Milanović, Zorana

Link to this page

Authority KeyName Variants
orcid::0000-0001-9953-3269
  • Milanović, Zorana (29)
Projects
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) Magnetic and radionuclide labeled nanostructured materials for medical applications
EUREKA [E! 13303 MED-BIO-TEST] Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200146 (University of Belgrade, Faculty of Physical Chemistry) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry)
COST Action CA [19114] COST Action RADIOMAG [TD1402]
ERDF [“BIOVID-19”] ERDF [ITMS 313011AVG3]
Grant Agency of the Slovak Republic VEGA [1/0159/20] Rational design and synthesis of biologically active and coordination compounds and functional materials, relevant for (bio)nanotechnology
Antioxidative defense, differentiation and regeneration potential of tissue specific mesenchymal stem cells during ageing Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200162 (University of Belgrade, Faculty of Physics)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200288 (Innovation Center of the Faculty of Chemistry) Studying climate change and its influence on environment: impacts, adaptation and mitigation
Advanced technologies for monitoring and environmental protection from chemical pollutants and radiation burden Inovacioni fond Republike Srbije u okviru Programa Dokaz koncepta, projekat PoC5628
Inovacioni fond Republike Srbije u okviru Programa Transfer tehnologiija TT1118 Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije (451-03-68/2020-14)
Ministry of Education, Science and Technological Development of the Republic of Serbia Operational Programme Integrated Infrastructure [ITMS: 313011AUW7]
Operational Programme Integrated Infrastructure [“NANOVIR”] Slovak Research and Development Agency [APVV-14-0120]
Slovak Research and Development Agency [APVV-DS-FR-19-0052] Slovak Research and Development Agency [APVV-SK-SRB-18-0055]
The VINCENT Center of Excellence.

Author's Bibliography

Optimization of radioprotective dose of Juglans nigra leaf extract using response surface methodology

Rajković, Katarina M.; Đurašević, Mirjana; Markićević, Milan; Milanović, Zorana; Vranješ-Đurić, Sanja; Janković, Drina; Stanković, Dragana; Obradović, Zorica

(2024)

TY  - JOUR
AU  - Rajković, Katarina M.
AU  - Đurašević, Mirjana
AU  - Markićević, Milan
AU  - Milanović, Zorana
AU  - Vranješ-Đurić, Sanja
AU  - Janković, Drina
AU  - Stanković, Dragana
AU  - Obradović, Zorica
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12239
AB  - J. nigra leaf contains mixture of various pharmacologically active compounds and it is assumed that they may have the potential radioprotective effect. The purpose of this work was to predict radioprotective doses by correlating changes in organ distribution of radiopharmaceuticals with extract dose levels and rat body weight using response surface methodology (RSM) based on a second-order polynomial equation. The extract was administered daily by oral gavage to rats at dose of 6.9, 10.3, or 13.7 mg kg−1 body weight (bw) day−1 for 10 days. On the eleventh day, 0.1 ml of the one radiopharmaceutical (Na99mTcO4, 99mTc-dimercaptosuccinic acid and 99mTc-tin colloid) was injected into the tail vein. The statistical parameters: the coefficient of determination (0.81–0.95), the coefficient of variation (3.05–11.1), the adequate precision (8.82–19.12) and the mean relative percentage deviation (± 2.3–8.2) were indicated the precision and reliability of RSM. Using RSM, the predicted daily dose of leaf extract ranging from 11.19 to 11.99 mg kg−1 bw may be considered as an optimal daily radiopotective dose for protection of organs from radiation in cases of planned radiation exposures.
T2  - Journal of Environmental Radioactivity
T1  - Optimization of radioprotective dose of Juglans nigra leaf extract using response surface methodology
VL  - 272
SP  - 107333
DO  - 10.1016/j.jenvrad.2023.107333
ER  - 
@article{
author = "Rajković, Katarina M. and Đurašević, Mirjana and Markićević, Milan and Milanović, Zorana and Vranješ-Đurić, Sanja and Janković, Drina and Stanković, Dragana and Obradović, Zorica",
year = "2024",
abstract = "J. nigra leaf contains mixture of various pharmacologically active compounds and it is assumed that they may have the potential radioprotective effect. The purpose of this work was to predict radioprotective doses by correlating changes in organ distribution of radiopharmaceuticals with extract dose levels and rat body weight using response surface methodology (RSM) based on a second-order polynomial equation. The extract was administered daily by oral gavage to rats at dose of 6.9, 10.3, or 13.7 mg kg−1 body weight (bw) day−1 for 10 days. On the eleventh day, 0.1 ml of the one radiopharmaceutical (Na99mTcO4, 99mTc-dimercaptosuccinic acid and 99mTc-tin colloid) was injected into the tail vein. The statistical parameters: the coefficient of determination (0.81–0.95), the coefficient of variation (3.05–11.1), the adequate precision (8.82–19.12) and the mean relative percentage deviation (± 2.3–8.2) were indicated the precision and reliability of RSM. Using RSM, the predicted daily dose of leaf extract ranging from 11.19 to 11.99 mg kg−1 bw may be considered as an optimal daily radiopotective dose for protection of organs from radiation in cases of planned radiation exposures.",
journal = "Journal of Environmental Radioactivity",
title = "Optimization of radioprotective dose of Juglans nigra leaf extract using response surface methodology",
volume = "272",
pages = "107333",
doi = "10.1016/j.jenvrad.2023.107333"
}
Rajković, K. M., Đurašević, M., Markićević, M., Milanović, Z., Vranješ-Đurić, S., Janković, D., Stanković, D.,& Obradović, Z.. (2024). Optimization of radioprotective dose of Juglans nigra leaf extract using response surface methodology. in Journal of Environmental Radioactivity, 272, 107333.
https://doi.org/10.1016/j.jenvrad.2023.107333
Rajković KM, Đurašević M, Markićević M, Milanović Z, Vranješ-Đurić S, Janković D, Stanković D, Obradović Z. Optimization of radioprotective dose of Juglans nigra leaf extract using response surface methodology. in Journal of Environmental Radioactivity. 2024;272:107333.
doi:10.1016/j.jenvrad.2023.107333 .
Rajković, Katarina M., Đurašević, Mirjana, Markićević, Milan, Milanović, Zorana, Vranješ-Đurić, Sanja, Janković, Drina, Stanković, Dragana, Obradović, Zorica, "Optimization of radioprotective dose of Juglans nigra leaf extract using response surface methodology" in Journal of Environmental Radioactivity, 272 (2024):107333,
https://doi.org/10.1016/j.jenvrad.2023.107333 . .

Synthesis, Characterization, and Therapeutic Efficacy of 177Lu-DMSA@SPIONs in Nanobrachytherapy of Solid Tumors

Stanković, Dragana; Radović, Magdalena; Stanković, Aljoša; Mirković, Marija; Vukadinović, Aleksandar; Mijović, Milica; Milanović, Zorana; Ognjanović, Miloš; Janković, Drina; Antić, Bratislav; Vranješ-Đurić, Sanja; Savić, Miroslav; Prijović, Željko

(2023)

TY  - JOUR
AU  - Stanković, Dragana
AU  - Radović, Magdalena
AU  - Stanković, Aljoša
AU  - Mirković, Marija
AU  - Vukadinović, Aleksandar
AU  - Mijović, Milica
AU  - Milanović, Zorana
AU  - Ognjanović, Miloš
AU  - Janković, Drina
AU  - Antić, Bratislav
AU  - Vranješ-Đurić, Sanja
AU  - Savić, Miroslav
AU  - Prijović, Željko
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11387
AB  - As an alternative to classical brachytherapy, intratumoral injection of radionuclide-labeled nanoparticles (nanobrachytherapy, NBT) has been investigated as a superior delivery method over an intravenous route for radionuclide therapy of solid tumors. We created superparamagnetic iron oxide nanoparticles (SPIONs) coated with meso-1,2-dimercaptosuccinic acid (DMSA) and radiolabeled with Lutetium-177 (177Lu), generating 177Lu-DMSA@SPIONs as a potential antitumor agent for nanobrachytherapy. Efficient radiolabeling of DMSA@SPIONS by 177Lu resulted in a stable bond with minimal leakage in vitro. After an intratumoral injection to mouse colorectal CT-26 or breast 4T1 subcutaneous tumors, the nanoparticles remained well localized at the injection site for weeks, with limited leakage. The dose of 3.70 MBq/100 µg/50 µL of 177Lu-DMSA@SPIONs applied intratumorally resulted in a high therapeutic efficacy, without signs of general toxicity. A decreased dose of 1.85 MBq/100 µg/50 µL still retained therapeutic efficacy, while an increased dose of 9.25 MBq/100 µg/50 µL did not significantly benefit the therapy. Histopathology analysis revealed that the 177Lu-DMSA@SPIONs act within a limited range around the injection site, which explains the good therapeutic efficacy achieved by a single administration of a relatively low dose without the need for increased or repeated dosing. Overall, 177Lu-DMSA@SPIONs are safe and potent agents suitable for intra-tumoral administration for localized tumor radionuclide therapy
T2  - Pharmaceutics
T1  - Synthesis, Characterization, and Therapeutic Efficacy of 177Lu-DMSA@SPIONs in Nanobrachytherapy of Solid Tumors
VL  - 15
IS  - 7
SP  - 1943
DO  - 10.3390/pharmaceutics15071943
ER  - 
@article{
author = "Stanković, Dragana and Radović, Magdalena and Stanković, Aljoša and Mirković, Marija and Vukadinović, Aleksandar and Mijović, Milica and Milanović, Zorana and Ognjanović, Miloš and Janković, Drina and Antić, Bratislav and Vranješ-Đurić, Sanja and Savić, Miroslav and Prijović, Željko",
year = "2023",
abstract = "As an alternative to classical brachytherapy, intratumoral injection of radionuclide-labeled nanoparticles (nanobrachytherapy, NBT) has been investigated as a superior delivery method over an intravenous route for radionuclide therapy of solid tumors. We created superparamagnetic iron oxide nanoparticles (SPIONs) coated with meso-1,2-dimercaptosuccinic acid (DMSA) and radiolabeled with Lutetium-177 (177Lu), generating 177Lu-DMSA@SPIONs as a potential antitumor agent for nanobrachytherapy. Efficient radiolabeling of DMSA@SPIONS by 177Lu resulted in a stable bond with minimal leakage in vitro. After an intratumoral injection to mouse colorectal CT-26 or breast 4T1 subcutaneous tumors, the nanoparticles remained well localized at the injection site for weeks, with limited leakage. The dose of 3.70 MBq/100 µg/50 µL of 177Lu-DMSA@SPIONs applied intratumorally resulted in a high therapeutic efficacy, without signs of general toxicity. A decreased dose of 1.85 MBq/100 µg/50 µL still retained therapeutic efficacy, while an increased dose of 9.25 MBq/100 µg/50 µL did not significantly benefit the therapy. Histopathology analysis revealed that the 177Lu-DMSA@SPIONs act within a limited range around the injection site, which explains the good therapeutic efficacy achieved by a single administration of a relatively low dose without the need for increased or repeated dosing. Overall, 177Lu-DMSA@SPIONs are safe and potent agents suitable for intra-tumoral administration for localized tumor radionuclide therapy",
journal = "Pharmaceutics",
title = "Synthesis, Characterization, and Therapeutic Efficacy of 177Lu-DMSA@SPIONs in Nanobrachytherapy of Solid Tumors",
volume = "15",
number = "7",
pages = "1943",
doi = "10.3390/pharmaceutics15071943"
}
Stanković, D., Radović, M., Stanković, A., Mirković, M., Vukadinović, A., Mijović, M., Milanović, Z., Ognjanović, M., Janković, D., Antić, B., Vranješ-Đurić, S., Savić, M.,& Prijović, Ž.. (2023). Synthesis, Characterization, and Therapeutic Efficacy of 177Lu-DMSA@SPIONs in Nanobrachytherapy of Solid Tumors. in Pharmaceutics, 15(7), 1943.
https://doi.org/10.3390/pharmaceutics15071943
Stanković D, Radović M, Stanković A, Mirković M, Vukadinović A, Mijović M, Milanović Z, Ognjanović M, Janković D, Antić B, Vranješ-Đurić S, Savić M, Prijović Ž. Synthesis, Characterization, and Therapeutic Efficacy of 177Lu-DMSA@SPIONs in Nanobrachytherapy of Solid Tumors. in Pharmaceutics. 2023;15(7):1943.
doi:10.3390/pharmaceutics15071943 .
Stanković, Dragana, Radović, Magdalena, Stanković, Aljoša, Mirković, Marija, Vukadinović, Aleksandar, Mijović, Milica, Milanović, Zorana, Ognjanović, Miloš, Janković, Drina, Antić, Bratislav, Vranješ-Đurić, Sanja, Savić, Miroslav, Prijović, Željko, "Synthesis, Characterization, and Therapeutic Efficacy of 177Lu-DMSA@SPIONs in Nanobrachytherapy of Solid Tumors" in Pharmaceutics, 15, no. 7 (2023):1943,
https://doi.org/10.3390/pharmaceutics15071943 . .
1

The prediction of radioprotective dose of a Juglansnigra L. leaf extracts in diagnostic irradiation using response surface methodology

Đurašević, Mirjana M.; Rajković, Katarina; Milanović, Zorana; Vranješ-Đurić, Sanja; Janković, Drina; Mirković, Marija D.; Obradović, Zorica

(Niš : RAD Centre, 2023)

TY  - CONF
AU  - Đurašević, Mirjana M.
AU  - Rajković, Katarina
AU  - Milanović, Zorana
AU  - Vranješ-Đurić, Sanja
AU  - Janković, Drina
AU  - Mirković, Marija D.
AU  - Obradović, Zorica
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12232
AB  - Juglansnigraleaf is a source of health-promoting biologically active compounds and used in traditional
medicine. Can we predict theradioprotective dose level of plant extracts based on modeling change
biodistribution of radiopharmaceuticals by response surface methodology (RSM)? The present study aimed
to the effects of J. nigra leaf extract oral intake on the biodistribution of 99mTc-radiopharmaceutical in
healthy rats and to formulate a mathematical model that will associate the changes in radioactivity in organ
with dose levels of extract and body mass of rats. The extract was administered daily by oral gavage to rats at
dose levels of 6.9, 10.3, or 13.7 mg kg-1 body weight (bw) day-1 for 10 days. On the eleventh day, 0.1 ml
(approximately 148 kBq) of the 99mTc-dimercaptosuccinic acid (DMSA) was injected into the tail vein. The
organs of interest were isolated and radioactivity in each organ was counted by a gamma counter with a NaI
(Tl) detector. After treatment of rats with the extract, there was a statistically significant decrease (p<0.05) in
the uptake of 99mTc-DMSA (%ID/organ) in the kidneys compared to controls.The RSM model based on the
second-order polynomial equation was apply to correlate changes in radioactivity in kidney with dose levels of
extract and body mass of rats.The statistical significance of RSM as well as independent variables and their
interactions were estimated by ANOVA (Analysis of variance). The F-value (265) and p-value (< 0,001)
demonstrated that the developed model has statistical significance at the confidence level of 95%. The R2
values (0.987) proved a good fit by the second-order polynomial equation, while relatively low values of the
CV (0.69) indicated the remarkable precision and reliability of the model. The low value of mean relative
percent deviation (0.4) between the experimental data and the predicted radioactivity of kidney obtained by
RSM showed that RSM was suitable for modeling the change biodistributionof radiopharmaceutical.
Modeling data provided evidence that the radioactivityof kidney statistically depended dose levels of extract
and statistically not depended of body mass of rats. RSM gave the minimum predicted value of radioactivity
of kidney 59%, which corresponded to minimum dose levelof 11.18 mg kg-1and minimum body massof 130 g.
RSM showed that extracts of J. nigraleaf kidney system protectionat doses higher than 11.18 mg kg-1. These
results suggest the potential use minimal dose level of J. nigraleaf extract as a radioprotector in cases of
planned radiation exposures.
PB  - Niš : RAD Centre
C3  - RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts
T1  - The prediction of radioprotective dose of a Juglansnigra L. leaf extracts in diagnostic irradiation using response surface methodology
SP  - 265
EP  - 265
DO  - 10.21175/rad.abstr.book.2023.41.1
ER  - 
@conference{
author = "Đurašević, Mirjana M. and Rajković, Katarina and Milanović, Zorana and Vranješ-Đurić, Sanja and Janković, Drina and Mirković, Marija D. and Obradović, Zorica",
year = "2023",
abstract = "Juglansnigraleaf is a source of health-promoting biologically active compounds and used in traditional
medicine. Can we predict theradioprotective dose level of plant extracts based on modeling change
biodistribution of radiopharmaceuticals by response surface methodology (RSM)? The present study aimed
to the effects of J. nigra leaf extract oral intake on the biodistribution of 99mTc-radiopharmaceutical in
healthy rats and to formulate a mathematical model that will associate the changes in radioactivity in organ
with dose levels of extract and body mass of rats. The extract was administered daily by oral gavage to rats at
dose levels of 6.9, 10.3, or 13.7 mg kg-1 body weight (bw) day-1 for 10 days. On the eleventh day, 0.1 ml
(approximately 148 kBq) of the 99mTc-dimercaptosuccinic acid (DMSA) was injected into the tail vein. The
organs of interest were isolated and radioactivity in each organ was counted by a gamma counter with a NaI
(Tl) detector. After treatment of rats with the extract, there was a statistically significant decrease (p<0.05) in
the uptake of 99mTc-DMSA (%ID/organ) in the kidneys compared to controls.The RSM model based on the
second-order polynomial equation was apply to correlate changes in radioactivity in kidney with dose levels of
extract and body mass of rats.The statistical significance of RSM as well as independent variables and their
interactions were estimated by ANOVA (Analysis of variance). The F-value (265) and p-value (< 0,001)
demonstrated that the developed model has statistical significance at the confidence level of 95%. The R2
values (0.987) proved a good fit by the second-order polynomial equation, while relatively low values of the
CV (0.69) indicated the remarkable precision and reliability of the model. The low value of mean relative
percent deviation (0.4) between the experimental data and the predicted radioactivity of kidney obtained by
RSM showed that RSM was suitable for modeling the change biodistributionof radiopharmaceutical.
Modeling data provided evidence that the radioactivityof kidney statistically depended dose levels of extract
and statistically not depended of body mass of rats. RSM gave the minimum predicted value of radioactivity
of kidney 59%, which corresponded to minimum dose levelof 11.18 mg kg-1and minimum body massof 130 g.
RSM showed that extracts of J. nigraleaf kidney system protectionat doses higher than 11.18 mg kg-1. These
results suggest the potential use minimal dose level of J. nigraleaf extract as a radioprotector in cases of
planned radiation exposures.",
publisher = "Niš : RAD Centre",
journal = "RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts",
title = "The prediction of radioprotective dose of a Juglansnigra L. leaf extracts in diagnostic irradiation using response surface methodology",
pages = "265-265",
doi = "10.21175/rad.abstr.book.2023.41.1"
}
Đurašević, M. M., Rajković, K., Milanović, Z., Vranješ-Đurić, S., Janković, D., Mirković, M. D.,& Obradović, Z.. (2023). The prediction of radioprotective dose of a Juglansnigra L. leaf extracts in diagnostic irradiation using response surface methodology. in RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts
Niš : RAD Centre., 265-265.
https://doi.org/10.21175/rad.abstr.book.2023.41.1
Đurašević MM, Rajković K, Milanović Z, Vranješ-Đurić S, Janković D, Mirković MD, Obradović Z. The prediction of radioprotective dose of a Juglansnigra L. leaf extracts in diagnostic irradiation using response surface methodology. in RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts. 2023;:265-265.
doi:10.21175/rad.abstr.book.2023.41.1 .
Đurašević, Mirjana M., Rajković, Katarina, Milanović, Zorana, Vranješ-Đurić, Sanja, Janković, Drina, Mirković, Marija D., Obradović, Zorica, "The prediction of radioprotective dose of a Juglansnigra L. leaf extracts in diagnostic irradiation using response surface methodology" in RAD 2023 : 11th International Conference on Radiation Natural Sciences, Medicine, Engineering, Technology and Ecology : Book of Abstracts (2023):265-265,
https://doi.org/10.21175/rad.abstr.book.2023.41.1 . .

