TY  - JOUR
AU  - Filipović Tričković, Jelena G.
AU  - Momčilović, Miloš
AU  - Joksić, Gordana
AU  - Živković, Sanja
AU  - Ilić, Bojana
AU  - Ognjanović, Miloš
AU  - Novaković, Mirjana
AU  - Valenta-Šobot, Ana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11376
AB  - Silver nanoparticles (AgNPs) have been recognized for widespread biological applications due to their antimicrobial and anti-inflammatory effect, especially in dentistry and for wound healing. Many features determine their beneficial or toxic potential, such as their synthesis type, size, morphology, coating, and concentration. Most synthesis types rely on the use of synthetic chemicals, which contributes to their toxicity. We present an environmentally friendly method for “green” synthesis of AgNPs from the silver target by pulsed laser ablation in liquid (PLAL) using citrate as the stabilizing agent. Since AgNPs already have many dental applications, we examined their antibacterial effect against supragingival biofilm-forming bacteria and bacterial strains known to cause resistant dental infections. Their impact on human fibroblast cells’ cytotoxicity, proliferation (measured by XTT and Ki-67 immunofluorescence), pro/antioxidant balance, and lipid peroxidation (measured by PAB and LPP) was evaluated. AgNPs1 (21 nm) and AgNPs2 (15 nm) spherical nanoparticles with good overall stability were obtained. The highest tested dose of smaller nanoparticles (AgNPs2) displays not only an effective antibacterial effect against the tested oral bacteria strains but also a pro-oxidant and cytotoxic effect on fibroblast cells. Lower doses do not affect bacterial survival but increase the cell proliferation and metabolic activity and show an antioxidative effect, suggesting that different concentrations display a substantially opposite effect. Compared to larger AgNPs1, smaller AgNPs2 possess more potent biological effects, indicating that size plays a pivotal role in their activity. Such opposite outcomes could be important for their medical application, and high concentrations could be used for the inhibition of dental biofilm formation and resistant dental infections as well as proliferative conditions, while low doses could be beneficial in the treatment of atrophic and inflammatory disorders. Finally, we showed that silver-targeted PLAL, using citrate as a stabilizing agent, produces biologically potent nanoparticles that could have many applications depending on their size and concentration.
T2  - Nanomaterials and Nanotechnology
T1  - Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects
VL  - 2023
SP  - e9854356
DO  - 10.1155/2023/9854356
ER  - 

@article{
author = "Filipović Tričković, Jelena G. and Momčilović, Miloš and Joksić, Gordana and Živković, Sanja and Ilić, Bojana and Ognjanović, Miloš and Novaković, Mirjana and Valenta-Šobot, Ana",
year = "2023",
abstract = "Silver nanoparticles (AgNPs) have been recognized for widespread biological applications due to their antimicrobial and anti-inflammatory effect, especially in dentistry and for wound healing. Many features determine their beneficial or toxic potential, such as their synthesis type, size, morphology, coating, and concentration. Most synthesis types rely on the use of synthetic chemicals, which contributes to their toxicity. We present an environmentally friendly method for “green” synthesis of AgNPs from the silver target by pulsed laser ablation in liquid (PLAL) using citrate as the stabilizing agent. Since AgNPs already have many dental applications, we examined their antibacterial effect against supragingival biofilm-forming bacteria and bacterial strains known to cause resistant dental infections. Their impact on human fibroblast cells’ cytotoxicity, proliferation (measured by XTT and Ki-67 immunofluorescence), pro/antioxidant balance, and lipid peroxidation (measured by PAB and LPP) was evaluated. AgNPs1 (21 nm) and AgNPs2 (15 nm) spherical nanoparticles with good overall stability were obtained. The highest tested dose of smaller nanoparticles (AgNPs2) displays not only an effective antibacterial effect against the tested oral bacteria strains but also a pro-oxidant and cytotoxic effect on fibroblast cells. Lower doses do not affect bacterial survival but increase the cell proliferation and metabolic activity and show an antioxidative effect, suggesting that different concentrations display a substantially opposite effect. Compared to larger AgNPs1, smaller AgNPs2 possess more potent biological effects, indicating that size plays a pivotal role in their activity. Such opposite outcomes could be important for their medical application, and high concentrations could be used for the inhibition of dental biofilm formation and resistant dental infections as well as proliferative conditions, while low doses could be beneficial in the treatment of atrophic and inflammatory disorders. Finally, we showed that silver-targeted PLAL, using citrate as a stabilizing agent, produces biologically potent nanoparticles that could have many applications depending on their size and concentration.",
journal = "Nanomaterials and Nanotechnology",
title = "Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects",
volume = "2023",
pages = "e9854356",
doi = "10.1155/2023/9854356"
}

Filipović Tričković, J. G., Momčilović, M., Joksić, G., Živković, S., Ilić, B., Ognjanović, M., Novaković, M.,& Valenta-Šobot, A.. (2023). Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects. in Nanomaterials and Nanotechnology, 2023, e9854356.
https://doi.org/10.1155/2023/9854356

Filipović Tričković JG, Momčilović M, Joksić G, Živković S, Ilić B, Ognjanović M, Novaković M, Valenta-Šobot A. Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects. in Nanomaterials and Nanotechnology. 2023;2023:e9854356.
doi:10.1155/2023/9854356 .

