Kostić, Milan

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orcid::0000-0003-4680-5835
  • Kostić, Milan (7)
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Author's Bibliography

Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats

Romić, Snježana Đ.; Đorđević, Ana; Tepavčević, Snežana; Ćulafić, Tijana; Stojiljković, Mojca D.; Bursać, Biljana; Stanišić, Jelena; Kostić, Milan; Gligorovska, Ljupka; Korićanac, Goran

(2020)

TY  - JOUR
AU  - Romić, Snježana Đ.
AU  - Đorđević, Ana
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca D.
AU  - Bursać, Biljana
AU  - Stanišić, Jelena
AU  - Kostić, Milan
AU  - Gligorovska, Ljupka
AU  - Korićanac, Goran
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8849
AB  - Both a diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine the effects of the fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether these effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress for 9 weeks. Protein expression and/or phosphorylation of the insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by western blot. GR distribution between cytosolic and nuclear fractions was also analyzed. The fructose-rich diet decreased the level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased the IRS1 content and prevented the fructose diet-induced decrease of the pAkt (Ser473) level. The fructose-rich diet in combination with chronic stress reduced the protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased the protein content of Na+/K+-ATPase α1- and α2-subunits, while after combined treatment the α2 content was at the control level and the α1 content was lower than the control level. The effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights into the effects of chronic stress and a combination of the fructose diet and chronic stress on the studied molecules in the heart.
T2  - Food & Function
T1  - Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats
VL  - 11
IS  - 2
SP  - 1455
EP  - 1466
DO  - 10.1039/C9FO02306B
ER  - 
@article{
author = "Romić, Snježana Đ. and Đorđević, Ana and Tepavčević, Snežana and Ćulafić, Tijana and Stojiljković, Mojca D. and Bursać, Biljana and Stanišić, Jelena and Kostić, Milan and Gligorovska, Ljupka and Korićanac, Goran",
year = "2020",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/8849",
abstract = "Both a diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine the effects of the fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether these effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress for 9 weeks. Protein expression and/or phosphorylation of the insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by western blot. GR distribution between cytosolic and nuclear fractions was also analyzed. The fructose-rich diet decreased the level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased the IRS1 content and prevented the fructose diet-induced decrease of the pAkt (Ser473) level. The fructose-rich diet in combination with chronic stress reduced the protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased the protein content of Na+/K+-ATPase α1- and α2-subunits, while after combined treatment the α2 content was at the control level and the α1 content was lower than the control level. The effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights into the effects of chronic stress and a combination of the fructose diet and chronic stress on the studied molecules in the heart.",
journal = "Food & Function",
title = "Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats",
volume = "11",
number = "2",
pages = "1455-1466",
doi = "10.1039/C9FO02306B"
}
Romić, S. Đ., Đorđević, A., Tepavčević, S., Ćulafić, T., Stojiljković, M. D., Bursać, B., Stanišić, J., Kostić, M., Gligorovska, L.,& Korićanac, G. (2020). Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats.
Food & Function, 11(2), 1455-1466.
https://doi.org/10.1039/C9FO02306B
Romić SĐ, Đorđević A, Tepavčević S, Ćulafić T, Stojiljković MD, Bursać B, Stanišić J, Kostić M, Gligorovska L, Korićanac G. Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats. Food & Function. 2020;11(2):1455-1466
Romić Snježana Đ., Đorđević Ana, Tepavčević Snežana, Ćulafić Tijana, Stojiljković Mojca D., Bursać Biljana, Stanišić Jelena, Kostić Milan, Gligorovska Ljupka, Korićanac Goran, "Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats" Food & Function, 11, no. 2 (2020):1455-1466,
https://doi.org/10.1039/C9FO02306B .
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Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle

Đokić, Vladimir; Janković, Svetlana; Labudović-Borović, Milica; Rakočević, Jelena; Stanišić, Jelena; Rajković, Jovana; Novaković, Radmila; Kostić, Milan; Đurić, Miloš; Gostimirović, Miloš; Gojković-Bukarica, Ljiljana

(2020)