Eksperimentalni model za procenu mogućeg radioprotektivnog efekta biljnog ekstrakta

Rajković, Katarina M.; Đurašević, Mirjana; Obradović, Zorica; Milanović, Zorana; Vukadinović, Aleksandar; Perić, Marko; Stanković, Dragana; Janković, Drina

(Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča", 2023)

TY  - CONF
AU  - Rajković, Katarina M.
AU  - Đurašević, Mirjana
AU  - Obradović, Zorica
AU  - Milanović, Zorana
AU  - Vukadinović, Aleksandar
AU  - Perić, Marko
AU  - Stanković, Dragana
AU  - Janković, Drina
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12184
AB  - Tc-99m-radiofarmaceutici se koriste u različitim dijagnostičkim procedurama u nuklearnoj medicini. Da bi se minimizirali štetni efekti jonizujućeg zračenja na zdrava tkiva, intenzivno se istražuju radioprotektivne osobine biljnih ekstrakata. Ljuska ploda crnog oraha sadrži mešavinu različitih farmakološki aktivnih jedinjenja kojа imaju antioksidativno dejstvo i za koje se pretpostavlja da mogu imati i potencijalno radioprotektivno dejstvo. U tom kontekstu, cilj rada je bio da se proceni radioprotektivni efekat ekstrakta ljuske ploda crnog oraha. U ovoj studiji primenjen je eksperimentalni model koji se zasnivao na proceni uticaja ekstrakta na biodistribuciju Tc-99m-radiofarmaceutika po organima eksperimentalnih životinja (pacovi). Ekstrakt ljuske crnog oraha doveo je do smanjenognakupljanja Tc-99m-radiofarmaceutika u pojedinim organimapacova. Rezultati dobijeni na osnovu eksperimentalnog modela sugerišu moguću upotrebu ekstrakta ljuske ploda crnog oraha kao radioprotektivnog sredstva u slučajevima planiranog izlaganja jonizujućem zračenju u nuklearnoj medicini.
AB  - Tc-99m-radiopharmaceuticals are used in various diagnostic procedures in nuclear medicine. In order to minimize the harmful effects of ionizing radiation on healthy tissues, the radioprotective properties of plant extracts are intensively researched. J. nigra husk contains a mixture of various pharmacologically active compounds that have an antioxidant effect and which are assumed to have a potential radioprotective effect. In this context, the aim of the work was to evaluate the radioprotective effect of black walnut husk extract. In this study, an experimental model was applied, which was based on the assessment of the effect of the extract on the biodistribution of Tc-99m-radiopharmaceuticals in the organs of experimental animals (rats). Black walnut husk extract led to a decrease in the accumulation of Tc-99m-radiopharmaceutical in certain organs of rats. The results obtained on the basis of the experimental model suggest that it is possible to use black walnut husk extract as a radioprotective agent in cases of planned exposure to ionizing radiation in diagnosticsnuclear medicine.
PB  - Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča"
PB  - Društvo za zaštitu od zračenja Srbije i Crne Gore
C3  - 32. simpozijum DZZSCG : zbornik radova
T1  - Eksperimentalni model za procenu mogućeg radioprotektivnog efekta biljnog ekstrakta
T1  - Experimental Model for Assessing the Possible Radioprotective Effect of Plant Extract
SP  - 321
EP  - 327
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12184
ER  - 
@conference{
author = "Rajković, Katarina M. and Đurašević, Mirjana and Obradović, Zorica and Milanović, Zorana and Vukadinović, Aleksandar and Perić, Marko and Stanković, Dragana and Janković, Drina",
year = "2023",
abstract = "Tc-99m-radiofarmaceutici se koriste u različitim dijagnostičkim procedurama u nuklearnoj medicini. Da bi se minimizirali štetni efekti jonizujućeg zračenja na zdrava tkiva, intenzivno se istražuju radioprotektivne osobine biljnih ekstrakata. Ljuska ploda crnog oraha sadrži mešavinu različitih farmakološki aktivnih jedinjenja kojа imaju antioksidativno dejstvo i za koje se pretpostavlja da mogu imati i potencijalno radioprotektivno dejstvo. U tom kontekstu, cilj rada je bio da se proceni radioprotektivni efekat ekstrakta ljuske ploda crnog oraha. U ovoj studiji primenjen je eksperimentalni model koji se zasnivao na proceni uticaja ekstrakta na biodistribuciju Tc-99m-radiofarmaceutika po organima eksperimentalnih životinja (pacovi). Ekstrakt ljuske crnog oraha doveo je do smanjenognakupljanja Tc-99m-radiofarmaceutika u pojedinim organimapacova. Rezultati dobijeni na osnovu eksperimentalnog modela sugerišu moguću upotrebu ekstrakta ljuske ploda crnog oraha kao radioprotektivnog sredstva u slučajevima planiranog izlaganja jonizujućem zračenju u nuklearnoj medicini., Tc-99m-radiopharmaceuticals are used in various diagnostic procedures in nuclear medicine. In order to minimize the harmful effects of ionizing radiation on healthy tissues, the radioprotective properties of plant extracts are intensively researched. J. nigra husk contains a mixture of various pharmacologically active compounds that have an antioxidant effect and which are assumed to have a potential radioprotective effect. In this context, the aim of the work was to evaluate the radioprotective effect of black walnut husk extract. In this study, an experimental model was applied, which was based on the assessment of the effect of the extract on the biodistribution of Tc-99m-radiopharmaceuticals in the organs of experimental animals (rats). Black walnut husk extract led to a decrease in the accumulation of Tc-99m-radiopharmaceutical in certain organs of rats. The results obtained on the basis of the experimental model suggest that it is possible to use black walnut husk extract as a radioprotective agent in cases of planned exposure to ionizing radiation in diagnosticsnuclear medicine.",
publisher = "Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča", Društvo za zaštitu od zračenja Srbije i Crne Gore",
journal = "32. simpozijum DZZSCG : zbornik radova",
title = "Eksperimentalni model za procenu mogućeg radioprotektivnog efekta biljnog ekstrakta, Experimental Model for Assessing the Possible Radioprotective Effect of Plant Extract",
pages = "321-327",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12184"
}
Rajković, K. M., Đurašević, M., Obradović, Z., Milanović, Z., Vukadinović, A., Perić, M., Stanković, D.,& Janković, D.. (2023). Eksperimentalni model za procenu mogućeg radioprotektivnog efekta biljnog ekstrakta. in 32. simpozijum DZZSCG : zbornik radova
Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča"., 321-327.
https://hdl.handle.net/21.15107/rcub_vinar_12184
Rajković KM, Đurašević M, Obradović Z, Milanović Z, Vukadinović A, Perić M, Stanković D, Janković D. Eksperimentalni model za procenu mogućeg radioprotektivnog efekta biljnog ekstrakta. in 32. simpozijum DZZSCG : zbornik radova. 2023;:321-327.
https://hdl.handle.net/21.15107/rcub_vinar_12184 .
Rajković, Katarina M., Đurašević, Mirjana, Obradović, Zorica, Milanović, Zorana, Vukadinović, Aleksandar, Perić, Marko, Stanković, Dragana, Janković, Drina, "Eksperimentalni model za procenu mogućeg radioprotektivnog efekta biljnog ekstrakta" in 32. simpozijum DZZSCG : zbornik radova (2023):321-327,
https://hdl.handle.net/21.15107/rcub_vinar_12184 .

Validacija ITLC metode za određivanje sadržaja radiohemijske nečistoće C u 99mTc-MIBI injekciji

Janković, Drina; Mirković, Marija D.; Radović, Magdalena; Milanović, Zorana; Perić, Marko; Stanković, Dragana; Vukadinović, Aleksandar; Vranješ-Đurić, Sanja

(Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča", 2023)

TY  - CONF
AU  - Janković, Drina
AU  - Mirković, Marija D.
AU  - Radović, Magdalena
AU  - Milanović, Zorana
AU  - Perić, Marko
AU  - Stanković, Dragana
AU  - Vukadinović, Aleksandar
AU  - Vranješ-Đurić, Sanja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12185
AB  - Prisustvo radiohemijske nečistoće C u 99mTc-MIBI injekciji utiče na kvalitet skena, jer se radiofarmaceutik nedovoljno nakuplja u organu od interesa, dok je aktivnost okolnih organa i tkiva velika. Zbog toga je i doza zračenja koju prime okolni organi i tkiva iznad propisanih granica. Da bi se obezbedilo da je planirano izlaganje zračenju pacijenata svedeno na minimum, farmakopeja zahteva ispitivanje sadržaja radiohemijske nečistoće C u 99mTc-MIBI injekciji neposredno pre primene radiofarmaceutika u pacijenta. Za ova ispitivanja se koriste metode hromatografije. U radu je predstavljena brza i osetljiva ITLC metoda namenjena za rutinsko ispitivanje sadržaja radiohemijske nečistoće C u 99mTc-MIBI injekciji. ITLC metoda je validirana, a ispitivani su pogodnost sistema, tačnost, preciznost, ponovljivost, specifičnost, limit detekcije, limit kvantifikacije, linearnost, robustnost i osetljivost metode. Dobre "recovery" vrednosti i niska relativna standardna devijacija potvrđuju da je predložena ITLC metoda pogodna za rutinsko određivanje nečistoće C u 99mTc-MIBI u injekciji.
AB  - The European Pharmacopoeia mandates that all radiopharmaceuticals used in nuclear medicine for diagnostic and therapeutic purposes must be of the correct radiochemical and radionuclidic purity and have the correct radioactivity present at the stated time of injection to ensure that the intended radiation exposure of patients is kept to a minimum. These factors have an effect on the overall radiation dose to the patient, as impurities of the radionuclide and/or its chemical composition may affect the biodistribution of the injected radiopharmaceutical and consequently the radiation dose to any one particular organ or the whole body dose. The presence of radiochemical impurity C in 99mTc-MIBI injection affects the quality of the image, because 99mTc-MIBI accumulates insufficiently in the organ of interest, while the activity of the surrounding organs and tissues is high. Therefore, the radiation dose received by the surrounding organs and tissues is above the permitted level. In order to avoid unnecessary irradiation of surrounding organs and tissues, the pharmacopoeia requires examination of the content of radiochemical impurity C immediately before administering of 99mTc-MIBI to the patient. Chromatographic methods are used for these tests. The paper presents a fast and sensitive ITLC method intended for routine examination of the content of radiochemical impurity C in 99mTc-MIBI injection. The ITLC method was validated, and the suitability of the system, accuracy, precision, repeatability, specificity, limit of detection, limit of quantification, linearity, robustness and sensitivity of the method were examined. Good "recovery" values and low relative standard deviation confirm that the proposed ITLC method is suitable for routine determination of impurity C in 99mTc-MIBI in injection.
PB  - Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča"
PB  - Društvo za zaštitu od zračenja Srbije i Crne Gore
C3  - 32. simpozijum DZZSCG : zbornik radova
T1  - Validacija ITLC metode za određivanje sadržaja radiohemijske nečistoće C u 99mTc-MIBI injekciji
T1  - Validation of an ITLC Method for the Determination of Radiochemical Impurities C in 99mTc-MIBI Injection
SP  - 342
EP  - 349
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12185
ER  - 
@conference{
author = "Janković, Drina and Mirković, Marija D. and Radović, Magdalena and Milanović, Zorana and Perić, Marko and Stanković, Dragana and Vukadinović, Aleksandar and Vranješ-Đurić, Sanja",
year = "2023",
abstract = "Prisustvo radiohemijske nečistoće C u 99mTc-MIBI injekciji utiče na kvalitet skena, jer se radiofarmaceutik nedovoljno nakuplja u organu od interesa, dok je aktivnost okolnih organa i tkiva velika. Zbog toga je i doza zračenja koju prime okolni organi i tkiva iznad propisanih granica. Da bi se obezbedilo da je planirano izlaganje zračenju pacijenata svedeno na minimum, farmakopeja zahteva ispitivanje sadržaja radiohemijske nečistoće C u 99mTc-MIBI injekciji neposredno pre primene radiofarmaceutika u pacijenta. Za ova ispitivanja se koriste metode hromatografije. U radu je predstavljena brza i osetljiva ITLC metoda namenjena za rutinsko ispitivanje sadržaja radiohemijske nečistoće C u 99mTc-MIBI injekciji. ITLC metoda je validirana, a ispitivani su pogodnost sistema, tačnost, preciznost, ponovljivost, specifičnost, limit detekcije, limit kvantifikacije, linearnost, robustnost i osetljivost metode. Dobre "recovery" vrednosti i niska relativna standardna devijacija potvrđuju da je predložena ITLC metoda pogodna za rutinsko određivanje nečistoće C u 99mTc-MIBI u injekciji., The European Pharmacopoeia mandates that all radiopharmaceuticals used in nuclear medicine for diagnostic and therapeutic purposes must be of the correct radiochemical and radionuclidic purity and have the correct radioactivity present at the stated time of injection to ensure that the intended radiation exposure of patients is kept to a minimum. These factors have an effect on the overall radiation dose to the patient, as impurities of the radionuclide and/or its chemical composition may affect the biodistribution of the injected radiopharmaceutical and consequently the radiation dose to any one particular organ or the whole body dose. The presence of radiochemical impurity C in 99mTc-MIBI injection affects the quality of the image, because 99mTc-MIBI accumulates insufficiently in the organ of interest, while the activity of the surrounding organs and tissues is high. Therefore, the radiation dose received by the surrounding organs and tissues is above the permitted level. In order to avoid unnecessary irradiation of surrounding organs and tissues, the pharmacopoeia requires examination of the content of radiochemical impurity C immediately before administering of 99mTc-MIBI to the patient. Chromatographic methods are used for these tests. The paper presents a fast and sensitive ITLC method intended for routine examination of the content of radiochemical impurity C in 99mTc-MIBI injection. The ITLC method was validated, and the suitability of the system, accuracy, precision, repeatability, specificity, limit of detection, limit of quantification, linearity, robustness and sensitivity of the method were examined. Good "recovery" values and low relative standard deviation confirm that the proposed ITLC method is suitable for routine determination of impurity C in 99mTc-MIBI in injection.",
publisher = "Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča", Društvo za zaštitu od zračenja Srbije i Crne Gore",
journal = "32. simpozijum DZZSCG : zbornik radova",
title = "Validacija ITLC metode za određivanje sadržaja radiohemijske nečistoće C u 99mTc-MIBI injekciji, Validation of an ITLC Method for the Determination of Radiochemical Impurities C in 99mTc-MIBI Injection",
pages = "342-349",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12185"
}
Janković, D., Mirković, M. D., Radović, M., Milanović, Z., Perić, M., Stanković, D., Vukadinović, A.,& Vranješ-Đurić, S.. (2023). Validacija ITLC metode za određivanje sadržaja radiohemijske nečistoće C u 99mTc-MIBI injekciji. in 32. simpozijum DZZSCG : zbornik radova
Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča"., 342-349.
https://hdl.handle.net/21.15107/rcub_vinar_12185
Janković D, Mirković MD, Radović M, Milanović Z, Perić M, Stanković D, Vukadinović A, Vranješ-Đurić S. Validacija ITLC metode za određivanje sadržaja radiohemijske nečistoće C u 99mTc-MIBI injekciji. in 32. simpozijum DZZSCG : zbornik radova. 2023;:342-349.
https://hdl.handle.net/21.15107/rcub_vinar_12185 .
Janković, Drina, Mirković, Marija D., Radović, Magdalena, Milanović, Zorana, Perić, Marko, Stanković, Dragana, Vukadinović, Aleksandar, Vranješ-Đurić, Sanja, "Validacija ITLC metode za određivanje sadržaja radiohemijske nečistoće C u 99mTc-MIBI injekciji" in 32. simpozijum DZZSCG : zbornik radova (2023):342-349,
https://hdl.handle.net/21.15107/rcub_vinar_12185 .

