TY  - CONF
AU  - Životić, Ivan
AU  - Mitrović, Kristina
AU  - Kolić, Ivana
AU  - Seke, Mariana
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12465
AB  - Introduction: Congenital anomalies of the kidney and urinary tracts(CAKUT) are a diverse spectrum of defects with complex etiology and not fully explained genetic background. miRNA-containing copy number variants (CNVs) are described as genetic risk factor for the disease development. We aimed to identify miRNAs with the maximum regulatory coverage of previously reported differentially expressed genes in CAKUT tissue compared to controls and bioinformatically characterize a set of these miRNAs which are located in common CNVs. Methods: Differentially expressed genes were identified from ureter tissue transcriptome open data GSE83946 from 15 CAKUT patients and 7 healthy controls, generated in house previously. miRPathDB v2.0 was used for identification of miRNAs with maximum coverage of DEGs(miRNAs which complimentarily regulate all DEGs). Mapping of maximum coverage miRNAs onto common CNVs (frequency >0.2) was performed using UCSC genome browser and gnomAD database. miRNA mapping common CNVs were further bioinformatically analyzed using miRPathDB v2.0. Results: In a maximum coverage set of 50 miRNAs interacting with DEGs in CAKUT, we have identified 3 miRNA geneslocated in the common CNVs(hsa-miR-663b, hsa-miR-3180-3p and hsa-miR-1302). Using Reactome database we identified all three miRNAsto be significantly enriched in the pathway Neuronal System: -log(p-value)>2.326 for hsa-miR-1302; -log(p-value)>1.556 for hsa-miR-3180-3p; and -log(pvalue)>1.703 for hsa-miR-663b. Conclusion: CAKUT is characterized with variable penetrability and expressivity and often followed with other comorbiditiessuch as neurodevelopmental disorders. miRNAsinvolved in DEG networks and prone to CNV effects could present modulating factors of the disease phenotype. Further studies should provide additional evidence about hsa-miR-1302, hsa-miR-3180-3p and hsa-miR-663b involvements in CAKUT etiology
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts
T1  - Identification of micro RNA from common copy number variants as risk factors for CAKUT
SP  - 62
EP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12465
ER  - 

@conference{
author = "Životić, Ivan and Mitrović, Kristina and Kolić, Ivana and Seke, Mariana and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan",
year = "2023",
abstract = "Introduction: Congenital anomalies of the kidney and urinary tracts(CAKUT) are a diverse spectrum of defects with complex etiology and not fully explained genetic background. miRNA-containing copy number variants (CNVs) are described as genetic risk factor for the disease development. We aimed to identify miRNAs with the maximum regulatory coverage of previously reported differentially expressed genes in CAKUT tissue compared to controls and bioinformatically characterize a set of these miRNAs which are located in common CNVs. Methods: Differentially expressed genes were identified from ureter tissue transcriptome open data GSE83946 from 15 CAKUT patients and 7 healthy controls, generated in house previously. miRPathDB v2.0 was used for identification of miRNAs with maximum coverage of DEGs(miRNAs which complimentarily regulate all DEGs). Mapping of maximum coverage miRNAs onto common CNVs (frequency >0.2) was performed using UCSC genome browser and gnomAD database. miRNA mapping common CNVs were further bioinformatically analyzed using miRPathDB v2.0. Results: In a maximum coverage set of 50 miRNAs interacting with DEGs in CAKUT, we have identified 3 miRNA geneslocated in the common CNVs(hsa-miR-663b, hsa-miR-3180-3p and hsa-miR-1302). Using Reactome database we identified all three miRNAsto be significantly enriched in the pathway Neuronal System: -log(p-value)>2.326 for hsa-miR-1302; -log(p-value)>1.556 for hsa-miR-3180-3p; and -log(pvalue)>1.703 for hsa-miR-663b. Conclusion: CAKUT is characterized with variable penetrability and expressivity and often followed with other comorbiditiessuch as neurodevelopmental disorders. miRNAsinvolved in DEG networks and prone to CNV effects could present modulating factors of the disease phenotype. Further studies should provide additional evidence about hsa-miR-1302, hsa-miR-3180-3p and hsa-miR-663b involvements in CAKUT etiology",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts",
title = "Identification of micro RNA from common copy number variants as risk factors for CAKUT",
pages = "62-62",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12465"
}

Životić, I., Mitrović, K., Kolić, I., Seke, M., Živković, M., Stanković, A.,& Jovanović, I.. (2023). Identification of micro RNA from common copy number variants as risk factors for CAKUT. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts, 62-62.
https://hdl.handle.net/21.15107/rcub_vinar_12465

Životić I, Mitrović K, Kolić I, Seke M, Živković M, Stanković A, Jovanović I. Identification of micro RNA from common copy number variants as risk factors for CAKUT. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts. 2023;:62-62.
https://hdl.handle.net/21.15107/rcub_vinar_12465 .