TY  - JOUR
AU  - Đokić, Vladimir
AU  - Janković, Svetlana
AU  - Labudović-Borović, Milica
AU  - Rakočević, Jelena
AU  - Stanišić, Jelena
AU  - Rajković, Jovana
AU  - Novaković, Radmila
AU  - Kostić, Milan
AU  - Đurić, Miloš
AU  - Gostimirović, Miloš
AU  - Gojković-Bukarica, Ljiljana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9048
AB  - Voltage-gated potassium (K ) channels are the largest superfamily of potassium (K) channels. A variety of K channels are expressed in the vascular smooth muscle cells (SMC). Studies have shown that gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) cause various changes in the human umbilical vein (HUV). Recently, we have shown that 4-AP, a nonspecific K 1-4 channel inhibitor, significantly decreases vasorelaxation induced by K channel opener pinacidil in vascular SMCs of the HUVs from normal pregnancies, but not in GDM and PIH. The goal of this study was to provide more detailed insight in the K channel subtypes involved in pinacidil-induced vasodilation of HUVs, as well as to investigate potential alterations of their function and expression during GDM and PIH. Margatoxin, a specific blocker of K 1.2 and K 1.3 channels, significantly antagonized pinacidil-induced vasorelaxation in normal pregnancy, while in HUVs from GDM and PIH that was not the case, indicating damage of K 1.2 and K 1.3 channel function. Immunohistochemistry and Western blot revealed similar expression of K 1.2 channels in all groups. The expression of K 1.3 subunit was significantly decreased in PIH, while it remained unchanged in GDM compared to normal pregnancy. Phrixotoxin, specific blocker of K 4.2 and K 4.3 channels, did not antagonize response to pinacidil in any of the groups. The major novel findings show that margatoxin antagonized pinacidil-induced relaxation in normal pregnancy, but not in GDM and PIH. Decreased expression of K 1.3 channels in HUV during PIH may be important pathophysiological mechanism contributing to an increased risk of adverse pregnancy outcomes.
T2  - European Journal of Pharmacology
T1  - Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle
VL  - 882
SP  - 173281
DO  - 10.1016/j.ejphar.2020.173281
ER  - 
@article{
author = "Đokić, Vladimir and Janković, Svetlana and Labudović-Borović, Milica and Rakočević, Jelena and Stanišić, Jelena and Rajković, Jovana and Novaković, Radmila and Kostić, Milan and Đurić, Miloš and Gostimirović, Miloš and Gojković-Bukarica, Ljiljana",
year = "2020",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/9048",
abstract = "Voltage-gated potassium (K ) channels are the largest superfamily of potassium (K) channels. A variety of K channels are expressed in the vascular smooth muscle cells (SMC). Studies have shown that gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) cause various changes in the human umbilical vein (HUV). Recently, we have shown that 4-AP, a nonspecific K 1-4 channel inhibitor, significantly decreases vasorelaxation induced by K channel opener pinacidil in vascular SMCs of the HUVs from normal pregnancies, but not in GDM and PIH. The goal of this study was to provide more detailed insight in the K channel subtypes involved in pinacidil-induced vasodilation of HUVs, as well as to investigate potential alterations of their function and expression during GDM and PIH. Margatoxin, a specific blocker of K 1.2 and K 1.3 channels, significantly antagonized pinacidil-induced vasorelaxation in normal pregnancy, while in HUVs from GDM and PIH that was not the case, indicating damage of K 1.2 and K 1.3 channel function. Immunohistochemistry and Western blot revealed similar expression of K 1.2 channels in all groups. The expression of K 1.3 subunit was significantly decreased in PIH, while it remained unchanged in GDM compared to normal pregnancy. Phrixotoxin, specific blocker of K 4.2 and K 4.3 channels, did not antagonize response to pinacidil in any of the groups. The major novel findings show that margatoxin antagonized pinacidil-induced relaxation in normal pregnancy, but not in GDM and PIH. Decreased expression of K 1.3 channels in HUV during PIH may be important pathophysiological mechanism contributing to an increased risk of adverse pregnancy outcomes.",
journal = "European Journal of Pharmacology",
title = "Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle",
volume = "882",
pages = "173281",
doi = "10.1016/j.ejphar.2020.173281"
}
Đokić, V., Janković, S., Labudović-Borović, M., Rakočević, J., Stanišić, J., Rajković, J., Novaković, R., Kostić, M., Đurić, M., Gostimirović, M.,& Gojković-Bukarica, L. (2020). Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle.
European Journal of Pharmacology, 882, 173281.
https://doi.org/10.1016/j.ejphar.2020.173281
Đokić V, Janković S, Labudović-Borović M, Rakočević J, Stanišić J, Rajković J, Novaković R, Kostić M, Đurić M, Gostimirović M, Gojković-Bukarica L. Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle. European Journal of Pharmacology. 2020;882:173281
Đokić Vladimir, Janković Svetlana, Labudović-Borović Milica, Rakočević Jelena, Stanišić Jelena, Rajković Jovana, Novaković Radmila, Kostić Milan, Đurić Miloš, Gostimirović Miloš, Gojković-Bukarica Ljiljana, "Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle" European Journal of Pharmacology, 882 (2020):173281,
https://doi.org/10.1016/j.ejphar.2020.173281 .
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Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels

Đokić, Vladimir; Janković-Ražnatović, Svetlana; Novaković, Radmila; Kostić, Milan; Rajković, Jovana; Labudović-Borović, Milica; Rakočević, Jelena T.; Stanišić, Jelena; Đurić, Miloš; Gojković-Bukarica, Ljiljana

(2019)

TY  - JOUR
AU  - Đokić, Vladimir
AU  - Janković-Ražnatović, Svetlana
AU  - Novaković, Radmila
AU  - Kostić, Milan
AU  - Rajković, Jovana
AU  - Labudović-Borović, Milica
AU  - Rakočević, Jelena T.
AU  - Stanišić, Jelena
AU  - Đurić, Miloš
AU  - Gojković-Bukarica, Ljiljana
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8626
AB  - Gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) can jeopardize mother and/or fetus. Vascular ATP-sensitive potassium (KATP) channels most likely participate in the processes of diabetes and hypertension. The aim of this research was to examine whether GDM and PIH cause changes in the expression and function of KATP channels in vascular smooth muscle of human umbilical vein (HUV). Western blot and immunohistochemistry detected significantly decreased expression of Kir6.1 subunit of KATP channels in GDM and PIH, while the expression of SUR2B was unchanged. In GDM, a K+ channel opener, pinacidil caused reduced relaxation of the endothelium-denuded HUVs compared to normal pregnancy. However, its effects in HUVs from PIH subjects were similar to normal pregnancy. In all groups KATP channel blocker glibenclamide antagonized the relaxation of HUV induced by pinacidil without change in the maximal relaxations indicating additional KATP channel-independent mechanisms of pinacidil action. Iberiotoxin, a selective antagonist of large-conductance calcium-activated potassium channels, inhibited the relaxant effect of pinacidil in PIH, but not in normal pregnancy and GDM. Experiments performed in K+-rich solution confirmed the existence of K+-independent effects of pinacidil, which also appear to be impaired in GDM and PIH. Thus, the expression of KATP channels is decreased in GDM and PIH. In GDM, vasorelaxant response of HUV to pinacidil is reduced, while in PIH it remains unchanged. It is very likely that KATP channels modulation and more detailed insight in KATP channel-independent actions of pinacidil may be precious in the therapy of pathological pregnancies. © 2019 Elsevier Inc.
T2  - Experimental and Molecular Pathology
T1  - Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels
VL  - 111
SP  - 104323
DO  - 10.1016/j.yexmp.2019.104323
ER  - 
@article{
author = "Đokić, Vladimir and Janković-Ražnatović, Svetlana and Novaković, Radmila and Kostić, Milan and Rajković, Jovana and Labudović-Borović, Milica and Rakočević, Jelena T. and Stanišić, Jelena and Đurić, Miloš and Gojković-Bukarica, Ljiljana",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8626",
abstract = "Gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) can jeopardize mother and/or fetus. Vascular ATP-sensitive potassium (KATP) channels most likely participate in the processes of diabetes and hypertension. The aim of this research was to examine whether GDM and PIH cause changes in the expression and function of KATP channels in vascular smooth muscle of human umbilical vein (HUV). Western blot and immunohistochemistry detected significantly decreased expression of Kir6.1 subunit of KATP channels in GDM and PIH, while the expression of SUR2B was unchanged. In GDM, a K+ channel opener, pinacidil caused reduced relaxation of the endothelium-denuded HUVs compared to normal pregnancy. However, its effects in HUVs from PIH subjects were similar to normal pregnancy. In all groups KATP channel blocker glibenclamide antagonized the relaxation of HUV induced by pinacidil without change in the maximal relaxations indicating additional KATP channel-independent mechanisms of pinacidil action. Iberiotoxin, a selective antagonist of large-conductance calcium-activated potassium channels, inhibited the relaxant effect of pinacidil in PIH, but not in normal pregnancy and GDM. Experiments performed in K+-rich solution confirmed the existence of K+-independent effects of pinacidil, which also appear to be impaired in GDM and PIH. Thus, the expression of KATP channels is decreased in GDM and PIH. In GDM, vasorelaxant response of HUV to pinacidil is reduced, while in PIH it remains unchanged. It is very likely that KATP channels modulation and more detailed insight in KATP channel-independent actions of pinacidil may be precious in the therapy of pathological pregnancies. © 2019 Elsevier Inc.",
journal = "Experimental and Molecular Pathology",
title = "Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels",
volume = "111",
pages = "104323",
doi = "10.1016/j.yexmp.2019.104323"
}
Đokić, V., Janković-Ražnatović, S., Novaković, R., Kostić, M., Rajković, J., Labudović-Borović, M., Rakočević, J. T., Stanišić, J., Đurić, M.,& Gojković-Bukarica, L. (2019). Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels.
Experimental and Molecular Pathology, 111, 104323.
https://doi.org/10.1016/j.yexmp.2019.104323
Đokić V, Janković-Ražnatović S, Novaković R, Kostić M, Rajković J, Labudović-Borović M, Rakočević JT, Stanišić J, Đurić M, Gojković-Bukarica L. Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels. Experimental and Molecular Pathology. 2019;111:104323
Đokić Vladimir, Janković-Ražnatović Svetlana, Novaković Radmila, Kostić Milan, Rajković Jovana, Labudović-Borović Milica, Rakočević Jelena T., Stanišić Jelena, Đurić Miloš, Gojković-Bukarica Ljiljana, "Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels" Experimental and Molecular Pathology, 111 (2019):104323,
https://doi.org/10.1016/j.yexmp.2019.104323 .
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Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats

