G., "Loganic acid induces apoptosis in human peripheral blood mononuclear cells" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book (2023),
https://hdl.handle.net/21.15107/rcub_vinar_11410 .

TY  - JOUR
AU  - Martinović, Jelena
AU  - Zarić Kontić, Marina
AU  - Dragić, Milorad
AU  - Todorović, Ana
AU  - Guševac Stojanović, Ivana
AU  - Mitrović, Nataša
AU  - Grković, Ivana
AU  - Drakulić, Dunja R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10461
AB  - D-galactose (d-gal) is broadly used in animal aging studies as its chronic administration mimics learning and memory impairments related to aging in humans. However, within the few studies that utilize chronic oral d-gal intake, none of them is focused on alteration in synaptic structure and function. We examined the effects of 6-weeks oral d-gal intake (200 mg/kg and 500 mg/kg, dissolved in tap water) on age-related changes, with emphasis on the prefrontal cortex (PFC) and hippocampus (HIP) of adult male Wistar rats. Memory assessment was followed by histological examination of the PFC and HIP (Nissl staining and Iba-1 immunostaining), while in crude synaptosomal fractions the state of oxidative stress and the expression of proteins involved in glutamatergic signaling was determined. Although applied dosages compromised memory, alterations such as impaired sensory-motor function and aberrant morphology were not detected. In the PFC, analysis of microglia revealed reduction of branching pattern following d-gal intake, in parallel with increased oxidative damage of proteins, lipids and disturbed pro-oxidant antioxidant balance. These changes in the PFC were further accompanied with decreased levels of vesicular glutamate transporter 1, syntaxin-1 and NMDA receptor 2B subunit in both treated groups. Simultaneously, the increased hippocampal oxidative damage of lipids was detected. Results indicate successful provocation of age-related changes following oral d-gal intake, and suggest greater sensitivity of the PFC to d-gal treatment than HIP.
T2  - Behavioural Brain Research
T1  - Chronic oral d-galactose intake provokes age-related changes in the rat prefrontal cortex
VL  - 436
SP  - 114072
DO  - 10.1016/j.bbr.2022.114072
ER  - 

@article{
author = "Martinović, Jelena and Zarić Kontić, Marina and Dragić, Milorad and Todorović, Ana and Guševac Stojanović, Ivana and Mitrović, Nataša and Grković, Ivana and Drakulić, Dunja R.",
year = "2023",
abstract = "D-galactose (d-gal) is broadly used in animal aging studies as its chronic administration mimics learning and memory impairments related to aging in humans. However, within the few studies that utilize chronic oral d-gal intake, none of them is focused on alteration in synaptic structure and function. We examined the effects of 6-weeks oral d-gal intake (200 mg/kg and 500 mg/kg, dissolved in tap water) on age-related changes, with emphasis on the prefrontal cortex (PFC) and hippocampus (HIP) of adult male Wistar rats. Memory assessment was followed by histological examination of the PFC and HIP (Nissl staining and Iba-1 immunostaining), while in crude synaptosomal fractions the state of oxidative stress and the expression of proteins involved in glutamatergic signaling was determined. Although applied dosages compromised memory, alterations such as impaired sensory-motor function and aberrant morphology were not detected. In the PFC, analysis of microglia revealed reduction of branching pattern following d-gal intake, in parallel with increased oxidative damage of proteins, lipids and disturbed pro-oxidant antioxidant balance. These changes in the PFC were further accompanied with decreased levels of vesicular glutamate transporter 1, syntaxin-1 and NMDA receptor 2B subunit in both treated groups. Simultaneously, the increased hippocampal oxidative damage of lipids was detected. Results indicate successful provocation of age-related changes following oral d-gal intake, and suggest greater sensitivity of the PFC to d-gal treatment than HIP.",
journal = "Behavioural Brain Research",
title = "Chronic oral d-galactose intake provokes age-related changes in the rat prefrontal cortex",
volume = "436",
pages = "114072",
doi = "10.1016/j.bbr.2022.114072"
}

Martinović, J., Zarić Kontić, M., Dragić, M., Todorović, A., Guševac Stojanović, I., Mitrović, N., Grković, I.,& Drakulić, D. R.. (2023). Chronic oral d-galactose intake provokes age-related changes in the rat prefrontal cortex. in Behavioural Brain Research, 436, 114072.
https://doi.org/10.1016/j.bbr.2022.114072

Martinović J, Zarić Kontić M, Dragić M, Todorović A, Guševac Stojanović I, Mitrović N, Grković I, Drakulić DR. Chronic oral d-galactose intake provokes age-related changes in the rat prefrontal cortex. in Behavioural Brain Research. 2023;436:114072.
doi:10.1016/j.bbr.2022.114072 .

Martinović, Jelena, Zarić Kontić, Marina, Dragić, Milorad, Todorović, Ana, Guševac Stojanović, Ivana, Mitrović, Nataša, Grković, Ivana, Drakulić, Dunja R., "Chronic oral d-galactose intake provokes age-related changes in the rat prefrontal cortex" in Behavioural Brain Research, 436 (2023):114072,
https://doi.org/10.1016/j.bbr.2022.114072 . .

TY  - CONF
AU  - Bobić, Katarina
AU  - Milovanović, Tijana
AU  - Todorović, Ana
AU  - Drakulić, Dunja
AU  - Pejić, Snežana
AU  - Veljković, Filip
AU  - Pajović, Snežana
AU  - Nedeljković, Jovan
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12662
AB  - Široka primena nanočestica titanijum-dioksida (TiO2 NČ) u medicini, farmaceutskoj, prehrambenoj i hemijskoj industriji,1 čini TiO2 NČ visoko zastupljenim u životnoj sredini i svim sferama čovekovog života. Negativne efekte ostvaruju u mozgu, s obzirom da lako prolaze krvno-moždanu barijeru, akumuliraju se u različitim regionima mozga i indukuju oksidativni stres tokom kog se oštećuju svi biomolekuli, uključujući i lipide.2 Literaturni podaci ukazuju da različite modifikacije mogu ublažiti uočene efekte, stoga je cilj ovog rada bio da se uporedi odgovor odraslih Wistar pacova 14 dana nakon jednokratnog oralnog tretmana sa komercijalno dostupnim TiO2 NČ i TiO2 NČ površinski modifikovanim salicilnom (TiO2/SK NČ) odnosno 5- aminosalicilnom kiselinom (TiO2/5-ASK NČ). Odgovarajućim testovima praćene su promene parametara senzorno-motorne funkcije (SMF), kao pokazatelja neurološkog deficita, dok je oksidativni stres praćen promenom nivoa lipidne peroksidacije (LPO) u ukupnom ćelijskom ekstraktu moždanog tkiva. Dobijeni rezultati ukazuju da TiO2/SK NČ ispoljavaju slične efekte kao TiO2 NČ, povećavajući nivo LPO koji može biti uzrok narušene SMF. Sa druge strane, smanjen stepen neurološkog deficita i nivoa oksidativnog stresa nakon TiO2/5- ASK NČ tretmana, mogu se povezati sa povećanjem aktivnosti komponenti antioksidativne zaštite. Stoga, može se pretpostaviti da površinska modifikacija TiO2 NČ sa 5-ASK ima sposobnost ublažavanja štetnih efekata komercijalno dostupnih TiO2 NČ.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka
T1  - Površinska modifikacija nanočestica titanijum dioksida aminosalicilnom kiselinom ublažava neurološki deficit i lipidnu peroksidaciju u mozgu Wistar pacova
SP  - 311
EP  - 311
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12662
ER  - 