Životić, Ivan, Mitrović, Kristina, Kolić, Ivana, Seke, Mariana, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan, "Identification of micro RNA from common copy number variants as risk factors for CAKUT" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts (2023):62-62,
https://hdl.handle.net/21.15107/rcub_vinar_12465 .

TY  - CONF
AU  - Kokunešoski, Maja
AU  - Šaponjić, Aleksandra
AU  - Baščarević, Zvezdana
AU  - Jovanović, Ivan
AU  - Filipović Tričković, Jelena G.
AU  - Valenta-Šobot, Ana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11280
AB  - Material SBA-15 with all of its particles in the form of regular spheres was synthesised by template method using
Pluronic P123 (non-ionic triblock copolymer) as a surfactant and tetraethoxysilane (TEOS, 98%) as a source of silica.
The synthesis of spherical SBA-15 was carried out in acidic conditions according to method proposed by Zhao et al.
Instead of expensive commercial cetyltrimethylammonium bromide as a co-surfactant, a spent HCl solution obtained
after the chemical treatment of clay in an aqueous solution of HCl was used. The mixture was aged at a higher
temperature. After that, the spherical SBA-15 was made by drying at room temperature after washing with distilled
water and then calcining in flowing air to decompose the triblock copolymer. The continual presence of spheres
of diameters around 2 μm in the entire SBA-15 was confirmed by scanning electron microscopy. The Energy-dispersive
X-ray spectroscopy confirmed that the spheres consisted only of SiO2 in composition. In addition, X-ray diffraction and
Fourier-transform infrared spectroscopy methods were used to characterise SBA-15 material. To assess the cytotoxic
effects of newly synthesised SBA-15 material, HEK 293 cells were treated with particles and extracts obtained from
particles incubated 24 hours at 37 °C in serum-free DMEM medium and DMEM with 10% serum. Cells were treated
with concentrations of 100, 250, and 500 µg/mL for 72 hours. All treatments reduced cell viability in a concentrationdependent manner (p<0.001). Cytotoxicity was more pronounced in particle treatment, indicating that mechanical
damage was induced in cells, whereas the extraction process influences the level of toxicity since the sera-free medium
had the mildest effect. To acquire safe particles for human use, additional synthesis optimisation is required.
PB  - Association of Metallurgical Engineers of Serbia (AMES)
C3  - MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina
T1  - Preliminary cytotoxicity testing of newly synthesised SBA-15 material
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11280
ER  - 

@conference{
author = "Kokunešoski, Maja and Šaponjić, Aleksandra and Baščarević, Zvezdana and Jovanović, Ivan and Filipović Tričković, Jelena G. and Valenta-Šobot, Ana",
year = "2023",
abstract = "Material SBA-15 with all of its particles in the form of regular spheres was synthesised by template method using
Pluronic P123 (non-ionic triblock copolymer) as a surfactant and tetraethoxysilane (TEOS, 98%) as a source of silica.
The synthesis of spherical SBA-15 was carried out in acidic conditions according to method proposed by Zhao et al.
Instead of expensive commercial cetyltrimethylammonium bromide as a co-surfactant, a spent HCl solution obtained
after the chemical treatment of clay in an aqueous solution of HCl was used. The mixture was aged at a higher
temperature. After that, the spherical SBA-15 was made by drying at room temperature after washing with distilled
water and then calcining in flowing air to decompose the triblock copolymer. The continual presence of spheres
of diameters around 2 μm in the entire SBA-15 was confirmed by scanning electron microscopy. The Energy-dispersive
X-ray spectroscopy confirmed that the spheres consisted only of SiO2 in composition. In addition, X-ray diffraction and
Fourier-transform infrared spectroscopy methods were used to characterise SBA-15 material. To assess the cytotoxic
effects of newly synthesised SBA-15 material, HEK 293 cells were treated with particles and extracts obtained from
particles incubated 24 hours at 37 °C in serum-free DMEM medium and DMEM with 10% serum. Cells were treated
with concentrations of 100, 250, and 500 µg/mL for 72 hours. All treatments reduced cell viability in a concentrationdependent manner (p<0.001). Cytotoxicity was more pronounced in particle treatment, indicating that mechanical
damage was induced in cells, whereas the extraction process influences the level of toxicity since the sera-free medium
had the mildest effect. To acquire safe particles for human use, additional synthesis optimisation is required.",
publisher = "Association of Metallurgical Engineers of Serbia (AMES)",
journal = "MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina",
title = "Preliminary cytotoxicity testing of newly synthesised SBA-15 material",
pages = "60",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11280"
}

