TY  - JOUR
AU  - Jović, Danica
AU  - Jaćević, Vesna
AU  - Kuča, Kamil
AU  - Borišev, Ivana
AU  - Mrđanović, Jasminka
AU  - Petrović, Danijela
AU  - Seke, Mariana
AU  - Đorđević, Aleksandar
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9120
AB  - Being a member of the nanofamily, carbon nanomaterials exhibit specific properties that mostly arise from their small size. They have proved to be very promising for application in the technical and biomedical field. A wide spectrum of use implies the inevitable presence of carbon nanomaterials in the environment, thus potentially endangering their whole nature. Although scientists worldwide have conducted research investigating the impact of these materials, it is evident that there are still significant gaps concerning the knowledge of their mechanisms, as well as the prolonged and chronic exposure and effects. This manuscript summarizes the most prominent representatives of carbon nanomaterial groups, giving a brief review of their general physico-chemical properties, the most common use, and toxicity profiles. Toxicity was presented through genotoxicity and the activation of the cell signaling pathways, both including in vitro and in vivo models, mechanisms, and the consequential outcomes. Moreover, the acute toxicity of fullerenol, as one of the most commonly investigated members, was briefly presented in the final part of this review. Thinking small can greatly help us improve our lives, but also obliges us to deeply and comprehensively investigate all the possible consequences that could arise from our pure-hearted scientific ambitions and work.
T2  - Nanomaterials
T1  - The Puzzling Potential of Carbon Nanomaterials: General Properties, Application, and Toxicity
VL  - 10
IS  - 8
SP  - 1508
DO  - 10.3390/nano10081508
ER  - 

@article{
author = "Jović, Danica and Jaćević, Vesna and Kuča, Kamil and Borišev, Ivana and Mrđanović, Jasminka and Petrović, Danijela and Seke, Mariana and Đorđević, Aleksandar",
year = "2020",
abstract = "Being a member of the nanofamily, carbon nanomaterials exhibit specific properties that mostly arise from their small size. They have proved to be very promising for application in the technical and biomedical field. A wide spectrum of use implies the inevitable presence of carbon nanomaterials in the environment, thus potentially endangering their whole nature. Although scientists worldwide have conducted research investigating the impact of these materials, it is evident that there are still significant gaps concerning the knowledge of their mechanisms, as well as the prolonged and chronic exposure and effects. This manuscript summarizes the most prominent representatives of carbon nanomaterial groups, giving a brief review of their general physico-chemical properties, the most common use, and toxicity profiles. Toxicity was presented through genotoxicity and the activation of the cell signaling pathways, both including in vitro and in vivo models, mechanisms, and the consequential outcomes. Moreover, the acute toxicity of fullerenol, as one of the most commonly investigated members, was briefly presented in the final part of this review. Thinking small can greatly help us improve our lives, but also obliges us to deeply and comprehensively investigate all the possible consequences that could arise from our pure-hearted scientific ambitions and work.",
journal = "Nanomaterials",
title = "The Puzzling Potential of Carbon Nanomaterials: General Properties, Application, and Toxicity",
volume = "10",
number = "8",
pages = "1508",
doi = "10.3390/nano10081508"
}

Jović, D., Jaćević, V., Kuča, K., Borišev, I., Mrđanović, J., Petrović, D., Seke, M.,& Đorđević, A.. (2020). The Puzzling Potential of Carbon Nanomaterials: General Properties, Application, and Toxicity. in Nanomaterials, 10(8), 1508.
https://doi.org/10.3390/nano10081508

Jović D, Jaćević V, Kuča K, Borišev I, Mrđanović J, Petrović D, Seke M, Đorđević A. The Puzzling Potential of Carbon Nanomaterials: General Properties, Application, and Toxicity. in Nanomaterials. 2020;10(8):1508.
doi:10.3390/nano10081508 .

Jović, Danica, Jaćević, Vesna, Kuča, Kamil, Borišev, Ivana, Mrđanović, Jasminka, Petrović, Danijela, Seke, Mariana, Đorđević, Aleksandar, "The Puzzling Potential of Carbon Nanomaterials: General Properties, Application, and Toxicity" in Nanomaterials, 10, no. 8 (2020):1508,
https://doi.org/10.3390/nano10081508 . .

