Petrović, Nina

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Authority KeyName Variants
orcid::0000-0003-2503-1228
  • Petrović, Nina (29)
Projects
Molecular determinants for tumor marker design Pain Control and Molecular Mechanisms as Factors for Tissue Regeneration in Dentistry in Healthy and Diabetic Patients
Hormonal regulation of expression and activity of the nitric oxide synthase and sodium-potassium pump in experimental models of insulin resistance, diabetes and cardiovascular disorders Biological response modifiers in physiological and pathological conditions
Synthesis, modeling, physicochemical and biological properties of organic compounds and related metal complexes Molecular mechanisms of cellular responses on pathological changes in central neuronal system and peripheral organs of mammals
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200043 (Institute of Oncology and Radiology of Serbia, Belgrade) Mechanistic studies of the reactions of transition metal ion complexes with biologically relevant molecules
Interactions of natural products, their derivatives and coordination compounds with proteins and nucleic acids Phylogenetic anaysis and molecular evolution of highly variable viruses: coinfections, host-pathogene interactions
Genetic basis of human vascular and inflammatory diseases Effects of modulation of biohumoral, inflammatory and metabolic response in acute ST-segment elevation myocardial infarction on survival and left ventricular function
The interaction of xenobiotics with biological systems Nagasaki University Global COE Program
Ordu University, Scientific Research Projects Coordination Unit Ordu University, Scientific Research Projects Coordination Unit, Turkey [AR-1609]
Scientific Research Projects Unit of Ordu University [A-1823] Serbian Ministry of Sciences Technological Development [141041]

Author's Bibliography

Breast Cancer Response to Therapy: Can microRNAs Lead the Way?

Petrović, Nina; Nakashidze, Irina; Nedeljković, Milica

(2021)

TY  - JOUR
AU  - Petrović, Nina
AU  - Nakashidze, Irina
AU  - Nedeljković, Milica
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9555
AB  - Breast cancer (BC) is a leading cause of death among women with malignant diseases. The selection of adequate therapies for highly invasive and metastatic BCs still represents a major challenge. Novel combinatorial therapeutic approaches are urgently required to enhance the efficiency of BC treatment. Recently, microRNAs (miRNAs) emerged as key regulators of the complex mechanisms that govern BC therapeutic resistance and susceptibility. In the present review we aim to critically examine how miRNAs influence BC response to therapies, or how to use miRNAs as a basis for new therapeutic approaches. We summarized recent findings in this rapidly evolving field, emphasizing the challenges still ahead for the successful implementation of miRNAs into BC treatment while providing insights for future BC management. The goal of this review was to propose miRNAs, that might simultaneously improve the efficacy of all four therapies that are the backbone of current BC management (radio-, chemo-, targeted, and hormone therapy). Among the fifty-nine described miRNAs, miR-21 and miR-16 emerged as the most promising, closely followed by miR-205, miR-451, miR-182, and miRNAs from the let-7 family. miR-21 inhibition might be the best choice for future improvement of invasive BC treatment. New therapeutic strategies of miRNA-based agents alongside current standard treatment modalities could greatly benefit BC patients. This review represents a guideline on how to navigate this elaborate puzzle. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.
T2  - Journal of Mammary Gland Biology and Neoplasia
T1  - Breast Cancer Response to Therapy: Can microRNAs Lead the Way?
VL  - 26
IS  - 2
SP  - 157
EP  - 178
DO  - 10.1007/s10911-021-09478-3
ER  - 
@article{
author = "Petrović, Nina and Nakashidze, Irina and Nedeljković, Milica",
year = "2021",
abstract = "Breast cancer (BC) is a leading cause of death among women with malignant diseases. The selection of adequate therapies for highly invasive and metastatic BCs still represents a major challenge. Novel combinatorial therapeutic approaches are urgently required to enhance the efficiency of BC treatment. Recently, microRNAs (miRNAs) emerged as key regulators of the complex mechanisms that govern BC therapeutic resistance and susceptibility. In the present review we aim to critically examine how miRNAs influence BC response to therapies, or how to use miRNAs as a basis for new therapeutic approaches. We summarized recent findings in this rapidly evolving field, emphasizing the challenges still ahead for the successful implementation of miRNAs into BC treatment while providing insights for future BC management. The goal of this review was to propose miRNAs, that might simultaneously improve the efficacy of all four therapies that are the backbone of current BC management (radio-, chemo-, targeted, and hormone therapy). Among the fifty-nine described miRNAs, miR-21 and miR-16 emerged as the most promising, closely followed by miR-205, miR-451, miR-182, and miRNAs from the let-7 family. miR-21 inhibition might be the best choice for future improvement of invasive BC treatment. New therapeutic strategies of miRNA-based agents alongside current standard treatment modalities could greatly benefit BC patients. This review represents a guideline on how to navigate this elaborate puzzle. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.",
journal = "Journal of Mammary Gland Biology and Neoplasia",
title = "Breast Cancer Response to Therapy: Can microRNAs Lead the Way?",
volume = "26",
number = "2",
pages = "157-178",
doi = "10.1007/s10911-021-09478-3"
}
Petrović, N., Nakashidze, I.,& Nedeljković, M.. (2021). Breast Cancer Response to Therapy: Can microRNAs Lead the Way?. in Journal of Mammary Gland Biology and Neoplasia, 26(2), 157-178.
https://doi.org/10.1007/s10911-021-09478-3
Petrović N, Nakashidze I, Nedeljković M. Breast Cancer Response to Therapy: Can microRNAs Lead the Way?. in Journal of Mammary Gland Biology and Neoplasia. 2021;26(2):157-178.
doi:10.1007/s10911-021-09478-3 .
Petrović, Nina, Nakashidze, Irina, Nedeljković, Milica, "Breast Cancer Response to Therapy: Can microRNAs Lead the Way?" in Journal of Mammary Gland Biology and Neoplasia, 26, no. 2 (2021):157-178,
https://doi.org/10.1007/s10911-021-09478-3 . .
2
2
2
2

Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models

Matić, Ivana Z.; Ergün, Sercan; Đorđić Crnogorac, Marija; Misir, Sema; Aliyazicioğlu, Yüksel; Damjanović, Ana; Džudžević-Čančar, Hurija; Stanojković, Tatjana; Konanç, Kalbiye; Petrović, Nina

(2021)

TY  - JOUR
AU  - Matić, Ivana Z.
AU  - Ergün, Sercan
AU  - Đorđić Crnogorac, Marija
AU  - Misir, Sema
AU  - Aliyazicioğlu, Yüksel
AU  - Damjanović, Ana
AU  - Džudžević-Čančar, Hurija
AU  - Stanojković, Tatjana
AU  - Konanç, Kalbiye
AU  - Petrović, Nina
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9406
AB  - Six extracts were obtained from plant species Hypericum perforatum L., collected at Samsun in Turkey. The aim of this study was to examine the mechanisms of the anticancer activity of these extracts. Methanol, ethyl-acetate and hexane were used as a solvents for extraction from both branch-body part of the plant (extracts 1, 2 and 3) and from plant flowers (extracts 4, 5 and 6). The cytotoxic effects of the extracts were determined against 2D and 3D cancer cell models. Cell cycle changes of treated HeLa cells were analyzed by flow cytometry. Measurements of gene and microRNA expression levels in treated HeLa cells were done by quantitative real time PCR. Five examined extracts (2–6) exerted selective concentration-dependent cytotoxic effects on HeLa, K562, and A549 cancer cells, while the extract 1 exhibited very weak cytotoxicity. The extract 6 showed the highest intensity of cytotoxic activity. All tested extracts (2–6) demonstrated the ability to induce apoptosis in HeLa cells through activation of caspase-3. These extracts remarkably decreased gene expression levels of MMP2, MMP9, TIMP3, and VEGFA in HeLa cells. Flower extracts might have stronger effects on miR128/193a-5p/335 level changes than branch-body extracts. Hypericum perforatum extracts exerted weaker cytotoxic effects on 3D HeLa spheroids when compared with their effects on 2D monolayer HeLa cells. Taken together, results of our research may suggest the promising anticancer properties of the Hypericum perforatum extracts. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
T2  - Cytotechnology
T1  - Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models
VL  - 73
IS  - 3
SP  - 373
EP  - 389
DO  - 10.1007/s10616-021-00464-5
ER  - 
@article{
author = "Matić, Ivana Z. and Ergün, Sercan and Đorđić Crnogorac, Marija and Misir, Sema and Aliyazicioğlu, Yüksel and Damjanović, Ana and Džudžević-Čančar, Hurija and Stanojković, Tatjana and Konanç, Kalbiye and Petrović, Nina",
year = "2021",
abstract = "Six extracts were obtained from plant species Hypericum perforatum L., collected at Samsun in Turkey. The aim of this study was to examine the mechanisms of the anticancer activity of these extracts. Methanol, ethyl-acetate and hexane were used as a solvents for extraction from both branch-body part of the plant (extracts 1, 2 and 3) and from plant flowers (extracts 4, 5 and 6). The cytotoxic effects of the extracts were determined against 2D and 3D cancer cell models. Cell cycle changes of treated HeLa cells were analyzed by flow cytometry. Measurements of gene and microRNA expression levels in treated HeLa cells were done by quantitative real time PCR. Five examined extracts (2–6) exerted selective concentration-dependent cytotoxic effects on HeLa, K562, and A549 cancer cells, while the extract 1 exhibited very weak cytotoxicity. The extract 6 showed the highest intensity of cytotoxic activity. All tested extracts (2–6) demonstrated the ability to induce apoptosis in HeLa cells through activation of caspase-3. These extracts remarkably decreased gene expression levels of MMP2, MMP9, TIMP3, and VEGFA in HeLa cells. Flower extracts might have stronger effects on miR128/193a-5p/335 level changes than branch-body extracts. Hypericum perforatum extracts exerted weaker cytotoxic effects on 3D HeLa spheroids when compared with their effects on 2D monolayer HeLa cells. Taken together, results of our research may suggest the promising anticancer properties of the Hypericum perforatum extracts. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.",
journal = "Cytotechnology",
title = "Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models",
volume = "73",
number = "3",
pages = "373-389",
doi = "10.1007/s10616-021-00464-5"
}
Matić, I. Z., Ergün, S., Đorđić Crnogorac, M., Misir, S., Aliyazicioğlu, Y., Damjanović, A., Džudžević-Čančar, H., Stanojković, T., Konanç, K.,& Petrović, N.. (2021). Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models. in Cytotechnology, 73(3), 373-389.
https://doi.org/10.1007/s10616-021-00464-5
Matić IZ, Ergün S, Đorđić Crnogorac M, Misir S, Aliyazicioğlu Y, Damjanović A, Džudžević-Čančar H, Stanojković T, Konanç K, Petrović N. Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models. in Cytotechnology. 2021;73(3):373-389.
doi:10.1007/s10616-021-00464-5 .
Matić, Ivana Z., Ergün, Sercan, Đorđić Crnogorac, Marija, Misir, Sema, Aliyazicioğlu, Yüksel, Damjanović, Ana, Džudžević-Čančar, Hurija, Stanojković, Tatjana, Konanç, Kalbiye, Petrović, Nina, "Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models" in Cytotechnology, 73, no. 3 (2021):373-389,
https://doi.org/10.1007/s10616-021-00464-5 . .

Comparison of efficacy and safety of preemptive infusion protocols of ephedrine and phenylephrine - prevention of hypotension and effects on hemodynamic parameters during spinal anesthesia for caesarean section

Vukotić, Aleksandra; Green, David; Jevđić, Jasna; Vukotić, Milovan; Petrović, Nina; Stevanović, Predrag

(2020)

TY  - JOUR
AU  - Vukotić, Aleksandra
AU  - Green, David
AU  - Jevđić, Jasna
AU  - Vukotić, Milovan
AU  - Petrović, Nina
AU  - Stevanović, Predrag
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8954
AB  - Introduction/Objective. Spinal anesthesia (SA) for cesarean section may lead to significant changes in hemodynamic parameters, especially hypotension. The aim of this study was to determine and compare the efficacy and safety of preemptive infusion protocols of the two most commonly used vasopressors, ephedrine (Group E, n = 29) and phenylephrine (Group P, n = 31) not only on prevention of hypotension but also to determine their effect on hemodynamic parameters, such as stroke volume (SV) and cardiac output (CO) using a continuous non-invasive hemodynamic monitor. Methods. The infusion of ephedrine was administered at the rate of 5 mg/min. immediately after SA. Phenylephrine was administered at an infusion rate of 25 ?g/min for two minutes prior to SA. Results. In Group E, mean systolic blood pressure (SBP) and heart rate (HR) were similar to baseline. CO was higher (p
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Comparison of efficacy and safety of preemptive infusion protocols of ephedrine and phenylephrine - prevention of hypotension and effects on hemodynamic parameters during spinal anesthesia for caesarean section
VL  - 148
IS  - 3-4
SP  - 173
EP  - 179
DO  - 10.2298/SARH190607009V
ER  - 
@article{
author = "Vukotić, Aleksandra and Green, David and Jevđić, Jasna and Vukotić, Milovan and Petrović, Nina and Stevanović, Predrag",
year = "2020",
abstract = "Introduction/Objective. Spinal anesthesia (SA) for cesarean section may lead to significant changes in hemodynamic parameters, especially hypotension. The aim of this study was to determine and compare the efficacy and safety of preemptive infusion protocols of the two most commonly used vasopressors, ephedrine (Group E, n = 29) and phenylephrine (Group P, n = 31) not only on prevention of hypotension but also to determine their effect on hemodynamic parameters, such as stroke volume (SV) and cardiac output (CO) using a continuous non-invasive hemodynamic monitor. Methods. The infusion of ephedrine was administered at the rate of 5 mg/min. immediately after SA. Phenylephrine was administered at an infusion rate of 25 ?g/min for two minutes prior to SA. Results. In Group E, mean systolic blood pressure (SBP) and heart rate (HR) were similar to baseline. CO was higher (p",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Comparison of efficacy and safety of preemptive infusion protocols of ephedrine and phenylephrine - prevention of hypotension and effects on hemodynamic parameters during spinal anesthesia for caesarean section",
volume = "148",
number = "3-4",
pages = "173-179",
doi = "10.2298/SARH190607009V"
}
Vukotić, A., Green, D., Jevđić, J., Vukotić, M., Petrović, N.,& Stevanović, P.. (2020). Comparison of efficacy and safety of preemptive infusion protocols of ephedrine and phenylephrine - prevention of hypotension and effects on hemodynamic parameters during spinal anesthesia for caesarean section. in Srpski arhiv za celokupno lekarstvo, 148(3-4), 173-179.
https://doi.org/10.2298/SARH190607009V
Vukotić A, Green D, Jevđić J, Vukotić M, Petrović N, Stevanović P. Comparison of efficacy and safety of preemptive infusion protocols of ephedrine and phenylephrine - prevention of hypotension and effects on hemodynamic parameters during spinal anesthesia for caesarean section. in Srpski arhiv za celokupno lekarstvo. 2020;148(3-4):173-179.
doi:10.2298/SARH190607009V .
Vukotić, Aleksandra, Green, David, Jevđić, Jasna, Vukotić, Milovan, Petrović, Nina, Stevanović, Predrag, "Comparison of efficacy and safety of preemptive infusion protocols of ephedrine and phenylephrine - prevention of hypotension and effects on hemodynamic parameters during spinal anesthesia for caesarean section" in Srpski arhiv za celokupno lekarstvo, 148, no. 3-4 (2020):173-179,
https://doi.org/10.2298/SARH190607009V . .
1

Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels

Janković, Nenad Ž.; Trifunović Ristovski, Jovana; Vraneš, Milan; Tot, Aleksandar; Petronijević, Jelena; Joksimović, Nenad; Stanojković, Tatjana P.; Đorđić Crnogorac, Marija; Petrović, Nina; Boljević, Ivana; Matić, Ivana Z.; Bogdanović, Goran A.; Mikov, Momir; Bugarčić, Zorica M.

