TY  - JOUR
AU  - Marković, Zoran M.
AU  - Ristić, Biljana Z.
AU  - Arsikin, Katarina M.
AU  - Klisic, Djordje G.
AU  - Harhaji-Trajković, Ljubica M.
AU  - Todorović-Marković, Biljana
AU  - Kepić, Dejan P.
AU  - Kravić-Stevović, Tamara K.
AU  - Jovanović, Svetlana P.
AU  - Milenković, Marina
AU  - Milivojević, Dušan
AU  - Bumbaširević, Vladimir Ž.
AU  - Dramićanin, Miroslav
AU  - Trajković, Vladimir S.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5025
AB  - The excellent photoluminescent properties of graphene quantum dots (GQD) makes them suitable candidates for biomedical applications, but their cytotoxicity has not been extensively studied. Here we show that electrochemically produced GQD irradiated with blue light (470 nm, 1 W) generate reactive oxygen species, including singlet oxygen, and kill U251 human glioma cells by causing oxidative stress. The cell death induced by photoexcited GQD displayed morphological and/or biochemical characteristics of both apoptosis (phosphatidylserine externalization, caspase activation. DNA fragmentation) and autophagy (formation of autophagic vesicles, LC3-I/LC3-II conversion, degradation of autophagic target p62). Moreover, a genetic inactivation of autophagy-essential LOB protein partly abrogated the photodynamic cytotoxicity of GQD. These data indicate potential usefulness of GQD in photodynamic therapy, but also raise concerns about their possible toxicity. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Biomaterials
T1  - Graphene quantum dots as autophagy-inducing photodynamic agents
VL  - 33
IS  - 29
SP  - 7084
EP  - 7092
DO  - 10.1016/j.biomaterials.2012.06.060
ER  - 

@article{
author = "Marković, Zoran M. and Ristić, Biljana Z. and Arsikin, Katarina M. and Klisic, Djordje G. and Harhaji-Trajković, Ljubica M. and Todorović-Marković, Biljana and Kepić, Dejan P. and Kravić-Stevović, Tamara K. and Jovanović, Svetlana P. and Milenković, Marina and Milivojević, Dušan and Bumbaširević, Vladimir Ž. and Dramićanin, Miroslav and Trajković, Vladimir S.",
year = "2012",
abstract = "The excellent photoluminescent properties of graphene quantum dots (GQD) makes them suitable candidates for biomedical applications, but their cytotoxicity has not been extensively studied. Here we show that electrochemically produced GQD irradiated with blue light (470 nm, 1 W) generate reactive oxygen species, including singlet oxygen, and kill U251 human glioma cells by causing oxidative stress. The cell death induced by photoexcited GQD displayed morphological and/or biochemical characteristics of both apoptosis (phosphatidylserine externalization, caspase activation. DNA fragmentation) and autophagy (formation of autophagic vesicles, LC3-I/LC3-II conversion, degradation of autophagic target p62). Moreover, a genetic inactivation of autophagy-essential LOB protein partly abrogated the photodynamic cytotoxicity of GQD. These data indicate potential usefulness of GQD in photodynamic therapy, but also raise concerns about their possible toxicity. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Biomaterials",
title = "Graphene quantum dots as autophagy-inducing photodynamic agents",
volume = "33",
number = "29",
pages = "7084-7092",
doi = "10.1016/j.biomaterials.2012.06.060"
}

Marković, Z. M., Ristić, B. Z., Arsikin, K. M., Klisic, D. G., Harhaji-Trajković, L. M., Todorović-Marković, B., Kepić, D. P., Kravić-Stevović, T. K., Jovanović, S. P., Milenković, M., Milivojević, D., Bumbaširević, V. Ž., Dramićanin, M.,& Trajković, V. S.. (2012). Graphene quantum dots as autophagy-inducing photodynamic agents. in Biomaterials, 33(29), 7084-7092.
https://doi.org/10.1016/j.biomaterials.2012.06.060

Marković ZM, Ristić BZ, Arsikin KM, Klisic DG, Harhaji-Trajković LM, Todorović-Marković B, Kepić DP, Kravić-Stevović TK, Jovanović SP, Milenković M, Milivojević D, Bumbaširević VŽ, Dramićanin M, Trajković VS. Graphene quantum dots as autophagy-inducing photodynamic agents. in Biomaterials. 2012;33(29):7084-7092.
doi:10.1016/j.biomaterials.2012.06.060 .

Marković, Zoran M., Ristić, Biljana Z., Arsikin, Katarina M., Klisic, Djordje G., Harhaji-Trajković, Ljubica M., Todorović-Marković, Biljana, Kepić, Dejan P., Kravić-Stevović, Tamara K., Jovanović, Svetlana P., Milenković, Marina, Milivojević, Dušan, Bumbaširević, Vladimir Ž., Dramićanin, Miroslav, Trajković, Vladimir S., "Graphene quantum dots as autophagy-inducing photodynamic agents" in Biomaterials, 33, no. 29 (2012):7084-7092,
https://doi.org/10.1016/j.biomaterials.2012.06.060 . .