@conference{
author = "Bobić, Katarina and Milovanović, Tijana and Todorović, Ana and Drakulić, Dunja and Pejić, Snežana and Veljković, Filip and Pajović, Snežana and Nedeljković, Jovan",
year = "2022",
abstract = "Široka primena nanočestica titanijum-dioksida (TiO2 NČ) u medicini, farmaceutskoj, prehrambenoj i hemijskoj industriji,1 čini TiO2 NČ visoko zastupljenim u životnoj sredini i svim sferama čovekovog života. Negativne efekte ostvaruju u mozgu, s obzirom da lako prolaze krvno-moždanu barijeru, akumuliraju se u različitim regionima mozga i indukuju oksidativni stres tokom kog se oštećuju svi biomolekuli, uključujući i lipide.2 Literaturni podaci ukazuju da različite modifikacije mogu ublažiti uočene efekte, stoga je cilj ovog rada bio da se uporedi odgovor odraslih Wistar pacova 14 dana nakon jednokratnog oralnog tretmana sa komercijalno dostupnim TiO2 NČ i TiO2 NČ površinski modifikovanim salicilnom (TiO2/SK NČ) odnosno 5- aminosalicilnom kiselinom (TiO2/5-ASK NČ). Odgovarajućim testovima praćene su promene parametara senzorno-motorne funkcije (SMF), kao pokazatelja neurološkog deficita, dok je oksidativni stres praćen promenom nivoa lipidne peroksidacije (LPO) u ukupnom ćelijskom ekstraktu moždanog tkiva. Dobijeni rezultati ukazuju da TiO2/SK NČ ispoljavaju slične efekte kao TiO2 NČ, povećavajući nivo LPO koji može biti uzrok narušene SMF. Sa druge strane, smanjen stepen neurološkog deficita i nivoa oksidativnog stresa nakon TiO2/5- ASK NČ tretmana, mogu se povezati sa povećanjem aktivnosti komponenti antioksidativne zaštite. Stoga, može se pretpostaviti da površinska modifikacija TiO2 NČ sa 5-ASK ima sposobnost ublažavanja štetnih efekata komercijalno dostupnih TiO2 NČ.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka",
title = "Površinska modifikacija nanočestica titanijum dioksida aminosalicilnom kiselinom ublažava neurološki deficit i lipidnu peroksidaciju u mozgu Wistar pacova",
pages = "311-311",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12662"
}

Bobić, K., Milovanović, T., Todorović, A., Drakulić, D., Pejić, S., Veljković, F., Pajović, S.,& Nedeljković, J.. (2022). Površinska modifikacija nanočestica titanijum dioksida aminosalicilnom kiselinom ublažava neurološki deficit i lipidnu peroksidaciju u mozgu Wistar pacova. in Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka
Beograd : Srpsko biološko društvo., 311-311.
https://hdl.handle.net/21.15107/rcub_vinar_12662

Bobić K, Milovanović T, Todorović A, Drakulić D, Pejić S, Veljković F, Pajović S, Nedeljković J. Površinska modifikacija nanočestica titanijum dioksida aminosalicilnom kiselinom ublažava neurološki deficit i lipidnu peroksidaciju u mozgu Wistar pacova. in Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka. 2022;:311-311.
https://hdl.handle.net/21.15107/rcub_vinar_12662 .

Bobić, Katarina, Milovanović, Tijana, Todorović, Ana, Drakulić, Dunja, Pejić, Snežana, Veljković, Filip, Pajović, Snežana, Nedeljković, Jovan, "Površinska modifikacija nanočestica titanijum dioksida aminosalicilnom kiselinom ublažava neurološki deficit i lipidnu peroksidaciju u mozgu Wistar pacova" in Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka (2022):311-311,
https://hdl.handle.net/21.15107/rcub_vinar_12662 .

TY  - CONF
AU  - Bobić, Katarina
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Veljković, Filip
AU  - Pajović, Snežana
AU  - Nedeljković, Jovan
AU  - Drakulić, Dunja
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12741
AB  - Titanium dioxide nanoparticles (TiO2 NPs) are widely used in many daily necessities, including medicine, food/ beverages, and cosmetics. Besides the numerous benefits of TiO2 NPs utilization, their use raises public concerns since humans are exposed to their toxicity. As different alterations could moderate the observed side effects of commercially available bare TiO2 NPs, we investigated whether TiO2/SA NPs (TiO2 NPs surface‑modified with salicylic acid (SA)) impact the bare TiO2 NPs-induced sensory-motor impairments and eye level of 4-hydroxynonenal (HNE) as the final product of lipid peroxidation and indicator of oxidative stress. Our results demonstrate that 14 days following single oral treatment both investigated TiO2 NPs in adult Wistar rats affected neurological functions and promoted oxidative stress to different extents. Namely, the SA modification alleviated TiO2 NPs-induced asymmetry in four limb movement, while the hind limb clasping reflex and spontaneous activity scores were similar between TiO2 groups. In the eye crude membrane fraction of TiO2/SA NPs-treated rats in comparison to animals treated with bare TiO2 NPs, the HNE level was downregulated. Although SA exhibits the ability to reduce some TiO2 NPs toxicity, due to still unknown mechanism of TiO2 and TiO2/SA NPs actions, further studies are required to verify herein presented findings
PB  - Poland : Nencki Institute of experimental biology
C3  - Acta Neurobiologiae experimentalis : NEURONUS 2022 : 12th Neuronus 2022 Neuroscience Forum : Abstracts
T1  - Surface modification of TiO2 nanoparticles moderates neurological impairments and upregulated oxidative stress indicator in eyes of adult Wistar rats
VL  - 82
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12741
ER  - 

@conference{
author = "Bobić, Katarina and Todorović, Ana and Pejić, Snežana and Veljković, Filip and Pajović, Snežana and Nedeljković, Jovan and Drakulić, Dunja",
year = "2022",
abstract = "Titanium dioxide nanoparticles (TiO2 NPs) are widely used in many daily necessities, including medicine, food/ beverages, and cosmetics. Besides the numerous benefits of TiO2 NPs utilization, their use raises public concerns since humans are exposed to their toxicity. As different alterations could moderate the observed side effects of commercially available bare TiO2 NPs, we investigated whether TiO2/SA NPs (TiO2 NPs surface‑modified with salicylic acid (SA)) impact the bare TiO2 NPs-induced sensory-motor impairments and eye level of 4-hydroxynonenal (HNE) as the final product of lipid peroxidation and indicator of oxidative stress. Our results demonstrate that 14 days following single oral treatment both investigated TiO2 NPs in adult Wistar rats affected neurological functions and promoted oxidative stress to different extents. Namely, the SA modification alleviated TiO2 NPs-induced asymmetry in four limb movement, while the hind limb clasping reflex and spontaneous activity scores were similar between TiO2 groups. In the eye crude membrane fraction of TiO2/SA NPs-treated rats in comparison to animals treated with bare TiO2 NPs, the HNE level was downregulated. Although SA exhibits the ability to reduce some TiO2 NPs toxicity, due to still unknown mechanism of TiO2 and TiO2/SA NPs actions, further studies are required to verify herein presented findings",
publisher = "Poland : Nencki Institute of experimental biology",
journal = "Acta Neurobiologiae experimentalis : NEURONUS 2022 : 12th Neuronus 2022 Neuroscience Forum : Abstracts",
title = "Surface modification of TiO2 nanoparticles moderates neurological impairments and upregulated oxidative stress indicator in eyes of adult Wistar rats",
volume = "82",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12741"
}

Bobić, K., Todorović, A., Pejić, S., Veljković, F., Pajović, S., Nedeljković, J.,& Drakulić, D.. (2022). Surface modification of TiO2 nanoparticles moderates neurological impairments and upregulated oxidative stress indicator in eyes of adult Wistar rats. in Acta Neurobiologiae experimentalis : NEURONUS 2022 : 12th Neuronus 2022 Neuroscience Forum : Abstracts
Poland : Nencki Institute of experimental biology., 82.
https://hdl.handle.net/21.15107/rcub_vinar_12741

Bobić K, Todorović A, Pejić S, Veljković F, Pajović S, Nedeljković J, Drakulić D. Surface modification of TiO2 nanoparticles moderates neurological impairments and upregulated oxidative stress indicator in eyes of adult Wistar rats. in Acta Neurobiologiae experimentalis : NEURONUS 2022 : 12th Neuronus 2022 Neuroscience Forum : Abstracts. 2022;82.
https://hdl.handle.net/21.15107/rcub_vinar_12741 .

