TY  - JOUR
AU  - Stanković, Dragana
AU  - Radović, Magdalena
AU  - Stanković, Aljoša
AU  - Mirković, Marija
AU  - Vukadinović, Aleksandar
AU  - Mijović, Milica
AU  - Milanović, Zorana
AU  - Ognjanović, Miloš
AU  - Janković, Drina
AU  - Antić, Bratislav
AU  - Vranješ-Đurić, Sanja
AU  - Savić, Miroslav
AU  - Prijović, Željko
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11387
AB  - As an alternative to classical brachytherapy, intratumoral injection of radionuclide-labeled nanoparticles (nanobrachytherapy, NBT) has been investigated as a superior delivery method over an intravenous route for radionuclide therapy of solid tumors. We created superparamagnetic iron oxide nanoparticles (SPIONs) coated with meso-1,2-dimercaptosuccinic acid (DMSA) and radiolabeled with Lutetium-177 (177Lu), generating 177Lu-DMSA@SPIONs as a potential antitumor agent for nanobrachytherapy. Efficient radiolabeling of DMSA@SPIONS by 177Lu resulted in a stable bond with minimal leakage in vitro. After an intratumoral injection to mouse colorectal CT-26 or breast 4T1 subcutaneous tumors, the nanoparticles remained well localized at the injection site for weeks, with limited leakage. The dose of 3.70 MBq/100 µg/50 µL of 177Lu-DMSA@SPIONs applied intratumorally resulted in a high therapeutic efficacy, without signs of general toxicity. A decreased dose of 1.85 MBq/100 µg/50 µL still retained therapeutic efficacy, while an increased dose of 9.25 MBq/100 µg/50 µL did not significantly benefit the therapy. Histopathology analysis revealed that the 177Lu-DMSA@SPIONs act within a limited range around the injection site, which explains the good therapeutic efficacy achieved by a single administration of a relatively low dose without the need for increased or repeated dosing. Overall, 177Lu-DMSA@SPIONs are safe and potent agents suitable for intra-tumoral administration for localized tumor radionuclide therapy
T2  - Pharmaceutics
T1  - Synthesis, Characterization, and Therapeutic Efficacy of 177Lu-DMSA@SPIONs in Nanobrachytherapy of Solid Tumors
VL  - 15
IS  - 7
SP  - 1943
DO  - 10.3390/pharmaceutics15071943
ER  - 

@article{
author = "Stanković, Dragana and Radović, Magdalena and Stanković, Aljoša and Mirković, Marija and Vukadinović, Aleksandar and Mijović, Milica and Milanović, Zorana and Ognjanović, Miloš and Janković, Drina and Antić, Bratislav and Vranješ-Đurić, Sanja and Savić, Miroslav and Prijović, Željko",
year = "2023",
abstract = "As an alternative to classical brachytherapy, intratumoral injection of radionuclide-labeled nanoparticles (nanobrachytherapy, NBT) has been investigated as a superior delivery method over an intravenous route for radionuclide therapy of solid tumors. We created superparamagnetic iron oxide nanoparticles (SPIONs) coated with meso-1,2-dimercaptosuccinic acid (DMSA) and radiolabeled with Lutetium-177 (177Lu), generating 177Lu-DMSA@SPIONs as a potential antitumor agent for nanobrachytherapy. Efficient radiolabeling of DMSA@SPIONS by 177Lu resulted in a stable bond with minimal leakage in vitro. After an intratumoral injection to mouse colorectal CT-26 or breast 4T1 subcutaneous tumors, the nanoparticles remained well localized at the injection site for weeks, with limited leakage. The dose of 3.70 MBq/100 µg/50 µL of 177Lu-DMSA@SPIONs applied intratumorally resulted in a high therapeutic efficacy, without signs of general toxicity. A decreased dose of 1.85 MBq/100 µg/50 µL still retained therapeutic efficacy, while an increased dose of 9.25 MBq/100 µg/50 µL did not significantly benefit the therapy. Histopathology analysis revealed that the 177Lu-DMSA@SPIONs act within a limited range around the injection site, which explains the good therapeutic efficacy achieved by a single administration of a relatively low dose without the need for increased or repeated dosing. Overall, 177Lu-DMSA@SPIONs are safe and potent agents suitable for intra-tumoral administration for localized tumor radionuclide therapy",
journal = "Pharmaceutics",
title = "Synthesis, Characterization, and Therapeutic Efficacy of 177Lu-DMSA@SPIONs in Nanobrachytherapy of Solid Tumors",
volume = "15",
number = "7",
pages = "1943",
doi = "10.3390/pharmaceutics15071943"
}