Filipović Tričković, Jelena G., Momčilović, Miloš, Joksić, Gordana, Živković, Sanja, Ilić, Bojana, Ognjanović, Miloš, Novaković, Mirjana, Valenta-Šobot, Ana, "Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects" in Nanomaterials and Nanotechnology, 2023 (2023):e9854356,
https://doi.org/10.1155/2023/9854356 . .

TY  - CONF
AU  - Ilić, Jana
AU  - Trišić, Dijana
AU  - Čolović, Božana
AU  - Ilić, Bojana
AU  - Filipović Tričković, Jelena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12812
AB  - Aim: The use of nitinol archwires (NiTi) in orthodontic treatment has expanded significantly due to unique
mechanical properties such as shape memory effect and superelasticity. The greatest barrier to safe
application of NiTi archwires is microbiologically or electrochemically induced corrosion. Corrosion of NiTi
archwires result in Ni2+ release, leading to different health implications. The purpose of this investigation is
the improvement of NiTi archwires properties by synthesis of multilayerd, firmly bonded nanocoatings with
antibacterial properties using the magnetron sputtering (MS). These nanocoatings will enhance corrosion
resistance and decrease cytotoxic properties by preventing Ni2+ release, without compromising mechanical
characteristics of the conventional NiTi archwires. Materials and Method: The nanocoatings of TiO2, TiN,
TiN+Cu were acquired by pulsed magnetron sputtering using the commercially available nickel-titanium
archwires. The samples were assessed using atomic force microscopy and X-ray diffraction. Inductively
coupled plasma - optical emission spectrometry was used to estimate the release of Ni, Ti, Cu, Fe, Cr, Zn
ions into DMEM and acidic solution. Biocompatibility of samples' eluates was investigated using Neutral red
staining. Disk Diffusion Method was used for assessing the antibacterial properties against bacterial
suspensions of Streptococcus mutans. Results: Compared to NiTi and stainless steel, the nonocoated
archwires showed statistically lower release of nickel both in DMEM and acidic environment (p<0.05).
Regarding the relative cell viability, the 7-day eluates of nanocoated archwires was the highest (p<0.05).
Antibacterial activity of nanocoated archwires was statistically significant in comparison to the conventional
NiTi and stainless steel archwires (p<0.05). Conclusions: As nanocoated archwires presented desired
antibacterial properties with better stability in both acidic and non-acidic conditions, as well as satisfactory biocompatibility, they may be a good candidate for further clinical investigations.
PB  - European Orthodontic Society
C3  - European Journal of Orthodontics
T1  - Novel Nanocoated Nitinol Archwires With Antibacterial Properties
VL  - 45
IS  - 6 - EOS2023 Abstracts
SP  - e162
EP  - e162
DO  - 10.1093/ejo/cjad072
ER  - 

@conference{
author = "Ilić, Jana and Trišić, Dijana and Čolović, Božana and Ilić, Bojana and Filipović Tričković, Jelena",
year = "2023",
abstract = "Aim: The use of nitinol archwires (NiTi) in orthodontic treatment has expanded significantly due to unique
mechanical properties such as shape memory effect and superelasticity. The greatest barrier to safe
application of NiTi archwires is microbiologically or electrochemically induced corrosion. Corrosion of NiTi
archwires result in Ni2+ release, leading to different health implications. The purpose of this investigation is
the improvement of NiTi archwires properties by synthesis of multilayerd, firmly bonded nanocoatings with
antibacterial properties using the magnetron sputtering (MS). These nanocoatings will enhance corrosion
resistance and decrease cytotoxic properties by preventing Ni2+ release, without compromising mechanical
characteristics of the conventional NiTi archwires. Materials and Method: The nanocoatings of TiO2, TiN,
TiN+Cu were acquired by pulsed magnetron sputtering using the commercially available nickel-titanium
archwires. The samples were assessed using atomic force microscopy and X-ray diffraction. Inductively
coupled plasma - optical emission spectrometry was used to estimate the release of Ni, Ti, Cu, Fe, Cr, Zn
ions into DMEM and acidic solution. Biocompatibility of samples' eluates was investigated using Neutral red
staining. Disk Diffusion Method was used for assessing the antibacterial properties against bacterial
suspensions of Streptococcus mutans. Results: Compared to NiTi and stainless steel, the nonocoated
archwires showed statistically lower release of nickel both in DMEM and acidic environment (p<0.05).
Regarding the relative cell viability, the 7-day eluates of nanocoated archwires was the highest (p<0.05).
Antibacterial activity of nanocoated archwires was statistically significant in comparison to the conventional
NiTi and stainless steel archwires (p<0.05). Conclusions: As nanocoated archwires presented desired
antibacterial properties with better stability in both acidic and non-acidic conditions, as well as satisfactory biocompatibility, they may be a good candidate for further clinical investigations.",
publisher = "European Orthodontic Society",
journal = "European Journal of Orthodontics",
title = "Novel Nanocoated Nitinol Archwires With Antibacterial Properties",
volume = "45",
number = "6 - EOS2023 Abstracts",
pages = "e162-e162",
doi = "10.1093/ejo/cjad072"
}