Kokunešoski, M., Šaponjić, A., Baščarević, Z., Jovanović, I., Filipović Tričković, J. G.,& Valenta-Šobot, A.. (2023). Preliminary cytotoxicity testing of newly synthesised SBA-15 material. in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina
Association of Metallurgical Engineers of Serbia (AMES)., 60.
https://hdl.handle.net/21.15107/rcub_vinar_11280

Kokunešoski M, Šaponjić A, Baščarević Z, Jovanović I, Filipović Tričković JG, Valenta-Šobot A. Preliminary cytotoxicity testing of newly synthesised SBA-15 material. in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina. 2023;:60.
https://hdl.handle.net/21.15107/rcub_vinar_11280 .

Kokunešoski, Maja, Šaponjić, Aleksandra, Baščarević, Zvezdana, Jovanović, Ivan, Filipović Tričković, Jelena G., Valenta-Šobot, Ana, "Preliminary cytotoxicity testing of newly synthesised SBA-15 material" in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina (2023):60,
https://hdl.handle.net/21.15107/rcub_vinar_11280 .

TY  - CONF
AU  - Kolić, Ivana
AU  - Mitrović, Kristina
AU  - Životić, Ivan
AU  - Đorđević, Ana
AU  - Filipović-Tričković, Jelena
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12684
AB  - Background/Objectives: Rare copy number variants (rCNVs) are the common genetic cause of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT).1 miRNAs located in rCNVs represent well-founded functional variants for human CAKUT research. However, the impact of rCNVs on miRNA genes in CAKUT is unknown. Thus, burden assessment was performed to identify chromosomes with non-random representation of miRNA genes in rCNVs associated with CAKUT. Methods: A comprehensive literature mining of rCNV regions associated with CAKUT was performed. The total cumulative length of rCNVs per chromosome was the sum of corresponding CNV-DNA regions, taking into account overlapping. Mapping of miRNAs onto cumulative rCNV regions gave counts of affected miRNA loci. The correlation analysis was performed between the number of miRNA genes overlapping rCNVs, and the fractional lengths of cumulative rCNVs regions in relation to the chromosome size. Results: A statistically significant positive correlation was observed for duplications and deletions respectively (Spearman correlation p<0.0001, r=0.9, r=0.8). However, a deviation from the best fit line for chromosome 16, for both rare duplications and deletions, was observed due to the high overrepresentation of miRNA genes in identified rCNVs. Conclusion: The current finding of the high overall burden of rCNVs on miRNA genes in chromosome 16 suggests that miRNAs located on this chromosome could serve as candidates for the investigation of miRNA role in CAKUT development.
C3  - 54th European Society of Human Genetics (ESHG) : Book of abstracts
T1  - Assessing the burden of rare CNVs on miRNA genes in CAKUT
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12684
ER  - 