Bobić, Katarina, Todorović, Ana, Pejić, Snežana, Veljković, Filip, Pajović, Snežana, Nedeljković, Jovan, Drakulić, Dunja, "Surface modification of TiO2 nanoparticles moderates neurological impairments and upregulated oxidative stress indicator in eyes of adult Wistar rats" in Acta Neurobiologiae experimentalis : NEURONUS 2022 : 12th Neuronus 2022 Neuroscience Forum : Abstracts, 82 (2022),
https://hdl.handle.net/21.15107/rcub_vinar_12741 .

TY  - CONF
AU  - Valenta Šobot, Ana
AU  - Filipović Tričković, Jelena
AU  - Drakulić, Dunja
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Momić, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11582
AB  - Sweroside (Sw) is a secondary metabolite commonly found in plants belonging to the Gentianaceae family [1]. This iridoid compound is well-known for its anti-inflammatory [2], antidiabetic [3] and antitumor properties [4], which have been studied in pathological model systems. In transformed cell lines, Sw displays an antitumor effect by apoptosis activation [5]. Since healthy cells are also exposed to cancer therapy applied in vivo, our goal was to determine Sw’s cytotoxic concentration in primary human peripheral blood mononuclear cells (PBMCs) after 48 h of treatment with a range of concentration from 20 μM to 130 μM Sw in vitro, and to analyze whether the cytotoxic effect was due to activated apoptosis. According to the obtained results of the trypan blue dye exclusion test, 48 h of treatment with 50 μM Sw and higher concentrations led to a significant decrease in cell number. The DNA fragmentation assay indicated that following 50 μM Sw treatment, cells are dying in an apoptosis-like manner since the level of DNA fragments was 3.5 times higher than in the untreated control. The type of cell death was confirmed by immunoblot analysis of apoptosis- specific protein markers, which revealed the elevation of cleaved caspase-3 and PARP1 89 kDa fragments. Our findings showed that like in transformed cell lines, Sw in healthy cells can also activate apoptosis. A potential difference in sensitivity to Sw treatment between healthy and transformed cells could justify Sw treatment in anticancer therapy.
PB  - Belgrade : University of Belgrade, Faculty of Agriculture
C3  - 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
T1  - Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells
SP  - 47
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11582
ER  - 

@conference{
author = "Valenta Šobot, Ana and Filipović Tričković, Jelena and Drakulić, Dunja and Leskovac, Andreja and Petrović, Sandra and Momić, Tatjana",
year = "2022",
abstract = "Sweroside (Sw) is a secondary metabolite commonly found in plants belonging to the Gentianaceae family [1]. This iridoid compound is well-known for its anti-inflammatory [2], antidiabetic [3] and antitumor properties [4], which have been studied in pathological model systems. In transformed cell lines, Sw displays an antitumor effect by apoptosis activation [5]. Since healthy cells are also exposed to cancer therapy applied in vivo, our goal was to determine Sw’s cytotoxic concentration in primary human peripheral blood mononuclear cells (PBMCs) after 48 h of treatment with a range of concentration from 20 μM to 130 μM Sw in vitro, and to analyze whether the cytotoxic effect was due to activated apoptosis. According to the obtained results of the trypan blue dye exclusion test, 48 h of treatment with 50 μM Sw and higher concentrations led to a significant decrease in cell number. The DNA fragmentation assay indicated that following 50 μM Sw treatment, cells are dying in an apoptosis-like manner since the level of DNA fragments was 3.5 times higher than in the untreated control. The type of cell death was confirmed by immunoblot analysis of apoptosis- specific protein markers, which revealed the elevation of cleaved caspase-3 and PARP1 89 kDa fragments. Our findings showed that like in transformed cell lines, Sw in healthy cells can also activate apoptosis. A potential difference in sensitivity to Sw treatment between healthy and transformed cells could justify Sw treatment in anticancer therapy.",
publisher = "Belgrade : University of Belgrade, Faculty of Agriculture",
journal = "1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts",
title = "Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells",
pages = "47-47",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11582"
}

Valenta Šobot, A., Filipović Tričković, J., Drakulić, D., Leskovac, A., Petrović, S.,& Momić, T.. (2022). Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
Belgrade : University of Belgrade, Faculty of Agriculture., 47-47.
https://hdl.handle.net/21.15107/rcub_vinar_11582

Valenta Šobot A, Filipović Tričković J, Drakulić D, Leskovac A, Petrović S, Momić T. Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts. 2022;:47-47.
https://hdl.handle.net/21.15107/rcub_vinar_11582 .

Valenta Šobot, Ana, Filipović Tričković, Jelena, Drakulić, Dunja, Leskovac, Andreja, Petrović, Sandra, Momić, Tatjana, "Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells" in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts (2022):47-47,
https://hdl.handle.net/21.15107/rcub_vinar_11582 .

TY  - CONF
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Bobić, Katarina
AU  - Milovanović, Tijana
AU  - Stanković, Sanja
AU  - Drakulić, Dunja
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12742
AB  - Titanium dioxide nanoparticles (TiO2 NPs) are commonly used in various industrial and consumer products, including cosmetics, pharmaceuticals, food and beverages, etc. Since bare TiO2 NPs pose a risk to reproductive health, we compared their toxic effect to effect of TiO2 NPs surface-modified with salicylic acid (TiO2/SA NPs) or 5-amino salicylic acid (TiO2/5-ASA NPs) in female rats 14 days after acute oral treatment. In rat ovaries, oviducts, and uterus, bare TiO2 NPs and TiO2/SA NPs promoted acute toxicity along with the hormonal imbalance, irregular estrous cycle, swelling of the genital organs that increased organ-to-body mass ratio accompanied with prooxidative shift in redox status. The observed effects were less pronounced in TiO2/5-ASA NPs-treated rats. According to the presented results, surface-binding of 5-ASA, but not SA are able to suppress the toxic effects of bare TiO2 NPs in rat female reproductive system. Further studies are necessary to confirm these assumptions.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts
T1  - Acute oral intake of surface-modified TiO2 nanoparticles promotes biochemical and morphological changes in rat female reproductive system
SP  - 73
EP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12742
ER  - 