Metoda ispitivanja fiziološke raspodele 99mTc-DPD

Milanović, Zorana; Mirković, Marija D.; Radović, Magdalena; Perić, Marko; Stanković, Dragana; Vukadinović, Aleksandar; Vranješ-Đurić, Sanja; Janković, Drina

(Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča", 2023)

TY  - CONF
AU  - Milanović, Zorana
AU  - Mirković, Marija D.
AU  - Radović, Magdalena
AU  - Perić, Marko
AU  - Stanković, Dragana
AU  - Vukadinović, Aleksandar
AU  - Vranješ-Đurić, Sanja
AU  - Janković, Drina
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12186
AB  - Radiofarmaceutici koji se koriste za ispitivanje skeletnog sistema od neprocenjivog su značaja u nuklearnoj medicini, kako za dijagnostiku primarnih tumora koštanog tkiva, tako i metastaza. Pre primene na pacijentima, ovi radiofarmaceutici podležu različitim fizičko-hemijskim i biološkim ispitivanjima. Ispitivanje fiziološke raspodele leka in vivo je od presudnog značaja jer od akumulacije leka u odgovarajućoj meri u ciljnom organu (skeletu) zavisi kvalitet dobijenog scintigrama, kao i doza zračenja koje će primiti pojedinačni organi i tkiva. U evropskoj farmakopeji (Ph.Eur.) date su metode kontrole kvaliteta kao i parametri kvaliteta sa granicama prihvatljivosti (specifikacijske granice) za 99mTc-metilendifosfonat (99mTc-MDP). Cilj ovog rada je prikaz metode ispitivanja fiziološke raspodele 99mTc-DPD koja je razvijena u Laboratoriji za radioizotope po smernicama evropske farmakopeje za 99mTc-MDP, uz manje modifikacije. Rezultati biodistribucije na Wistar pacovima su pokazali da je 99mTc-DPD proizveden u Laboratoriji za radioizotope zadovoljio sve postavljene kriterijume, kako odmah nakon proizvodnje, tako i nakon šest i dvanaest meseci od proizvodnje.
AB  - Radiopharmaceuticals used to examine the skeletal system are of invaluable importance in nuclear medicine, both for the diagnosis of primary bone tissue tumors and metastases. Before administration to patients, these radiopharmaceuticals undergo various physico-chemical and biological tests. Investigation of the physiological distribution of the drug in vivo is of crucial importance because the quality of the obtained scintigram depends on the accumulation of the drug in the target organ (skeleton), as well as the radiation dose received by individual organs and tissues. The European pharmacopoeia (Ph.Eur.) provides quality control methods and quality parameters with acceptance limits (specification limits) for 99mTc-methylenediphosphonate (99mTc-MDP). The method of testing the physiological distribution of 99mTc-DPD in the Laboratory for Radioisotopes, which is presented in this paper, is done according to the guidelines of the European Pharmacopoeia for 99mTc-MDP, with minor modifications. Results of biodistribution on Wistar rats showed that 99mTc-DPD produced in the Laboratory for Radioisotopes met all the set criteria, both immediately after the production and after six and twelve months from production.
PB  - Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča"
PB  - Društvo za zaštitu od zračenja Srbije i Crne Gore
C3  - 32. simpozijum DZZSCG : zbornik radova
T1  - Metoda ispitivanja fiziološke raspodele 99mTc-DPD
T1  - Method for Investigation of Physiological Distribution of 99mTc DPD
SP  - 350
EP  - 355
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12186
ER  - 
@conference{
author = "Milanović, Zorana and Mirković, Marija D. and Radović, Magdalena and Perić, Marko and Stanković, Dragana and Vukadinović, Aleksandar and Vranješ-Đurić, Sanja and Janković, Drina",
year = "2023",
abstract = "Radiofarmaceutici koji se koriste za ispitivanje skeletnog sistema od neprocenjivog su značaja u nuklearnoj medicini, kako za dijagnostiku primarnih tumora koštanog tkiva, tako i metastaza. Pre primene na pacijentima, ovi radiofarmaceutici podležu različitim fizičko-hemijskim i biološkim ispitivanjima. Ispitivanje fiziološke raspodele leka in vivo je od presudnog značaja jer od akumulacije leka u odgovarajućoj meri u ciljnom organu (skeletu) zavisi kvalitet dobijenog scintigrama, kao i doza zračenja koje će primiti pojedinačni organi i tkiva. U evropskoj farmakopeji (Ph.Eur.) date su metode kontrole kvaliteta kao i parametri kvaliteta sa granicama prihvatljivosti (specifikacijske granice) za 99mTc-metilendifosfonat (99mTc-MDP). Cilj ovog rada je prikaz metode ispitivanja fiziološke raspodele 99mTc-DPD koja je razvijena u Laboratoriji za radioizotope po smernicama evropske farmakopeje za 99mTc-MDP, uz manje modifikacije. Rezultati biodistribucije na Wistar pacovima su pokazali da je 99mTc-DPD proizveden u Laboratoriji za radioizotope zadovoljio sve postavljene kriterijume, kako odmah nakon proizvodnje, tako i nakon šest i dvanaest meseci od proizvodnje., Radiopharmaceuticals used to examine the skeletal system are of invaluable importance in nuclear medicine, both for the diagnosis of primary bone tissue tumors and metastases. Before administration to patients, these radiopharmaceuticals undergo various physico-chemical and biological tests. Investigation of the physiological distribution of the drug in vivo is of crucial importance because the quality of the obtained scintigram depends on the accumulation of the drug in the target organ (skeleton), as well as the radiation dose received by individual organs and tissues. The European pharmacopoeia (Ph.Eur.) provides quality control methods and quality parameters with acceptance limits (specification limits) for 99mTc-methylenediphosphonate (99mTc-MDP). The method of testing the physiological distribution of 99mTc-DPD in the Laboratory for Radioisotopes, which is presented in this paper, is done according to the guidelines of the European Pharmacopoeia for 99mTc-MDP, with minor modifications. Results of biodistribution on Wistar rats showed that 99mTc-DPD produced in the Laboratory for Radioisotopes met all the set criteria, both immediately after the production and after six and twelve months from production.",
publisher = "Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča", Društvo za zaštitu od zračenja Srbije i Crne Gore",
journal = "32. simpozijum DZZSCG : zbornik radova",
title = "Metoda ispitivanja fiziološke raspodele 99mTc-DPD, Method for Investigation of Physiological Distribution of 99mTc DPD",
pages = "350-355",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12186"
}
Milanović, Z., Mirković, M. D., Radović, M., Perić, M., Stanković, D., Vukadinović, A., Vranješ-Đurić, S.,& Janković, D.. (2023). Metoda ispitivanja fiziološke raspodele 99mTc-DPD. in 32. simpozijum DZZSCG : zbornik radova
Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča"., 350-355.
https://hdl.handle.net/21.15107/rcub_vinar_12186
Milanović Z, Mirković MD, Radović M, Perić M, Stanković D, Vukadinović A, Vranješ-Đurić S, Janković D. Metoda ispitivanja fiziološke raspodele 99mTc-DPD. in 32. simpozijum DZZSCG : zbornik radova. 2023;:350-355.
https://hdl.handle.net/21.15107/rcub_vinar_12186 .
Milanović, Zorana, Mirković, Marija D., Radović, Magdalena, Perić, Marko, Stanković, Dragana, Vukadinović, Aleksandar, Vranješ-Đurić, Sanja, Janković, Drina, "Metoda ispitivanja fiziološke raspodele 99mTc-DPD" in 32. simpozijum DZZSCG : zbornik radova (2023):350-355,
https://hdl.handle.net/21.15107/rcub_vinar_12186 .

Automatizacija procesa proizvodnje radiofarmaceutika u cilju smanjenja doze zračenja operatera

Vukadinović, Aleksandar; Ravlić, Miroslav; Matović, Milovan; Janković, Drina; Mirković, Marija D.; Radović, Magdalena; Milanović, Zorana; Perić, Marko; Stanković, Dragana; Jevremović, Milutin; Vranješ-Đurić, Sanja

(Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča", 2023)

TY  - CONF
AU  - Vukadinović, Aleksandar
AU  - Ravlić, Miroslav
AU  - Matović, Milovan
AU  - Janković, Drina
AU  - Mirković, Marija D.
AU  - Radović, Magdalena
AU  - Milanović, Zorana
AU  - Perić, Marko
AU  - Stanković, Dragana
AU  - Jevremović, Milutin
AU  - Vranješ-Đurić, Sanja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12187
AB  - Laboratorija za radioizotope Instituta Vinča je jedinstven centar u regionu koji poseduje Rešenja Ministarstva zdravlja Republike Srbije i Direktorata za radijacionu i nuklearnu sigurnost i bezbednost Srbije za proizvodnju radiofarmaceutika. Radiofarmaceutik koji se proizvodi više od 40 godina u Laboratoriji za radioizotope, a primenjuje u terapiji tumora štitaste žlezde, kao i hipertireozi su kapsule natrijum-jodida (I-131). Radijacioni efekti I-131 na ćelije štitaste žlezde potiču od beta-zračenja koje emituje I-131 tokom radioaktivnog raspada u samoj štitastoj žlezdi jer se I-131 nakon oralne primene nakuplja prvenstveno u štitastoj žlezdi u kojoj ima dugu retenciju. Za efikasnu terapiju, ali u isto vreme u cilju smanjenja izloženosti nepotrebnim dozama, za svakog pacijenta određuje se odgovarajuća doza I-131 koju je neophodno da primi, odnosno primenjuje se tzv. personalizovana terapija. U radu je opisan postupak proizvodnje kapsula I-131 u Laboratoriji za radioizotope putem manuelnog punjenja kapsula rastvorom I-131, kao i osnovni zahtevi koje je neophodno da sistem za automatsku proizvodnju radiofarmaceutika ispuni, a to su pre svih, odgovarajuća zaštita operatera od zračenja, jednostavna upotreba i kompjuterska kontrola.
AB  - The Laboratory for radioisotopes of the Vinča Institute is a unique center in the region with licences of the Ministry of Health of the Republic of Serbia and the Directorate for Radiation and Nuclear Safety and Security of Serbia for the production of radiopharmaceuticals. Sodium iodide (I-131) capsules are radiopharmaceuticals that have been produced for more than 40 years in the Laboratory for Radioisotopes, and are used in the treatment of tumors of thyroid gland, as well as in hyperthyroidism. The radiation effects of I-131 on thyroid cells originate from beta-radiation emitted by I-131 during radioactive decay in the thyroid gland itself because I-131 after oral administration accumulates primarily in the thyroid gland where it has a long retention. For the effective therapy, but at the same time in order to reduce exposure to unnecessary high doses, the appropriate dose of I-131 is determined for each patient, in the so-called personalized therapy. The paper describes the current procedure for the production of I-131 capsules in the Laboratory for Radioisotopes by manually filling the capsules with I-131 solution and the basic requirements for design the system for automating the production of radiopharmaceuticals, which above all must provide adequate protection for the operator from radiation, must be user friendly and computer-controlled.
PB  - Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča"
PB  - Društvo za zaštitu od zračenja Srbije i Crne Gore
C3  - 32. simpozijum DZZSCG : zbornik radova
T1  - Automatizacija procesa proizvodnje radiofarmaceutika u cilju smanjenja doze zračenja operatera
T1  - Automation of the Production of Radiopharmaceutical with the Aim to Reduce the Operator's Radiation Dose
SP  - 356
EP  - 360
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12187
ER  - 
@conference{
author = "Vukadinović, Aleksandar and Ravlić, Miroslav and Matović, Milovan and Janković, Drina and Mirković, Marija D. and Radović, Magdalena and Milanović, Zorana and Perić, Marko and Stanković, Dragana and Jevremović, Milutin and Vranješ-Đurić, Sanja",
year = "2023",
abstract = "Laboratorija za radioizotope Instituta Vinča je jedinstven centar u regionu koji poseduje Rešenja Ministarstva zdravlja Republike Srbije i Direktorata za radijacionu i nuklearnu sigurnost i bezbednost Srbije za proizvodnju radiofarmaceutika. Radiofarmaceutik koji se proizvodi više od 40 godina u Laboratoriji za radioizotope, a primenjuje u terapiji tumora štitaste žlezde, kao i hipertireozi su kapsule natrijum-jodida (I-131). Radijacioni efekti I-131 na ćelije štitaste žlezde potiču od beta-zračenja koje emituje I-131 tokom radioaktivnog raspada u samoj štitastoj žlezdi jer se I-131 nakon oralne primene nakuplja prvenstveno u štitastoj žlezdi u kojoj ima dugu retenciju. Za efikasnu terapiju, ali u isto vreme u cilju smanjenja izloženosti nepotrebnim dozama, za svakog pacijenta određuje se odgovarajuća doza I-131 koju je neophodno da primi, odnosno primenjuje se tzv. personalizovana terapija. U radu je opisan postupak proizvodnje kapsula I-131 u Laboratoriji za radioizotope putem manuelnog punjenja kapsula rastvorom I-131, kao i osnovni zahtevi koje je neophodno da sistem za automatsku proizvodnju radiofarmaceutika ispuni, a to su pre svih, odgovarajuća zaštita operatera od zračenja, jednostavna upotreba i kompjuterska kontrola., The Laboratory for radioisotopes of the Vinča Institute is a unique center in the region with licences of the Ministry of Health of the Republic of Serbia and the Directorate for Radiation and Nuclear Safety and Security of Serbia for the production of radiopharmaceuticals. Sodium iodide (I-131) capsules are radiopharmaceuticals that have been produced for more than 40 years in the Laboratory for Radioisotopes, and are used in the treatment of tumors of thyroid gland, as well as in hyperthyroidism. The radiation effects of I-131 on thyroid cells originate from beta-radiation emitted by I-131 during radioactive decay in the thyroid gland itself because I-131 after oral administration accumulates primarily in the thyroid gland where it has a long retention. For the effective therapy, but at the same time in order to reduce exposure to unnecessary high doses, the appropriate dose of I-131 is determined for each patient, in the so-called personalized therapy. The paper describes the current procedure for the production of I-131 capsules in the Laboratory for Radioisotopes by manually filling the capsules with I-131 solution and the basic requirements for design the system for automating the production of radiopharmaceuticals, which above all must provide adequate protection for the operator from radiation, must be user friendly and computer-controlled.",
publisher = "Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča", Društvo za zaštitu od zračenja Srbije i Crne Gore",
journal = "32. simpozijum DZZSCG : zbornik radova",
title = "Automatizacija procesa proizvodnje radiofarmaceutika u cilju smanjenja doze zračenja operatera, Automation of the Production of Radiopharmaceutical with the Aim to Reduce the Operator's Radiation Dose",
pages = "356-360",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12187"
}
Vukadinović, A., Ravlić, M., Matović, M., Janković, D., Mirković, M. D., Radović, M., Milanović, Z., Perić, M., Stanković, D., Jevremović, M.,& Vranješ-Đurić, S.. (2023). Automatizacija procesa proizvodnje radiofarmaceutika u cilju smanjenja doze zračenja operatera. in 32. simpozijum DZZSCG : zbornik radova
Beograd : Univerzitet u Beogradu, Institut za nuklearne nauke "Vinča"., 356-360.
https://hdl.handle.net/21.15107/rcub_vinar_12187
Vukadinović A, Ravlić M, Matović M, Janković D, Mirković MD, Radović M, Milanović Z, Perić M, Stanković D, Jevremović M, Vranješ-Đurić S. Automatizacija procesa proizvodnje radiofarmaceutika u cilju smanjenja doze zračenja operatera. in 32. simpozijum DZZSCG : zbornik radova. 2023;:356-360.
https://hdl.handle.net/21.15107/rcub_vinar_12187 .
Vukadinović, Aleksandar, Ravlić, Miroslav, Matović, Milovan, Janković, Drina, Mirković, Marija D., Radović, Magdalena, Milanović, Zorana, Perić, Marko, Stanković, Dragana, Jevremović, Milutin, Vranješ-Đurić, Sanja, "Automatizacija procesa proizvodnje radiofarmaceutika u cilju smanjenja doze zračenja operatera" in 32. simpozijum DZZSCG : zbornik radova (2023):356-360,
https://hdl.handle.net/21.15107/rcub_vinar_12187 .

DFT analysis of hyperfine couplings in d and f metal complexes with tetrahydro borate ligands

Perić, Marko; Milanović, Zorana; Radović, Magdalena; Mirković, Marija D.

(2023)

TY  - JOUR
AU  - Perić, Marko
AU  - Milanović, Zorana
AU  - Radović, Magdalena
AU  - Mirković, Marija D.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11432
AB  - Metal complexes with BH4- ligands show extravagant structural and dynamic properties, and possess many important and applicable qualities (potent reducing agents and catalysts, materials for hydrogen storage). Electron paramagnetic resonance (EPR) is very important for the characterization of complex compounds, the determination of their electronic configuration and geometry. Also, Density Functional Theory (DFT) can predict EPR parameters and explain them more profoundly. Hence, in this paper hyperfine coupling constants of d and f metal complexes with BH4- ligands have been determined by DFT calculations, and analyzed in detail. Of particular importance is the analysis and prediction of hyperfine coupling constants for lanthanide complexes with BH4- ligands, given that there is not much data in the literature. Calculations predicted that proton hyperfine coupling constants are very small in the case of complexes of f elements, and are present only due to weak polarization. The increase of covalence and the number of unpaired electrons does not significantly affect the change of constants of BH4- ligands, but only of protons that are bound by σ bonds.
T2  - Polyhedron
T1  - DFT analysis of hyperfine couplings in d and f metal complexes with tetrahydro borate ligands
VL  - 244
SP  - 116616
DO  - 10.1016/j.poly.2023.116616
ER  - 
@article{
author = "Perić, Marko and Milanović, Zorana and Radović, Magdalena and Mirković, Marija D.",
year = "2023",
abstract = "Metal complexes with BH4- ligands show extravagant structural and dynamic properties, and possess many important and applicable qualities (potent reducing agents and catalysts, materials for hydrogen storage). Electron paramagnetic resonance (EPR) is very important for the characterization of complex compounds, the determination of their electronic configuration and geometry. Also, Density Functional Theory (DFT) can predict EPR parameters and explain them more profoundly. Hence, in this paper hyperfine coupling constants of d and f metal complexes with BH4- ligands have been determined by DFT calculations, and analyzed in detail. Of particular importance is the analysis and prediction of hyperfine coupling constants for lanthanide complexes with BH4- ligands, given that there is not much data in the literature. Calculations predicted that proton hyperfine coupling constants are very small in the case of complexes of f elements, and are present only due to weak polarization. The increase of covalence and the number of unpaired electrons does not significantly affect the change of constants of BH4- ligands, but only of protons that are bound by σ bonds.",
journal = "Polyhedron",
title = "DFT analysis of hyperfine couplings in d and f metal complexes with tetrahydro borate ligands",
volume = "244",
pages = "116616",
doi = "10.1016/j.poly.2023.116616"
}
Perić, M., Milanović, Z., Radović, M.,& Mirković, M. D.. (2023). DFT analysis of hyperfine couplings in d and f metal complexes with tetrahydro borate ligands. in Polyhedron, 244, 116616.
https://doi.org/10.1016/j.poly.2023.116616
Perić M, Milanović Z, Radović M, Mirković MD. DFT analysis of hyperfine couplings in d and f metal complexes with tetrahydro borate ligands. in Polyhedron. 2023;244:116616.
doi:10.1016/j.poly.2023.116616 .
Perić, Marko, Milanović, Zorana, Radović, Magdalena, Mirković, Marija D., "DFT analysis of hyperfine couplings in d and f metal complexes with tetrahydro borate ligands" in Polyhedron, 244 (2023):116616,
https://doi.org/10.1016/j.poly.2023.116616 . .