Bošković, Maja; Bundalo, Maja M.; Živković, Maja; Stanišić, Jelena; Kostić, Milan; Korićanac, Goran; Stanković, Aleksandra

(2019)

TY  - JOUR
AU  - Bošković, Maja
AU  - Bundalo, Maja M.
AU  - Živković, Maja
AU  - Stanišić, Jelena
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7984
AB  - Harmful effects of fructose-rich diet (FRD) were predominantly observed in males, suggesting protective effects of estrogens. Little is known about AMPK/sirtuin-1 (SIRT1)/forkhead box O3 (FoxO3a)/manganese superoxide dismutase (MnSOD)/catalase signaling in the heart in state of metabolic syndrome and oxidative stress induced by fructose over-consumption. We investigated the effect of 10% FRD on expression of AMPK-SIRT1-FoxO3a-MnSOD/catalase axis in myocardium and potentially beneficial effect of 17β-estradiol replacement. The expression of NADPH oxidase 4 (Nox4) and miRNA-155, unfavorable regulators of this axis, were also investigated. FRD significantly increased AMPK and decreased FoxO3a activity, decreased SIRT1, MnSOD and Nox4 protein expression while E2 reverted these changes, except for Nox4, and increased catalase protein level. E2 diminished Nox4 and MnSOD mRNA level in FRD ovariectomized rats. These results suggest independent response of AMPK and SIRT to FRD treatment. The proposed signaling in the heart should be further investigated in the prooxidative and antioxidative milieu.
T2  - Journal of Functional Foods
T1  - Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats
VL  - 52
SP  - 690
EP  - 698
DO  - 10.1016/j.jff.2018.11.053
ER  - 
@article{
author = "Bošković, Maja and Bundalo, Maja M. and Živković, Maja and Stanišić, Jelena and Kostić, Milan and Korićanac, Goran and Stanković, Aleksandra",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7984",
abstract = "Harmful effects of fructose-rich diet (FRD) were predominantly observed in males, suggesting protective effects of estrogens. Little is known about AMPK/sirtuin-1 (SIRT1)/forkhead box O3 (FoxO3a)/manganese superoxide dismutase (MnSOD)/catalase signaling in the heart in state of metabolic syndrome and oxidative stress induced by fructose over-consumption. We investigated the effect of 10% FRD on expression of AMPK-SIRT1-FoxO3a-MnSOD/catalase axis in myocardium and potentially beneficial effect of 17β-estradiol replacement. The expression of NADPH oxidase 4 (Nox4) and miRNA-155, unfavorable regulators of this axis, were also investigated. FRD significantly increased AMPK and decreased FoxO3a activity, decreased SIRT1, MnSOD and Nox4 protein expression while E2 reverted these changes, except for Nox4, and increased catalase protein level. E2 diminished Nox4 and MnSOD mRNA level in FRD ovariectomized rats. These results suggest independent response of AMPK and SIRT to FRD treatment. The proposed signaling in the heart should be further investigated in the prooxidative and antioxidative milieu.",
journal = "Journal of Functional Foods",
title = "Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats",
volume = "52",
pages = "690-698",
doi = "10.1016/j.jff.2018.11.053"
}
Bošković, M., Bundalo, M. M., Živković, M., Stanišić, J., Kostić, M., Korićanac, G.,& Stanković, A. (2019). Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats.
Journal of Functional Foods, 52, 690-698.
https://doi.org/10.1016/j.jff.2018.11.053
Bošković M, Bundalo MM, Živković M, Stanišić J, Kostić M, Korićanac G, Stanković A. Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats. Journal of Functional Foods. 2019;52:690-698
Bošković Maja, Bundalo Maja M., Živković Maja, Stanišić Jelena, Kostić Milan, Korićanac Goran, Stanković Aleksandra, "Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats" Journal of Functional Foods, 52 (2019):690-698,
https://doi.org/10.1016/j.jff.2018.11.053 .
4
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Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress

Bošković, Maja; Bundalo, Maja M.; Stojiljković, Mojca D.; Kostić, Milan; Živković, Maja; Korićanac, Goran; Stanković, Aleksandra; Životić, Ivan

(2017)

TY  - CONF
AU  - Bošković, Maja
AU  - Bundalo, Maja M.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Živković, Maja
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
AU  - Životić, Ivan
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7179
C3  - Atherosclerosis
T1  - Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress
VL  - 263
SP  - E192
EP  - E192
DO  - 10.1016/j.atherosclerosis.2017.06.616
ER  - 
@conference{
author = "Bošković, Maja and Bundalo, Maja M. and Stojiljković, Mojca D. and Kostić, Milan and Živković, Maja and Korićanac, Goran and Stanković, Aleksandra and Životić, Ivan",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7179",
journal = "Atherosclerosis",
title = "Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress",
volume = "263",
pages = "E192-E192",
doi = "10.1016/j.atherosclerosis.2017.06.616"
}
Bošković, M., Bundalo, M. M., Stojiljković, M. D., Kostić, M., Živković, M., Korićanac, G., Stanković, A.,& Životić, I. (2017). Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress.
Atherosclerosis, 263, E192-E192.
https://doi.org/10.1016/j.atherosclerosis.2017.06.616
Bošković M, Bundalo MM, Stojiljković MD, Kostić M, Živković M, Korićanac G, Stanković A, Životić I. Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress. Atherosclerosis. 2017;263:E192-E192
Bošković Maja, Bundalo Maja M., Stojiljković Mojca D., Kostić Milan, Živković Maja, Korićanac Goran, Stanković Aleksandra, Životić Ivan, "Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress" Atherosclerosis, 263 (2017):E192-E192,
https://doi.org/10.1016/j.atherosclerosis.2017.06.616 .

Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet

Stanišić, Jelena; Korićanac, Goran; Ćulafić, Tijana; Romić, Snježana Đ.; Stojiljković, Mojca D.; Kostić, Milan; Pantelić, Marija; Tepavčević, Snežana

(2016)