@conference{
author = "Todorović, Ana and Pejić, Snežana and Bobić, Katarina and Milovanović, Tijana and Stanković, Sanja and Drakulić, Dunja",
year = "2022",
abstract = "Titanium dioxide nanoparticles (TiO2 NPs) are commonly used in various industrial and consumer products, including cosmetics, pharmaceuticals, food and beverages, etc. Since bare TiO2 NPs pose a risk to reproductive health, we compared their toxic effect to effect of TiO2 NPs surface-modified with salicylic acid (TiO2/SA NPs) or 5-amino salicylic acid (TiO2/5-ASA NPs) in female rats 14 days after acute oral treatment. In rat ovaries, oviducts, and uterus, bare TiO2 NPs and TiO2/SA NPs promoted acute toxicity along with the hormonal imbalance, irregular estrous cycle, swelling of the genital organs that increased organ-to-body mass ratio accompanied with prooxidative shift in redox status. The observed effects were less pronounced in TiO2/5-ASA NPs-treated rats. According to the presented results, surface-binding of 5-ASA, but not SA are able to suppress the toxic effects of bare TiO2 NPs in rat female reproductive system. Further studies are necessary to confirm these assumptions.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts",
title = "Acute oral intake of surface-modified TiO2 nanoparticles promotes biochemical and morphological changes in rat female reproductive system",
pages = "73-73",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12742"
}

Todorović, A., Pejić, S., Bobić, K., Milovanović, T., Stanković, S.,& Drakulić, D.. (2022). Acute oral intake of surface-modified TiO2 nanoparticles promotes biochemical and morphological changes in rat female reproductive system. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts
Belgrade : Society of Physical Chemists of Serbia., 73-73.
https://hdl.handle.net/21.15107/rcub_vinar_12742

Todorović A, Pejić S, Bobić K, Milovanović T, Stanković S, Drakulić D. Acute oral intake of surface-modified TiO2 nanoparticles promotes biochemical and morphological changes in rat female reproductive system. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts. 2022;:73-73.
https://hdl.handle.net/21.15107/rcub_vinar_12742 .

Todorović, Ana, Pejić, Snežana, Bobić, Katarina, Milovanović, Tijana, Stanković, Sanja, Drakulić, Dunja, "Acute oral intake of surface-modified TiO2 nanoparticles promotes biochemical and morphological changes in rat female reproductive system" in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts (2022):73-73,
https://hdl.handle.net/21.15107/rcub_vinar_12742 .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Ružičić, Aleksandra
AU  - Lakić, Iva
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Glumac, Sofija
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Drakulić, Dunja R.
AU  - Stanković, Sanja
AU  - Jasnić, Nebojša
AU  - Đorđević, Jelena
AU  - Todorović, Zoran
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10173
AB  - A dysregulated and overwhelming response to an infection accompanied by the exaggerated pro-inflammatory state and metabolism disturbance leads to the fatal outcome in sepsis. Previously we showed that meldonium, an anti-ischemic drug clinically used to treat myocardial and cerebral ischemia, strongly increases mortality in faecal-induced peritonitis (FIP) in rats. We postulated that the same mechanism that is responsible for the otherwise strong anti-inflammatory effects of meldonium could be the culprit of the increased mortality. In the present study, we applied the LPS-induced model of sepsis to explore the presence of any differences from and/or similarities to the FIP model. When it comes to energy production, despite some shared similarities, it is evident that LPS and FIP models of sepsis differ greatly. A different profile of sympathoadrenal activation may account for this observation, as it was lacking in the FIP model, whereas in the LPS model it was strong enough to overcome the effects of meldonium. Therefore, choosing the appropriate model of sepsis induction is of great importance, especially if energy homeostasis is the main focus of the study. Even when differences in the experimental design of the two models are acknowledged, the role of different patterns of energy production cannot be excluded. On that account, our results draw attention to the importance of uninterrupted energy production in sepsis but also call for much-needed revisions of the current recommendations for its treatment.
T2  - International Journal of Molecular Sciences
T1  - The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats
VL  - 23
IS  - 4
SP  - 2395
DO  - 10.3390/ijms23042395
ER  - 

@article{
author = "Đurašević, Siniša and Ružičić, Aleksandra and Lakić, Iva and Tosti, Tomislav and Đurović, Saša and Glumac, Sofija and Pejić, Snežana and Todorović, Ana and Drakulić, Dunja R. and Stanković, Sanja and Jasnić, Nebojša and Đorđević, Jelena and Todorović, Zoran",
year = "2022",
abstract = "A dysregulated and overwhelming response to an infection accompanied by the exaggerated pro-inflammatory state and metabolism disturbance leads to the fatal outcome in sepsis. Previously we showed that meldonium, an anti-ischemic drug clinically used to treat myocardial and cerebral ischemia, strongly increases mortality in faecal-induced peritonitis (FIP) in rats. We postulated that the same mechanism that is responsible for the otherwise strong anti-inflammatory effects of meldonium could be the culprit of the increased mortality. In the present study, we applied the LPS-induced model of sepsis to explore the presence of any differences from and/or similarities to the FIP model. When it comes to energy production, despite some shared similarities, it is evident that LPS and FIP models of sepsis differ greatly. A different profile of sympathoadrenal activation may account for this observation, as it was lacking in the FIP model, whereas in the LPS model it was strong enough to overcome the effects of meldonium. Therefore, choosing the appropriate model of sepsis induction is of great importance, especially if energy homeostasis is the main focus of the study. Even when differences in the experimental design of the two models are acknowledged, the role of different patterns of energy production cannot be excluded. On that account, our results draw attention to the importance of uninterrupted energy production in sepsis but also call for much-needed revisions of the current recommendations for its treatment.",
journal = "International Journal of Molecular Sciences",
title = "The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats",
volume = "23",
number = "4",
pages = "2395",
doi = "10.3390/ijms23042395"
}

Đurašević, S., Ružičić, A., Lakić, I., Tosti, T., Đurović, S., Glumac, S., Pejić, S., Todorović, A., Drakulić, D. R., Stanković, S., Jasnić, N., Đorđević, J.,& Todorović, Z.. (2022). The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats. in International Journal of Molecular Sciences, 23(4), 2395.
https://doi.org/10.3390/ijms23042395

Đurašević S, Ružičić A, Lakić I, Tosti T, Đurović S, Glumac S, Pejić S, Todorović A, Drakulić DR, Stanković S, Jasnić N, Đorđević J, Todorović Z. The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats. in International Journal of Molecular Sciences. 2022;23(4):2395.
doi:10.3390/ijms23042395 .

Đurašević, Siniša, Ružičić, Aleksandra, Lakić, Iva, Tosti, Tomislav, Đurović, Saša, Glumac, Sofija, Pejić, Snežana, Todorović, Ana, Drakulić, Dunja R., Stanković, Sanja, Jasnić, Nebojša, Đorđević, Jelena, Todorović, Zoran, "The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats" in International Journal of Molecular Sciences, 23, no. 4 (2022):2395,
https://doi.org/10.3390/ijms23042395 . .

TY  - JOUR
AU  - Pantić, Igor
AU  - Paunović, Jovana
AU  - Pejić, Snežana
AU  - Drakulić, Dunja R.
AU  - Todorović, Ana
AU  - Stanković, Sanja
AU  - Vučević, Danijela
AU  - Cumic, Jelena
AU  - Radosavljević, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10212
AB  - Artificial intelligence (AI) and machine learning models are today frequently used for classification and prediction of various biochemical processes and phenomena. In recent years, numerous research efforts have been focused on developing such models for assessment, categorization, and prediction of oxidative stress. Supervised machine learning can successfully automate the process of evaluation and quantification of oxidative damage in biological samples, as well as extract useful data from the abundance of experimental results. In this concise review, we cover the possible applications of neural networks, decision trees and regression analysis as three common strategies in machine learning. We also review recent works on the various weaknesses and limitations of artificial intelligence in biochemistry and related scientific areas. Finally, we discuss future innovative approaches on the ways how AI can contribute to the automation of oxidative stress measurement and diagnosis of diseases associated with oxidative damage.
T2  - Chemico-Biological Interactions
T2  - Chemico-Biological Interactions
T1  - Artificial intelligence approaches to the biochemistry of oxidative stress: Current state of the art
VL  - 358
SP  - 109888
DO  - 10.1016/j.cbi.2022.109888
ER  - 