DFT Analysis of Hyperfine Couplings in d and f metal complexes with Tetrahydro Borate Ligands

Perić, Marko; Milanović, Zorana; Radović, Magdalena; Mirković, M.; Vukadinović, Aleksandar; Stanković, D.; Janković, Drina; Vranješ-Đurić, Sanja

(Belgrade : Serbian Ceramic Society, 2023)

TY  - CONF
AU  - Perić, Marko
AU  - Milanović, Zorana
AU  - Radović, Magdalena
AU  - Mirković, M.
AU  - Vukadinović, Aleksandar
AU  - Stanković, D.
AU  - Janković, Drina
AU  - Vranješ-Đurić, Sanja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11632
AB  - Metal complexes with BH4 - ligands show extravagant structural and dynamic properties, and possess many important and applicable qualities (potent reducing agents and catalysts, materials for hydrogen storage). Electron paramagnetic resonance (EPR) is very important for the characterization of complex compounds, determination of their electronic configuration and geometry. Also, Density Functional Theory (DFT) can predict EPR parameters and explain them more profoundly. Of particular importance is the analysis and prediction of hyperfine coupling constants for lanthanide complexes with BH4 - ligands, given that there is not much data in the literature. Within this paper hyperfine coupling constants of d and f metal complexes with BH4 - ligands have been determined by DFT calculations, and analyzed in detail. Calculations predicted that proton hyperfine coupling constants are very small in the case of complexes of f elements, and are present only due to weak polarization. The increase of covalence and the number of unpaired electrons does not significantly affect the change of constants of BH4 - ligands, but only of protons that are bound by σ bonds.
PB  - Belgrade : Serbian Ceramic Society
C3  - Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade
T1  - DFT Analysis of Hyperfine Couplings in d and f metal complexes with Tetrahydro Borate Ligands
SP  - 35
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11632
ER  - 
@conference{
author = "Perić, Marko and Milanović, Zorana and Radović, Magdalena and Mirković, M. and Vukadinović, Aleksandar and Stanković, D. and Janković, Drina and Vranješ-Đurić, Sanja",
year = "2023",
abstract = "Metal complexes with BH4 - ligands show extravagant structural and dynamic properties, and possess many important and applicable qualities (potent reducing agents and catalysts, materials for hydrogen storage). Electron paramagnetic resonance (EPR) is very important for the characterization of complex compounds, determination of their electronic configuration and geometry. Also, Density Functional Theory (DFT) can predict EPR parameters and explain them more profoundly. Of particular importance is the analysis and prediction of hyperfine coupling constants for lanthanide complexes with BH4 - ligands, given that there is not much data in the literature. Within this paper hyperfine coupling constants of d and f metal complexes with BH4 - ligands have been determined by DFT calculations, and analyzed in detail. Calculations predicted that proton hyperfine coupling constants are very small in the case of complexes of f elements, and are present only due to weak polarization. The increase of covalence and the number of unpaired electrons does not significantly affect the change of constants of BH4 - ligands, but only of protons that are bound by σ bonds.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade",
title = "DFT Analysis of Hyperfine Couplings in d and f metal complexes with Tetrahydro Borate Ligands",
pages = "35-36",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11632"
}
Perić, M., Milanović, Z., Radović, M., Mirković, M., Vukadinović, A., Stanković, D., Janković, D.,& Vranješ-Đurić, S.. (2023). DFT Analysis of Hyperfine Couplings in d and f metal complexes with Tetrahydro Borate Ligands. in Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade
Belgrade : Serbian Ceramic Society., 35-36.
https://hdl.handle.net/21.15107/rcub_vinar_11632
Perić M, Milanović Z, Radović M, Mirković M, Vukadinović A, Stanković D, Janković D, Vranješ-Đurić S. DFT Analysis of Hyperfine Couplings in d and f metal complexes with Tetrahydro Borate Ligands. in Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade. 2023;:35-36.
https://hdl.handle.net/21.15107/rcub_vinar_11632 .
Perić, Marko, Milanović, Zorana, Radović, Magdalena, Mirković, M., Vukadinović, Aleksandar, Stanković, D., Janković, Drina, Vranješ-Đurić, Sanja, "DFT Analysis of Hyperfine Couplings in d and f metal complexes with Tetrahydro Borate Ligands" in Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade (2023):35-36,
https://hdl.handle.net/21.15107/rcub_vinar_11632 .

90Y-CA/SPIONs for dual magnetic hyperthermia-radionuclide nanobrachytherapy of solid tumours

Vukadinović, Aleksandar; Milanović, Zorana; Ognjanović, Miloš; Janković, Drina; Radović, Magdalena; Mirković, Marija D.; Karageorgou, Maria-Argyro; Bouziotis, Penelope; Erić, Slavica; Vranješ-Đurić, Sanja; Antić, Bratislav; Prijović, Željko

(2022)

TY  - JOUR
AU  - Vukadinović, Aleksandar
AU  - Milanović, Zorana
AU  - Ognjanović, Miloš
AU  - Janković, Drina
AU  - Radović, Magdalena
AU  - Mirković, Marija D.
AU  - Karageorgou, Maria-Argyro
AU  - Bouziotis, Penelope
AU  - Erić, Slavica
AU  - Vranješ-Đurić, Sanja
AU  - Antić, Bratislav
AU  - Prijović, Željko
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10368
AB  - Radiolabelled superparamagnetic iron oxide nanoparticles (SPIONs) are a promising nanomaterial for the development of dual radiation/hyperthermia cancer therapy. To that purpose, flower-shaped SPIONs with an exceptional heating capability were synthesised and coated with citrate, dextran or (3-aminopropyl)triethoxysilane. Both non-coated and coated SPIONs were nontoxic to CT-26 mouse colon cancer cells up to 1.0 mg ml−1 in vitro. In an oscillating magnetic field, citrate-coated SPIONs (CA/SPIONs) displayed the highest heating rate (SAR ∼ 253 W g−1) and the strongest hyperthermia effects against CT-26 cells. Labelling of the CA/SPIONs by the 90Y radionuclide, emitting β− radiation with an average/maximum energy of 0.94/2.23 MeV, and deep tissue penetration generated 90Y-CA/SPIONs intended for the therapy of solid tumours. However, intravenous injection of 90Y-CA/SPIONs in CT-26 xenograft-bearing mice resulted in low tumour accumulation. On the contrary, intratumoural injection resulted in long-term retention at the injection site. A single intratumoural injection of 0.25 mg CA/SPIONs followed by 30-min courses of magnetic hyperthermia for four consecutive days caused a moderate antitumour effect against CT-26 and 4T1 mouse tumour xenografts. Intratumoural application of 1.85 MBq/0.25 mg 90Y-CA/SPIONs, alone or combined with hyperthermia, caused a significant (P ≤ 0.01) antitumour effect without signs of systemic toxicity. The results confirm the suitability of 90Y-CA/SPIONs for monotherapy or dual magnetic hyperthermia-radionuclide nanobrachytherapy (NBT) of solid tumours.
T2  - Nanotechnology
T1  - 90Y-CA/SPIONs for dual magnetic hyperthermia-radionuclide nanobrachytherapy of solid tumours
VL  - 33
IS  - 40
SP  - 405102
DO  - 10.1088/1361-6528/ac7ac0
ER  - 
@article{
author = "Vukadinović, Aleksandar and Milanović, Zorana and Ognjanović, Miloš and Janković, Drina and Radović, Magdalena and Mirković, Marija D. and Karageorgou, Maria-Argyro and Bouziotis, Penelope and Erić, Slavica and Vranješ-Đurić, Sanja and Antić, Bratislav and Prijović, Željko",
year = "2022",
abstract = "Radiolabelled superparamagnetic iron oxide nanoparticles (SPIONs) are a promising nanomaterial for the development of dual radiation/hyperthermia cancer therapy. To that purpose, flower-shaped SPIONs with an exceptional heating capability were synthesised and coated with citrate, dextran or (3-aminopropyl)triethoxysilane. Both non-coated and coated SPIONs were nontoxic to CT-26 mouse colon cancer cells up to 1.0 mg ml−1 in vitro. In an oscillating magnetic field, citrate-coated SPIONs (CA/SPIONs) displayed the highest heating rate (SAR ∼ 253 W g−1) and the strongest hyperthermia effects against CT-26 cells. Labelling of the CA/SPIONs by the 90Y radionuclide, emitting β− radiation with an average/maximum energy of 0.94/2.23 MeV, and deep tissue penetration generated 90Y-CA/SPIONs intended for the therapy of solid tumours. However, intravenous injection of 90Y-CA/SPIONs in CT-26 xenograft-bearing mice resulted in low tumour accumulation. On the contrary, intratumoural injection resulted in long-term retention at the injection site. A single intratumoural injection of 0.25 mg CA/SPIONs followed by 30-min courses of magnetic hyperthermia for four consecutive days caused a moderate antitumour effect against CT-26 and 4T1 mouse tumour xenografts. Intratumoural application of 1.85 MBq/0.25 mg 90Y-CA/SPIONs, alone or combined with hyperthermia, caused a significant (P ≤ 0.01) antitumour effect without signs of systemic toxicity. The results confirm the suitability of 90Y-CA/SPIONs for monotherapy or dual magnetic hyperthermia-radionuclide nanobrachytherapy (NBT) of solid tumours.",
journal = "Nanotechnology",
title = "90Y-CA/SPIONs for dual magnetic hyperthermia-radionuclide nanobrachytherapy of solid tumours",
volume = "33",
number = "40",
pages = "405102",
doi = "10.1088/1361-6528/ac7ac0"
}
Vukadinović, A., Milanović, Z., Ognjanović, M., Janković, D., Radović, M., Mirković, M. D., Karageorgou, M., Bouziotis, P., Erić, S., Vranješ-Đurić, S., Antić, B.,& Prijović, Ž.. (2022). 90Y-CA/SPIONs for dual magnetic hyperthermia-radionuclide nanobrachytherapy of solid tumours. in Nanotechnology, 33(40), 405102.
https://doi.org/10.1088/1361-6528/ac7ac0
Vukadinović A, Milanović Z, Ognjanović M, Janković D, Radović M, Mirković MD, Karageorgou M, Bouziotis P, Erić S, Vranješ-Đurić S, Antić B, Prijović Ž. 90Y-CA/SPIONs for dual magnetic hyperthermia-radionuclide nanobrachytherapy of solid tumours. in Nanotechnology. 2022;33(40):405102.
doi:10.1088/1361-6528/ac7ac0 .
Vukadinović, Aleksandar, Milanović, Zorana, Ognjanović, Miloš, Janković, Drina, Radović, Magdalena, Mirković, Marija D., Karageorgou, Maria-Argyro, Bouziotis, Penelope, Erić, Slavica, Vranješ-Đurić, Sanja, Antić, Bratislav, Prijović, Željko, "90Y-CA/SPIONs for dual magnetic hyperthermia-radionuclide nanobrachytherapy of solid tumours" in Nanotechnology, 33, no. 40 (2022):405102,
https://doi.org/10.1088/1361-6528/ac7ac0 . .
8
1
5

Design and preparation of proline, tryptophan and poly-l-lysine functionalized magnetic nanoparticles and their radiolabeling with 131I and 177Lu for potential theranostic use

Mirković, Marija D.; Milanović, Zorana; Perić, Marko R.; Vranješ-Đurić, Sanja; Ognjanović, Miloš; Antić, Bratislav; Kuraica, Milorad; Krstić, Ivan; Kubovcikova, Martina; Antal, Iryna; Sobotova, Radka; Zavisova, Vlasta; Jurikova, Alena; Fabian, Martin; Koneracka, Martina

(2022)

TY  - JOUR
AU  - Mirković, Marija D.
AU  - Milanović, Zorana
AU  - Perić, Marko R.
AU  - Vranješ-Đurić, Sanja
AU  - Ognjanović, Miloš
AU  - Antić, Bratislav
AU  - Kuraica, Milorad
AU  - Krstić, Ivan
AU  - Kubovcikova, Martina
AU  - Antal, Iryna
AU  - Sobotova, Radka
AU  - Zavisova, Vlasta
AU  - Jurikova, Alena
AU  - Fabian, Martin
AU  - Koneracka, Martina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10696
AB  - Surface modification of magnetic nanoparticles with poly-L-lysine, proline, and tryptophan was used to design potential theranostic agents for the application in cancer diagnosis and radionuclide-hyperthermia therapy. Characterization of bare and functionalized magnetic nanoparticles was performed in detail. The transparency of the examined magnetic nanoparticles was measured in the non-alternating magnetic field for a complete and better understanding of hyperthermia. For the first time amino acid-functionalized magnetic nanoparticles were labeled with theranostic radionuclides 131I and 177Lu. The specific absorption rate (SAR) procured for poly-L-lysine functionalized magnetic nanoparticles (SAR values of 99.7 W/g at H0 = 15.9 kA/m and resonant frequency of 252 kHz) demonstrated their possible application in magnetic hyperthermia. Poly-L-lysine functionalized magnetic nanoparticles labeled with 177Lu showed the highest radiochemical purity (>99.00 %) and in vitro stability in saline and serum (>98.00 % up to 96 h). The in vivo analysis performed after their intravenous administration in healthy Wistar rats presented good in vivo stability for several days. Encouraging results as well as magnetic and radiochemical properties of 177Lu–PLL-MNPs (80 °C) justify their further testing toward the potential use as theranostic agents for diagnostic and combined radionuclide-hyperthermia therapeutic applications.
T2  - International Journal of Pharmaceutics
T1  - Design and preparation of proline, tryptophan and poly-l-lysine functionalized magnetic nanoparticles and their radiolabeling with 131I and 177Lu for potential theranostic use
VL  - 628
SP  - 122288
DO  - 10.1016/j.ijpharm.2022.122288
ER  - 
@article{
author = "Mirković, Marija D. and Milanović, Zorana and Perić, Marko R. and Vranješ-Đurić, Sanja and Ognjanović, Miloš and Antić, Bratislav and Kuraica, Milorad and Krstić, Ivan and Kubovcikova, Martina and Antal, Iryna and Sobotova, Radka and Zavisova, Vlasta and Jurikova, Alena and Fabian, Martin and Koneracka, Martina",
year = "2022",
abstract = "Surface modification of magnetic nanoparticles with poly-L-lysine, proline, and tryptophan was used to design potential theranostic agents for the application in cancer diagnosis and radionuclide-hyperthermia therapy. Characterization of bare and functionalized magnetic nanoparticles was performed in detail. The transparency of the examined magnetic nanoparticles was measured in the non-alternating magnetic field for a complete and better understanding of hyperthermia. For the first time amino acid-functionalized magnetic nanoparticles were labeled with theranostic radionuclides 131I and 177Lu. The specific absorption rate (SAR) procured for poly-L-lysine functionalized magnetic nanoparticles (SAR values of 99.7 W/g at H0 = 15.9 kA/m and resonant frequency of 252 kHz) demonstrated their possible application in magnetic hyperthermia. Poly-L-lysine functionalized magnetic nanoparticles labeled with 177Lu showed the highest radiochemical purity (>99.00 %) and in vitro stability in saline and serum (>98.00 % up to 96 h). The in vivo analysis performed after their intravenous administration in healthy Wistar rats presented good in vivo stability for several days. Encouraging results as well as magnetic and radiochemical properties of 177Lu–PLL-MNPs (80 °C) justify their further testing toward the potential use as theranostic agents for diagnostic and combined radionuclide-hyperthermia therapeutic applications.",
journal = "International Journal of Pharmaceutics",
title = "Design and preparation of proline, tryptophan and poly-l-lysine functionalized magnetic nanoparticles and their radiolabeling with 131I and 177Lu for potential theranostic use",
volume = "628",
pages = "122288",
doi = "10.1016/j.ijpharm.2022.122288"
}
Mirković, M. D., Milanović, Z., Perić, M. R., Vranješ-Đurić, S., Ognjanović, M., Antić, B., Kuraica, M., Krstić, I., Kubovcikova, M., Antal, I., Sobotova, R., Zavisova, V., Jurikova, A., Fabian, M.,& Koneracka, M.. (2022). Design and preparation of proline, tryptophan and poly-l-lysine functionalized magnetic nanoparticles and their radiolabeling with 131I and 177Lu for potential theranostic use. in International Journal of Pharmaceutics, 628, 122288.
https://doi.org/10.1016/j.ijpharm.2022.122288
Mirković MD, Milanović Z, Perić MR, Vranješ-Đurić S, Ognjanović M, Antić B, Kuraica M, Krstić I, Kubovcikova M, Antal I, Sobotova R, Zavisova V, Jurikova A, Fabian M, Koneracka M. Design and preparation of proline, tryptophan and poly-l-lysine functionalized magnetic nanoparticles and their radiolabeling with 131I and 177Lu for potential theranostic use. in International Journal of Pharmaceutics. 2022;628:122288.
doi:10.1016/j.ijpharm.2022.122288 .
Mirković, Marija D., Milanović, Zorana, Perić, Marko R., Vranješ-Đurić, Sanja, Ognjanović, Miloš, Antić, Bratislav, Kuraica, Milorad, Krstić, Ivan, Kubovcikova, Martina, Antal, Iryna, Sobotova, Radka, Zavisova, Vlasta, Jurikova, Alena, Fabian, Martin, Koneracka, Martina, "Design and preparation of proline, tryptophan and poly-l-lysine functionalized magnetic nanoparticles and their radiolabeling with 131I and 177Lu for potential theranostic use" in International Journal of Pharmaceutics, 628 (2022):122288,
https://doi.org/10.1016/j.ijpharm.2022.122288 . .
5
5