@conference{
author = "Kolić, Ivana and Mitrović, Kristina and Životić, Ivan and Đorđević, Ana and Filipović-Tričković, Jelena and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan",
year = "2022",
abstract = "Background/Objectives: Rare copy number variants (rCNVs) are the common genetic cause of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT).1 miRNAs located in rCNVs represent well-founded functional variants for human CAKUT research. However, the impact of rCNVs on miRNA genes in CAKUT is unknown. Thus, burden assessment was performed to identify chromosomes with non-random representation of miRNA genes in rCNVs associated with CAKUT. Methods: A comprehensive literature mining of rCNV regions associated with CAKUT was performed. The total cumulative length of rCNVs per chromosome was the sum of corresponding CNV-DNA regions, taking into account overlapping. Mapping of miRNAs onto cumulative rCNV regions gave counts of affected miRNA loci. The correlation analysis was performed between the number of miRNA genes overlapping rCNVs, and the fractional lengths of cumulative rCNVs regions in relation to the chromosome size. Results: A statistically significant positive correlation was observed for duplications and deletions respectively (Spearman correlation p<0.0001, r=0.9, r=0.8). However, a deviation from the best fit line for chromosome 16, for both rare duplications and deletions, was observed due to the high overrepresentation of miRNA genes in identified rCNVs. Conclusion: The current finding of the high overall burden of rCNVs on miRNA genes in chromosome 16 suggests that miRNAs located on this chromosome could serve as candidates for the investigation of miRNA role in CAKUT development.",
journal = "54th European Society of Human Genetics (ESHG) : Book of abstracts",
title = "Assessing the burden of rare CNVs on miRNA genes in CAKUT",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12684"
}

Kolić, I., Mitrović, K., Životić, I., Đorđević, A., Filipović-Tričković, J., Živković, M., Stanković, A.,& Jovanović, I.. (2022). Assessing the burden of rare CNVs on miRNA genes in CAKUT. in 54th European Society of Human Genetics (ESHG) : Book of abstracts.
https://hdl.handle.net/21.15107/rcub_vinar_12684

Kolić I, Mitrović K, Životić I, Đorđević A, Filipović-Tričković J, Živković M, Stanković A, Jovanović I. Assessing the burden of rare CNVs on miRNA genes in CAKUT. in 54th European Society of Human Genetics (ESHG) : Book of abstracts. 2022;.
https://hdl.handle.net/21.15107/rcub_vinar_12684 .

Kolić, Ivana, Mitrović, Kristina, Životić, Ivan, Đorđević, Ana, Filipović-Tričković, Jelena, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan, "Assessing the burden of rare CNVs on miRNA genes in CAKUT" in 54th European Society of Human Genetics (ESHG) : Book of abstracts (2022),
https://hdl.handle.net/21.15107/rcub_vinar_12684 .

TY  - CONF
AU  - Životić, Ivan
AU  - Jovanović, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Dekleva, Milica
AU  - Marković Nikolić, Nevena
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12816
AB  - Introduction: Recently, rs2275888 eQTL in PTPLAD2 gene has been associated with expression of several loci, during inflammatory stimulation in monocytes. Myocardial infarction (MI) triggers an intense inflammatory response that is essential for cardiac repair. We aimed to perform data scouting in appropriate rs2275888 genotype model to identify differentially expressed genes (DEGs), their biological meaning, and key miRs potentially associated with rs2275888 eQTL in peripheral blood mononuclear leucocytes (PBML) of MI patients 6 months after first MI. Methods: Transcriptome data was obtained from PBMLs of 21 patients, who suffered ischemic MI, by employing Illumina iScan microarray technology. Genotyping for rs2275888 was conducted with real-time PCR, using TaqMan® assay. Preprocessing and identification of DEGs was done using limma package of R/Bioconductor software. The online tool DAVID v6.8 was employed for functional enrichment analysis. Most important miRs were selected using NetworkAnalyst web tool, based on the degree centrality value. Results: Transcriptome analysis in recessive model TT+TC (n=19) vs. CC (n=2) identified 68 DEGs. Top significant biological processes involving DEGs cover vascular physiology, cell growth and signaling. Pathway analysis associated DEGs with adherens junction, Rap1 and Ras signaling. Network analysis identified hsa-miR335-5p, -26b-5p, -93-5p, -16-5p, -124-3p, -20b-5p, -17-5p and -218-5p as miRs with top centrality degree in our DEGs list. Conclusion: Seven of eight detected miRs have already been described in MI pathology or suggested as potential biomarkers for MI. Our result suggest the importance of integration of eQTLs, biological processes and pathway analysis coupled with miR activity for further research in MI pathology.
PB  - Belgrade : University of Belgrade, Faculty of Biology
C3  - CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
T1  - Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach
SP  - 176
EP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12816
ER  - 