@article{
author = "Pantić, Igor and Paunović, Jovana and Pejić, Snežana and Drakulić, Dunja R. and Todorović, Ana and Stanković, Sanja and Vučević, Danijela and Cumic, Jelena and Radosavljević, Tatjana",
year = "2022",
abstract = "Artificial intelligence (AI) and machine learning models are today frequently used for classification and prediction of various biochemical processes and phenomena. In recent years, numerous research efforts have been focused on developing such models for assessment, categorization, and prediction of oxidative stress. Supervised machine learning can successfully automate the process of evaluation and quantification of oxidative damage in biological samples, as well as extract useful data from the abundance of experimental results. In this concise review, we cover the possible applications of neural networks, decision trees and regression analysis as three common strategies in machine learning. We also review recent works on the various weaknesses and limitations of artificial intelligence in biochemistry and related scientific areas. Finally, we discuss future innovative approaches on the ways how AI can contribute to the automation of oxidative stress measurement and diagnosis of diseases associated with oxidative damage.",
journal = "Chemico-Biological Interactions, Chemico-Biological Interactions",
title = "Artificial intelligence approaches to the biochemistry of oxidative stress: Current state of the art",
volume = "358",
pages = "109888",
doi = "10.1016/j.cbi.2022.109888"
}

Pantić, I., Paunović, J., Pejić, S., Drakulić, D. R., Todorović, A., Stanković, S., Vučević, D., Cumic, J.,& Radosavljević, T.. (2022). Artificial intelligence approaches to the biochemistry of oxidative stress: Current state of the art. in Chemico-Biological Interactions, 358, 109888.
https://doi.org/10.1016/j.cbi.2022.109888

Pantić I, Paunović J, Pejić S, Drakulić DR, Todorović A, Stanković S, Vučević D, Cumic J, Radosavljević T. Artificial intelligence approaches to the biochemistry of oxidative stress: Current state of the art. in Chemico-Biological Interactions. 2022;358:109888.
doi:10.1016/j.cbi.2022.109888 .

Pantić, Igor, Paunović, Jovana, Pejić, Snežana, Drakulić, Dunja R., Todorović, Ana, Stanković, Sanja, Vučević, Danijela, Cumic, Jelena, Radosavljević, Tatjana, "Artificial intelligence approaches to the biochemistry of oxidative stress: Current state of the art" in Chemico-Biological Interactions, 358 (2022):109888,
https://doi.org/10.1016/j.cbi.2022.109888 . .

TY  - CONF
AU  - Bobić, Katarina
AU  - Guševac Stojanović, Ivana
AU  - Todorović, Ana
AU  - Veljković, Filip
AU  - Pejić, Snežana
AU  - Martinović, Jelena
AU  - Drakulić, Dunja
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11007
AB  - Progesterone (P4), a naturally occurring gonadal hormone in the brain, and its metabolites, are proposed as potential therapeutic agents in various neurodegenerative animal models given that their neuroprotective properties might be associated with amelioration of oxidative stress. Since P4 actions in rat striatum upon permanent ligation of both common carotid arteries are still ambiguous, the present study aimed to evaluate whether 7 days lasting P4 treatment could modulate the levels of several striatal oxidative stress indicators, including prooxidant/antioxidant balance (PAB), advanced oxidation protein products (AOPP) and products of lipid peroxidation (LPO). For the purpose of the experiment, adult male Wistar rats (n = 12) were divided into 3 groups: sham-operated animals subjected to vehicle (commercial flax oil, 1 mg/kg, s.c., Sham + V), occluded animals treated either with vehicle (2VO + V) or P4 (dissolved in commercial flax oil, 1.7 mg/kg, s.c., 2VO + P4). Rats were sacrificed 4 h following the last treatment1 and striatal synaptosomal fraction was used for further biochemical analyses 2. Our results demonstrate that investigated oxidative stress indicators are affected to the different extents by P4 treatment. Namely, in comparison to the Sham + V group, PAB level was elevated in 2VO + V rats, while in 2VO + P4 animals it was downregulated to the levels observed in the Sham + V group. In parallel, 2VO-induced alteration of AOPP was decreased following P4 treatment whereas LPO level was still slightly elevated. Overall, our findings suggest that P4 might manifest antioxidative features in the striatum of hypoperfused rats.
PB  - Belgrade : Faculty of Chemistry : Serbian Biochemical Society
C3  - Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia
T1  - Antioxidative properties of progesterone in striatum of permanently occluded adult male Wistar rats
SP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11007
ER  - 

@conference{
author = "Bobić, Katarina and Guševac Stojanović, Ivana and Todorović, Ana and Veljković, Filip and Pejić, Snežana and Martinović, Jelena and Drakulić, Dunja",
year = "2022",
abstract = "Progesterone (P4), a naturally occurring gonadal hormone in the brain, and its metabolites, are proposed as potential therapeutic agents in various neurodegenerative animal models given that their neuroprotective properties might be associated with amelioration of oxidative stress. Since P4 actions in rat striatum upon permanent ligation of both common carotid arteries are still ambiguous, the present study aimed to evaluate whether 7 days lasting P4 treatment could modulate the levels of several striatal oxidative stress indicators, including prooxidant/antioxidant balance (PAB), advanced oxidation protein products (AOPP) and products of lipid peroxidation (LPO). For the purpose of the experiment, adult male Wistar rats (n = 12) were divided into 3 groups: sham-operated animals subjected to vehicle (commercial flax oil, 1 mg/kg, s.c., Sham + V), occluded animals treated either with vehicle (2VO + V) or P4 (dissolved in commercial flax oil, 1.7 mg/kg, s.c., 2VO + P4). Rats were sacrificed 4 h following the last treatment1 and striatal synaptosomal fraction was used for further biochemical analyses 2. Our results demonstrate that investigated oxidative stress indicators are affected to the different extents by P4 treatment. Namely, in comparison to the Sham + V group, PAB level was elevated in 2VO + V rats, while in 2VO + P4 animals it was downregulated to the levels observed in the Sham + V group. In parallel, 2VO-induced alteration of AOPP was decreased following P4 treatment whereas LPO level was still slightly elevated. Overall, our findings suggest that P4 might manifest antioxidative features in the striatum of hypoperfused rats.",
publisher = "Belgrade : Faculty of Chemistry : Serbian Biochemical Society",
journal = "Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia",
title = "Antioxidative properties of progesterone in striatum of permanently occluded adult male Wistar rats",
pages = "53",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11007"
}

Bobić, K., Guševac Stojanović, I., Todorović, A., Veljković, F., Pejić, S., Martinović, J.,& Drakulić, D.. (2022). Antioxidative properties of progesterone in striatum of permanently occluded adult male Wistar rats. in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia
Belgrade : Faculty of Chemistry : Serbian Biochemical Society., 53.
https://hdl.handle.net/21.15107/rcub_vinar_11007

Bobić K, Guševac Stojanović I, Todorović A, Veljković F, Pejić S, Martinović J, Drakulić D. Antioxidative properties of progesterone in striatum of permanently occluded adult male Wistar rats. in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia. 2022;:53.
https://hdl.handle.net/21.15107/rcub_vinar_11007 .

Bobić, Katarina, Guševac Stojanović, Ivana, Todorović, Ana, Veljković, Filip, Pejić, Snežana, Martinović, Jelena, Drakulić, Dunja, "Antioxidative properties of progesterone in striatum of permanently occluded adult male Wistar rats" in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia (2022):53,
https://hdl.handle.net/21.15107/rcub_vinar_11007 .