(2019)

TY  - JOUR
AU  - Janković, Nenad Ž.
AU  - Trifunović Ristovski, Jovana
AU  - Vraneš, Milan
AU  - Tot, Aleksandar
AU  - Petronijević, Jelena
AU  - Joksimović, Nenad
AU  - Stanojković, Tatjana P.
AU  - Đorđić Crnogorac, Marija
AU  - Petrović, Nina
AU  - Boljević, Ivana
AU  - Matić, Ivana Z.
AU  - Bogdanović, Goran A.
AU  - Mikov, Momir
AU  - Bugarčić, Zorica M.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0045206818312598
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8071
AB  - In order to investigate potential therapeutically agents, novel products of Biginelli reaction (4a-l) were synthesized and exposed to cytotoxic and caspase activities, angiogenesis, cell cycle distribution, gene and microRNA expression levels, lipophilicity assessment and docking study. Among the twelve novel compounds (4a-l) evaluated for the cytotoxic activity, five of them (4c, 4d, 4f, 4k and 4l) that showed excellent activity on the tested cell lines (HeLa, LS174 and A549) were selected for further evaluation. Interestingly, compound 4f has up to three times higher selectivity index (SI) towards cancer cells than cisplatin (on HeLa, LS174 and A549 SI = 18.2, 13.5 and 11.2, respectively). The obtained results from cell cycle distribution and caspase activity indicate that tested compounds (4c, 4d, 4f, 4k and 4l) promoted caspase-9 activation, implicated in the intrinsic pathway of apoptosis. Lipophilicity of 4a-l was determinate by using reversed-phase high-performance liquid chromatography. © 2019 Elsevier Inc.
T2  - Bioorganic Chemistry
T1  - Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels
VL  - 86
SP  - 569
EP  - 582
DO  - 10.1016/j.bioorg.2019.02.026
ER  - 
@article{
author = "Janković, Nenad Ž. and Trifunović Ristovski, Jovana and Vraneš, Milan and Tot, Aleksandar and Petronijević, Jelena and Joksimović, Nenad and Stanojković, Tatjana P. and Đorđić Crnogorac, Marija and Petrović, Nina and Boljević, Ivana and Matić, Ivana Z. and Bogdanović, Goran A. and Mikov, Momir and Bugarčić, Zorica M.",
year = "2019",
abstract = "In order to investigate potential therapeutically agents, novel products of Biginelli reaction (4a-l) were synthesized and exposed to cytotoxic and caspase activities, angiogenesis, cell cycle distribution, gene and microRNA expression levels, lipophilicity assessment and docking study. Among the twelve novel compounds (4a-l) evaluated for the cytotoxic activity, five of them (4c, 4d, 4f, 4k and 4l) that showed excellent activity on the tested cell lines (HeLa, LS174 and A549) were selected for further evaluation. Interestingly, compound 4f has up to three times higher selectivity index (SI) towards cancer cells than cisplatin (on HeLa, LS174 and A549 SI = 18.2, 13.5 and 11.2, respectively). The obtained results from cell cycle distribution and caspase activity indicate that tested compounds (4c, 4d, 4f, 4k and 4l) promoted caspase-9 activation, implicated in the intrinsic pathway of apoptosis. Lipophilicity of 4a-l was determinate by using reversed-phase high-performance liquid chromatography. © 2019 Elsevier Inc.",
journal = "Bioorganic Chemistry",
title = "Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels",
volume = "86",
pages = "569-582",
doi = "10.1016/j.bioorg.2019.02.026"
}
Janković, N. Ž., Trifunović Ristovski, J., Vraneš, M., Tot, A., Petronijević, J., Joksimović, N., Stanojković, T. P., Đorđić Crnogorac, M., Petrović, N., Boljević, I., Matić, I. Z., Bogdanović, G. A., Mikov, M.,& Bugarčić, Z. M.. (2019). Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels. in Bioorganic Chemistry, 86, 569-582.
https://doi.org/10.1016/j.bioorg.2019.02.026
Janković NŽ, Trifunović Ristovski J, Vraneš M, Tot A, Petronijević J, Joksimović N, Stanojković TP, Đorđić Crnogorac M, Petrović N, Boljević I, Matić IZ, Bogdanović GA, Mikov M, Bugarčić ZM. Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels. in Bioorganic Chemistry. 2019;86:569-582.
doi:10.1016/j.bioorg.2019.02.026 .
Janković, Nenad Ž., Trifunović Ristovski, Jovana, Vraneš, Milan, Tot, Aleksandar, Petronijević, Jelena, Joksimović, Nenad, Stanojković, Tatjana P., Đorđić Crnogorac, Marija, Petrović, Nina, Boljević, Ivana, Matić, Ivana Z., Bogdanović, Goran A., Mikov, Momir, Bugarčić, Zorica M., "Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels" in Bioorganic Chemistry, 86 (2019):569-582,
https://doi.org/10.1016/j.bioorg.2019.02.026 . .
5
5
5

Significance of miR-15a-5p and CNKSR3 as Novel Prognostic Biomarkers in Non-Small Cell Lung Cancer

Ergun, Sercan; Güney, Serkan; Temiz, Ebru; Petrović, Nina; Gunes, Sezgin

(2019)

TY  - JOUR
AU  - Ergun, Sercan
AU  - Güney, Serkan
AU  - Temiz, Ebru
AU  - Petrović, Nina
AU  - Gunes, Sezgin
PY  - 2019
UR  - http://www.eurekaselect.com/163857/article
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8099
AB  - Background: In recent years, targeted cancer treatment methods at various molecular levels have been developed for Non-Small Cell Lung Cancer (NSCLC), one of two major subtypes of lung cancer. miRNAbased clinical trials are currently the preferred targeted therapeutic strategy. Also, ceRNAs (competing endogenous RNA) would be the newest and the most effective approach to uncover novel interactions between mRNAs and miRNAs in NSCLC carcinogenesis. There are many factors influencing the efficiency of a miRNA to suppress or silence translation of the target mRNA. The most effective event is the presence of other RNAs showing ceRNA activity. These RNAs contain binding sites for specific miRNAs and enable miRNAs to bind these pseudo targets, instead of the original binding sites on the target mRNA. Therefore, the mRNA of the target gene is less affected by this miRNA, while the amount of miRNA remains the same in the media. Method: For this project, we determined that five clinically important different oncogenes (PDL1, FGFR1, DDX3X, SLC1A5, FXR1) are involved in the pathogenesis of NSCLC. For this purpose, we transfected model NSCLC cell line, A549, with miRNAs (miR-150-5p, miR-15a-5p, miR-503-5p) targeting these oncogenes to investigate whether these oncogenes will be suppressed at the mRNA level and also how the suppression efficiency of these miRNA on the oncogenes will be affected by possible ceRNA (CNKSR3, POU2F1, HIPK2) activities. Results: miR-15a-5p was determined to have the most suppressive effect on the five genes and three potential ceRNAs (p<0.05). Furthermore, CNKSR3 was the ceRNA most affected by all three miRNAs (p<0.05). Conclusion: CNKSR3 was affected more than the oncogenes known to act on NSCLC and this might make it a stronger and novel marker for use in possible treatment regimens designed using miR-15a-5p silencing effect on oncogenes in NSCLC pathogenesis. According to the literature, this is the first study associating NSCLC with miR-15a-5p and CNKSR3. © 2018 Bentham Science Publishers.
T2  - Anti-Cancer Agents in Medicinal Chemistry
T1  - Significance of miR-15a-5p and CNKSR3 as Novel Prognostic Biomarkers in Non-Small Cell Lung Cancer
VL  - 18
IS  - 12
SP  - 1695
EP  - 1701
DO  - 10.2174/1871520618666180718100656
ER  - 
@article{
author = "Ergun, Sercan and Güney, Serkan and Temiz, Ebru and Petrović, Nina and Gunes, Sezgin",
year = "2019",
abstract = "Background: In recent years, targeted cancer treatment methods at various molecular levels have been developed for Non-Small Cell Lung Cancer (NSCLC), one of two major subtypes of lung cancer. miRNAbased clinical trials are currently the preferred targeted therapeutic strategy. Also, ceRNAs (competing endogenous RNA) would be the newest and the most effective approach to uncover novel interactions between mRNAs and miRNAs in NSCLC carcinogenesis. There are many factors influencing the efficiency of a miRNA to suppress or silence translation of the target mRNA. The most effective event is the presence of other RNAs showing ceRNA activity. These RNAs contain binding sites for specific miRNAs and enable miRNAs to bind these pseudo targets, instead of the original binding sites on the target mRNA. Therefore, the mRNA of the target gene is less affected by this miRNA, while the amount of miRNA remains the same in the media. Method: For this project, we determined that five clinically important different oncogenes (PDL1, FGFR1, DDX3X, SLC1A5, FXR1) are involved in the pathogenesis of NSCLC. For this purpose, we transfected model NSCLC cell line, A549, with miRNAs (miR-150-5p, miR-15a-5p, miR-503-5p) targeting these oncogenes to investigate whether these oncogenes will be suppressed at the mRNA level and also how the suppression efficiency of these miRNA on the oncogenes will be affected by possible ceRNA (CNKSR3, POU2F1, HIPK2) activities. Results: miR-15a-5p was determined to have the most suppressive effect on the five genes and three potential ceRNAs (p<0.05). Furthermore, CNKSR3 was the ceRNA most affected by all three miRNAs (p<0.05). Conclusion: CNKSR3 was affected more than the oncogenes known to act on NSCLC and this might make it a stronger and novel marker for use in possible treatment regimens designed using miR-15a-5p silencing effect on oncogenes in NSCLC pathogenesis. According to the literature, this is the first study associating NSCLC with miR-15a-5p and CNKSR3. © 2018 Bentham Science Publishers.",
journal = "Anti-Cancer Agents in Medicinal Chemistry",
title = "Significance of miR-15a-5p and CNKSR3 as Novel Prognostic Biomarkers in Non-Small Cell Lung Cancer",
volume = "18",
number = "12",
pages = "1695-1701",
doi = "10.2174/1871520618666180718100656"
}
Ergun, S., Güney, S., Temiz, E., Petrović, N.,& Gunes, S.. (2019). Significance of miR-15a-5p and CNKSR3 as Novel Prognostic Biomarkers in Non-Small Cell Lung Cancer. in Anti-Cancer Agents in Medicinal Chemistry, 18(12), 1695-1701.
https://doi.org/10.2174/1871520618666180718100656
Ergun S, Güney S, Temiz E, Petrović N, Gunes S. Significance of miR-15a-5p and CNKSR3 as Novel Prognostic Biomarkers in Non-Small Cell Lung Cancer. in Anti-Cancer Agents in Medicinal Chemistry. 2019;18(12):1695-1701.
doi:10.2174/1871520618666180718100656 .
Ergun, Sercan, Güney, Serkan, Temiz, Ebru, Petrović, Nina, Gunes, Sezgin, "Significance of miR-15a-5p and CNKSR3 as Novel Prognostic Biomarkers in Non-Small Cell Lung Cancer" in Anti-Cancer Agents in Medicinal Chemistry, 18, no. 12 (2019):1695-1701,
https://doi.org/10.2174/1871520618666180718100656 . .
12
14
12

Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits

Baćević, Miljana; Brković, Božidar; Lambert, France; Đukić, Ljiljana; Petrović, Nina; Roganović, Jelena

(2019)

TY  - JOUR
AU  - Baćević, Miljana
AU  - Brković, Božidar
AU  - Lambert, France
AU  - Đukić, Ljiljana
AU  - Petrović, Nina
AU  - Roganović, Jelena
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0003996919301232
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8175
AB  - Objective: Leukocyte- and platelet-rich fibrin (L-PRF) represents a natural, low-cost product which may promote tissue healing by mechanisms not fully elucidated. Diabetes mellitus (DM) disrupts bone healing by inducing inflammation and oxidative stress (OS), mechanisms regulated by microRNAs (miRs). The aim of the present study was to investigate the microRNA-21 (miR-21) involvement in diabetic bone regeneration using L-PRF alone or in combination with a standard grafting material. Design: After the induction of diabetes (alloxan 100 mg/kg), four cranial osteotomies were made in diabetic (n = 12) and non-diabetic (n = 12) rabbits: one was left empty and the remaining three were grafted with L-PRF, bovine hydroxyapatite (Bio-Oss®) and L-PRF + Bio-Oss®. Two and eight weeks postoperatively, the samples were harvested for miR-21 expression (Real-time RT-PCR) and enzyme-linked immunosorbent assay analyses. Results: Diabetic rabbits showed decreased miR-21 and matrix metalloproteinase-9 (MMP-9) protein expression while increased malondialdehyde (MDA) levels two weeks postoperatively; however, there were no significant differences in miR-21 and MMP-9 levels between diabetic and non-diabetic rabbits in samples taken eight weeks postoperatively. Application of L-PRF and L-PRF + Bio-Oss® improved miR-21 and MMP-9 and decreased MDA levels while Bio-Oss® alone enhanced superoxide dismutase (SOD) activity levels in diabetic rabbits. Conclusion: L-PRF alone or in combination with bovine hydroxyapatite as bone graft could be beneficial in DM since it seems to improve inflammation-modulatory miR-21 expression and decreases oxidative stress. © 2019 Elsevier Ltd
T2  - Archives of Oral Biology
T1  - Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits
VL  - 102
SP  - 231
EP  - 237
DO  - 10.1016/j.archoralbio.2019.05.005
ER  - 
@article{
author = "Baćević, Miljana and Brković, Božidar and Lambert, France and Đukić, Ljiljana and Petrović, Nina and Roganović, Jelena",
year = "2019",
abstract = "Objective: Leukocyte- and platelet-rich fibrin (L-PRF) represents a natural, low-cost product which may promote tissue healing by mechanisms not fully elucidated. Diabetes mellitus (DM) disrupts bone healing by inducing inflammation and oxidative stress (OS), mechanisms regulated by microRNAs (miRs). The aim of the present study was to investigate the microRNA-21 (miR-21) involvement in diabetic bone regeneration using L-PRF alone or in combination with a standard grafting material. Design: After the induction of diabetes (alloxan 100 mg/kg), four cranial osteotomies were made in diabetic (n = 12) and non-diabetic (n = 12) rabbits: one was left empty and the remaining three were grafted with L-PRF, bovine hydroxyapatite (Bio-Oss®) and L-PRF + Bio-Oss®. Two and eight weeks postoperatively, the samples were harvested for miR-21 expression (Real-time RT-PCR) and enzyme-linked immunosorbent assay analyses. Results: Diabetic rabbits showed decreased miR-21 and matrix metalloproteinase-9 (MMP-9) protein expression while increased malondialdehyde (MDA) levels two weeks postoperatively; however, there were no significant differences in miR-21 and MMP-9 levels between diabetic and non-diabetic rabbits in samples taken eight weeks postoperatively. Application of L-PRF and L-PRF + Bio-Oss® improved miR-21 and MMP-9 and decreased MDA levels while Bio-Oss® alone enhanced superoxide dismutase (SOD) activity levels in diabetic rabbits. Conclusion: L-PRF alone or in combination with bovine hydroxyapatite as bone graft could be beneficial in DM since it seems to improve inflammation-modulatory miR-21 expression and decreases oxidative stress. © 2019 Elsevier Ltd",
journal = "Archives of Oral Biology",
title = "Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits",
volume = "102",
pages = "231-237",
doi = "10.1016/j.archoralbio.2019.05.005"
}
Baćević, M., Brković, B., Lambert, F., Đukić, L., Petrović, N.,& Roganović, J.. (2019). Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits. in Archives of Oral Biology, 102, 231-237.
https://doi.org/10.1016/j.archoralbio.2019.05.005
Baćević M, Brković B, Lambert F, Đukić L, Petrović N, Roganović J. Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits. in Archives of Oral Biology. 2019;102:231-237.
doi:10.1016/j.archoralbio.2019.05.005 .
Baćević, Miljana, Brković, Božidar, Lambert, France, Đukić, Ljiljana, Petrović, Nina, Roganović, Jelena, "Leukocyte- and platelet-rich fibrin as graft material improves microRNA-21 expression and decreases oxidative stress in the calvarial defects of diabetic rabbits" in Archives of Oral Biology, 102 (2019):231-237,
https://doi.org/10.1016/j.archoralbio.2019.05.005 . .
4
3
3

Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study

Kopčalić, Katarina; Petrović, Nina; Stanojković, Tatjana P.; Stanković, Vesna; Bukumirić, Zoran; Roganović, Jelena; Mališić, Emina; Nikitović, Marina

(2019)

TY  - JOUR
AU  - Kopčalić, Katarina
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Stanković, Vesna
AU  - Bukumirić, Zoran
AU  - Roganović, Jelena
AU  - Mališić, Emina
AU  - Nikitović, Marina
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8444
AB  - Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs. © 2018 Elsevier GmbH
T2  - Pathology - Research and Practice
T1  - Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study
VL  - 215
IS  - 4
SP  - 626
EP  - 631
DO  - 10.1016/j.prp.2018.12.007
ER  - 
@article{
author = "Kopčalić, Katarina and Petrović, Nina and Stanojković, Tatjana P. and Stanković, Vesna and Bukumirić, Zoran and Roganović, Jelena and Mališić, Emina and Nikitović, Marina",
year = "2019",
abstract = "Introduction: Nearly sixty percent of patients with prostate cancer (PCa) undergo radiation therapy (RT). During the course of treatment patients may experience normal tissue reactions. It is a well established fact that genetic and epigenetic mechanisms, such as microRNA (miRNA) level changes might be associated with radiotoxicity, as a response to irradiation. Materials and methods: This is the first study that has investigated levels of radiosensory miRNAs in association with acute genitourinary radiotoxicity extracted from peripheral blood mononuclear cells (PBCs), in three points; before RT (BRT), after RT (ART) and on the first control examination (FCONT). We measured levels of miR-21/146a/155 expression by quantitative real-time PCR (qRT-PCR), comparative ΔΔCt method, in fifteen patients with localized prostate cancer, treated with three-dimensional conformal radiotherapy (3DCRT). Nine subjects have experienced acute genitourinary (GU) radiotoxicity whereas six where without GU radiotoxicity. Results: Firstly, we detected the highest levels of miR-21 in ART group (p = 0.043) in the patients with acute GU radiotoxicity. Secondly, we found trend towards higher miR-21 levels and significantly higher levels of miR-146a/155 within the patients with acute GU toxicity than in patients without (p = 0.068, p = 0.016, and p = 0.010, respectively). Thirdly, we detected significant change in miR-146a/155 levels within the patients without acute GU radiotoxicity during RT p = 0.042, and p = 0.041, respectively). Conclusion: miR-21/146a/155 might be useful potential factors of radiosensitivity and acute genitourinary radiotoxicity in prostate cancer patients. miRNA might have great potential as predictors of various pathological conditions extracted from PBMCs. © 2018 Elsevier GmbH",
journal = "Pathology - Research and Practice",
title = "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study",
volume = "215",
number = "4",
pages = "626-631",
doi = "10.1016/j.prp.2018.12.007"
}
Kopčalić, K., Petrović, N., Stanojković, T. P., Stanković, V., Bukumirić, Z., Roganović, J., Mališić, E.,& Nikitović, M.. (2019). Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology - Research and Practice, 215(4), 626-631.
https://doi.org/10.1016/j.prp.2018.12.007
Kopčalić K, Petrović N, Stanojković TP, Stanković V, Bukumirić Z, Roganović J, Mališić E, Nikitović M. Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study. in Pathology - Research and Practice. 2019;215(4):626-631.
doi:10.1016/j.prp.2018.12.007 .
Kopčalić, Katarina, Petrović, Nina, Stanojković, Tatjana P., Stanković, Vesna, Bukumirić, Zoran, Roganović, Jelena, Mališić, Emina, Nikitović, Marina, "Association between miR-21/146a/155 level changes and acute genitourinary radiotoxicity in prostate cancer patients: A pilot study" in Pathology - Research and Practice, 215, no. 4 (2019):626-631,
https://doi.org/10.1016/j.prp.2018.12.007 . .
8
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7

Expression of VHL tumor suppressor mRNA and miR-92a in papillary thyroid carcinoma and their correlation with clinical and pathological parameters

Todorović, Lidija; Stanojević, Boban; Mandušić, Vesna; Petrović, Nina; Zivaljevic, Vladan; Paunovic, Ivan; Diklić, Aleksandar; Saenko, Vladimir; Yamashita, Shunichi

(2018)

TY  - JOUR
AU  - Todorović, Lidija
AU  - Stanojević, Boban
AU  - Mandušić, Vesna
AU  - Petrović, Nina
AU  - Zivaljevic, Vladan
AU  - Paunovic, Ivan
AU  - Diklić, Aleksandar
AU  - Saenko, Vladimir
AU  - Yamashita, Shunichi
PY  - 2018
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1944
AB  - A growing body of evidence suggests a role of the von Hippel-Lindau (VHL) tumor suppressor gene in the progression of papillary thyroid carcinoma (PTC). Our previous study of VHL in PTCs showed that lower VHL expression was associated with aggressive tumor features, but we found no evidence for VHL downregulation through common genetic or epigenetic modifications. Several studies pointed to a role of microRNA-92a (miR-92a) in the regulation of VHL expression in different cancers. In the present study, we examined the expression levels of VHL mRNA and miR-92a in 42 pairs of PTCs and matched non-tumor thyroid tissues by means of quantitative RT-PCR. We explored the correlation between them and their association with clinicopathological parameters. The results revealed that both VHL and miR-92a were either up-or downregulated in PTCs compared to corresponding non-tumor tissues. On univariate analysis, lower VHL levels were significantly associated with extrathyroid spread (P = 0.022) and capsular invasion (P = 0.032). Multivariate analysis confirmed the association of low VHL with extrathyroid spread (OR 0.246, 95% CI 0.069-0.872, P = 0.038). Higher miR-92a among PTC tissues associated with the presence of nodal metastases (univariate analysis: P = 0.012; multivariate: OR 4.703, 95% CI 1.109-19.938, P = 0.036). A negative correlation between VHL and miR-92a was observed in a subgroup of PTCs having vascular invasion (P = 0.033, r = -0.673). The data here reported demonstrate that the expression of both VHL and miR-92a is deregulated in PTC tissues and that in some PTCs they may have opposite roles. These roles, as well as their diagnostic and/or prognostic utility, remain to be clarified.
T2  - Medical Oncology
T1  - Expression of VHL tumor suppressor mRNA and miR-92a in papillary thyroid carcinoma and their correlation with clinical and pathological parameters
VL  - 35
IS  - 2
DO  - 10.1007/s12032-017-1066-3
ER  - 
@article{
author = "Todorović, Lidija and Stanojević, Boban and Mandušić, Vesna and Petrović, Nina and Zivaljevic, Vladan and Paunovic, Ivan and Diklić, Aleksandar and Saenko, Vladimir and Yamashita, Shunichi",
year = "2018",
abstract = "A growing body of evidence suggests a role of the von Hippel-Lindau (VHL) tumor suppressor gene in the progression of papillary thyroid carcinoma (PTC). Our previous study of VHL in PTCs showed that lower VHL expression was associated with aggressive tumor features, but we found no evidence for VHL downregulation through common genetic or epigenetic modifications. Several studies pointed to a role of microRNA-92a (miR-92a) in the regulation of VHL expression in different cancers. In the present study, we examined the expression levels of VHL mRNA and miR-92a in 42 pairs of PTCs and matched non-tumor thyroid tissues by means of quantitative RT-PCR. We explored the correlation between them and their association with clinicopathological parameters. The results revealed that both VHL and miR-92a were either up-or downregulated in PTCs compared to corresponding non-tumor tissues. On univariate analysis, lower VHL levels were significantly associated with extrathyroid spread (P = 0.022) and capsular invasion (P = 0.032). Multivariate analysis confirmed the association of low VHL with extrathyroid spread (OR 0.246, 95% CI 0.069-0.872, P = 0.038). Higher miR-92a among PTC tissues associated with the presence of nodal metastases (univariate analysis: P = 0.012; multivariate: OR 4.703, 95% CI 1.109-19.938, P = 0.036). A negative correlation between VHL and miR-92a was observed in a subgroup of PTCs having vascular invasion (P = 0.033, r = -0.673). The data here reported demonstrate that the expression of both VHL and miR-92a is deregulated in PTC tissues and that in some PTCs they may have opposite roles. These roles, as well as their diagnostic and/or prognostic utility, remain to be clarified.",
journal = "Medical Oncology",
title = "Expression of VHL tumor suppressor mRNA and miR-92a in papillary thyroid carcinoma and their correlation with clinical and pathological parameters",
volume = "35",
number = "2",
doi = "10.1007/s12032-017-1066-3"
}
Todorović, L., Stanojević, B., Mandušić, V., Petrović, N., Zivaljevic, V., Paunovic, I., Diklić, A., Saenko, V.,& Yamashita, S.. (2018). Expression of VHL tumor suppressor mRNA and miR-92a in papillary thyroid carcinoma and their correlation with clinical and pathological parameters. in Medical Oncology, 35(2).
https://doi.org/10.1007/s12032-017-1066-3
Todorović L, Stanojević B, Mandušić V, Petrović N, Zivaljevic V, Paunovic I, Diklić A, Saenko V, Yamashita S. Expression of VHL tumor suppressor mRNA and miR-92a in papillary thyroid carcinoma and their correlation with clinical and pathological parameters. in Medical Oncology. 2018;35(2).
doi:10.1007/s12032-017-1066-3 .
Todorović, Lidija, Stanojević, Boban, Mandušić, Vesna, Petrović, Nina, Zivaljevic, Vladan, Paunovic, Ivan, Diklić, Aleksandar, Saenko, Vladimir, Yamashita, Shunichi, "Expression of VHL tumor suppressor mRNA and miR-92a in papillary thyroid carcinoma and their correlation with clinical and pathological parameters" in Medical Oncology, 35, no. 2 (2018),
https://doi.org/10.1007/s12032-017-1066-3 . .
10
5
5
6

Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies

Jakovljević, Katarina; Joksović, Milan D.; Matić, Ivana Z.; Petrović, Nina; Stanojković, Tatjana P.; Sladić, Dušan M.; Vujčić, Miroslava T.; Janović, Barbara S.; Joksović, Ljubinka G.; Trifunović, Snežana; Marković, Violeta

(2018)

TY  - JOUR
AU  - Jakovljević, Katarina
AU  - Joksović, Milan D.
AU  - Matić, Ivana Z.
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Sladić, Dušan M.
AU  - Vujčić, Miroslava T.
AU  - Janović, Barbara S.
AU  - Joksović, Ljubinka G.
AU  - Trifunović, Snežana
AU  - Marković, Violeta
PY  - 2018
UR  - http://xlink.rsc.org/?DOI=C8MD00316E
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7928
AB  - Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.
T2  - MedChemComm
T1  - Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies
VL  - 9
IS  - 10
SP  - 1679
EP  - 1697
DO  - 10.1039/C8MD00316E
ER  - 
@article{
author = "Jakovljević, Katarina and Joksović, Milan D. and Matić, Ivana Z. and Petrović, Nina and Stanojković, Tatjana P. and Sladić, Dušan M. and Vujčić, Miroslava T. and Janović, Barbara S. and Joksović, Ljubinka G. and Trifunović, Snežana and Marković, Violeta",
year = "2018",
abstract = "Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.",
journal = "MedChemComm",
title = "Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies",
volume = "9",
number = "10",
pages = "1679-1697",
doi = "10.1039/C8MD00316E"
}
Jakovljević, K., Joksović, M. D., Matić, I. Z., Petrović, N., Stanojković, T. P., Sladić, D. M., Vujčić, M. T., Janović, B. S., Joksović, L. G., Trifunović, S.,& Marković, V.. (2018). Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in MedChemComm, 9(10), 1679-1697.
https://doi.org/10.1039/C8MD00316E
Jakovljević K, Joksović MD, Matić IZ, Petrović N, Stanojković TP, Sladić DM, Vujčić MT, Janović BS, Joksović LG, Trifunović S, Marković V. Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in MedChemComm. 2018;9(10):1679-1697.
doi:10.1039/C8MD00316E .
Jakovljević, Katarina, Joksović, Milan D., Matić, Ivana Z., Petrović, Nina, Stanojković, Tatjana P., Sladić, Dušan M., Vujčić, Miroslava T., Janović, Barbara S., Joksović, Ljubinka G., Trifunović, Snežana, Marković, Violeta, "Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies" in MedChemComm, 9, no. 10 (2018):1679-1697,
https://doi.org/10.1039/C8MD00316E . .
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9
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10

Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents

Stanojković, Tatjana P.; Marković, Violeta; Matić, Ivana Z.; Mladenović, Milan P.; Petrović, Nina; Krivokuća, Ana M.; Petković, Miloš R.; Joksović, Milan D.

