TY  - JOUR
AU  - Šaraba, Vladimir
AU  - Milovanović, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Budin, Clémence
AU  - de Boer, Tjalf
AU  - Ćirić, Milica
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12667
AB  - The selected brackish groundwater occurrences in the geotectonic regions of Inner Dinarides of western Serbia (Obrenovačka Banja) and Serbian crystalline core (Lomnički Kiseljak and Velika Vrbnica) were sampled for isolation and identification of plastic- and lignocellulose-degrading bacteria, as well as for the assessment of their enzymatic potential. The examined occurrences belong to the cold and warm (subthermal), weakly alkaline, neutral, and weakly acidic groundwater, and their genetic types are HCO3–Na + K and HCO3–Ca, Mg. The most abundant genera identified by next-generation 16S sequencing of cultivated groundwater samples belong to Aeromonas and Exiguobacterium. Of isolates screened on plastic and lignocellulosic substrates, 85.3% demonstrated growth and/or degrading activity on at least one tested substrate, with 27.8% isolates degrading plastic substrate Impranil® DLN-SD (SD), 1.9% plastic substrate bis(2-hydroxyethyl)terephthalate, and 5.6% carboxymethyl cellulose (CMC). Isolates degrading SD that were identified by 16S rDNA sequencing belonged to genera Stenotrophomonas, Flavobacterium, Pantoea, Enterobacter, Pseudomonas, Serratia, Acinetobacter, and Proteus, while isolates degrading CMC belonged to genera Rhizobium and Shewanella. All investigated brackish groundwaters harbor bacteria with potential in degradation of plastics or cellulose. Taking into account that microplastics contamination of groundwater resources is becoming a significant problem, the finding of plastic-degrading bacteria may have potential in bioremediation treatments of polluted groundwater. Subterranean ecosystems, which are largely untapped resources of biotechnologically relevant enzymes, are not traditionally considered the environment of choice for screening for plastic- and cellulose-degrading bacteria and therefore deserve a special attention from this aspect.
T2  - Microbial Ecology
T1  - Brackish Groundwaters Contain Plastic- and Cellulose-Degrading Bacteria
VL  - 86
IS  - 4
SP  - 2747
EP  - 2755
DO  - 10.1007/s00248-023-02278-8
ER  - 

@article{
author = "Šaraba, Vladimir and Milovanović, Jelena and Nikodinović-Runić, Jasmina and Budin, Clémence and de Boer, Tjalf and Ćirić, Milica",
year = "2023",
abstract = "The selected brackish groundwater occurrences in the geotectonic regions of Inner Dinarides of western Serbia (Obrenovačka Banja) and Serbian crystalline core (Lomnički Kiseljak and Velika Vrbnica) were sampled for isolation and identification of plastic- and lignocellulose-degrading bacteria, as well as for the assessment of their enzymatic potential. The examined occurrences belong to the cold and warm (subthermal), weakly alkaline, neutral, and weakly acidic groundwater, and their genetic types are HCO3–Na + K and HCO3–Ca, Mg. The most abundant genera identified by next-generation 16S sequencing of cultivated groundwater samples belong to Aeromonas and Exiguobacterium. Of isolates screened on plastic and lignocellulosic substrates, 85.3% demonstrated growth and/or degrading activity on at least one tested substrate, with 27.8% isolates degrading plastic substrate Impranil® DLN-SD (SD), 1.9% plastic substrate bis(2-hydroxyethyl)terephthalate, and 5.6% carboxymethyl cellulose (CMC). Isolates degrading SD that were identified by 16S rDNA sequencing belonged to genera Stenotrophomonas, Flavobacterium, Pantoea, Enterobacter, Pseudomonas, Serratia, Acinetobacter, and Proteus, while isolates degrading CMC belonged to genera Rhizobium and Shewanella. All investigated brackish groundwaters harbor bacteria with potential in degradation of plastics or cellulose. Taking into account that microplastics contamination of groundwater resources is becoming a significant problem, the finding of plastic-degrading bacteria may have potential in bioremediation treatments of polluted groundwater. Subterranean ecosystems, which are largely untapped resources of biotechnologically relevant enzymes, are not traditionally considered the environment of choice for screening for plastic- and cellulose-degrading bacteria and therefore deserve a special attention from this aspect.",
journal = "Microbial Ecology",
title = "Brackish Groundwaters Contain Plastic- and Cellulose-Degrading Bacteria",
volume = "86",
number = "4",
pages = "2747-2755",
doi = "10.1007/s00248-023-02278-8"
}

Šaraba, V., Milovanović, J., Nikodinović-Runić, J., Budin, C., de Boer, T.,& Ćirić, M.. (2023). Brackish Groundwaters Contain Plastic- and Cellulose-Degrading Bacteria. in Microbial Ecology, 86(4), 2747-2755.
https://doi.org/10.1007/s00248-023-02278-8

Šaraba V, Milovanović J, Nikodinović-Runić J, Budin C, de Boer T, Ćirić M. Brackish Groundwaters Contain Plastic- and Cellulose-Degrading Bacteria. in Microbial Ecology. 2023;86(4):2747-2755.
doi:10.1007/s00248-023-02278-8 .