The effect of Juglans nigra L. green husk extracts on the biodistribution of radiopharmaceutical sodium pertechnetate in mice

Rajković, Katarina; Đurašević, Mirjana M.; Milanović, Zorana; Vranješ-Đurić, Sanja; Janković, Drina; Mirković, Marija; Obradović, Zorica

(RAD Centre, Niš, Serbia, 2022)

TY  - CONF
AU  - Rajković, Katarina
AU  - Đurašević, Mirjana M.
AU  - Milanović, Zorana
AU  - Vranješ-Đurić, Sanja
AU  - Janković, Drina
AU  - Mirković, Marija
AU  - Obradović, Zorica
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11109
AB  - Juglans nigra L. (Black walnut) green husk contains a variety of useful chemical compounds with numerous health benefits. However, the biological effects of these compounds are not fully known. The aim of this research was to evaluate the effect of the extracts from J. nigra green husks on the biodistribution of the sodium pertechenetate (Na99mTcO4). The extract was orally administered to the healthy Wistar rats (male, 1-month-old, weighing 89.4±3.4g) at single doses of 13.7 mg/kg/day by gavage for 10 days. On the eleventh day, 0.1 ml (approximately 148 kBq) of the Na99mTcO4 was injected into the tail vein. Rats were sacrificed at different time intervals and the radioactivity in the organs of interest was measured in a gamma counter with a NaI (Tl) detector. The percentage of radioactivity per organ (%ID/organ) was calculated. The organ uptake of the Na99mTcO4 in an additional control group of animals was also studied. The results obtained showed an alteration in the organ uptake of Na99mTcO4 in rats treated with extract. The radiopharmaceutical Na99mTcO4 is generally distributed throughout the vasculature and interstitial fluid and is concentrated in the stomach, intestinal tract, thyroid and salivary glands. After treatment of rats with the extract, there was a statistically significant decrease (p<0.05) in the uptake of Na99mTcO4 (%ID/organ) in the thyroid, heart, kidneys, liver and colon, and an increase in intestinal uptake compared to controls. These results are associated with properties of the chemical compounds present in the J. nigra extract. We assume that the compounds from the extract J. nigra could promote physiological modifications in these organs and alter the biodistribution of Na99mTcO4 in the treated animals. Although these research studies were performed in animals, the findings suggest that caution should be exercised while interpreting the results of Na99mTcO4 based nuclear medicine examinations in patients using J. nigra extract from green husk.
PB  - RAD Centre, Niš, Serbia
C3  - RAD 2022 : 10th Jubilee International Conference on Radiation in Various Fields of Research : book of abstracts; July 25-29; Herceg Novi, Montenegro
T1  - The effect of Juglans nigra L. green husk extracts on the biodistribution of radiopharmaceutical sodium pertechnetate in mice
SP  - 119
DO  - 10.21175/rad.sum.abstr.book.2022.29.1
ER  - 
@conference{
author = "Rajković, Katarina and Đurašević, Mirjana M. and Milanović, Zorana and Vranješ-Đurić, Sanja and Janković, Drina and Mirković, Marija and Obradović, Zorica",
year = "2022",
abstract = "Juglans nigra L. (Black walnut) green husk contains a variety of useful chemical compounds with numerous health benefits. However, the biological effects of these compounds are not fully known. The aim of this research was to evaluate the effect of the extracts from J. nigra green husks on the biodistribution of the sodium pertechenetate (Na99mTcO4). The extract was orally administered to the healthy Wistar rats (male, 1-month-old, weighing 89.4±3.4g) at single doses of 13.7 mg/kg/day by gavage for 10 days. On the eleventh day, 0.1 ml (approximately 148 kBq) of the Na99mTcO4 was injected into the tail vein. Rats were sacrificed at different time intervals and the radioactivity in the organs of interest was measured in a gamma counter with a NaI (Tl) detector. The percentage of radioactivity per organ (%ID/organ) was calculated. The organ uptake of the Na99mTcO4 in an additional control group of animals was also studied. The results obtained showed an alteration in the organ uptake of Na99mTcO4 in rats treated with extract. The radiopharmaceutical Na99mTcO4 is generally distributed throughout the vasculature and interstitial fluid and is concentrated in the stomach, intestinal tract, thyroid and salivary glands. After treatment of rats with the extract, there was a statistically significant decrease (p<0.05) in the uptake of Na99mTcO4 (%ID/organ) in the thyroid, heart, kidneys, liver and colon, and an increase in intestinal uptake compared to controls. These results are associated with properties of the chemical compounds present in the J. nigra extract. We assume that the compounds from the extract J. nigra could promote physiological modifications in these organs and alter the biodistribution of Na99mTcO4 in the treated animals. Although these research studies were performed in animals, the findings suggest that caution should be exercised while interpreting the results of Na99mTcO4 based nuclear medicine examinations in patients using J. nigra extract from green husk.",
publisher = "RAD Centre, Niš, Serbia",
journal = "RAD 2022 : 10th Jubilee International Conference on Radiation in Various Fields of Research : book of abstracts; July 25-29; Herceg Novi, Montenegro",
title = "The effect of Juglans nigra L. green husk extracts on the biodistribution of radiopharmaceutical sodium pertechnetate in mice",
pages = "119",
doi = "10.21175/rad.sum.abstr.book.2022.29.1"
}
Rajković, K., Đurašević, M. M., Milanović, Z., Vranješ-Đurić, S., Janković, D., Mirković, M.,& Obradović, Z.. (2022). The effect of Juglans nigra L. green husk extracts on the biodistribution of radiopharmaceutical sodium pertechnetate in mice. in RAD 2022 : 10th Jubilee International Conference on Radiation in Various Fields of Research : book of abstracts; July 25-29; Herceg Novi, Montenegro
RAD Centre, Niš, Serbia., 119.
https://doi.org/10.21175/rad.sum.abstr.book.2022.29.1
Rajković K, Đurašević MM, Milanović Z, Vranješ-Đurić S, Janković D, Mirković M, Obradović Z. The effect of Juglans nigra L. green husk extracts on the biodistribution of radiopharmaceutical sodium pertechnetate in mice. in RAD 2022 : 10th Jubilee International Conference on Radiation in Various Fields of Research : book of abstracts; July 25-29; Herceg Novi, Montenegro. 2022;:119.
doi:10.21175/rad.sum.abstr.book.2022.29.1 .
Rajković, Katarina, Đurašević, Mirjana M., Milanović, Zorana, Vranješ-Đurić, Sanja, Janković, Drina, Mirković, Marija, Obradović, Zorica, "The effect of Juglans nigra L. green husk extracts on the biodistribution of radiopharmaceutical sodium pertechnetate in mice" in RAD 2022 : 10th Jubilee International Conference on Radiation in Various Fields of Research : book of abstracts; July 25-29; Herceg Novi, Montenegro (2022):119,
https://doi.org/10.21175/rad.sum.abstr.book.2022.29.1 . .

Glucosomes: Magnetically induced controlled release of glucose modified liposomes

Cvjetinović, Đorđe; Milanović, Zorana; Mirković, Marija; Petrović, Jelena D.; Vesković, Ana; Popović-Bijelić, Ana; Janković, Drina; Vranješ-Đurić, Sanja

(Belgrade : Institute of Technical Sciences of SASA, 2022)

TY  - CONF
AU  - Cvjetinović, Đorđe
AU  - Milanović, Zorana
AU  - Mirković, Marija
AU  - Petrović, Jelena D.
AU  - Vesković, Ana
AU  - Popović-Bijelić, Ana
AU  - Janković, Drina
AU  - Vranješ-Đurić, Sanja
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12661
AB  - Novel methods of cancer therapy are constantly being investigated since the current approach heavily relies on the use of non-specific and toxic chemotherapy agents. Ideally, a drug used for cancer therapy would specifically target tumor sites or rather bind specifically with cancer cells. The way to achieve this is by targeting cancer cell specific receptors or receptors present in abnormally high counts at the surface. Rapid proliferation of cancer cells is fueled by large amounts of energy that is in turn produced by abnormal glucose uptake. Because of this high energy/glucose demand, cancer cells exhibit an abnormally high glucose receptor (GLUTs) count on their surface, compared to normal, healthy cells. We have utilized this glucose dependency to create glucose modified liposomes (Glucosomes) that are specifically bound by cancer cells. Glucosomes can be used to transport different substances, either hydrophilic or hydrophobic, and can therefore deliver any type of drug to cancer cells, increasing its efficiency. Another important aspect to consider is the controlled release of the drug being transported in order to maximize therapeutic efficiency. Controlled release can be achieved by utilizing different internal or external influences. In our study, we have used standard Fe3O4 magnetic nanoparticles to load glucosomes and induce their controlled opening via an external magnetic field. By applying an external magnetic field, the magnetic nanoparticles start heating up and transferring this thermal energy to the surrounding lipid bilayer, causing its perturbation and opening of the glucosome. Our study has found that controlled release can be achieved with high efficiency while the chemical stability of the Fe3O4 nanoparticles stays practically intact. Using EPR spectroscopy, we have shown that Fe3O4 nanoparticles remain trapped within the lipid bilayer and are essentially protected from oxidation that would diminish their magnetic properties. Since magnetic Fe3O4 nanoparticles are lodged well within the lipid bilayer no thermal damage can be caused to the drug being transported within the glucosome bilayer, making this a viable controlled release cancer targeting drug delivery system.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - 20th Young Researchers' Conference Materials Sciences and Engineering : program and the book of abstracts
T1  - Glucosomes: Magnetically induced controlled release of glucose modified liposomes
SP  - 12
EP  - 12
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12661
ER  - 
@conference{
author = "Cvjetinović, Đorđe and Milanović, Zorana and Mirković, Marija and Petrović, Jelena D. and Vesković, Ana and Popović-Bijelić, Ana and Janković, Drina and Vranješ-Đurić, Sanja",
year = "2022",
abstract = "Novel methods of cancer therapy are constantly being investigated since the current approach heavily relies on the use of non-specific and toxic chemotherapy agents. Ideally, a drug used for cancer therapy would specifically target tumor sites or rather bind specifically with cancer cells. The way to achieve this is by targeting cancer cell specific receptors or receptors present in abnormally high counts at the surface. Rapid proliferation of cancer cells is fueled by large amounts of energy that is in turn produced by abnormal glucose uptake. Because of this high energy/glucose demand, cancer cells exhibit an abnormally high glucose receptor (GLUTs) count on their surface, compared to normal, healthy cells. We have utilized this glucose dependency to create glucose modified liposomes (Glucosomes) that are specifically bound by cancer cells. Glucosomes can be used to transport different substances, either hydrophilic or hydrophobic, and can therefore deliver any type of drug to cancer cells, increasing its efficiency. Another important aspect to consider is the controlled release of the drug being transported in order to maximize therapeutic efficiency. Controlled release can be achieved by utilizing different internal or external influences. In our study, we have used standard Fe3O4 magnetic nanoparticles to load glucosomes and induce their controlled opening via an external magnetic field. By applying an external magnetic field, the magnetic nanoparticles start heating up and transferring this thermal energy to the surrounding lipid bilayer, causing its perturbation and opening of the glucosome. Our study has found that controlled release can be achieved with high efficiency while the chemical stability of the Fe3O4 nanoparticles stays practically intact. Using EPR spectroscopy, we have shown that Fe3O4 nanoparticles remain trapped within the lipid bilayer and are essentially protected from oxidation that would diminish their magnetic properties. Since magnetic Fe3O4 nanoparticles are lodged well within the lipid bilayer no thermal damage can be caused to the drug being transported within the glucosome bilayer, making this a viable controlled release cancer targeting drug delivery system.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "20th Young Researchers' Conference Materials Sciences and Engineering : program and the book of abstracts",
title = "Glucosomes: Magnetically induced controlled release of glucose modified liposomes",
pages = "12-12",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12661"
}
Cvjetinović, Đ., Milanović, Z., Mirković, M., Petrović, J. D., Vesković, A., Popović-Bijelić, A., Janković, D.,& Vranješ-Đurić, S.. (2022). Glucosomes: Magnetically induced controlled release of glucose modified liposomes. in 20th Young Researchers' Conference Materials Sciences and Engineering : program and the book of abstracts
Belgrade : Institute of Technical Sciences of SASA., 12-12.
https://hdl.handle.net/21.15107/rcub_vinar_12661
Cvjetinović Đ, Milanović Z, Mirković M, Petrović JD, Vesković A, Popović-Bijelić A, Janković D, Vranješ-Đurić S. Glucosomes: Magnetically induced controlled release of glucose modified liposomes. in 20th Young Researchers' Conference Materials Sciences and Engineering : program and the book of abstracts. 2022;:12-12.
https://hdl.handle.net/21.15107/rcub_vinar_12661 .
Cvjetinović, Đorđe, Milanović, Zorana, Mirković, Marija, Petrović, Jelena D., Vesković, Ana, Popović-Bijelić, Ana, Janković, Drina, Vranješ-Đurić, Sanja, "Glucosomes: Magnetically induced controlled release of glucose modified liposomes" in 20th Young Researchers' Conference Materials Sciences and Engineering : program and the book of abstracts (2022):12-12,
https://hdl.handle.net/21.15107/rcub_vinar_12661 .

Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity

Mirković, Marija D.; Radović, Magdalena; Stanković, Dalibor M.; Vranješ-Đurić, Sanja; Janković, Drina; Petrović, Djordje; Mihajlović-Lalić, Ljiljana E.; Prijović, Željko; Milanović, Zorana

(2022)

TY  - JOUR
AU  - Mirković, Marija D.
AU  - Radović, Magdalena
AU  - Stanković, Dalibor M.
AU  - Vranješ-Đurić, Sanja
AU  - Janković, Drina
AU  - Petrović, Djordje
AU  - Mihajlović-Lalić, Ljiljana E.
AU  - Prijović, Željko
AU  - Milanović, Zorana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10169
AB  - Two new Schiff base cobalt(III) ([Co(LH)Cl2], 1) and nickel(II) ([Ni(LH)ClO4], 2) complexes with a diimine-dioxime ligand, (4,9-diaza-3,10-diethyl-3,9-dodecadiene-2,11-dione bis oxime (LH2)), were synthesized and characterized. The compounds were obtained in MeOH from corresponding metal salts and LH2 in molar ratio 1:1 and further characterized by mass spectrometry, IR spectroscopy, electrochemistry, and elemental analysis. Previously reported copper(II) analog ([Cu2(LH)2]·(ClO4)2, 3) was joined to 1 and 2, and the three metal analogs, 1–3, were further investigated in terms of their electrochemical behavior. Binding studies of the complexes with deoxyribonucleic acid (DNA) and human serum albumin (HSA) were carried out using both spectrophotometric and electrochemical methods. All three complexes exhibit binding affinity towards the DNA chain through intercalative interactions. The binding reaction with HSA showed for 1 and 3 decrease in the peak current obtained in the case of complexes before the addition of HSA, while the Ni complex–HSA possesses the same electroactivity as starting complex. The cytotoxicity of LH2 as well as its metal complexes, and cisplatin were evaluated on CT-26 mouse colon carcinoma and human LS174T cancer cell lines employing MTT assay. The copper(II) complex exhibited very promising anticancer activity compared with cisplatin.
T2  - Journal of Coordination Chemistry
T1  - Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity
IS  - 1-2
SP  - 211
EP  - 224
DO  - 10.1080/00958972.2022.2032683
ER  - 
@article{
author = "Mirković, Marija D. and Radović, Magdalena and Stanković, Dalibor M. and Vranješ-Đurić, Sanja and Janković, Drina and Petrović, Djordje and Mihajlović-Lalić, Ljiljana E. and Prijović, Željko and Milanović, Zorana",
year = "2022",
abstract = "Two new Schiff base cobalt(III) ([Co(LH)Cl2], 1) and nickel(II) ([Ni(LH)ClO4], 2) complexes with a diimine-dioxime ligand, (4,9-diaza-3,10-diethyl-3,9-dodecadiene-2,11-dione bis oxime (LH2)), were synthesized and characterized. The compounds were obtained in MeOH from corresponding metal salts and LH2 in molar ratio 1:1 and further characterized by mass spectrometry, IR spectroscopy, electrochemistry, and elemental analysis. Previously reported copper(II) analog ([Cu2(LH)2]·(ClO4)2, 3) was joined to 1 and 2, and the three metal analogs, 1–3, were further investigated in terms of their electrochemical behavior. Binding studies of the complexes with deoxyribonucleic acid (DNA) and human serum albumin (HSA) were carried out using both spectrophotometric and electrochemical methods. All three complexes exhibit binding affinity towards the DNA chain through intercalative interactions. The binding reaction with HSA showed for 1 and 3 decrease in the peak current obtained in the case of complexes before the addition of HSA, while the Ni complex–HSA possesses the same electroactivity as starting complex. The cytotoxicity of LH2 as well as its metal complexes, and cisplatin were evaluated on CT-26 mouse colon carcinoma and human LS174T cancer cell lines employing MTT assay. The copper(II) complex exhibited very promising anticancer activity compared with cisplatin.",
journal = "Journal of Coordination Chemistry",
title = "Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity",
number = "1-2",
pages = "211-224",
doi = "10.1080/00958972.2022.2032683"
}
Mirković, M. D., Radović, M., Stanković, D. M., Vranješ-Đurić, S., Janković, D., Petrović, D., Mihajlović-Lalić, L. E., Prijović, Ž.,& Milanović, Z.. (2022). Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity. in Journal of Coordination Chemistry(1-2), 211-224.
https://doi.org/10.1080/00958972.2022.2032683
Mirković MD, Radović M, Stanković DM, Vranješ-Đurić S, Janković D, Petrović D, Mihajlović-Lalić LE, Prijović Ž, Milanović Z. Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity. in Journal of Coordination Chemistry. 2022;(1-2):211-224.
doi:10.1080/00958972.2022.2032683 .
Mirković, Marija D., Radović, Magdalena, Stanković, Dalibor M., Vranješ-Đurić, Sanja, Janković, Drina, Petrović, Djordje, Mihajlović-Lalić, Ljiljana E., Prijović, Željko, Milanović, Zorana, "Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity" in Journal of Coordination Chemistry, no. 1-2 (2022):211-224,
https://doi.org/10.1080/00958972.2022.2032683 . .
1
1