TY  - JOUR
AU  - Glumac, Sofija
AU  - Davidović, Radoslav S.
AU  - Dožić, Branko
AU  - Hinić, Sasa
AU  - Pavlović, Ivan
AU  - Drakulić, Dunja R.
AU  - Todorović, Ana
AU  - Medojević Pavlović, Maja
AU  - Radojević-Škodrić, Sanja
AU  - Baralić, Ivana
AU  - Sopta, Jelena
AU  - Pejić, Snežana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9887
AB  - Rhabdomyosarcoma (RMS) is a highly malignant cancer and is the most common soft tissue sarcoma in children and adolescents, but it is rare in adults (<1% of all adult malignancies). Altered expression and molecular abnormalities of cell-cycle-regulatory proteins are one of the most prominent features in RMS. Therefore, we evaluated the expression of cyclin-dependent kinase inhibitors p57 and p16, as well as p16 methylation status, along with clinicopathological characteristics and overall survival (OS) in RMS patients. This analysis was conducted on 23 pediatric and 44 adult patients. There was a male predominance in both groups and extremities were the most frequent tumor site. In adults, alveolar and pleomorphic types were almost equally represented. The majority of pediatric tumors were low grade, whereas, in adults, only one patient had a low-grade tumor. Seven pediatric (30.43%) and eight adult (18.18%) patients had a low p16 expression. The analysis of methylation status of the p16 promoter showed the presence of methylated allele only in one sample with pleomorphic histology. Six (26.1%) pediatric and 15 (34.1%) adult patients had low p57 expression, while in 17 (73.9%) pediatric and 29 (65.9%) adult patients it was assessed as high. Ninetyone percent of the pediatric patients and 32.6% of adults were alive at the end of the observational period. In adults, significant associations were found between OS and age (P = 0.020), gender (P = 0.027), tumor size (P < 0.001), lymph node status (P < 0.001), presence of metastases (P = 0.015), and p57 expression (P = 0.039). Stratification by histological type showed the correlation of low p57 expression (P = 0.030) and worse OS of patients with alveolar RMS. Univariate analysis identified age > 50 yrs. (HR 2.447), tumors > 5 cm (HR 21.31), involvement of regional lymph nodes (HR 3.96), the presence of metastases (HR 2.53), and low p57 expression (HR 2.11) as predictors of lower OS. Tumor size, regional lymph nodes involvement, and metastases were the independent predictors after multivariate analysis, while p57 did not predict OS in an independent way. In summary, although p57 was not confirmed to be an independent predictor of OS, our results indicate that its low expression may be the marker of aggressive phenotype and poor prognosis in adult RMS patients. Also, our findings suggest that epigenetic inactivation of p16 is not important in the pathogenesis of rhabdomyosarcoma.
T2  - Pathology - Research and Practice
T1  - Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma
VL  - 225
SP  - 153558
DO  - 10.1016/j.prp.2021.153558
ER  - 

@article{
author = "Glumac, Sofija and Davidović, Radoslav S. and Dožić, Branko and Hinić, Sasa and Pavlović, Ivan and Drakulić, Dunja R. and Todorović, Ana and Medojević Pavlović, Maja and Radojević-Škodrić, Sanja and Baralić, Ivana and Sopta, Jelena and Pejić, Snežana",
year = "2021",
abstract = "Rhabdomyosarcoma (RMS) is a highly malignant cancer and is the most common soft tissue sarcoma in children and adolescents, but it is rare in adults (<1% of all adult malignancies). Altered expression and molecular abnormalities of cell-cycle-regulatory proteins are one of the most prominent features in RMS. Therefore, we evaluated the expression of cyclin-dependent kinase inhibitors p57 and p16, as well as p16 methylation status, along with clinicopathological characteristics and overall survival (OS) in RMS patients. This analysis was conducted on 23 pediatric and 44 adult patients. There was a male predominance in both groups and extremities were the most frequent tumor site. In adults, alveolar and pleomorphic types were almost equally represented. The majority of pediatric tumors were low grade, whereas, in adults, only one patient had a low-grade tumor. Seven pediatric (30.43%) and eight adult (18.18%) patients had a low p16 expression. The analysis of methylation status of the p16 promoter showed the presence of methylated allele only in one sample with pleomorphic histology. Six (26.1%) pediatric and 15 (34.1%) adult patients had low p57 expression, while in 17 (73.9%) pediatric and 29 (65.9%) adult patients it was assessed as high. Ninetyone percent of the pediatric patients and 32.6% of adults were alive at the end of the observational period. In adults, significant associations were found between OS and age (P = 0.020), gender (P = 0.027), tumor size (P < 0.001), lymph node status (P < 0.001), presence of metastases (P = 0.015), and p57 expression (P = 0.039). Stratification by histological type showed the correlation of low p57 expression (P = 0.030) and worse OS of patients with alveolar RMS. Univariate analysis identified age > 50 yrs. (HR 2.447), tumors > 5 cm (HR 21.31), involvement of regional lymph nodes (HR 3.96), the presence of metastases (HR 2.53), and low p57 expression (HR 2.11) as predictors of lower OS. Tumor size, regional lymph nodes involvement, and metastases were the independent predictors after multivariate analysis, while p57 did not predict OS in an independent way. In summary, although p57 was not confirmed to be an independent predictor of OS, our results indicate that its low expression may be the marker of aggressive phenotype and poor prognosis in adult RMS patients. Also, our findings suggest that epigenetic inactivation of p16 is not important in the pathogenesis of rhabdomyosarcoma.",
journal = "Pathology - Research and Practice",
title = "Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma",
volume = "225",
pages = "153558",
doi = "10.1016/j.prp.2021.153558"
}

Glumac, S., Davidović, R. S., Dožić, B., Hinić, S., Pavlović, I., Drakulić, D. R., Todorović, A., Medojević Pavlović, M., Radojević-Škodrić, S., Baralić, I., Sopta, J.,& Pejić, S.. (2021). Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma. in Pathology - Research and Practice, 225, 153558.
https://doi.org/10.1016/j.prp.2021.153558

Glumac S, Davidović RS, Dožić B, Hinić S, Pavlović I, Drakulić DR, Todorović A, Medojević Pavlović M, Radojević-Škodrić S, Baralić I, Sopta J, Pejić S. Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma. in Pathology - Research and Practice. 2021;225:153558.
doi:10.1016/j.prp.2021.153558 .

Glumac, Sofija, Davidović, Radoslav S., Dožić, Branko, Hinić, Sasa, Pavlović, Ivan, Drakulić, Dunja R., Todorović, Ana, Medojević Pavlović, Maja, Radojević-Škodrić, Sanja, Baralić, Ivana, Sopta, Jelena, Pejić, Snežana, "Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma" in Pathology - Research and Practice, 225 (2021):153558,
https://doi.org/10.1016/j.prp.2021.153558 . .