@conference{
author = "Životić, Ivan and Jovanović, Ivan and Đurić, Tamara and Stanković, Aleksandra and Dekleva, Milica and Marković Nikolić, Nevena and Alavantić, Dragan and Živković, Maja",
year = "2017",
abstract = "Introduction: Recently, rs2275888 eQTL in PTPLAD2 gene has been associated with expression of several loci, during inflammatory stimulation in monocytes. Myocardial infarction (MI) triggers an intense inflammatory response that is essential for cardiac repair. We aimed to perform data scouting in appropriate rs2275888 genotype model to identify differentially expressed genes (DEGs), their biological meaning, and key miRs potentially associated with rs2275888 eQTL in peripheral blood mononuclear leucocytes (PBML) of MI patients 6 months after first MI. Methods: Transcriptome data was obtained from PBMLs of 21 patients, who suffered ischemic MI, by employing Illumina iScan microarray technology. Genotyping for rs2275888 was conducted with real-time PCR, using TaqMan® assay. Preprocessing and identification of DEGs was done using limma package of R/Bioconductor software. The online tool DAVID v6.8 was employed for functional enrichment analysis. Most important miRs were selected using NetworkAnalyst web tool, based on the degree centrality value. Results: Transcriptome analysis in recessive model TT+TC (n=19) vs. CC (n=2) identified 68 DEGs. Top significant biological processes involving DEGs cover vascular physiology, cell growth and signaling. Pathway analysis associated DEGs with adherens junction, Rap1 and Ras signaling. Network analysis identified hsa-miR335-5p, -26b-5p, -93-5p, -16-5p, -124-3p, -20b-5p, -17-5p and -218-5p as miRs with top centrality degree in our DEGs list. Conclusion: Seven of eight detected miRs have already been described in MI pathology or suggested as potential biomarkers for MI. Our result suggest the importance of integration of eQTLs, biological processes and pathway analysis coupled with miR activity for further research in MI pathology.",
publisher = "Belgrade : University of Belgrade, Faculty of Biology",
journal = "CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts",
title = "Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach",
pages = "176-176",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12816"
}

Životić, I., Jovanović, I., Đurić, T., Stanković, A., Dekleva, M., Marković Nikolić, N., Alavantić, D.,& Živković, M.. (2017). Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
Belgrade : University of Belgrade, Faculty of Biology., 176-176.
https://hdl.handle.net/21.15107/rcub_vinar_12816

Životić I, Jovanović I, Đurić T, Stanković A, Dekleva M, Marković Nikolić N, Alavantić D, Živković M. Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts. 2017;:176-176.
https://hdl.handle.net/21.15107/rcub_vinar_12816 .

Životić, Ivan, Jovanović, Ivan, Đurić, Tamara, Stanković, Aleksandra, Dekleva, Milica, Marković Nikolić, Nevena, Alavantić, Dragan, Živković, Maja, "Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach" in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts (2017):176-176,
https://hdl.handle.net/21.15107/rcub_vinar_12816 .

TY  - JOUR
AU  - Šuluburic, Adam
AU  - Milanovic, Svetlana
AU  - Vranješ-Đurić, Sanja
AU  - Jovanović, Ivan B.
AU  - Barna, Tomislav
AU  - Stojić, Milica
AU  - Fratric, Natalija
AU  - Szenci, Otto
AU  - Gvozdic, Dragan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1757
AB  - Early embryonic development may be negatively affected by insufficient progesterone (P4) production. Therefore, the aim of our study was to increase P4 by gonadotropin-releasing hormone (GnRH) and/or human chorionic gonadotropin (hCG) treatments after inducing oestrus by prostaglandin (PG) treatment. Lactating Simmental dairy cows (n = 110), between 1 to 5 lactations, with an average milk production of 6,500 l/305 days, at 40-80 days postpartum were used and grouped as follows: (1) PG + GnRH treatment at AI (GnRH group), (2) PG + hCG treatment at day 7 after AI (hCG group), (3) PG + GnRH at AI + hCG treatment at day 7 after AI (GnRH/hCG group), and (4) spontaneous oestrus (C: control group). All animals were double inseminated (at the time of oestrus detection and 12 +/- 2 h thereafter). Blood serum and milk samples were collected at the day of observed oestrus (day 0), and 14, 21 and 28 days after AI. Serum P4 was determined using a commercial radioimmunoassay (RIA) test (INEP, Zemun), and milk P4 was determined using enzyme-linked immunoassay (ELISA) test (NIV Novi Sad). Pregnancy status was confirmed by ultrasonography between days 28 and 35 after AI. Differences of serum or milk P4 medians, pregnancy (and calving) rate were determined using Dunns Multiple Comparison Tests and Z test, respectively. Serum P4 medians were significantly higher at days 14, 21 and 28 after AI in the hCG-treated animals, indicating increased luteal activity, with a similar tendency in whole milk P4 values. Treatment with hCG during the early luteal phase significantly contributed to the maintenance of gestation at days 28-35 after AI, and also increased the calving rate in Simmental dairy cows.
T2  - Acta Veterinaria Hungarica
T1  - Progesterone Concentration, Pregnancy and Calving Rate in Simmental Dairy Cows After Oestrus Synchronisation and Hcg Treatment During the Early Luteal Phase
VL  - 65
IS  - 3
SP  - 446
EP  - 458
DO  - 10.1556/004.2017.042
ER  - 