Transmittance Measurements in Non-alternating Magnetic Field as Reliable Method for Determining of Heating Properties of Phosphate and Phosphonate Coated Fe3O4 Magnetic Nanoparticles

Radović, Magdalena; Mirković, Marija D.; Nikolić, Aleksandar S.; Kuraica, Milorad M.; Iskrenović, Predrag; Milanović, Zorana; Vranješ-Đurić, Sanja; Perić, Marko

(2021)

TY  - JOUR
AU  - Radović, Magdalena
AU  - Mirković, Marija D.
AU  - Nikolić, Aleksandar S.
AU  - Kuraica, Milorad M.
AU  - Iskrenović, Predrag
AU  - Milanović, Zorana
AU  - Vranješ-Đurić, Sanja
AU  - Perić, Marko
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9850
AB  - Different phosphates and phosphonates have shown excellent coating ability toward magnetic nanoparticles, improving their stability and biocompatibility which enables their biomedical application. The magnetic hyperthermia efficiency of phosphates (IDP and IHP) and phosphonates (MDP and HEDP) coated Fe3O4 magnetic nanoparticles (MNPs) were evaluated in an alternating magnetic field. For a deeper understanding of hyperthermia, the behavior of investigated MNPs in the non-alternating magnetic field was monitored by measuring the transparency of the sample. To investigate their theranostic potential coated Fe3O4-MNPs were radiolabeled with radionuclide 177Lu. Phosphate coated MNPs were radiolabeled in high radiolabeling yield (&gt; 99%) while phosphonate coated MNPs reached maximum radiolabeling yield of 78%. Regardless lower radiolabeling yield both radiolabeled phosphonate MNPs may be further purified reaching radiochemical purity of more than 95%. In vitro stabile radiolabeled nanoparticles in saline and HSA were obtained. The high heating ability of phosphates and phosphonates coated MNPs as sine qua non for efficient in vivo hyperthermia treatment and satisfactory radiolabeling yield justifies their further research in order to develop new theranostic agents. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Journal of Inorganic and Organometallic Polymers and Materials
T1  - Transmittance Measurements in Non-alternating Magnetic Field as Reliable Method for Determining of Heating Properties of Phosphate and Phosphonate Coated Fe3O4 Magnetic Nanoparticles
DO  - 10.1007/s10904-021-02059-1
ER  - 
@article{
author = "Radović, Magdalena and Mirković, Marija D. and Nikolić, Aleksandar S. and Kuraica, Milorad M. and Iskrenović, Predrag and Milanović, Zorana and Vranješ-Đurić, Sanja and Perić, Marko",
year = "2021",
abstract = "Different phosphates and phosphonates have shown excellent coating ability toward magnetic nanoparticles, improving their stability and biocompatibility which enables their biomedical application. The magnetic hyperthermia efficiency of phosphates (IDP and IHP) and phosphonates (MDP and HEDP) coated Fe3O4 magnetic nanoparticles (MNPs) were evaluated in an alternating magnetic field. For a deeper understanding of hyperthermia, the behavior of investigated MNPs in the non-alternating magnetic field was monitored by measuring the transparency of the sample. To investigate their theranostic potential coated Fe3O4-MNPs were radiolabeled with radionuclide 177Lu. Phosphate coated MNPs were radiolabeled in high radiolabeling yield (&gt; 99%) while phosphonate coated MNPs reached maximum radiolabeling yield of 78%. Regardless lower radiolabeling yield both radiolabeled phosphonate MNPs may be further purified reaching radiochemical purity of more than 95%. In vitro stabile radiolabeled nanoparticles in saline and HSA were obtained. The high heating ability of phosphates and phosphonates coated MNPs as sine qua non for efficient in vivo hyperthermia treatment and satisfactory radiolabeling yield justifies their further research in order to develop new theranostic agents. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Journal of Inorganic and Organometallic Polymers and Materials",
title = "Transmittance Measurements in Non-alternating Magnetic Field as Reliable Method for Determining of Heating Properties of Phosphate and Phosphonate Coated Fe3O4 Magnetic Nanoparticles",
doi = "10.1007/s10904-021-02059-1"
}
Radović, M., Mirković, M. D., Nikolić, A. S., Kuraica, M. M., Iskrenović, P., Milanović, Z., Vranješ-Đurić, S.,& Perić, M.. (2021). Transmittance Measurements in Non-alternating Magnetic Field as Reliable Method for Determining of Heating Properties of Phosphate and Phosphonate Coated Fe3O4 Magnetic Nanoparticles. in Journal of Inorganic and Organometallic Polymers and Materials.
https://doi.org/10.1007/s10904-021-02059-1
Radović M, Mirković MD, Nikolić AS, Kuraica MM, Iskrenović P, Milanović Z, Vranješ-Đurić S, Perić M. Transmittance Measurements in Non-alternating Magnetic Field as Reliable Method for Determining of Heating Properties of Phosphate and Phosphonate Coated Fe3O4 Magnetic Nanoparticles. in Journal of Inorganic and Organometallic Polymers and Materials. 2021;.
doi:10.1007/s10904-021-02059-1 .
Radović, Magdalena, Mirković, Marija D., Nikolić, Aleksandar S., Kuraica, Milorad M., Iskrenović, Predrag, Milanović, Zorana, Vranješ-Đurić, Sanja, Perić, Marko, "Transmittance Measurements in Non-alternating Magnetic Field as Reliable Method for Determining of Heating Properties of Phosphate and Phosphonate Coated Fe3O4 Magnetic Nanoparticles" in Journal of Inorganic and Organometallic Polymers and Materials (2021),
https://doi.org/10.1007/s10904-021-02059-1 . .
3
3

177Lu-doxycycline as potential radiopharmaceutical: electrochemical characterization, radiolabeling, and biodistribution in tumor-bearing mice

Milanović, Zorana; Janković, Drina; Vranješ-Đurić, Sanja; Radović, Magdalena; Prijović, Željko; Zavišić, Gordana; Perić, Marko; Stanković, Dalibor M.; Mirković, Marija D.

(2021)

TY  - JOUR
AU  - Milanović, Zorana
AU  - Janković, Drina
AU  - Vranješ-Đurić, Sanja
AU  - Radović, Magdalena
AU  - Prijović, Željko
AU  - Zavišić, Gordana
AU  - Perić, Marko
AU  - Stanković, Dalibor M.
AU  - Mirković, Marija D.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9960
AB  - Purpose Recent studies with doxycycline as adjuvant therapy to conventional chemotherapy have shown promising results in cancer therapy. The current study aimed to examine the capability of 177Lu-labeled tetracycline ligand, doxycycline hyclate, to use as an anticancer agent.Materials and methods Doxycycline was radiolabeled with beta-emitting radioisotope 177Lu. Complex formation and its interaction with DNA were investigated electrochemically. Binding of 177Lu-doxycycline to CT 26 cell line was done. Biodistribution of 177Lu-doxycycline was examined in healthy Wistar rats and CT26 colon carcinoma tumor-bearing mice by i.v. and i.p. administration, respectively.Results Doxycycline hyclate was successfully radiolabeled with 177Lu in high radiolabeling yield (>99%). The radiolabeled complex was stable in vitro in saline and human serum over 72 h. Non-radioactive Lu-doxycycline complex formation was demonstrated electrochemically as well. Intercalative interactions of the doxycycline and Lu-doxycycline with DNA were proved using simultaneously spectrophotometric and electrochemical methods. The binding of the radiolabeled complex with plasma proteins was 4.0 ± 0.4%. The partition coefficient showed the lipophilic nature of the complex similar to the free ligand. The binding curve demonstrates binding from 0.1 nM concentrations of 177Lu-doxycycline, with half-binding estimated ∼100 nM. Biodistribution studies of 177Lu-doxycycline in CT26 colon tumor-bearing mice showed a satisfactory accumulation rate in the tumor (2.88 ± 0.85% ID/g) 3 h after intraperitoneal injection. Both the hepatobiliary system and the urinary system were prominent as excretory routes of the radiolabeled complex.Conclusion Considering obtained results, 177Lu-doxycycline complex, due to its excellent electrochemical and biological characteristics, with emphasis on the binding ability to DNA via intercalative interaction as well as significant accumulation in the tumor, is suitable for further in vivo studies to investigate its potential use in cancer treatment.
T2  - International Journal of Radiation Biology
T1  - 177Lu-doxycycline as potential radiopharmaceutical: electrochemical characterization, radiolabeling, and biodistribution in tumor-bearing mice
SP  - 1
EP  - 9
DO  - 10.1080/09553002.2021.1976864
ER  - 
@article{
author = "Milanović, Zorana and Janković, Drina and Vranješ-Đurić, Sanja and Radović, Magdalena and Prijović, Željko and Zavišić, Gordana and Perić, Marko and Stanković, Dalibor M. and Mirković, Marija D.",
year = "2021",
abstract = "Purpose Recent studies with doxycycline as adjuvant therapy to conventional chemotherapy have shown promising results in cancer therapy. The current study aimed to examine the capability of 177Lu-labeled tetracycline ligand, doxycycline hyclate, to use as an anticancer agent.Materials and methods Doxycycline was radiolabeled with beta-emitting radioisotope 177Lu. Complex formation and its interaction with DNA were investigated electrochemically. Binding of 177Lu-doxycycline to CT 26 cell line was done. Biodistribution of 177Lu-doxycycline was examined in healthy Wistar rats and CT26 colon carcinoma tumor-bearing mice by i.v. and i.p. administration, respectively.Results Doxycycline hyclate was successfully radiolabeled with 177Lu in high radiolabeling yield (>99%). The radiolabeled complex was stable in vitro in saline and human serum over 72 h. Non-radioactive Lu-doxycycline complex formation was demonstrated electrochemically as well. Intercalative interactions of the doxycycline and Lu-doxycycline with DNA were proved using simultaneously spectrophotometric and electrochemical methods. The binding of the radiolabeled complex with plasma proteins was 4.0 ± 0.4%. The partition coefficient showed the lipophilic nature of the complex similar to the free ligand. The binding curve demonstrates binding from 0.1 nM concentrations of 177Lu-doxycycline, with half-binding estimated ∼100 nM. Biodistribution studies of 177Lu-doxycycline in CT26 colon tumor-bearing mice showed a satisfactory accumulation rate in the tumor (2.88 ± 0.85% ID/g) 3 h after intraperitoneal injection. Both the hepatobiliary system and the urinary system were prominent as excretory routes of the radiolabeled complex.Conclusion Considering obtained results, 177Lu-doxycycline complex, due to its excellent electrochemical and biological characteristics, with emphasis on the binding ability to DNA via intercalative interaction as well as significant accumulation in the tumor, is suitable for further in vivo studies to investigate its potential use in cancer treatment.",
journal = "International Journal of Radiation Biology",
title = "177Lu-doxycycline as potential radiopharmaceutical: electrochemical characterization, radiolabeling, and biodistribution in tumor-bearing mice",
pages = "1-9",
doi = "10.1080/09553002.2021.1976864"
}
Milanović, Z., Janković, D., Vranješ-Đurić, S., Radović, M., Prijović, Ž., Zavišić, G., Perić, M., Stanković, D. M.,& Mirković, M. D.. (2021). 177Lu-doxycycline as potential radiopharmaceutical: electrochemical characterization, radiolabeling, and biodistribution in tumor-bearing mice. in International Journal of Radiation Biology, 1-9.
https://doi.org/10.1080/09553002.2021.1976864
Milanović Z, Janković D, Vranješ-Đurić S, Radović M, Prijović Ž, Zavišić G, Perić M, Stanković DM, Mirković MD. 177Lu-doxycycline as potential radiopharmaceutical: electrochemical characterization, radiolabeling, and biodistribution in tumor-bearing mice. in International Journal of Radiation Biology. 2021;:1-9.
doi:10.1080/09553002.2021.1976864 .
Milanović, Zorana, Janković, Drina, Vranješ-Đurić, Sanja, Radović, Magdalena, Prijović, Željko, Zavišić, Gordana, Perić, Marko, Stanković, Dalibor M., Mirković, Marija D., "177Lu-doxycycline as potential radiopharmaceutical: electrochemical characterization, radiolabeling, and biodistribution in tumor-bearing mice" in International Journal of Radiation Biology (2021):1-9,
https://doi.org/10.1080/09553002.2021.1976864 . .
1

177Lu–labeled micro liposomes as a potential radiosynoviorthesis therapeutic agent

Cvjetinović, Đorđe; Janković, Drina; Milanović, Zorana; Mirković, Marija D.; Petrović, Jelena; Prijović, Željko; Poghosyan, Emiliya; Vranješ-Đurić, Sanja

(2021)

TY  - JOUR
AU  - Cvjetinović, Đorđe
AU  - Janković, Drina
AU  - Milanović, Zorana
AU  - Mirković, Marija D.
AU  - Petrović, Jelena
AU  - Prijović, Željko
AU  - Poghosyan, Emiliya
AU  - Vranješ-Đurić, Sanja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9946
AB  - Micro–sized multivesicular liposomes were prepared, radiolabeled with 177Lu, and tested in vitro and in vivo to evaluate the potential of 177Lu–labeled micro liposomes in radiosynoviorthesis (RSO) therapy. A standard reverse–phase procedure of liposome preparation with a lipid mixture of DPPC: CHOL (80:20%) was used for the synthesis. TEM and fluorescence microscopy imaging were performed to determine the size, shape, and structure of the prepared liposomes. Both measurements are in good agreement while TEM micrographs additionally indicate to a large multivesicular inner structure of prepared liposomes. A simple and straightforward procedure was used for liposome radiolabeling with 177Lu, a well–known and commonly used radionuclide in radiotherapy with favorable properties, that can be exploited in RSO therapy. Radiolabeled 177Lu–liposomes were tested in vitro for stability and then injected into the knee joints of Wistar rats where liposome in vivo behavior was followed up to 30 days post injection. Results from both ex vivo biodistribution and in vivo imaging studies presented a high stability and retention (>94 %ID) of 177Lu–micro liposomes in the synovial liquid for the entire observation period. Leakage of free 177Lu or 177Lu–liposomes from the synovial fluid has not been detected, indicating to a possible application of 177Lu–liposomes in radiosynoviorthesis (RSO) therapy.
T2  - International Journal of Pharmaceutics
T2  - International Journal of PharmaceuticsInternational Journal of Pharmaceutics
T1  - 177Lu–labeled micro liposomes as a potential radiosynoviorthesis therapeutic agent
VL  - 608
SP  - 121106
DO  - 10.1016/j.ijpharm.2021.121106
ER  - 
@article{
author = "Cvjetinović, Đorđe and Janković, Drina and Milanović, Zorana and Mirković, Marija D. and Petrović, Jelena and Prijović, Željko and Poghosyan, Emiliya and Vranješ-Đurić, Sanja",
year = "2021",
abstract = "Micro–sized multivesicular liposomes were prepared, radiolabeled with 177Lu, and tested in vitro and in vivo to evaluate the potential of 177Lu–labeled micro liposomes in radiosynoviorthesis (RSO) therapy. A standard reverse–phase procedure of liposome preparation with a lipid mixture of DPPC: CHOL (80:20%) was used for the synthesis. TEM and fluorescence microscopy imaging were performed to determine the size, shape, and structure of the prepared liposomes. Both measurements are in good agreement while TEM micrographs additionally indicate to a large multivesicular inner structure of prepared liposomes. A simple and straightforward procedure was used for liposome radiolabeling with 177Lu, a well–known and commonly used radionuclide in radiotherapy with favorable properties, that can be exploited in RSO therapy. Radiolabeled 177Lu–liposomes were tested in vitro for stability and then injected into the knee joints of Wistar rats where liposome in vivo behavior was followed up to 30 days post injection. Results from both ex vivo biodistribution and in vivo imaging studies presented a high stability and retention (>94 %ID) of 177Lu–micro liposomes in the synovial liquid for the entire observation period. Leakage of free 177Lu or 177Lu–liposomes from the synovial fluid has not been detected, indicating to a possible application of 177Lu–liposomes in radiosynoviorthesis (RSO) therapy.",
journal = "International Journal of Pharmaceutics, International Journal of PharmaceuticsInternational Journal of Pharmaceutics",
title = "177Lu–labeled micro liposomes as a potential radiosynoviorthesis therapeutic agent",
volume = "608",
pages = "121106",
doi = "10.1016/j.ijpharm.2021.121106"
}
Cvjetinović, Đ., Janković, D., Milanović, Z., Mirković, M. D., Petrović, J., Prijović, Ž., Poghosyan, E.,& Vranješ-Đurić, S.. (2021). 177Lu–labeled micro liposomes as a potential radiosynoviorthesis therapeutic agent. in International Journal of Pharmaceutics, 608, 121106.
https://doi.org/10.1016/j.ijpharm.2021.121106
Cvjetinović Đ, Janković D, Milanović Z, Mirković MD, Petrović J, Prijović Ž, Poghosyan E, Vranješ-Đurić S. 177Lu–labeled micro liposomes as a potential radiosynoviorthesis therapeutic agent. in International Journal of Pharmaceutics. 2021;608:121106.
doi:10.1016/j.ijpharm.2021.121106 .
Cvjetinović, Đorđe, Janković, Drina, Milanović, Zorana, Mirković, Marija D., Petrović, Jelena, Prijović, Željko, Poghosyan, Emiliya, Vranješ-Đurić, Sanja, "177Lu–labeled micro liposomes as a potential radiosynoviorthesis therapeutic agent" in International Journal of Pharmaceutics, 608 (2021):121106,
https://doi.org/10.1016/j.ijpharm.2021.121106 . .