TY  - JOUR
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Pantelić, Marija
AU  - Tepavčević, Snežana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/913
AB  - Increase in fructose consumption together with decrease in physical activity contributes to the development of metabolic syndrome and consequently cardiovascular diseases. The current study examined the preventive role of exercise on defects in cardiac insulin signaling and function of endothelial nitric oxide synthase (eNOS) in fructose fed rats. Male Wistar rats were divided into control, sedentary fructose (received 10% fructose for 9 weeks) and exercise fructose (additionally exposed to low intensity exercise) groups. Concentration of triglycerides, glucose, insulin and visceral adipose tissue weight were determined to estimate metabolic syndrome development. Expression and/or phosphorylation of cardiac insulin receptor (IR), insulin receptor substrate 1 (IRS1), tyrosine-specific protein phosphatase 1B (PTP1B), Akt, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and eNOS were evaluated. Fructose overload increased visceral adipose tissue, insulin concentration and homeostasis model assessment index. Exercise managed to decrease visceral adiposity and insulin level and to increase insulin sensitivity. Fructose diet increased level of cardiac PTP1B and pIRS1 (Ser307), while levels of IR and ERK1/2, as well as pIRS1 (Tyr 632), pAkt (Ser473, Thr308) and pERK1/2 were decreased. These disturbances were accompanied by reduced phosphorylation of eNOS at Ser1177. Exercise managed to prevent most of the disturbances in insulin signaling caused by fructose diet (except phosphorylation of IRS1 at Tyr 632 and phosphorylation and protein expression of ERK1/2) and consequently restored function of eNOS. Low intensity exercise could be considered as efficient treatment of cardiac insulin resistance induced by fructose diet. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
T2  - Molecular and Cellular Endocrinology
T1  - Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet
VL  - 420
IS  - C
SP  - 97
EP  - 104
DO  - 10.1016/j.mce.2015.11.032
ER  - 
@article{
author = "Stanišić, Jelena and Korićanac, Goran and Ćulafić, Tijana and Romić, Snježana Đ. and Stojiljković, Mojca D. and Kostić, Milan and Pantelić, Marija and Tepavčević, Snežana",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/913",
abstract = "Increase in fructose consumption together with decrease in physical activity contributes to the development of metabolic syndrome and consequently cardiovascular diseases. The current study examined the preventive role of exercise on defects in cardiac insulin signaling and function of endothelial nitric oxide synthase (eNOS) in fructose fed rats. Male Wistar rats were divided into control, sedentary fructose (received 10% fructose for 9 weeks) and exercise fructose (additionally exposed to low intensity exercise) groups. Concentration of triglycerides, glucose, insulin and visceral adipose tissue weight were determined to estimate metabolic syndrome development. Expression and/or phosphorylation of cardiac insulin receptor (IR), insulin receptor substrate 1 (IRS1), tyrosine-specific protein phosphatase 1B (PTP1B), Akt, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and eNOS were evaluated. Fructose overload increased visceral adipose tissue, insulin concentration and homeostasis model assessment index. Exercise managed to decrease visceral adiposity and insulin level and to increase insulin sensitivity. Fructose diet increased level of cardiac PTP1B and pIRS1 (Ser307), while levels of IR and ERK1/2, as well as pIRS1 (Tyr 632), pAkt (Ser473, Thr308) and pERK1/2 were decreased. These disturbances were accompanied by reduced phosphorylation of eNOS at Ser1177. Exercise managed to prevent most of the disturbances in insulin signaling caused by fructose diet (except phosphorylation of IRS1 at Tyr 632 and phosphorylation and protein expression of ERK1/2) and consequently restored function of eNOS. Low intensity exercise could be considered as efficient treatment of cardiac insulin resistance induced by fructose diet. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
journal = "Molecular and Cellular Endocrinology",
title = "Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet",
volume = "420",
number = "C",
pages = "97-104",
doi = "10.1016/j.mce.2015.11.032"
}
Stanišić, J., Korićanac, G., Ćulafić, T., Romić, S. Đ., Stojiljković, M. D., Kostić, M., Pantelić, M.,& Tepavčević, S. (2016). Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet.
Molecular and Cellular Endocrinology, 420(C), 97-104.
https://doi.org/10.1016/j.mce.2015.11.032
Stanišić J, Korićanac G, Ćulafić T, Romić SĐ, Stojiljković MD, Kostić M, Pantelić M, Tepavčević S. Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet. Molecular and Cellular Endocrinology. 2016;420(C):97-104
Stanišić Jelena, Korićanac Goran, Ćulafić Tijana, Romić Snježana Đ., Stojiljković Mojca D., Kostić Milan, Pantelić Marija, Tepavčević Snežana, "Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet" Molecular and Cellular Endocrinology, 420, no. C (2016):97-104,
https://doi.org/10.1016/j.mce.2015.11.032 .
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Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats

Romić, Snježana Đ.; Tepavčević, Snežana; Žakula, Zorica; Milosavljević, Tijana; Kostić, Milan; Petković, Marijana; Korićanac, Goran

(2014)