(2018)

TY  - JOUR
AU  - Stanojković, Tatjana P.
AU  - Marković, Violeta
AU  - Matić, Ivana Z.
AU  - Mladenović, Milan P.
AU  - Petrović, Nina
AU  - Krivokuća, Ana M.
AU  - Petković, Miloš R.
AU  - Joksović, Milan D.
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0960894X18305493
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7815
AB  - A series of 23 novel anthraquinone-chalcone hybrids containing amide function was synthesized and structurally characterized. Sixteen compounds exerted strong cytotoxic activities against K562, Jurkat and HL-60 leukemia cell lines and significantly lower cytotoxic effects against normal MRC-5 cells, indicating very high selectivity in their anticancer action. The compounds 6g, 6u and 6v activate apoptosis in K562 cells through the extrinsic and intrinsic apoptotic pathway. The compound 6e triggered apoptosis in K562 cells only through the extrinsic apoptotic pathway. Treatment of K562 cells with each of these four compounds caused decrease in the expression levels of MMP2, MMP9, and VEGF, suggesting their anti-invasive, antimetastatic and antiangiogenic properties. The compounds 6g and 6v downregulated expression levels of miR-155 in K562 cells, while compounds 6e and 6u upregulated miR-155 levels in treated cells, in comparison with control cells. The structure-based 3-D QSAR models for 6f, 6e, 6i and 6l describe pro-apoptotic activity against caspase-3. © 2018 Elsevier Ltd
T2  - Bioorganic & Medicinal Chemistry Letters
T1  - Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents
VL  - 28
IS  - 15
SP  - 2593
EP  - 2598
DO  - 10.1016/j.bmcl.2018.06.048
ER  - 
@article{
author = "Stanojković, Tatjana P. and Marković, Violeta and Matić, Ivana Z. and Mladenović, Milan P. and Petrović, Nina and Krivokuća, Ana M. and Petković, Miloš R. and Joksović, Milan D.",
year = "2018",
abstract = "A series of 23 novel anthraquinone-chalcone hybrids containing amide function was synthesized and structurally characterized. Sixteen compounds exerted strong cytotoxic activities against K562, Jurkat and HL-60 leukemia cell lines and significantly lower cytotoxic effects against normal MRC-5 cells, indicating very high selectivity in their anticancer action. The compounds 6g, 6u and 6v activate apoptosis in K562 cells through the extrinsic and intrinsic apoptotic pathway. The compound 6e triggered apoptosis in K562 cells only through the extrinsic apoptotic pathway. Treatment of K562 cells with each of these four compounds caused decrease in the expression levels of MMP2, MMP9, and VEGF, suggesting their anti-invasive, antimetastatic and antiangiogenic properties. The compounds 6g and 6v downregulated expression levels of miR-155 in K562 cells, while compounds 6e and 6u upregulated miR-155 levels in treated cells, in comparison with control cells. The structure-based 3-D QSAR models for 6f, 6e, 6i and 6l describe pro-apoptotic activity against caspase-3. © 2018 Elsevier Ltd",
journal = "Bioorganic & Medicinal Chemistry Letters",
title = "Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents",
volume = "28",
number = "15",
pages = "2593-2598",
doi = "10.1016/j.bmcl.2018.06.048"
}
Stanojković, T. P., Marković, V., Matić, I. Z., Mladenović, M. P., Petrović, N., Krivokuća, A. M., Petković, M. R.,& Joksović, M. D.. (2018). Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents. in Bioorganic & Medicinal Chemistry Letters, 28(15), 2593-2598.
https://doi.org/10.1016/j.bmcl.2018.06.048
Stanojković TP, Marković V, Matić IZ, Mladenović MP, Petrović N, Krivokuća AM, Petković MR, Joksović MD. Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents. in Bioorganic & Medicinal Chemistry Letters. 2018;28(15):2593-2598.
doi:10.1016/j.bmcl.2018.06.048 .
Stanojković, Tatjana P., Marković, Violeta, Matić, Ivana Z., Mladenović, Milan P., Petrović, Nina, Krivokuća, Ana M., Petković, Miloš R., Joksović, Milan D., "Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents" in Bioorganic & Medicinal Chemistry Letters, 28, no. 15 (2018):2593-2598,
https://doi.org/10.1016/j.bmcl.2018.06.048 . .
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8
9

Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221

Panić, Anastasija; Stanimirović, Julijana; Obradović, Milan M.; Sudar-Milovanović, Emina; Perović, Milan; Lačković, Milena; Petrović, Nina; Isenović, Esma R.

(2018)

TY  - JOUR
AU  - Panić, Anastasija
AU  - Stanimirović, Julijana
AU  - Obradović, Milan M.
AU  - Sudar-Milovanović, Emina
AU  - Perović, Milan
AU  - Lačković, Milena
AU  - Petrović, Nina
AU  - Isenović, Esma R.
PY  - 2018
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8399
AB  - Purpose: This study aimed to investigate in vivo effects of estradiol on the regulation of hepatic inducible nitric oxide synthase (iNOS) expression in the high fat (HF) diet-induced obesity. Also, we aimed to investigate whether activation of the extracellular signal-regulated kinase (ERK1/2), adenosine monophosphate-activated protein kinase (AMPK), Src kinase, and miR-221 is involved in estradiol-mediated regulation of iNOS in the liver of obese male Wistar rats. Male Wistar rats were fed a standard laboratory diet or a HF diet for 10 weeks. Half of HF rats were treated with estradiol intraperitoneally (40 μg/kg), whereas the other half were placebo-treated 24 H before euthanasia. Results show that estradiol treatment of HF rats decreased hepatic iNOS mRNA (P < 0.05) and protein expression (P < 0.01), the protein levels of p65 subunit of nuclear factor κB (P < 0.05) and ERα (P < 0.05), ERK1/2 phosphorylation (P < 0.001), and ERα/Src kinase association (P < 0.05). By contrast, hepatic Src protein level (P < 0.05), AMPKα phosphorylation (P < 0.05), and miR-221 expression (P < 0.05) were increased in HF rats after estradiol treatment. Our results indicate that estradiol in vivo regulates hepatic iNOS expression in obese rats via molecular mechanisms involving ERK1/2, AMPK, Src, and miR-221 signaling. © 2018 International Union of Biochemistry and Molecular Biology, Inc.
T2  - Biotechnology and Applied Biochemistry
T1  - Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221
VL  - 65
IS  - 6
SP  - 797
EP  - 806
DO  - 10.1002/bab.1680
ER  - 
@article{
author = "Panić, Anastasija and Stanimirović, Julijana and Obradović, Milan M. and Sudar-Milovanović, Emina and Perović, Milan and Lačković, Milena and Petrović, Nina and Isenović, Esma R.",
year = "2018",
abstract = "Purpose: This study aimed to investigate in vivo effects of estradiol on the regulation of hepatic inducible nitric oxide synthase (iNOS) expression in the high fat (HF) diet-induced obesity. Also, we aimed to investigate whether activation of the extracellular signal-regulated kinase (ERK1/2), adenosine monophosphate-activated protein kinase (AMPK), Src kinase, and miR-221 is involved in estradiol-mediated regulation of iNOS in the liver of obese male Wistar rats. Male Wistar rats were fed a standard laboratory diet or a HF diet for 10 weeks. Half of HF rats were treated with estradiol intraperitoneally (40 μg/kg), whereas the other half were placebo-treated 24 H before euthanasia. Results show that estradiol treatment of HF rats decreased hepatic iNOS mRNA (P < 0.05) and protein expression (P < 0.01), the protein levels of p65 subunit of nuclear factor κB (P < 0.05) and ERα (P < 0.05), ERK1/2 phosphorylation (P < 0.001), and ERα/Src kinase association (P < 0.05). By contrast, hepatic Src protein level (P < 0.05), AMPKα phosphorylation (P < 0.05), and miR-221 expression (P < 0.05) were increased in HF rats after estradiol treatment. Our results indicate that estradiol in vivo regulates hepatic iNOS expression in obese rats via molecular mechanisms involving ERK1/2, AMPK, Src, and miR-221 signaling. © 2018 International Union of Biochemistry and Molecular Biology, Inc.",
journal = "Biotechnology and Applied Biochemistry",
title = "Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221",
volume = "65",
number = "6",
pages = "797-806",
doi = "10.1002/bab.1680"
}
Panić, A., Stanimirović, J., Obradović, M. M., Sudar-Milovanović, E., Perović, M., Lačković, M., Petrović, N.,& Isenović, E. R.. (2018). Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221. in Biotechnology and Applied Biochemistry, 65(6), 797-806.
https://doi.org/10.1002/bab.1680
Panić A, Stanimirović J, Obradović MM, Sudar-Milovanović E, Perović M, Lačković M, Petrović N, Isenović ER. Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221. in Biotechnology and Applied Biochemistry. 2018;65(6):797-806.
doi:10.1002/bab.1680 .
Panić, Anastasija, Stanimirović, Julijana, Obradović, Milan M., Sudar-Milovanović, Emina, Perović, Milan, Lačković, Milena, Petrović, Nina, Isenović, Esma R., "Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221" in Biotechnology and Applied Biochemistry, 65, no. 6 (2018):797-806,
https://doi.org/10.1002/bab.1680 . .
4
3
3

17β-estradiol inhibits hepatic iNOS via the activation of the estrogen receptor ER-α and inhibition of erk1/2-mir-221 axis

Panić, Anastasija; Stanimirović, Julijana; Obradović, Milan M.; Zafirović, Sonja; Sudar-Milovanović, Emina; Petrović, Nina; Isenović, Esma R.

(2018)

TY  - JOUR
AU  - Panić, Anastasija
AU  - Stanimirović, Julijana
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Sudar-Milovanović, Emina
AU  - Petrović, Nina
AU  - Isenović, Esma R.
PY  - 2018
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8013
AB  - 17β-Estradiol (E2) is known to negatively regulate inducible nitric oxide (NO) synthase (iNOS) expression via estrogen receptor alpha (ER-α) activation in aortic vascular smooth muscle cells.Therefore, we sought to determine whether E2 can inhibit iNOS in vivo in hepatic tissue via the activation of ER-α and whether extracellular signal-regulated kinases 1/2 (ERK1/2)-miR-221 axis is involved in this process. Male Wistar rats were treated with a bolus injection of E2 intraperitoneally (40 μg/kg), and 24 hours after treatment the animals were sacrificed and the livers excised. The protein levels of iNOS, p50 and p65 subunits of nuclear factor κB (NFκB), ERα, ERK1/2 and protein kinase B (Akt), as well as the association of ERα/Src in liver lysates were assessed by Western blot. The expression of hepatic miR-221 was analyzed by qRT-PCR. Results show that E2 reduced hepatic iNOS protein expression (p less than 0.01), the protein level of ERα (p less than 0.05), ERK1/2 (p less than 0.05), Akt phosphorylation (p less than 0.001) and miR-221 expression (p less than 0.05). In contrast, hepatic ERα/Src kinase association level (p less than 0.05) increased after E2 treatment. Our results indicate that E2 inhibits hepatic iNOS via molecular mechanisms involving the activation of the ER-α and inhibition of ERK1/2-miR-221 axis.
T2  - Journal of biological regulators and homeostatic agents
T1  - 17β-estradiol inhibits hepatic iNOS via the activation of the estrogen receptor ER-α and inhibition of erk1/2-mir-221 axis
VL  - 32
IS  - 6
SP  - 1369
EP  - 1377
ER  - 
@article{
author = "Panić, Anastasija and Stanimirović, Julijana and Obradović, Milan M. and Zafirović, Sonja and Sudar-Milovanović, Emina and Petrović, Nina and Isenović, Esma R.",
year = "2018",
abstract = "17β-Estradiol (E2) is known to negatively regulate inducible nitric oxide (NO) synthase (iNOS) expression via estrogen receptor alpha (ER-α) activation in aortic vascular smooth muscle cells.Therefore, we sought to determine whether E2 can inhibit iNOS in vivo in hepatic tissue via the activation of ER-α and whether extracellular signal-regulated kinases 1/2 (ERK1/2)-miR-221 axis is involved in this process. Male Wistar rats were treated with a bolus injection of E2 intraperitoneally (40 μg/kg), and 24 hours after treatment the animals were sacrificed and the livers excised. The protein levels of iNOS, p50 and p65 subunits of nuclear factor κB (NFκB), ERα, ERK1/2 and protein kinase B (Akt), as well as the association of ERα/Src in liver lysates were assessed by Western blot. The expression of hepatic miR-221 was analyzed by qRT-PCR. Results show that E2 reduced hepatic iNOS protein expression (p less than 0.01), the protein level of ERα (p less than 0.05), ERK1/2 (p less than 0.05), Akt phosphorylation (p less than 0.001) and miR-221 expression (p less than 0.05). In contrast, hepatic ERα/Src kinase association level (p less than 0.05) increased after E2 treatment. Our results indicate that E2 inhibits hepatic iNOS via molecular mechanisms involving the activation of the ER-α and inhibition of ERK1/2-miR-221 axis.",
journal = "Journal of biological regulators and homeostatic agents",
title = "17β-estradiol inhibits hepatic iNOS via the activation of the estrogen receptor ER-α and inhibition of erk1/2-mir-221 axis",
volume = "32",
number = "6",
pages = "1369-1377"
}
Panić, A., Stanimirović, J., Obradović, M. M., Zafirović, S., Sudar-Milovanović, E., Petrović, N.,& Isenović, E. R.. (2018). 17β-estradiol inhibits hepatic iNOS via the activation of the estrogen receptor ER-α and inhibition of erk1/2-mir-221 axis. in Journal of biological regulators and homeostatic agents, 32(6), 1369-1377.
Panić A, Stanimirović J, Obradović MM, Zafirović S, Sudar-Milovanović E, Petrović N, Isenović ER. 17β-estradiol inhibits hepatic iNOS via the activation of the estrogen receptor ER-α and inhibition of erk1/2-mir-221 axis. in Journal of biological regulators and homeostatic agents. 2018;32(6):1369-1377..
Panić, Anastasija, Stanimirović, Julijana, Obradović, Milan M., Zafirović, Sonja, Sudar-Milovanović, Emina, Petrović, Nina, Isenović, Esma R., "17β-estradiol inhibits hepatic iNOS via the activation of the estrogen receptor ER-α and inhibition of erk1/2-mir-221 axis" in Journal of biological regulators and homeostatic agents, 32, no. 6 (2018):1369-1377.
3

Hormonal status and distribution of the ABO system phenotypic groups in menopausal and postmenopausal women with breast tumors

Koridze, Marina; Baratashvili, Davit; Khukhunaishvili, Rusudan; Nakashidze, Irina; Petrović, Nina; Nagervadze, Marina; Zosidze, Nato; Tskvitinidze, Sophiko; Kotrikadze, Nanuli; Ahmad, Sarfraz

(2018)