TY  - JOUR
AU  - Petrović, Danijela
AU  - Seke, Mariana
AU  - Labudović-Borović, Milica
AU  - Jović, Danica S.
AU  - Borišev, Ivana
AU  - Srđenović, Branislava U.
AU  - Rakočević, Zlatko Lj.
AU  - Pavlović, Vladimir B.
AU  - Đorđević, Aleksandar N.
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0014480017305890
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7688
AB  - In our recent studies we have designed fullerenol/doxorubicin nanocomposite (FNP/DOX) as the new drug nanocarrier. This research has demonstrated that this novel nanocomposite has had better implications on the liver tissue in vivo (Wistar rats treated intraperitoneally), than treatment based only on DOX. FNP/DOX has been characterised by DLS, TEM and AFM measurements which have shown that DOX loaded onto FNP did not influence fullerenol nanoparticle's size. FNP/DOX affected oxidative status in blood causing a significant decrease of catalase and SOD activity in comparison to DOX, implicating the reduction in oxidative stress. qRT-PCR results on the mRNA level of antioxidative enzymes (catalase and MnSOD) revealed that the effect of oxidative stress is significantly reduced by the treatment with FNP/DOX (p <.05). The ultrastructural analysis of the liver tissue has revealed that FNP/DOX nanocomposite generated considerably less damage in the liver tissue, than DOX applied at the same dose. Hence, our results have indicated that FNP, within FNP/DOX nanocomposite, exhibits protective effects to the liver tissue of the healthy rats.
T2  - Experimental and Molecular Pathology
T1  - Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats
VL  - 104
IS  - 3
SP  - 199
EP  - 211
DO  - 10.1016/j.yexmp.2018.04.005
ER  - 

@article{
author = "Petrović, Danijela and Seke, Mariana and Labudović-Borović, Milica and Jović, Danica S. and Borišev, Ivana and Srđenović, Branislava U. and Rakočević, Zlatko Lj. and Pavlović, Vladimir B. and Đorđević, Aleksandar N.",
year = "2018",
abstract = "In our recent studies we have designed fullerenol/doxorubicin nanocomposite (FNP/DOX) as the new drug nanocarrier. This research has demonstrated that this novel nanocomposite has had better implications on the liver tissue in vivo (Wistar rats treated intraperitoneally), than treatment based only on DOX. FNP/DOX has been characterised by DLS, TEM and AFM measurements which have shown that DOX loaded onto FNP did not influence fullerenol nanoparticle's size. FNP/DOX affected oxidative status in blood causing a significant decrease of catalase and SOD activity in comparison to DOX, implicating the reduction in oxidative stress. qRT-PCR results on the mRNA level of antioxidative enzymes (catalase and MnSOD) revealed that the effect of oxidative stress is significantly reduced by the treatment with FNP/DOX (p <.05). The ultrastructural analysis of the liver tissue has revealed that FNP/DOX nanocomposite generated considerably less damage in the liver tissue, than DOX applied at the same dose. Hence, our results have indicated that FNP, within FNP/DOX nanocomposite, exhibits protective effects to the liver tissue of the healthy rats.",
journal = "Experimental and Molecular Pathology",
title = "Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats",
volume = "104",
number = "3",
pages = "199-211",
doi = "10.1016/j.yexmp.2018.04.005"
}

Petrović, D., Seke, M., Labudović-Borović, M., Jović, D. S., Borišev, I., Srđenović, B. U., Rakočević, Z. Lj., Pavlović, V. B.,& Đorđević, A. N.. (2018). Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats. in Experimental and Molecular Pathology, 104(3), 199-211.
https://doi.org/10.1016/j.yexmp.2018.04.005

Petrović D, Seke M, Labudović-Borović M, Jović DS, Borišev I, Srđenović BU, Rakočević ZL, Pavlović VB, Đorđević AN. Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats. in Experimental and Molecular Pathology. 2018;104(3):199-211.
doi:10.1016/j.yexmp.2018.04.005 .

Petrović, Danijela, Seke, Mariana, Labudović-Borović, Milica, Jović, Danica S., Borišev, Ivana, Srđenović, Branislava U., Rakočević, Zlatko Lj., Pavlović, Vladimir B., Đorđević, Aleksandar N., "Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats" in Experimental and Molecular Pathology, 104, no. 3 (2018):199-211,
https://doi.org/10.1016/j.yexmp.2018.04.005 . .