Stanković, D., Radović, M., Stanković, A., Mirković, M., Vukadinović, A., Mijović, M., Milanović, Z., Ognjanović, M., Janković, D., Antić, B., Vranješ-Đurić, S., Savić, M.,& Prijović, Ž.. (2023). Synthesis, Characterization, and Therapeutic Efficacy of 177Lu-DMSA@SPIONs in Nanobrachytherapy of Solid Tumors. in Pharmaceutics, 15(7), 1943.
https://doi.org/10.3390/pharmaceutics15071943

Stanković D, Radović M, Stanković A, Mirković M, Vukadinović A, Mijović M, Milanović Z, Ognjanović M, Janković D, Antić B, Vranješ-Đurić S, Savić M, Prijović Ž. Synthesis, Characterization, and Therapeutic Efficacy of 177Lu-DMSA@SPIONs in Nanobrachytherapy of Solid Tumors. in Pharmaceutics. 2023;15(7):1943.
doi:10.3390/pharmaceutics15071943 .

Stanković, Dragana, Radović, Magdalena, Stanković, Aljoša, Mirković, Marija, Vukadinović, Aleksandar, Mijović, Milica, Milanović, Zorana, Ognjanović, Miloš, Janković, Drina, Antić, Bratislav, Vranješ-Đurić, Sanja, Savić, Miroslav, Prijović, Željko, "Synthesis, Characterization, and Therapeutic Efficacy of 177Lu-DMSA@SPIONs in Nanobrachytherapy of Solid Tumors" in Pharmaceutics, 15, no. 7 (2023):1943,
https://doi.org/10.3390/pharmaceutics15071943 . .

TY  - JOUR
AU  - Stanković, Aljoša
AU  - Mihailović, Jasna
AU  - Mirković, Marija D.
AU  - Radović, Magdalena
AU  - Milanović, Zorana
AU  - Ognjanović, Miloš
AU  - Janković, Drina
AU  - Antić, Bratislav
AU  - Mijović, Milica
AU  - Vranješ-Đurić, Sanja
AU  - Prijović, Željko
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9117
AB  - Combined radionuclide therapy with magnetic nanoparticles-mediated hyperthermia has been under research focus as a promising tumor therapy approach. The objective of this study was to investigate the potential of 131I-radiolabeled superparamagnetic iron oxide nanoparticles (SPIONs) prepared as the ~40 nm flower-shaped structures with excellent heating efficiency (specific absorption rate at H0 = 15.9 kA∙m−1 and resonant frequency of 252 kHz was 123.1 W∙g−1) for nano-brachytherapy of tumors. 131I-radiolabeled CC49 antibody attached to SPIONs via reactive groups of 3-aminopropyltriethoxysilane (APTES) provided specificity and long-lasting localized retention after their intratumoral application into LS174T human colon adenocarcinoma xenografts in NOD-SCID mice. The results demonstrate feasibility and effectiveness of magnetic hyperthermia (HT), radionuclide therapy (RT) and their combination (HT + RT) in treating cancer in xenograft models. Combined therapy approach induced a significant (p < 0.01) tumor growth suppression in comparison to untreated groups presented by the tumor volume inhibitory rate (TVIR): 54.38%, 68.77%, 73.00% for HT, RT and HT + RT, respectively in comparison to untreated group and 48.31%, 64,62% and 69,41%, respectively, for the SPIONs-only injected group. Histopathology analysis proved the necrosis and apoptosis in treated tumors without general toxicity. Obtained data support the idea that nano-brachytherapy combined with hyperthermia is a promising approach for effective cancer treatment.
T2  - International Journal of Pharmaceutics
T1  - Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer
VL  - 587
SP  - 119628
DO  - 10.1016/j.ijpharm.2020.119628
ER  - 