TY  - JOUR
AU  - Koridze, Marina
AU  - Baratashvili, Davit
AU  - Khukhunaishvili, Rusudan
AU  - Nakashidze, Irina
AU  - Petrović, Nina
AU  - Nagervadze, Marina
AU  - Zosidze, Nato
AU  - Tskvitinidze, Sophiko
AU  - Kotrikadze, Nanuli
AU  - Ahmad, Sarfraz
PY  - 2018
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1952
AB  - Breast cancer is one of the most frequent neoplastic diseases within the female population worldwide. Hormonal imbalance and the ABO system group antigens are among the numerous risk-factors which provoke the development of breast benign and malignant tumors. Here, we have investigated the following sex-steroid hormones: estradiol (E2), progesterone (P), testosterone (T)), non-sex hormones (thyroxin (fT4), thyroid-stimulating hormone (TSH) and prolactin (PRL), and the distribution of the ABO system phenotypic groups in the menopausal and postmenopausal women with breast tumors (benign, malignant). Enzyme-linked immunosorbent assay (ELISA) was used for quantitative determination of hormones. The immune-serological methods were used for investigation of the ABO system phenotypic groups. Our present investigations in menopausal and postmenopausal women with breast tumors have revealed significantly higher expression of sex-steroid hormone estradiol, but decreased progesterone, and also significantly increased testosterone levels. Thyroid gland revealed hypofunction, which confirms the decrease of thyroxin, and increase of prolactin and TSH in the blood. According to our findings, carriers of A(II) phenotypic groups showed high risk for breast tumors development in women during both stages, menopausal and postmenopausal.
T2  - Indian Journal of Experimental Biology
T1  - Hormonal status and distribution of the ABO system phenotypic groups in menopausal and postmenopausal women with breast tumors
VL  - 56
IS  - 2
SP  - 93
EP  - 100
ER  - 
@article{
author = "Koridze, Marina and Baratashvili, Davit and Khukhunaishvili, Rusudan and Nakashidze, Irina and Petrović, Nina and Nagervadze, Marina and Zosidze, Nato and Tskvitinidze, Sophiko and Kotrikadze, Nanuli and Ahmad, Sarfraz",
year = "2018",
abstract = "Breast cancer is one of the most frequent neoplastic diseases within the female population worldwide. Hormonal imbalance and the ABO system group antigens are among the numerous risk-factors which provoke the development of breast benign and malignant tumors. Here, we have investigated the following sex-steroid hormones: estradiol (E2), progesterone (P), testosterone (T)), non-sex hormones (thyroxin (fT4), thyroid-stimulating hormone (TSH) and prolactin (PRL), and the distribution of the ABO system phenotypic groups in the menopausal and postmenopausal women with breast tumors (benign, malignant). Enzyme-linked immunosorbent assay (ELISA) was used for quantitative determination of hormones. The immune-serological methods were used for investigation of the ABO system phenotypic groups. Our present investigations in menopausal and postmenopausal women with breast tumors have revealed significantly higher expression of sex-steroid hormone estradiol, but decreased progesterone, and also significantly increased testosterone levels. Thyroid gland revealed hypofunction, which confirms the decrease of thyroxin, and increase of prolactin and TSH in the blood. According to our findings, carriers of A(II) phenotypic groups showed high risk for breast tumors development in women during both stages, menopausal and postmenopausal.",
journal = "Indian Journal of Experimental Biology",
title = "Hormonal status and distribution of the ABO system phenotypic groups in menopausal and postmenopausal women with breast tumors",
volume = "56",
number = "2",
pages = "93-100"
}
Koridze, M., Baratashvili, D., Khukhunaishvili, R., Nakashidze, I., Petrović, N., Nagervadze, M., Zosidze, N., Tskvitinidze, S., Kotrikadze, N.,& Ahmad, S.. (2018). Hormonal status and distribution of the ABO system phenotypic groups in menopausal and postmenopausal women with breast tumors. in Indian Journal of Experimental Biology, 56(2), 93-100.
Koridze M, Baratashvili D, Khukhunaishvili R, Nakashidze I, Petrović N, Nagervadze M, Zosidze N, Tskvitinidze S, Kotrikadze N, Ahmad S. Hormonal status and distribution of the ABO system phenotypic groups in menopausal and postmenopausal women with breast tumors. in Indian Journal of Experimental Biology. 2018;56(2):93-100..
Koridze, Marina, Baratashvili, Davit, Khukhunaishvili, Rusudan, Nakashidze, Irina, Petrović, Nina, Nagervadze, Marina, Zosidze, Nato, Tskvitinidze, Sophiko, Kotrikadze, Nanuli, Ahmad, Sarfraz, "Hormonal status and distribution of the ABO system phenotypic groups in menopausal and postmenopausal women with breast tumors" in Indian Journal of Experimental Biology, 56, no. 2 (2018):93-100.

MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients

Radović, Nikola; Nikolić Jakoba, Nataša; Petrović, Nina; Milosavljević, Aleksandra; Brković, Božidar; Roganović, Jelena

(2018)

TY  - JOUR
AU  - Radović, Nikola
AU  - Nikolić Jakoba, Nataša
AU  - Petrović, Nina
AU  - Milosavljević, Aleksandra
AU  - Brković, Božidar
AU  - Roganović, Jelena
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jcpe.12888
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7753
AB  - Aim: Recent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.
T2  - Journal of Clinical Periodontology
T1  - MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients
VL  - 45
IS  - 6
SP  - 663
EP  - 671
DO  - 10.1111/jcpe.12888
ER  - 
@article{
author = "Radović, Nikola and Nikolić Jakoba, Nataša and Petrović, Nina and Milosavljević, Aleksandra and Brković, Božidar and Roganović, Jelena",
year = "2018",
abstract = "Aim: Recent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively). Material and methodsmiR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically. ResultsThe upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender. ConclusionsmiR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.",
journal = "Journal of Clinical Periodontology",
title = "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients",
volume = "45",
number = "6",
pages = "663-671",
doi = "10.1111/jcpe.12888"
}
Radović, N., Nikolić Jakoba, N., Petrović, N., Milosavljević, A., Brković, B.,& Roganović, J.. (2018). MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. in Journal of Clinical Periodontology, 45(6), 663-671.
https://doi.org/10.1111/jcpe.12888
Radović N, Nikolić Jakoba N, Petrović N, Milosavljević A, Brković B, Roganović J. MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients. in Journal of Clinical Periodontology. 2018;45(6):663-671.
doi:10.1111/jcpe.12888 .
Radović, Nikola, Nikolić Jakoba, Nataša, Petrović, Nina, Milosavljević, Aleksandra, Brković, Božidar, Roganović, Jelena, "MicroRNA-146a and microRNA-155 as novel crevicular fluid biomarkers for periodontitis in non-diabetic and type 2 diabetic patients" in Journal of Clinical Periodontology, 45, no. 6 (2018):663-671,
https://doi.org/10.1111/jcpe.12888 . .
1
20
17
19

TIMP-3 mRNA expression levels positively correlates with levels of miR-21 in in situ BC and negatively in PR positive invasive BC

Petrović, Nina; Sami, Ahmad; Martinović, Jelena; Zarić, Marina; Nakashidze, Irina; Lukić, Silvana; Jovanović-Ćupić, Snežana P.

(2017)

TY  - JOUR
AU  - Petrović, Nina
AU  - Sami, Ahmad
AU  - Martinović, Jelena
AU  - Zarić, Marina
AU  - Nakashidze, Irina
AU  - Lukić, Silvana
AU  - Jovanović-Ćupić, Snežana P.
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1805
AB  - Background: Breast carcinomas (BC) belong to a heterogeneous group of malignant diseases. Correct categorization of BC based on molecular biomarkers has a very important role in deciding the proper course of therapy for each patient. It has been already shown that the decrease of TIMP metalloproteinase inhibitor 3 (TIMP-3) together with overexpression of microRNA-21 (miR-21) might be involved in the process of BC invasion. This is the first study that examined relationship among miR-21, TIMP-3 mRNA and TIPM-3 protein levels in BC groups formed according to invasiveness. Methods: In this study, we used 46 breast cancer samples. Estrogen and progesterone receptor (ER, PR) protein levels were evaluated by immunohistochemistry (IHC) method. TIMP-3 mRNA expression was examined by two-step real-time quantitative PCR (qRT-PCR). Western blot analysis was performed for 16 samples. Results: Statistically significant differences in TIMP-3 expression levels between invasive groups were discovered in ER positive (ER+) (p = 0.015), Her-2 negative (p = 0.026) subgroups, and patients without lymph-node metastasis (p = 0.039). Interestingly, significant positive correlation was detected between miR-21 and TIMP-3 mRNA levels (P LT 0.001, p = 0.949) in the group of in situ tumors. TIMP-3 mRNA expression levels highly negatively correlated with levels of miR-21 in PR + invasive BCs (p = 0.007, p = -0.641). TIMP-3 protein levels negatively correlated with miR-21 levels in pure invasive BCs. Conclusion: These data suggest that signaling pathways involved in formation and progression of BCs in groups formed according to invasiveness might be different. Our findings propose that TIMP-3 mRNA expression levels could be significant prognostic parameter, but within specific BC subtypes.
T2  - Pathology Research and Practice
T1  - TIMP-3 mRNA expression levels positively correlates with levels of miR-21 in in situ BC and negatively in PR positive invasive BC
VL  - 213
IS  - 10
SP  - 1264
EP  - 1270
DO  - 10.1016/j.prp.2017.08.012
ER  - 
@article{
author = "Petrović, Nina and Sami, Ahmad and Martinović, Jelena and Zarić, Marina and Nakashidze, Irina and Lukić, Silvana and Jovanović-Ćupić, Snežana P.",
year = "2017",
abstract = "Background: Breast carcinomas (BC) belong to a heterogeneous group of malignant diseases. Correct categorization of BC based on molecular biomarkers has a very important role in deciding the proper course of therapy for each patient. It has been already shown that the decrease of TIMP metalloproteinase inhibitor 3 (TIMP-3) together with overexpression of microRNA-21 (miR-21) might be involved in the process of BC invasion. This is the first study that examined relationship among miR-21, TIMP-3 mRNA and TIPM-3 protein levels in BC groups formed according to invasiveness. Methods: In this study, we used 46 breast cancer samples. Estrogen and progesterone receptor (ER, PR) protein levels were evaluated by immunohistochemistry (IHC) method. TIMP-3 mRNA expression was examined by two-step real-time quantitative PCR (qRT-PCR). Western blot analysis was performed for 16 samples. Results: Statistically significant differences in TIMP-3 expression levels between invasive groups were discovered in ER positive (ER+) (p = 0.015), Her-2 negative (p = 0.026) subgroups, and patients without lymph-node metastasis (p = 0.039). Interestingly, significant positive correlation was detected between miR-21 and TIMP-3 mRNA levels (P LT 0.001, p = 0.949) in the group of in situ tumors. TIMP-3 mRNA expression levels highly negatively correlated with levels of miR-21 in PR + invasive BCs (p = 0.007, p = -0.641). TIMP-3 protein levels negatively correlated with miR-21 levels in pure invasive BCs. Conclusion: These data suggest that signaling pathways involved in formation and progression of BCs in groups formed according to invasiveness might be different. Our findings propose that TIMP-3 mRNA expression levels could be significant prognostic parameter, but within specific BC subtypes.",
journal = "Pathology Research and Practice",
title = "TIMP-3 mRNA expression levels positively correlates with levels of miR-21 in in situ BC and negatively in PR positive invasive BC",
volume = "213",
number = "10",
pages = "1264-1270",
doi = "10.1016/j.prp.2017.08.012"
}
Petrović, N., Sami, A., Martinović, J., Zarić, M., Nakashidze, I., Lukić, S.,& Jovanović-Ćupić, S. P.. (2017). TIMP-3 mRNA expression levels positively correlates with levels of miR-21 in in situ BC and negatively in PR positive invasive BC. in Pathology Research and Practice, 213(10), 1264-1270.
https://doi.org/10.1016/j.prp.2017.08.012
Petrović N, Sami A, Martinović J, Zarić M, Nakashidze I, Lukić S, Jovanović-Ćupić SP. TIMP-3 mRNA expression levels positively correlates with levels of miR-21 in in situ BC and negatively in PR positive invasive BC. in Pathology Research and Practice. 2017;213(10):1264-1270.
doi:10.1016/j.prp.2017.08.012 .
Petrović, Nina, Sami, Ahmad, Martinović, Jelena, Zarić, Marina, Nakashidze, Irina, Lukić, Silvana, Jovanović-Ćupić, Snežana P., "TIMP-3 mRNA expression levels positively correlates with levels of miR-21 in in situ BC and negatively in PR positive invasive BC" in Pathology Research and Practice, 213, no. 10 (2017):1264-1270,
https://doi.org/10.1016/j.prp.2017.08.012 . .
4
4
4

MicroRNA in breast cancer: The association with BRCA1/2

Petrović, Nina; Davidović, Radoslav S.; Bajić, Vladan P.; Obradović, Milan M.; Isenović, Esma R.

(2017)

TY  - JOUR
AU  - Petrović, Nina
AU  - Davidović, Radoslav S.
AU  - Bajić, Vladan P.
AU  - Obradović, Milan M.
AU  - Isenović, Esma R.
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1614
AB  - Breast cancer (BC) is a heterogeneous disease in an urgent need for developing novel research, classification, and therapy approaches. Breast cancer 1 (BRCA1) and breast cancer 2 (BRCA2) proteins are well described tumor suppressors with great potential to be the subjects of different therapies. MicroRNAs (miRNAs) are genetic elements that might be used to solve the complex BC puzzle. BRCA1 was described to be the target of up to 100 miRNAs. BRCA1 may directly repress miR-155 activity. In addition, miR-15/107/182-mediated downregulation of BRCA1 interrupt DNA repair and may change the course of BC therapy. miR-146a and miR-146-5p silencing BRCA1 may trigger formation of triple-negative and basal-like sporadic BC cases. miR-182 might effect the therapy outcome. miR-21 targeted therapy might be useful for the treatment of BRCA2 mutation carriers. miR-342 overexpression and the absence of functional BRCA1 gene might cause synthetic lethality, which might be used as a base for future therapies. The present review discusses the latest data from studies that focus on the complex network of miRNAs and BRCA1/2 related BCs, which might be important for improving the therapy within the patients with triple-negative BC (TNBC) and basal-like BC, and for understanding the formation of TNBC.
T2  - Cancer Biomarkers
T1  - MicroRNA in breast cancer: The association with BRCA1/2
VL  - 19
IS  - 2
SP  - 119
EP  - 128
DO  - 10.3233/CBM-60319
ER  - 
@article{
author = "Petrović, Nina and Davidović, Radoslav S. and Bajić, Vladan P. and Obradović, Milan M. and Isenović, Esma R.",
year = "2017",
abstract = "Breast cancer (BC) is a heterogeneous disease in an urgent need for developing novel research, classification, and therapy approaches. Breast cancer 1 (BRCA1) and breast cancer 2 (BRCA2) proteins are well described tumor suppressors with great potential to be the subjects of different therapies. MicroRNAs (miRNAs) are genetic elements that might be used to solve the complex BC puzzle. BRCA1 was described to be the target of up to 100 miRNAs. BRCA1 may directly repress miR-155 activity. In addition, miR-15/107/182-mediated downregulation of BRCA1 interrupt DNA repair and may change the course of BC therapy. miR-146a and miR-146-5p silencing BRCA1 may trigger formation of triple-negative and basal-like sporadic BC cases. miR-182 might effect the therapy outcome. miR-21 targeted therapy might be useful for the treatment of BRCA2 mutation carriers. miR-342 overexpression and the absence of functional BRCA1 gene might cause synthetic lethality, which might be used as a base for future therapies. The present review discusses the latest data from studies that focus on the complex network of miRNAs and BRCA1/2 related BCs, which might be important for improving the therapy within the patients with triple-negative BC (TNBC) and basal-like BC, and for understanding the formation of TNBC.",
journal = "Cancer Biomarkers",
title = "MicroRNA in breast cancer: The association with BRCA1/2",
volume = "19",
number = "2",
pages = "119-128",
doi = "10.3233/CBM-60319"
}
Petrović, N., Davidović, R. S., Bajić, V. P., Obradović, M. M.,& Isenović, E. R.. (2017). MicroRNA in breast cancer: The association with BRCA1/2. in Cancer Biomarkers, 19(2), 119-128.
https://doi.org/10.3233/CBM-60319
Petrović N, Davidović RS, Bajić VP, Obradović MM, Isenović ER. MicroRNA in breast cancer: The association with BRCA1/2. in Cancer Biomarkers. 2017;19(2):119-128.
doi:10.3233/CBM-60319 .
Petrović, Nina, Davidović, Radoslav S., Bajić, Vladan P., Obradović, Milan M., Isenović, Esma R., "MicroRNA in breast cancer: The association with BRCA1/2" in Cancer Biomarkers, 19, no. 2 (2017):119-128,
https://doi.org/10.3233/CBM-60319 . .
33

A quality of life assessment and the correlation between generic and disease-specific questionnaires scores in outpatients with chronic liver disease-pilot study

Obradović, Milica; Gluvić, Zoran; Petrović, Nina; Obradović, Milan M.; Tomasevic, Ratko; Dugalic, Predrag; Isenović, Esma R.