TY  - JOUR
AU  - Borišev, Ivana
AU  - Mrđanović, Jasminka Ž.
AU  - Petrović, Danijela
AU  - Seke, Mariana
AU  - Jović, Danica S.
AU  - Srđenović, Branislava U.
AU  - Latinović, Nataša
AU  - Đorđević, Aleksandar N.
PY  - 2018
UR  - http://stacks.iop.org/0957-4484/29/i=33/a=332002?key=crossref.4804877570e2609bf6333877ee495ab3
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7751
AB  - Nanotechnology, focused on discovery and development of new pharmaceutical products is known as nanopharmacology, and one research area this branch is engaged in are nanopharmaceuticals. The importance of being nano has been particularly emphasized in scientific areas dealing with nanomedicine and nanopharmaceuticals. Nanopharmaceuticals, their routes of administration, obstacles and solutions concerning their improved application and enhanced efficacy have been briefly yet comprehensively described. Cancer is one of the leading causes of death worldwide and evergrowing number of scientific research on the topic only confirms that the needs have not been completed yet and that there is a wide platform for improvement. This is undoubtedly true for nanoformulations of an anticancer drug doxorubicin, where various nanocarrriers were given an important role to reduce the drug toxicity, while the efficacy of the drug was supposed to be retained or preferably enhanced. Therefore, we present an interdisciplinary comprehensive overview of interdisciplinary nature on nanopharmaceuticals based on doxorubicin and its nanoformulations with valuable information concerning trends, obstacles and prospective of nanopharmaceuticals development, mode of activity of sole drug doxorubicin and its nanoformulations based on different nanocarriers, their brief descriptions of biological activity through assessing in vitro and in vivo behavior.
T2  - Nanotechnology
T1  - Nanoformulations of doxorubicin: How far have we come and where do we go from here?
VL  - 29
IS  - 33
SP  - 332002
DO  - 10.1088/1361-6528/aac7dd
ER  - 

@article{
author = "Borišev, Ivana and Mrđanović, Jasminka Ž. and Petrović, Danijela and Seke, Mariana and Jović, Danica S. and Srđenović, Branislava U. and Latinović, Nataša and Đorđević, Aleksandar N.",
year = "2018",
abstract = "Nanotechnology, focused on discovery and development of new pharmaceutical products is known as nanopharmacology, and one research area this branch is engaged in are nanopharmaceuticals. The importance of being nano has been particularly emphasized in scientific areas dealing with nanomedicine and nanopharmaceuticals. Nanopharmaceuticals, their routes of administration, obstacles and solutions concerning their improved application and enhanced efficacy have been briefly yet comprehensively described. Cancer is one of the leading causes of death worldwide and evergrowing number of scientific research on the topic only confirms that the needs have not been completed yet and that there is a wide platform for improvement. This is undoubtedly true for nanoformulations of an anticancer drug doxorubicin, where various nanocarrriers were given an important role to reduce the drug toxicity, while the efficacy of the drug was supposed to be retained or preferably enhanced. Therefore, we present an interdisciplinary comprehensive overview of interdisciplinary nature on nanopharmaceuticals based on doxorubicin and its nanoformulations with valuable information concerning trends, obstacles and prospective of nanopharmaceuticals development, mode of activity of sole drug doxorubicin and its nanoformulations based on different nanocarriers, their brief descriptions of biological activity through assessing in vitro and in vivo behavior.",
journal = "Nanotechnology",
title = "Nanoformulations of doxorubicin: How far have we come and where do we go from here?",
volume = "29",
number = "33",
pages = "332002",
doi = "10.1088/1361-6528/aac7dd"
}

Borišev, I., Mrđanović, J. Ž., Petrović, D., Seke, M., Jović, D. S., Srđenović, B. U., Latinović, N.,& Đorđević, A. N.. (2018). Nanoformulations of doxorubicin: How far have we come and where do we go from here?. in Nanotechnology, 29(33), 332002.
https://doi.org/10.1088/1361-6528/aac7dd

Borišev I, Mrđanović JŽ, Petrović D, Seke M, Jović DS, Srđenović BU, Latinović N, Đorđević AN. Nanoformulations of doxorubicin: How far have we come and where do we go from here?. in Nanotechnology. 2018;29(33):332002.
doi:10.1088/1361-6528/aac7dd .

Borišev, Ivana, Mrđanović, Jasminka Ž., Petrović, Danijela, Seke, Mariana, Jović, Danica S., Srđenović, Branislava U., Latinović, Nataša, Đorđević, Aleksandar N., "Nanoformulations of doxorubicin: How far have we come and where do we go from here?" in Nanotechnology, 29, no. 33 (2018):332002,
https://doi.org/10.1088/1361-6528/aac7dd . .