@article{
author = "Šuluburic, Adam and Milanovic, Svetlana and Vranješ-Đurić, Sanja and Jovanović, Ivan B. and Barna, Tomislav and Stojić, Milica and Fratric, Natalija and Szenci, Otto and Gvozdic, Dragan",
year = "2017",
abstract = "Early embryonic development may be negatively affected by insufficient progesterone (P4) production. Therefore, the aim of our study was to increase P4 by gonadotropin-releasing hormone (GnRH) and/or human chorionic gonadotropin (hCG) treatments after inducing oestrus by prostaglandin (PG) treatment. Lactating Simmental dairy cows (n = 110), between 1 to 5 lactations, with an average milk production of 6,500 l/305 days, at 40-80 days postpartum were used and grouped as follows: (1) PG + GnRH treatment at AI (GnRH group), (2) PG + hCG treatment at day 7 after AI (hCG group), (3) PG + GnRH at AI + hCG treatment at day 7 after AI (GnRH/hCG group), and (4) spontaneous oestrus (C: control group). All animals were double inseminated (at the time of oestrus detection and 12 +/- 2 h thereafter). Blood serum and milk samples were collected at the day of observed oestrus (day 0), and 14, 21 and 28 days after AI. Serum P4 was determined using a commercial radioimmunoassay (RIA) test (INEP, Zemun), and milk P4 was determined using enzyme-linked immunoassay (ELISA) test (NIV Novi Sad). Pregnancy status was confirmed by ultrasonography between days 28 and 35 after AI. Differences of serum or milk P4 medians, pregnancy (and calving) rate were determined using Dunns Multiple Comparison Tests and Z test, respectively. Serum P4 medians were significantly higher at days 14, 21 and 28 after AI in the hCG-treated animals, indicating increased luteal activity, with a similar tendency in whole milk P4 values. Treatment with hCG during the early luteal phase significantly contributed to the maintenance of gestation at days 28-35 after AI, and also increased the calving rate in Simmental dairy cows.",
journal = "Acta Veterinaria Hungarica",
title = "Progesterone Concentration, Pregnancy and Calving Rate in Simmental Dairy Cows After Oestrus Synchronisation and Hcg Treatment During the Early Luteal Phase",
volume = "65",
number = "3",
pages = "446-458",
doi = "10.1556/004.2017.042"
}

Šuluburic, A., Milanovic, S., Vranješ-Đurić, S., Jovanović, I. B., Barna, T., Stojić, M., Fratric, N., Szenci, O.,& Gvozdic, D.. (2017). Progesterone Concentration, Pregnancy and Calving Rate in Simmental Dairy Cows After Oestrus Synchronisation and Hcg Treatment During the Early Luteal Phase. in Acta Veterinaria Hungarica, 65(3), 446-458.
https://doi.org/10.1556/004.2017.042

Šuluburic A, Milanovic S, Vranješ-Đurić S, Jovanović IB, Barna T, Stojić M, Fratric N, Szenci O, Gvozdic D. Progesterone Concentration, Pregnancy and Calving Rate in Simmental Dairy Cows After Oestrus Synchronisation and Hcg Treatment During the Early Luteal Phase. in Acta Veterinaria Hungarica. 2017;65(3):446-458.
doi:10.1556/004.2017.042 .

Šuluburic, Adam, Milanovic, Svetlana, Vranješ-Đurić, Sanja, Jovanović, Ivan B., Barna, Tomislav, Stojić, Milica, Fratric, Natalija, Szenci, Otto, Gvozdic, Dragan, "Progesterone Concentration, Pregnancy and Calving Rate in Simmental Dairy Cows After Oestrus Synchronisation and Hcg Treatment During the Early Luteal Phase" in Acta Veterinaria Hungarica, 65, no. 3 (2017):446-458,
https://doi.org/10.1556/004.2017.042 . .