Šaraba, Vladimir, Milovanović, Jelena, Nikodinović-Runić, Jasmina, Budin, Clémence, de Boer, Tjalf, Ćirić, Milica, "Brackish Groundwaters Contain Plastic- and Cellulose-Degrading Bacteria" in Microbial Ecology, 86, no. 4 (2023):2747-2755,
https://doi.org/10.1007/s00248-023-02278-8 . .

TY  - CONF
AU  - Todorović, Lidija
AU  - Milovanović, Jelena
AU  - Mandušić, Vesna
AU  - Živaljević, Vladan
AU  - Paunović, Ivan
AU  - Stanojević, Boban
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12634
AB  - Background: Thyroid neoplasms include benign tumors – thyroid adenoma (TA), and malignant tumors of various histological types: papillary thyroid carcinoma (PTC) – the most common and usually indolent, anaplasƟ c thyroid carcinoma (ATC) – the most aggressive, and several other types such as follicular, medullary and poorly diff erenƟ ated. Despite the progress in understanding the epidemiology and geneƟ c landscape of thyroid tumors, the diagnosis, prognosis and treatment approach require further improvement. Interleukin-6 (IL-6) is a pro-infl ammatory cytokine with a central role in the regulaƟ on of immune and infl ammatory responses including autoimmune thyroid diseases. Studies have revealed a potenƟ al impact of IL-6 in the development, progression and control of thyroid cancer. The aim of this study was to provide novel aspects for the preoperaƟ ve diff erenƟ al diagnosis and/or prognosis of thyroid cancer. To achieve this, we assessed the circulaƟ ng levels of IL-6 in paƟ ents with benign and malignant thyroid tumors of various histotypes, compared them with healthy volunteers, and correlated the results with clinicopathological parameters. PaƟ ents and Methods: The study included 43 paƟ ents with benign or malignant thyroid tumors, surgically treated at the Center for Endocrine Surgery, Clinical Center of Serbia. IL-6 protein levels were determined in plasma samples by quanƟ taƟ ve ELISA. Parametric and nonparametric staƟ sƟ cal tests were used for data analysis. Results: IL-6 concentraƟ ons in paƟ ents with either TA or carcinoma (PTC, ATC) were signifi cantly higher compared to the healthy volunteers (Mann Whitney test). The highest concentraƟ ons were detected in ATC paƟ ents (Median±SD 15.97±0.71 pg/mL), being signifi cantly higher compared to TA and PTC (2.14±1.34 pg/mL and 1.96±2.12 pg/mL, respecƟ vely). In PTC microcarcinoma, IL-6 was higher compared to controls, but there was no signifi cant diff erence compared to other PTC or TA (Mann Whitney test). The correlaƟ on analysis with clinicopathological parameters in PTC paƟ ents revealed a trend towards the associaƟ on of increased IL-6 plasma levels with the presence of nodal and distant metastases. No other signifi cant associaƟ ons were found. Conclusion: PaƟ ents with thyroid adenoma or carcinoma have increased plasma IL-6 levels that are in proporƟ on with the aggressiveness of the thyroid tumor, suggesƟ ng that IL-6 might be a candidate biomarker for diagnosis and prognosis of thyroid neoplasms.
PB  - Belgrade : Serbian Association for Cancer Research
C3  - Oncology Insights
T1  - Interleukin-6, a potential plasma biomarker for diagnosis and prognosis of thyroid neoplasms
IS  - 1
SP  - 83
EP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12634
ER  - 