(2017)

TY  - JOUR
AU  - Obradović, Milica
AU  - Gluvić, Zoran
AU  - Petrović, Nina
AU  - Obradović, Milan M.
AU  - Tomasevic, Ratko
AU  - Dugalic, Predrag
AU  - Isenović, Esma R.
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1700
AB  - Introduction. Chronic liver diseases (CLD) are an important cause of morbidity and mortality in general population. The aim of this study was to analyze potential differences between patients with CLD and healthy control group, and to estimate the severity of CLD by using simple questionnaires: general health questionnaire (GHQ-12) and chronic liver disease questionnaire (CLDQ). Methods. A cross-sectional pilot study was performed in Zemun Clinical Hospital during years 2014 and 2015. Sixty participants were divided into 4 groups (15 per group): chronic alcoholic hepatitis, other chronic hepatitis, liver cirrhosis, and healthy control group. Entire study population chose one of four offered answers of structured questionnaires GHQ-12 and CLDQ, based on which mean model of end-stage liver disease (MELD) and Child-Turcotte-Pugh (CTP) scores were calculated. Results. Mean GHQ12 and CLDQ scores were 10.5 and 5.21 +/- 1.11 respectively. Regarding certain CLDQ domain scores, a significant difference between alcoholic and non-alcoholic hepatitis groups in the worry domain was observed. Mean MELD score was 7.42 +/- 2.89 and did not differ between chronic hepatitis groups, while mean CTP score was 5.73 +/- 0.88. A statistically significant correlation was observed between GHQ12 and CLDQ scores (rho = -0.404, p LT 0.01), but not between subjective and objective scores. Conclusions. Mean GHQ12 and CLDQ scores pointed out to general psychological no-distress condition of the studied participants, as well as scarcely expressed CLD-specific complaints. Mean MELD and CTP scores indicated stable chronic liver diseases, with low three-month mortality rates in the cases of chronic hepatitis, as well as determination to Child A group in the case of liver cirrhosis.
T2  - Romanian Journal of Internal Medicine
T1  - A quality of life assessment and the correlation between generic and disease-specific questionnaires scores in outpatients with chronic liver disease-pilot study
VL  - 55
IS  - 3
SP  - 129
EP  - 137
DO  - 10.1515/rjim-2017-0014
ER  - 
@article{
author = "Obradović, Milica and Gluvić, Zoran and Petrović, Nina and Obradović, Milan M. and Tomasevic, Ratko and Dugalic, Predrag and Isenović, Esma R.",
year = "2017",
abstract = "Introduction. Chronic liver diseases (CLD) are an important cause of morbidity and mortality in general population. The aim of this study was to analyze potential differences between patients with CLD and healthy control group, and to estimate the severity of CLD by using simple questionnaires: general health questionnaire (GHQ-12) and chronic liver disease questionnaire (CLDQ). Methods. A cross-sectional pilot study was performed in Zemun Clinical Hospital during years 2014 and 2015. Sixty participants were divided into 4 groups (15 per group): chronic alcoholic hepatitis, other chronic hepatitis, liver cirrhosis, and healthy control group. Entire study population chose one of four offered answers of structured questionnaires GHQ-12 and CLDQ, based on which mean model of end-stage liver disease (MELD) and Child-Turcotte-Pugh (CTP) scores were calculated. Results. Mean GHQ12 and CLDQ scores were 10.5 and 5.21 +/- 1.11 respectively. Regarding certain CLDQ domain scores, a significant difference between alcoholic and non-alcoholic hepatitis groups in the worry domain was observed. Mean MELD score was 7.42 +/- 2.89 and did not differ between chronic hepatitis groups, while mean CTP score was 5.73 +/- 0.88. A statistically significant correlation was observed between GHQ12 and CLDQ scores (rho = -0.404, p LT 0.01), but not between subjective and objective scores. Conclusions. Mean GHQ12 and CLDQ scores pointed out to general psychological no-distress condition of the studied participants, as well as scarcely expressed CLD-specific complaints. Mean MELD and CTP scores indicated stable chronic liver diseases, with low three-month mortality rates in the cases of chronic hepatitis, as well as determination to Child A group in the case of liver cirrhosis.",
journal = "Romanian Journal of Internal Medicine",
title = "A quality of life assessment and the correlation between generic and disease-specific questionnaires scores in outpatients with chronic liver disease-pilot study",
volume = "55",
number = "3",
pages = "129-137",
doi = "10.1515/rjim-2017-0014"
}
Obradović, M., Gluvić, Z., Petrović, N., Obradović, M. M., Tomasevic, R., Dugalic, P.,& Isenović, E. R.. (2017). A quality of life assessment and the correlation between generic and disease-specific questionnaires scores in outpatients with chronic liver disease-pilot study. in Romanian Journal of Internal Medicine, 55(3), 129-137.
https://doi.org/10.1515/rjim-2017-0014
Obradović M, Gluvić Z, Petrović N, Obradović MM, Tomasevic R, Dugalic P, Isenović ER. A quality of life assessment and the correlation between generic and disease-specific questionnaires scores in outpatients with chronic liver disease-pilot study. in Romanian Journal of Internal Medicine. 2017;55(3):129-137.
doi:10.1515/rjim-2017-0014 .
Obradović, Milica, Gluvić, Zoran, Petrović, Nina, Obradović, Milan M., Tomasevic, Ratko, Dugalic, Predrag, Isenović, Esma R., "A quality of life assessment and the correlation between generic and disease-specific questionnaires scores in outpatients with chronic liver disease-pilot study" in Romanian Journal of Internal Medicine, 55, no. 3 (2017):129-137,
https://doi.org/10.1515/rjim-2017-0014 . .
2
1
1

Levels of MicroRNA Heterogeneity in Cancer Biology

Petrović, Nina; Ergun, Sercan; Isenović, Esma R.

(2017)

TY  - JOUR
AU  - Petrović, Nina
AU  - Ergun, Sercan
AU  - Isenović, Esma R.
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1736
AB  - MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression, involved in the silencing of messenger RNA (mRNA) translation. The importance of miRNA signatures in disease screening, prognosis, and progression of different tumor types and subtypes is increasing. miRNA expression levels change depending on numerous factors. In this review, we are describing the circumstances under which miRNA levels can change, these are named levels of heterogeneity of miRNAs. miRNAs can have oncogenic, tumor suppressive, or both roles depending on tumor type and target mRNA whose translation they silence. The expression levels of a single miRNA may vary across different cancer types and subtypes, indicating that a miRNA signature may be tissue specific. miRNA levels of expression also vary during disease formation and propagation, indicating the presence of a time profile for their expression. The complexity of the miRNA-mRNA interference network mirrors different genetic and epigenetic changes that influence miRNA and mRNA availability to each other, and hence, their binding ability. The potential role of miRNAs as biomarkers is two-fold; first, for monitoring of the phases of cancer pathogenesis, and second, to characterize the particular type/subtype of cancer. It is important that a particular miRNA should be characterized by examining as many types and subtypes of cancers as are available, as well as being extracted from different types of samples, in order to obtain a complete picture of its behavior and importance in the disease pathology.
T2  - Molecular Diagnosis and Therapy
T1  - Levels of MicroRNA Heterogeneity in Cancer Biology
VL  - 21
IS  - 5
SP  - 511
EP  - 523
DO  - 10.1007/s40291-017-0285-9
ER  - 
@article{
author = "Petrović, Nina and Ergun, Sercan and Isenović, Esma R.",
year = "2017",
abstract = "MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression, involved in the silencing of messenger RNA (mRNA) translation. The importance of miRNA signatures in disease screening, prognosis, and progression of different tumor types and subtypes is increasing. miRNA expression levels change depending on numerous factors. In this review, we are describing the circumstances under which miRNA levels can change, these are named levels of heterogeneity of miRNAs. miRNAs can have oncogenic, tumor suppressive, or both roles depending on tumor type and target mRNA whose translation they silence. The expression levels of a single miRNA may vary across different cancer types and subtypes, indicating that a miRNA signature may be tissue specific. miRNA levels of expression also vary during disease formation and propagation, indicating the presence of a time profile for their expression. The complexity of the miRNA-mRNA interference network mirrors different genetic and epigenetic changes that influence miRNA and mRNA availability to each other, and hence, their binding ability. The potential role of miRNAs as biomarkers is two-fold; first, for monitoring of the phases of cancer pathogenesis, and second, to characterize the particular type/subtype of cancer. It is important that a particular miRNA should be characterized by examining as many types and subtypes of cancers as are available, as well as being extracted from different types of samples, in order to obtain a complete picture of its behavior and importance in the disease pathology.",
journal = "Molecular Diagnosis and Therapy",
title = "Levels of MicroRNA Heterogeneity in Cancer Biology",
volume = "21",
number = "5",
pages = "511-523",
doi = "10.1007/s40291-017-0285-9"
}
Petrović, N., Ergun, S.,& Isenović, E. R.. (2017). Levels of MicroRNA Heterogeneity in Cancer Biology. in Molecular Diagnosis and Therapy, 21(5), 511-523.
https://doi.org/10.1007/s40291-017-0285-9
Petrović N, Ergun S, Isenović ER. Levels of MicroRNA Heterogeneity in Cancer Biology. in Molecular Diagnosis and Therapy. 2017;21(5):511-523.
doi:10.1007/s40291-017-0285-9 .
Petrović, Nina, Ergun, Sercan, Isenović, Esma R., "Levels of MicroRNA Heterogeneity in Cancer Biology" in Molecular Diagnosis and Therapy, 21, no. 5 (2017):511-523,
https://doi.org/10.1007/s40291-017-0285-9 . .
29
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27

Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels

Petrović, Nina; Davidović, Radoslav S.; Jovanović-Ćupić, Snežana P.; Krajnović, Milena M.; Lukić, Silvana; Petrović, Milan; Roganović, Jelena

(2016)

TY  - JOUR
AU  - Petrović, Nina
AU  - Davidović, Radoslav S.
AU  - Jovanović-Ćupić, Snežana P.
AU  - Krajnović, Milena M.
AU  - Lukić, Silvana
AU  - Petrović, Milan
AU  - Roganović, Jelena
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1308
AB  - Breast cancer (BC) is a heterogeneous group of diseases that still represents a major cause of death in the female population. MicroRNAs (miRNAs, miRs), such as miR-221 and miR-222, have been shown to be involved in BC pathology by acting via its target genes such as tissue inhibitor of metalloproteinase 3 (TIMP3). The main goals of this study were to find differences in miR-221/222 levels of expression in BC groups based on invasiveness, and to investigate the association with estrogen receptor (ER), TIMP3 messenger RNA (mRNA) levels, and clinicopathological characteristics of patients and tumors. In this study, we measured levels of miR-221/222 in 63 breast tissue samples by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) using TaqMan(A (R)) technology and immunohistochemistry. miR-221/222 levels varied significantly across groups based on invasiveness (P LT 0.001). In in situ tumors, miR-221 and miR-222 were negatively associated with ER (P = 0.001, r = -0.714, and P = 0.013, r = -0.585, respectively). In invasive breast carcinomas associated with non-invasive tumors, miR-222 was inversely associated with ER (P = 0.039, r = -0.620). Pure invasive BCs showed a positive correlation of miR-221 and miR-222 with TIMP3 mRNA levels (P = 0.008, r = 0.508, and P = 0.010, r = 0.497, respectively). An increase in miR-221/222 might be an important event for in situ carcinoma formation, and miR-221/222 may be important molecules that highlight potential differences between invasive breast carcinomas associated with non-invasive and pure invasive BCs.
T2  - Molecular Diagnosis and Therapy
T1  - Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels
VL  - 20
IS  - 6
SP  - 603
EP  - 615
DO  - 10.1007/s40291-016-0230-3
ER  - 
@article{
author = "Petrović, Nina and Davidović, Radoslav S. and Jovanović-Ćupić, Snežana P. and Krajnović, Milena M. and Lukić, Silvana and Petrović, Milan and Roganović, Jelena",
year = "2016",
abstract = "Breast cancer (BC) is a heterogeneous group of diseases that still represents a major cause of death in the female population. MicroRNAs (miRNAs, miRs), such as miR-221 and miR-222, have been shown to be involved in BC pathology by acting via its target genes such as tissue inhibitor of metalloproteinase 3 (TIMP3). The main goals of this study were to find differences in miR-221/222 levels of expression in BC groups based on invasiveness, and to investigate the association with estrogen receptor (ER), TIMP3 messenger RNA (mRNA) levels, and clinicopathological characteristics of patients and tumors. In this study, we measured levels of miR-221/222 in 63 breast tissue samples by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) using TaqMan(A (R)) technology and immunohistochemistry. miR-221/222 levels varied significantly across groups based on invasiveness (P LT 0.001). In in situ tumors, miR-221 and miR-222 were negatively associated with ER (P = 0.001, r = -0.714, and P = 0.013, r = -0.585, respectively). In invasive breast carcinomas associated with non-invasive tumors, miR-222 was inversely associated with ER (P = 0.039, r = -0.620). Pure invasive BCs showed a positive correlation of miR-221 and miR-222 with TIMP3 mRNA levels (P = 0.008, r = 0.508, and P = 0.010, r = 0.497, respectively). An increase in miR-221/222 might be an important event for in situ carcinoma formation, and miR-221/222 may be important molecules that highlight potential differences between invasive breast carcinomas associated with non-invasive and pure invasive BCs.",
journal = "Molecular Diagnosis and Therapy",
title = "Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels",
volume = "20",
number = "6",
pages = "603-615",
doi = "10.1007/s40291-016-0230-3"
}
Petrović, N., Davidović, R. S., Jovanović-Ćupić, S. P., Krajnović, M. M., Lukić, S., Petrović, M.,& Roganović, J.. (2016). Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels. in Molecular Diagnosis and Therapy, 20(6), 603-615.
https://doi.org/10.1007/s40291-016-0230-3
Petrović N, Davidović RS, Jovanović-Ćupić SP, Krajnović MM, Lukić S, Petrović M, Roganović J. Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels. in Molecular Diagnosis and Therapy. 2016;20(6):603-615.
doi:10.1007/s40291-016-0230-3 .
Petrović, Nina, Davidović, Radoslav S., Jovanović-Ćupić, Snežana P., Krajnović, Milena M., Lukić, Silvana, Petrović, Milan, Roganović, Jelena, "Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels" in Molecular Diagnosis and Therapy, 20, no. 6 (2016):603-615,
https://doi.org/10.1007/s40291-016-0230-3 . .
1
12
10
11

miR-155 expression level changes might be associated with initial phases of breast cancer pathogenesis and lymph-node metastasis