TY  - CONF
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Labudović-Borović, Milica
AU  - Jović, Danica S.
AU  - Borišev, Ivana
AU  - Kanacki, Zdenko
AU  - Zikić, Dragan
AU  - Đorđević, Aleksandar N.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7182
AB  - Background: Doxorubicin is a first line cancer chemotherapeutic. Unfortunately, its clinical use is limited by its cardiotoxicity. It is known that iron overload aggravates anthracycline toxicity. Fullerenol is a 1 nm size molecule and in aqueous solutions is in the form of polyanionic nanoparticles, which enables them to serve as a good carrier of positively charged ions such as Fe2+. Fullerenol’s antioxidant activity through scavenging free radicals has already been proved in different biological systems.
Methods: The aim of our study was to investigate the effects of the fullerenol/iron nanocomposite as a pretreatment to doxorubicin on the rat’s heart in comparison to doxorubicin alone. After the 24h-treatment, adult male Wistar rats were sacrificed and hearts were collected for ultrastructural and qRT-PCR analysis. Considering the ability of doxorubicin to induce oxidative stress, and the fullerenol’s capability to mitigate it, we had chosen to monitor gene expression of enzymes involved in antioxidant defense.
Results: Ultrastructural study revealed that in the group pretreated with the nanocomposite prior to doxorubicin application cardiomyocytes were with preserved morphology and the structure of intercalated discs. On the other hand, the heart tissues of animals treated with doxorubicin alone were significantly more damaged. Intensive interstitial edema was observed, as well as vacuolization of cardiomyocytes, hypercontraction of sarcomeres, mitochondria of irregular shapes. qRT-PCR results have shown that neither treatment with doxorubicin alone nor the pretreatment with the nanocomposite did cause significant increase in mRNA levels of catalase and superoxide dismutase.
Conclusions: Our results indicate that the fullerenol/iron nanocomposite applied as pretreatment to doxorubicin induces less damage to the hearth tissue in comparison to doxorubicin alone.
C3  - Annals of Oncology
T1  - Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity
VL  - 28
IS  - Supplement 5
DO  - 10.1093/annonc/mdx390.057
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7182
ER  - 

@conference{
author = "Seke, Mariana and Petrović, Danijela and Labudović-Borović, Milica and Jović, Danica S. and Borišev, Ivana and Kanacki, Zdenko and Zikić, Dragan and Đorđević, Aleksandar N.",
year = "2017",
abstract = "Background: Doxorubicin is a first line cancer chemotherapeutic. Unfortunately, its clinical use is limited by its cardiotoxicity. It is known that iron overload aggravates anthracycline toxicity. Fullerenol is a 1 nm size molecule and in aqueous solutions is in the form of polyanionic nanoparticles, which enables them to serve as a good carrier of positively charged ions such as Fe2+. Fullerenol’s antioxidant activity through scavenging free radicals has already been proved in different biological systems.
Methods: The aim of our study was to investigate the effects of the fullerenol/iron nanocomposite as a pretreatment to doxorubicin on the rat’s heart in comparison to doxorubicin alone. After the 24h-treatment, adult male Wistar rats were sacrificed and hearts were collected for ultrastructural and qRT-PCR analysis. Considering the ability of doxorubicin to induce oxidative stress, and the fullerenol’s capability to mitigate it, we had chosen to monitor gene expression of enzymes involved in antioxidant defense.
Results: Ultrastructural study revealed that in the group pretreated with the nanocomposite prior to doxorubicin application cardiomyocytes were with preserved morphology and the structure of intercalated discs. On the other hand, the heart tissues of animals treated with doxorubicin alone were significantly more damaged. Intensive interstitial edema was observed, as well as vacuolization of cardiomyocytes, hypercontraction of sarcomeres, mitochondria of irregular shapes. qRT-PCR results have shown that neither treatment with doxorubicin alone nor the pretreatment with the nanocomposite did cause significant increase in mRNA levels of catalase and superoxide dismutase.
Conclusions: Our results indicate that the fullerenol/iron nanocomposite applied as pretreatment to doxorubicin induces less damage to the hearth tissue in comparison to doxorubicin alone.",
journal = "Annals of Oncology",
title = "Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity",
volume = "28",
number = "Supplement 5",
doi = "10.1093/annonc/mdx390.057",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7182"
}

Seke, M., Petrović, D., Labudović-Borović, M., Jović, D. S., Borišev, I., Kanacki, Z., Zikić, D.,& Đorđević, A. N.. (2017). Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity. in Annals of Oncology, 28(Supplement 5).
https://doi.org/10.1093/annonc/mdx390.057
https://hdl.handle.net/21.15107/rcub_vinar_7182

Seke M, Petrović D, Labudović-Borović M, Jović DS, Borišev I, Kanacki Z, Zikić D, Đorđević AN. Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity. in Annals of Oncology. 2017;28(Supplement 5).
doi:10.1093/annonc/mdx390.057
https://hdl.handle.net/21.15107/rcub_vinar_7182 .

Seke, Mariana, Petrović, Danijela, Labudović-Borović, Milica, Jović, Danica S., Borišev, Ivana, Kanacki, Zdenko, Zikić, Dragan, Đorđević, Aleksandar N., "Fullerenol/iron nanocomposite modulates doxorubicin-induced cardiotoxicity" in Annals of Oncology, 28, no. Supplement 5 (2017),
https://doi.org/10.1093/annonc/mdx390.057 .,
https://hdl.handle.net/21.15107/rcub_vinar_7182 .