Ilić, J., Trišić, D., Čolović, B., Ilić, B.,& Filipović Tričković, J.. (2023). Novel Nanocoated Nitinol Archwires With Antibacterial Properties. in European Journal of Orthodontics
European Orthodontic Society., 45(6 - EOS2023 Abstracts), e162-e162.
https://doi.org/10.1093/ejo/cjad072

Ilić J, Trišić D, Čolović B, Ilić B, Filipović Tričković J. Novel Nanocoated Nitinol Archwires With Antibacterial Properties. in European Journal of Orthodontics. 2023;45(6 - EOS2023 Abstracts):e162-e162.
doi:10.1093/ejo/cjad072 .

Ilić, Jana, Trišić, Dijana, Čolović, Božana, Ilić, Bojana, Filipović Tričković, Jelena, "Novel Nanocoated Nitinol Archwires With Antibacterial Properties" in European Journal of Orthodontics, 45, no. 6 - EOS2023 Abstracts (2023):e162-e162,
https://doi.org/10.1093/ejo/cjad072 . .

TY  - JOUR
AU  - Ilić, Bojana
AU  - Petrović, Božana
AU  - Marinković, Jelena
AU  - Miletić Vukajlović, Jadranka
AU  - Stevanović, Momir
AU  - Potočnik, Jelena
AU  - Jokanović, Vukoman
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11624
AB  - Background: The use of nitinol (NiTi) archwires in orthodontic treatment has increased significantly due to unique mechanical properties. The greatest obstacle for safe orthodontic treatment is chemically or microbiologically induced corrosion, resulting in nickel (Ni) release. The aim of this investigation was to enhance corrosion resistance and introduce antibacterial properties to NiTi archwires by coating them with copper (Cu) doper titanium nitride (TiN-Cu). Methods: NiTi archwires were coated with TiN-Cu using cathodic arc evaporation (CAE) and direct current magnetron sputtering (DC-MS). The morphology of the sample was analyzed via field emission scanning electron microscopy (FESEM) and chemical composition was assessed using energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and Fourier transformed infrared spectroscopy (FTIR). Inductively coupled plasma optical emission spectrometry (ICP-OES) was used to estimate the ion release. The biocompatibility of samples was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) assay. Antibacterial activity was tested against Streptococcus mutans and Streptococcus mitis. Results: Physicochemical characterization revealed well-designed coatings with the presence of TiN phase with incorporated Cu. TiN-Cu-nanocoated archwires showed a statistically lower Ni release (p < 0.05). Relative cell viability was the highest in 28-day eluates of TiN-Cu-nanocoated archwires (p < 0.05). The most remarkable decrease in Streptococcus mitis concentrations was observed in the case of TiN-Cu-coated archwires (p < 0.05). Conclusion: Taking into account biocompatibility and antibacterial tests, TiN-Cu-nanocoated archwires may be considered as a good candidate for further clinical investigations
T2  - Coatings
T1  - Investigation of Ion Release and Antibacterial Properties of TiN-Cu-Nanocoated Nitinol Archwires
VL  - 13
IS  - 9
SP  - 1587
DO  - 10.3390/coatings13091587
ER  - 

@article{
author = "Ilić, Bojana and Petrović, Božana and Marinković, Jelena and Miletić Vukajlović, Jadranka and Stevanović, Momir and Potočnik, Jelena and Jokanović, Vukoman",
year = "2023",
abstract = "Background: The use of nitinol (NiTi) archwires in orthodontic treatment has increased significantly due to unique mechanical properties. The greatest obstacle for safe orthodontic treatment is chemically or microbiologically induced corrosion, resulting in nickel (Ni) release. The aim of this investigation was to enhance corrosion resistance and introduce antibacterial properties to NiTi archwires by coating them with copper (Cu) doper titanium nitride (TiN-Cu). Methods: NiTi archwires were coated with TiN-Cu using cathodic arc evaporation (CAE) and direct current magnetron sputtering (DC-MS). The morphology of the sample was analyzed via field emission scanning electron microscopy (FESEM) and chemical composition was assessed using energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and Fourier transformed infrared spectroscopy (FTIR). Inductively coupled plasma optical emission spectrometry (ICP-OES) was used to estimate the ion release. The biocompatibility of samples was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) assay. Antibacterial activity was tested against Streptococcus mutans and Streptococcus mitis. Results: Physicochemical characterization revealed well-designed coatings with the presence of TiN phase with incorporated Cu. TiN-Cu-nanocoated archwires showed a statistically lower Ni release (p < 0.05). Relative cell viability was the highest in 28-day eluates of TiN-Cu-nanocoated archwires (p < 0.05). The most remarkable decrease in Streptococcus mitis concentrations was observed in the case of TiN-Cu-coated archwires (p < 0.05). Conclusion: Taking into account biocompatibility and antibacterial tests, TiN-Cu-nanocoated archwires may be considered as a good candidate for further clinical investigations",
journal = "Coatings",
title = "Investigation of Ion Release and Antibacterial Properties of TiN-Cu-Nanocoated Nitinol Archwires",
volume = "13",
number = "9",
pages = "1587",
doi = "10.3390/coatings13091587"
}