TY  - JOUR
AU  - Jovanović, Ivan B.
AU  - Velickovic, Miljan
AU  - Milanovic, Svetlana
AU  - Valcic, Olivera
AU  - Gvozdic, Dragan
AU  - Vranješ-Đurić, Sanja
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/626
AB  - The aim of this investigation was to determine the influence of oxidative stress upon general stress in dairy cows on parturition and whether the indicators of stress can be reduced by selenium (Se) supplementation. A total of 36 animals were divided into 3 groups 21 days prepartum and treated with a single-term intra muscular supplement of sodium selenite: Control group - 0 mg; group Se10 - 10 mg; group Se20 - 20 mg. Se supplementation significantly raised blood Se content and glutathione peroxidase (GPx) activity in groups Se10 and Se20, compared to Control, although there was no marked difference between supplemented groups. Plasma malondialdehyde (MDA) and cortisol concentrations were significantly reduced in supplemented groups Se10 and Se20, compared to Control. A negative correlation was detected between blood GPx activity and plasma MDA, while a positive correlation was determined between plasma MDA and cortisol concentrations. These results indicate that prepartum Se supplementation can be utilized for a partial relief of stress in cows during labor by augmenting the antioxidative action of GPx.
T2  - Acta Veterinaria, Beograd
T1  - Supplemental Selenium Reduces the Levels of Biomarkers of Oxidative and General Stress in Peripartum Dairy Cows
VL  - 65
IS  - 2
SP  - 191
EP  - 201
DO  - 10.1515/acve-2015-0016
ER  - 

@article{
author = "Jovanović, Ivan B. and Velickovic, Miljan and Milanovic, Svetlana and Valcic, Olivera and Gvozdic, Dragan and Vranješ-Đurić, Sanja",
year = "2015",
abstract = "The aim of this investigation was to determine the influence of oxidative stress upon general stress in dairy cows on parturition and whether the indicators of stress can be reduced by selenium (Se) supplementation. A total of 36 animals were divided into 3 groups 21 days prepartum and treated with a single-term intra muscular supplement of sodium selenite: Control group - 0 mg; group Se10 - 10 mg; group Se20 - 20 mg. Se supplementation significantly raised blood Se content and glutathione peroxidase (GPx) activity in groups Se10 and Se20, compared to Control, although there was no marked difference between supplemented groups. Plasma malondialdehyde (MDA) and cortisol concentrations were significantly reduced in supplemented groups Se10 and Se20, compared to Control. A negative correlation was detected between blood GPx activity and plasma MDA, while a positive correlation was determined between plasma MDA and cortisol concentrations. These results indicate that prepartum Se supplementation can be utilized for a partial relief of stress in cows during labor by augmenting the antioxidative action of GPx.",
journal = "Acta Veterinaria, Beograd",
title = "Supplemental Selenium Reduces the Levels of Biomarkers of Oxidative and General Stress in Peripartum Dairy Cows",
volume = "65",
number = "2",
pages = "191-201",
doi = "10.1515/acve-2015-0016"
}

Jovanović, I. B., Velickovic, M., Milanovic, S., Valcic, O., Gvozdic, D.,& Vranješ-Đurić, S.. (2015). Supplemental Selenium Reduces the Levels of Biomarkers of Oxidative and General Stress in Peripartum Dairy Cows. in Acta Veterinaria, Beograd, 65(2), 191-201.
https://doi.org/10.1515/acve-2015-0016

Jovanović IB, Velickovic M, Milanovic S, Valcic O, Gvozdic D, Vranješ-Đurić S. Supplemental Selenium Reduces the Levels of Biomarkers of Oxidative and General Stress in Peripartum Dairy Cows. in Acta Veterinaria, Beograd. 2015;65(2):191-201.
doi:10.1515/acve-2015-0016 .

Jovanović, Ivan B., Velickovic, Miljan, Milanovic, Svetlana, Valcic, Olivera, Gvozdic, Dragan, Vranješ-Đurić, Sanja, "Supplemental Selenium Reduces the Levels of Biomarkers of Oxidative and General Stress in Peripartum Dairy Cows" in Acta Veterinaria, Beograd, 65, no. 2 (2015):191-201,
https://doi.org/10.1515/acve-2015-0016 . .