Petrović, Nina; Kolaković, Ana; Stanković, Aleksandra; Lukić, Silvana; Sami, Ahmad; Živković, Maja; Mandušić, Vesna

(2016)

TY  - JOUR
AU  - Petrović, Nina
AU  - Kolaković, Ana
AU  - Stanković, Aleksandra
AU  - Lukić, Silvana
AU  - Sami, Ahmad
AU  - Živković, Maja
AU  - Mandušić, Vesna
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1023
AB  - BACKGROUND: Breast carcinoma is heterogeneous disease. Understanding the process of invasion and metastasis and the selection of the therapy for patients with breast carcinomas still remains difficult. MicroRNAs are powerful gene expression regulators. Because of inconsistent findings, we have analyzed potential difference in miR-155 levels in three breast cancer groups. OBJECTIVES: Our goals were to examine miR-155 expression levels in normal tissue, non-invasive and invasive breast carcinomas, and their association with standard clinical and pathological parameters and oncomiR-21, and to investigate the ability of miR-155 to separate invasive breast carcinomas with non-invasive component from pure invasive. METHODS: In the group of 40 breast tissue samples, relative expression levels of miR-155 were examined with stem-loop quantitative real-time PCR using TaqMan technology. RESULTS: The significant difference among four examined groups of the breast tissue was detected (p = 0.001). In the group of pure invasive tumors, patients with positive nodal status had significantly higher miR-155 levels (p = 0.046). CONCLUSION: Our results suggest that miR-155 might be involved in breast cancer pathogenesis and in tumor spreading to the lymph nodes, and that it might be used as biomarker for additional stratification of patients with invasive breast carcinomas with non-invasive component.
T2  - Cancer Biomarkers
T1  - miR-155 expression level changes might be associated with initial phases of breast cancer pathogenesis and lymph-node metastasis
VL  - 16
IS  - 3
SP  - 385
EP  - 394
DO  - 10.3233/CBM-160577
ER  - 
@article{
author = "Petrović, Nina and Kolaković, Ana and Stanković, Aleksandra and Lukić, Silvana and Sami, Ahmad and Živković, Maja and Mandušić, Vesna",
year = "2016",
abstract = "BACKGROUND: Breast carcinoma is heterogeneous disease. Understanding the process of invasion and metastasis and the selection of the therapy for patients with breast carcinomas still remains difficult. MicroRNAs are powerful gene expression regulators. Because of inconsistent findings, we have analyzed potential difference in miR-155 levels in three breast cancer groups. OBJECTIVES: Our goals were to examine miR-155 expression levels in normal tissue, non-invasive and invasive breast carcinomas, and their association with standard clinical and pathological parameters and oncomiR-21, and to investigate the ability of miR-155 to separate invasive breast carcinomas with non-invasive component from pure invasive. METHODS: In the group of 40 breast tissue samples, relative expression levels of miR-155 were examined with stem-loop quantitative real-time PCR using TaqMan technology. RESULTS: The significant difference among four examined groups of the breast tissue was detected (p = 0.001). In the group of pure invasive tumors, patients with positive nodal status had significantly higher miR-155 levels (p = 0.046). CONCLUSION: Our results suggest that miR-155 might be involved in breast cancer pathogenesis and in tumor spreading to the lymph nodes, and that it might be used as biomarker for additional stratification of patients with invasive breast carcinomas with non-invasive component.",
journal = "Cancer Biomarkers",
title = "miR-155 expression level changes might be associated with initial phases of breast cancer pathogenesis and lymph-node metastasis",
volume = "16",
number = "3",
pages = "385-394",
doi = "10.3233/CBM-160577"
}
Petrović, N., Kolaković, A., Stanković, A., Lukić, S., Sami, A., Živković, M.,& Mandušić, V.. (2016). miR-155 expression level changes might be associated with initial phases of breast cancer pathogenesis and lymph-node metastasis. in Cancer Biomarkers, 16(3), 385-394.
https://doi.org/10.3233/CBM-160577
Petrović N, Kolaković A, Stanković A, Lukić S, Sami A, Živković M, Mandušić V. miR-155 expression level changes might be associated with initial phases of breast cancer pathogenesis and lymph-node metastasis. in Cancer Biomarkers. 2016;16(3):385-394.
doi:10.3233/CBM-160577 .
Petrović, Nina, Kolaković, Ana, Stanković, Aleksandra, Lukić, Silvana, Sami, Ahmad, Živković, Maja, Mandušić, Vesna, "miR-155 expression level changes might be associated with initial phases of breast cancer pathogenesis and lymph-node metastasis" in Cancer Biomarkers, 16, no. 3 (2016):385-394,
https://doi.org/10.3233/CBM-160577 . .
1
17
17
16

miR-21 Might be Involved in Breast Cancer Promotion and Invasion Rather than in Initial Events of Breast Cancer Development

Petrović, Nina

(Springer, 2016)

TY  - JOUR
AU  - Petrović, Nina
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/985
AB  - Breast cancer (BC) is a heterogeneous disease that develops into a large number of varied phenotypes. One of the features used in its classification and therapy selection is invasiveness. MicroRNA-21 (miR-21) is considered to be an important element of BC invasiveness, and miR-21 levels are frequently increased in different tumor types compared with normal tissue, including the breast. Experimental and literature research has highlighted that miR-21 was always significantly elevated in every study that included invasive breast carcinomas compared with healthy breast tissue. The main goal of this research was to specify the predominant role of miR-21 in the different phases of BC pathogenesis, i.e. whether it was involved in the early (initiation), later (promotion), or late (propagation, progression) phases. Our second goal was to explain the roles of miR-21 targets in BC by an in silico approach and literature review, and to associate the importance of miR-21 with particular phases of BC pathogenesis through the action of its target genes. Analysis has shown that changes in miR-21 levels might be important for the later and/or late phases of breast cancerogenesis rather than for the initial early phases. Targets of miR-21 (TIMP3, PDCD4, PTEN, TPM1 and RECK) are also primarily involved in BC promotion and progression, especially invasion, angiogenesis and metastasis. miR-21 expression levels could perhaps be used in conjunction with the standard diagnostic parameters as an indicator of BC presence, and to indicate a phenotype likely to show early invasion/metastasis detection and poor prognosis.
PB  - Springer
T2  - Molecular Diagnosis and Therapy
T1  - miR-21 Might be Involved in Breast Cancer Promotion and Invasion Rather than in Initial Events of Breast Cancer Development
VL  - 20
IS  - 2
SP  - 97
EP  - 110
DO  - 10.1007/s40291-016-0186-3
ER  - 
@article{
author = "Petrović, Nina",
year = "2016",
abstract = "Breast cancer (BC) is a heterogeneous disease that develops into a large number of varied phenotypes. One of the features used in its classification and therapy selection is invasiveness. MicroRNA-21 (miR-21) is considered to be an important element of BC invasiveness, and miR-21 levels are frequently increased in different tumor types compared with normal tissue, including the breast. Experimental and literature research has highlighted that miR-21 was always significantly elevated in every study that included invasive breast carcinomas compared with healthy breast tissue. The main goal of this research was to specify the predominant role of miR-21 in the different phases of BC pathogenesis, i.e. whether it was involved in the early (initiation), later (promotion), or late (propagation, progression) phases. Our second goal was to explain the roles of miR-21 targets in BC by an in silico approach and literature review, and to associate the importance of miR-21 with particular phases of BC pathogenesis through the action of its target genes. Analysis has shown that changes in miR-21 levels might be important for the later and/or late phases of breast cancerogenesis rather than for the initial early phases. Targets of miR-21 (TIMP3, PDCD4, PTEN, TPM1 and RECK) are also primarily involved in BC promotion and progression, especially invasion, angiogenesis and metastasis. miR-21 expression levels could perhaps be used in conjunction with the standard diagnostic parameters as an indicator of BC presence, and to indicate a phenotype likely to show early invasion/metastasis detection and poor prognosis.",
publisher = "Springer",
journal = "Molecular Diagnosis and Therapy",
title = "miR-21 Might be Involved in Breast Cancer Promotion and Invasion Rather than in Initial Events of Breast Cancer Development",
volume = "20",
number = "2",
pages = "97-110",
doi = "10.1007/s40291-016-0186-3"
}
Petrović, N.. (2016). miR-21 Might be Involved in Breast Cancer Promotion and Invasion Rather than in Initial Events of Breast Cancer Development. in Molecular Diagnosis and Therapy
Springer., 20(2), 97-110.
https://doi.org/10.1007/s40291-016-0186-3
Petrović N. miR-21 Might be Involved in Breast Cancer Promotion and Invasion Rather than in Initial Events of Breast Cancer Development. in Molecular Diagnosis and Therapy. 2016;20(2):97-110.
doi:10.1007/s40291-016-0186-3 .
Petrović, Nina, "miR-21 Might be Involved in Breast Cancer Promotion and Invasion Rather than in Initial Events of Breast Cancer Development" in Molecular Diagnosis and Therapy, 20, no. 2 (2016):97-110,
https://doi.org/10.1007/s40291-016-0186-3 . .
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33

The influence of host factors and sequence variability of the p7 region on the response to pegylated interferon/ribavirin therapy for chronic hepatitis C genotype 1b in patients from Serbia

Jovanović-Ćupić, Snežana P.; Glišić, Sanja; Stanojevic, Maja; Nozic, Darko; Petrović, Nina; Mandušić, Vesna; Krajnović, Milena M.

(Springer, 2016)

TY  - JOUR
AU  - Jovanović-Ćupić, Snežana P.
AU  - Glišić, Sanja
AU  - Stanojevic, Maja
AU  - Nozic, Darko
AU  - Petrović, Nina
AU  - Mandušić, Vesna
AU  - Krajnović, Milena M.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1032
AB  - The goal of this study was to identify host and viral factors affecting the response to pegylated interferon/ribavirin (PEG-IFN/RBV) treatment in patients with chronic hepatitis C genotype 1b. Baseline characteristics of the patients and sequences within the p7 region were analyzed in pre-treatment serum samples from 53 individuals with chronic hepatitis C genotype 1b and related to the outcome of therapy. We found a significant correlation between age and response to therapy (p LT 0.001). Furthermore, the pre-treatment viral load was closely associated with the stage of liver fibrosis (p LT 0.001). The presence of fewer than 4 mutations and age above 40 were significantly associated with non-response (NR) (p LT 0.001). Our findings may be useful for estimating the likelihood of achieving a sustained virologic response (SVR) in patients who are chronically infected with hepatitis C virus genotype 1b.
PB  - Springer
T2  - Archives of Virology
T1  - The influence of host factors and sequence variability of the p7 region on the response to pegylated interferon/ribavirin therapy for chronic hepatitis C genotype 1b in patients from Serbia
VL  - 161
IS  - 5
SP  - 1189
EP  - 1198
DO  - 10.1007/s00705-016-2777-z
ER  - 
@article{
author = "Jovanović-Ćupić, Snežana P. and Glišić, Sanja and Stanojevic, Maja and Nozic, Darko and Petrović, Nina and Mandušić, Vesna and Krajnović, Milena M.",
year = "2016",
abstract = "The goal of this study was to identify host and viral factors affecting the response to pegylated interferon/ribavirin (PEG-IFN/RBV) treatment in patients with chronic hepatitis C genotype 1b. Baseline characteristics of the patients and sequences within the p7 region were analyzed in pre-treatment serum samples from 53 individuals with chronic hepatitis C genotype 1b and related to the outcome of therapy. We found a significant correlation between age and response to therapy (p LT 0.001). Furthermore, the pre-treatment viral load was closely associated with the stage of liver fibrosis (p LT 0.001). The presence of fewer than 4 mutations and age above 40 were significantly associated with non-response (NR) (p LT 0.001). Our findings may be useful for estimating the likelihood of achieving a sustained virologic response (SVR) in patients who are chronically infected with hepatitis C virus genotype 1b.",
publisher = "Springer",
journal = "Archives of Virology",
title = "The influence of host factors and sequence variability of the p7 region on the response to pegylated interferon/ribavirin therapy for chronic hepatitis C genotype 1b in patients from Serbia",
volume = "161",
number = "5",
pages = "1189-1198",
doi = "10.1007/s00705-016-2777-z"
}
Jovanović-Ćupić, S. P., Glišić, S., Stanojevic, M., Nozic, D., Petrović, N., Mandušić, V.,& Krajnović, M. M.. (2016). The influence of host factors and sequence variability of the p7 region on the response to pegylated interferon/ribavirin therapy for chronic hepatitis C genotype 1b in patients from Serbia. in Archives of Virology
Springer., 161(5), 1189-1198.
https://doi.org/10.1007/s00705-016-2777-z
Jovanović-Ćupić SP, Glišić S, Stanojevic M, Nozic D, Petrović N, Mandušić V, Krajnović MM. The influence of host factors and sequence variability of the p7 region on the response to pegylated interferon/ribavirin therapy for chronic hepatitis C genotype 1b in patients from Serbia. in Archives of Virology. 2016;161(5):1189-1198.
doi:10.1007/s00705-016-2777-z .
Jovanović-Ćupić, Snežana P., Glišić, Sanja, Stanojevic, Maja, Nozic, Darko, Petrović, Nina, Mandušić, Vesna, Krajnović, Milena M., "The influence of host factors and sequence variability of the p7 region on the response to pegylated interferon/ribavirin therapy for chronic hepatitis C genotype 1b in patients from Serbia" in Archives of Virology, 161, no. 5 (2016):1189-1198,
https://doi.org/10.1007/s00705-016-2777-z . .
1
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1