Ilić, B., Petrović, B., Marinković, J., Miletić Vukajlović, J., Stevanović, M., Potočnik, J.,& Jokanović, V.. (2023). Investigation of Ion Release and Antibacterial Properties of TiN-Cu-Nanocoated Nitinol Archwires. in Coatings, 13(9), 1587.
https://doi.org/10.3390/coatings13091587

Ilić B, Petrović B, Marinković J, Miletić Vukajlović J, Stevanović M, Potočnik J, Jokanović V. Investigation of Ion Release and Antibacterial Properties of TiN-Cu-Nanocoated Nitinol Archwires. in Coatings. 2023;13(9):1587.
doi:10.3390/coatings13091587 .

Ilić, Bojana, Petrović, Božana, Marinković, Jelena, Miletić Vukajlović, Jadranka, Stevanović, Momir, Potočnik, Jelena, Jokanović, Vukoman, "Investigation of Ion Release and Antibacterial Properties of TiN-Cu-Nanocoated Nitinol Archwires" in Coatings, 13, no. 9 (2023):1587,
https://doi.org/10.3390/coatings13091587 . .

TY  - JOUR
AU  - Trišić, Dijana
AU  - Ćetenović, Bojana
AU  - Zdravković, Nemanja M.
AU  - Marković, Tatjana
AU  - Dojčinović, Biljana P.
AU  - Jokanović, Vukoman R.
AU  - Marković, Dejan
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8426
AB  - Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA+) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the antibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA+ had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA+ in 3h and 24h of the incubation period (p < 0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA+. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution. © 2019, Inst. Sci. inf., Univ. Defence in Belgrade. All rights reserved.
T2  - Vojnosanitetski pregled
T1  - Antibacterial effects of new endodontic materials based on calcium silicates
VL  - 76
IS  - 4
SP  - 365
EP  - 372
DO  - 10.2298/VSP161231130T
ER  - 

@article{
author = "Trišić, Dijana and Ćetenović, Bojana and Zdravković, Nemanja M. and Marković, Tatjana and Dojčinović, Biljana P. and Jokanović, Vukoman R. and Marković, Dejan",
year = "2019",
abstract = "Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA+) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the antibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA+ had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA+ in 3h and 24h of the incubation period (p < 0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA+. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution. © 2019, Inst. Sci. inf., Univ. Defence in Belgrade. All rights reserved.",
journal = "Vojnosanitetski pregled",
title = "Antibacterial effects of new endodontic materials based on calcium silicates",
volume = "76",
number = "4",
pages = "365-372",
doi = "10.2298/VSP161231130T"
}

Trišić, D., Ćetenović, B., Zdravković, N. M., Marković, T., Dojčinović, B. P., Jokanović, V. R.,& Marković, D.. (2019). Antibacterial effects of new endodontic materials based on calcium silicates. in Vojnosanitetski pregled, 76(4), 365-372.
https://doi.org/10.2298/VSP161231130T

Trišić D, Ćetenović B, Zdravković NM, Marković T, Dojčinović BP, Jokanović VR, Marković D. Antibacterial effects of new endodontic materials based on calcium silicates. in Vojnosanitetski pregled. 2019;76(4):365-372.
doi:10.2298/VSP161231130T .

Trišić, Dijana, Ćetenović, Bojana, Zdravković, Nemanja M., Marković, Tatjana, Dojčinović, Biljana P., Jokanović, Vukoman R., Marković, Dejan, "Antibacterial effects of new endodontic materials based on calcium silicates" in Vojnosanitetski pregled, 76, no. 4 (2019):365-372,
https://doi.org/10.2298/VSP161231130T . .

TY  - JOUR
AU  - Ćetenović, Bojana
AU  - Marković, Dejan
AU  - Gutmann, James
AU  - Perić, Tamara
AU  - Jokanović, Vukoman R.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8455
AB  - Introduction/Objective The purpose of this study was to assess effectiveness of endodontic root canal procedures in traumatized permanent teeth with necrotic pulps and chronic periapical lesions after definitive obturation with mineral trioxide aggregate (MTA) products. Adobe Photoshop CS (San Jose, CA, USA) image-analysis software was used for healing assessment. Methods Twenty-seven traumatized single-rooted permanent teeth diagnosed with necrotic pulps and chronic periapical lesions were treated with non-surgical procedure using a tri-antibiotic paste and calcium hydroxide as intra-canal medication. Definitive obturation was performed with ProRoot MTA (Dentsply Tulsa Dental Specialties, Tulsa, OK, USA) or MTA+ Cerkamed (Cerkamed, Stalowa Wola, Poland). Control follow-ups were done three, six, 12, and 24 months following the completion of treatment. Results The positive clinical outcome was recorded in 24 (88.9%) cases, while radiographic success was present in 26 (96.3%) cases. A statistically significant decrease in the sizes of periapical lesions was consistently observed at follow-up periods (p < 0.001). There was no statistically significant difference between the two tested MTA materials (p > 0.05). Conclusion The MTA products were effective for the root canal obturation and appeared to contribute to the significant reduction or complete regression of periapical lesions in teeth treated. The presented procedure may be proposed for everyday clinical practice. © 2019, Serbia Medical Society. All rights reserved.
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation - a preliminary study
VL  - 147
IS  - 5-6
SP  - 270
EP  - 275
DO  - 10.2298/SARH180301018C
ER  - 