TY  - CONF
AU  - Veljković, Filip M.
AU  - Radović, Ivana M.
AU  - Drakulić, Dunja R.
AU  - Dimitrijević, Stevan
AU  - Dimitrijević, Silvana
AU  - Stoiljković, Milovan
AU  - Veličković, Suzana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10789
AB  - Silver alloys are generally used in the different fields of industry, including chemical processing, construction, heat exchangers, etc. Although Ag-Cu-Zn-Cd type of alloys, were widely exploited in the second half of the 20th century for their excellent properties, its use is forbidden in the EU due to the high toxicity of cadmium vapors. Ag-Cu-In type of alloy is a good alternative to Ag-Cu-Zn-Cd alloys, with great properties and can be used in various fields of industry. Most common methods for characterization of surface corrosion films of Ag-Cu alloys are: X-ray diffraction (XRD), scanning electron microscopy with energy dispersion spectroscopy (SEM-EDS), Raman spectroscopy and atomic force microscopy (AFM). Our current study focuses on the application of laser desorption ionization mass spectrometry (LDI MS) for determination of corrosion products of Ag-Cu alloy. The aim of this study was to confirm LDI MS as a fast, accurate and reliable method for determination of corrosion products on the surface of Ag-Cu-In alloy.
PB  - Belgrade : Serbian Ceramic Society
C3  - Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade
T1  - Determination of corrosion products of Ag-Cu alloy by laser desorption ionization mass spectrometry
SP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10789
ER  - 

@conference{
author = "Veljković, Filip M. and Radović, Ivana M. and Drakulić, Dunja R. and Dimitrijević, Stevan and Dimitrijević, Silvana and Stoiljković, Milovan and Veličković, Suzana",
year = "2021",
abstract = "Silver alloys are generally used in the different fields of industry, including chemical processing, construction, heat exchangers, etc. Although Ag-Cu-Zn-Cd type of alloys, were widely exploited in the second half of the 20th century for their excellent properties, its use is forbidden in the EU due to the high toxicity of cadmium vapors. Ag-Cu-In type of alloy is a good alternative to Ag-Cu-Zn-Cd alloys, with great properties and can be used in various fields of industry. Most common methods for characterization of surface corrosion films of Ag-Cu alloys are: X-ray diffraction (XRD), scanning electron microscopy with energy dispersion spectroscopy (SEM-EDS), Raman spectroscopy and atomic force microscopy (AFM). Our current study focuses on the application of laser desorption ionization mass spectrometry (LDI MS) for determination of corrosion products of Ag-Cu alloy. The aim of this study was to confirm LDI MS as a fast, accurate and reliable method for determination of corrosion products on the surface of Ag-Cu-In alloy.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade",
title = "Determination of corrosion products of Ag-Cu alloy by laser desorption ionization mass spectrometry",
pages = "50",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10789"
}

Veljković, F. M., Radović, I. M., Drakulić, D. R., Dimitrijević, S., Dimitrijević, S., Stoiljković, M.,& Veličković, S.. (2021). Determination of corrosion products of Ag-Cu alloy by laser desorption ionization mass spectrometry. in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade
Belgrade : Serbian Ceramic Society., 50.
https://hdl.handle.net/21.15107/rcub_vinar_10789

Veljković FM, Radović IM, Drakulić DR, Dimitrijević S, Dimitrijević S, Stoiljković M, Veličković S. Determination of corrosion products of Ag-Cu alloy by laser desorption ionization mass spectrometry. in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade. 2021;:50.
https://hdl.handle.net/21.15107/rcub_vinar_10789 .

Veljković, Filip M., Radović, Ivana M., Drakulić, Dunja R., Dimitrijević, Stevan, Dimitrijević, Silvana, Stoiljković, Milovan, Veličković, Suzana, "Determination of corrosion products of Ag-Cu alloy by laser desorption ionization mass spectrometry" in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade (2021):50,
https://hdl.handle.net/21.15107/rcub_vinar_10789 .

TY  - CONF
AU  - Todorović, Ana
AU  - Drakulić, Dunja
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Popović, Nataša M.
AU  - Đorđević, Neda
AU  - Todorović Vukotić, Nevena
AU  - Miletić, J.
AU  - Pejić, Snežana
AU  - Pajović, Snežana B.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12580
AB  - The literature emphasizes the involvement of oxidative stress in the etiopathogenesis of many uterine diseases. Antioxidant system (AOS) represents the protective mechanism used by cells to neutralize overproduced reactive oxygen species (ROS) and prevent oxidative stress. We have previously shown that in gynecological patients with various diagnoses, the reproductive and other factors may be associated with antioxidant capacity and the ability to defend against oxidative damage. In this study, we examined the changes in expression of antioxidant enzymes (AOE): superoxide dismutases (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the blood of women with endometrial hyperplasia. Our results indicate that hyperplasia induces perturbance in oxidative balance, particularly in glutathione redox cycle enzymes.
PB  - The Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24
T1  - Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia
SP  - 308
EP  - 311
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12580
ER  - 

@conference{
author = "Todorović, Ana and Drakulić, Dunja and Gavrilović, Ljubica and Stojiljković, Vesna and Popović, Nataša M. and Đorđević, Neda and Todorović Vukotić, Nevena and Miletić, J. and Pejić, Snežana and Pajović, Snežana B.",
year = "2021",
abstract = "The literature emphasizes the involvement of oxidative stress in the etiopathogenesis of many uterine diseases. Antioxidant system (AOS) represents the protective mechanism used by cells to neutralize overproduced reactive oxygen species (ROS) and prevent oxidative stress. We have previously shown that in gynecological patients with various diagnoses, the reproductive and other factors may be associated with antioxidant capacity and the ability to defend against oxidative damage. In this study, we examined the changes in expression of antioxidant enzymes (AOE): superoxide dismutases (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the blood of women with endometrial hyperplasia. Our results indicate that hyperplasia induces perturbance in oxidative balance, particularly in glutathione redox cycle enzymes.",
publisher = "The Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24",
title = "Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia",
pages = "308-311",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12580"
}

Todorović, A., Drakulić, D., Gavrilović, L., Stojiljković, V., Popović, N. M., Đorđević, N., Todorović Vukotić, N., Miletić, J., Pejić, S.,& Pajović, S. B.. (2021). Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24
The Society of Physical Chemists of Serbia., 308-311.
https://hdl.handle.net/21.15107/rcub_vinar_12580

Todorović A, Drakulić D, Gavrilović L, Stojiljković V, Popović NM, Đorđević N, Todorović Vukotić N, Miletić J, Pejić S, Pajović SB. Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24. 2021;:308-311.
https://hdl.handle.net/21.15107/rcub_vinar_12580 .

Todorović, Ana, Drakulić, Dunja, Gavrilović, Ljubica, Stojiljković, Vesna, Popović, Nataša M., Đorđević, Neda, Todorović Vukotić, Nevena, Miletić, J., Pejić, Snežana, Pajović, Snežana B., "Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24 (2021):308-311,
https://hdl.handle.net/21.15107/rcub_vinar_12580 .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Pejić, Snežana
AU  - Grigorov, Ilijana
AU  - Nikolić, Gorana
AU  - Mitić-Ćulafić, Dragana
AU  - Dragićević, Milan
AU  - Đorđević, Jelena
AU  - Todorović Vukotić, Nevena
AU  - Đorđević, Neda O.
AU  - Todorović, Ana
AU  - Drakulić, Dunja R.
AU  - Veljković, Filip M.
AU  - Pajović, Snežana B.
AU  - Todorović, Zoran
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9831
AB  - Thioacetamide (TAA) is widely used to study liver toxicity accompanied by oxidative stress, inflammation, cell necrosis, fibrosis, cholestasis, and hepatocellular carcinoma. As an efficient free radical’s scavenger, C60 fullerene is considered a potential liver-protective agent in chemically-induced liver injury. In the present work, we examined the hepatoprotective effects of two C60 doses dissolved in virgin olive oil against TAA-induced hepatotoxicity in rats. We showed that TAA-induced increase in liver oxidative stress, judged by the changes in the activities of SOD, CAT, GPx, GR, GST, the content of GSH and 4-HNE, and expression of HO-1, MnSOD, and CuZnSOD, was more effectively ameliorated with a lower C60 dose. Improvement in liver antioxidative status caused by C60 was accompanied by a decrease in liver HMGB1 expression and an increase in nuclear Nrf2/NF-κB p65 ratio, suggesting a reduction in inflammation, necrosis and fibrosis. These results were in accordance with liver histology analysis, liver comet assay, and changes in serum levels of ALT, AST, and AP. The changes observed in gut microbiome support detrimental effects of TAA and hepatoprotective effects of low C60 dose. Less protective effects of a higher C60 dose could be a consequence of its enhanced aggregation and related pro-oxidant role.
T2  - Antioxidants
T1  - Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes
VL  - 10
IS  - 6
SP  - 911
DO  - 10.3390/antiox10060911
ER  - 