Micro Rna-21 Expression Levels in Invasive Breast Carcinoma with a Non-Invasive Component

Petrović, Nina; Jovanović-Ćupić, Snežana P.; Brajušković, Goran; Lukić, Silvana; Roganović, Jelena; Krajnović, Milena M.; Mandušić, Vesna

(2015)

TY  - JOUR
AU  - Petrović, Nina
AU  - Jovanović-Ćupić, Snežana P.
AU  - Brajušković, Goran
AU  - Lukić, Silvana
AU  - Roganović, Jelena
AU  - Krajnović, Milena M.
AU  - Mandušić, Vesna
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/862
AB  - Invasive ductal carcinomas with a non-invasive component (IDC-DCIS) are classified as a group of invasive breast carcinomas, together with pure invasive ductal carcinomas of the breast (IDC). MicroRNA-21 (miR-21) has been characterized as a factor of breast cancer invasiveness, however the difference in miR-21 expression levels between IDC-DCIS and pure IDC tumors and the correlations with standard diagnostic and prognostic parameters inside the IDC-DCIS group are unknown. Our aim was to determine if miR-21 had the ability to distinguish these two invasive breast cancer groups. Levels of miR-21 expression were measured by a stem-loop quantitative Real-Time PCR (RT-qPCR) method. Expression levels of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her-2) and proliferative index Ki-67 were evaluated by immunohistochemistry. IDC-DCIS tumors had significantly lower levels of miR-21 expression in grade 2 (P=0.003, Mann-Whitney U test), ER positive (P=0.025, Mann-Whitney U test) and PR positive tumors (P=0.024, Mann-Whitney U test) than pure IDCs. miR-21 levels showed a different pattern of expression in IDC-DCIS compared to IDC tumors, which is based on the difference in miR-21 expression between Her-2 negative and Her-2 positive IDC-DCIS tumors (P=0.030, Mann-Whitney U test) and high negative correlation of miR-21 levels with PR levels (rho=-0.886, P=0.006, Spearman correlation). According to our results, IDC-DCIS breast carcinomas act in a different manner in pure IDC tumors with regard to the relations between miR-21 expression levels and the standard diagnostic and prognostic parameters, such as Her-2 status, ER and PR status and protein levels.
T2  - Archives of biological sciences
T1  - Micro Rna-21 Expression Levels in Invasive Breast Carcinoma with a Non-Invasive Component
VL  - 67
IS  - 4
SP  - 1285
EP  - 1295
DO  - 10.2298/ABS150327105P
ER  - 
@article{
author = "Petrović, Nina and Jovanović-Ćupić, Snežana P. and Brajušković, Goran and Lukić, Silvana and Roganović, Jelena and Krajnović, Milena M. and Mandušić, Vesna",
year = "2015",
abstract = "Invasive ductal carcinomas with a non-invasive component (IDC-DCIS) are classified as a group of invasive breast carcinomas, together with pure invasive ductal carcinomas of the breast (IDC). MicroRNA-21 (miR-21) has been characterized as a factor of breast cancer invasiveness, however the difference in miR-21 expression levels between IDC-DCIS and pure IDC tumors and the correlations with standard diagnostic and prognostic parameters inside the IDC-DCIS group are unknown. Our aim was to determine if miR-21 had the ability to distinguish these two invasive breast cancer groups. Levels of miR-21 expression were measured by a stem-loop quantitative Real-Time PCR (RT-qPCR) method. Expression levels of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her-2) and proliferative index Ki-67 were evaluated by immunohistochemistry. IDC-DCIS tumors had significantly lower levels of miR-21 expression in grade 2 (P=0.003, Mann-Whitney U test), ER positive (P=0.025, Mann-Whitney U test) and PR positive tumors (P=0.024, Mann-Whitney U test) than pure IDCs. miR-21 levels showed a different pattern of expression in IDC-DCIS compared to IDC tumors, which is based on the difference in miR-21 expression between Her-2 negative and Her-2 positive IDC-DCIS tumors (P=0.030, Mann-Whitney U test) and high negative correlation of miR-21 levels with PR levels (rho=-0.886, P=0.006, Spearman correlation). According to our results, IDC-DCIS breast carcinomas act in a different manner in pure IDC tumors with regard to the relations between miR-21 expression levels and the standard diagnostic and prognostic parameters, such as Her-2 status, ER and PR status and protein levels.",
journal = "Archives of biological sciences",
title = "Micro Rna-21 Expression Levels in Invasive Breast Carcinoma with a Non-Invasive Component",
volume = "67",
number = "4",
pages = "1285-1295",
doi = "10.2298/ABS150327105P"
}
Petrović, N., Jovanović-Ćupić, S. P., Brajušković, G., Lukić, S., Roganović, J., Krajnović, M. M.,& Mandušić, V.. (2015). Micro Rna-21 Expression Levels in Invasive Breast Carcinoma with a Non-Invasive Component. in Archives of biological sciences, 67(4), 1285-1295.
https://doi.org/10.2298/ABS150327105P
Petrović N, Jovanović-Ćupić SP, Brajušković G, Lukić S, Roganović J, Krajnović MM, Mandušić V. Micro Rna-21 Expression Levels in Invasive Breast Carcinoma with a Non-Invasive Component. in Archives of biological sciences. 2015;67(4):1285-1295.
doi:10.2298/ABS150327105P .
Petrović, Nina, Jovanović-Ćupić, Snežana P., Brajušković, Goran, Lukić, Silvana, Roganović, Jelena, Krajnović, Milena M., Mandušić, Vesna, "Micro Rna-21 Expression Levels in Invasive Breast Carcinoma with a Non-Invasive Component" in Archives of biological sciences, 67, no. 4 (2015):1285-1295,
https://doi.org/10.2298/ABS150327105P . .
2
2
2

Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia

Petrović, Nina; Mandušić, Vesna; Dimitrijević, Bogomir B.; Roganović, Jelena; Lukić, Silvana; Todorović, Lidija; Stanojević, Boban

(2014)

TY  - JOUR
AU  - Petrović, Nina
AU  - Mandušić, Vesna
AU  - Dimitrijević, Bogomir B.
AU  - Roganović, Jelena
AU  - Lukić, Silvana
AU  - Todorović, Lidija
AU  - Stanojević, Boban
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6070
AB  - MicroRNAs play essential role in breast carcinoma progression and invasion. Our principal goals were to assess clinicopathological and prognostic correlations of microRNA-21 (miR-21) expression levels in a group of 39 Serbian breast cancer patients with invasive lobular (ILC), ductal (IDC), or mixed (ILC-IDC) breast carcinomas and in order to discover the role of miR-21 in potential novel form of stratification of the patients with different estrogen receptor (ER) and progesterone receptor (PR) status. MiR-21 expression levels were measured by stem-loop real-time RT-PCR using TaqMan technology. ER, PR, human epidermal growth factor 2 receptor (Her-2), and proliferative index (Ki-67) were evaluated by immunohistochemistry. MiR-21 levels do not vary among ILC, IDC, and ILC-IDC subgroups. MiR-21 expression levels varied significantly in the age, tumor size, Ki-67, and different grade (p = 0.030, p = 0.036, p = 0.027 and p = 0.032, respectively) subgroups. ER? and PR? showed higher miR-21 levels than their negative receptor status paired groups ER-and PR-with p = 0.012 and p = 0.018, respectively. MiR-21 positively correlated with ER and PR status (p = 0.018, rho = 0.379 and p = 0.034, rho = 0.345, respectively). Our findings suggest that miR-21 emulates transitional form of expression and that the levels of expression might be useful for stratification of the patients with different receptor status with the purpose to seek for new therapy approaches especially for the patients with the lack of response to conventional endocrine therapy.
T2  - Medical Oncology
T1  - Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia
VL  - 31
IS  - 6
DO  - 10.1007/s12032-014-0977-5
ER  - 
@article{
author = "Petrović, Nina and Mandušić, Vesna and Dimitrijević, Bogomir B. and Roganović, Jelena and Lukić, Silvana and Todorović, Lidija and Stanojević, Boban",
year = "2014",
abstract = "MicroRNAs play essential role in breast carcinoma progression and invasion. Our principal goals were to assess clinicopathological and prognostic correlations of microRNA-21 (miR-21) expression levels in a group of 39 Serbian breast cancer patients with invasive lobular (ILC), ductal (IDC), or mixed (ILC-IDC) breast carcinomas and in order to discover the role of miR-21 in potential novel form of stratification of the patients with different estrogen receptor (ER) and progesterone receptor (PR) status. MiR-21 expression levels were measured by stem-loop real-time RT-PCR using TaqMan technology. ER, PR, human epidermal growth factor 2 receptor (Her-2), and proliferative index (Ki-67) were evaluated by immunohistochemistry. MiR-21 levels do not vary among ILC, IDC, and ILC-IDC subgroups. MiR-21 expression levels varied significantly in the age, tumor size, Ki-67, and different grade (p = 0.030, p = 0.036, p = 0.027 and p = 0.032, respectively) subgroups. ER? and PR? showed higher miR-21 levels than their negative receptor status paired groups ER-and PR-with p = 0.012 and p = 0.018, respectively. MiR-21 positively correlated with ER and PR status (p = 0.018, rho = 0.379 and p = 0.034, rho = 0.345, respectively). Our findings suggest that miR-21 emulates transitional form of expression and that the levels of expression might be useful for stratification of the patients with different receptor status with the purpose to seek for new therapy approaches especially for the patients with the lack of response to conventional endocrine therapy.",
journal = "Medical Oncology",
title = "Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia",
volume = "31",
number = "6",
doi = "10.1007/s12032-014-0977-5"
}
Petrović, N., Mandušić, V., Dimitrijević, B. B., Roganović, J., Lukić, S., Todorović, L.,& Stanojević, B.. (2014). Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia. in Medical Oncology, 31(6).
https://doi.org/10.1007/s12032-014-0977-5
Petrović N, Mandušić V, Dimitrijević BB, Roganović J, Lukić S, Todorović L, Stanojević B. Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia. in Medical Oncology. 2014;31(6).
doi:10.1007/s12032-014-0977-5 .
Petrović, Nina, Mandušić, Vesna, Dimitrijević, Bogomir B., Roganović, Jelena, Lukić, Silvana, Todorović, Lidija, Stanojević, Boban, "Higher miR-21 expression in invasive breast carcinomas is associated with positive estrogen and progesterone receptor status in patients from Serbia" in Medical Oncology, 31, no. 6 (2014),
https://doi.org/10.1007/s12032-014-0977-5 . .
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The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion

Petrović, Nina; Mandušić, Vesna; Stanojević, Boban; Lukić, Silvana; Todorović, Lidija; Roganović, Jelena; Dimitrijević, Bogomir B.

(2014)

TY  - JOUR
AU  - Petrović, Nina
AU  - Mandušić, Vesna
AU  - Stanojević, Boban
AU  - Lukić, Silvana
AU  - Todorović, Lidija
AU  - Roganović, Jelena
AU  - Dimitrijević, Bogomir B.
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6041
AB  - MicroRNA-21 (miR-21) overexpression is characteristic for various types of tumors, but it is still unknown whether its expression levels differ between invasive and non-invasive breast carcinomas. The main goal of the study was to determine the difference in miR-21 expression among normal tissue, non-invasive, invasive with non-invasive component, and pure invasive breast cancer samples, to explain its potential role and significance in breast cancer invasiveness. The second goal was to propose miR-21 as molecular marker of breast cancer invasiveness and potential target for future anti-miR therapies for the prevention of invasion and metastasis. In order to reveal the role of miR-21 in breast cancer invasiveness, we measured miR-21 expression levels in 44 breast cancer and four normal samples by stem-loop real-time RT-PCR using TaqMan technology. Relative expression levels of miR-21 were significantly higher in invasive than in other groups (P = 0.002) and significantly higher in invasive compared with invasive with non-invasive component group in histological (P = 0.043) and nuclear grade 2 (P = 0.036), estrogen-receptor-positive (ER+) (P = 0.006), progesterone-receptor-positive (PR+) (P = 0.008), ER+ PR+ (P = 0.007), and proliferation index (Ki-67) LT = 20 % (P = 0.036) tumors. Our findings suggest that miR-21 could be independent molecular marker of breast cancer invasiveness and potential target for future anti-miR therapies for the prevention of invasion and metastasis.
T2  - Medical Oncology
T1  - The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion
VL  - 31
IS  - 3
DO  - 10.1007/s12032-014-0867-x
ER  - 
@article{
author = "Petrović, Nina and Mandušić, Vesna and Stanojević, Boban and Lukić, Silvana and Todorović, Lidija and Roganović, Jelena and Dimitrijević, Bogomir B.",
year = "2014",
abstract = "MicroRNA-21 (miR-21) overexpression is characteristic for various types of tumors, but it is still unknown whether its expression levels differ between invasive and non-invasive breast carcinomas. The main goal of the study was to determine the difference in miR-21 expression among normal tissue, non-invasive, invasive with non-invasive component, and pure invasive breast cancer samples, to explain its potential role and significance in breast cancer invasiveness. The second goal was to propose miR-21 as molecular marker of breast cancer invasiveness and potential target for future anti-miR therapies for the prevention of invasion and metastasis. In order to reveal the role of miR-21 in breast cancer invasiveness, we measured miR-21 expression levels in 44 breast cancer and four normal samples by stem-loop real-time RT-PCR using TaqMan technology. Relative expression levels of miR-21 were significantly higher in invasive than in other groups (P = 0.002) and significantly higher in invasive compared with invasive with non-invasive component group in histological (P = 0.043) and nuclear grade 2 (P = 0.036), estrogen-receptor-positive (ER+) (P = 0.006), progesterone-receptor-positive (PR+) (P = 0.008), ER+ PR+ (P = 0.007), and proliferation index (Ki-67) LT = 20 % (P = 0.036) tumors. Our findings suggest that miR-21 could be independent molecular marker of breast cancer invasiveness and potential target for future anti-miR therapies for the prevention of invasion and metastasis.",
journal = "Medical Oncology",
title = "The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion",
volume = "31",
number = "3",
doi = "10.1007/s12032-014-0867-x"
}
Petrović, N., Mandušić, V., Stanojević, B., Lukić, S., Todorović, L., Roganović, J.,& Dimitrijević, B. B.. (2014). The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion. in Medical Oncology, 31(3).
https://doi.org/10.1007/s12032-014-0867-x
Petrović N, Mandušić V, Stanojević B, Lukić S, Todorović L, Roganović J, Dimitrijević BB. The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion. in Medical Oncology. 2014;31(3).
doi:10.1007/s12032-014-0867-x .
Petrović, Nina, Mandušić, Vesna, Stanojević, Boban, Lukić, Silvana, Todorović, Lidija, Roganović, Jelena, Dimitrijević, Bogomir B., "The difference in miR-21 expression levels between invasive and non-invasive breast cancers emphasizes its role in breast cancer invasion" in Medical Oncology, 31, no. 3 (2014),
https://doi.org/10.1007/s12032-014-0867-x . .
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