@article{
author = "Ćetenović, Bojana and Marković, Dejan and Gutmann, James and Perić, Tamara and Jokanović, Vukoman R.",
year = "2019",
abstract = "Introduction/Objective The purpose of this study was to assess effectiveness of endodontic root canal procedures in traumatized permanent teeth with necrotic pulps and chronic periapical lesions after definitive obturation with mineral trioxide aggregate (MTA) products. Adobe Photoshop CS (San Jose, CA, USA) image-analysis software was used for healing assessment. Methods Twenty-seven traumatized single-rooted permanent teeth diagnosed with necrotic pulps and chronic periapical lesions were treated with non-surgical procedure using a tri-antibiotic paste and calcium hydroxide as intra-canal medication. Definitive obturation was performed with ProRoot MTA (Dentsply Tulsa Dental Specialties, Tulsa, OK, USA) or MTA+ Cerkamed (Cerkamed, Stalowa Wola, Poland). Control follow-ups were done three, six, 12, and 24 months following the completion of treatment. Results The positive clinical outcome was recorded in 24 (88.9%) cases, while radiographic success was present in 26 (96.3%) cases. A statistically significant decrease in the sizes of periapical lesions was consistently observed at follow-up periods (p < 0.001). There was no statistically significant difference between the two tested MTA materials (p > 0.05). Conclusion The MTA products were effective for the root canal obturation and appeared to contribute to the significant reduction or complete regression of periapical lesions in teeth treated. The presented procedure may be proposed for everyday clinical practice. © 2019, Serbia Medical Society. All rights reserved.",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation - a preliminary study",
volume = "147",
number = "5-6",
pages = "270-275",
doi = "10.2298/SARH180301018C"
}

Ćetenović, B., Marković, D., Gutmann, J., Perić, T.,& Jokanović, V. R.. (2019). Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation - a preliminary study. in Srpski arhiv za celokupno lekarstvo, 147(5-6), 270-275.
https://doi.org/10.2298/SARH180301018C

Ćetenović B, Marković D, Gutmann J, Perić T, Jokanović VR. Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation - a preliminary study. in Srpski arhiv za celokupno lekarstvo. 2019;147(5-6):270-275.
doi:10.2298/SARH180301018C .

Ćetenović, Bojana, Marković, Dejan, Gutmann, James, Perić, Tamara, Jokanović, Vukoman R., "Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation - a preliminary study" in Srpski arhiv za celokupno lekarstvo, 147, no. 5-6 (2019):270-275,
https://doi.org/10.2298/SARH180301018C . .

TY  - JOUR
AU  - Ćetenović, Bojana
AU  - Čolović, Božana M.
AU  - Vasilijić, Saša
AU  - Prokić, Bogomir
AU  - Pašalić, Snežana
AU  - Jokanović, Vukoman R.
AU  - Tepavčević, Zvezdana
AU  - Marković, Dejan
PY  - 2018
UR  - http://link.springer.com/10.1007/s10856-018-6200-z
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7990
AB  - The aim of this study was to investigate the biocompatibility of nanostructured materials based on highly active calcium silicates mixed with different radiocontrast agents in comparison to MTA+ using in vitro and in vivo model. Morphology of materials’ samples was analyzed using SEM while the phase compositions were identified by XRD. pH values of materials’ suspensions were conducted by pH-meter. The cytotoxicity of materials’ solutions was tested by MTT test (100, 50, 25 and 12.5 mg/ml). LDH and 3H-thymidine assay were utilized for biocompatibility investigations of materials’ eluates (24 h, 7 day and 21 day). Eighteen Guinea pigs were used for intramuscular implantation, as teflon tubes with freshly prepared materials were placed into intramuscular pockets. All samples were composed of round and needle-like particles equally distributed with Ca/Si ratio ~2.7 at%, with the presence of hydrated calcium silicate phases. The pH values of ALBO-MPCA1 and ALBO-MPCA2 were high alkaline, while in case of MTA+ they were lower and continuously declined (p < 0.05). Investigated materials didn’t exhibit dose-dependent effect on metabolic activity of L929 cells (p > 0.05). Significant differences in the percentage of cytotoxicity between diluted and undiluted extracts between all tested materials after 24 h and 7 day were noticed (p < 0.05). Increase in L929 cells proliferation was noticed in case of undiluted eluates of ALBO-MPCA1 and ALBO-MPCA2 after 7 day (p < 0.05). There were no statistically significant differences in the intensity of inflammatory response between investigated materials and control group after 60 day (p > 0.05). Evaluation of biocompatibility of both ALBO-MPCA1 and ALBO-MPCA2 indicate their potential clinical use. [Figure not available: see fulltext.]. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Journal of Materials Science: Materials in Medicine
T1  - Nanostructured endodontic materials mixed with different radiocontrast agents — biocompatibility study
VL  - 29
IS  - 12
SP  - 190
DO  - 10.1007/s10856-018-6200-z
ER  - 