Magnetically induced controlled release from glucose-modified liposomes loaded with Fe3O4 nanoparticles

Cvjetinović, Đorđe; Milanović, Zorana; Mirković, Marija D.; Petrović, Jelena; Vesković, Ana; Popović-Bijelić, Ana; Prijović, Željko; Janković, Drina; Vranješ-Đurić, Sanja

(2021)

TY  - JOUR
AU  - Cvjetinović, Đorđe
AU  - Milanović, Zorana
AU  - Mirković, Marija D.
AU  - Petrović, Jelena
AU  - Vesković, Ana
AU  - Popović-Bijelić, Ana
AU  - Prijović, Željko
AU  - Janković, Drina
AU  - Vranješ-Đurić, Sanja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10063
AB  - Small glucose-modified liposomes (GMLs) were loaded with magnetic Fe3O4 nanoparticles (MNPs) and fluorescein using a standard thin layer preparation procedure and a varying lipid/MNPs ratio. The liposomes were characterized with TEM and DLS measurements, and MNPs encapsulation rate was determined using ICP-OES. Prepared liposomes were stored at 5 °C for 30 days and subsequently exposed to an external magnetic field (20 mT) with varying exposure times (2‒20 min), at room temperature. The release of fluorescein from GMLs induced by the magnetic field exposures was quantified, showing a high release rate (25‒85%) depending on the concentration of MNPs in GMLs. EPR measurements were conducted during the liposomes storage period in order to provide semi-quantitative information of possible MNPs oxidation from Fe3O4 to Fe2O3 inside the liposomes, impacting MNPs magnetic properties. In contrast to the MNPs water dispersion, no significant change in the EPR signal of MNPs encapsulated inside GMLs was detected over the course of 30 days. The data presented in this study indicate that GMLs loaded with MNPs maintain a high stability for prolonged periods of time and that this delivery system may be used for magnetically assisted controlled drug release.
T2  - Journal of Nanoparticle Research
T1  - Magnetically induced controlled release from glucose-modified liposomes loaded with Fe3O4 nanoparticles
VL  - 23
IS  - 11
SP  - 252
DO  - 10.1007/s11051-021-05375-2
ER  - 
@article{
author = "Cvjetinović, Đorđe and Milanović, Zorana and Mirković, Marija D. and Petrović, Jelena and Vesković, Ana and Popović-Bijelić, Ana and Prijović, Željko and Janković, Drina and Vranješ-Đurić, Sanja",
year = "2021",
abstract = "Small glucose-modified liposomes (GMLs) were loaded with magnetic Fe3O4 nanoparticles (MNPs) and fluorescein using a standard thin layer preparation procedure and a varying lipid/MNPs ratio. The liposomes were characterized with TEM and DLS measurements, and MNPs encapsulation rate was determined using ICP-OES. Prepared liposomes were stored at 5 °C for 30 days and subsequently exposed to an external magnetic field (20 mT) with varying exposure times (2‒20 min), at room temperature. The release of fluorescein from GMLs induced by the magnetic field exposures was quantified, showing a high release rate (25‒85%) depending on the concentration of MNPs in GMLs. EPR measurements were conducted during the liposomes storage period in order to provide semi-quantitative information of possible MNPs oxidation from Fe3O4 to Fe2O3 inside the liposomes, impacting MNPs magnetic properties. In contrast to the MNPs water dispersion, no significant change in the EPR signal of MNPs encapsulated inside GMLs was detected over the course of 30 days. The data presented in this study indicate that GMLs loaded with MNPs maintain a high stability for prolonged periods of time and that this delivery system may be used for magnetically assisted controlled drug release.",
journal = "Journal of Nanoparticle Research",
title = "Magnetically induced controlled release from glucose-modified liposomes loaded with Fe3O4 nanoparticles",
volume = "23",
number = "11",
pages = "252",
doi = "10.1007/s11051-021-05375-2"
}
Cvjetinović, Đ., Milanović, Z., Mirković, M. D., Petrović, J., Vesković, A., Popović-Bijelić, A., Prijović, Ž., Janković, D.,& Vranješ-Đurić, S.. (2021). Magnetically induced controlled release from glucose-modified liposomes loaded with Fe3O4 nanoparticles. in Journal of Nanoparticle Research, 23(11), 252.
https://doi.org/10.1007/s11051-021-05375-2
Cvjetinović Đ, Milanović Z, Mirković MD, Petrović J, Vesković A, Popović-Bijelić A, Prijović Ž, Janković D, Vranješ-Đurić S. Magnetically induced controlled release from glucose-modified liposomes loaded with Fe3O4 nanoparticles. in Journal of Nanoparticle Research. 2021;23(11):252.
doi:10.1007/s11051-021-05375-2 .
Cvjetinović, Đorđe, Milanović, Zorana, Mirković, Marija D., Petrović, Jelena, Vesković, Ana, Popović-Bijelić, Ana, Prijović, Željko, Janković, Drina, Vranješ-Đurić, Sanja, "Magnetically induced controlled release from glucose-modified liposomes loaded with Fe3O4 nanoparticles" in Journal of Nanoparticle Research, 23, no. 11 (2021):252,
https://doi.org/10.1007/s11051-021-05375-2 . .
1
1

Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking

Cvjetinović, Đorđe; Prijović, Željko; Janković, Drina; Radović, Magdalena; Mirković, Marija D.; Milanović, Zorana; Mojović, Miloš; Škalamera, Đani; Vranješ-Đurić, Sanja

(2021)

TY  - JOUR
AU  - Cvjetinović, Đorđe
AU  - Prijović, Željko
AU  - Janković, Drina
AU  - Radović, Magdalena
AU  - Mirković, Marija D.
AU  - Milanović, Zorana
AU  - Mojović, Miloš
AU  - Škalamera, Đani
AU  - Vranješ-Đurić, Sanja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9151
AB  - Liposomes are promising drug's delivery systems due to decreased toxicity of the liposome-encapsulated drug, but wider clinical application requires their more efficient tumor targeting with uptake, controlled drug release and higher shelf life. The unique metabolic characteristics of cancer cells based on higher demand for energy and therefore increased glucose utilization were exploited in the design of glucose modified liposomes (GML) with the aim to provide increased tumor targeting via glucose transporters and increased ability of drug delivery into tumor cells. Tumor accumulating potential of GML and non-glucose liposomes (NGL) were investigated on CT26 and LS174T tumor-bearing mice by simple and reliable radiotracer method using 177Lu as radioactive marker. Both liposomes, GML and NGL were radiolabeled in high radiolabeling yield, showing high in vitro stability in biological media, as the main prerequisite for the biodistribution studies. Tumors displayed significantly better accumulation of 177Lu-GML with the maximum uptake 6 h post-injection (5.8 ± 0.2%/g in LS174T tumor and 5.1 ± 0.5%/g in CT26 tumor), compared to negligible uptake of 177Lu-NGL (0.6 ± 0.1%/g in LS174T tumor and 0.9 ± 0.2%/g in CT26 tumor). Results of comparative biodistribution studies of 177Lu-NGL and 177Lu-GML indicate that increased accumulation of GML is enabled by glucose transporters and subsequent endocytosis, resulting in their prolonged retention in tumor tissues (up to 72 h). Direct radiolabeling of liposomes with 177Lu may be used not only for biodistribution studies using radiotracking, but also for cancer treatment. © 2021 Elsevier B.V.
T2  - Journal of Controlled Release
T1  - Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking
VL  - 332
SP  - 301
EP  - 311
DO  - 10.1016/j.jconrel.2021.03.006
ER  - 
@article{
author = "Cvjetinović, Đorđe and Prijović, Željko and Janković, Drina and Radović, Magdalena and Mirković, Marija D. and Milanović, Zorana and Mojović, Miloš and Škalamera, Đani and Vranješ-Đurić, Sanja",
year = "2021",
abstract = "Liposomes are promising drug's delivery systems due to decreased toxicity of the liposome-encapsulated drug, but wider clinical application requires their more efficient tumor targeting with uptake, controlled drug release and higher shelf life. The unique metabolic characteristics of cancer cells based on higher demand for energy and therefore increased glucose utilization were exploited in the design of glucose modified liposomes (GML) with the aim to provide increased tumor targeting via glucose transporters and increased ability of drug delivery into tumor cells. Tumor accumulating potential of GML and non-glucose liposomes (NGL) were investigated on CT26 and LS174T tumor-bearing mice by simple and reliable radiotracer method using 177Lu as radioactive marker. Both liposomes, GML and NGL were radiolabeled in high radiolabeling yield, showing high in vitro stability in biological media, as the main prerequisite for the biodistribution studies. Tumors displayed significantly better accumulation of 177Lu-GML with the maximum uptake 6 h post-injection (5.8 ± 0.2%/g in LS174T tumor and 5.1 ± 0.5%/g in CT26 tumor), compared to negligible uptake of 177Lu-NGL (0.6 ± 0.1%/g in LS174T tumor and 0.9 ± 0.2%/g in CT26 tumor). Results of comparative biodistribution studies of 177Lu-NGL and 177Lu-GML indicate that increased accumulation of GML is enabled by glucose transporters and subsequent endocytosis, resulting in their prolonged retention in tumor tissues (up to 72 h). Direct radiolabeling of liposomes with 177Lu may be used not only for biodistribution studies using radiotracking, but also for cancer treatment. © 2021 Elsevier B.V.",
journal = "Journal of Controlled Release",
title = "Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking",
volume = "332",
pages = "301-311",
doi = "10.1016/j.jconrel.2021.03.006"
}
Cvjetinović, Đ., Prijović, Ž., Janković, D., Radović, M., Mirković, M. D., Milanović, Z., Mojović, M., Škalamera, Đ.,& Vranješ-Đurić, S.. (2021). Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking. in Journal of Controlled Release, 332, 301-311.
https://doi.org/10.1016/j.jconrel.2021.03.006
Cvjetinović Đ, Prijović Ž, Janković D, Radović M, Mirković MD, Milanović Z, Mojović M, Škalamera Đ, Vranješ-Đurić S. Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking. in Journal of Controlled Release. 2021;332:301-311.
doi:10.1016/j.jconrel.2021.03.006 .
Cvjetinović, Đorđe, Prijović, Željko, Janković, Drina, Radović, Magdalena, Mirković, Marija D., Milanović, Zorana, Mojović, Miloš, Škalamera, Đani, Vranješ-Đurić, Sanja, "Bioevaluation of glucose-modified liposomes as a potential drug delivery system for cancer treatment using 177-Lu radiotracking" in Journal of Controlled Release, 332 (2021):301-311,
https://doi.org/10.1016/j.jconrel.2021.03.006 . .
1
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4
20

Screen printed diamond electrode as efficient “point-of-care” platform for submicromolar determination of cytostatic drug in biological fluids and pharmaceutical product

Stanković, Dalibor M.; Milanović, Zorana; Švorc, Lubomir; Stanković, Vesna; Janković, Drina; Mirković, Marija D.; Vranješ-Đurić, Sanja

(2021)

TY  - JOUR
AU  - Stanković, Dalibor M.
AU  - Milanović, Zorana
AU  - Švorc, Lubomir
AU  - Stanković, Vesna
AU  - Janković, Drina
AU  - Mirković, Marija D.
AU  - Vranješ-Đurić, Sanja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9543
AB  - This work presents advanced electrochemical platform based on screen printed diamond electrode (SPDE) system for the single drop “point-of-care” testing. Proposed approach was applied for the quantification of doxorubicin hydrochloride (DOX) in biological fluids and pharmaceutical product. Using a single drop (~30 μL) of the tested solution at the electrode surface, DOX showed high electroactivity over a wide range of pHs. In these conditions, single oval shaped, well-defined and pH dependent oxidation peak was observed in the potential range from 0.5 V to 1.3 V. In the reverse scan, two cathodic peaks, were noted – around 0.3 V and – 0.5 V. Similarly, first reduction peak was pH dependent, while second one was independent in the studied range. Experimental conditions for DOX quantification were optimized and natures of the electrode reactions were investigated. Working linear range obtained for DOX detection was from 0.1 to 2.5 μM. Diffusion controlled electrode reaction reveal long life time of the proposed electrode as well high potential for practical application. Developed procedure was successfully applied for the DOX analysis in biological fluids – urine and pharmaceutical formulation. Obtained results clearly indicated that given procedure can be easily implemented for pharmaceutical control and medical analysis, in both, laboratory and field conditions. © 2021 Elsevier B.V.
T2  - Diamond and Related Materials
T1  - Screen printed diamond electrode as efficient “point-of-care” platform for submicromolar determination of cytostatic drug in biological fluids and pharmaceutical product
VL  - 113
SP  - 108277
DO  - 10.1016/j.diamond.2021.108277
ER  - 
@article{
author = "Stanković, Dalibor M. and Milanović, Zorana and Švorc, Lubomir and Stanković, Vesna and Janković, Drina and Mirković, Marija D. and Vranješ-Đurić, Sanja",
year = "2021",
abstract = "This work presents advanced electrochemical platform based on screen printed diamond electrode (SPDE) system for the single drop “point-of-care” testing. Proposed approach was applied for the quantification of doxorubicin hydrochloride (DOX) in biological fluids and pharmaceutical product. Using a single drop (~30 μL) of the tested solution at the electrode surface, DOX showed high electroactivity over a wide range of pHs. In these conditions, single oval shaped, well-defined and pH dependent oxidation peak was observed in the potential range from 0.5 V to 1.3 V. In the reverse scan, two cathodic peaks, were noted – around 0.3 V and – 0.5 V. Similarly, first reduction peak was pH dependent, while second one was independent in the studied range. Experimental conditions for DOX quantification were optimized and natures of the electrode reactions were investigated. Working linear range obtained for DOX detection was from 0.1 to 2.5 μM. Diffusion controlled electrode reaction reveal long life time of the proposed electrode as well high potential for practical application. Developed procedure was successfully applied for the DOX analysis in biological fluids – urine and pharmaceutical formulation. Obtained results clearly indicated that given procedure can be easily implemented for pharmaceutical control and medical analysis, in both, laboratory and field conditions. © 2021 Elsevier B.V.",
journal = "Diamond and Related Materials",
title = "Screen printed diamond electrode as efficient “point-of-care” platform for submicromolar determination of cytostatic drug in biological fluids and pharmaceutical product",
volume = "113",
pages = "108277",
doi = "10.1016/j.diamond.2021.108277"
}
Stanković, D. M., Milanović, Z., Švorc, L., Stanković, V., Janković, D., Mirković, M. D.,& Vranješ-Đurić, S.. (2021). Screen printed diamond electrode as efficient “point-of-care” platform for submicromolar determination of cytostatic drug in biological fluids and pharmaceutical product. in Diamond and Related Materials, 113, 108277.
https://doi.org/10.1016/j.diamond.2021.108277
Stanković DM, Milanović Z, Švorc L, Stanković V, Janković D, Mirković MD, Vranješ-Đurić S. Screen printed diamond electrode as efficient “point-of-care” platform for submicromolar determination of cytostatic drug in biological fluids and pharmaceutical product. in Diamond and Related Materials. 2021;113:108277.
doi:10.1016/j.diamond.2021.108277 .
Stanković, Dalibor M., Milanović, Zorana, Švorc, Lubomir, Stanković, Vesna, Janković, Drina, Mirković, Marija D., Vranješ-Đurić, Sanja, "Screen printed diamond electrode as efficient “point-of-care” platform for submicromolar determination of cytostatic drug in biological fluids and pharmaceutical product" in Diamond and Related Materials, 113 (2021):108277,
https://doi.org/10.1016/j.diamond.2021.108277 . .
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A single drop histamine sensor based on AuNPs/MnO2 modified screen-printed electrode

Knežević, Sara; Ognjanović, Miloš; Nedić, Nemanja; Mariano, Jose F. M. L.; Milanović, Zorana; Petković, Branka B.; Antić, Bratislav; Vranješ-Đurić, Sanja; Stanković, Dalibor M.

(2020)

TY  - JOUR
AU  - Knežević, Sara
AU  - Ognjanović, Miloš
AU  - Nedić, Nemanja
AU  - Mariano, Jose F. M. L.
AU  - Milanović, Zorana
AU  - Petković, Branka B.
AU  - Antić, Bratislav
AU  - Vranješ-Đurić, Sanja
AU  - Stanković, Dalibor M.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8834
AB  - In order to ensure high food quality, one of the prime importance is the detection and quantification of histamine, well known marine food poison. In this work, we constructed novel electrochemical biosensor for the detection of histamine based on gold nanoparticles decorated on manganese dioxide (Au/MnO2) and used for modification of screen-printed carbon electrode (Au/MnO2@SPCE). The constructed sensor was then used for the estimation of histamine content in a single drop. Materials used in this study were synthesized and characterized using HR-TEM, XRPD and electrochemical methods. The amperometric detection method was optimized and, under selected operating parameters (supporting electrolyte pH 6, working potential of 1 V), the proposed sensor possesses linear working range from 0.3 µM to 5.1 µM, with a detection limit of 0.08 µM. The effect of selected interferences was investigated and it was found that the developed approach offers accurate, precise, selective, fast and reproducible quantification of histamine using only one drop of the sample. In the end, this work stands as a proof-of-concept of the modified electrodes and electrochemical detection as a promising and prospective approach for the applications in real-time monitoring of the food quality.
T2  - Microchemical Journal
T1  - A single drop histamine sensor based on AuNPs/MnO2 modified screen-printed electrode
VL  - 155
SP  - 104778
DO  - 10.1016/j.microc.2020.104778
ER  - 
@article{
author = "Knežević, Sara and Ognjanović, Miloš and Nedić, Nemanja and Mariano, Jose F. M. L. and Milanović, Zorana and Petković, Branka B. and Antić, Bratislav and Vranješ-Đurić, Sanja and Stanković, Dalibor M.",
year = "2020",
abstract = "In order to ensure high food quality, one of the prime importance is the detection and quantification of histamine, well known marine food poison. In this work, we constructed novel electrochemical biosensor for the detection of histamine based on gold nanoparticles decorated on manganese dioxide (Au/MnO2) and used for modification of screen-printed carbon electrode (Au/MnO2@SPCE). The constructed sensor was then used for the estimation of histamine content in a single drop. Materials used in this study were synthesized and characterized using HR-TEM, XRPD and electrochemical methods. The amperometric detection method was optimized and, under selected operating parameters (supporting electrolyte pH 6, working potential of 1 V), the proposed sensor possesses linear working range from 0.3 µM to 5.1 µM, with a detection limit of 0.08 µM. The effect of selected interferences was investigated and it was found that the developed approach offers accurate, precise, selective, fast and reproducible quantification of histamine using only one drop of the sample. In the end, this work stands as a proof-of-concept of the modified electrodes and electrochemical detection as a promising and prospective approach for the applications in real-time monitoring of the food quality.",
journal = "Microchemical Journal",
title = "A single drop histamine sensor based on AuNPs/MnO2 modified screen-printed electrode",
volume = "155",
pages = "104778",
doi = "10.1016/j.microc.2020.104778"
}
Knežević, S., Ognjanović, M., Nedić, N., Mariano, J. F. M. L., Milanović, Z., Petković, B. B., Antić, B., Vranješ-Đurić, S.,& Stanković, D. M.. (2020). A single drop histamine sensor based on AuNPs/MnO2 modified screen-printed electrode. in Microchemical Journal, 155, 104778.
https://doi.org/10.1016/j.microc.2020.104778
Knežević S, Ognjanović M, Nedić N, Mariano JFML, Milanović Z, Petković BB, Antić B, Vranješ-Đurić S, Stanković DM. A single drop histamine sensor based on AuNPs/MnO2 modified screen-printed electrode. in Microchemical Journal. 2020;155:104778.
doi:10.1016/j.microc.2020.104778 .
Knežević, Sara, Ognjanović, Miloš, Nedić, Nemanja, Mariano, Jose F. M. L., Milanović, Zorana, Petković, Branka B., Antić, Bratislav, Vranješ-Đurić, Sanja, Stanković, Dalibor M., "A single drop histamine sensor based on AuNPs/MnO2 modified screen-printed electrode" in Microchemical Journal, 155 (2020):104778,
https://doi.org/10.1016/j.microc.2020.104778 . .
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Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer

Stanković, Aljoša; Mihailović, Jasna; Mirković, Marija D.; Radović, Magdalena; Milanović, Zorana; Ognjanović, Miloš; Janković, Drina; Antić, Bratislav; Mijović, Milica; Vranješ-Đurić, Sanja; Prijović, Željko

(2020)

TY  - JOUR
AU  - Stanković, Aljoša
AU  - Mihailović, Jasna
AU  - Mirković, Marija D.
AU  - Radović, Magdalena
AU  - Milanović, Zorana
AU  - Ognjanović, Miloš
AU  - Janković, Drina
AU  - Antić, Bratislav
AU  - Mijović, Milica
AU  - Vranješ-Đurić, Sanja
AU  - Prijović, Željko
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9117
AB  - Combined radionuclide therapy with magnetic nanoparticles-mediated hyperthermia has been under research focus as a promising tumor therapy approach. The objective of this study was to investigate the potential of 131I-radiolabeled superparamagnetic iron oxide nanoparticles (SPIONs) prepared as the ~40 nm flower-shaped structures with excellent heating efficiency (specific absorption rate at H0 = 15.9 kA∙m−1 and resonant frequency of 252 kHz was 123.1 W∙g−1) for nano-brachytherapy of tumors. 131I-radiolabeled CC49 antibody attached to SPIONs via reactive groups of 3-aminopropyltriethoxysilane (APTES) provided specificity and long-lasting localized retention after their intratumoral application into LS174T human colon adenocarcinoma xenografts in NOD-SCID mice. The results demonstrate feasibility and effectiveness of magnetic hyperthermia (HT), radionuclide therapy (RT) and their combination (HT + RT) in treating cancer in xenograft models. Combined therapy approach induced a significant (p < 0.01) tumor growth suppression in comparison to untreated groups presented by the tumor volume inhibitory rate (TVIR): 54.38%, 68.77%, 73.00% for HT, RT and HT + RT, respectively in comparison to untreated group and 48.31%, 64,62% and 69,41%, respectively, for the SPIONs-only injected group. Histopathology analysis proved the necrosis and apoptosis in treated tumors without general toxicity. Obtained data support the idea that nano-brachytherapy combined with hyperthermia is a promising approach for effective cancer treatment.
T2  - International Journal of Pharmaceutics
T1  - Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer
VL  - 587
SP  - 119628
DO  - 10.1016/j.ijpharm.2020.119628
ER  - 
@article{
author = "Stanković, Aljoša and Mihailović, Jasna and Mirković, Marija D. and Radović, Magdalena and Milanović, Zorana and Ognjanović, Miloš and Janković, Drina and Antić, Bratislav and Mijović, Milica and Vranješ-Đurić, Sanja and Prijović, Željko",
year = "2020",
abstract = "Combined radionuclide therapy with magnetic nanoparticles-mediated hyperthermia has been under research focus as a promising tumor therapy approach. The objective of this study was to investigate the potential of 131I-radiolabeled superparamagnetic iron oxide nanoparticles (SPIONs) prepared as the ~40 nm flower-shaped structures with excellent heating efficiency (specific absorption rate at H0 = 15.9 kA∙m−1 and resonant frequency of 252 kHz was 123.1 W∙g−1) for nano-brachytherapy of tumors. 131I-radiolabeled CC49 antibody attached to SPIONs via reactive groups of 3-aminopropyltriethoxysilane (APTES) provided specificity and long-lasting localized retention after their intratumoral application into LS174T human colon adenocarcinoma xenografts in NOD-SCID mice. The results demonstrate feasibility and effectiveness of magnetic hyperthermia (HT), radionuclide therapy (RT) and their combination (HT + RT) in treating cancer in xenograft models. Combined therapy approach induced a significant (p < 0.01) tumor growth suppression in comparison to untreated groups presented by the tumor volume inhibitory rate (TVIR): 54.38%, 68.77%, 73.00% for HT, RT and HT + RT, respectively in comparison to untreated group and 48.31%, 64,62% and 69,41%, respectively, for the SPIONs-only injected group. Histopathology analysis proved the necrosis and apoptosis in treated tumors without general toxicity. Obtained data support the idea that nano-brachytherapy combined with hyperthermia is a promising approach for effective cancer treatment.",
journal = "International Journal of Pharmaceutics",
title = "Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer",
volume = "587",
pages = "119628",
doi = "10.1016/j.ijpharm.2020.119628"
}
Stanković, A., Mihailović, J., Mirković, M. D., Radović, M., Milanović, Z., Ognjanović, M., Janković, D., Antić, B., Mijović, M., Vranješ-Đurić, S.,& Prijović, Ž.. (2020). Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer. in International Journal of Pharmaceutics, 587, 119628.
https://doi.org/10.1016/j.ijpharm.2020.119628
Stanković A, Mihailović J, Mirković MD, Radović M, Milanović Z, Ognjanović M, Janković D, Antić B, Mijović M, Vranješ-Đurić S, Prijović Ž. Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer. in International Journal of Pharmaceutics. 2020;587:119628.
doi:10.1016/j.ijpharm.2020.119628 .
Stanković, Aljoša, Mihailović, Jasna, Mirković, Marija D., Radović, Magdalena, Milanović, Zorana, Ognjanović, Miloš, Janković, Drina, Antić, Bratislav, Mijović, Milica, Vranješ-Đurić, Sanja, Prijović, Željko, "Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer" in International Journal of Pharmaceutics, 587 (2020):119628,
https://doi.org/10.1016/j.ijpharm.2020.119628 . .
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Optimization of the radiolabelling method for improved in vitro and in vivo stability of 90Y-albumin microspheres

Vukadinović, Aleksandar; Janković, Drina; Radović, Magdalena; Milanović, Zorana; Mirković, Marija D.; Stanković, Dragana; Vranješ-Đurić, Sanja

(2020)

TY  - JOUR
AU  - Vukadinović, Aleksandar
AU  - Janković, Drina
AU  - Radović, Magdalena
AU  - Milanović, Zorana
AU  - Mirković, Marija D.
AU  - Stanković, Dragana
AU  - Vranješ-Đurić, Sanja
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8652
AB  - Biologically stable 90Y-labelled albumin microspheres (AMS) were developed by optimizing the process of their preparation. Three formulations of 90Y-AMS were initially prepared with high radiolabelling yield but depending on the step when the radionuclide 90Y and DTPA chelator were added, radiolabelled microspheres with different in vitro and in vivo stability were obtained. DTPA was proved as a useful chelating agent that tightly links radionuclide 90Y to albumin. Also, AMS radiolabelled via DTPA during preparation and before microspheres stabilization, showed significant in vitro and in vivo stability ready for the potential use in selective internal radiation therapy. © 2019 Elsevier Ltd
T2  - Applied Radiation and Isotopes
T1  - Optimization of the radiolabelling method for improved in vitro and in vivo stability of 90Y-albumin microspheres
VL  - 156
SP  - 108984
DO  - 10.1016/j.apradiso.2019.108984
ER  - 
@article{
author = "Vukadinović, Aleksandar and Janković, Drina and Radović, Magdalena and Milanović, Zorana and Mirković, Marija D. and Stanković, Dragana and Vranješ-Đurić, Sanja",
year = "2020",
abstract = "Biologically stable 90Y-labelled albumin microspheres (AMS) were developed by optimizing the process of their preparation. Three formulations of 90Y-AMS were initially prepared with high radiolabelling yield but depending on the step when the radionuclide 90Y and DTPA chelator were added, radiolabelled microspheres with different in vitro and in vivo stability were obtained. DTPA was proved as a useful chelating agent that tightly links radionuclide 90Y to albumin. Also, AMS radiolabelled via DTPA during preparation and before microspheres stabilization, showed significant in vitro and in vivo stability ready for the potential use in selective internal radiation therapy. © 2019 Elsevier Ltd",
journal = "Applied Radiation and Isotopes",
title = "Optimization of the radiolabelling method for improved in vitro and in vivo stability of 90Y-albumin microspheres",
volume = "156",
pages = "108984",
doi = "10.1016/j.apradiso.2019.108984"
}
Vukadinović, A., Janković, D., Radović, M., Milanović, Z., Mirković, M. D., Stanković, D.,& Vranješ-Đurić, S.. (2020). Optimization of the radiolabelling method for improved in vitro and in vivo stability of 90Y-albumin microspheres. in Applied Radiation and Isotopes, 156, 108984.
https://doi.org/10.1016/j.apradiso.2019.108984
Vukadinović A, Janković D, Radović M, Milanović Z, Mirković MD, Stanković D, Vranješ-Đurić S. Optimization of the radiolabelling method for improved in vitro and in vivo stability of 90Y-albumin microspheres. in Applied Radiation and Isotopes. 2020;156:108984.
doi:10.1016/j.apradiso.2019.108984 .
Vukadinović, Aleksandar, Janković, Drina, Radović, Magdalena, Milanović, Zorana, Mirković, Marija D., Stanković, Dragana, Vranješ-Đurić, Sanja, "Optimization of the radiolabelling method for improved in vitro and in vivo stability of 90Y-albumin microspheres" in Applied Radiation and Isotopes, 156 (2020):108984,
https://doi.org/10.1016/j.apradiso.2019.108984 . .
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Investigation of 177Lu-labeled HEDP, DPD, and IDP as potential bone pain palliation agents

Mirković, Marija D.; Milanović, Zorana; Stanković, Dalibor M.; Petrović, Đorđe; Vranješ-Đurić, Sanja; Janković, Drina; Radović, Magdalena

(2020)

TY  - JOUR
AU  - Mirković, Marija D.
AU  - Milanović, Zorana
AU  - Stanković, Dalibor M.
AU  - Petrović, Đorđe
AU  - Vranješ-Đurić, Sanja
AU  - Janković, Drina
AU  - Radović, Magdalena
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10698
AB  - Application of bone-seeking radiopharmaceuticals is one of the modalities in the management of metastatic bone pain. The present study aimed to investigate the potential of Lu-177-labeled phosphate/phosphonate ligands: 1-hydroxyethane 1,1-diphosphonic acid (HEDP), 2,3-dicarboxypropane-1,1-diphosphonic acid (DPD), and imidodiphosphate tetrasodium salt (IDP), as bone pain palliation agents. HEDP, DPD, and IDP were radiolabeled with Lu-177 in high radiolabeling yield (98.49%, 93.31%, and 90.69%, respectively), forming in vitro stable radiolabeled complexes in saline and human serum after 96 h. Biodistribution was followed by imaging studies and ex vivo measurement of radioactivity in organs in healthy Wistar rats. Significant bone accumulation and long retention even after 96 h (3.85 +/- 0.91%ID/g), as well as relatively low uptake in soft tissue such as liver and spleen (<1%ID/g), were observed for Lu-177-HEDP. Two other radiolabeled ligands showed lower accumulations in bone (<1% ID/g) and higher accumulations in liver and spleen at examined time points (>1.5% ID/g). Obtained results suggest that difference in the chemical structure of phosphonates/phosphates influences the rate of bone incorporation of Lu-177-radiolabeled complexes. Desirable biodistribution pattern of Lu-177-HEDP makes it suitable for its further preclinical investigations as a potential bone pain palliation agent.
T2  - Journal of Radiation Research and Applied Sciences
T1  - Investigation of 177Lu-labeled HEDP, DPD, and IDP as potential bone pain palliation agents
VL  - 13
IS  - 1
SP  - 27
EP  - 36
DO  - 10.1080/16878507.2019.1702243
ER  - 
@article{
author = "Mirković, Marija D. and Milanović, Zorana and Stanković, Dalibor M. and Petrović, Đorđe and Vranješ-Đurić, Sanja and Janković, Drina and Radović, Magdalena",
year = "2020",
abstract = "Application of bone-seeking radiopharmaceuticals is one of the modalities in the management of metastatic bone pain. The present study aimed to investigate the potential of Lu-177-labeled phosphate/phosphonate ligands: 1-hydroxyethane 1,1-diphosphonic acid (HEDP), 2,3-dicarboxypropane-1,1-diphosphonic acid (DPD), and imidodiphosphate tetrasodium salt (IDP), as bone pain palliation agents. HEDP, DPD, and IDP were radiolabeled with Lu-177 in high radiolabeling yield (98.49%, 93.31%, and 90.69%, respectively), forming in vitro stable radiolabeled complexes in saline and human serum after 96 h. Biodistribution was followed by imaging studies and ex vivo measurement of radioactivity in organs in healthy Wistar rats. Significant bone accumulation and long retention even after 96 h (3.85 +/- 0.91%ID/g), as well as relatively low uptake in soft tissue such as liver and spleen (<1%ID/g), were observed for Lu-177-HEDP. Two other radiolabeled ligands showed lower accumulations in bone (<1% ID/g) and higher accumulations in liver and spleen at examined time points (>1.5% ID/g). Obtained results suggest that difference in the chemical structure of phosphonates/phosphates influences the rate of bone incorporation of Lu-177-radiolabeled complexes. Desirable biodistribution pattern of Lu-177-HEDP makes it suitable for its further preclinical investigations as a potential bone pain palliation agent.",
journal = "Journal of Radiation Research and Applied Sciences",
title = "Investigation of 177Lu-labeled HEDP, DPD, and IDP as potential bone pain palliation agents",
volume = "13",
number = "1",
pages = "27-36",
doi = "10.1080/16878507.2019.1702243"
}
Mirković, M. D., Milanović, Z., Stanković, D. M., Petrović, Đ., Vranješ-Đurić, S., Janković, D.,& Radović, M.. (2020). Investigation of 177Lu-labeled HEDP, DPD, and IDP as potential bone pain palliation agents. in Journal of Radiation Research and Applied Sciences, 13(1), 27-36.
https://doi.org/10.1080/16878507.2019.1702243
Mirković MD, Milanović Z, Stanković DM, Petrović Đ, Vranješ-Đurić S, Janković D, Radović M. Investigation of 177Lu-labeled HEDP, DPD, and IDP as potential bone pain palliation agents. in Journal of Radiation Research and Applied Sciences. 2020;13(1):27-36.
doi:10.1080/16878507.2019.1702243 .
Mirković, Marija D., Milanović, Zorana, Stanković, Dalibor M., Petrović, Đorđe, Vranješ-Đurić, Sanja, Janković, Drina, Radović, Magdalena, "Investigation of 177Lu-labeled HEDP, DPD, and IDP as potential bone pain palliation agents" in Journal of Radiation Research and Applied Sciences, 13, no. 1 (2020):27-36,
https://doi.org/10.1080/16878507.2019.1702243 . .
6

Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis

Milanović, Zorana; Beletić, Anđelo; Vekić, Jelena; Zeljković, Aleksandra; Andrić, Nenad; Ilić Božović, Anja; Spariosu, Kristina; Radaković, Milena; Ajtić, Jelena; Kovačević-Filipović, Milica M.

(2020)

TY  - JOUR
AU  - Milanović, Zorana
AU  - Beletić, Anđelo
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Andrić, Nenad
AU  - Ilić Božović, Anja
AU  - Spariosu, Kristina
AU  - Radaković, Milena
AU  - Ajtić, Jelena
AU  - Kovačević-Filipović, Milica M.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9011
AB  - Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.
T2  - Veterinary Parasitology
T1  - Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis
VL  - 282
SP  - 109140
DO  - 10.1016/j.vetpar.2020.109140
ER  - 
@article{
author = "Milanović, Zorana and Beletić, Anđelo and Vekić, Jelena and Zeljković, Aleksandra and Andrić, Nenad and Ilić Božović, Anja and Spariosu, Kristina and Radaković, Milena and Ajtić, Jelena and Kovačević-Filipović, Milica M.",
year = "2020",
abstract = "Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.",
journal = "Veterinary Parasitology",
title = "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis",
volume = "282",
pages = "109140",
doi = "10.1016/j.vetpar.2020.109140"
}
Milanović, Z., Beletić, A., Vekić, J., Zeljković, A., Andrić, N., Ilić Božović, A., Spariosu, K., Radaković, M., Ajtić, J.,& Kovačević-Filipović, M. M.. (2020). Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology, 282, 109140.
https://doi.org/10.1016/j.vetpar.2020.109140
Milanović Z, Beletić A, Vekić J, Zeljković A, Andrić N, Ilić Božović A, Spariosu K, Radaković M, Ajtić J, Kovačević-Filipović MM. Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology. 2020;282:109140.
doi:10.1016/j.vetpar.2020.109140 .
Milanović, Zorana, Beletić, Anđelo, Vekić, Jelena, Zeljković, Aleksandra, Andrić, Nenad, Ilić Božović, Anja, Spariosu, Kristina, Radaković, Milena, Ajtić, Jelena, Kovačević-Filipović, Milica M., "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis" in Veterinary Parasitology, 282 (2020):109140,
https://doi.org/10.1016/j.vetpar.2020.109140 . .
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