@conference{
author = "Todorović, Lidija and Milovanović, Jelena and Mandušić, Vesna and Živaljević, Vladan and Paunović, Ivan and Stanojević, Boban",
year = "2023",
abstract = "Background: Thyroid neoplasms include benign tumors – thyroid adenoma (TA), and malignant tumors of various histological types: papillary thyroid carcinoma (PTC) – the most common and usually indolent, anaplasƟ c thyroid carcinoma (ATC) – the most aggressive, and several other types such as follicular, medullary and poorly diff erenƟ ated. Despite the progress in understanding the epidemiology and geneƟ c landscape of thyroid tumors, the diagnosis, prognosis and treatment approach require further improvement. Interleukin-6 (IL-6) is a pro-infl ammatory cytokine with a central role in the regulaƟ on of immune and infl ammatory responses including autoimmune thyroid diseases. Studies have revealed a potenƟ al impact of IL-6 in the development, progression and control of thyroid cancer. The aim of this study was to provide novel aspects for the preoperaƟ ve diff erenƟ al diagnosis and/or prognosis of thyroid cancer. To achieve this, we assessed the circulaƟ ng levels of IL-6 in paƟ ents with benign and malignant thyroid tumors of various histotypes, compared them with healthy volunteers, and correlated the results with clinicopathological parameters. PaƟ ents and Methods: The study included 43 paƟ ents with benign or malignant thyroid tumors, surgically treated at the Center for Endocrine Surgery, Clinical Center of Serbia. IL-6 protein levels were determined in plasma samples by quanƟ taƟ ve ELISA. Parametric and nonparametric staƟ sƟ cal tests were used for data analysis. Results: IL-6 concentraƟ ons in paƟ ents with either TA or carcinoma (PTC, ATC) were signifi cantly higher compared to the healthy volunteers (Mann Whitney test). The highest concentraƟ ons were detected in ATC paƟ ents (Median±SD 15.97±0.71 pg/mL), being signifi cantly higher compared to TA and PTC (2.14±1.34 pg/mL and 1.96±2.12 pg/mL, respecƟ vely). In PTC microcarcinoma, IL-6 was higher compared to controls, but there was no signifi cant diff erence compared to other PTC or TA (Mann Whitney test). The correlaƟ on analysis with clinicopathological parameters in PTC paƟ ents revealed a trend towards the associaƟ on of increased IL-6 plasma levels with the presence of nodal and distant metastases. No other signifi cant associaƟ ons were found. Conclusion: PaƟ ents with thyroid adenoma or carcinoma have increased plasma IL-6 levels that are in proporƟ on with the aggressiveness of the thyroid tumor, suggesƟ ng that IL-6 might be a candidate biomarker for diagnosis and prognosis of thyroid neoplasms.",
publisher = "Belgrade : Serbian Association for Cancer Research",
journal = "Oncology Insights",
title = "Interleukin-6, a potential plasma biomarker for diagnosis and prognosis of thyroid neoplasms",
number = "1",
pages = "83-83",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12634"
}

Todorović, L., Milovanović, J., Mandušić, V., Živaljević, V., Paunović, I.,& Stanojević, B.. (2023). Interleukin-6, a potential plasma biomarker for diagnosis and prognosis of thyroid neoplasms. in Oncology Insights
Belgrade : Serbian Association for Cancer Research.(1), 83-83.
https://hdl.handle.net/21.15107/rcub_vinar_12634

Todorović L, Milovanović J, Mandušić V, Živaljević V, Paunović I, Stanojević B. Interleukin-6, a potential plasma biomarker for diagnosis and prognosis of thyroid neoplasms. in Oncology Insights. 2023;(1):83-83.
https://hdl.handle.net/21.15107/rcub_vinar_12634 .

Todorović, Lidija, Milovanović, Jelena, Mandušić, Vesna, Živaljević, Vladan, Paunović, Ivan, Stanojević, Boban, "Interleukin-6, a potential plasma biomarker for diagnosis and prognosis of thyroid neoplasms" in Oncology Insights, no. 1 (2023):83-83,
https://hdl.handle.net/21.15107/rcub_vinar_12634 .

TY  - JOUR
AU  - Milovanović, Jelena
AU  - Vujasinović, Tijana
AU  - Todorović-Raković, Nataša
AU  - Greenman, John
AU  - Hranisavljević, Jelena
AU  - Radulovic, Marko
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11982
AB  - Background: Vascular endothelial growth factor (VEGF) -A and -C act as multifunctional molecules and growth factors, while VE-cadherin (cadherin 5, CDH5) is the endothelial junction protein. Aim: To assess the relationship between intratumoral VEGF -A, -C and CDH5 levels and clinical outcome, in primary, early-stage, breast cancer patients. Patients and methods: The study included 69 node-negative (N0) breast cancer patients, all of whom had not received any prior hormonal or chemotherapeutic systemic therapy that would affect the course of disease. The median follow-up period was 144 months. Intratumoral mRNA levels of VEGF -A, -C and CDH5 were determined by RT-qPCR. Prognostic performance was evaluated by Cox proportional hazards regression, Kaplan-Meier analysis, as well as by the multivariable approach based on the least absolute shrinkage and selection operator (LASSO) logit regression. Classification of patients into the low and high subgroups was performed using the outcome-oriented cut-off point categorization approach. Results: Of the measured mRNAs, only CDH5 mRNA (t = −2.17; p = 0.04) and VEGF-C mRNA (t = −2.41; p = 0.03) showed significant differences between values in patient subgroups with distant metastasis and those without recurrences, respectively. These t-test results were in agreement with the Cox regression by which CDH5 mRNA reached the most pronounced hazard ratio (HR=2.07; p = 0.05), followed by VEGF-C mRNA (HR=1.59; p = 0.005). HR values above 1.0 indicate that high levels of either CDH5 or VEGF-C mRNAs associated with a higher risk of poor clinical outcome. Distant recurrence incidence was 26% for the CDH5high and 3% for the CDH5low subgroup (Kaplan–Meier analysis). Distant recurrence incidence was 23% for the VEGF-Chigh and 0% for VEGF-Clow subgroup. The independent prognostic value of VEGF-C mRNA was confirmed by LASSO regression. Conclusion: Intratumoral VEGF-A levels did not associate with disease outcome in primary, early-stage, breast cancer patients, whilst raised levels of either CDH5 or VEGF-C prognosticated a high risk of distant metastasis.
T2  - Pathology - Research and Practice
T1  - Vascular endothelial growth factor (VEGF) -A, -C and VE-cadherin as potential biomarkers in early breast cancer patients
VL  - 252
SP  - 154923
DO  - 10.1016/j.prp.2023.154923
ER  - 