@article{
author = "Ćetenović, Bojana and Čolović, Božana M. and Vasilijić, Saša and Prokić, Bogomir and Pašalić, Snežana and Jokanović, Vukoman R. and Tepavčević, Zvezdana and Marković, Dejan",
year = "2018",
abstract = "The aim of this study was to investigate the biocompatibility of nanostructured materials based on highly active calcium silicates mixed with different radiocontrast agents in comparison to MTA+ using in vitro and in vivo model. Morphology of materials’ samples was analyzed using SEM while the phase compositions were identified by XRD. pH values of materials’ suspensions were conducted by pH-meter. The cytotoxicity of materials’ solutions was tested by MTT test (100, 50, 25 and 12.5 mg/ml). LDH and 3H-thymidine assay were utilized for biocompatibility investigations of materials’ eluates (24 h, 7 day and 21 day). Eighteen Guinea pigs were used for intramuscular implantation, as teflon tubes with freshly prepared materials were placed into intramuscular pockets. All samples were composed of round and needle-like particles equally distributed with Ca/Si ratio ~2.7 at%, with the presence of hydrated calcium silicate phases. The pH values of ALBO-MPCA1 and ALBO-MPCA2 were high alkaline, while in case of MTA+ they were lower and continuously declined (p < 0.05). Investigated materials didn’t exhibit dose-dependent effect on metabolic activity of L929 cells (p > 0.05). Significant differences in the percentage of cytotoxicity between diluted and undiluted extracts between all tested materials after 24 h and 7 day were noticed (p < 0.05). Increase in L929 cells proliferation was noticed in case of undiluted eluates of ALBO-MPCA1 and ALBO-MPCA2 after 7 day (p < 0.05). There were no statistically significant differences in the intensity of inflammatory response between investigated materials and control group after 60 day (p > 0.05). Evaluation of biocompatibility of both ALBO-MPCA1 and ALBO-MPCA2 indicate their potential clinical use. [Figure not available: see fulltext.]. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Journal of Materials Science: Materials in Medicine",
title = "Nanostructured endodontic materials mixed with different radiocontrast agents — biocompatibility study",
volume = "29",
number = "12",
pages = "190",
doi = "10.1007/s10856-018-6200-z"
}

Ćetenović, B., Čolović, B. M., Vasilijić, S., Prokić, B., Pašalić, S., Jokanović, V. R., Tepavčević, Z.,& Marković, D.. (2018). Nanostructured endodontic materials mixed with different radiocontrast agents — biocompatibility study. in Journal of Materials Science: Materials in Medicine, 29(12), 190.
https://doi.org/10.1007/s10856-018-6200-z

Ćetenović B, Čolović BM, Vasilijić S, Prokić B, Pašalić S, Jokanović VR, Tepavčević Z, Marković D. Nanostructured endodontic materials mixed with different radiocontrast agents — biocompatibility study. in Journal of Materials Science: Materials in Medicine. 2018;29(12):190.
doi:10.1007/s10856-018-6200-z .

Ćetenović, Bojana, Čolović, Božana M., Vasilijić, Saša, Prokić, Bogomir, Pašalić, Snežana, Jokanović, Vukoman R., Tepavčević, Zvezdana, Marković, Dejan, "Nanostructured endodontic materials mixed with different radiocontrast agents — biocompatibility study" in Journal of Materials Science: Materials in Medicine, 29, no. 12 (2018):190,
https://doi.org/10.1007/s10856-018-6200-z . .

TY  - JOUR
AU  - Ćetenović, Bojana
AU  - Prokić, Bogomir
AU  - Vasilijić, Saša
AU  - Dojčinović, Biljana P.
AU  - Magic, Marko
AU  - Jokanović, Vukoman R.
AU  - Marković, Dejan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1451
AB  - Introduction: The aim of this article was to analyze biocompatibility and bioactivity of new endodontic materials on the basis of nanosynthesized calcium silicates (ALBO-MPCA(1) and ALBO-MPCA(2)) combined with different radiopacifiers in comparison with MTA(+). Methods: Morphology of the samples was studied by scanning electron microscopy, and the pH and ion release analysis were also assessed. Biocompatibility of materials eluates (24-hour, 7-day, and 21-day) was conducted by using MIT test. Twelve New Zealand white rabbits were used for intraosseous implantation. Four calvarial defects per animal were created and filled with freshly prepared investigated materials. Results: Samples mostly consisted of agglomerates built up from nanoparticles, preferably spherical and rod-like. There was no significant difference among pH values of materials eluates after 24 hours (P GT .05). The amount of calcium and aluminum ion release decreased, whereas the amount of magnesium and bismuth (ALBO-MPCAl, MTA(+)) and barium (ALBO-MPCA(2)) increased during 21-day period. The metabolic activity of cells increased after the extraction time, except in case of undiluted elutes of ALBO-MPCA(2) and ALBO-MPCAI (21-day). Histologic analysis of the samples revealed newly formed bone tissue with moderate inflammation for all investigated materials, which subsided during 90-day period to mild. Both MIA(+) and ALBO-MPCAI were in direct contact with the newly formed bone tissue. After 90 days, statistically significant difference in hard tissue formation was observed in comparison of MIA(+) and ALBO-MPCki with control group (P LT .05). Conclusions: Experimental materials ALBOMPCA, and ALBO-MPCA(2) possess both biocompatibility and bioactivity. Because ALBO-MPCAA provokes favorable biological response, it is especially good candidate for further clinical investigations.
T2  - Journal of Endodontics
T1  - Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers
VL  - 43
IS  - 3
SP  - 425
EP  - 432
DO  - 10.1016/j.joen.2016.10.041
ER  - 