@article{
author = "Đurašević, Siniša and Pejić, Snežana and Grigorov, Ilijana and Nikolić, Gorana and Mitić-Ćulafić, Dragana and Dragićević, Milan and Đorđević, Jelena and Todorović Vukotić, Nevena and Đorđević, Neda O. and Todorović, Ana and Drakulić, Dunja R. and Veljković, Filip M. and Pajović, Snežana B. and Todorović, Zoran",
year = "2021",
abstract = "Thioacetamide (TAA) is widely used to study liver toxicity accompanied by oxidative stress, inflammation, cell necrosis, fibrosis, cholestasis, and hepatocellular carcinoma. As an efficient free radical’s scavenger, C60 fullerene is considered a potential liver-protective agent in chemically-induced liver injury. In the present work, we examined the hepatoprotective effects of two C60 doses dissolved in virgin olive oil against TAA-induced hepatotoxicity in rats. We showed that TAA-induced increase in liver oxidative stress, judged by the changes in the activities of SOD, CAT, GPx, GR, GST, the content of GSH and 4-HNE, and expression of HO-1, MnSOD, and CuZnSOD, was more effectively ameliorated with a lower C60 dose. Improvement in liver antioxidative status caused by C60 was accompanied by a decrease in liver HMGB1 expression and an increase in nuclear Nrf2/NF-κB p65 ratio, suggesting a reduction in inflammation, necrosis and fibrosis. These results were in accordance with liver histology analysis, liver comet assay, and changes in serum levels of ALT, AST, and AP. The changes observed in gut microbiome support detrimental effects of TAA and hepatoprotective effects of low C60 dose. Less protective effects of a higher C60 dose could be a consequence of its enhanced aggregation and related pro-oxidant role.",
journal = "Antioxidants",
title = "Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes",
volume = "10",
number = "6",
pages = "911",
doi = "10.3390/antiox10060911"
}

Đurašević, S., Pejić, S., Grigorov, I., Nikolić, G., Mitić-Ćulafić, D., Dragićević, M., Đorđević, J., Todorović Vukotić, N., Đorđević, N. O., Todorović, A., Drakulić, D. R., Veljković, F. M., Pajović, S. B.,& Todorović, Z.. (2021). Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes. in Antioxidants, 10(6), 911.
https://doi.org/10.3390/antiox10060911

Đurašević S, Pejić S, Grigorov I, Nikolić G, Mitić-Ćulafić D, Dragićević M, Đorđević J, Todorović Vukotić N, Đorđević NO, Todorović A, Drakulić DR, Veljković FM, Pajović SB, Todorović Z. Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes. in Antioxidants. 2021;10(6):911.
doi:10.3390/antiox10060911 .

Đurašević, Siniša, Pejić, Snežana, Grigorov, Ilijana, Nikolić, Gorana, Mitić-Ćulafić, Dragana, Dragićević, Milan, Đorđević, Jelena, Todorović Vukotić, Nevena, Đorđević, Neda O., Todorović, Ana, Drakulić, Dunja R., Veljković, Filip M., Pajović, Snežana B., Todorović, Zoran, "Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes" in Antioxidants, 10, no. 6 (2021):911,
https://doi.org/10.3390/antiox10060911 . .

TY  - CONF
AU  - Martinović, Jelena
AU  - Guševac Stojanović, Ivana
AU  - Zarić, M.
AU  - Todorović, Ana
AU  - Veljković, Filip
AU  - Pejić, Snežana
AU  - Stojanović, Zoran
AU  - Mitrović, N.
AU  - Grković, Ivana
AU  - Drakulić, Dunja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11399
AB  - D-galactose (d-gal) is an important physiological nutrient. According to thewidely accepted aging metabolic theory d-gal at high levels can be convertedinto aldose and hydroperoxide, resulting in the overproduction of reactiveoxygen species (ROS). Increased ROS levels may subsequently cause oxidative stress, inflammation, mitochondrial dysfunction, and apoptosis which are hallmarks of natural senescence as well as various pathological conditions. We investigated the effects of chronic oral d-gal intake (200 mg/kg and 500 mg/kg for 6 weeks) on physiological, neurological and toxicity parameters in 3 months old male Wistar rats. The obtained results indicate that body weight, food intake, serum glucose, neurological and toxicity status remained unaffected while urine proteins were significantly increased in d-gal treated rats. Although there was no effect on the general health status of the animals, our findings suggest that chronic oral d-gal administration may lead to renal dysfunction.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry
T1  - Effects of chronic oral D-galactose treatment on general health status in male Wistar rats
SP  - 115
EP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11399
ER  - 

@conference{
author = "Martinović, Jelena and Guševac Stojanović, Ivana and Zarić, M. and Todorović, Ana and Veljković, Filip and Pejić, Snežana and Stojanović, Zoran and Mitrović, N. and Grković, Ivana and Drakulić, Dunja",
year = "2021",
abstract = "D-galactose (d-gal) is an important physiological nutrient. According to thewidely accepted aging metabolic theory d-gal at high levels can be convertedinto aldose and hydroperoxide, resulting in the overproduction of reactiveoxygen species (ROS). Increased ROS levels may subsequently cause oxidative stress, inflammation, mitochondrial dysfunction, and apoptosis which are hallmarks of natural senescence as well as various pathological conditions. We investigated the effects of chronic oral d-gal intake (200 mg/kg and 500 mg/kg for 6 weeks) on physiological, neurological and toxicity parameters in 3 months old male Wistar rats. The obtained results indicate that body weight, food intake, serum glucose, neurological and toxicity status remained unaffected while urine proteins were significantly increased in d-gal treated rats. Although there was no effect on the general health status of the animals, our findings suggest that chronic oral d-gal administration may lead to renal dysfunction.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry",
title = "Effects of chronic oral D-galactose treatment on general health status in male Wistar rats",
pages = "115-118",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11399"
}

Martinović, J., Guševac Stojanović, I., Zarić, M., Todorović, A., Veljković, F., Pejić, S., Stojanović, Z., Mitrović, N., Grković, I.,& Drakulić, D.. (2021). Effects of chronic oral D-galactose treatment on general health status in male Wistar rats. in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry
Belgrade : Vinča Institute of Nuclear Sciences., 115-118.
https://hdl.handle.net/21.15107/rcub_vinar_11399

Martinović J, Guševac Stojanović I, Zarić M, Todorović A, Veljković F, Pejić S, Stojanović Z, Mitrović N, Grković I, Drakulić D. Effects of chronic oral D-galactose treatment on general health status in male Wistar rats. in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry. 2021;:115-118.
https://hdl.handle.net/21.15107/rcub_vinar_11399 .

Martinović, Jelena, Guševac Stojanović, Ivana, Zarić, M., Todorović, Ana, Veljković, Filip, Pejić, Snežana, Stojanović, Zoran, Mitrović, N., Grković, Ivana, Drakulić, Dunja, "Effects of chronic oral D-galactose treatment on general health status in male Wistar rats" in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry (2021):115-118,
https://hdl.handle.net/21.15107/rcub_vinar_11399 .