@article{
author = "Milovanović, Jelena and Vujasinović, Tijana and Todorović-Raković, Nataša and Greenman, John and Hranisavljević, Jelena and Radulovic, Marko",
year = "2023",
abstract = "Background: Vascular endothelial growth factor (VEGF) -A and -C act as multifunctional molecules and growth factors, while VE-cadherin (cadherin 5, CDH5) is the endothelial junction protein. Aim: To assess the relationship between intratumoral VEGF -A, -C and CDH5 levels and clinical outcome, in primary, early-stage, breast cancer patients. Patients and methods: The study included 69 node-negative (N0) breast cancer patients, all of whom had not received any prior hormonal or chemotherapeutic systemic therapy that would affect the course of disease. The median follow-up period was 144 months. Intratumoral mRNA levels of VEGF -A, -C and CDH5 were determined by RT-qPCR. Prognostic performance was evaluated by Cox proportional hazards regression, Kaplan-Meier analysis, as well as by the multivariable approach based on the least absolute shrinkage and selection operator (LASSO) logit regression. Classification of patients into the low and high subgroups was performed using the outcome-oriented cut-off point categorization approach. Results: Of the measured mRNAs, only CDH5 mRNA (t = −2.17; p = 0.04) and VEGF-C mRNA (t = −2.41; p = 0.03) showed significant differences between values in patient subgroups with distant metastasis and those without recurrences, respectively. These t-test results were in agreement with the Cox regression by which CDH5 mRNA reached the most pronounced hazard ratio (HR=2.07; p = 0.05), followed by VEGF-C mRNA (HR=1.59; p = 0.005). HR values above 1.0 indicate that high levels of either CDH5 or VEGF-C mRNAs associated with a higher risk of poor clinical outcome. Distant recurrence incidence was 26% for the CDH5high and 3% for the CDH5low subgroup (Kaplan–Meier analysis). Distant recurrence incidence was 23% for the VEGF-Chigh and 0% for VEGF-Clow subgroup. The independent prognostic value of VEGF-C mRNA was confirmed by LASSO regression. Conclusion: Intratumoral VEGF-A levels did not associate with disease outcome in primary, early-stage, breast cancer patients, whilst raised levels of either CDH5 or VEGF-C prognosticated a high risk of distant metastasis.",
journal = "Pathology - Research and Practice",
title = "Vascular endothelial growth factor (VEGF) -A, -C and VE-cadherin as potential biomarkers in early breast cancer patients",
volume = "252",
pages = "154923",
doi = "10.1016/j.prp.2023.154923"
}

Milovanović, J., Vujasinović, T., Todorović-Raković, N., Greenman, J., Hranisavljević, J.,& Radulovic, M.. (2023). Vascular endothelial growth factor (VEGF) -A, -C and VE-cadherin as potential biomarkers in early breast cancer patients. in Pathology - Research and Practice, 252, 154923.
https://doi.org/10.1016/j.prp.2023.154923

Milovanović J, Vujasinović T, Todorović-Raković N, Greenman J, Hranisavljević J, Radulovic M. Vascular endothelial growth factor (VEGF) -A, -C and VE-cadherin as potential biomarkers in early breast cancer patients. in Pathology - Research and Practice. 2023;252:154923.
doi:10.1016/j.prp.2023.154923 .

Milovanović, Jelena, Vujasinović, Tijana, Todorović-Raković, Nataša, Greenman, John, Hranisavljević, Jelena, Radulovic, Marko, "Vascular endothelial growth factor (VEGF) -A, -C and VE-cadherin as potential biomarkers in early breast cancer patients" in Pathology - Research and Practice, 252 (2023):154923,
https://doi.org/10.1016/j.prp.2023.154923 . .