@article{
author = "Ćetenović, Bojana and Prokić, Bogomir and Vasilijić, Saša and Dojčinović, Biljana P. and Magic, Marko and Jokanović, Vukoman R. and Marković, Dejan",
year = "2017",
abstract = "Introduction: The aim of this article was to analyze biocompatibility and bioactivity of new endodontic materials on the basis of nanosynthesized calcium silicates (ALBO-MPCA(1) and ALBO-MPCA(2)) combined with different radiopacifiers in comparison with MTA(+). Methods: Morphology of the samples was studied by scanning electron microscopy, and the pH and ion release analysis were also assessed. Biocompatibility of materials eluates (24-hour, 7-day, and 21-day) was conducted by using MIT test. Twelve New Zealand white rabbits were used for intraosseous implantation. Four calvarial defects per animal were created and filled with freshly prepared investigated materials. Results: Samples mostly consisted of agglomerates built up from nanoparticles, preferably spherical and rod-like. There was no significant difference among pH values of materials eluates after 24 hours (P GT .05). The amount of calcium and aluminum ion release decreased, whereas the amount of magnesium and bismuth (ALBO-MPCAl, MTA(+)) and barium (ALBO-MPCA(2)) increased during 21-day period. The metabolic activity of cells increased after the extraction time, except in case of undiluted elutes of ALBO-MPCA(2) and ALBO-MPCAI (21-day). Histologic analysis of the samples revealed newly formed bone tissue with moderate inflammation for all investigated materials, which subsided during 90-day period to mild. Both MIA(+) and ALBO-MPCAI were in direct contact with the newly formed bone tissue. After 90 days, statistically significant difference in hard tissue formation was observed in comparison of MIA(+) and ALBO-MPCki with control group (P LT .05). Conclusions: Experimental materials ALBOMPCA, and ALBO-MPCA(2) possess both biocompatibility and bioactivity. Because ALBO-MPCAA provokes favorable biological response, it is especially good candidate for further clinical investigations.",
journal = "Journal of Endodontics",
title = "Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers",
volume = "43",
number = "3",
pages = "425-432",
doi = "10.1016/j.joen.2016.10.041"
}

Ćetenović, B., Prokić, B., Vasilijić, S., Dojčinović, B. P., Magic, M., Jokanović, V. R.,& Marković, D.. (2017). Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers. in Journal of Endodontics, 43(3), 425-432.
https://doi.org/10.1016/j.joen.2016.10.041

Ćetenović B, Prokić B, Vasilijić S, Dojčinović BP, Magic M, Jokanović VR, Marković D. Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers. in Journal of Endodontics. 2017;43(3):425-432.
doi:10.1016/j.joen.2016.10.041 .

Ćetenović, Bojana, Prokić, Bogomir, Vasilijić, Saša, Dojčinović, Biljana P., Magic, Marko, Jokanović, Vukoman R., Marković, Dejan, "Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers" in Journal of Endodontics, 43, no. 3 (2017):425-432,
https://doi.org/10.1016/j.joen.2016.10.041 . .

TY  - JOUR
AU  - Jokanović, Vukoman R.
AU  - Čolović, Božana M.
AU  - Mitrić, Miodrag
AU  - Marković, Dejan
AU  - Ćetenović, Bojana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5833
AB  - A synthesis of a new dental material, based on nano-structured highly active calcium silicates and calcium carbonates, is described in this paper. Phase analysis of this material, before and after hydration, was performed by X-ray diffraction and showed that active silicate phases were transformed into tobermorite phase, while carbonates remained unchanged. The mechanism of hydration was fully described. The morphology of the sample was studied by SEM, and typical appearance of the present phases was particularly discussed. The mechanical properties and setting time of this material make it very promising for potential application in dental practice.
T2  - International Journal of Applied Ceramic Technology
T1  - Synthesis and Properties of a New Dental Material Based on Nano-Structured Highly Active Calcium Silicates and Calcium Carbonates
VL  - 11
IS  - 1
SP  - 57
EP  - 64
DO  - 10.1111/ijac.12070
ER  - 