TY  - JOUR
AU  - Romić, Snježana Đ.
AU  - Tepavčević, Snežana
AU  - Žakula, Zorica
AU  - Milosavljević, Tijana
AU  - Kostić, Milan
AU  - Petković, Marijana
AU  - Korićanac, Goran
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6053
AB  - To give new insight to alterations of cardiac lipid metabolism accompanied by a fructose-rich diet (FRD), rats of both sexes were exposed to 10 % fructose in drinking water during 9 weeks. The protein level and subcellular localization of the main regulators of cardiac lipid metabolism, such as lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1 alpha), carnitine palmitoyltransferase I (CPTI), and CD36 were studied. Caloric intake in fructose-fed rats (FFR) of both sexes was increased. Circulating triacylglyceroles (TAG) and non-esterified fatty acids were increased in male FFR, while females increased visceral adiposity and blood TAG. Total expression of lipin 1 in cardiac cell lysate and its cytosolic and microsomal level were increased in the hearts of male FFR. PPAR alpha and PGC-1 alpha content were decreased in the nuclear extract. In addition, cardiac deposition of TAG in male FFR was elevated, as well as inhibitory phosphorylation of insulin receptor substrate 1 (IRS-1). In contrast, in female FFR, lipin 1 level was increased in nuclear extract only, while overall CPTI expression and phosphorylation of IRS-1 at serine 307 were decreased. The results of our study suggest that fructose diet causes gender-dependent alterations in cardiac lipid metabolism. Potentially detrimental effects of FRD seem to be limited to male rats. Most of the observed changes might be a consequence of elevated expression and altered localization of lipin 1. Increased inhibitory phosphorylation of IRS-1 is possible link between cardiac lipid metabolism and insulin resistance in FFR.
T2  - Lipids
T1  - Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats
VL  - 49
IS  - 7
SP  - 655
EP  - 663
DO  - 10.1007/s11745-014-3909-4
ER  - 
@article{
author = "Romić, Snježana Đ. and Tepavčević, Snežana and Žakula, Zorica and Milosavljević, Tijana and Kostić, Milan and Petković, Marijana and Korićanac, Goran",
year = "2014",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/6053",
abstract = "To give new insight to alterations of cardiac lipid metabolism accompanied by a fructose-rich diet (FRD), rats of both sexes were exposed to 10 % fructose in drinking water during 9 weeks. The protein level and subcellular localization of the main regulators of cardiac lipid metabolism, such as lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1 alpha), carnitine palmitoyltransferase I (CPTI), and CD36 were studied. Caloric intake in fructose-fed rats (FFR) of both sexes was increased. Circulating triacylglyceroles (TAG) and non-esterified fatty acids were increased in male FFR, while females increased visceral adiposity and blood TAG. Total expression of lipin 1 in cardiac cell lysate and its cytosolic and microsomal level were increased in the hearts of male FFR. PPAR alpha and PGC-1 alpha content were decreased in the nuclear extract. In addition, cardiac deposition of TAG in male FFR was elevated, as well as inhibitory phosphorylation of insulin receptor substrate 1 (IRS-1). In contrast, in female FFR, lipin 1 level was increased in nuclear extract only, while overall CPTI expression and phosphorylation of IRS-1 at serine 307 were decreased. The results of our study suggest that fructose diet causes gender-dependent alterations in cardiac lipid metabolism. Potentially detrimental effects of FRD seem to be limited to male rats. Most of the observed changes might be a consequence of elevated expression and altered localization of lipin 1. Increased inhibitory phosphorylation of IRS-1 is possible link between cardiac lipid metabolism and insulin resistance in FFR.",
journal = "Lipids",
title = "Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats",
volume = "49",
number = "7",
pages = "655-663",
doi = "10.1007/s11745-014-3909-4"
}
Romić, S. Đ., Tepavčević, S., Žakula, Z., Milosavljević, T., Kostić, M., Petković, M.,& Korićanac, G. (2014). Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats.
Lipids, 49(7), 655-663.
https://doi.org/10.1007/s11745-014-3909-4
Romić SĐ, Tepavčević S, Žakula Z, Milosavljević T, Kostić M, Petković M, Korićanac G. Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats. Lipids. 2014;49(7):655-663
Romić Snježana Đ., Tepavčević Snežana, Žakula Zorica, Milosavljević Tijana, Kostić Milan, Petković Marijana, Korićanac Goran, "Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats" Lipids, 49, no. 7 (2014):655-663,
https://doi.org/10.1007/s11745-014-3909-4 .
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