@article{
author = "Stanković, Aljoša and Mihailović, Jasna and Mirković, Marija D. and Radović, Magdalena and Milanović, Zorana and Ognjanović, Miloš and Janković, Drina and Antić, Bratislav and Mijović, Milica and Vranješ-Đurić, Sanja and Prijović, Željko",
year = "2020",
abstract = "Combined radionuclide therapy with magnetic nanoparticles-mediated hyperthermia has been under research focus as a promising tumor therapy approach. The objective of this study was to investigate the potential of 131I-radiolabeled superparamagnetic iron oxide nanoparticles (SPIONs) prepared as the ~40 nm flower-shaped structures with excellent heating efficiency (specific absorption rate at H0 = 15.9 kA∙m−1 and resonant frequency of 252 kHz was 123.1 W∙g−1) for nano-brachytherapy of tumors. 131I-radiolabeled CC49 antibody attached to SPIONs via reactive groups of 3-aminopropyltriethoxysilane (APTES) provided specificity and long-lasting localized retention after their intratumoral application into LS174T human colon adenocarcinoma xenografts in NOD-SCID mice. The results demonstrate feasibility and effectiveness of magnetic hyperthermia (HT), radionuclide therapy (RT) and their combination (HT + RT) in treating cancer in xenograft models. Combined therapy approach induced a significant (p < 0.01) tumor growth suppression in comparison to untreated groups presented by the tumor volume inhibitory rate (TVIR): 54.38%, 68.77%, 73.00% for HT, RT and HT + RT, respectively in comparison to untreated group and 48.31%, 64,62% and 69,41%, respectively, for the SPIONs-only injected group. Histopathology analysis proved the necrosis and apoptosis in treated tumors without general toxicity. Obtained data support the idea that nano-brachytherapy combined with hyperthermia is a promising approach for effective cancer treatment.",
journal = "International Journal of Pharmaceutics",
title = "Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer",
volume = "587",
pages = "119628",
doi = "10.1016/j.ijpharm.2020.119628"
}

Stanković, A., Mihailović, J., Mirković, M. D., Radović, M., Milanović, Z., Ognjanović, M., Janković, D., Antić, B., Mijović, M., Vranješ-Đurić, S.,& Prijović, Ž.. (2020). Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer. in International Journal of Pharmaceutics, 587, 119628.
https://doi.org/10.1016/j.ijpharm.2020.119628

Stanković A, Mihailović J, Mirković MD, Radović M, Milanović Z, Ognjanović M, Janković D, Antić B, Mijović M, Vranješ-Đurić S, Prijović Ž. Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer. in International Journal of Pharmaceutics. 2020;587:119628.
doi:10.1016/j.ijpharm.2020.119628 .

Stanković, Aljoša, Mihailović, Jasna, Mirković, Marija D., Radović, Magdalena, Milanović, Zorana, Ognjanović, Miloš, Janković, Drina, Antić, Bratislav, Mijović, Milica, Vranješ-Đurić, Sanja, Prijović, Željko, "Aminosilanized flower-structured superparamagnetic iron oxide nanoparticles coupled to 131I-labeled CC49 antibody for combined radionuclide and hyperthermia therapy of cancer" in International Journal of Pharmaceutics, 587 (2020):119628,
https://doi.org/10.1016/j.ijpharm.2020.119628 . .