@article{
author = "Jokanović, Vukoman R. and Čolović, Božana M. and Mitrić, Miodrag and Marković, Dejan and Ćetenović, Bojana",
year = "2014",
abstract = "A synthesis of a new dental material, based on nano-structured highly active calcium silicates and calcium carbonates, is described in this paper. Phase analysis of this material, before and after hydration, was performed by X-ray diffraction and showed that active silicate phases were transformed into tobermorite phase, while carbonates remained unchanged. The mechanism of hydration was fully described. The morphology of the sample was studied by SEM, and typical appearance of the present phases was particularly discussed. The mechanical properties and setting time of this material make it very promising for potential application in dental practice.",
journal = "International Journal of Applied Ceramic Technology",
title = "Synthesis and Properties of a New Dental Material Based on Nano-Structured Highly Active Calcium Silicates and Calcium Carbonates",
volume = "11",
number = "1",
pages = "57-64",
doi = "10.1111/ijac.12070"
}

Jokanović, V. R., Čolović, B. M., Mitrić, M., Marković, D.,& Ćetenović, B.. (2014). Synthesis and Properties of a New Dental Material Based on Nano-Structured Highly Active Calcium Silicates and Calcium Carbonates. in International Journal of Applied Ceramic Technology, 11(1), 57-64.
https://doi.org/10.1111/ijac.12070

Jokanović VR, Čolović BM, Mitrić M, Marković D, Ćetenović B. Synthesis and Properties of a New Dental Material Based on Nano-Structured Highly Active Calcium Silicates and Calcium Carbonates. in International Journal of Applied Ceramic Technology. 2014;11(1):57-64.
doi:10.1111/ijac.12070 .

Jokanović, Vukoman R., Čolović, Božana M., Mitrić, Miodrag, Marković, Dejan, Ćetenović, Bojana, "Synthesis and Properties of a New Dental Material Based on Nano-Structured Highly Active Calcium Silicates and Calcium Carbonates" in International Journal of Applied Ceramic Technology, 11, no. 1 (2014):57-64,
https://doi.org/10.1111/ijac.12070 . .

TY  - JOUR
AU  - Ćetenović, Bojana
AU  - Marković, Dejan
AU  - Petrovic, Bojan
AU  - Peric, Tamara
AU  - Jokanović, Vukoman R.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5854
AB  - Introduction. Dental injuries in immature permanent teeth often result in endodontic complications. Apexification technique using calcium hydroxide is associated with certain flaws, such as long treatment time, the possibility of tooth fracture and incomplete calcification. The use of an apical plug employing mineral trioxide aggregate (MTA) is an alternative treatment option. Case report. We reported the successful treatment of 4 maxillary incisors (in a 7-year-old boy and a 10-year-old girl) with open apices and periapical lesions. Apical portions of the canals were filled with MTA plugs in both cases. Coronal parts of the root canals were filled with gutta-percha and sealer. Clinical findings were clear 6 months after the definite obturation with no pathological changes on the radiographs in both cases. Conclusion. The use of MTA for apical plugging appears to be a valid treatment option in traumatized immature teeth with endodontic complications.
T2  - Vojnosanitetski pregled
T1  - Use of mineral trioxide aggregate in the treatement of traumatized teeth in children - Two case reports
VL  - 70
IS  - 8
SP  - 781
EP  - 784
DO  - 10.2298/VSP1308781C
ER  - 

@article{
author = "Ćetenović, Bojana and Marković, Dejan and Petrovic, Bojan and Peric, Tamara and Jokanović, Vukoman R.",
year = "2013",
abstract = "Introduction. Dental injuries in immature permanent teeth often result in endodontic complications. Apexification technique using calcium hydroxide is associated with certain flaws, such as long treatment time, the possibility of tooth fracture and incomplete calcification. The use of an apical plug employing mineral trioxide aggregate (MTA) is an alternative treatment option. Case report. We reported the successful treatment of 4 maxillary incisors (in a 7-year-old boy and a 10-year-old girl) with open apices and periapical lesions. Apical portions of the canals were filled with MTA plugs in both cases. Coronal parts of the root canals were filled with gutta-percha and sealer. Clinical findings were clear 6 months after the definite obturation with no pathological changes on the radiographs in both cases. Conclusion. The use of MTA for apical plugging appears to be a valid treatment option in traumatized immature teeth with endodontic complications.",
journal = "Vojnosanitetski pregled",
title = "Use of mineral trioxide aggregate in the treatement of traumatized teeth in children - Two case reports",
volume = "70",
number = "8",
pages = "781-784",
doi = "10.2298/VSP1308781C"
}

Ćetenović, B., Marković, D., Petrovic, B., Peric, T.,& Jokanović, V. R.. (2013). Use of mineral trioxide aggregate in the treatement of traumatized teeth in children - Two case reports. in Vojnosanitetski pregled, 70(8), 781-784.
https://doi.org/10.2298/VSP1308781C

Ćetenović B, Marković D, Petrovic B, Peric T, Jokanović VR. Use of mineral trioxide aggregate in the treatement of traumatized teeth in children - Two case reports. in Vojnosanitetski pregled. 2013;70(8):781-784.
doi:10.2298/VSP1308781C .

Ćetenović, Bojana, Marković, Dejan, Petrovic, Bojan, Peric, Tamara, Jokanović, Vukoman R., "Use of mineral trioxide aggregate in the treatement of traumatized teeth in children - Two case reports" in Vojnosanitetski pregled, 70, no. 8 (2013):781-784,
https://doi.org/10.2298/VSP1308781C . .